Pub Date : 2023-05-05DOI: 10.29235/1029-8940-2023-68-2-136-146
А. Ю. Карпаева, Anastasiya Yu. Karpaeva
We studied the seasonal changes in the taxonomic composition and abundance of phytoplankton in the littoral and pelagial zones of Obsterno Lake. A number of important differences recorded for the littoral and pelagic zones. The abundance in the littoral zone is higher in April, August and December. In other months it is higher in the pelagic zone. In the same months, the species richness in the littoral zone is also higher. These differences were influenced by such factors as biogenic elements (nitrogen and phosphorus), as well as the temperature factor.
{"title":"Seasonal changes in the taxonomical structure and abundance of phytoplankton in the pelagial and littoral zones of Obsterno Lake","authors":"А. Ю. Карпаева, Anastasiya Yu. Karpaeva","doi":"10.29235/1029-8940-2023-68-2-136-146","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-2-136-146","url":null,"abstract":"We studied the seasonal changes in the taxonomic composition and abundance of phytoplankton in the littoral and pelagial zones of Obsterno Lake. A number of important differences recorded for the littoral and pelagic zones. The abundance in the littoral zone is higher in April, August and December. In other months it is higher in the pelagic zone. In the same months, the species richness in the littoral zone is also higher. These differences were influenced by such factors as biogenic elements (nitrogen and phosphorus), as well as the temperature factor. ","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82683379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.29235/1029-8940-2023-68-2-169-176
V. M. Baitchorov, M. Moroz, Yu. G. Hihiniak, I. Y. Giginyak, A. A. Kulikova, Ja. V. Korzun
The taxonomic structure of the macrozoobenthos of 11 springs of the Grodno region has been established. There were identified 53 lower definable taxon (LDT) belonging to 4 types of aquatic invertebrates: Platyhelminthes – 1 LDT, Mollusca – 10, Annelida – 5, Arthropoda – 37 LDT. Among the identified animals, 23 species were first noted for the springs of the Grodno region. The fauna of the studied aquatic invertebrates of the springs of the Grodno region represented by rare and protected species of animals not only in Belarus, but also in Europe. Springs are a special type of natural reservoirs, which are inhabited by a specific fauna – one of the important components of the overall biological diversity of water bodies in Belarus. The most species of the fauna of the studied springs is formed by species exhibiting cryophilic properties, some of these animals are obvious relics of the Ice Age. The ways of penetration of these species into the modern fauna of Belarus are also not yet fully understood.
{"title":"Macrozoobenthos of spring complexes on the territory of the Grodno region","authors":"V. M. Baitchorov, M. Moroz, Yu. G. Hihiniak, I. Y. Giginyak, A. A. Kulikova, Ja. V. Korzun","doi":"10.29235/1029-8940-2023-68-2-169-176","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-2-169-176","url":null,"abstract":"The taxonomic structure of the macrozoobenthos of 11 springs of the Grodno region has been established. There were identified 53 lower definable taxon (LDT) belonging to 4 types of aquatic invertebrates: Platyhelminthes – 1 LDT, Mollusca – 10, Annelida – 5, Arthropoda – 37 LDT. Among the identified animals, 23 species were first noted for the springs of the Grodno region. The fauna of the studied aquatic invertebrates of the springs of the Grodno region represented by rare and protected species of animals not only in Belarus, but also in Europe. Springs are a special type of natural reservoirs, which are inhabited by a specific fauna – one of the important components of the overall biological diversity of water bodies in Belarus. The most species of the fauna of the studied springs is formed by species exhibiting cryophilic properties, some of these animals are obvious relics of the Ice Age. The ways of penetration of these species into the modern fauna of Belarus are also not yet fully understood. ","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81755698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-04DOI: 10.29235/1029-8940-2023-68-2-104-113
O. Y. Parkhomchuk, E. Fomina, E. E. Grigorieva
An expression vector containing the gene encoding the most common isoform Bet v 1.0101 of the Bet v 1 protein, the major birch pollen allergen, was created for a subsequent expression in the prokaryotic system of Escherichia coli. Total RNA from birch pollen collected in Belarus was used as a matrix. The expression vector pJC40-Веt v 1 was obtained using molecular-genetic methods: cloning, ligation, transformation. The specificity of the cloned fragment was confirmed by sequencing. During the study, 14 rare Escherichia coli codons were identified in the coding part of the cloned gene. The triplets were evenly arranged in the nucleotide sequence; codon clustering was observed only in two cases. The total percentage of rare triplets (8.75 %) and the calculated value of the codon adaptation level (0.57) allow us to predict a sufficiently efficient expression of the studied gene. The data obtained will be used in the synthesis of the recombinant polypeptide Bet v 1.0101.
构建了桦树花粉主要过敏原betv1蛋白最常见同种异构体betv 1.0101的表达载体，用于在大肠杆菌原核系统中表达。从白俄罗斯桦树花粉中提取总RNA作为基质。通过克隆、结扎、转化等分子遗传学方法获得表达载体pJC40-Веt v1。克隆片段的特异性经测序证实。在研究过程中，在克隆基因的编码部分鉴定出14个罕见的大肠杆菌密码子。三胞胎在核苷酸序列上排列均匀;密码子聚类仅在两例中观察到。罕见三胞胎的总百分比(8.75%)和密码子适应水平的计算值(0.57)使我们能够预测所研究基因的充分有效表达。所得数据将用于重组多肽Bet v 1.0101的合成。
{"title":"Obtaining an expression vector containing the nucleotide sequence of the gene encoding Bet v 1 – the major allergen of birch pollen","authors":"O. Y. Parkhomchuk, E. Fomina, E. E. Grigorieva","doi":"10.29235/1029-8940-2023-68-2-104-113","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-2-104-113","url":null,"abstract":"An expression vector containing the gene encoding the most common isoform Bet v 1.0101 of the Bet v 1 protein, the major birch pollen allergen, was created for a subsequent expression in the prokaryotic system of Escherichia coli. Total RNA from birch pollen collected in Belarus was used as a matrix. The expression vector pJC40-Веt v 1 was obtained using molecular-genetic methods: cloning, ligation, transformation. The specificity of the cloned fragment was confirmed by sequencing. During the study, 14 rare Escherichia coli codons were identified in the coding part of the cloned gene. The triplets were evenly arranged in the nucleotide sequence; codon clustering was observed only in two cases. The total percentage of rare triplets (8.75 %) and the calculated value of the codon adaptation level (0.57) allow us to predict a sufficiently efficient expression of the studied gene. The data obtained will be used in the synthesis of the recombinant polypeptide Bet v 1.0101.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87863761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-04DOI: 10.29235/1029-8940-2023-68-2-95-103
N. V. Baliuk, J. Kalatskaja, N. Laman
The effect of 24-epibrassinolide on salicylic acid and methyl jasmonate on the health of microclonally propagated potato plants to the Y virus, their physiological state, and the functions of the pro-/antioxidant system was studied. A synergistic interaction of immunostimulants with methyl jasmonate or salicylic acid with methyl jasmonate and salicylic acid in mixtures against viral infection was revealed, which includes a decrease in the incidence of potato Y virus, activation of plant growth and a low level of detection of pro- and antioxidant reactions that cause the development of oxidative epidemic stress. The revealed positive effects between brassinosteroids and methyl jasmonate in the suppression of viral infection and activation of growth processes are manifested by their interchangeable contribution to the regulation of the balance between plant growth and immunity.
{"title":"Protective reactions of potato plants infected with Y virus when treated with 24-epibrassinolide with salicylic acid and methyl jasmonate","authors":"N. V. Baliuk, J. Kalatskaja, N. Laman","doi":"10.29235/1029-8940-2023-68-2-95-103","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-2-95-103","url":null,"abstract":"The effect of 24-epibrassinolide on salicylic acid and methyl jasmonate on the health of microclonally propagated potato plants to the Y virus, their physiological state, and the functions of the pro-/antioxidant system was studied. A synergistic interaction of immunostimulants with methyl jasmonate or salicylic acid with methyl jasmonate and salicylic acid in mixtures against viral infection was revealed, which includes a decrease in the incidence of potato Y virus, activation of plant growth and a low level of detection of pro- and antioxidant reactions that cause the development of oxidative epidemic stress. The revealed positive effects between brassinosteroids and methyl jasmonate in the suppression of viral infection and activation of growth processes are manifested by their interchangeable contribution to the regulation of the balance between plant growth and immunity.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90900445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-11DOI: 10.29235/1029-8940-2023-68-1-75-88
I. Volotovski, S. V. Sukhaveyeva, E. Kabachevskaya
Three fundamental processes running in plant organisms under influence of environment (light, gravity) and key importance were considered. In the light case these are phytоchrome regulation and phototropism considering process is gravitropism. Phytochrome is responsible for regulatory reaction at the inhibition of which the plants cannot be normally developed. The plants do not need phototropism and gravitropism. They were elaborated by evolution as protective reactions to optimize the plant vitality. All these processes are realized according to one and the same logical scheme: stimulus reception, signaling processes in plant cell (transduction) and proper biological effect. According to this scheme the three reactions were considered. As the result the data adout the reception stages are principally different because receptor nature. Signaling processes proceed with participation of many low molecular and high molecular mediators to participate and biophysical, biochemical and genetic reactions. One fact attracts attention that the same mediators are involved to signaling ending by principally different final biological effect.This allows to suggest the existance in plant cell no separate chains for each stimulus but regulatory network formed by lateral and horizontal transduction chains.
{"title":"Biophysical mechanisms of intracellular signaling (transduction) in higher plants","authors":"I. Volotovski, S. V. Sukhaveyeva, E. Kabachevskaya","doi":"10.29235/1029-8940-2023-68-1-75-88","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-1-75-88","url":null,"abstract":"Three fundamental processes running in plant organisms under influence of environment (light, gravity) and key importance were considered. In the light case these are phytоchrome regulation and phototropism considering process is gravitropism. Phytochrome is responsible for regulatory reaction at the inhibition of which the plants cannot be normally developed. The plants do not need phototropism and gravitropism. They were elaborated by evolution as protective reactions to optimize the plant vitality. All these processes are realized according to one and the same logical scheme: stimulus reception, signaling processes in plant cell (transduction) and proper biological effect. According to this scheme the three reactions were considered. As the result the data adout the reception stages are principally different because receptor nature. Signaling processes proceed with participation of many low molecular and high molecular mediators to participate and biophysical, biochemical and genetic reactions. One fact attracts attention that the same mediators are involved to signaling ending by principally different final biological effect.This allows to suggest the existance in plant cell no separate chains for each stimulus but regulatory network formed by lateral and horizontal transduction chains.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87110875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-11DOI: 10.29235/1029-8940-2023-68-1-64-74
E. I. Mashkov, H. Gajduchenko, Y. Borisov
The analysis of the recent phylogeographic studies of Microtus arvalis is carried out to establish the post-glacial history of colonization of the common vole in the Central region of Europe. The authors have presented the original data on the genetic variability of the mtCytb gene of the common vole (Microtus arvalis s. l.) from 18 localities studied on the territory of Belarus. The genetic characteristics of 53 individuals of the common vole belonging to one eastern line of mitochondrial DNA were studied. It is shown that the population of the species in Belarus is characterized by a high level of genetic diversity comparable to that of other haplogroups of the eastern mtDNA line. The level of variability of mitochondrial fragments of the mtCytb gene of the common vole (Microtus arvalis) from Belarus turned outtobeq uite high (Hd ± SDHd = 0.97 ± 0.02), which is manifested in a large number of the identified mtDNA haplotypes (n = 41). The distribution of the identified haplotypes of Microtus arvalis across various landscape-geographical areas correlates well with the location of the most remote haplotypes on the parsimony network. The assumption is given about the re-settlement (about 9000 thousand years ago) and further settlement of Microtus arvalis on the modern territory of Belarus in two directions: from southeast to west and to northwest, as well as from north to southeast.
{"title":"Haplotypic diversity of the mtCytb gene of the common vole (Microtus arvalis sensu lato) in Belarus","authors":"E. I. Mashkov, H. Gajduchenko, Y. Borisov","doi":"10.29235/1029-8940-2023-68-1-64-74","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-1-64-74","url":null,"abstract":"The analysis of the recent phylogeographic studies of Microtus arvalis is carried out to establish the post-glacial history of colonization of the common vole in the Central region of Europe. The authors have presented the original data on the genetic variability of the mtCytb gene of the common vole (Microtus arvalis s. l.) from 18 localities studied on the territory of Belarus. The genetic characteristics of 53 individuals of the common vole belonging to one eastern line of mitochondrial DNA were studied. It is shown that the population of the species in Belarus is characterized by a high level of genetic diversity comparable to that of other haplogroups of the eastern mtDNA line. The level of variability of mitochondrial fragments of the mtCytb gene of the common vole (Microtus arvalis) from Belarus turned outtobeq uite high (Hd ± SDHd = 0.97 ± 0.02), which is manifested in a large number of the identified mtDNA haplotypes (n = 41). The distribution of the identified haplotypes of Microtus arvalis across various landscape-geographical areas correlates well with the location of the most remote haplotypes on the parsimony network. The assumption is given about the re-settlement (about 9000 thousand years ago) and further settlement of Microtus arvalis on the modern territory of Belarus in two directions: from southeast to west and to northwest, as well as from north to southeast.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91302029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.29235/1029-8940-2023-68-1-47-54
Y. Vinogradova, A. Spirydovich, V. Reshetnikov
The study of invasive alien species is carried out on a large scale because the economic and ecological damage caused by biological invasions is enormous. However, no unified approach to describing invasive populations has been developed so far. In the framework of alien plant inventory in Russia, the authors have developed the “Invasive Species Assessment Protocol”, which is a tool for assessing, categorizing and including in the “black”-lists any invasive alien plant species according to its impact on native species and natural biodiversity over a large geographic area. “The Protocol” contains five basic elements of the inventory: 1) taxonomic affiliation, life form, and natural range of species; 2) ecological impact; 3) current distribution and population size; 4) trends in abundance of populations or its size; and 5) ability to control alien species dissemination. “Protocol” is presented as the special blank for description of local invasive population. The description is very brief but, if necessary, additional information about the process of invasion of each alien species can be added to it. Specific additions to the protocol for some national economic objects: railroads, introduction institutions, Specially Protected Natural Areas (SPNA) are offered. Examples of using the Protocol during the inventory of invasive species on the Trans-Siberian Railway, in the NV Tsitsin Main Botanical Garden, and in the some Protected Natural Areas are given.
{"title":"Features of development of invasive species assessment and control protocols for different groups of plant communities","authors":"Y. Vinogradova, A. Spirydovich, V. Reshetnikov","doi":"10.29235/1029-8940-2023-68-1-47-54","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-1-47-54","url":null,"abstract":"The study of invasive alien species is carried out on a large scale because the economic and ecological damage caused by biological invasions is enormous. However, no unified approach to describing invasive populations has been developed so far. In the framework of alien plant inventory in Russia, the authors have developed the “Invasive Species Assessment Protocol”, which is a tool for assessing, categorizing and including in the “black”-lists any invasive alien plant species according to its impact on native species and natural biodiversity over a large geographic area. “The Protocol” contains five basic elements of the inventory: 1) taxonomic affiliation, life form, and natural range of species; 2) ecological impact; 3) current distribution and population size; 4) trends in abundance of populations or its size; and 5) ability to control alien species dissemination. “Protocol” is presented as the special blank for description of local invasive population. The description is very brief but, if necessary, additional information about the process of invasion of each alien species can be added to it. Specific additions to the protocol for some national economic objects: railroads, introduction institutions, Specially Protected Natural Areas (SPNA) are offered. Examples of using the Protocol during the inventory of invasive species on the Trans-Siberian Railway, in the NV Tsitsin Main Botanical Garden, and in the some Protected Natural Areas are given.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85222453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.29235/1029-8940-2023-68-1-7-14
V. V. Usenya, V. Shatravko, N. Blinova, G. M. Pomaz
Dynamics of pine stands dieback in 2016‒2021 in the forest fund of Belarus is presented. The area of clear sanitary cuttings was 112.3 thousand hectares.The results of field tests on attractiveness assessment of Belarusian synthetic pheromones “IPSVABOL V”, “IPSVABOL SH”, “MONVABOL” using barrier traps for monitoring the abundance of sharp-dentated (Ips acuminatus G yll.) a nd s ixtoothed (Ips sexdentatus Boern.) bark beetles and longhorn beetles (Monochamus spp.) in pine stands are presented. During insect abundance monitoring in pine stands, it was found that the synthetic pheromone “IPSVABOL V”, “IPSVABOL SH” and “MONVABOL” are highly attractive to beetles of the sharp-dentated, six-toothed bark and longhorn beetles of the genus Monochamus when monitoring their abundance in pine stands. It was revealed that the first generation flying of the sharpdentated and six-toothed bark beetles are the most widespread and higher by 1.8‒4.5 and 3.8‒5.1 times of the second generation flying of these stem pests, respectively.For the first time, scales for assessing the abundance and methods for pheromone monitoring of the sharp-dentated and six-toothed bark beetles, and longhorn beetles (Monochamus spp.) have been developed. The use of the developed scales in forest protection practice makes it possible to quickly control the abundance of these stem pests and their distribution in pine stands for appointment of forest protection measures.
{"title":"Attractiveness of synthetic pheromones for stem pests monitoring in pine stands in Belarus","authors":"V. V. Usenya, V. Shatravko, N. Blinova, G. M. Pomaz","doi":"10.29235/1029-8940-2023-68-1-7-14","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-1-7-14","url":null,"abstract":"Dynamics of pine stands dieback in 2016‒2021 in the forest fund of Belarus is presented. The area of clear sanitary cuttings was 112.3 thousand hectares.The results of field tests on attractiveness assessment of Belarusian synthetic pheromones “IPSVABOL V”, “IPSVABOL SH”, “MONVABOL” using barrier traps for monitoring the abundance of sharp-dentated (Ips acuminatus G yll.) a nd s ixtoothed (Ips sexdentatus Boern.) bark beetles and longhorn beetles (Monochamus spp.) in pine stands are presented. During insect abundance monitoring in pine stands, it was found that the synthetic pheromone “IPSVABOL V”, “IPSVABOL SH” and “MONVABOL” are highly attractive to beetles of the sharp-dentated, six-toothed bark and longhorn beetles of the genus Monochamus when monitoring their abundance in pine stands. It was revealed that the first generation flying of the sharpdentated and six-toothed bark beetles are the most widespread and higher by 1.8‒4.5 and 3.8‒5.1 times of the second generation flying of these stem pests, respectively.For the first time, scales for assessing the abundance and methods for pheromone monitoring of the sharp-dentated and six-toothed bark beetles, and longhorn beetles (Monochamus spp.) have been developed. The use of the developed scales in forest protection practice makes it possible to quickly control the abundance of these stem pests and their distribution in pine stands for appointment of forest protection measures.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87076488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.29235/1029-8940-2023-68-1-15-26
N. A. Yalouskaya, J. Kalatskaja, N. Laman, K. Hileuskaya, A. Kraskouski, V. Kulikouskaya
The article contains the results of study of the influence of added to culture medium silver-containing chitosan- based nanocomposites (Chitosan-Ag) at a dilution of 1:500 and 1:1000 (the mass ratio of the components is 50:1 for Chit- Ag 50:1 and 100:1 for Chit-Ag 100:1) on the development of potato microshoots and microclones with a formed root system. Potato microshoots cultivated for 4 weeks on nutrient medium modified with nanocomposites were characterized by slow development and the absence of rhizogenesis, which indicates the toxic effect of the studied nanocomposite concentrations. When replacing the standard nutrient medium with nanocomposites modified for a potato microclone with developed roots, the Chit-Ag 50:1 reduced the rate of growth and development of microclones compared to control and pure chitosan. The Chit- Ag 100:1 nanocomposite had no influence on the microclone growth compared to the control, but reduced the root biomass compared to chitosan. The preservation of photosynthetic pigments and proline concentrations with decreasing the hydrogen peroxide level indicates the absence of the toxic effect of silver-containing chitosan-based nanocomposites on the formed potato microclones. The chitosan concentration increase in the nanocomposite composition helps us to reduce the toxic effect due to the formation of a dense stabilizing shell that delays the silver ion generation.
本文研究了在含银壳聚糖基纳米复合材料(壳聚糖-Ag)培养基中以1:500和1:100的稀释比例(壳聚糖-Ag质量比为50:1，壳聚糖-Ag质量比为100:1)对马铃薯微芽和微无性系发育的影响，并对其根系形成进行了研究。土豆microshoots培养4周在营养培养基改性纳米复合材料的特点是发展缓慢和生根的缺席,这表明研究纳米复合材料的毒性作用浓度。与对照和纯壳聚糖相比，Chit-Ag 50:1的复合材料可以降低马铃薯微克隆的生长和发育速度。与壳聚糖相比，Chit- Ag 100:1纳米复合材料对微无性系生长没有影响，但减少了根生物量。随着过氧化氢浓度的降低，光合色素和脯氨酸浓度保持不变，表明含银壳聚糖纳米复合材料对形成的马铃薯微无性系没有毒性作用。纳米复合材料中壳聚糖浓度的增加有助于我们减少毒性效应，因为它形成了致密的稳定壳，延迟了银离子的生成。
{"title":"Influence of chitosan-stabilized silver nanoparticles on the physiological and biochemical state of potato plants (Solanum tuberosum L.) in vitro culture","authors":"N. A. Yalouskaya, J. Kalatskaja, N. Laman, K. Hileuskaya, A. Kraskouski, V. Kulikouskaya","doi":"10.29235/1029-8940-2023-68-1-15-26","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-1-15-26","url":null,"abstract":"The article contains the results of study of the influence of added to culture medium silver-containing chitosan- based nanocomposites (Chitosan-Ag) at a dilution of 1:500 and 1:1000 (the mass ratio of the components is 50:1 for Chit- Ag 50:1 and 100:1 for Chit-Ag 100:1) on the development of potato microshoots and microclones with a formed root system. Potato microshoots cultivated for 4 weeks on nutrient medium modified with nanocomposites were characterized by slow development and the absence of rhizogenesis, which indicates the toxic effect of the studied nanocomposite concentrations. When replacing the standard nutrient medium with nanocomposites modified for a potato microclone with developed roots, the Chit-Ag 50:1 reduced the rate of growth and development of microclones compared to control and pure chitosan. The Chit- Ag 100:1 nanocomposite had no influence on the microclone growth compared to the control, but reduced the root biomass compared to chitosan. The preservation of photosynthetic pigments and proline concentrations with decreasing the hydrogen peroxide level indicates the absence of the toxic effect of silver-containing chitosan-based nanocomposites on the formed potato microclones. The chitosan concentration increase in the nanocomposite composition helps us to reduce the toxic effect due to the formation of a dense stabilizing shell that delays the silver ion generation.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76645756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.29235/1029-8940-2023-68-1-27-37
T. Viks, I. Domanskaya, H. Martysiuk, L. F. Kаbashnikova
The objects of research were the first leaves of green seedlings of spring barley (Hordeum vulgare L.) of the varieties of Belarusian breeding − filmy (Magutny, Raider) and naked (Adamant). Seedlings of different age (3, 5, and 10 days old) were infected with spores of the fungus Bipolaris sorokiniana (Sacc.) Shoem. − a causative agent of helminthosporiasis, and were analyzed 2 days after infection. Varietal and ontogenetic differences between barley cultivars in morphological parameters of the first leaves, the content of photosynthetic pigments (chlorophylls (Chl) and carotenoids), and the ratio of spectral forms of Chl at the seedling stage were found. Different amplitudes of changes in the photosynthetic pigment content between the barley varieties with helminthosporiasis at different stages of development of the first leaf were established.
{"title":"Feature of the photosynthetic pigment fund in seedlings of different varieties of spring barley when affected by the fungus Bipolaris sorokiniana (Sacc.) Shoem","authors":"T. Viks, I. Domanskaya, H. Martysiuk, L. F. Kаbashnikova","doi":"10.29235/1029-8940-2023-68-1-27-37","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-1-27-37","url":null,"abstract":"The objects of research were the first leaves of green seedlings of spring barley (Hordeum vulgare L.) of the varieties of Belarusian breeding − filmy (Magutny, Raider) and naked (Adamant). Seedlings of different age (3, 5, and 10 days old) were infected with spores of the fungus Bipolaris sorokiniana (Sacc.) Shoem. − a causative agent of helminthosporiasis, and were analyzed 2 days after infection. Varietal and ontogenetic differences between barley cultivars in morphological parameters of the first leaves, the content of photosynthetic pigments (chlorophylls (Chl) and carotenoids), and the ratio of spectral forms of Chl at the seedling stage were found. Different amplitudes of changes in the photosynthetic pigment content between the barley varieties with helminthosporiasis at different stages of development of the first leaf were established.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75855367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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