Nabahat BESSADAT, Bruno HAMON, Nelly BATAILLÉ-SIMONEAU, Nisserine HAMINI-KADAR, Mabrouk KIHAL, Philippe SIMONEAU
Symptoms of foliar blight were observed on turfgrass in Oran (Algeria), including yellow chlorotic patches on leaves during the 2020 summer (temperatures between 35 and 40°C). Symptoms extended downward from leaf tips and entire leaves became blighted, leading to irregular discoloured areas that later turned brown. Isolations from infected plants included 214 isolates identified as Curvularia or Bipolaris, based on morphological traits. Other isolates included Fusarium, Myrothecium and Acremonium spp. Three molecular loci, ITS rDNA, gpd and tef1, were amplified and sequenced. Morphological and multi-locus phylogenetic analyses revealed four fungal species viz. B. sorokiniana, C. spicifera, C. verruculosa, C. geniculata, and two additional Curvularia lineages, some of these fungi are reported are first records for Algeria. Koch’s postulates were confirmed by inoculating potted turfgrass with spore suspensions of 16 isolates and re-isolating of the inoculated pathogens from symptomatic tissues. Bipolaris sorokiniana was the most virulent pathogen causing numerous foliar necrotic lesions similar to those observed in the field. Other isolates infected basal leaves only, and caused less severe symptoms. The results show that Curvularia species may be secondary pathogens infecting stressed plants, and that simultaneous occurrence of high temperatures and poor water quality have influenced disease progression. Correct identification of these pathogens is important for applying appropriate and timely disease management.
{"title":"Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in Algeria","authors":"Nabahat BESSADAT, Bruno HAMON, Nelly BATAILLÉ-SIMONEAU, Nisserine HAMINI-KADAR, Mabrouk KIHAL, Philippe SIMONEAU","doi":"10.36253/phyto-14169","DOIUrl":"https://doi.org/10.36253/phyto-14169","url":null,"abstract":"Symptoms of foliar blight were observed on turfgrass in Oran (Algeria), including yellow chlorotic patches on leaves during the 2020 summer (temperatures between 35 and 40°C). Symptoms extended downward from leaf tips and entire leaves became blighted, leading to irregular discoloured areas that later turned brown. Isolations from infected plants included 214 isolates identified as Curvularia or Bipolaris, based on morphological traits. Other isolates included Fusarium, Myrothecium and Acremonium spp. Three molecular loci, ITS rDNA, gpd and tef1, were amplified and sequenced. Morphological and multi-locus phylogenetic analyses revealed four fungal species viz. B. sorokiniana, C. spicifera, C. verruculosa, C. geniculata, and two additional Curvularia lineages, some of these fungi are reported are first records for Algeria. Koch’s postulates were confirmed by inoculating potted turfgrass with spore suspensions of 16 isolates and re-isolating of the inoculated pathogens from symptomatic tissues. Bipolaris sorokiniana was the most virulent pathogen causing numerous foliar necrotic lesions similar to those observed in the field. Other isolates infected basal leaves only, and caused less severe symptoms. The results show that Curvularia species may be secondary pathogens infecting stressed plants, and that simultaneous occurrence of high temperatures and poor water quality have influenced disease progression. Correct identification of these pathogens is important for applying appropriate and timely disease management.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135916962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During 2022, a new disease of bottle gourd, causing leaf mosaic and yellowing symptoms, was observed in a private garden in the Campania region, Southern Italy. Incidence of disease was high (up to 80% of plants with symptoms). Polymerase chain reaction (PCR) with coat protein specific primers to tomato leaf curl New Delhi virus (ToLCNDV) indicated association of a begomovirus with the disease. The sequence comparison and phylogenetic analysis of the complete DNA genome further revealed the virus as within ToLCNDV-ES strain. Nevertheless, phylogenetic relationships showed two distinctive subgroups among ToLCNDV-ES isolates, with subgroup I composed only of ToLCNDV-ES isolates identified in the Campania region, including the isolate found in bottle gourd. The possible evolutionary forces that determined evolution of the two subgroups within the ToLCNDV-ES strain, including the role of the vector and cultural practices, are briefly analyzed and discussed.
{"title":"First report of tomato leaf curl New Delhi virus in Lagenaria siceraria var. longissima in Italy","authors":"Elisa TROIANO, Giuseppe PARRELLA","doi":"10.36253/phyto-14147","DOIUrl":"https://doi.org/10.36253/phyto-14147","url":null,"abstract":"During 2022, a new disease of bottle gourd, causing leaf mosaic and yellowing symptoms, was observed in a private garden in the Campania region, Southern Italy. Incidence of disease was high (up to 80% of plants with symptoms). Polymerase chain reaction (PCR) with coat protein specific primers to tomato leaf curl New Delhi virus (ToLCNDV) indicated association of a begomovirus with the disease. The sequence comparison and phylogenetic analysis of the complete DNA genome further revealed the virus as within ToLCNDV-ES strain. Nevertheless, phylogenetic relationships showed two distinctive subgroups among ToLCNDV-ES isolates, with subgroup I composed only of ToLCNDV-ES isolates identified in the Campania region, including the isolate found in bottle gourd. The possible evolutionary forces that determined evolution of the two subgroups within the ToLCNDV-ES strain, including the role of the vector and cultural practices, are briefly analyzed and discussed.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135905338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fusarium wilt, caused by Fusarium oxysporum f. sp. ciceris (Padwick) Matuo and K. Sato is a major cause for low productivity of chickpea. Presence of multiple pathogenic races makes it difficult for the breeder to screen for Fusarium wilt resistance. Twenty-two chickpea genotypes were grown in Hoagland solution and inoculated with five different F. oxysporum races two isolates of each race), including host and pathogens from the major chickpea growing region of India. The resistant chickpea line “WR 315” showed a “highly resistant” reaction, and the susceptible line “JG 62” showed a “highly susceptible” reaction across all pathogen races and isolates. However, the parent lines “Pusa 372” and “JG 11” showed “susceptible” reactions, while the marker-assisted backcrossing (MABC) lines of “Pusa 372” (IL.11,12,14) and “JG 11” (IL.15,16,17) were superior for assessed characters (lengths of roots and shoots, fresh and dry weights), and were highly resistant to most races. This is the first study to use race specific screening of MABC lines using hydroponic host culture in chickpea.
鹰嘴豆枯萎病(Fusarium oxysporum f. sp. ciceris (Padwick) Matuo and K. Sato)是造成鹰嘴豆产量低下的主要原因。多致病小种的存在使育种者难以筛选枯萎病抗性。在Hoagland溶液中培养了22种基因型的鹰嘴豆，并接种了5个不同的尖孢镰刀菌小种(每个小种2个分离株)，包括来自印度鹰嘴豆主要种植区的宿主和病原体。鹰嘴豆抗性品系“WR 315”和敏感品系“JG 62”在所有致病小种和分离株中均表现为“高度敏感”反应。亲本“Pusa 372”和“JG 11”表现出“敏感”反应，而标记辅助回交(MABC)系“Pusa 372”(IL.11、12、14)和“JG 11”(IL.15、16、17)在评价性状(根、梢长、鲜、干重)方面表现优异，对大多数小种具有较高的抗性。这是首次在鹰嘴豆中使用水培寄主培养技术筛选MABC系的研究。
{"title":"Identification of multi-race Fusarium wilt resistance in chickpea (Cicer arietinum L.) using rapid hydroponic phenotyping","authors":"Jawahar JORBEN, Apoorva RAO, Srinivasa NAGAPPA CHOWLURU, Sakshi TOMAR, Neeraj KUMAR, Chellapilla BHARADWAJ, Basavanagowda SIDDANAGOWDA PATIL, Khela Ram SOREN","doi":"10.36253/phyto-13352","DOIUrl":"https://doi.org/10.36253/phyto-13352","url":null,"abstract":"Fusarium wilt, caused by Fusarium oxysporum f. sp. ciceris (Padwick) Matuo and K. Sato is a major cause for low productivity of chickpea. Presence of multiple pathogenic races makes it difficult for the breeder to screen for Fusarium wilt resistance. Twenty-two chickpea genotypes were grown in Hoagland solution and inoculated with five different F. oxysporum races two isolates of each race), including host and pathogens from the major chickpea growing region of India. The resistant chickpea line “WR 315” showed a “highly resistant” reaction, and the susceptible line “JG 62” showed a “highly susceptible” reaction across all pathogen races and isolates. However, the parent lines “Pusa 372” and “JG 11” showed “susceptible” reactions, while the marker-assisted backcrossing (MABC) lines of “Pusa 372” (IL.11,12,14) and “JG 11” (IL.15,16,17) were superior for assessed characters (lengths of roots and shoots, fresh and dry weights), and were highly resistant to most races. This is the first study to use race specific screening of MABC lines using hydroponic host culture in chickpea.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135836239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Victoria MOREIRA, María Julia CARBONE, Pedro MONDINO, Sandra ALANIZ
Olive anthracnose, caused by Colletotrichum, is an important disease in olive-growing regions, with the most destructive symptoms being fruit rot and blossom blight. Susceptibility of fruit to Colletotrichum increases with maturity, but differences between cultivars and Colletotrichum species have been reported, still information on flower susceptibility during development is scarce. The susceptibility of the olive cultivars Arbequina, Coratina, Frantoio and Picual was evaluated during flower development and fruit maturity to Colletotrichum acutatum s.s., C. nymphaeae, C. fioriniae, C. theobromicola and C. alienum. Susceptibility to anthracnose begins in early stages during flower development and increases during blossoming. Flowers of Arbequina, Coratina and Picual were susceptible, whereas those of Frantoio were moderately susceptible. Green fruit developed less anthracnose than mature fruit. At the green fruit stage, Arbequina and Frantoio were the most susceptible, Coratina was intermediate, and Picual was moderately susceptible, while no differences were found among the cultivars at mature fruit stages. No mayor differences were found among the Colletotrichum species with exception of C. theobromicola, which caused greatest severity at the green fruit stage. Future research should focus on developing anthracnose management strategies to minimize the disease progress from early stages of flower development and fruit ripening, especially in the most susceptible olive cultivars.
{"title":"Colletotrichum infections during flower development and fruit ripening in four olive cultivars","authors":"Victoria MOREIRA, María Julia CARBONE, Pedro MONDINO, Sandra ALANIZ","doi":"10.36253/phyto-14087","DOIUrl":"https://doi.org/10.36253/phyto-14087","url":null,"abstract":"Olive anthracnose, caused by Colletotrichum, is an important disease in olive-growing regions, with the most destructive symptoms being fruit rot and blossom blight. Susceptibility of fruit to Colletotrichum increases with maturity, but differences between cultivars and Colletotrichum species have been reported, still information on flower susceptibility during development is scarce. The susceptibility of the olive cultivars Arbequina, Coratina, Frantoio and Picual was evaluated during flower development and fruit maturity to Colletotrichum acutatum s.s., C. nymphaeae, C. fioriniae, C. theobromicola and C. alienum. Susceptibility to anthracnose begins in early stages during flower development and increases during blossoming. Flowers of Arbequina, Coratina and Picual were susceptible, whereas those of Frantoio were moderately susceptible. Green fruit developed less anthracnose than mature fruit. At the green fruit stage, Arbequina and Frantoio were the most susceptible, Coratina was intermediate, and Picual was moderately susceptible, while no differences were found among the cultivars at mature fruit stages. No mayor differences were found among the Colletotrichum species with exception of C. theobromicola, which caused greatest severity at the green fruit stage. Future research should focus on developing anthracnose management strategies to minimize the disease progress from early stages of flower development and fruit ripening, especially in the most susceptible olive cultivars.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135905337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jaime Nolasco RODRIGUEZ VAZQUEZ, Karim AMMAR, Ignacio SOLIS, Fernando MARTINEZ-MORENO
Leaf rust is a major wheat disease in southern Spain, where durum wheat is an important crop. Until the 2019/2020 season, this disease was effectively managed, as the most widely planted cultivars in southern Spain had effective resistance genes. A problem arose in the spring of 2020, when every farm field and durum wheat trial examined displayed leaf rust symptoms. Leaves had few but large uredinial pustules, different from those of the normal leaf rust caused by Puccinia triticina, and telia developed rapidly after only a few days. The symptoms clearly fitted with P. tritici-duri, another wheat leaf rust species already reported in the western Mediterranean Basin. This species is not new in southern Spain but has never been observed at such high severity on almost every durum wheat cultivar grown in that region. Leaf rust severity was assessed in durum wheat field trials in the 2020, 2021 and 2022 growing seasons in four provinces of southern Spain. During 2020 and 2021, 13 single pustule isolates of leaf rust were also collected from different cultivars of durum wheat. Inoculation of the isolates on a differential host set showed that four different races were present, two being of P. tritici-duri. Only cultivar Calero showed consistent resistance to the races of P. tritici-duri employed in this study.
{"title":"Virulence of Puccinia triticina and Puccinia tritici-duri on durum wheat in southern Spain, from 2020 to 2022","authors":"Jaime Nolasco RODRIGUEZ VAZQUEZ, Karim AMMAR, Ignacio SOLIS, Fernando MARTINEZ-MORENO","doi":"10.36253/phyto-14227","DOIUrl":"https://doi.org/10.36253/phyto-14227","url":null,"abstract":"Leaf rust is a major wheat disease in southern Spain, where durum wheat is an important crop. Until the 2019/2020 season, this disease was effectively managed, as the most widely planted cultivars in southern Spain had effective resistance genes. A problem arose in the spring of 2020, when every farm field and durum wheat trial examined displayed leaf rust symptoms. Leaves had few but large uredinial pustules, different from those of the normal leaf rust caused by Puccinia triticina, and telia developed rapidly after only a few days. The symptoms clearly fitted with P. tritici-duri, another wheat leaf rust species already reported in the western Mediterranean Basin. This species is not new in southern Spain but has never been observed at such high severity on almost every durum wheat cultivar grown in that region. Leaf rust severity was assessed in durum wheat field trials in the 2020, 2021 and 2022 growing seasons in four provinces of southern Spain. During 2020 and 2021, 13 single pustule isolates of leaf rust were also collected from different cultivars of durum wheat. Inoculation of the isolates on a differential host set showed that four different races were present, two being of P. tritici-duri. Only cultivar Calero showed consistent resistance to the races of P. tritici-duri employed in this study.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135905339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Blackleg has been observed in oilseed rape in Tunisia since 2017. Morphological observations, pathogenicity tests, and sequencing of the internal transcribed spacer regions for four fungal isolates from affected plants confirmed the presence of Leptosphaeria maculans and Leptosphaeria biglobosa. These results provide the first record of L. maculans and L. biglobosa as causes of blackleg of oilseed rape in Tunisia.
{"title":"First report of Leptosphaeria maculans and Leptosphaeria biglobosa causing blackleg disease of oilseed rape in Tunisia","authors":"Essia MAGHREBI, Olfa BELDI, Tahani OCHI, Birger KOOPMANN, Hanene CHAABENE, Bochra Amina BAHRI","doi":"10.36253/phyto-13827","DOIUrl":"https://doi.org/10.36253/phyto-13827","url":null,"abstract":"Blackleg has been observed in oilseed rape in Tunisia since 2017. Morphological observations, pathogenicity tests, and sequencing of the internal transcribed spacer regions for four fungal isolates from affected plants confirmed the presence of Leptosphaeria maculans and Leptosphaeria biglobosa. These results provide the first record of L. maculans and L. biglobosa as causes of blackleg of oilseed rape in Tunisia.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135905340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Guarnaccia, C. Kraus, E. Markakis, A. Alves, J. Armengol, A. Eichmeier, S. Compant, D. Gramaje
Production from crops of pome, stone fruit, nut, berry fruit, citrus, grapevine, and olive is increasingly threatened by fungal trunk diseases (FTD). These diseases and the consequent production losses are major problems. Many fungi (including Botryosphaeriaceae, Calosphaeriaceae, Diaporthaceae, Diatrypaceae, Nectriaceae, Phaeomoniellaceae, Pleosporaceae, Togniniaceae, Valsaceae) infect host wood, mainly through wounds and subsequent colonization of woody tissues, causing symptoms such as cankers, gummosis, wood rotting, blight and dieback. Propagative plant material, seedlings and fruit play a significant role in pathogen spread. Several abiotic factors (e.g. shifts in cultural practices and climate change) are involved in the disease development. This paper reviews recent literature on FTD of fruit crops, particularly focusing on the European status of pathogen occurrence. Case studies are described related to diseases of apple, citrus, grapevine, berry, nut and stone fruit, and olive trees. Aspects related to epidemiology and the increase in disease incidence along with the future perspectives on the FTD research are also discussed.
{"title":"Fungal trunk diseases of fruit trees in Europe: pathogens, spread and future directions","authors":"V. Guarnaccia, C. Kraus, E. Markakis, A. Alves, J. Armengol, A. Eichmeier, S. Compant, D. Gramaje","doi":"10.36253/phyto-14167","DOIUrl":"https://doi.org/10.36253/phyto-14167","url":null,"abstract":"Production from crops of pome, stone fruit, nut, berry fruit, citrus, grapevine, and olive is increasingly threatened by fungal trunk diseases (FTD). These diseases and the consequent production losses are major problems. Many fungi (including Botryosphaeriaceae, Calosphaeriaceae, Diaporthaceae, Diatrypaceae, Nectriaceae, Phaeomoniellaceae, Pleosporaceae, Togniniaceae, Valsaceae) infect host wood, mainly through wounds and subsequent colonization of woody tissues, causing symptoms such as cankers, gummosis, wood rotting, blight and dieback. Propagative plant material, seedlings and fruit play a significant role in pathogen spread. Several abiotic factors (e.g. shifts in cultural practices and climate change) are involved in the disease development. This paper reviews recent literature on FTD of fruit crops, particularly focusing on the European status of pathogen occurrence. Case studies are described related to diseases of apple, citrus, grapevine, berry, nut and stone fruit, and olive trees. Aspects related to epidemiology and the increase in disease incidence along with the future perspectives on the FTD research are also discussed.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42427216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The 60th Anniversary of the MPU was celebrated in April 2022, during the 14th MPU Congress in Limassol, Cyprus (AA.VV., 2022). This Special Section of the journal contains four papers from the Congress, including a current topic paper and three reviews. These papers outline modern challenges to plant protection in Mediterranean crops, continuing the long-established traditions of the MPU and Phytopathologia Mediterranea.
{"title":"Preface","authors":"L. Mugnai, R. Falloon","doi":"10.36253/phyto-14192","DOIUrl":"https://doi.org/10.36253/phyto-14192","url":null,"abstract":"The 60th Anniversary of the MPU was celebrated in April 2022, during the 14th MPU Congress in Limassol, Cyprus (AA.VV., 2022). This Special Section of the journal contains four papers from the Congress, including a current topic paper and three reviews. These papers outline modern challenges to plant protection in Mediterranean crops, continuing the long-established traditions of the MPU and Phytopathologia Mediterranea.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42383973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Schianchi, Sandro Flore, Martin Jagunić, S. Serra, V. Prota, D. Vončina
Grapevine virus G (GVG) and grapevine virus H (GVH) (genus Vitivirus) are recently discovered viruses. Analysis of 38 samples from Sardinian grapevine cultivars for the presence of GVG and GVH was carried out using RT-PCR. All samples were also tested for grapevine Pinot gris virus (GPGV) using RT-PCR, and for grapevine leafroll virus -1, -2 and -3, grapevine virus A (GVA) and B (GVB), arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV) and grapevine fleck virus (GFkV) using multiplex RT-PCR. GVG was confirmed in four vines, and GVH was detected in only one sample. In phylogenetic analyses of the coat protein (CP) region, the Sardinian GVG isolates clustered separately from isolates from Croatia and New Zealand. The Sardinian GVH isolate clustered with most sequences from other countries, but with greater affinity to isolates from California (USA) for the CP region, whereas it clustered with isolates from Croatia in the RNA-dependent RNA polymerase (RdRp) region. In addition to GVG and GVH, many samples were coinfected with GVA, viruses from the leafroll complex, and GPGV. This is the first record of GVG and GVH occurring in Italy.
{"title":"Identification of grapevine virus G and grapevine virus H in Sardinia, Italy","authors":"N. Schianchi, Sandro Flore, Martin Jagunić, S. Serra, V. Prota, D. Vončina","doi":"10.36253/phyto-13619","DOIUrl":"https://doi.org/10.36253/phyto-13619","url":null,"abstract":"Grapevine virus G (GVG) and grapevine virus H (GVH) (genus Vitivirus) are recently discovered viruses. Analysis of 38 samples from Sardinian grapevine cultivars for the presence of GVG and GVH was carried out using RT-PCR. All samples were also tested for grapevine Pinot gris virus (GPGV) using RT-PCR, and for grapevine leafroll virus -1, -2 and -3, grapevine virus A (GVA) and B (GVB), arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV) and grapevine fleck virus (GFkV) using multiplex RT-PCR. GVG was confirmed in four vines, and GVH was detected in only one sample. In phylogenetic analyses of the coat protein (CP) region, the Sardinian GVG isolates clustered separately from isolates from Croatia and New Zealand. The Sardinian GVH isolate clustered with most sequences from other countries, but with greater affinity to isolates from California (USA) for the CP region, whereas it clustered with isolates from Croatia in the RNA-dependent RNA polymerase (RdRp) region. In addition to GVG and GVH, many samples were coinfected with GVA, viruses from the leafroll complex, and GPGV. This is the first record of GVG and GVH occurring in Italy.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42715256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Giovani, A. Boutigny, K. Djelouah, A. Fox, A. D’ONGHIA
Strengthening Plant Health research is a major challenge for Mediterranean countries. The diversity and fragmentation of the research landscape in this region have weakened the impacts of national efforts. Mediterranean countries can benefit from coordination of research activities to increase efficiency and impacts. The initiative ‘Plant health research priorities for the Mediterranean region’, led by the International Centre for Advanced Mediterranean Agronomic Studies (CIHEAM Bari)1 and the Euphresco network for phytosanitary research coordination and funding2 can promote convergence of national programmes and optimize the use of the scarce funding available to plant health, thus strengthening international cooperation and increasing the excellence and relevance of research.
{"title":"Plant Health research collaboration in the Mediterranean region: case studies on citrus tristeza virus, tomato brown rugose fruit virus and Xylella fastidiosa","authors":"B. Giovani, A. Boutigny, K. Djelouah, A. Fox, A. D’ONGHIA","doi":"10.36253/phyto-14085","DOIUrl":"https://doi.org/10.36253/phyto-14085","url":null,"abstract":"Strengthening Plant Health research is a major challenge for Mediterranean countries. The diversity and fragmentation of the research landscape in this region have weakened the impacts of national efforts. Mediterranean countries can benefit from coordination of research activities to increase efficiency and impacts. The initiative ‘Plant health research priorities for the Mediterranean region’, led by the International Centre for Advanced Mediterranean Agronomic Studies (CIHEAM Bari)1 and the Euphresco network for phytosanitary research coordination and funding2 can promote convergence of national programmes and optimize the use of the scarce funding available to plant health, thus strengthening international cooperation and increasing the excellence and relevance of research.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41426447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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