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Nomenclatural standards and genetic passports of potato cultivars bred by the Omsk Agrarian Research Center 鄂木斯克农业研究中心培育的马铃薯品种的命名标准和遗传护照
Q3 Agricultural and Biological Sciences Pub Date : 2023-03-18 DOI: 10.30901/2658-6266-2022-4-o4
D. A. Rybakov, A. Cheremisin, O. Antonova, I. Chukhina, T. Gavrilenko
In accordance with the International Code of Nomenclature for Cultivated Plants, five nomenclature standards were prepared for five potato сultivars* bred in the Omsk Agrarian Research Center: ‘Alena’, ‘Bylina Sibiri’, ‘Večernij Omsk’, ‘Triumf’, ‘Hozâûška’. Genetic passport of these five cultivars were carried out according to the new integrated strategy developed at VIR. According to the strategy, the plant material donated by the author of the cultivars to the VIR Herbarium was used for molecular genotyping. Genetic passports included data of allelic composition of eight chromosome specific microsatellite loci, markers of different types of organelle DNA, as well as data about diagnostic fragments of 15 markers of 11 R-genes conferring resistance to the most dangerous diseases and pests of potato: late blight, nematodes, potato X and Y viruses. Data from the developed genetic passports of five Omsk cultivars were compared to the results of genotyping of samples of the same cultivars obtained from different sources. Based on the analysis of pedigrees and genetic passports of these five cultivars we established the origin of their resistance to harmful organisms.
根据《国际栽培植物命名法》,为鄂木斯克农业研究中心培育的五个马铃薯品种*制定了五个命名标准:“Alena”、“Bylina Sibiri”、“ve ernij鄂木斯克”、“Triumf”、“Hozâûška”。根据VIR开发的新综合策略对这5个品种进行遗传通行证。根据该策略,将作者捐赠给VIR植物标本室的植物材料用于分子基因分型。遗传护照包括8个染色体特异性微卫星位点的等位基因组成数据、不同类型的细胞器DNA标记,以及11个r基因的15个标记的诊断片段数据,这些r基因具有对马铃薯最危险的病虫害(晚疫病、线虫、马铃薯X和Y病毒)的抗性。将5个鄂木斯克品种遗传护照的数据与不同来源的同一品种样品的基因分型结果进行了比较。通过对这5个品种的家系和遗传护照的分析,确定了它们抗有害生物的来源。
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引用次数: 1
New approaches to registration and conservation of domestic cultivars of berry crops in the VIR Genebank on the example of red raspberry and black currant 以红覆盆子和黑加仑为例,在VIR基因库中登记和保护国内浆果作物品种的新方法
Q3 Agricultural and Biological Sciences Pub Date : 2023-03-08 DOI: 10.30901/2658-6266-2022-4-o5
T. Gavrilenko, S. E. Dunaeva, O. Tikhonova, I. Chukhina
A collection of nomenclatural standards is being created at VIR for domestic cultivars of various crops in accordance with the International Code of Nomenclature for Cultivated Plants (ICNCP). A new complex strategy was proposed for vegetatively propagated crops for registering domestic cultivars received from their authors in the VIR genebank. In addition to the creation of nomenclature standards, the strategy includes the development of a genetic passport of a cultivar and the use of biotechnological methods to preserve explants (buds, meristems) isolated from plant material transferred by breeders to the VIR Herbarium. This approach can be used for any vegetatively propagated crop applying a protocol developed specifically for an individual crop. For raspberry and black currant varieties, the collecting of plant material, its preparation for the registration of nomenclature standards and the preservation of viable samples under controlled in vitro conditions have specific features. This article provides detailed protocols for performing the mentioned work for raspberry and black currant varieties. In addition, the article summarizes the first results of the implementation of our proposed strategy on the example of domestic raspberry and black currant varieties created in various breeding centers of Russia. Three years of joint work of VIR researchers and breeders from four breeding centers in five regions of the country have resulted in creation of nomenclature standards for 20 raspberry varieties, as well as for five black currant varieties bred at VIR. Thirteen samples of raspberry varieties and four of black currant varieties, genetically identical to nomenclature standards, were introduced into in vitro culture; four raspberry cultivars have been placed in the VIR cryobank for the long-term cryopreservation.
VIR正在根据《国际栽培植物命名规则》为各种作物的国内品种制定一套命名标准。提出了一种新的复杂策略,使无性繁殖作物在VIR基因库中登记其作者提供的国内品种。除了制定命名标准外,该战略还包括开发一个品种的遗传通行证,并使用生物技术方法保存从育种者转移到VIR植物标本室的植物材料中分离出来的外植体(芽、分生组织)。这种方法可以用于任何无性繁殖作物,应用专门为单个作物开发的协议。对于覆盆子和黑加仑品种来说,植物材料的收集、命名标准的登记准备和体外受控条件下活菌样品的保存都有其特点。本文提供了对覆盆子和黑醋栗品种进行上述工作的详细协议。此外,本文还以俄罗斯各育种中心培育的国产覆盆子和黑醋栗品种为例,总结了实施本文提出的战略的初步结果。来自该国5个地区4个育种中心的VIR研究人员和育种人员经过3年的联合工作,已经为20个覆盆子品种以及在VIR育种的5个黑加仑品种制定了命名标准。将13个遗传上与命名标准相同的覆盆子品种和4个黑加仑品种的样品引入离体培养;4个覆盆子品种被放置在VIR cryobank进行长期冷冻保存。
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引用次数: 2
Genome-Wide Association Study of Submergence Tolerance in Rice (Oryza sativa L.) 水稻耐淹性的全基因组关联研究
Q3 Agricultural and Biological Sciences Pub Date : 2023-03-01 DOI: 10.9787/pbb.2023.11.1.25
Seong-Gyu Jang, Backki Kim, Yongchul Kim, Soon-Wook Kwon
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引用次数: 2
Prospects of Embryo Rescue in Developing Novel Brassica Genotypes 培育芸苔新基因型的胚胎拯救展望
Q3 Agricultural and Biological Sciences Pub Date : 2023-03-01 DOI: 10.9787/pbb.2023.11.1.1
Romana Sharmin Ripa, S. Jyoti, A. Robin
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引用次数: 0
Development of Speed-Breeding System for Korean Soybean Varieties [Glycine maxa (L.) Merr] Using LED Light Source 韩国大豆品种快速育种系统的开发[Glycine maxa (L.)]使用LED光源
Q3 Agricultural and Biological Sciences Pub Date : 2023-03-01 DOI: 10.9787/pbb.2023.11.1.49
Daewoong Lee, Kyu-Heung Han, Ji Hong Kim, Tae-Hwan Jun, Ju Seok Lee
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引用次数: 0
Morphological Variation in Normal Maize Landrace Accessions Collected from South Sudan 南苏丹普通玉米地方品种的形态变异
Q3 Agricultural and Biological Sciences Pub Date : 2023-03-01 DOI: 10.9787/pbb.2023.11.1.15
Emmanuel Andrea Mathiang, K. Sa, Hyeon-Jin Park, Songhoe Jang, Ju Kyong Lee
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引用次数: 1
Allelic Diversity at Protein Disulfide Isomerase Like 1-1 (PDIL1-1) Gene is Associated with Amylose Content in Japonica Rice 蛋白质二硫化物异构酶样1-1(PDIL1-1)基因等位基因多样性与粳稻直链淀粉含量的相关性
Q3 Agricultural and Biological Sciences Pub Date : 2023-03-01 DOI: 10.9787/pbb.2023.11.1.56
C. Adeva, Ju-Won Kang, Kyu-Chan Shim, Ngoc Ha Luong, Hyun-Sook Lee, Jong-Hee Lee, Sang-Nag Ahn
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引用次数: 0
In Vitro Propagation and Secondary Metabolite Production of Medicinal Plant of Euchresta horsfieldii (Lesch) Benn. 药用植物马蹄莲离体繁殖及次生代谢物生产研究。
Q3 Agricultural and Biological Sciences Pub Date : 2023-03-01 DOI: 10.9787/pbb.2023.11.1.34
Andriyana Setyawati, Samanhudi Samanhudi, W. Prameswari, D. Syukri, Defrita Fitri Ramadhani, Okky Talitha
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引用次数: 0
Callus formation ability in cultivated peanuts (Arachis hypogaea L.) 栽培花生愈伤组织形成能力的研究
Q3 Agricultural and Biological Sciences Pub Date : 2022-12-15 DOI: 10.30901/2658-6266-2022-3-o4
V. D. Bemova, L. G. Makarova, E. O. Gurina, V. Gavrilova, T. Matveeva
Background: Russia is one of the largest peanut importing countries. At the same time, in the south of the country, several zones meet the requirements for peanut cultivation. It is possible to increase the yield of the existing peanut varieties by using modern biotechnology methods, in particular agrobacterial transformation. It is known from the literature data that different peanut genotypes and explants from various sources react differently to in vitro regeneration. Successful regeneration depends on the correct protocol, including both the type of regeneration and the composition of media promoting growth and in vitro induction.Objectives: a technique for obtaining peanut regenerants in in vitro culture.Materials and methods: Eight peanut accessions from the VIR collection of different origin were used in the work. Embryonic explants were grown on Murashige-Skoog medium supplemented with the hormone 2,4-dichlorophenoxyacetic acid (2,4-D).Results and conclusions: As a result of assessing the regenerative ability of peanuts grown on Murashige-Skoog medium with the hormone 2,4-D at a concentration of 2 g/L, differences in the callus formation ability were revealed in different accessions. Those with catalog numbers k-793, k-2054 and k-2055 did not form organogenic calli, while accessions k-698 and k-1987 showed the highest percentage of callus formation from embryonic explants.
背景:俄罗斯是最大的花生进口国之一。与此同时,在该国南部,有几个地区符合花生种植的要求。利用现代生物技术方法,特别是农杆菌转化,可以提高现有花生品种的产量。从文献资料可知,不同花生基因型和不同来源的外植体对离体再生的反应不同。成功的再生取决于正确的方案,包括再生的类型和促进生长和体外诱导的培养基组成。目的:建立花生再生体离体培养技术。材料与方法:选用来自VIR不同来源的8份花生材料。胚胎外植体在添加激素2,4-二氯苯氧乙酸(2,4- d)的Murashige-Skoog培养基上生长。结果与结论:通过对花生在浓度为2 g/L的激素2,4- d的Murashige-Skoog培养基上生长的再生能力进行评价,发现不同品种的愈伤组织形成能力存在差异。目录号k-793、k-2054和k-2055的愈伤组织不形成器官源性愈伤组织,而k-698和k-1987的愈伤组织形成率最高。
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引用次数: 0
Review on Mechanism of Mineral Phosphate Solubilization in Fast-Growing Rhizobia Based on Sugar Utilization 基于糖利用的速生根瘤菌磷矿增溶机理研究进展
Q3 Agricultural and Biological Sciences Pub Date : 2022-12-01 DOI: 10.9787/pbb.2022.10.4.203
Bhumi R. Rajguru, Vaibhav Bhatt
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引用次数: 0
期刊
Plant breeding and biotechnology
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