Pub Date : 2021-12-01DOI: 10.9787/pbb.2021.9.4.272
A. A. Laraswati, R. Padjung, M. Farid, N. Nasaruddin, M. Anshori, A. Nur, A. I. Sakinah
{"title":"Image Based-Phenotyping and Selection Index Based on Multivariate Analysis for Rice Hydroponic Screening under Drought Stress","authors":"A. A. Laraswati, R. Padjung, M. Farid, N. Nasaruddin, M. Anshori, A. Nur, A. I. Sakinah","doi":"10.9787/pbb.2021.9.4.272","DOIUrl":"https://doi.org/10.9787/pbb.2021.9.4.272","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49171986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.9787/pbb.2021.9.4.331
L. Ali, Hyun-Jeong Jo, J. Song, Jeong-Dong Lee
{"title":"Evaluation of Bentazone Reaction by Growth Stage and Bentazone Dose Across Soybean Genotypes","authors":"L. Ali, Hyun-Jeong Jo, J. Song, Jeong-Dong Lee","doi":"10.9787/pbb.2021.9.4.331","DOIUrl":"https://doi.org/10.9787/pbb.2021.9.4.331","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48808653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.9787/pbb.2021.9.4.298
Ye-Ji Lee, Y. Yu, Hyeonso Ji, G. Lee, N. Hyung, Keunpyo Lee, Tae-Ho Kim
{"title":"Fine-Mapping of a Major Quantitative Trait Locus q2ID1 for Rice Stem Diameter","authors":"Ye-Ji Lee, Y. Yu, Hyeonso Ji, G. Lee, N. Hyung, Keunpyo Lee, Tae-Ho Kim","doi":"10.9787/pbb.2021.9.4.298","DOIUrl":"https://doi.org/10.9787/pbb.2021.9.4.298","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45303295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-15DOI: 10.30901/2658-6266-2021-2-o1
E. Semenova, I. Kosareva
Background. As a result of global warming, climate change is now taking place, increasing the frequency and duration of droughts. Therefore, the development of new varieties with an increased drought resistance and adaptation to certain environmental conditions is of primary importance. The aim of this work was to modify the express method of drought resistance evaluation in peas at an early stage of plant growth and development, and to apply this method to test 50 accessions from the VIR global collection.Materials and methods. Drought resistance studies involved garden pea accessions of different eco-geographic origin, which had been previously characterized in field tests in conditions of the Krasnodar Territory in 2017-2019. The roll-ups protocol was used for evaluating early drought resistance in the accessions. The stress intensity was modified by varying the concentration of sucrose in the solution used for growing of pea seedlings. To select an appropriate concentration, an osmotic pressure of 0.5 and 0.7 MPa was applied. As a result, the osmotic pressure of 0.5 MPa was chosen. The diagnostic criterion of the method is the radicle length index (RLI), that is, the ratio of the average radicle length of seedlings against a provocative background to the control values.Results. The osmotically active solution led to significant reduction in the radicle length of pea seedlings. The studied accessions exhibited considerable genetic variability for early drought tolerance, the RLI value varied from 0.28 to 0.88. Sources of high drought resistance during the period of seedling growth have been identified. The correlation analysis showed the absence of a reliable relationship between the RLI and the crop structure indicators (correlation coefficients from r = +0.17 to r = -0.24).Conclusion. By using the method of determining the relative drought tolerance at early stages of pea accessions development, one highly resistant (k-9333 from Morocco) and 10 resistant accessions (k-1495, k-9372, k-9401, k-9418, k-9733, k-9909, k-9934, k-9938, k-10072, and k-10116.) have been identified.
{"title":"Evaluation of the drought resistance of pea (Pisum sativum L.) from the VIR collection","authors":"E. Semenova, I. Kosareva","doi":"10.30901/2658-6266-2021-2-o1","DOIUrl":"https://doi.org/10.30901/2658-6266-2021-2-o1","url":null,"abstract":"Background. As a result of global warming, climate change is now taking place, increasing the frequency and duration of droughts. Therefore, the development of new varieties with an increased drought resistance and adaptation to certain environmental conditions is of primary importance. The aim of this work was to modify the express method of drought resistance evaluation in peas at an early stage of plant growth and development, and to apply this method to test 50 accessions from the VIR global collection.Materials and methods. Drought resistance studies involved garden pea accessions of different eco-geographic origin, which had been previously characterized in field tests in conditions of the Krasnodar Territory in 2017-2019. The roll-ups protocol was used for evaluating early drought resistance in the accessions. The stress intensity was modified by varying the concentration of sucrose in the solution used for growing of pea seedlings. To select an appropriate concentration, an osmotic pressure of 0.5 and 0.7 MPa was applied. As a result, the osmotic pressure of 0.5 MPa was chosen. The diagnostic criterion of the method is the radicle length index (RLI), that is, the ratio of the average radicle length of seedlings against a provocative background to the control values.Results. The osmotically active solution led to significant reduction in the radicle length of pea seedlings. The studied accessions exhibited considerable genetic variability for early drought tolerance, the RLI value varied from 0.28 to 0.88. Sources of high drought resistance during the period of seedling growth have been identified. The correlation analysis showed the absence of a reliable relationship between the RLI and the crop structure indicators (correlation coefficients from r = +0.17 to r = -0.24).Conclusion. By using the method of determining the relative drought tolerance at early stages of pea accessions development, one highly resistant (k-9333 from Morocco) and 10 resistant accessions (k-1495, k-9372, k-9401, k-9418, k-9733, k-9909, k-9934, k-9938, k-10072, and k-10116.) have been identified.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"609 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78958444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-15DOI: 10.30901/2658-6266-2021-2-o3
I. Anisimova, Yu.I. Karabitsina, N. Alpatieva, E. B. Kusnetsova, N. V. Titov, A. Y. Lyutko, V. Gavrilova
Background. Modern production of sunflower seeds is currently based on the cultivation of high-yielding heterotic F1 hybrids from cross-breeding of lines with cytoplasmic male sterility (CMS) of PET1-type and fertility restorer lines. The paternal parent serves as a donor of the nuclear Rf1 gene functional allele, which is responsible for pollen fertility restoration in F1 plants. The detection of carriers of the Rf1 locus recessive and dominant alleles using diagnostic molecular markers accelerates breeding of female and male parental lines for creating hybrids. Materials and methods. The material for the study included 75 lines of various origins from the VIR sunflower genetic collection as well as hybrids from crosses of VIR 116A sterile line with fertile lines differing in the type of cytoplasm (fertile or sterile) and the presence of molecular markers, most of which were linked to the Rf1 locus. For marker validation, two different approaches were used: either by analyzing associations between the ability of a line to restore pollen fertility and the presence of molecular markers in its genotype, or by estimating recombination frequency between the Rf1 locus and marker loci in four segregating hybrid populations. Results. According to the obtained results, no markers demonstrated 100% efficiency in the analysis of the sample of genotypes. The ORS511 marker was most frequently observed among the lines presumably carrying the dominant allele Rf1. Pollen fertility of F1 hybrids from interline crossings was 89-99%. The segregation for fertility/sterility in F2 fitted the theoretical ratio of 3:1 expected in case of the monogenic control of the trait. The markers HRG01, HRG02 and ORS511 were linked to the fertility restoration trait, with recombination rates between Rf1 locus and markers varying in different cross combinations. The analysis of VIR 116А × VIR 740 and VIR 116А × RIL 130 hybrids showed that among the marker loci studied, the ORS511 was closest to the Rf1 locus Rf1 (recombination frequency of 2.2 and 3.3%, respectively). The recombination rate between the Rf1 and ORS511 loci equaled 7.5% in the cross VIR 116А × VIR 210 and 8.9% in VIR 116 × VIR 195. Conclusion. The markers ORS511, HRG01 and HRG02 are the most efficient for the identification of alleles of the Rf1 gene and for the marker assisted selection in hybrid populations produced involving sunflower lines from the VIR collection.
背景。现代葵花籽生产目前是以pet1型细胞质雄性不育系与育性恢复系杂交培育高产杂种F1为基础的。父本作为核Rf1基因功能等位基因的供体,负责F1植物花粉育性恢复。利用诊断分子标记检测Rf1基因座隐性和显性等位基因的携带者,加快了雌、雄亲本系的杂交育种。材料和方法。该研究的材料包括来自VIR向日葵遗传收集的75个不同来源的系,以及来自VIR 116A不育系的杂交杂交种,这些不育系在细胞质类型(可育或不育)和分子标记的存在方面存在差异,其中大多数与Rf1位点相关。为了进行标记验证,使用了两种不同的方法:通过分析一个品系恢复花粉育性的能力与其基因型中分子标记的存在之间的关系,或者通过估计四个分离杂交群体中Rf1位点和标记位点之间的重组频率。结果。根据获得的结果,没有任何标记在基因型样本分析中表现出100%的效率。ORS511标记在可能携带显性等位基因Rf1的系中最常见。杂种花粉育性为89 ~ 99%。F2的育性/不育性分离符合该性状单基因控制条件下3:1的理论比率。标记HRG01、HRG02和ORS511与育性恢复性状相关,不同杂交组合中Rf1位点与标记的重组率不同。对VIR 116А × VIR 740和VIR 116А × RIL 130的分析表明,在研究的标记位点中,ORS511最接近Rf1位点Rf1(重组频率分别为2.2和3.3%)。Rf1位点与ORS511位点的重组率在VIR 116А × VIR 210杂交中为7.5%,在VIR 116 × VIR 195杂交中为8.9%。结论。标记ORS511、HRG01和HRG02是鉴定Rf1基因等位基因和在涉及VIR收集的向日葵系的杂交群体中进行标记辅助选择的最有效标记。
{"title":"Diagnostic value of Rf1 gene molecular markers in sunflower","authors":"I. Anisimova, Yu.I. Karabitsina, N. Alpatieva, E. B. Kusnetsova, N. V. Titov, A. Y. Lyutko, V. Gavrilova","doi":"10.30901/2658-6266-2021-2-o3","DOIUrl":"https://doi.org/10.30901/2658-6266-2021-2-o3","url":null,"abstract":"Background. Modern production of sunflower seeds is currently based on the cultivation of high-yielding heterotic F1 hybrids from cross-breeding of lines with cytoplasmic male sterility (CMS) of PET1-type and fertility restorer lines. The paternal parent serves as a donor of the nuclear Rf1 gene functional allele, which is responsible for pollen fertility restoration in F1 plants. The detection of carriers of the Rf1 locus recessive and dominant alleles using diagnostic molecular markers accelerates breeding of female and male parental lines for creating hybrids. Materials and methods. The material for the study included 75 lines of various origins from the VIR sunflower genetic collection as well as hybrids from crosses of VIR 116A sterile line with fertile lines differing in the type of cytoplasm (fertile or sterile) and the presence of molecular markers, most of which were linked to the Rf1 locus. For marker validation, two different approaches were used: either by analyzing associations between the ability of a line to restore pollen fertility and the presence of molecular markers in its genotype, or by estimating recombination frequency between the Rf1 locus and marker loci in four segregating hybrid populations. Results. According to the obtained results, no markers demonstrated 100% efficiency in the analysis of the sample of genotypes. The ORS511 marker was most frequently observed among the lines presumably carrying the dominant allele Rf1. Pollen fertility of F1 hybrids from interline crossings was 89-99%. The segregation for fertility/sterility in F2 fitted the theoretical ratio of 3:1 expected in case of the monogenic control of the trait. The markers HRG01, HRG02 and ORS511 were linked to the fertility restoration trait, with recombination rates between Rf1 locus and markers varying in different cross combinations. The analysis of VIR 116А × VIR 740 and VIR 116А × RIL 130 hybrids showed that among the marker loci studied, the ORS511 was closest to the Rf1 locus Rf1 (recombination frequency of 2.2 and 3.3%, respectively). The recombination rate between the Rf1 and ORS511 loci equaled 7.5% in the cross VIR 116А × VIR 210 and 8.9% in VIR 116 × VIR 195. Conclusion. The markers ORS511, HRG01 and HRG02 are the most efficient for the identification of alleles of the Rf1 gene and for the marker assisted selection in hybrid populations produced involving sunflower lines from the VIR collection.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87073066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-15DOI: 10.30901/2658-6266-2021-2-o2
E. Gultyaeva, E. Shaydayuk
Background. Wheat leaf rust caused by Puccinia triticina Erikss. is a significant wheat disease in all regions of the Russian Federation. The genetic diversity of the cultivated wheat varieties regarding the type of resistance and genes that control it ensures reliable protection of this crop against the pathogen. The aim of this work was to characterize the diversity of new Russian varieties of winter and spring common wheat for leaf rust resistance genes (Lr-genes).Materials and Methods. The research material was represented by 43 varieties of winter and 25 of spring wheat included in the State Register of Selection Achievements of the Russian Federation in 2018-2020.Results. Using molecular markers, 18 Lr genes were identified: Lr1, Lr3, Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr28, Lr29, Lr34, Lr35, Lr37, Lr41 (39), Lr47 and Lr66. A phytopathological test was used to clarify the results of molecular analysis. Ninety-three percent of the studied wheat varieties were found to contain Lr genes, either separately or in combinations. These were the highly and partially effective genes Lr24, Lr9, and Lr19, adult plant resistance genes Lr34 and Lr37, and ineffective genes Lr1, Lr3, Lr10, Lr20, and Lr26. The Lr24 gene has been identified for the first time in Russian varieties. The spring variety ‘Leader 80’, harboring this gene, is recommended for cultivation in the West Siberian and East Siberian regions. An effective combination of Lr9 + Lr26 genes, individually overcome by the pathogen, was determined in the spring cultivar ‘Silach’, highly resistant to leaf rust. The Lr9 gene was found in the winter variety ‘Gerda’, which is recommended for cultivation in the North Caucasus region. Previously, the varieties with Lr9 were not grown in the North Caucasus. An increase in the number of leaf rust resistant accessions protected by the effective adult plant resistance gene Lr37 is noted among wheat varieties undergoing regional adaptation testing. Many of the identified Lr genes (Lr19, Lr24, Lr26, Lr34, Lr37) are linked with effective Sr genes (Sr25, Sr24, Sr31, Sr57, and Sr38), which additionally ensures stable genetic protection of wheat against stem rust.Conclusions. The obtained information about representation of Lr genes in wheat varieties should be used in regional breeding programs. A timely replacement of genetically protected varieties allows stabilizing the populational composition of the phytopathogen and reducing the likelihood of epiphytotics.
{"title":"Identification of leaf rust resistance genes in the new Russian varieties of common wheat","authors":"E. Gultyaeva, E. Shaydayuk","doi":"10.30901/2658-6266-2021-2-o2","DOIUrl":"https://doi.org/10.30901/2658-6266-2021-2-o2","url":null,"abstract":"Background. Wheat leaf rust caused by Puccinia triticina Erikss. is a significant wheat disease in all regions of the Russian Federation. The genetic diversity of the cultivated wheat varieties regarding the type of resistance and genes that control it ensures reliable protection of this crop against the pathogen. The aim of this work was to characterize the diversity of new Russian varieties of winter and spring common wheat for leaf rust resistance genes (Lr-genes).Materials and Methods. The research material was represented by 43 varieties of winter and 25 of spring wheat included in the State Register of Selection Achievements of the Russian Federation in 2018-2020.Results. Using molecular markers, 18 Lr genes were identified: Lr1, Lr3, Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr28, Lr29, Lr34, Lr35, Lr37, Lr41 (39), Lr47 and Lr66. A phytopathological test was used to clarify the results of molecular analysis. Ninety-three percent of the studied wheat varieties were found to contain Lr genes, either separately or in combinations. These were the highly and partially effective genes Lr24, Lr9, and Lr19, adult plant resistance genes Lr34 and Lr37, and ineffective genes Lr1, Lr3, Lr10, Lr20, and Lr26. The Lr24 gene has been identified for the first time in Russian varieties. The spring variety ‘Leader 80’, harboring this gene, is recommended for cultivation in the West Siberian and East Siberian regions. An effective combination of Lr9 + Lr26 genes, individually overcome by the pathogen, was determined in the spring cultivar ‘Silach’, highly resistant to leaf rust. The Lr9 gene was found in the winter variety ‘Gerda’, which is recommended for cultivation in the North Caucasus region. Previously, the varieties with Lr9 were not grown in the North Caucasus. An increase in the number of leaf rust resistant accessions protected by the effective adult plant resistance gene Lr37 is noted among wheat varieties undergoing regional adaptation testing. Many of the identified Lr genes (Lr19, Lr24, Lr26, Lr34, Lr37) are linked with effective Sr genes (Sr25, Sr24, Sr31, Sr57, and Sr38), which additionally ensures stable genetic protection of wheat against stem rust.Conclusions. The obtained information about representation of Lr genes in wheat varieties should be used in regional breeding programs. A timely replacement of genetically protected varieties allows stabilizing the populational composition of the phytopathogen and reducing the likelihood of epiphytotics.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73370671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-02DOI: 10.30901/2658-6266-2020-4-o2
O. Antonova, N. Klimenko, D. A. Rybakov, N. A. Fomina, V. V. Zheltova, L. Novikova, T. Gavrilenko
The N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) is developing new approaches to documentation of national cultivars, taking into account recommendations of the International Code of Nomenclature for Cultivated Plants in parallel with methods of genetic certification. The nomenclatural standard of a particular cultivar represented by a herbarium specimen can be used as a reference for verifying authenticity and uniformity of cultivar specimens obtained from various sources. The verification requires fast and reliable methods for cultivar genotyping. This paper presents protocols for modified methods of DNA extraction, PCR-analysis and SSR-genotyping, which allow potato cultivars identification without the use of expensive reagent kits. A set of ten chromosome-specific microsatellite markers was used to study polymorphisms in 66 modern Russian potato cultivars, as well as in 11 pre-cultivars and breeding clones, represented by nomenclatural standards and voucher specimens, respectively. This subset of 77 specimens has demonstrated a high level of polymorphism in ten studied microsatellite loci. The SSR analysis identified 73 alleles; 7.3 alleles per locus were observed on average, the number of which varied from 3 (STG0025 locus) to 11 (locus StI046). The PIC values varied from 0.544 (STG0025 locus) to 0.836 (StI046 locus). The alleles, unique for this subset, were found at six studied loci. The high level of polymorphism at the SSR loci made it possible to unambiguously identify almost every cultivar, with the exception of the expected coincidence of microsatellite profiles of two cultivars, which are somaclonal variants. Using an optimized set of eight microsatellite markers, the genetic relationships of modern Russian potato cultivars were studied.
{"title":"SSR analysis of modern Russian potato varieties using DNA samples of nomenclatural standards","authors":"O. Antonova, N. Klimenko, D. A. Rybakov, N. A. Fomina, V. V. Zheltova, L. Novikova, T. Gavrilenko","doi":"10.30901/2658-6266-2020-4-o2","DOIUrl":"https://doi.org/10.30901/2658-6266-2020-4-o2","url":null,"abstract":"The N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) is developing new approaches to documentation of national cultivars, taking into account recommendations of the International Code of Nomenclature for Cultivated Plants in parallel with methods of genetic certification. The nomenclatural standard of a particular cultivar represented by a herbarium specimen can be used as a reference for verifying authenticity and uniformity of cultivar specimens obtained from various sources. The verification requires fast and reliable methods for cultivar genotyping. This paper presents protocols for modified methods of DNA extraction, PCR-analysis and SSR-genotyping, which allow potato cultivars identification without the use of expensive reagent kits. A set of ten chromosome-specific microsatellite markers was used to study polymorphisms in 66 modern Russian potato cultivars, as well as in 11 pre-cultivars and breeding clones, represented by nomenclatural standards and voucher specimens, respectively. This subset of 77 specimens has demonstrated a high level of polymorphism in ten studied microsatellite loci. The SSR analysis identified 73 alleles; 7.3 alleles per locus were observed on average, the number of which varied from 3 (STG0025 locus) to 11 (locus StI046). The PIC values varied from 0.544 (STG0025 locus) to 0.836 (StI046 locus). The alleles, unique for this subset, were found at six studied loci. The high level of polymorphism at the SSR loci made it possible to unambiguously identify almost every cultivar, with the exception of the expected coincidence of microsatellite profiles of two cultivars, which are somaclonal variants. Using an optimized set of eight microsatellite markers, the genetic relationships of modern Russian potato cultivars were studied.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"2012 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86412521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.9787/PBB.2021.9.3.250
Gyutae Kim, Aron Park, W. Kim, C. Moon, B. Kang, Seonghyeon Kim, Yu-mi Choi, B. Ha
{"title":"Development of a Simple Enzymatic Method for Screening Sucrose Content in Legume Seeds","authors":"Gyutae Kim, Aron Park, W. Kim, C. Moon, B. Kang, Seonghyeon Kim, Yu-mi Choi, B. Ha","doi":"10.9787/PBB.2021.9.3.250","DOIUrl":"https://doi.org/10.9787/PBB.2021.9.3.250","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"9 1","pages":"250-258"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45231435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.9787/PBB.2021.9.3.239
Jinwon Lee, Jinmi Yoon, Seulbi Lee, G. An, Soon-Ki Park
{"title":"OsGRAS19 and OsGRAS32 Control Tiller Development in Rice","authors":"Jinwon Lee, Jinmi Yoon, Seulbi Lee, G. An, Soon-Ki Park","doi":"10.9787/PBB.2021.9.3.239","DOIUrl":"https://doi.org/10.9787/PBB.2021.9.3.239","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"9 1","pages":"239-249"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46197966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.9787/PBB.2021.9.3.199
Ji-Yun Lee, Ju-Won Kang, Su-min Jo, Youngho Kwon, So-Myeong Lee, Sais-Beul Lee, Dongjin Shin, Dong-Soo Park, Jong-Hee Lee, J. Ko, Jun-Hyeon Cho
{"title":"Screening and Breeding for Biofortification of Rice with Protein and High Lysine Contents","authors":"Ji-Yun Lee, Ju-Won Kang, Su-min Jo, Youngho Kwon, So-Myeong Lee, Sais-Beul Lee, Dongjin Shin, Dong-Soo Park, Jong-Hee Lee, J. Ko, Jun-Hyeon Cho","doi":"10.9787/PBB.2021.9.3.199","DOIUrl":"https://doi.org/10.9787/PBB.2021.9.3.199","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"9 1","pages":"199-212"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42719085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}