Emdadul M. Haque, E. Yamamoto, K. Shirasawa, Hiroaki Tabuchi, U. Yoon, S. Isobe, Masaru Tanaka
: Genetic studies on the purple-fleshed sweetpotato ( Ipomoea batatas L.), which is rich in anthocyanin (AN) in the storage root, were performed by polyploid GWAS based on the allele dosage probability using 59,675 SNPs obtained from 94 F 1 progenies between the cultivars ‘Konaishin’ (which has a high yield but no AN) and ‘Akemurasaki’ (which has a high AN content but low to moderate yield). The distribution of relative AN content was highly biased, with 60% of clones showing a low to undetectable level (A 530 < 0.5). Fifty-nine SNPs from six signals on homologous groups (HGs) 3, 5 (one major and one smaller sig-nal), 7, 13, and 15 were strongly associated with the relative AN content. Twelve SNPs from the major signal and one from the smaller signal of HG 5 were further detected by QTL analysis. In a database search of the AN biosynthesis gene, transcription factors IbMYB1 and IbWD40 and AN structural genes IbF3H and IbDFR were located on HG 5, suggesting that an SNP marker or markers from HG 5 might be tightly linked to candidate gene(s) homologous to one of these transcription factors and AN structural genes as a major factor in determining AN accumulation in the storage roots. These results would enhance our understanding of the underlying genetic basis of AN accumulation in the storage roots of sweetpotatoes, and the SNP markers found here, especially 13 SNPs from HG 5, would be a potential platform for future marker-assisted selection for breeding high-AN sweetpotato varieties.
{"title":"Genetic analyses of anthocyanin content using polyploid GWAS followed by QTL detection in the sweetpotato (Ipomoea batatas L.) storage root","authors":"Emdadul M. Haque, E. Yamamoto, K. Shirasawa, Hiroaki Tabuchi, U. Yoon, S. Isobe, Masaru Tanaka","doi":"10.3117/plantroot.14.11","DOIUrl":"https://doi.org/10.3117/plantroot.14.11","url":null,"abstract":": Genetic studies on the purple-fleshed sweetpotato ( Ipomoea batatas L.), which is rich in anthocyanin (AN) in the storage root, were performed by polyploid GWAS based on the allele dosage probability using 59,675 SNPs obtained from 94 F 1 progenies between the cultivars ‘Konaishin’ (which has a high yield but no AN) and ‘Akemurasaki’ (which has a high AN content but low to moderate yield). The distribution of relative AN content was highly biased, with 60% of clones showing a low to undetectable level (A 530 < 0.5). Fifty-nine SNPs from six signals on homologous groups (HGs) 3, 5 (one major and one smaller sig-nal), 7, 13, and 15 were strongly associated with the relative AN content. Twelve SNPs from the major signal and one from the smaller signal of HG 5 were further detected by QTL analysis. In a database search of the AN biosynthesis gene, transcription factors IbMYB1 and IbWD40 and AN structural genes IbF3H and IbDFR were located on HG 5, suggesting that an SNP marker or markers from HG 5 might be tightly linked to candidate gene(s) homologous to one of these transcription factors and AN structural genes as a major factor in determining AN accumulation in the storage roots. These results would enhance our understanding of the underlying genetic basis of AN accumulation in the storage roots of sweetpotatoes, and the SNP markers found here, especially 13 SNPs from HG 5, would be a potential platform for future marker-assisted selection for breeding high-AN sweetpotato varieties.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69620880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mori, A. Oyanagi, Emdadul M. Haque, K. Kawaguchi, H. Miura, Fumitaka Abe
: The identification of genetic factors affecting deep root systems is needed to improve adaptability to drought stress. In wheat ( Triticum aestivum L.), seminal root angle (SRA), which can be easily measured at the seedling stage, is a useful proxy for understanding the mature root system architecture. In the present study, we attempted to find a genetic variation in the vertical distribution of seminal roots and to determine chromosomal positions involved in a variation of SRA using ditelosomic (Dt) lines and deletion lines of wheat cultivar Chinese Spring (CS). SRA of CS and 31 Dt lines were estimated in a pot experiment using the basket method. When SRA was measured separately for the primary, first pair, and second pair of semi nal roots, it was found that the genetic variation was the largest in the first pair of seminal roots fol lowed by the second pair of seminal roots. In con-trast, there were no genetic differences in the SRA of the primary root between the CS and CS Dt lines. Compared to CS, which had a small SRA, Dt5BL, Dt6AS, Dt6BS, and Dt6DS displayed significantly larger SRA, indicating that the deficient chromosome arms 5BS, 6AL, 6BL, and 6DL appeared to carry quantitative trait loci (QTLs) affect ing SRA. Furthermore, the terminal regions of chromosome arms 6AL and 6BL were found to carry QTLs responsible for a small SRA. Using the QTLs information obtained in this study, novel genes may be isolated from wheat.
{"title":"Terminal regions of chromosome arms 6AL and 6BL carry QTL affecting seminal root angle in wheat (Triticum aestivum L.)","authors":"M. Mori, A. Oyanagi, Emdadul M. Haque, K. Kawaguchi, H. Miura, Fumitaka Abe","doi":"10.3117/plantroot.14.23","DOIUrl":"https://doi.org/10.3117/plantroot.14.23","url":null,"abstract":": The identification of genetic factors affecting deep root systems is needed to improve adaptability to drought stress. In wheat ( Triticum aestivum L.), seminal root angle (SRA), which can be easily measured at the seedling stage, is a useful proxy for understanding the mature root system architecture. In the present study, we attempted to find a genetic variation in the vertical distribution of seminal roots and to determine chromosomal positions involved in a variation of SRA using ditelosomic (Dt) lines and deletion lines of wheat cultivar Chinese Spring (CS). SRA of CS and 31 Dt lines were estimated in a pot experiment using the basket method. When SRA was measured separately for the primary, first pair, and second pair of semi nal roots, it was found that the genetic variation was the largest in the first pair of seminal roots fol lowed by the second pair of seminal roots. In con-trast, there were no genetic differences in the SRA of the primary root between the CS and CS Dt lines. Compared to CS, which had a small SRA, Dt5BL, Dt6AS, Dt6BS, and Dt6DS displayed significantly larger SRA, indicating that the deficient chromosome arms 5BS, 6AL, 6BL, and 6DL appeared to carry quantitative trait loci (QTLs) affect ing SRA. Furthermore, the terminal regions of chromosome arms 6AL and 6BL were found to carry QTLs responsible for a small SRA. Using the QTLs information obtained in this study, novel genes may be isolated from wheat.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69620892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: The mechanical harvesting of processing spinach (spinach used in production of processed food) is potentially becoming a common practice in Japan. Root distribution affects water and nutrient absorption, but how the roots spread in the soil profile during the regrowth period of spinach remains unknown. In this study, we investigate the root systems of processing spinach in topsoil (5 and 15 cm deep) and deeper soil layers (25, 35, 45, and 55 cm deep) cultivated using ratoon harvesting system. The total root length densities (calculated using the average of the densities at 0 and 15 cm horizontal distance from the row for each depth) in the second harvest were more than two-fold greater than those in the first harvest. The root length percentage in the topsoil and root depth indexes indicate that root systems are in shallower soil layers at the second harvest than at first har -vest.
{"title":"Effects of ratoon harvesting on the root systems of processing spinach","authors":"Takayuki Suzuki, Erika Kamada, Takanori Ishii","doi":"10.3117/plantroot.13.23","DOIUrl":"https://doi.org/10.3117/plantroot.13.23","url":null,"abstract":": The mechanical harvesting of processing spinach (spinach used in production of processed food) is potentially becoming a common practice in Japan. Root distribution affects water and nutrient absorption, but how the roots spread in the soil profile during the regrowth period of spinach remains unknown. In this study, we investigate the root systems of processing spinach in topsoil (5 and 15 cm deep) and deeper soil layers (25, 35, 45, and 55 cm deep) cultivated using ratoon harvesting system. The total root length densities (calculated using the average of the densities at 0 and 15 cm horizontal distance from the row for each depth) in the second harvest were more than two-fold greater than those in the first harvest. The root length percentage in the topsoil and root depth indexes indicate that root systems are in shallower soil layers at the second harvest than at first har -vest.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69620754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of strontium on the growth, ion balance, and suberin induction in Solanum lycopersicum","authors":"Takeshi Nagata","doi":"10.3117/plantroot.13.9","DOIUrl":"https://doi.org/10.3117/plantroot.13.9","url":null,"abstract":"","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/plantroot.13.9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69620815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Promotion of adventitious root formation in root-cut mung bean seedlings by a commercial alkaline hydrolysis product of soy oil and its ingredients, unsaturated long-chain fatty acids","authors":"S. Satoh, Taira Okubo, Yoshihiro Nomura","doi":"10.3117/plantroot.13.15","DOIUrl":"https://doi.org/10.3117/plantroot.13.15","url":null,"abstract":"","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69620737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Baba Takashi, Nakaba Satoshi, Noma Satoshi, Funada Ryo, Ban Takuya
: Heterogeneity of individual root traits (heterorhizy) is thought to be one of the most important mechanisms that maintain the functional gradients in fine root systems. It is necessary to know heterorhizy and its relation to the root system architecture in seedlings, because it is the initial form of fine root development. Herein, we investi gated individual root traits and the root system architecture of blueberry seedlings by using the protoxylem grouping. Most individual roots were diarch but some laterals were monarch. Compared to the monarch, the diarch roots had significantly larger diameters and tissues, and over half of them exhibited secondary growth. In other words, frameworks of the blueberry seedlings were diarch, although those of cuttings had been at least triarch as seen in our previous study. The employment of diarch frameworks may optimize investment for root system expansion of blueberry seedlings. Our results imply ontogenetic dynamics of heterorhizy in blueberries.
{"title":"The relationship between individual root anatomy and fine root system development in blueberry seedlings: dominance of diarch roots in initial root systems","authors":"Baba Takashi, Nakaba Satoshi, Noma Satoshi, Funada Ryo, Ban Takuya","doi":"10.3117/PLANTROOT.13.1","DOIUrl":"https://doi.org/10.3117/PLANTROOT.13.1","url":null,"abstract":": Heterogeneity of individual root traits (heterorhizy) is thought to be one of the most important mechanisms that maintain the functional gradients in fine root systems. It is necessary to know heterorhizy and its relation to the root system architecture in seedlings, because it is the initial form of fine root development. Herein, we investi gated individual root traits and the root system architecture of blueberry seedlings by using the protoxylem grouping. Most individual roots were diarch but some laterals were monarch. Compared to the monarch, the diarch roots had significantly larger diameters and tissues, and over half of them exhibited secondary growth. In other words, frameworks of the blueberry seedlings were diarch, although those of cuttings had been at least triarch as seen in our previous study. The employment of diarch frameworks may optimize investment for root system expansion of blueberry seedlings. Our results imply ontogenetic dynamics of heterorhizy in blueberries.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.13.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. F. Ruíz-Herrera, María G. Hernández-Equihua, Daniel Boone-Villa, Gema C. Manuel Jacobo, Asdrúbal Aguilera-Méndez
Biotin is a member of the vitamin Bcomplex family that acts as a cofactor of carboxylases and is essential in the metabolism of all organisms. In addition to its essential metabolic functions, biotin has been suggested to play a critical role in regulating gene expression in plants and animals. However, all studies in plants have been conducted under biotin deficiency. Therefore, we hypothesized that exogenously supplied biotin also exerts non-enzymological functions similar to those reported in animals, regulating root growth and development in Arabidopsis thaliana. To test this hypothesis, we evaluated the effects of the biotin supply on seedlings and analyzed the root architecture. Biotin supplementation inhibited root elongation and promoted lateral root formation in a concentration-dependent manner. Inhibited primary root elongation was correlated with decreased expression of the cell cycle genes CycB1;1:uidA and cell expansion gene pAtExp7::uidA, and depended on the concentration of biotin. Viability tests with AtHisH2B:YFP showed that the meristem was depleted. However, biotin supplementation did not affect the concentration of chlorophyll but had a slight inhibitory effect on foliage growth and increased the production of reactive oxygen species (ROS) at the apex of primary roots. Our study provides the first evidence of functions of biotin supplementation in plants in addition to its catalytic role as an enzyme cofactor, likely advancing our understanding of the biological functions of biotin.
{"title":"Biotin supplementation alters root system architecture and development in Arabidopsis thaliana","authors":"L. F. Ruíz-Herrera, María G. Hernández-Equihua, Daniel Boone-Villa, Gema C. Manuel Jacobo, Asdrúbal Aguilera-Méndez","doi":"10.3117/plantroot.13.29","DOIUrl":"https://doi.org/10.3117/plantroot.13.29","url":null,"abstract":"Biotin is a member of the vitamin Bcomplex family that acts as a cofactor of carboxylases and is essential in the metabolism of all organisms. In addition to its essential metabolic functions, biotin has been suggested to play a critical role in regulating gene expression in plants and animals. However, all studies in plants have been conducted under biotin deficiency. Therefore, we hypothesized that exogenously supplied biotin also exerts non-enzymological functions similar to those reported in animals, regulating root growth and development in Arabidopsis thaliana. To test this hypothesis, we evaluated the effects of the biotin supply on seedlings and analyzed the root architecture. Biotin supplementation inhibited root elongation and promoted lateral root formation in a concentration-dependent manner. Inhibited primary root elongation was correlated with decreased expression of the cell cycle genes CycB1;1:uidA and cell expansion gene pAtExp7::uidA, and depended on the concentration of biotin. Viability tests with AtHisH2B:YFP showed that the meristem was depleted. However, biotin supplementation did not affect the concentration of chlorophyll but had a slight inhibitory effect on foliage growth and increased the production of reactive oxygen species (ROS) at the apex of primary roots. Our study provides the first evidence of functions of biotin supplementation in plants in addition to its catalytic role as an enzyme cofactor, likely advancing our understanding of the biological functions of biotin.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/plantroot.13.29","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69620800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Hop cones are an indispensable raw material used for beer brewing. Since the hop plant is a minor crop, expansion habit of the hop root system has not been well investigated. In this study, we observed and analyzed the root systems of two major hop cultivars in Hokkaido, Little Star and Furano Special, using the wall profile method and root depth index. The results suggest differences in expansion habit of the hop root between the two hop cultivars, which has not been reported previously. The results also indicate that the different responses in cone yield to precipitation between the two hop cultivars may be derived from their differences in root expansion habit. The results of this preliminary study support the view that a better understanding of the hop root system could lead to improved productivity of hops via the development of agronomical techniques or genotypes.
{"title":"Quantitative evaluation of the hop (Humulus lupulus L.) root system based on wall profile method","authors":"K. Koie, Takao Myoda, H. Yoshida, Hirotake Itoh","doi":"10.3117/PLANTROOT.12.11","DOIUrl":"https://doi.org/10.3117/PLANTROOT.12.11","url":null,"abstract":": Hop cones are an indispensable raw material used for beer brewing. Since the hop plant is a minor crop, expansion habit of the hop root system has not been well investigated. In this study, we observed and analyzed the root systems of two major hop cultivars in Hokkaido, Little Star and Furano Special, using the wall profile method and root depth index. The results suggest differences in expansion habit of the hop root between the two hop cultivars, which has not been reported previously. The results also indicate that the different responses in cone yield to precipitation between the two hop cultivars may be derived from their differences in root expansion habit. The results of this preliminary study support the view that a better understanding of the hop root system could lead to improved productivity of hops via the development of agronomical techniques or genotypes.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"12 1","pages":"11-15"},"PeriodicalIF":0.6,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.12.11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Jaloviar, S. Kucbel, J. Vencurik, Mariana Kýpeťová, Zuzana Parobeková, J. Pittner, M. Saniga, Denisa Sedmáková
: In this study we analysed changes in dis tribution and morphological properties of fine roots caused by underplanting of a 110-year-old Norway spruce monoculture by silver fir and common beech. Three different stand structures were investigated: mature established each quantified ic were calculated. Root-to-root interaction of spruce and underplanted species led to differences in verti cal distribution of roots towards the more homoge nous root density in investigated profile with clear shifting of beech and fir roots into deeper soil lay -ers. Cumulative root fractions of fir and beech in up per 20 cm were lower than those of Norway spruce. The share of spruce roots in depth under 20 cm never exceeded 20%. The co-occurrence of beech and spruce in small spots was about twice as fre quent as the co-occurrence of spruce with fir. We found differences in SRL and SSA between broad leaves (beech) and conifers, while the values of beech morphological parameters increased signifi -cantly, when mixed with spruce. We assume that the competitive strategy of beech is based in changing its root morphology, whereas silver fir is inclined to extend its biomass.
{"title":"Underplanted silver fir and common beech cause changes in root stratification and morphology in mature spruce stands","authors":"P. Jaloviar, S. Kucbel, J. Vencurik, Mariana Kýpeťová, Zuzana Parobeková, J. Pittner, M. Saniga, Denisa Sedmáková","doi":"10.3117/PLANTROOT.12.21","DOIUrl":"https://doi.org/10.3117/PLANTROOT.12.21","url":null,"abstract":": In this study we analysed changes in dis tribution and morphological properties of fine roots caused by underplanting of a 110-year-old Norway spruce monoculture by silver fir and common beech. Three different stand structures were investigated: mature established each quantified ic were calculated. Root-to-root interaction of spruce and underplanted species led to differences in verti cal distribution of roots towards the more homoge nous root density in investigated profile with clear shifting of beech and fir roots into deeper soil lay -ers. Cumulative root fractions of fir and beech in up per 20 cm were lower than those of Norway spruce. The share of spruce roots in depth under 20 cm never exceeded 20%. The co-occurrence of beech and spruce in small spots was about twice as fre quent as the co-occurrence of spruce with fir. We found differences in SRL and SSA between broad leaves (beech) and conifers, while the values of beech morphological parameters increased signifi -cantly, when mixed with spruce. We assume that the competitive strategy of beech is based in changing its root morphology, whereas silver fir is inclined to extend its biomass.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"12 1","pages":"21-30"},"PeriodicalIF":0.6,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.12.21","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Legumes are very important plants both ecologically and agriculturally because they are able to interact symbiotically with rhizobia for biological nitrogen fixation and soil fertilization. Medicago truncatula Gaertn. is an important model legume rich in protein. Salinity represents, today, the major cause of land degradation and crop productivity limitation around the world and affects physiology and metabolism in legumes. In this study, we analyzed the physiological and biochemical responses of rootlets in two contrasting ecotypes of Medicago truncatula (Tru 131, tolerant and Jemalong, sensitive) to different level of NaCl, (68, 102 and 137 mM). Results showed that the tolerant ecotype has a lower water potential than Jemalong. Root protein content of Tru 131 was decreased than Jemalong, this can be explained by accumulation of protein oxidation in the sensitive genotype. Moreover, NaCl increased guaiacol peroxidase activity GPX in rootlets of Tru 131, this enzyme has a protective role against the molecules ROS accumulated during oxidative stress. On the other hand, under salt stress the total content of ascorbate (ASC + DHA) and Glutathione (GSH + GSSG) was increased in the tolerant genotype Tru 131 compared to Jemalong. These results show how the tolerant genotype activate the antioxidative defense system at root level against damages caused by oxidative stress under salinity.
{"title":"Physiological and biochemical characterization of rootlets response to salt stress in two Medicago truncatula Gaertn. ecotypes","authors":"A. A. Amouri, E. Gonzalez, Seghir Hadjadj Aoul","doi":"10.3117/PLANTROOT.12.1","DOIUrl":"https://doi.org/10.3117/PLANTROOT.12.1","url":null,"abstract":"Legumes are very important plants both ecologically and agriculturally because they are able to interact symbiotically with rhizobia for biological nitrogen fixation and soil fertilization. Medicago truncatula Gaertn. is an important model legume rich in protein. Salinity represents, today, the major cause of land degradation and crop productivity limitation around the world and affects physiology and metabolism in legumes. In this study, we analyzed the physiological and biochemical responses of rootlets in two contrasting ecotypes of Medicago truncatula (Tru 131, tolerant and Jemalong, sensitive) to different level of NaCl, (68, 102 and 137 mM). Results showed that the tolerant ecotype has a lower water potential than Jemalong. Root protein content of Tru 131 was decreased than Jemalong, this can be explained by accumulation of protein oxidation in the sensitive genotype. Moreover, NaCl increased guaiacol peroxidase activity GPX in rootlets of Tru 131, this enzyme has a protective role against the molecules ROS accumulated during oxidative stress. On the other hand, under salt stress the total content of ascorbate (ASC + DHA) and Glutathione (GSH + GSSG) was increased in the tolerant genotype Tru 131 compared to Jemalong. These results show how the tolerant genotype activate the antioxidative defense system at root level against damages caused by oxidative stress under salinity.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"10 1","pages":"1-10"},"PeriodicalIF":0.6,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.12.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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