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Image Based-Phenotyping and Selection Index Based on Multivariate Analysis for Rice Hydroponic Screening under Drought Stress 干旱胁迫下水稻水培筛选的图像表型和多元选择指数
Q3 Agricultural and Biological Sciences Pub Date : 2021-12-01 DOI: 10.9787/pbb.2021.9.4.272
A. A. Laraswati, R. Padjung, M. Farid, N. Nasaruddin, M. Anshori, A. Nur, A. I. Sakinah
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引用次数: 5
Evaluation of Bentazone Reaction by Growth Stage and Bentazone Dose Across Soybean Genotypes 不同大豆基因型间苯他酮的生长阶段和剂量评价
Q3 Agricultural and Biological Sciences Pub Date : 2021-12-01 DOI: 10.9787/pbb.2021.9.4.331
L. Ali, Hyun-Jeong Jo, J. Song, Jeong-Dong Lee
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引用次数: 3
Fine-Mapping of a Major Quantitative Trait Locus q2ID1 for Rice Stem Diameter 水稻茎径主要数量性状q2ID1位点的精细定位
Q3 Agricultural and Biological Sciences Pub Date : 2021-12-01 DOI: 10.9787/pbb.2021.9.4.298
Ye-Ji Lee, Y. Yu, Hyeonso Ji, G. Lee, N. Hyung, Keunpyo Lee, Tae-Ho Kim
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引用次数: 0
Evaluation of the drought resistance of pea (Pisum sativum L.) from the VIR collection VIR品种豌豆(Pisum sativum L.)抗旱性评价
Q3 Agricultural and Biological Sciences Pub Date : 2021-10-15 DOI: 10.30901/2658-6266-2021-2-o1
E. Semenova, I. Kosareva
Background. As a result of global warming, climate change is now taking place, increasing the frequency and duration of droughts. Therefore, the development of new varieties with an increased drought resistance and adaptation to certain environmental conditions is of primary importance. The aim of this work was to modify the express method of drought resistance evaluation in peas at an early stage of plant growth and development, and to apply this method to test 50 accessions from the VIR global collection.Materials and methods. Drought resistance studies involved garden pea accessions of different eco-geographic origin, which had been previously characterized in field tests in conditions of the Krasnodar Territory in 2017-2019. The roll-ups protocol was used for evaluating early drought resistance in the accessions. The stress intensity was modified by varying the concentration of sucrose in the solution used for growing of pea seedlings. To select an appropriate concentration, an osmotic pressure of 0.5 and 0.7 MPa was applied. As a result, the osmotic pressure of 0.5 MPa was chosen. The diagnostic criterion of the method is the radicle length index (RLI), that is, the ratio of the average radicle length of seedlings against a provocative background to the control values.Results. The osmotically active solution led to significant reduction in the radicle length of pea seedlings. The studied accessions exhibited considerable genetic variability for early drought tolerance, the RLI value varied from 0.28 to 0.88. Sources of high drought resistance during the period of seedling growth have been identified. The correlation analysis showed the absence of a reliable relationship between the RLI and the crop structure indicators (correlation coefficients from r = +0.17 to r = -0.24).Conclusion. By using the method of determining the relative drought tolerance at early stages of pea accessions development, one highly resistant (k-9333 from Morocco) and 10 resistant accessions (k-1495, k-9372, k-9401, k-9418, k-9733, k-9909, k-9934, k-9938, k-10072, and k-10116.) have been identified.
背景。由于全球变暖,气候正在发生变化,增加了干旱的频率和持续时间。因此,培育抗旱性强、适应特定环境条件的新品种至关重要。本工作的目的是修改植物生长发育早期豌豆抗旱性评价的表达方法,并将该方法应用于VIR全球收集的50个品种。材料和方法。抗旱性研究涉及不同生态地理来源的园豌豆品种,这些品种此前已于2017-2019年在克拉斯诺达尔地区的野外试验中进行了鉴定。采用卷取方案评价了材料的早期抗旱性。通过改变豌豆幼苗生长溶液中蔗糖的浓度来调节胁迫强度。为选择合适的浓度,分别施加0.5和0.7 MPa的渗透压。因此,选择渗透压为0.5 MPa。该方法的诊断标准是胚根长度指数(RLI),即在刺激背景下幼苗的平均胚根长度与控制值的比值。渗透活性溶液显著降低了豌豆幼苗的根长。研究材料的早期抗旱性表现出较大的遗传变异,RLI值在0.28 ~ 0.88之间。在幼苗生长期间,已经确定了高抗旱性的来源。相关分析显示,RLI与作物结构指标之间不存在可靠的相关关系(相关系数r = +0.17 ~ -0.24)。采用相对耐旱性测定方法,鉴定了1个高抗旱性品种(摩洛哥产k-9333)和10个抗旱性品种(k-1495、k-9372、k-9401、k-9418、k-9733、k-9909、k-9934、k-9938、k-10072和k-10116)。
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引用次数: 0
Diagnostic value of Rf1 gene molecular markers in sunflower 向日葵Rf1基因分子标记的诊断价值
Q3 Agricultural and Biological Sciences Pub Date : 2021-10-15 DOI: 10.30901/2658-6266-2021-2-o3
I. Anisimova, Yu.I. Karabitsina, N. Alpatieva, E. B. Kusnetsova, N. V. Titov, A. Y. Lyutko, V. Gavrilova
Background. Modern production of sunflower seeds is currently based on the cultivation of high-yielding heterotic F1 hybrids from cross-breeding of lines with cytoplasmic male sterility (CMS) of PET1-type and fertility restorer lines. The paternal parent serves as a donor of the nuclear Rf1 gene functional allele, which is responsible for pollen fertility restoration in F1 plants. The detection of carriers of the Rf1 locus recessive and dominant alleles using diagnostic molecular markers accelerates breeding of female and male parental lines for creating hybrids. Materials and methods. The material for the study included 75 lines of various origins from the VIR sunflower genetic collection as well as hybrids from crosses of VIR 116A sterile line with fertile lines differing in the type of cytoplasm (fertile or sterile) and the presence of molecular markers, most of which were linked to the Rf1 locus. For marker validation, two different approaches were used: either by analyzing associations between the ability of a line to restore pollen fertility and the presence of molecular markers in its genotype, or by estimating recombination frequency between the Rf1 locus and marker loci in four segregating hybrid populations. Results. According to the obtained results, no markers demonstrated 100% efficiency in the analysis of the sample of genotypes. The ORS511 marker was most frequently observed among the lines presumably carrying the dominant allele Rf1. Pollen fertility of F1 hybrids from interline crossings was 89-99%. The segregation for fertility/sterility in F2 fitted the theoretical ratio of 3:1 expected in case of the monogenic control of the trait. The markers HRG01, HRG02 and ORS511 were linked to the fertility restoration trait, with recombination rates between Rf1 locus and markers varying in different cross combinations. The analysis of VIR 116А × VIR 740 and VIR 116А × RIL 130 hybrids showed that among the marker loci studied, the ORS511 was closest to the Rf1 locus Rf1 (recombination frequency of 2.2 and 3.3%, respectively). The recombination rate between the Rf1 and ORS511 loci equaled 7.5% in the cross VIR 116А × VIR 210 and 8.9% in VIR 116 × VIR 195. Conclusion. The markers ORS511, HRG01 and HRG02 are the most efficient for the identification of alleles of the Rf1 gene and for the marker assisted selection in hybrid populations produced involving sunflower lines from the VIR collection.
背景。现代葵花籽生产目前是以pet1型细胞质雄性不育系与育性恢复系杂交培育高产杂种F1为基础的。父本作为核Rf1基因功能等位基因的供体,负责F1植物花粉育性恢复。利用诊断分子标记检测Rf1基因座隐性和显性等位基因的携带者,加快了雌、雄亲本系的杂交育种。材料和方法。该研究的材料包括来自VIR向日葵遗传收集的75个不同来源的系,以及来自VIR 116A不育系的杂交杂交种,这些不育系在细胞质类型(可育或不育)和分子标记的存在方面存在差异,其中大多数与Rf1位点相关。为了进行标记验证,使用了两种不同的方法:通过分析一个品系恢复花粉育性的能力与其基因型中分子标记的存在之间的关系,或者通过估计四个分离杂交群体中Rf1位点和标记位点之间的重组频率。结果。根据获得的结果,没有任何标记在基因型样本分析中表现出100%的效率。ORS511标记在可能携带显性等位基因Rf1的系中最常见。杂种花粉育性为89 ~ 99%。F2的育性/不育性分离符合该性状单基因控制条件下3:1的理论比率。标记HRG01、HRG02和ORS511与育性恢复性状相关,不同杂交组合中Rf1位点与标记的重组率不同。对VIR 116А × VIR 740和VIR 116А × RIL 130的分析表明,在研究的标记位点中,ORS511最接近Rf1位点Rf1(重组频率分别为2.2和3.3%)。Rf1位点与ORS511位点的重组率在VIR 116А × VIR 210杂交中为7.5%,在VIR 116 × VIR 195杂交中为8.9%。结论。标记ORS511、HRG01和HRG02是鉴定Rf1基因等位基因和在涉及VIR收集的向日葵系的杂交群体中进行标记辅助选择的最有效标记。
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引用次数: 3
Identification of leaf rust resistance genes in the new Russian varieties of common wheat 俄罗斯普通小麦新品种抗叶锈病基因的鉴定
Q3 Agricultural and Biological Sciences Pub Date : 2021-10-15 DOI: 10.30901/2658-6266-2021-2-o2
E. Gultyaeva, E. Shaydayuk
Background. Wheat leaf rust caused by Puccinia triticina Erikss. is a significant wheat disease in all regions of the Russian Federation. The genetic diversity of the cultivated wheat varieties regarding the type of resistance and genes that control it ensures reliable protection of this crop against the pathogen. The aim of this work was to characterize the diversity of new Russian varieties of winter and spring common wheat for leaf rust resistance genes (Lr-genes).Materials and Methods. The research material was represented by 43 varieties of winter and 25 of spring wheat included in the State Register of Selection Achievements of the Russian Federation in 2018-2020.Results. Using molecular markers, 18 Lr genes were identified: Lr1, Lr3, Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr28, Lr29, Lr34, Lr35, Lr37, Lr41 (39), Lr47 and Lr66. A phytopathological test was used to clarify the results of molecular analysis. Ninety-three percent of the studied wheat varieties were found to contain Lr genes, either separately or in combinations. These were the highly and partially effective genes Lr24, Lr9, and Lr19, adult plant resistance genes Lr34 and Lr37, and ineffective genes Lr1, Lr3, Lr10, Lr20, and Lr26. The Lr24 gene has been identified for the first time in Russian varieties. The spring variety ‘Leader 80’, harboring this gene, is recommended for cultivation in the West Siberian and East Siberian regions. An effective combination of Lr9 + Lr26 genes, individually overcome by the pathogen, was determined in the spring cultivar ‘Silach’, highly resistant to leaf rust. The Lr9 gene was found in the winter variety ‘Gerda’, which is recommended for cultivation in the North Caucasus region. Previously, the varieties with Lr9 were not grown in the North Caucasus. An increase in the number of leaf rust resistant accessions protected by the effective adult plant resistance gene Lr37 is noted among wheat varieties undergoing regional adaptation testing. Many of the identified Lr genes (Lr19, Lr24, Lr26, Lr34, Lr37) are linked with effective Sr genes (Sr25, Sr24, Sr31, Sr57, and Sr38), which additionally ensures stable genetic protection of wheat against stem rust.Conclusions. The obtained information about representation of Lr genes in wheat varieties should be used in regional breeding programs. A timely replacement of genetically protected varieties allows stabilizing the populational composition of the phytopathogen and reducing the likelihood of epiphytotics.
背景。小麦锈菌引起的小麦叶锈病。是俄罗斯联邦所有地区的一种重要小麦病害。栽培小麦品种在抗性类型和控制它的基因方面的遗传多样性确保了这种作物对病原体的可靠保护。本研究的目的是鉴定俄罗斯冬小麦和春小麦新品种抗叶锈病基因(lr -基因)的多样性。材料与方法。该研究材料以列入2018-2020年俄罗斯联邦国家选择成果登记册的43个冬小麦品种和25个春小麦品种为代表。通过分子标记鉴定出18个Lr基因:Lr1、Lr3、Lr9、Lr10、Lr19、Lr20、Lr21、Lr24、Lr25、Lr26、Lr28、Lr29、Lr34、Lr35、Lr37、Lr41(39)、Lr47和Lr66。植物病理学检查用于澄清分子分析的结果。在所研究的小麦品种中,93%被发现含有Lr基因,无论是单独的还是组合的。其中高效和部分有效基因Lr24、Lr9和Lr19,成虫抗性基因Lr34和Lr37,无效基因Lr1、Lr3、Lr10、Lr20和Lr26。Lr24基因首次在俄罗斯品种中被鉴定出来。春季品种‘Leader 80’含有这种基因,推荐在西西伯利亚和东西伯利亚地区种植。结果表明,Lr9 + Lr26基因组合对叶锈病具有较强的抗性。在北高加索地区推荐栽培的冬季品种Gerda中发现了Lr9基因。以前,含有Lr9的品种没有在北高加索种植。在进行区域适应试验的小麦品种中,受有效成株抗叶锈病基因Lr37保护的抗叶锈病品种数量有所增加。许多已鉴定的抗病基因(Lr19、Lr24、Lr26、Lr34、Lr37)与有效抗病基因(Sr25、Sr24、Sr31、Sr57和Sr38)互联,为小麦抗茎锈病提供了稳定的遗传保护。所获得的Lr基因在小麦品种中的代表性信息应用于区域育种规划。及时更换受遗传保护的品种可以稳定植物病原体的种群组成,减少附生植物的可能性。
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引用次数: 3
SSR analysis of modern Russian potato varieties using DNA samples of nomenclatural standards 利用命名标准DNA样本对现代俄罗斯马铃薯品种进行SSR分析
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-02 DOI: 10.30901/2658-6266-2020-4-o2
O. Antonova, N. Klimenko, D. A. Rybakov, N. A. Fomina, V. V. Zheltova, L. Novikova, T. Gavrilenko
The N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) is developing new approaches to documentation of national cultivars, taking into account recommendations of the International Code of Nomenclature for Cultivated Plants in parallel with methods of genetic certification. The nomenclatural standard of a particular cultivar represented by a herbarium specimen can be used as a reference for verifying authenticity and uniformity of cultivar specimens obtained from various sources. The verification requires fast and reliable methods for cultivar genotyping. This paper presents protocols for modified methods of DNA extraction, PCR-analysis and SSR-genotyping, which allow potato cultivars identification without the use of expensive reagent kits. A set of ten chromosome-specific microsatellite markers was used to study polymorphisms in 66 modern Russian potato cultivars, as well as in 11 pre-cultivars and breeding clones, represented by nomenclatural standards and voucher specimens, respectively. This subset of 77 specimens has demonstrated a high level of polymorphism in ten studied microsatellite loci. The SSR analysis identified 73 alleles; 7.3 alleles per locus were observed on average, the number of which varied from 3 (STG0025 locus) to 11 (locus StI046). The PIC values varied from 0.544 (STG0025 locus) to 0.836 (StI046 locus). The alleles, unique for this subset, were found at six studied loci. The high level of polymorphism at the SSR loci made it possible to unambiguously identify almost every cultivar, with the exception of the expected coincidence of microsatellite profiles of two cultivars, which are somaclonal variants. Using an optimized set of eight microsatellite markers, the genetic relationships of modern Russian potato cultivars were studied.
瓦维洛夫全俄植物遗传资源研究所(VIR)考虑到《国际栽培植物命名法》的建议和遗传认证方法,正在开发记录国家品种的新方法。植物标本馆标本所代表的特定品种的命名标准,可作为检验从不同来源获得的品种标本的真实性和一致性的参考。验证需要快速可靠的品种基因分型方法。本文介绍了改进的DNA提取、pcr分析和ssr基因分型方法,使马铃薯品种鉴定无需使用昂贵的试剂盒。利用10个染色体特异性微卫星标记,对66个现代俄罗斯马铃薯品种、11个预栽培品种和育种无性系(分别以命名标准和代用样品为代表)的多态性进行了研究。这77个样本的子集在10个研究的微卫星位点中显示出高水平的多态性。SSR分析共鉴定出73个等位基因;平均每个位点检测到7.3个等位基因,等位基因数从3个(STG0025)到11个(StI046)不等。PIC值为0.544 (STG0025位点)~ 0.836 (StI046位点)。在6个研究位点上发现了该亚群特有的等位基因。SSR位点的高水平多态性使得几乎所有品种都可以明确地识别,除了两个品种的微卫星谱是体细胞无性系变异。利用优化后的8个微卫星标记,对俄罗斯现代马铃薯品种的亲缘关系进行了研究。
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引用次数: 8
Development of a Simple Enzymatic Method for Screening Sucrose Content in Legume Seeds 酶法筛选豆科种子中蔗糖含量的研究
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-01 DOI: 10.9787/PBB.2021.9.3.250
Gyutae Kim, Aron Park, W. Kim, C. Moon, B. Kang, Seonghyeon Kim, Yu-mi Choi, B. Ha
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引用次数: 0
OsGRAS19 and OsGRAS32 Control Tiller Development in Rice OsGRAS19和OsGRAS32控制水稻分蘖发育
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-01 DOI: 10.9787/PBB.2021.9.3.239
Jinwon Lee, Jinmi Yoon, Seulbi Lee, G. An, Soon-Ki Park
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引用次数: 0
Screening and Breeding for Biofortification of Rice with Protein and High Lysine Contents 高蛋白高赖氨酸生物强化水稻的筛选与选育
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-01 DOI: 10.9787/PBB.2021.9.3.199
Ji-Yun Lee, Ju-Won Kang, Su-min Jo, Youngho Kwon, So-Myeong Lee, Sais-Beul Lee, Dongjin Shin, Dong-Soo Park, Jong-Hee Lee, J. Ko, Jun-Hyeon Cho
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引用次数: 4
期刊
Plant breeding and biotechnology
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