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Biological activity of sod - podzolic sandsand soil with meloriative application of hydrogels 水凝胶改良对草化土砂土生物活性的影响
Pub Date : 2021-07-16 DOI: 10.31993/2308-6459-2021-104-2-13961
T. Danilova, Y. Khomyakov, P. Kononchuk
Biological activity of the soil is an indicator characterizing the number of organisms living in the soil, and quantitatively evaluating the results of their vital activity. High biological activity is a factor in increasing soil fertility and its phytosanitary state, achieved by creating optimal living conditions for soil microorganisms: providing nutrients, especially organic matter, moisture, heat and soil aeration. In this paper, we assess the effect of hydrogels of different chemical bases on the microbiological activity of sod-podzolic sandy loam soil. The effect of hydrogels on the microbiological activity of sod-podzolic soil was studied in a microfield experiment in a special installation (“dry place”) under conditions of soil drought and field conditions (Menkovsky branch of the FSBIU API, Gatchinsky district, Leningrad region). The experience was laid with two types of hydrogels (potassium and sodium base) with a single dose (40 g/m2). In the experiment, the indicators of soil biological activity were compared in the following variants: soil - control with the addition of 90 kg ha-1 NPK in the form of an azofoska, hydrogel introduced to a depth of 10–12 cm + N90 P90 K90, hydrogel introduced to a depth of 20–22 cm + N90P90K90. Samples of sod-podzolic sandy loam soil were taken from depths (10–12 cm and 20–22 cm) at the beginning and at the end of the growing season. In the course of the studies, it was found that when the hydrogel is introduced, both in the upper root-inhabited soil layer and in the 20–22 cm layer, favorable conditions are created for the microbiological activity of sod-podzolic sandy loam soil. It has been established that the chemical basis of the hydrogel does not inhibit the activity of the microbial community.
土壤生物活性是表征土壤中生物数量和定量评价其生命活动结果的指标。高生物活性是提高土壤肥力及其植物检疫状态的一个因素,通过为土壤微生物创造最佳生活条件来实现:提供养分,特别是有机物、水分、热量和土壤通气。本文研究了不同化学碱基的水凝胶对灰化沙壤土微生物活性的影响。在土壤干旱和田间条件下(列宁格勒地区Gatchinsky区俄罗斯联邦生物化学工业联合会Menkovsky分会),在特殊装置(“干燥地”)进行了微场试验,研究了水凝胶对灰化土微生物活性的影响。实验采用单剂量(40 g/m2)的两种水凝胶(钾基和钠基)。在试验中,比较了以下几种土壤生物活性指标:土壤控制,以偶氮磷形式添加90 kg hm -1氮磷钾,水凝胶引入深度为10-12 cm + N90P90K90,水凝胶引入深度为20-22 cm + N90P90K90。在生长季开始和结束时分别从10-12 cm和20-22 cm深度采集草粉砂壤土样品。在研究过程中发现,无论在根栖土层上部,还是在20 ~ 22 cm土层,水凝胶的引入都为灰化沙壤土的微生物活性创造了有利条件。已经确定水凝胶的化学基础不会抑制微生物群落的活性。
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引用次数: 0
Sustainability assessment of the resistance of smooth bromegrass varieties to the causative agent of helminthosporiosis on an artificial infectious background 人工感染背景下凤尾草品种对蠕虫孢子病病原菌抗性的可持续性评价
Pub Date : 2021-07-16 DOI: 10.31993/2308-6459-2021-104-2-14934
N. Kostenko
Smooth bromegrass (Bromopsis inermis Holub.) is widespread perennial grass and high quality feed. It is characterized by hight frost resistance. The main direction in breeding work is breeding of varieties with high seed productivity and with increased resistance to diseases. The research was carried out using an artificial infectious background in the immunology laboratory in FWRC FPA (previously, the All-Russian Williams Fodder Research Institute). This research took place in Lobnya, Mytishchi district, Moscow region, in 2018 -2020 years. Long-term phytosanitary monitoring of crops showed that the most common disease of this culture is brown leaf spot (helminthosporium). Seed germination decreases by 21-41% to this patgen. 16 samples of smooth bromegrass was planted in the artificial infectious background to determine their disease resistance in 2018 According to the study carried out, it was found that the disease reaches its peak at the stage of seed ripening. Sample # 2 and sample # 12 on average exceeded the standard variety Fakelny by 12% for resistance to pathogens of helminthosporiosis (among the studied samples, according to three-year data). Sample # 2 and sample # 12 can be recommended for inclusion in the breeding process to create modern intensive type varieties.
凤尾草(broopsis inermis Holub.)是一种分布广泛的多年生牧草和优质饲料。它的特点是高度抗冻性。育种工作的主要方向是选育种子产量高、抗病能力强的品种。该研究在FWRC FPA(以前为全俄威廉姆斯饲料研究所)免疫学实验室的人工感染背景下进行。该研究于2018 -2020年在莫斯科地区梅季什区洛尼亚进行。作物的长期植物检疫监测表明,该栽培最常见的病害是褐叶斑病(helminthosporium)。该病菌使种子发芽率降低21-41%。2018年,在人工感染背景下种植16份凤尾草样品,测定其抗病能力。研究发现,凤尾草病害在种子成熟阶段达到高峰。样本2和样本12对蠕虫孢子病病原体的抗性平均比标准品种Fakelny高出12%(根据三年数据,在所研究样本中)。样品# 2和样品# 12可以推荐纳入育种过程,以创建现代集约型品种。
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引用次数: 1
Assessment of potato varieties preserving at VIR’s collection to (Phytophthora infestans) resistance for late blight in field and laboratory evaluation VIR收集的马铃薯品种对晚疫病抗性的田间评估和实验室评估
Pub Date : 2021-07-16 DOI: 10.31993/2308-6459-2021-104-2-14616
N. Zoteyeva, O. Kosareva
Thirty-eight varieties of potato Solanum tuberosum L. from the N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) collection were studied for leaf and tuber resistance to late blight. Аn aggressive Phytophthora infestans isolate collected from VIR experimental field was used for inoculation at a concentration of 50,000 zoospores/ml. The disease severity was evaluated using a scale from 1 to 9 grades, where grade 9 means resistant. Recent field observations revealed that most of the variety accessions were sensitive to late blight. In the present study, some of the varieties identified as foliar resistant in field evaluation were further tested under artificial inoculation. Resistance of some varieties found in field observations was not confirmed in laboratory assays. The data obtained in tuber inoculation tests did not show a direct correlation between resistance levels in leaves and tubers. Only some varieties possessed both characteristics, while varieties with leaf resistance and tuber sensitivity and with tuber resistance and leaf sensitivity were also identified. To develop potato varieties that combine resistance to late blight with good agronomic characteristics, resistant lines identified in this study can be potentially hybridized with varieties of high consumer value that are susceptible to late blight.
对Vavilov全俄植物遗传资源研究所收集的38个马铃薯品种(Solanum tuberosum L.)的叶片和块茎抗晚疫病性进行了研究。Аn从VIR实验田收集的侵袭性疫霉菌分离株,接种浓度为50,000个游动孢子/ml。疾病严重程度采用1至9级量表进行评估,其中9级表示抗性。最近的田间观察表明,大多数品种对晚疫病敏感。本研究对田间鉴定为叶片抗性的部分品种进行了进一步的人工接种试验。在田间观察中发现的一些品种的抗性未在实验室分析中得到证实。在块茎接种试验中获得的数据没有显示叶片和块茎的抗性水平之间的直接相关。只有部分品种同时具有这两种性状,同时具有叶抗性和块茎敏感性的品种和具有块茎抗性和叶敏感性的品种也有鉴定。为了培育抗晚疫病与优良农艺性状相结合的马铃薯品种,本研究中鉴定的抗性品系可以与易患晚疫病的高消费价值品种进行潜在杂交。
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引用次数: 0
Biodiversity of the Fusarium fungi causing root rot of winter cereals in Belarus 白俄罗斯冬季谷物根腐病镰刀菌的生物多样性
Pub Date : 2021-07-16 DOI: 10.31993/2308-6459-2021-104-2-14631
N. Krupenko, S. Buga, A. G. Zhukovskiy, I. Odintsova, A. A. Zhukovskaya, T. G. Pilat, V. Leshkevich
Fusarium fungi are the main causal agents of root rot of winter cereals in Belarus. As many as 12 different species were identified, with occurrence being dependent on the cereal host species and weather conditions during the growing season. Lack of precipitation from April to July led to decreasing fungal biodiversity. Fusarium pathogen complex on wheat roots was formed by F. culmorum, F. avenaceum, F. equiseti, F. oxysporum, whereas F. equiseti and F. oxysporum prevailed on triticale and rye, and F. solani, F. avenaceum and F. equiseti dominated on barley root systems. The infestation of root with F. oxysporum increased under dry conditions. In contrast, F. culmorum was isolated from root system of wheat and triticale more frequently when rainfall was sufficient. For the first time, F. cerealis and F. tricinctum were isolated from triticale, and F. cerealis from rye only, in the Republic of Belarus.
镰刀菌是白俄罗斯冬季谷物根腐病的主要病原。鉴定出多达12种不同的种类,其发生取决于谷物寄主种类和生长季节的天气条件。4 ~ 7月降水不足导致真菌生物多样性下降。小麦根系镰刀菌复体主要由镰刀菌、镰刀菌、木贼镰刀菌和尖孢镰刀菌组成,黑麦和黑麦上以木贼镰刀菌和尖孢镰刀菌为主,大麦根系上以番茄镰刀菌、木贼镰刀菌和木贼镰刀菌为主。干燥条件下,尖孢镰刀菌对根系的侵染增加。相反,当降雨充足时,小麦和小黑麦的根系分离出镰刀菌的频率更高。在白俄罗斯共和国首次从小黑麦中分离到小麦镰孢菌和tricinctum镰孢菌,并且仅从黑麦中分离到小麦镰孢菌。
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引用次数: 0
Sanitary and epidemiological significance and resistance to insecticides of houseflies Musca domestica (Analytical Literature Review, 2000-2021) 家蝇卫生流行病学意义及对杀虫剂的抗性(文献分析,2000-2021)
Pub Date : 2021-07-16 DOI: 10.31993/2308-6459-2021-104-2-14984
T. A. Davlianidze, O. Eremina
Insect resistance to insecticides has been and remains a serious problem affecting insect control worldwide. The fight against houseflies is an important part of pest control measures due to the ability of insects to carry pathogens of infectious diseases of humans and animals. But many of the chemicals that have been shown to be effective against them cease to work after just a few years of use. The intensive use of insecticides in world practice has led to the development of natural populations of houseflies that are resistant to all groups of insecticides used to control them. This species is one of the ten insect species that have developed resistance to the maximal amount of active substances. The review summarizes and analyzes the data of foreign and Russian authors on the insecticide resistance of houseflies (Musca domestica L.) over the past 20 years. Data on the resistance of house flies to both traditional insecticides and new chemicals are presented. The main mechanisms of insect resistance and the factors of its development are described. Schemes of rotation of insecticides in the controlling winged flies are given. The review analyzed 101 sources from 11 countries of the world.
昆虫对杀虫剂的抗药性一直是并且仍然是影响世界范围内昆虫防治的一个严重问题。家蝇具有携带人畜传染病病原体的能力,是害虫防治的重要组成部分。但许多已被证明对它们有效的化学物质在使用几年后就失效了。在世界实践中,杀虫剂的大量使用导致家蝇自然种群的发展,这些种群对用于控制它们的所有种类的杀虫剂都具有抗药性。本种是对活性物质产生最大抗性的十种昆虫之一。本文对近20年来国内外有关家蝇(Musca domestica L.)杀虫剂抗性的研究资料进行了综述和分析。介绍了家蝇对传统杀虫剂和新型杀虫剂的抗药性。介绍了植物抗虫的主要机理及其产生的因素。提出了防治飞蝇的杀虫剂轮作方案。该报告分析了来自世界11个国家的101个资料来源。
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引用次数: 3
Selection of target genes for pcr diagnostics of Xanthomonas arboricola virulent for cereals and brassicas 谷物和芸苔黄单胞菌毒力pcr诊断靶基因的选择
Pub Date : 2021-07-16 DOI: 10.31993/2308-6459-2021-104-2-14962
E. Kyrova, A. Ignatov
Plant pathogenic xanthomonads virulent to wheat, rye, barley, tomato, sunflower, and brassicas were isolated in Russia in 2001–2008. Physiological tests and multilocus sequence typing analysis confirmed their position within the Xanthomonas arboricola species. The obtained draft genome sequence of representative strain 3004 from barley plants, which is also virulent to sunflower, brassicas, and chestnut, demonstrated an absence of the Type 3 Secretion System T3SS and an evidence for the lateral gene transfer of some other virulence genes from distantly related bacteria. It was concluded that T4SS genes can be used as the target for group-specific PCR analysis of the emerging pathogen. It was proposed to use virD4, virB3, virB4, and virB9 genes to design a detection system. After preliminary experiments with classic PCR for the chosen genes, primers and TaqMan(R) probe were designed to specifically amplify a 121 bp fragment of the VirD4 gene. Amplification products were obtained for all target Xanthomonas arboricola strains and were not detected in other Xanthomonas species, or in other pathogenic or epiphytic bacteria occurring on these host plants. The assay readily detected Xanthomonas arboricola infection in diseased plants and from bacterial colonies isolated on semi-selective media, and was more sensitive and specific than traditional plating methods.
2001-2008年在俄罗斯分离到对小麦、黑麦、大麦、番茄、向日葵和芸苔有毒性的植物致病性黄病菌。生理试验和多位点序列分型分析证实了它们属于树黄单胞菌属。从大麦植物中获得的具有代表性的菌株3004的基因组序列草图,也对向日葵、甘蓝和栗子有毒力,证明了3型分泌系统T3SS的缺失,并证明了一些其他毒力基因从远亲细菌中转移的证据。结果表明,T4SS基因可作为新发病原菌群体特异性PCR分析的靶点。提出利用virD4、virB3、virB4和virB9基因设计检测系统。对所选基因进行经典PCR初步实验后,设计引物和TaqMan(R)探针特异性扩增VirD4基因的121 bp片段。扩增产物在所有靶树黄单胞菌菌株中均检测到,而在其他黄单胞菌种类中或在这些寄主植物上发生的其他致病菌或附生细菌中均未检测到扩增产物。该方法可快速检测病株和半选择性培养基上分离的菌落感染,比传统的电镀方法具有更高的灵敏度和特异性。
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引用次数: 0
The diamondback moth Plutella xylostella: ecological and biological aspects, harmfulness, population control 小菜蛾:生态生物学、危害、种群控制
Pub Date : 2021-04-19 DOI: 10.31993/2308-6459-2021-104-1-14947
Ирина Валерьевна Андреева, Елена Игоревна Шаталова, Алевтина Владимировна Ходакова
Data on prevalence, biological and physiological characteristics of the diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) are presented. Pest damage is described. Reasons causing increase in harmfulness and economic importance of the insect are noted. An increase in the number of generations able to develop during growing season and earlier pest emergence are recorded under conditions of Siberian region. Factors contributing to the insect density dynamics, including plant species and variety, entomophagous arthropods and entomopathogenic microorganisms, have been considered. Range of chemical insecticides recommended for diamondback moth management in Russian Federation has been indicated. An increase in resistance to chemicals and certain entomopathogens in P. xylostella populations in different regions of the world has been recorded. Possibility of pheromone traps exploitation for efficient pest detection and monitoring has been established. Perspectives of novel efficient and safe means of pest density regulation have been defined.
介绍了我国小菜蛾(鳞翅目:小菜蛾科)的流行、生物学和生理特征。描述虫害损害。指出了其危害性和经济重要性增加的原因。在西伯利亚地区的条件下,能够在生长季节发育的代数增加,害虫出现的时间提前。昆虫密度动态的影响因素包括植物种类和品种、食虫节肢动物和昆虫病原微生物。指出了俄罗斯联邦建议用于小菜蛾管理的化学杀虫剂的范围。据记录,世界不同地区小菜蛾种群对化学品和某些昆虫病原体的抗性有所增加。建立了利用信息素诱捕器对害虫进行有效检测和监测的可能性。对新型高效、安全的害虫密度调控方法进行了展望。
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引用次数: 6
Peculiarities of action and retrospective analysis of fungicides efficacy for protection of soft winter wheat against leaf diseases 杀菌剂防治软质冬小麦叶病的作用特点及效果回顾性分析
Pub Date : 2020-12-25 DOI: 10.31993/2308-6459-2020-103-4-13741
N. Krupenko, I. Odintsova
The data on the efficacy of 40 fungicides for protection of soft winter wheat against leaf diseases during 2010–2019 are demonstrated. Biological efficacy is calculated based upon the area under the curve of disease development. Biological and economical efficacy of those compounds has been analyzed depending on the number of components, as well as chemical classes of active agents in those compounds. The highest biological efficacy of one-component fungicides against powdery mildew has been recorded for those containing proquinazid, tebuconazole and metrafenone – 69.9–79.3 %. Among 23 two-component fungicides the higher efficacy (82.1–84.3 %) against powdery mildew has been recorded for the fungicides containing azole combined with morpholine. Biological efficacy against Septoria leaf blotch has varied from 64.7 to 88.0 % depending on fungicide composition. Among three-component fungicides the efficacy against powdery mildew has varied from 59.5 to 82.8 %, and against Septoria leaf blotch it has varied from 59.8 to 89.9 %. As a result of the diseases severity decrease due to the fungicide application the saved yield has reached 9.9 centner of grain per hectare.
研究了2010-2019年40种杀菌剂对软质冬小麦叶片病害的防护效果。生物功效是根据疾病发展曲线下的面积计算的。这些化合物的生物和经济功效已根据成分的数量以及这些化合物中活性物质的化学类别进行了分析。单组分杀菌剂对白粉病的生物防效最高的是含丙喹奈酮、戊康唑和美拉法酮的杀菌剂,为69.9 ~ 79.3%。在23种双组份杀菌剂中,唑啉复合杀菌剂对白粉病的防效最高,达82.1% ~ 84.3%。根据杀菌剂组成的不同,对Septoria叶斑病的生物有效性从64.7%到88.0%不等。三组分杀菌剂对白粉病的防效在59.5% ~ 82.8%之间,对Septoria叶斑病的防效在59.8% ~ 89.9%之间。由于施用杀菌剂降低了病害的严重程度,节约产量达到每公顷9.9粒。
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引用次数: 2
Features of conspecific and heterospecific interactions of greenhouse whitefly Trialeurodes vaporariorum and spider mite Tetranychus urticae on cucumber 温室白蝇与荨麻疹叶螨对黄瓜的同种和异种相互作用特征
Pub Date : 2020-12-25 DOI: 10.31993/2308-6459-2020-103-4-14258
O. Kirillova, V. A. Razdoburdin
Рlant-mediated interactions between greenhouse whitefly Trialeurodes vaporariorum and spider mite Tetranychus urticae have been studied under laboratory experiments on cucumber. The seedlings have been infested using different herbivores densities, when the first true leaf was fully unfolded The response of pests to previously damaged plants has been studied. Whitefly and spider mite plant selection in free-choice bioassay, as well as the larvae number of the whitefly daughter generation and the daily spider mite fecundity have been evaluated in the experiment. We have found, that the antixenotic and antibiotic cucumber properties in regard to the herbivorous arthropods, as a result of herbivore-induced plant defense development, depend on herbivore species and intensity of arthropod damage to plants. Damage to plants by whitefly has affected the behavior and development of a conspecific individuals only. Damage to plants by spider mites has affected both whitefly and spider mite individuals. Analysis of literature on this issue and the results of our research show a specificity of plant responses to arthropod damage, that can be determined by the specific features of herbivorous species and depend on the nature and intensity of the plant damage.
Рlant-mediated通过室内黄瓜试验研究了温室白蝇与荨叶螨的相互作用。当第一片真正的叶子完全展开时,用不同的食草动物密度侵染幼苗,研究了害虫对先前受损植物的反应。在自由选择生物试验中,评价了白蝇和蜘蛛螨的植物选择、白蝇子代幼虫数和蜘蛛螨的日繁殖力。研究发现,黄瓜对食草节肢动物的抗异源性和抗菌性取决于食草动物种类和节肢动物对植物的伤害强度,这是食草动物诱导植物防御发育的结果。粉虱对植物的危害只影响同种个体的行为和发育。蜘蛛螨对植物的危害对粉虱和蜘蛛螨都有影响。对这一问题的文献分析和我们的研究结果表明,植物对节肢动物伤害的反应具有特异性,这可以由草食性物种的具体特征决定,并取决于植物伤害的性质和强度。
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引用次数: 0
Leds and semiochemicals vs. sex pheromones: tests of the european corn borer attractivity in the Krasnodar territory 发光二极管和半化学物质与性信息素:克拉斯诺达尔地区欧洲玉米螟吸引力的测试
Pub Date : 2020-12-25 DOI: 10.31993/2308-6459-2020-103-4-13989
A. Frolov, I. Grushevaya, A. Kononchuk
In two geographical points of the Krasnodar Territory, viz. vil. Botanika, Gulkevichi District (eastern subzone of the Central natural-economic zone of the Krasnodar Territory) and st. Kurchanskaya, Temryuk District (Anapo-Taman natural-economic zone) on industrial plantations of maize, Delta sticky traps supplied with various baits were tested for attractiveness for adults of the European corn borer (ECB), Ostrinia nubilalis Hbn. In contrast to sex pheromones, traps with ‘bisex lure’ (semiochemicals phenylacetaldehyde and 4-methoxy-2-phenethyl alcohol) or LEDs captured a considerable number of females, whose prognostic value was significantly higher than that of males. On maize fields near Botanika traps with ‘bisex lure’ caught moths almost at 20- and traps with LEDs more than 70-fold higher rate as compared to sex pheromones. On maize plantations near Kurchanskaya, where both the saturation of crop rotations with maize fields and the pest population is significantly lower, semiochemicals caught ECB moths at the same rate as sex pheromones, in contrast to LEDs, which attracted insects by an order of magnitude more frequently. The results of the tests indicate that the use of LED traps for monitoring of ECB is promising under the modern economic situation of expanding the territories intended for the production of corn grain.
在克拉斯诺达尔领土的两个地理点,即维尔。在工业玉米种植园的Gulkevichi地区的Botanika (Krasnodar地区中央自然经济区东部分区)和Temryuk地区的st. Kurchanskaya (Anapo-Taman自然经济区),测试了提供各种诱饵的Delta粘性陷阱对欧洲玉米螟(ECB), Ostrinia nubilalis Hbn成虫的吸引力。与性信息素相比,“双性引诱”(半化学物质苯乙醛和4-甲氧基-2-苯乙醇)或led诱捕器捕获了相当数量的雌性,其预测价值明显高于雄性。在Botanika附近的玉米地里,使用“双性诱剂”的诱捕器捕获飞蛾的几率几乎是20%,而使用led诱捕器捕获飞蛾的几率是使用性信息素的70多倍。在库尔尚斯卡亚附近的玉米种植园,玉米田轮作的饱和程度和害虫数量都明显较低,半化学物质捕捉ECB飞蛾的速度与性信息素相同,与led形成鲜明对比,后者吸引昆虫的频率要高一个数量级。试验结果表明,在扩大玉米生产领域的现代经济形势下,使用LED陷阱监测ECB是有希望的。
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引用次数: 3
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