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Estimating canopy chlorophyll in slash pine using multitemporal vegetation indices from uncrewed aerial vehicles (UAVs) 利用无人驾驶飞行器(UAV)的多时植被指数估算斜叶松树冠叶绿素
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-01-08 DOI: 10.1007/s11119-023-10106-9
Qifu Luan, Cong Xu, Xueyu Tao, Lihua Chen, Jingmin Jiang, Yanjie Li

Canopy Chlorophyll Content (CCC) is an important physiological indicator that reflects the growth stage of trees. Accurate estimation of CCC facilitates dynamic monitoring and efficient forest management. In this study, we used high-resolution remote sensing images obtained by uncrewed aerial vehicles (UAVs) equipped with multispectral sensors (red, green, blue, near-infrared, and red-edge) to estimate CCC of lodgepole pine (Pinus elliottii). Our aim was to determine the optimal machine learning model between support vector regression (SVR) and random forest regression (RFR) for predicting CCC and to evaluate the effectiveness of multispectral bands along with 21 vegetation indices (VIs) in the estimation process. Individual tree boundaries were derived from the canopy height model (CHM) based on three-dimensional (3D) point clouds generated using structure from motion. These images, combined with continuous field measurements from January to December, provided comprehensive data for our analysis. The results showed that the SVR method outperformed the RFR method in estimating leaf chlorophyll content (LCC), with fitting R2 values up to 0.692 and RMSE values up to 0.168 mg⋅g−1. Overall, the study highlights the potential of UAV-based remote sensing for multitemporal forest monitoring, offering advances in precision forestry and tree breeding.

树冠叶绿素含量(CCC)是反映树木生长阶段的重要生理指标。准确估算叶绿素含量有助于动态监测和高效森林管理。在这项研究中,我们使用配备多光谱传感器(红、绿、蓝、近红外和红边)的无人驾驶飞行器(UAV)获取的高分辨率遥感图像来估算落羽松(Pinus elliottii)的叶绿素含量。我们的目的是在支持向量回归(SVR)和随机森林回归(RFR)之间确定最佳的机器学习模型来预测 CCC,并评估多光谱波段和 21 种植被指数(VI)在估算过程中的有效性。单棵树的边界是根据利用运动结构生成的三维(3D)点云,从树冠高度模型(CHM)中得出的。这些图像与 1 月至 12 月的连续实地测量相结合,为我们的分析提供了全面的数据。结果表明,在估算叶片叶绿素含量(LCC)方面,SVR 方法优于 RFR 方法,拟合 R2 值高达 0.692,RMSE 值高达 0.168 mg-g-1。总之,该研究强调了基于无人机的遥感技术在多时空森林监测方面的潜力,为精准林业和树木育种提供了进展。
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引用次数: 0
Optimal treatment placement for on-farm experiments: pseudo-Bayesian optimal designs with a linear response plateau model 农场试验的最佳处理位置:采用线性响应高原模型的伪贝叶斯优化设计
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2024-01-07 DOI: 10.1007/s11119-023-10105-w
Davood Poursina, B. Wade Brorsen, Dayton M. Lambert

On-farm experiments are increasingly being used as their costs have decreased with technological advances in collecting, storing, and processing geospatial data. A question that has not been well addressed is what spatial experimental design is best for on-farm experiments when the goal is to estimate a spatially varying coefficients (SVC) model. The focus here is determining the optimal location of treatments to obtain a nearly D-optimal experimental design when estimating a linear plateau model. A pseudo-Bayesian approach is taken here because the field’s site-specific optimal nitrogen value is unknown. Optimal designs are generated, assuming a fixed number of replications for each treatment level. The resulting designs are more efficient than classic Latin square, strip plot, and completely randomized designs. The method consistently produces designs that have 95% efficiency or higher. Random designs had efficiencies varying from 41 to 64% with Latin squares having higher efficiencies and strip plots lower.

随着收集、存储和处理地理空间数据技术的进步,农场试验的成本也在降低,因此农场试验的使用越来越广泛。一个尚未很好解决的问题是,当目标是估算空间变化系数(SVC)模型时,什么样的空间实验设计最适合农场实验。本文的重点是确定处理的最佳位置,以便在估算线性高原模型时获得近似 D 最佳的实验设计。这里采用的是一种伪贝叶斯方法,因为田间特定地点的最佳氮值是未知的。假设每个处理水平都有固定数量的重复,就能生成最优设计。由此产生的设计比传统的拉丁方阵设计、条形小区设计和完全随机设计更有效。该方法产生的设计效率始终保持在 95% 或更高。随机设计的效率从 41% 到 64% 不等,其中拉丁方形设计的效率较高,条形图设计的效率较低。
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引用次数: 0
Potential to reduce the nitrate residue after harvest in maize fields without sacrificing yield through precision nitrogen management 通过精确氮管理减少玉米田收获后硝酸盐残留而不影响产量的潜力
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-30 DOI: 10.1007/s11119-023-10100-1

Abstract

Site-specific nitrogen management has been proposed as a tool to increase crop yield while decreasing nutrient losses to the environment. Many reports can be found on sensing technologies to quantify the variability within a field and the definition of management zones based on the observed variability. However, fewer studies have been dedicated to the selection of the most suitable N fertilizer management scenario: should more or less nutrients be applied in the zones with a lower crop productivity potential? To address this knowledge gap, nine Flemish maize fields were selected as potential candidates for precision fertilization based on the soil maps and historical vegetation index patterns. Within each field, two management zones were identified based on historical vegetation index patterns and electrical conductivity maps, and different fertilization strategies were tested in each zone. The field trial results in terms of yield and soil residual nitrate showed that site-specific N management outperforms the conventional practice only in the fields with temporally stable management zones. In the fields having differences in the physical soil properties (e.g. presence of stones or clay particles), affecting water availability, lower fertilization in zones with a poor soil productivity potential could be recommended. In the fields where the performance of the management zones changes from year to year mainly due to annual variation in precipitation, a risk of incorrect implementation of the precision fertilization concept was identified. Historical NDVI time series serve a good basis to delineate the temporally stable management zones.

摘要 针对具体地点的氮肥管理被认为是提高作物产量同时减少环境养分损失的一种工具。许多报告介绍了用于量化田间变异性的传感技术,以及根据观测到的变异性确定管理区的方法。然而,专门针对如何选择最合适的氮肥管理方案的研究较少:在作物生产潜力较低的区域应该施用更多还是更少的养分?为了填补这一知识空白,我们根据土壤地图和历史植被指数模式,选择了九块佛兰德玉米田作为精准施肥的潜在候选地。在每块田中,根据历史植被指数模式和电导率图确定了两个管理区,并在每个管理区测试了不同的施肥策略。田间试验的产量和土壤残留硝酸盐结果表明,只有在具有时间稳定管理区的田块中,因地制宜的氮肥管理才优于常规做法。在土壤物理特性存在差异(如存在石块或粘粒)、影响水分供应的田块中,建议在土壤生产力潜力较低的区域减少施肥量。在一些田块,管理区的表现每年都会发生变化,主要是由于降水量的年际变化造成的。历史 NDVI 时间序列为划分时间稳定的管理区提供了良好的基础。
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引用次数: 0
Comparing machine learning algorithms for predicting and digitally mapping surface soil available phosphorous: a case study from southwestern Iran 地表土壤可利用磷的预测和数字化绘图的机器学习算法比较:伊朗西南部的一项案例研究
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-27 DOI: 10.1007/s11119-023-10099-5
Saeid Hojati, Asim Biswas, Mojtaba Norouzi Masir

In developing countries like Iran, where information is scarce, understanding the spatial variability of soil available phosphorous (SAP), one of the three major nutrients, is crucial for effective agricultural ecosystem management. This study aimed to predict and digitally map the spatial distribution and related uncertainty of SAP while also assessing the impact of environmental factors on SAP variability in the topsoils. A study area from northern Khuzestan province, Iran was selected as case study area. Three machine learning (ML) models, namely, Random Forest (RF), Artificial Neural Network (ANN), and Support Vector Regression (SVR), were used to develop predictive relationship between surface soil (0–10 cm) SAP content and environmental covariates derived from a digital elevation model and Landsat 8 images. A total of 250 topsoil samples were collected following the conditioned Latin Hypercube Sampling (cLHS) approach and several soil properties were measured in the laboratory. Mean Absolute Error (MAE), Root Mean Square Error (RMSE), and Lin’s Concordance Correlation Coefficient (LCCC) were used to determine the accuracy of models. The findings indicated that the RF algorithm demonstrated the most favorable performance, with a mean absolute error (MAE) of 0.85 mg SAP kg−1, the lowest root mean square error (RMSE) of 0.99 mg SAP kg−1, and the highest linear correlation coefficient (LCCC) values of 0.96. This suggests that the RF algorithm had the least tendency to overestimate or underestimate SAP contents compared to other methods. Consequently, the RF algorithm was selected as the optimal choice. Predictive ML models were employed to digitally map SAP contents within the region. Spatial patterns of SAP contents showed an increasing gradient from west to east. The spatial variability information provides a basis for developing sustainable production system in the area.

在伊朗等信息匮乏的发展中国家,了解三大营养元素之一的土壤可利用磷(SAP)的空间变化对于有效的农业生态系统管理至关重要。本研究旨在预测和数字化绘制 SAP 的空间分布和相关不确定性,同时评估环境因素对表层土壤中 SAP 变化的影响。研究选取了伊朗胡齐斯坦省北部的一个研究区域作为案例研究区。研究人员使用了三种机器学习(ML)模型,即随机森林(RF)、人工神经网络(ANN)和支持向量回归(SVR),来建立表层土壤(0-10 厘米)SAP 含量与数字高程模型和 Landsat 8 图像中的环境协变量之间的预测关系。采用条件拉丁超立方取样法(cLHS)共采集了 250 个表层土壤样本,并在实验室测量了多个土壤特性。采用平均绝对误差 (MAE)、均方根误差 (RMSE) 和林氏协和相关系数 (LCCC) 来确定模型的准确性。研究结果表明,RF 算法表现最出色,其平均绝对误差(MAE)为 0.85 mg SAP kg-1,均方根误差(RMSE)最低,为 0.99 mg SAP kg-1,线性相关系数(LCCC)最高,为 0.96。这表明,与其他方法相比,射频算法高估或低估 SAP 含量的倾向最小。因此,射频算法被选为最佳选择。采用预测性 ML 模型对区域内的 SAP 含量进行了数字化测绘。SAP 含量的空间模式呈现出由西向东递增的梯度。空间变化信息为该地区发展可持续生产系统提供了依据。
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引用次数: 0
Enhancing direct-seeded rice yield prediction using UAV-derived features acquired during the reproductive phase 利用无人机在生育期获取的特征加强直播水稻产量预测
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-21 DOI: 10.1007/s11119-023-10103-y
Guodong Yang, Yaxing Li, Shen Yuan, Changzai Zhou, Hongshun Xiang, Zhenqing Zhao, Qiaorong Wei, Qingshan Chen, Shaobing Peng, Le Xu

Pre-harvest yield prediction of direct-seeded rice is critical for guiding crop interventions and food security assessment in precision agriculture. Technology advances in unmanned aerial vehicle (UAV)-based remote sensing has provided an unprecedented opportunity to efficiently retrieve crop growth parameters instead of labor-intensive ground measurements. This study is aiming to evaluate the feasibility of fusing multi-temporal UAV-derived features collected at critical phenological stages in forecasting direct-seeded rice yield across different cultivars and nitrogen (N) management. The results showed that RGB sensor-derived canopy volume, canopy coverage, and spectral features including RBRI, WI etc., were identified to be most sensitive to the differences in aboveground biomass and grain yield. Heading stage was the suitable time for estimating yield performance (R2 = 0.75) for mono-temporal UAV observation. By contrast, multi-temporal features fusion could remarkably enhance the yield prediction accuracy. Moreover, the yield prediction accuracy can be further improved by integrating UAV features collected at panicle initiation and heading stages (i.e., rice reproductive phase) compared to multi-temporal features fusion (R2 increased from 0.82 to 0.85 and RMSE decreased from 35.1 to 31.5 g m−2). This can be attributed to the fact that the biomass accumulation during the reproductive phase was closely associated to the total spikelets and final yield. By using this proposed approach, the predicted yield showed a good spatial consistency with the measured yield across different cultivars and N management, and yield prediction error in the most of the plots (114 of 128 plots) was less than 45 g m−2. In summary, this study highlights that the reproductive phase is the optimal time window for UAV observing, which provides an effective method for accurate pre-harvest yield prediction of direct-seeded rice in precision agriculture.

直播水稻收获前的产量预测对于指导作物干预和精准农业中的粮食安全评估至关重要。基于无人飞行器(UAV)的遥感技术的进步提供了一个前所未有的机会,可以有效地检索作物生长参数,而不是进行劳动密集型的地面测量。本研究旨在评估融合在关键物候期收集的多时空无人机衍生特征预测不同栽培品种直播水稻产量和氮素(N)管理的可行性。结果表明,从 RGB 传感器获得的冠层体积、冠层覆盖率和光谱特征(包括 RBRI、WI 等)对地上生物量和谷物产量的差异最为敏感。在单时相无人机观测中,穗期是估算产量表现的合适时间(R2 = 0.75)。相比之下,多时相特征融合可显著提高产量预测精度。此外,与多时空特征融合相比,整合在圆锥花序始穗期和抽穗期(即水稻生育期)采集的无人机特征可进一步提高产量预测精度(R2 从 0.82 提高到 0.85,RMSE 从 35.1 g m-2 降低到 31.5 g m-2)。这可能是因为生殖期的生物量积累与总穗数和最终产量密切相关。使用这种方法,在不同栽培品种和氮管理条件下,预测产量与实测产量在空间上表现出良好的一致性,大多数地块(128 块地中的 114 块)的产量预测误差小于 45 g m-2。总之,本研究强调了生育期是无人机观测的最佳时间窗口,为精准农业中直播水稻收获前的精确产量预测提供了有效方法。
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引用次数: 0
End-to-end 3D CNN for plot-scale soybean yield prediction using multitemporal UAV-based RGB images 利用基于无人机的多时态 RGB 图像进行小区尺度大豆产量预测的端到端 3D CNN
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-21 DOI: 10.1007/s11119-023-10096-8
Sourav Bhadra, Vasit Sagan, Juan Skobalski, Fernando Grignola, Supria Sarkar, Justin Vilbig

Crop yield prediction from UAV images has significant potential in accelerating and revolutionizing crop breeding pipelines. Although convolutional neural networks (CNN) provide easy, accurate and efficient solutions over traditional machine learning models in computer vision applications, a CNN training requires large number of ground truth data, which is often difficult to collect in the agricultural context. The major objective of this study was to develope an end-to-end 3D CNN model for plot-scale soybean yield prediction using multitemporal UAV-based RGB images with approximately 30,000 sample plots. A low-cost UAV-RGB system was utilized and multitemporal images from 13 different experimental fields were collected at Argentina in 2021. Three commonly used 2D CNN architectures (i.e., VGG, ResNet and DenseNet) were transformed into 3D variants to incorporate the temporal data as the third dimension. Additionally, multiple spatiotemporal resolutions were considered as data input and the CNN architectures were trained with different combinations of input shapes. The results reveal that: (a) DenseNet provided the most efficient result (R2 0.69) in terms of accuracy and model complexity, followed by VGG (R2 0.70) and ResNet (R2 0.65); (b) Finer spatiotemporal resolution did not necessarily improve the model performance but increased the model complexity, while the coarser resolution achieved comparable results; and (c) DenseNet showed lower clustering patterns in its prediction maps compared to the other models. This study clearly identifies that multitemporal observation with UAV-based RGB images provides enough information for the 3D CNN architectures to accurately estimate soybean yield non-destructively and efficiently.

利用无人机图像预测作物产量在加速和革新作物育种流程方面具有巨大潜力。虽然在计算机视觉应用中,卷积神经网络(CNN)比传统的机器学习模型提供了简单、准确和高效的解决方案,但 CNN 的训练需要大量地面实况数据,而这些数据在农业环境中往往难以收集。本研究的主要目的是开发一个端到端的三维 CNN 模型,利用基于无人机的多时态 RGB 图像(约有 30,000 个样本地块)进行地块尺度的大豆产量预测。该研究使用了低成本的 UAV-RGB 系统,并于 2021 年在阿根廷收集了 13 块不同试验田的多时相图像。将三种常用的二维 CNN 架构(即 VGG、ResNet 和 DenseNet)转换为三维变体,将时间数据作为第三维。此外,还将多种时空分辨率作为数据输入,并使用不同的输入形状组合训练 CNN 架构。结果显示(a) 就准确度和模型复杂度而言,DenseNet 提供了最有效的结果(R2 0.69),其次是 VGG(R2 0.70)和 ResNet(R2 0.65);(b) 更精细的时空分辨率并不一定能提高模型性能,但会增加模型复杂度,而更粗糙的分辨率则能达到相当的结果;以及 (c) 与其他模型相比,DenseNet 在其预测图中显示了较低的聚类模式。这项研究清楚地表明,利用基于无人机的 RGB 图像进行多时观测可为三维 CNN 架构提供足够的信息,从而非破坏性地、高效地准确估算大豆产量。
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引用次数: 0
Retinanet_G2S: a multi-scale feature fusion-based network for fruit detection of punna navel oranges in complex field environments Retinanet_G2S:基于多尺度特征融合的网络,用于在复杂的田间环境中检测番泻叶脐橙果实
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-19 DOI: 10.1007/s11119-023-10098-6
Hongxing Peng, Hu Chen, Xin Zhang, Huanai Liu, Keyin Chen, Juntao Xiong

In the natural environment, the detection and recognition process of Punna navel orange fruit using machine vision systems is affected by many factors, such as complex background, uneven light illumination, occlusions of branches and leaves and large variations in fruit size. To solve these problems of low accuracy in fruit detection and poor robustness of the detection algorithm in the field conditions, a new object detection algorithm, named Retinanet_G2S, was proposed in this paper based on the modified Retinanet network. The images of Punna navel orange were collected with Microsoft Kinect V2 in the uncontrolled environment. Firstly, a new Res2Net-GF network was designed to replace the section of feature extraction in the original Retinanet, which can potentially improve the learning ability of target features of the trunk network. Secondly, a multi-scale cross-regional feature fusion grids network was designed to replace the feature pyramid network module in the original Retinanet, which could enhance the ability of feature information fusion among different scales of the feature pyramid. Finally, the original border regression localization method in Retinanet network was optimized based on the accurate boundary box regression algorithm. The study results showed that, compared with the original Retinanet network, Retinanet_G2S improved mAP, mAP50, mAP75, mAPS, mAPM and mAPL by 3.8%, 1.7%, 5.8%, 2.4%, 2.1% and 5.5%, respectively. Moreover, compared with 7 types of classic object detection models, including SSD, YOLOv3, CenterNet, CornerNet, FCOS, Faster-RCNN and Retinanet, the average increase in mAP of Retinanet_G2S was 9.11%. Overall, Retinanet_G2S showed a promising optimization effect, particularly for the detection of small targets and overlapping fruits.

在自然环境中,使用机器视觉系统检测和识别蓬莱脐橙果实的过程会受到很多因素的影响,如复杂的背景、不均匀的光照、枝叶的遮挡以及果实大小的巨大变化等。为了解决果实检测精度低、检测算法在现场条件下鲁棒性差等问题,本文基于改进的 Retinanet 网络提出了一种新的物体检测算法,命名为 Retinanet_G2S。本文使用 Microsoft Kinect V2 在非受控环境下采集蓬莱脐橙的图像。首先,设计了一个新的 Res2Net-GF 网络来替代原有 Retinanet 网络中的特征提取部分,从而有可能提高主干网络对目标特征的学习能力。其次,设计了一个多尺度跨区域特征融合网格网络,以替代原有 Retinanet 中的特征金字塔网络模块,从而提高不同尺度特征金字塔之间的特征信息融合能力。最后,基于精确的边界盒回归算法,优化了 Retinanet 网络中原有的边界回归定位方法。研究结果表明,与原始 Retinanet 网络相比,Retinanet_G2S 的 mAP、mAP50、mAP75、mAPS、mAPM 和 mAPL 分别提高了 3.8%、1.7%、5.8%、2.4%、2.1% 和 5.5%。此外,与 SSD、YOLOv3、CenterNet、CornerNet、FCOS、Faster-RCNN 和 Retinanet 等 7 种经典物体检测模型相比,Retinanet_G2S 的 mAP 平均提高了 9.11%。总体而言,Retinanet_G2S 显示出了良好的优化效果,尤其是在检测小目标和重叠果实方面。
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引用次数: 0
Design, implementation and validation of a sensor-based precise airblast sprayer to improve pesticide applications in orchards 设计、实施和验证基于传感器的精确喷气式喷雾器,以改进果园中的农药施用
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-18 DOI: 10.1007/s11119-023-10097-7
Bernat Salas, Ramón Salcedo, Francisco Garcia-Ruiz, Emilio Gil

An orchard sprayer prototype running a variable-rate algorithm to adapt the spray volume to the canopy characteristics (dimensions, shape and leaf density) in real-time was designed and implemented. The developed machine was able to modify the application rate by using an algorithm based on the tree row volume, in combination with a newly coefficient defined as Density Factor (Df). Variations in the canopy characteristics along the row crop were electronically measured using six ultrasonic sensors (three per sprayer side). These differences in foliage structure were used to adjust the flow rate of the nozzles by merging the ultrasonic sensors data and the forward speed information received from the on-board GNSS. A set of motor-valves was used to regulate the final amount of sprayed liquid. Laboratory and field tests using artificial canopy were arranged to calibrate and select the optimal ultrasonic sensor configuration (width beam and signal pre-processing method) that best described the physical canopy properties. Results indicated that the sensor setup with a medium beam width offered the most appropriate characterization of trees in terms of width and Df. The experimental sprayer was also able to calculate the application rate automatically depending on changes on target trees. In general, the motor valves demonstrated adequate capability to supply and control the required liquid pressure at all times, mainly when spraying in a range between 4.0 and 14.0 MPa. Further work is required on the equipment, such as designing field efficiency tests for the sprayer or refining the accuracy of Df.

设计并实施了一种果园喷雾器原型,它采用变速算法,可根据树冠特征(尺寸、形状和叶片密度)实时调整喷洒量。所开发的机器能够通过使用基于树行体积的算法,结合新定义的密度系数(Df)来修改喷洒量。使用六个超声波传感器(喷雾器每侧三个)对行间作物树冠特征的变化进行电子测量。通过合并超声波传感器数据和机载全球导航卫星系统接收到的前进速度信息,利用叶面结构的这些差异来调整喷嘴的流量。一套电动阀用于调节最终喷洒的液体量。利用人工树冠进行了实验室和实地测试,以校准和选择最能描述树冠物理特性的最佳超声波传感器配置(波束宽度和信号预处理方法)。结果表明,中等波束宽度的传感器设置能最恰当地描述树木的宽度和 Df。实验喷雾器还能根据目标树木的变化自动计算施药量。总的来说,电动阀在任何时候都有足够的能力提供和控制所需的液体压力,主要是在 4.0 至 14.0 兆帕的范围内进行喷洒时。还需要对设备做进一步的改进,如设计喷雾器的现场效率测试或改进 Df 的精度。
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引用次数: 0
High-throughput phenotyping of individual plant height in an oilseed rape population based on Mask-RCNN and UAV images 基于 Mask-RCNN 和无人机图像的油菜群体单株高度高通量表型分析
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.1007/s11119-023-10095-9
Yutao Shen, Xuqi Lu, Mengqi Lyu, Hongyu Zhou, Wenxuan Guan, Lixi Jiang, Yuhong He, Haiyan Cen

Plant height, a key agronomic trait, affects crop structure, photosynthesis, and thus the final yield and seed quality. The combination of digital cameras on unmanned aerial vehicles (UAVs) and use of structure from motion have enabled high-throughput crop canopy height estimation. However, the focus of prior research has mainly been on plot-level height prediction, neglecting precise estimations for individual plants. This study aims to explore the potential of UAV RGB images with mask region-based convolutional neural network (Mask-RCNN) for high-throughput phenotyping of individual-level height (IH) in oilseed rape at different growth stages. Field-measured height (FH) of nine sampling plants in each subplot of the 150 subplots was obtained by manual measurement after the UAV flight. An instance segmentation model for oilseed rape with data augmentation based on the Mask-RCNN model was developed. The IHs were then used to obtain plot-level height based on individual-level height (PHIH). The results show that Mask-RCNN performed better than the conventional Otsu method with the F1 score increased by 60.8% and 26.6% under high and low weed pressure, respectively. The trained model with data augmentation achieved accurate crop height estimation based on overexposed and underexposed UAV images, indicating the model’s applicability in practical scenarios. The PHIH can be predicted with the determination coefficient (r2) of 0.992, root mean square error (RMSE) of 4.03 cm, relative root mean square error (rRMSE) of 7.68%, which outperformed the results in the reported studies, especially in the late bolting stage. The IHs of the whole growth stages of oilseed can be predicted by this method with an r2 of 0.983, RMSE of 2.60 cm, and rRMSE of 7.14%. Furthermore, this method enabled a comprehensive Genome-wide association study (GWAS) in a 293-accession genetic population. The GWAS identified 200 and 65 statistically significant single nucleotide polymorphisms (SNPs), which were tightly associated with 28 and 11 candidate genes, at the late bolting and flowering stages, respectively. These findings demonstrated that the proposed method is promising for accurate estimations of IHs in oilseed rape as well as exploring the variations within the subplot, thus providing great potential for high-throughput plant phenotyping in crop breeding.

株高是一项重要的农艺性状,影响作物结构、光合作用,进而影响最终产量和种子品质。结合无人机上的数码相机和运动结构的使用,实现了高通量作物冠层高度估计。然而,以往的研究主要集中在样地高度的预测上,忽略了对单株植物的精确估计。本研究旨在探索基于掩模区域的卷积神经网络(mask - rcnn)的无人机RGB图像在油菜不同生育期个体水平身高(IH)高通量表型分析中的潜力。在无人机飞行后,通过人工测量获得150个子样地中每个子样地9个样地的实测高度。提出了一种基于Mask-RCNN模型的数据增强油菜实例分割模型。然后利用his获得基于个人水平高度(phh)的样地高度。结果表明,在高、低杂草压力下,Mask-RCNN的F1分数分别提高了60.8%和26.6%,优于传统的Otsu方法。经过数据增强训练后的模型能够基于过曝光和欠曝光的无人机图像准确估计作物高度,表明该模型在实际场景中的适用性。PHIH预测的决定系数(r2)为0.992,均方根误差(RMSE)为4.03 cm,相对均方根误差(rRMSE)为7.68%,优于文献报道的结果,特别是在抽苔后期。该方法可预测油籽各生育期的his, r2为0.983,RMSE为2.60 cm, rRMSE为7.14%。此外,该方法能够在293个遗传群体中进行全面的全基因组关联研究(GWAS)。GWAS在抽穗期和开花期分别鉴定出200个和65个具有统计学意义的单核苷酸多态性(snp),分别与28个和11个候选基因密切相关。这些结果表明,该方法有望准确估计油菜的his,并探索亚区内的变化,从而为作物育种中的高通量植物表型分析提供了巨大的潜力。
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引用次数: 0
Extracting illuminated vegetation, shadowed vegetation and background for finer fractional vegetation cover with polarization information and a convolutional network 利用偏振信息和卷积网络提取照明植被、阴影植被和背景,以获得更精细的植被覆盖分数
IF 6.2 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-13 DOI: 10.1007/s11119-023-10094-w
Hongru Bi, Wei Chen, Yi Yang

Shadows are inevitable in vegetated remote sensing scenes due to variations in viewing and solar geometries, resulting in illuminated vegetation, shadowed vegetation, illuminated background and shadowed background. In RGB images, shadowed vegetation is difficult to separate from the shadowed background because their spectra are very similar in the visible light range. Furthermore, shadowed vegetation may provide different ecological functions than illuminated vegetation. Therefore, it is important to extract both illuminated and shadowed vegetation instead of combining them into one vegetation class. However, most previous studies focused on extracting total vegetation cover and neglected separating illuminated and shadowed vegetation, partly due to a lack of sufficient information. In this study, polarization information is introduced to extract illuminated vegetation, shadowed vegetation and background simultaneously with different deep learning algorithms. The experimental results show that the addition of polarization information can effectively improve the extraction accuracy of illuminated vegetation, shadowed vegetation and background, with a maximum accuracy improvement of 12.2%. The accuracy of shadow vegetation improved the most, with a rate of 21.8%. The results of this study suggest that by adding polarization information, illuminated and shadowed vegetation can be accurately extracted to provide a reliable vegetation cover product for remote sensing.

在植被遥感场景中,由于视角和太阳几何形状的变化,不可避免地会出现阴影,从而产生受光植被、阴影植被、受光背景和阴影背景。在 RGB 图像中,阴影植被很难从阴影背景中分离出来,因为它们在可见光范围内的光谱非常相似。此外,阴影植被与光照植被可能具有不同的生态功能。因此,必须同时提取照明植被和阴影植被,而不是将它们合并为一类植被。然而,以往的研究大多侧重于提取植被总覆盖率,而忽略了分离受光照植被和阴影植被,部分原因是缺乏足够的信息。本研究引入了偏振信息,利用不同的深度学习算法同时提取照明植被、阴影植被和背景。实验结果表明,偏振信息的加入能有效提高光照植被、阴影植被和背景的提取精度,最高精度提高了 12.2%。其中,阴影植被的精度提高幅度最大,达到 21.8%。该研究结果表明,通过添加偏振信息,可以准确提取照明植被和阴影植被,为遥感提供可靠的植被覆盖产品。
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引用次数: 0
期刊
Precision Agriculture
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