Pub Date : 2022-04-05DOI: 10.30901/2658-6266-2022-1-o1
N. M. Zoteeva, O. Antonova, N. Klimenko, T. Gavrilenko
Background. The harmful organisms affecting potato cause great economic damage in all areas where the crop is grown. One of the most economically important pathogens is the potato late blight (agent Phytophthora infestans Mont. (de Bary)). Also, Potato Virus Y (PVY) and potato cyst nematode (PCN) of the Ro1 pathotype, the only one that occurs in the Russian Federation, cause significant yield losses. Materials and methods. Phytopathological and molecular screening was carried out on original interspecific potato hybrids obtained in 11 crossing combinations, seven of which were studied for the first time. Hybrids resulted from crosses that involved Mexican and South American potato species as sources of late blight and PVY resistance. Field observations of late blight resistance were conducted during three seasons under high infection pressure. A part of plant material was screened in laboratory tests. PCR tests employed 9 DNA markers of genes associated with resistance to Ph. infestans, PVY and PCN of Ro1 pathotype used in our previous study. Results. In field evaluation, hybrid clones mostly expressed high, and a part of them moderate late blight resistance. Of the 6 markers of the used Rpi genes, at least two were detected in most clones, while some had 3 or 4 markers. Some clones were found to have markers of PCN resistance genes and of several genes for resistance to PVY. The cytoplasm type was determined for all plant material. Conclutions. Within a small but pedigree-diverse set of interspeсific hybrids, the markers of resistance genes to all three pests were found. A part of clones obtained by using the sources of PVY and late blight resistance which we had identified, were found to contain two or more resistance genes to these pathogens. According to the results of molecular tests, the clones were found to carry several markers of late blight and PVY resistance genes. Original interspecific potato hybrids with determined late blight resistance levels, cytoplasm type and availability of markers of genes for resistance to three pests can be used in further hybridization.
{"title":"Markers of genes for resistance to late blight, potato virus Y and potato cyst nematode identified in advanced interspecific potato hybrids","authors":"N. M. Zoteeva, O. Antonova, N. Klimenko, T. Gavrilenko","doi":"10.30901/2658-6266-2022-1-o1","DOIUrl":"https://doi.org/10.30901/2658-6266-2022-1-o1","url":null,"abstract":"Background. The harmful organisms affecting potato cause great economic damage in all areas where the crop is grown. One of the most economically important pathogens is the potato late blight (agent Phytophthora infestans Mont. (de Bary)). Also, Potato Virus Y (PVY) and potato cyst nematode (PCN) of the Ro1 pathotype, the only one that occurs in the Russian Federation, cause significant yield losses. Materials and methods. Phytopathological and molecular screening was carried out on original interspecific potato hybrids obtained in 11 crossing combinations, seven of which were studied for the first time. Hybrids resulted from crosses that involved Mexican and South American potato species as sources of late blight and PVY resistance. Field observations of late blight resistance were conducted during three seasons under high infection pressure. A part of plant material was screened in laboratory tests. PCR tests employed 9 DNA markers of genes associated with resistance to Ph. infestans, PVY and PCN of Ro1 pathotype used in our previous study. Results. In field evaluation, hybrid clones mostly expressed high, and a part of them moderate late blight resistance. Of the 6 markers of the used Rpi genes, at least two were detected in most clones, while some had 3 or 4 markers. Some clones were found to have markers of PCN resistance genes and of several genes for resistance to PVY. The cytoplasm type was determined for all plant material. Conclutions. Within a small but pedigree-diverse set of interspeсific hybrids, the markers of resistance genes to all three pests were found. A part of clones obtained by using the sources of PVY and late blight resistance which we had identified, were found to contain two or more resistance genes to these pathogens. According to the results of molecular tests, the clones were found to carry several markers of late blight and PVY resistance genes. Original interspecific potato hybrids with determined late blight resistance levels, cytoplasm type and availability of markers of genes for resistance to three pests can be used in further hybridization.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84057974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.9787/pbb.2022.10.1.31
N. Ardiarini, Damanhuri, A. Adiredjo
{"title":"Genetic Variability and Gene Action for Several Traits in F4 and F5 Population of Rice","authors":"N. Ardiarini, Damanhuri, A. Adiredjo","doi":"10.9787/pbb.2022.10.1.31","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.1.31","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47608860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.9787/pbb.2022.10.1.49
J. Kwak, J. Song, H. Seo
{"title":"Loss of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A Affects the Expression of Genes Involved in Fertilization","authors":"J. Kwak, J. Song, H. Seo","doi":"10.9787/pbb.2022.10.1.49","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.1.49","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47885340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Advances from Conventional to Modern Plant Breeding Methodologies","authors":"Sashi Lamichhane, Sapana Thapa","doi":"10.9787/pbb.2022.10.1.1","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.1.1","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46907976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.9787/pbb.2022.10.1.15
Rizwana Begum Syed Nabi, M. Lee, Sungup Kim, Jung-In Kim, Min Young Kim, Kwangmi Cho, Eunyoung Oh
{"title":"Physiological and Biochemical Responses of Diverse Peanut Genotypes under Drought Stress and Recovery at the Seedling Stage","authors":"Rizwana Begum Syed Nabi, M. Lee, Sungup Kim, Jung-In Kim, Min Young Kim, Kwangmi Cho, Eunyoung Oh","doi":"10.9787/pbb.2022.10.1.15","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.1.15","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44006711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.9787/pbb.2022.10.1.75
Yu-Hyeon Park, Tae-Hwan Jun
{"title":"Classification of Soybean [Glycine max (L.) Merr.] Seed Based on Deep Learning Using the YOLOv5 Model","authors":"Yu-Hyeon Park, Tae-Hwan Jun","doi":"10.9787/pbb.2022.10.1.75","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.1.75","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42673916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.9787/pbb.2022.10.1.62
Philip Bissiwu, Krishna Kulkarni, Kalpalatha Melmaiee, S. Elavarthi
{"title":"Physiological and Molecular Responses of Red Maple (Acer rubrum L.) Cultivars to Drought Stress","authors":"Philip Bissiwu, Krishna Kulkarni, Kalpalatha Melmaiee, S. Elavarthi","doi":"10.9787/pbb.2022.10.1.62","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.1.62","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48448430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.9787/pbb.2022.10.1.37
S. Rajendran, J. Bae, M. Park, J. Oh, H. W. Jeong, Young Koung Lee, S. Park
{"title":"Tomato Yield Effects of Reciprocal Hybridization of Solanum lycopersicum Cultivars M82 and Micro-Tom","authors":"S. Rajendran, J. Bae, M. Park, J. Oh, H. W. Jeong, Young Koung Lee, S. Park","doi":"10.9787/pbb.2022.10.1.37","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.1.37","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49653455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-16DOI: 10.30901/2658-6266-2021-4-o4
R. Rakhmangulov, N. Tikhonova
The breeding of ornamental plants as a branch of crop production is an integral part of the set of measures aimed at obtaining a wide range of different plants with high decorative characteristics. The main objective of this branch is the creation of plants that are attractive to the consumer and commercially characterized by such valuable biological features as adaptability, resistance to diseases, pests, frost and others. Most ornamental plant varieties were bred by means of traditional breeding methods such as selection, distant hybridization, clone breeding, radiation and chemically induced mutagenesis. However, the use of traditional breeding tools is limited by the potential for intraspecific variability. The development of modern biotechnological and genetic approaches to the breeding of new varieties has made it possible to modify the plant genotype at a qualitatively new level. The present review covers the directions in and methodology of modern ornamental plant breeding in Russia, ways of mobilizing the genetic resources of the main ornamental crops such as rose, clematis, canna, chrysanthemum, pelargonia, iris, daylily, tulip, lilac, and rhododendron. Aslo, the review offers examples of ornamental plant breeding work uderway in the leading specialized scientific institutions such as the Russian State Agrarian University – the K.A. Timiryazev Moscow Agricultural Academy, I.V. Michurin Federal Research Centre, Nikitsky Botanical Gardens – National Scientific Center of the RAS, Subtropical Scientific Center of the Russian Academy of Sciences, Botanical Garden of the M.V. Lomonosov Moscow State University, All-Russian Scientific Research Institute of Tobacco, Mahorka and Tobacco Products. The world and Russian flower and ornamental plants markets, the problem and methods of resolving the issue of quality import substitution, and prospects for the development of floriculture in Russia in the foreseeable future are also briefly considered.
{"title":"Breeding of ornamental plants in Russia","authors":"R. Rakhmangulov, N. Tikhonova","doi":"10.30901/2658-6266-2021-4-o4","DOIUrl":"https://doi.org/10.30901/2658-6266-2021-4-o4","url":null,"abstract":"The breeding of ornamental plants as a branch of crop production is an integral part of the set of measures aimed at obtaining a wide range of different plants with high decorative characteristics. The main objective of this branch is the creation of plants that are attractive to the consumer and commercially characterized by such valuable biological features as adaptability, resistance to diseases, pests, frost and others. Most ornamental plant varieties were bred by means of traditional breeding methods such as selection, distant hybridization, clone breeding, radiation and chemically induced mutagenesis. However, the use of traditional breeding tools is limited by the potential for intraspecific variability. The development of modern biotechnological and genetic approaches to the breeding of new varieties has made it possible to modify the plant genotype at a qualitatively new level. The present review covers the directions in and methodology of modern ornamental plant breeding in Russia, ways of mobilizing the genetic resources of the main ornamental crops such as rose, clematis, canna, chrysanthemum, pelargonia, iris, daylily, tulip, lilac, and rhododendron. Aslo, the review offers examples of ornamental plant breeding work uderway in the leading specialized scientific institutions such as the Russian State Agrarian University – the K.A. Timiryazev Moscow Agricultural Academy, I.V. Michurin Federal Research Centre, Nikitsky Botanical Gardens – National Scientific Center of the RAS, Subtropical Scientific Center of the Russian Academy of Sciences, Botanical Garden of the M.V. Lomonosov Moscow State University, All-Russian Scientific Research Institute of Tobacco, Mahorka and Tobacco Products. The world and Russian flower and ornamental plants markets, the problem and methods of resolving the issue of quality import substitution, and prospects for the development of floriculture in Russia in the foreseeable future are also briefly considered.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79543719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-15DOI: 10.30901/2658-6266-2021-4-o3
I. E. Khrabrov, O. Antonova, M. Shapovalov, L. G. Semenova
Background. Anthracnose black rot caused by the phytopathogenic fungus Colletotrichum acutatum Simmonds became an extremely harmful disease of strawberries in Southern Russia. The disease is widespread throughout the world, and relatively recently it appeared in Russia. Yield losses due to the disease reach up to 80%; besides, the pathogen causes significant plant losses in mother plantations. The most reliable protection against the pathogen is the cultivation of resistant varieties. In strawberries, resistance is controlled by different genes, including Rca2. To identify this gene, molecular markers STS_Rca2_240 and STS-Rca2_417 have been developed. The purpose of this study was to use the markers for screening the VIR collection of strawberry varieties at the VIR Maikop Experiment Station (Maikop ES VIR). Material and methods. The present work studied 135 varieties of Fragaria × ananassa (Duchesne ex Weston) Duchesne ex Rozier, 83 domestic and 52 foreign ones. The domestic varieties included 17 created at the Maikop ES. Plants were evaluated for anthracnose field resistance in the Republic of Adygea from 2018 to 2021. Molecular screening was performed using STS_Rca2_240 and STS-Rca2_417, the molecular markers closely linked to the Rca2 gene. Microsatellite primers EMFv020 were used to control the PCR. The cultivar ‘Sudarushka’, in which the presence of STS_Rca2_240 marker was described in the literature, served as a positive control. Results and discussion. The marker Rca2_240 was detected in 22 cultivars from 135 studied. Among domestic varieties, the frequency of the marker was 18.1%, while among the foreign varieties it was slightly lower and amounted to 13.0%. Among the 17 varieties created at the Maykop SE, the marker was found in three: ‘Majkopskaya rannyaya’, ‘Peryt’, and ‘Shapsugskaya’. The association of the diagnostic fragment with resistance was 73.0%. The marker efficiency was not very high due to the significant number of resistant varieties which do not generate the diagnostic fragments. The resistance in such varieties can be provided by other genes, for example, FaRCa1. The STS-Rca2_417 marker was not effective during screening. Conclusion. Twenty-two varieties with the STS_Rca2_240 marker were identified in the VIR collection, maintained at the Maykop ES VIR, 16 of which were resistant to C. acutatum. These varieties represent a valuable breeding material. The STS-Rca2_240 marker can be used as an important diagnostic trait for the certification of varieties.
背景。炭疽黑腐病是由植物病原菌炭疽菌(Colletotrichum acutatum Simmonds)引起的一种严重危害草莓的病害。这种疾病在世界各地广泛传播，最近在俄罗斯出现。因此病造成的产量损失高达80%;此外，病原菌还会对母林造成严重的植物损失。对病原菌最可靠的保护是培育抗病品种。在草莓中，抗性是由不同的基因控制的，包括Rca2。为了鉴定该基因，我们开发了分子标记STS_Rca2_240和STS-Rca2_417。本研究的目的是利用这些标记对VIR在Maikop实验站(Maikop ES VIR)草莓品种的VIR收集进行筛选。材料和方法。本文研究了135个Fragaria × ananassa (Duchesne ex Weston) Duchesne ex Rozier, 83个国内品种和52个国外品种。国内品种包括17种在Maikop ES培育的品种。对2018 - 2021年Adygea共和国的植物进行了炭疽病抗性评估。利用与Rca2基因密切相关的分子标记STS_Rca2_240和STS-Rca2_417进行分子筛选。微卫星引物EMFv020用于PCR控制。以文献报道存在STS_Rca2_240标记的品种‘Sudarushka’作为阳性对照。结果和讨论。在135个品种中的22个品种中检测到Rca2_240标记。在国内品种中，该标记出现的频率为18.1%，而在国外品种中，该标记出现的频率略低，为13.0%。在Maykop SE培育的17个品种中，有3个品种发现了该标记:‘Majkopskaya rannyaya’、‘Peryt’和‘Shapsugskaya’。诊断片段与耐药相关性为73.0%。标记效率不是很高，因为大量的耐药品种不能产生诊断片段。这些品种的抗性可以由其他基因提供，例如FaRCa1。STS-Rca2_417标记在筛选过程中无效。结论。在Maykop ES VIR数据库中，共鉴定出22个具有STS_Rca2_240标记的品种，其中16个品种对尖突镰刀菌具有抗性。这些品种是有价值的育种材料。STS-Rca2_240标记可作为品种认证的重要诊断性状。
{"title":"Molecular screening of the VIR strawberry varieties collection for the presence of a marker for the anthracnose black rot resistance gene Rca2","authors":"I. E. Khrabrov, O. Antonova, M. Shapovalov, L. G. Semenova","doi":"10.30901/2658-6266-2021-4-o3","DOIUrl":"https://doi.org/10.30901/2658-6266-2021-4-o3","url":null,"abstract":"Background. Anthracnose black rot caused by the phytopathogenic fungus Colletotrichum acutatum Simmonds became an extremely harmful disease of strawberries in Southern Russia. The disease is widespread throughout the world, and relatively recently it appeared in Russia. Yield losses due to the disease reach up to 80%; besides, the pathogen causes significant plant losses in mother plantations. The most reliable protection against the pathogen is the cultivation of resistant varieties. In strawberries, resistance is controlled by different genes, including Rca2. To identify this gene, molecular markers STS_Rca2_240 and STS-Rca2_417 have been developed. The purpose of this study was to use the markers for screening the VIR collection of strawberry varieties at the VIR Maikop Experiment Station (Maikop ES VIR). Material and methods. The present work studied 135 varieties of Fragaria × ananassa (Duchesne ex Weston) Duchesne ex Rozier, 83 domestic and 52 foreign ones. The domestic varieties included 17 created at the Maikop ES. Plants were evaluated for anthracnose field resistance in the Republic of Adygea from 2018 to 2021. Molecular screening was performed using STS_Rca2_240 and STS-Rca2_417, the molecular markers closely linked to the Rca2 gene. Microsatellite primers EMFv020 were used to control the PCR. The cultivar ‘Sudarushka’, in which the presence of STS_Rca2_240 marker was described in the literature, served as a positive control. Results and discussion. The marker Rca2_240 was detected in 22 cultivars from 135 studied. Among domestic varieties, the frequency of the marker was 18.1%, while among the foreign varieties it was slightly lower and amounted to 13.0%. Among the 17 varieties created at the Maykop SE, the marker was found in three: ‘Majkopskaya rannyaya’, ‘Peryt’, and ‘Shapsugskaya’. The association of the diagnostic fragment with resistance was 73.0%. The marker efficiency was not very high due to the significant number of resistant varieties which do not generate the diagnostic fragments. The resistance in such varieties can be provided by other genes, for example, FaRCa1. The STS-Rca2_417 marker was not effective during screening. Conclusion. Twenty-two varieties with the STS_Rca2_240 marker were identified in the VIR collection, maintained at the Maykop ES VIR, 16 of which were resistant to C. acutatum. These varieties represent a valuable breeding material. The STS-Rca2_240 marker can be used as an important diagnostic trait for the certification of varieties.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89511021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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