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Correlation and Path Analysis of Association among Yield, Micronutrients, and Protein Content in Rice Accessions Grown Under Aerobic Condition from Karnataka, India 印度卡纳塔克邦好氧条件下水稻产量、微量元素和蛋白质含量的相关及通径分析
Q3 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.9787/pbb.2023.11.2.117
N. Thuy, N. Trai, Bui Dang Khoa, Nguyen Hoang Xuan Thao, Vuong Tuan Phong, Q. Thi
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引用次数: 1
Anther Culture in Crop Plants: Progress and Perspectives 作物花药培养的进展与展望
Q3 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.9787/pbb.2023.11.2.69
M. T. Islam, M. Arif, Md. Toufiq Hasan, A. Robin
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引用次数: 0
Development of Cleaved Amplified Polymorphic Sequence Markers for Classifying Ginger (Zingiber officinale) Cultivars Using Reference Sequencing 利用参考序列对生姜品种进行分类的裂解扩增多态性序列标记的开发
Q3 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.9787/pbb.2023.11.2.130
Ji-Nam Kang, Gyeong-Hui Lee, Jin Yu, Mi-Hwa Choi, Simyung Lee
Ginger ( Zingiber officinale ) is grown worldwide in subtropical and tropical regions and primarily used as a spice and medicinal plant. Despite the economic importance of ginger, research on its molecular aspects is limited. Moreover, although ginger is mainly cultivated through vegetative propagation owing to poor flowering and infertility, few molecular markers have been identified to distinguish cultivars. In this research, we developed five Cleaved Amplified Polymorphic Sequence (CAPS) markers that can distinguish between the “Bongdong” ginger (Bg) cultivar, indigenous to South Korea
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引用次数: 1
The Morpho-Somatic and Chromosomal Changes in Colchicine Polyploidy Induction Colocasia esculenta var. Antiquorium 秋水仙碱多倍体诱导秋水仙草的形态、体细胞和染色体变化
Q3 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.9787/pbb.2023.11.2.105
F. Haring, M. Farid, S. Sudirman, M. Anshori
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引用次数: 0
Rice Breeding in Iran, Current Status and Future Perspective 伊朗水稻育种现状与展望
Q3 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.9787/pbb.2023.11.2.97
M. Modarresi
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引用次数: 0
Development of SNP Markers to Distinguish Various Watermelon Traits and Validation Using Fluidigm Genotyping Assay 西瓜不同性状的SNP标记的开发及fluidm基因分型试验验证
Q3 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.9787/pbb.2023.11.2.141
Sang-Min Yeo, Jeong-Eui Hong, M. A. Rahim, S. Shahriar, P. Choe, Sun-Kyun Jung, I. Nou
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引用次数: 0
The new directions in genetics, breeding and biotechnology of ornamental and berry crops in the N.I. Vavilov Institute of Plant Genetic Resources (VIR) Vavilov植物遗传资源研究所观赏和浆果作物遗传、育种和生物技术研究的新方向
Q3 Agricultural and Biological Sciences Pub Date : 2023-04-05 DOI: 10.30901/2658-6266-2022-4-o3
R. Rakhmangulov, I. V. Barabanov, M. V. Erastenkova, A. Ivanov, T. Kovalenko, K. M. Mezhina, I. A. Petrosyan, A. Kharchenko, D. Y. Shaimardanov, E. K. Shaimardanova, I. Anisimova, N. Tikhonova, Y. Ukhatova, E. Khlestkina
The use of modern breeding methods, biotechnology, and molecular genetics makes it possible to identify promising accessions with specified economically important traits at early pre-breeding stages. The success of creating new varieties depends on the availability of unique collections of plant genetic resources, information about genomes, possibility of in vitro cultivation with high regenerative capacity, and practical skills and competencies in this area. One of the advanced methods for accelerating the breeding process is genome editing using the CRISPR/Cas system. This method allows the effective modification of genes in order to obtain varieties with desired traits. In 2022, a new youth laboratory of genetics, breeding, biotechnology of ornamental and berry crops was set up at VIR as part of the National Project "Science and Universities". It is noteworthy that this event coincided with the 135th anniversary of the birth of N.I. Vavilov. The work of the laboratory is aimed at obtaining lines with desired properties for the further breeding process; identifying target genes of economically important traits for obtaining new varieties, lines, and hybrids; as well as creating protocols for the accelerated reproduction of virus-free material of commercially demanded varieties oriented towards import substitution. This review discusses current trends in breeding of ornamental and berry crops: e.g., flower color change in snapdragon and peony; flower aroma improvement in rose; architectonics change in actinidia; and increase of resistance to stress factors in blackberries, strawberries, and grapes.
利用现代育种方法、生物技术和分子遗传学,可以在早期育种前阶段确定具有特定经济重要性状的有前途的材料。培育新品种的成功与否取决于能否获得独特的植物遗传资源、基因组信息、具有高再生能力的离体培养的可能性以及该领域的实践技能和能力。加速育种过程的先进方法之一是使用CRISPR/Cas系统进行基因组编辑。这种方法允许有效地修改基因,以获得具有所需性状的品种。2022年,作为国家“科学与大学”项目的一部分,在VIR建立了一个新的观赏和浆果作物遗传、育种、生物技术青年实验室。值得注意的是,这一事件恰逢瓦维洛夫诞辰135周年。实验室的工作旨在为进一步的育种过程获得具有所需特性的品系;鉴定重要经济性状的靶基因,以获得新品种、品系和杂交种;此外,还制定了加速生产以进口替代为主的商业需求品种的无病毒材料的规程。本文综述了观赏和浆果作物育种的最新趋势:例如,金鱼龙和牡丹的花色变化;玫瑰花香气的改良;猕猴桃属植物的结构变化;在黑莓、草莓和葡萄中增加对压力因素的抵抗力。
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引用次数: 0
Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies 生物技术方法在VIR育种和细胞技术实验室培育蔬菜作物的可能性
Q3 Agricultural and Biological Sciences Pub Date : 2023-04-05 DOI: 10.30901/2658-6266-2022-4-o2
A. B. Kurina, A. Artemyeva
Basic and applied scientific research in plant cell technologies contribute to the successful development of agricultural plant breeding, which allows the creation of new forms of plants 2-4 times faster than by traditional breeding methods. To obtain inbred lines of most vegetable crops, about 5-7 cycles of self-pollination are required. As a result, the creation of a new cultivar/hybrid takes more than 10-12 years on an average. To successfully create a variety or hybrid, it is necessary to select parental pairs in the form of inbred lines. The VIR collection of vegetables and cucurbit crops includes 52,889 accessions, representatives of 29 families, 145 genera, and 610 species. The use of biotechnological methods is an important direction for accelerating the breeding of vegetable crops. Due to the relevance of introducing cell technologies into the breeding programs of the VIR Department of Genetic Resources of Vegetable and Cucurbit Crops, a Laboratory of Breeding and Cell Technologies was set up in 2022. The goal of the research to be performed at the new laboratory is to accelerate the creation of source material, cultivars and hybrids by combining traditional breeding methods and cell technologies. The objects of the study include cultivated forms and wild relatives of cabbage Brassica oleracea L., turnip Brassica rapa L., lettuce Lactuca L., tomato Lycopersicon Mill and vegetable sweet corn Zea mays var. saccharata Sturt. In the present review, we consider the main results of breeding cabbage, tomato, and lettuce which have been obtained through applying cell technologies. Despite the progress obtained, there are still several problems in this area. The lack of standardized, efficient and reproducible protocols for in vitro methods often hinders their practical use. The tasks facing the laboratory in creating the initial breeding material and new cultivars and hybrids with the use of both conventional methods and cell technologies are relevant and correspond to the world level.
植物细胞技术的基础和应用科学研究有助于农业植物育种的成功发展,使植物新形态的创造比传统育种方法快2-4倍。为了获得大多数蔬菜作物的自交系,大约需要5-7个自花授粉周期。因此,创造一个新品种/杂交品种平均需要10-12年以上的时间。为了成功地创造一个品种或杂种,必须以自交系的形式选择亲本对。VIR收集的蔬菜和瓜类作物包括52,889份资料,代表29科,145属和610种。利用生物技术方法是加快蔬菜作物育种的一个重要方向。由于将细胞技术引入VIR蔬菜和瓜类作物遗传资源部育种计划的相关性,于2022年成立了育种与细胞技术实验室。在新实验室进行的研究的目标是通过结合传统育种方法和细胞技术来加速原料、栽培品种和杂交品种的创造。研究对象包括白菜Brassica oleracea L.、萝卜Brassica rapa L.、生菜Lactuca L.、番茄lycopersico Mill和蔬菜甜玉米Zea mays var. saccharata Sturt的栽培形式和野生近缘种。本文综述了应用细胞技术在白菜、番茄和生菜育种方面取得的主要成果。尽管取得了进展,但在这方面仍存在一些问题。缺乏标准化、高效和可重复的体外方法方案往往阻碍其实际应用。实验室在利用常规方法和细胞技术创造最初的育种材料和新品种和杂交种方面面临的任务是相关的,符合世界水平。
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引用次数: 0
In vitro regeneration of grape 葡萄的离体再生
Q3 Agricultural and Biological Sciences Pub Date : 2023-04-05 DOI: 10.30901/2658-6266-2022-4-o1
T. Kovalenko, N. Tikhonova, E. Khlestkina, Y. Ukhatova
Considering the global and Russian experience in grape accessions preservation, one of the most reliable ways is the creation of a duplicate in vitro collection. However, in connection with the creation of duplicate grape collections and development of genome editing techniques, there is a need for selecting the most optimal medium composition that will ensure the maximum rate of callus formation and regeneration during the introduction of grape plants into in vitro culture. This will make it possible to obtain the necessary amount of material for further editing and subsequent regeneration of plants with knockout of target genes to improve economically valuable traits. For grapes, this is primarily an increase in resistance to powdery mildew caused by Uncinula necator Burill.The effect of active substances of biological and synthetic origin on the morphogenesis has been studied for many crops, including regenerant plants of the genus Vitis L. However, the genus Vitis is very diverse and heterogeneous in its genetic, physiological and morphological structure, as a result of which the recommended media and components for cultivation under in vitro conditions may not suit every cultivar. Local Russian grape cultivars are better suited to local growing conditions, so efforts should be focused on the development of techniques related to the preservation of local varieties in collections in vitro.Knowledge of genes controlling certain traits, as well as the availability of grape accessions whose genome has been sequenced, contribute to successful in silico analysis for creating editing constructs.
考虑到全球和俄罗斯在葡萄品种保存方面的经验,最可靠的方法之一是创建一个重复的体外收集。然而,随着重复葡萄收集的创建和基因组编辑技术的发展,需要选择最优的培养基组成,以确保在将葡萄植株引入离体培养过程中愈伤组织形成和再生的最大速率。这将有可能获得必要数量的材料,用于进一步编辑和随后通过敲除目标基因来再生植物,以改善有经济价值的性状。对于葡萄来说,这主要是增加了对白粉病的抵抗力,白粉病是由Uncinula necator Burill引起的。生物和合成来源的活性物质对许多作物形态发生的影响已经被研究过,包括葡萄属的再生植株。然而,葡萄属在遗传、生理和形态结构上非常多样化和异质性,因此在体外条件下推荐的培养基和成分可能不适合每个品种。俄罗斯当地的葡萄品种更适合当地的生长条件,因此应重点发展与保存当地品种的试管保存有关的技术。对控制某些性状的基因的了解,以及基因组已测序的葡萄品种的可用性,有助于成功地在硅分析中创建编辑结构。
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引用次数: 0
On the results of the 5th Vavilov International Conference (November 21–25, 2022) 关于第五届瓦维洛夫国际会议(2022年11月21日至25日)的成果
Q3 Agricultural and Biological Sciences Pub Date : 2023-03-19 DOI: 10.30901/2658-6266-2022-4-o6
E. Khlestkina, I. Loskutov, G. Batalova, M. Vishnyakova, I. Chukhina, Y. Ukhatova, A. Zavarzin
Under the auspices of the 5th Vavilov International Conference held in St. Petersburg on 21–25 November 2022, eight events were held on the conservation, development, study and practical use of plant genetic resources (PGR) collections, as well as on the scientific heritage of Nikolay Ivanovich Vavilov and the development of the scientific schools founded by him and the activities of his associates and followers. In view of the modern context of new trends in the development of science, fundamental knowledge, modern methods and technologies, as well as of climatic and economic challenges, the development of the scientific heritage of N.I. Vavilov plays a big role for scientific and technological development. A total of 185 oral presentations was made at the 5th Vavilov International Conference. Meetings of the Conference attracted over 330 participants. This publication reflects the main objectives and content of the performed activities and contains key recommendations emanating from the Conference, including (1) recommendations for the conservation, study and use of PGR, including those on the multidisciplinary basis; (2) recommendations on activities to ensure coordination in the field of conservation, research, breeding and seed production; (3) recommendations on the normative and legislative regulation in the sphere of breeding, seed production, development, maintenance and use of genetic resource collections; (4) recommendations on training, guidance and education. Presentations made at the Conference show that over the past five years there has been a significant increase in the use of modern methodical approaches, i.e. molecular-genetic, genomic and omics in the field of PGR research. All this provides a solid basis for the development of new breeding methods (Next-Generation Breeding). At the same time, it is emphasized that new breeding technologies based on the acquired knowledge, can lead to further progress not in isolation from, but in conjunction with the amassed heritage of classical breeding. Among the discussed and proposed issues, the Conference Resolution identifies those requiring special and urgent attention: (1) the need to legislate for the preservation and expansion of crop diversity in the State Register of Varieties and Hybrids of Agricultural Plants Approved for Use; (2) the inadmissibility of reducing the list of these crops; (3) the inadmissibility of reducing of the number of state variety testing sites; (4) the inadmissibility of reducing of time limits of state variety testing from three to two years or one year; (5) the need to bring the concepts of “genetic passportˮ and “genetic passportizationˮ used in legal and regulatory acts to the canonical understanding of “genetic passportˮ as a document reflecting individual genetic features of an organism (cultivar/hybrid/breed/strain), which make it possible to distinguish its genotype from that of other organisms (cultivars/hybrids/breeds/ strains) of a particular sp
在2022年11月21日至25日在圣彼得堡举行的第五届瓦维洛夫国际会议的主持下,举办了八场活动,主题是植物遗传资源(PGR)收藏的保护、开发、研究和实际利用,以及尼古拉·伊万诺维奇·瓦维洛夫的科学遗产、他所创立的科学流派的发展及其同事和追随者的活动。鉴于科学、基础知识、现代方法和技术发展的新趋势以及气候和经济挑战的现代背景,瓦维洛夫科学遗产的发展对科学和技术发展起着重要作用。第五届瓦维洛夫国际会议共作了185次口头报告。会议的会议吸引了330多名与会者。该出版物反映了已开展活动的主要目标和内容,并包含大会提出的主要建议,包括:(1)关于保护、研究和利用植物遗传资源的建议,包括基于多学科的建议;(2)关于在保护、研究、育种和种子生产领域确保协调的活动的建议;(3)遗传资源的育种、种子生产、开发、维护和利用等方面的规范和立法建议;(四)关于培训、指导和教育的建议。在会议上所作的介绍表明,在过去五年中,在植物遗传资源研究领域使用现代方法,即分子遗传学、基因组学和组学的情况有了显著增加。这些都为开发新的育种方法(下一代育种)提供了坚实的基础。与此同时,需要强调的是,以获得的知识为基础的新育种技术可以带来进一步的进步,而不是孤立地,而是与积累的经典育种遗产相结合。在讨论和提议的问题中,会议决议确定了需要特别和紧急关注的问题:(1)需要立法保护和扩大国家批准使用的农业植物品种和杂交品种登记册中的作物多样性;(二)不予减收的;(三)不允许减少国家品种试验场数量;(四)国家品种试验期限由3年缩短为2年或者1年的不得受理;(5)需要将法律法规中使用的“遗传护照”(genetic passport)和“遗传护照化”(genetic passportization)的概念纳入对“遗传护照”(genetic passport)的规范理解,即“遗传护照”是一种反映生物体(栽培种/杂交种/品种/品系)的个体遗传特征的文件,使其能够将其基因型与特定物种的其他生物体(栽培种/杂交种/品种/品系)的基因型区分开来,需要仔细制定每种作物的基因认证方法;考虑到所有类型的遗传标记(分子遗传标记(DNA标记),蛋白质和形态标记)。会议还注意到,在气候变化日益加剧及其突然不可预测的波动的情况下,需要在原地和非原地可靠地保护宝贵的植物遗传资源,以避免部分植物农业生物多样性不可逆转地丧失。此外,近期应在作物和CWR多样性中心、未开发地区、极端环境条件和容易受到人为影响的地区开展广泛的考察调查。
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Plant breeding and biotechnology
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