Pub Date : 2022-11-01DOI: 10.29235/1029-8940-2022-67-4-359-373
S. Panteleev, L. Mozharovskaya, P. S. Kiryanov, D. Kagan, O. Baranov
The article presents the results of the mitochondrial DNA (30 837 n. r.) sequencing of the phytopathogenic fungi Phoma sp.1 – causative agent of Phoma blight of the pine and spruce plants cultivated in the forest nurseries. Annotation of the Phoma sp.1 mitochondrion showed 43 coding loci. Potential open reading frames (orf89, orf87, orf76 and orf108) and gene introns (cox3, nad1) are described. A comparative single genes analysis in the NCBI GenBank database showed, that Phoma sp.1 belongs to the Didymella spp., which can have Phoma anamorph. It has been shown that mitohondrial genes can be used as DNA markers for the diagnosis of Phoma and phoma-like fungi. Analysis of the mitochondrial synthenia of Phoma sp.1 and a related species (including phoma-like fungi), revealed significant structural rearrangements in mtDNA during phylogenesis.
{"title":"Structural and functional organisation of the phytopathogenic fungi Phoma sp.1 mitochondrial genome","authors":"S. Panteleev, L. Mozharovskaya, P. S. Kiryanov, D. Kagan, O. Baranov","doi":"10.29235/1029-8940-2022-67-4-359-373","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-4-359-373","url":null,"abstract":"The article presents the results of the mitochondrial DNA (30 837 n. r.) sequencing of the phytopathogenic fungi Phoma sp.1 – causative agent of Phoma blight of the pine and spruce plants cultivated in the forest nurseries. Annotation of the Phoma sp.1 mitochondrion showed 43 coding loci. Potential open reading frames (orf89, orf87, orf76 and orf108) and gene introns (cox3, nad1) are described. A comparative single genes analysis in the NCBI GenBank database showed, that Phoma sp.1 belongs to the Didymella spp., which can have Phoma anamorph. It has been shown that mitohondrial genes can be used as DNA markers for the diagnosis of Phoma and phoma-like fungi. Analysis of the mitochondrial synthenia of Phoma sp.1 and a related species (including phoma-like fungi), revealed significant structural rearrangements in mtDNA during phylogenesis.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75282325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-04DOI: 10.29235/1029-8940-2022-67-3-332-342
T. Artyukh
. The use of combinations of antibiotics is a promising area of antibiotic therapy of bacterial infections caused by antibiotic-resistant strains. The main goal of combined antibiotic therapy is to achieve a synergistic effect and to expand a spectrum of antibacterial activity against multi-resistant microorganisms. Due to the presence of various mechanisms of resistance in bacteria, even to drugs of the same group, the microbiological efficacy of antibiotic combinations is difficult to predict. Therefore, to select effective combinations of antibiotics, it is required to conduct a microbiological testing of isolates isolated from a particular patient. Currently, in the Republic of Belarus, the appointment of combined antibiotic therapy is carried out empirically, and in the event of its clinical ineffectiveness, the drugs are replaced. One of the reasons for a limited testing, with the availability of culture media and diagnostic materials, is the lack of regulatory and technical methods for identifying synergy adapted to local microbiological laboratories. The purpose of this publication was to analyze the literature data illustrating the relevance of analyzing the methods for studying the effects of synergy of antibacterial drugs and the possibility of their introduction into practical medicine. As a result of a search for publications in the PubMed database on the topic of increasing the antibacterial effect of antibiotics on the request the “checkerboard” method and the “time-kill” analysis for the period from 2016 to 2021, 947 results were found with an upward trend in research. “Checkerboard” and “time-kill” assays are the most common, reliable in vitro tests that reflect the effects of antibiotic combinations and the effects of antibiotic combinations with other compounds.
{"title":"Studying synergy of antibacterial drugs using the “checkerboard” method and the “time-kill” analysis","authors":"T. Artyukh","doi":"10.29235/1029-8940-2022-67-3-332-342","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-3-332-342","url":null,"abstract":". The use of combinations of antibiotics is a promising area of antibiotic therapy of bacterial infections caused by antibiotic-resistant strains. The main goal of combined antibiotic therapy is to achieve a synergistic effect and to expand a spectrum of antibacterial activity against multi-resistant microorganisms. Due to the presence of various mechanisms of resistance in bacteria, even to drugs of the same group, the microbiological efficacy of antibiotic combinations is difficult to predict. Therefore, to select effective combinations of antibiotics, it is required to conduct a microbiological testing of isolates isolated from a particular patient. Currently, in the Republic of Belarus, the appointment of combined antibiotic therapy is carried out empirically, and in the event of its clinical ineffectiveness, the drugs are replaced. One of the reasons for a limited testing, with the availability of culture media and diagnostic materials, is the lack of regulatory and technical methods for identifying synergy adapted to local microbiological laboratories. The purpose of this publication was to analyze the literature data illustrating the relevance of analyzing the methods for studying the effects of synergy of antibacterial drugs and the possibility of their introduction into practical medicine. As a result of a search for publications in the PubMed database on the topic of increasing the antibacterial effect of antibiotics on the request the “checkerboard” method and the “time-kill” analysis for the period from 2016 to 2021, 947 results were found with an upward trend in research. “Checkerboard” and “time-kill” assays are the most common, reliable in vitro tests that reflect the effects of antibiotic combinations and the effects of antibiotic combinations with other compounds.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85471506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-04DOI: 10.29235/1029-8940-2022-67-3-321-331
Y. Strakh, O. S. Ignatovets
The article describes the climatic conditions and the geographical zones of cloudberry (Rubus chamaemorus L.) growth. A trend towards cultural and semi-cultural cultivation of this species was noted. The current state of research on the chemical composition of various parts of cloudberry squat, growing in various environmental conditions, is highlighted. A systematic assessment of accumulation of biologically active substances (vitamins, fatty acids, flavonoids, anthocyanins, proanthocyanidins, phenolic acids) of this plant of northern and southern populations was carried out. The role of secondary metabolites in the formation of the biological potential of R. chamaemorus L. fruits and leaves is described. High antioxidant and antiradical activities of cloudberry fruit extracts in in vitro and in vivo model systems are also noted. The review presents the results of scientific works devoted to the study of the antimicrobial activity of fruits against Helicobacter pillory, Staphylococcus aureus, Staphylococcus epidermis, Bacillus subtilis, Campylobacter jejuni, Micrococcus luteus, Escherichia coli, Candida albicans. There is a lack of research of secondary metabolites of the plant on the southern border of the range. The biological significance of fruits and ground leaf mass, as well as the prospects for cultivation on the southern border of the range and their practical use in the pharmaceutical and food industries are highlighted.
{"title":"Chemical composition and biological activity of metabolites of Rubus chamaemorus L.","authors":"Y. Strakh, O. S. Ignatovets","doi":"10.29235/1029-8940-2022-67-3-321-331","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-3-321-331","url":null,"abstract":" The article describes the climatic conditions and the geographical zones of cloudberry (Rubus chamaemorus L.) growth. A trend towards cultural and semi-cultural cultivation of this species was noted. The current state of research on the chemical composition of various parts of cloudberry squat, growing in various environmental conditions, is highlighted. A systematic assessment of accumulation of biologically active substances (vitamins, fatty acids, flavonoids, anthocyanins, proanthocyanidins, phenolic acids) of this plant of northern and southern populations was carried out. The role of secondary metabolites in the formation of the biological potential of R. chamaemorus L. fruits and leaves is described. High antioxidant and antiradical activities of cloudberry fruit extracts in in vitro and in vivo model systems are also noted. The review presents the results of scientific works devoted to the study of the antimicrobial activity of fruits against Helicobacter pillory, Staphylococcus aureus, Staphylococcus epidermis, Bacillus subtilis, Campylobacter jejuni, Micrococcus luteus, Escherichia coli, Candida albicans. There is a lack of research of secondary metabolites of the plant on the southern border of the range. The biological significance of fruits and ground leaf mass, as well as the prospects for cultivation on the southern border of the range and their practical use in the pharmaceutical and food industries are highlighted.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73355060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-04DOI: 10.29235/1029-8940-2022-67-3-309-320
I. Volotovski, S. Pinchuk, I. Vasilevich
Тhe mechanisms of differentiation of mesenchimal stem cells into the somatic cells of organs and tissues underlying embryogenesis and natural reparation processes and providing the structural and functional homeostasis of cells are considered. The data on adipogenic, osteogenic, chondrogenic, miogenic, and endothelial differentiations are given, which results in the formation of the cells of mesodermal origin in organism. The problem is discussed, how the transcription factors control each type of differentiation and participatе in them using various regulatory biomolecules, transcription factors, cytokines, and chimokins being in complicate permanent interactions and forming the integrity regulatory network. The participation in differentiation processes of a number of transcription factors (Runx2, Sox9, PPARγ, MyoD, GATA4 и GATA6) is discussed, the expression of which is under a permanent chemical control within the cellular regulatory network.
{"title":"Transcription factors – key regulatory biomolecules determining the differentiation of mesenchimal stem cells into the somatic cells of organs and tissues","authors":"I. Volotovski, S. Pinchuk, I. Vasilevich","doi":"10.29235/1029-8940-2022-67-3-309-320","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-3-309-320","url":null,"abstract":"Тhe mechanisms of differentiation of mesenchimal stem cells into the somatic cells of organs and tissues underlying embryogenesis and natural reparation processes and providing the structural and functional homeostasis of cells are considered. The data on adipogenic, osteogenic, chondrogenic, miogenic, and endothelial differentiations are given, which results in the formation of the cells of mesodermal origin in organism. The problem is discussed, how the transcription factors control each type of differentiation and participatе in them using various regulatory biomolecules, transcription factors, cytokines, and chimokins being in complicate permanent interactions and forming the integrity regulatory network. The participation in differentiation processes of a number of transcription factors (Runx2, Sox9, PPARγ, MyoD, GATA4 и GATA6) is discussed, the expression of which is under a permanent chemical control within the cellular regulatory network.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74802355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-04DOI: 10.29235/1029-8940-2022-67-3-304-308
Y. V. Kandratau, U. I. Torchyk, A. A. Kuraksina
The influence of the age of the scion and the insulating material on the survival rate and morphometric para- meters of annual grafted plants is shown, using the example of Larix kaempferi ‘Blue Dwarf’. It has been established that when grafting decorative forms of the genus Larix Mill. the most effective is the use of annual shoots as a scion with isolation of the grafting site with an elastic polyethylene film, which ensures 100 % survival and optimal development of the crown.The use of 2-year-old shoots as a scion with a shortened by 2/3 annual growth does not lead to a significant change in sur- vival rate and a decrease in the height and diameter of annual grafted plants, but it allows to increase the density of the crown. The use of these shoots as a graft can be recommended for propagation of garden forms, the grafting of which is complicated due to thin annual shoots.
{"title":"Features of grafting decorative forms of the genus Larix Mill","authors":"Y. V. Kandratau, U. I. Torchyk, A. A. Kuraksina","doi":"10.29235/1029-8940-2022-67-3-304-308","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-3-304-308","url":null,"abstract":"The influence of the age of the scion and the insulating material on the survival rate and morphometric para- meters of annual grafted plants is shown, using the example of Larix kaempferi ‘Blue Dwarf’. It has been established that when grafting decorative forms of the genus Larix Mill. the most effective is the use of annual shoots as a scion with isolation of the grafting site with an elastic polyethylene film, which ensures 100 % survival and optimal development of the crown.The use of 2-year-old shoots as a scion with a shortened by 2/3 annual growth does not lead to a significant change in sur- vival rate and a decrease in the height and diameter of annual grafted plants, but it allows to increase the density of the crown. The use of these shoots as a graft can be recommended for propagation of garden forms, the grafting of which is complicated due to thin annual shoots.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73513891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-04DOI: 10.29235/1029-8940-2022-67-3-343-344
A. Editorial
.
．
{"title":"Nikolay Aleksandrovich Kartel (To the 85th Anniversary)","authors":"A. Editorial","doi":"10.29235/1029-8940-2022-67-3-343-344","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-3-343-344","url":null,"abstract":"<jats:p>.</jats:p>","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80190057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-02DOI: 10.29235/1029-8940-2022-67-3-285-292
N. Naumovich, Z. Aleschenkova, I. N. Ananyeva, H. V. Safronava
{"title":"Cultivation of halotolerant bacteria as a basis of the microbial fertilizer “Biotilia”","authors":"N. Naumovich, Z. Aleschenkova, I. N. Ananyeva, H. V. Safronava","doi":"10.29235/1029-8940-2022-67-3-285-292","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-3-285-292","url":null,"abstract":"","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83736274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-02DOI: 10.29235/1029-8940-2022-67-3-263-273
М. Герасимович, Е. И. Рыбинская, И. А. Овчинников, Е. Л. Недведь, Ж. Н. Калацкая, К. С. Гилевская, В. В. Николайчук, Н. А. Ламан, K. Herasimovich, K. I. Rybinskaya, Igor A. Ovchinnikov, H. Nedved, J. Kalatskaja, K. Hileuskaya, V. V. Nikalaichuk, N. Laman
The effect of seed treatment with various materials of chitosan and hydroxycinnamic acids on the growth of barley seedlings was evaluated. It was shown that chitosan-hydroxycinnamic acid conjugates and nanoparticles had no negative effect on the initial stages of the growth of barley seedlings. Adaptation of seedlings after 24 h exposure to 4 % NaCl in the samples of conjugates of 30 kDa chitosan with caffeic and ferulic acids was more effective. There was a decrease in proline contents and restoration of the growth activity under post-stress conditions.
{"title":"Influence of chitosan and hydroxycinnamic acids conjugates and nanoparticles on the growth of barley seedlings and proline contents under saline stress","authors":"М. Герасимович, Е. И. Рыбинская, И. А. Овчинников, Е. Л. Недведь, Ж. Н. Калацкая, К. С. Гилевская, В. В. Николайчук, Н. А. Ламан, K. Herasimovich, K. I. Rybinskaya, Igor A. Ovchinnikov, H. Nedved, J. Kalatskaja, K. Hileuskaya, V. V. Nikalaichuk, N. Laman","doi":"10.29235/1029-8940-2022-67-3-263-273","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-3-263-273","url":null,"abstract":"The effect of seed treatment with various materials of chitosan and hydroxycinnamic acids on the growth of barley seedlings was evaluated. It was shown that chitosan-hydroxycinnamic acid conjugates and nanoparticles had no negative effect on the initial stages of the growth of barley seedlings. Adaptation of seedlings after 24 h exposure to 4 % NaCl in the samples of conjugates of 30 kDa chitosan with caffeic and ferulic acids was more effective. There was a decrease in proline contents and restoration of the growth activity under post-stress conditions.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85632038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-02DOI: 10.29235/1029-8940-2022-67-3-293-303
Dz. G. Grummo, A. Pugachevsky, S. G. Rusetsky, N. A. Zeliankevich
{"title":"Analysis of forest cover dynamics in Belarus based on remote sensing data","authors":"Dz. G. Grummo, A. Pugachevsky, S. G. Rusetsky, N. A. Zeliankevich","doi":"10.29235/1029-8940-2022-67-3-293-303","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-3-293-303","url":null,"abstract":"","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74336486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-02DOI: 10.29235/1029-8940-2022-67-3-274-284
A. N. Morozova, A. Akhremchuk, N. Golovnyova
The molecular-genetic analysis of the bacterial genome of the strain B. longum BIM B-813D distinguished by a high level of β-galactosidase production was performed. Genes Bgal_small_N, lacZ1, bgaB1, bgaB2 and bgaB3, and lacZ2, encoding the synthesis of β-galactosidases, were revealed in the deciphered genome. It was shown that the genes lacZ1, bgaB2, and bgaB3 characterized by an enhanced degree of similarity to the genes of closely related bifidobacterial species, presumably code for the enzymes catalyzing the specific reactions of hydrolysis and transglycosylation of carbohydrates. It was found that the enzymes BgaB1, BgaB2 and BgaB3 belong to the GH42 family of glycosyl hydrolases, whereas the enzymes LacZ1 and LacZ2 – to the GH2 family. The genome domains responsible for the synthesis of β-galactosidases in the strain B. longum BIM B-813D were studied in detail. A comparative analysis of the locus of lacZ1 in B. longum BIM B-813D and the similar genome fragment AS143_01230 from B. longum subsp. longum MC-42 detected the presence of the transposase gene ISL3 in the former strain. It was suggested that the insertion of the sequence of ISL3 in the lacZ1 locus resulted in the modified gene expression and the increased production of β-galactosidase in the strain B. longum BIM B-813D.
{"title":"Мolecular-genetic analysis of determinants encoding β-galactosidases of bacteria Bifidobacterium longum BIM B-813 D","authors":"A. N. Morozova, A. Akhremchuk, N. Golovnyova","doi":"10.29235/1029-8940-2022-67-3-274-284","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-3-274-284","url":null,"abstract":"The molecular-genetic analysis of the bacterial genome of the strain B. longum BIM B-813D distinguished by a high level of β-galactosidase production was performed. Genes Bgal_small_N, lacZ1, bgaB1, bgaB2 and bgaB3, and lacZ2, encoding the synthesis of β-galactosidases, were revealed in the deciphered genome. It was shown that the genes lacZ1, bgaB2, and bgaB3 characterized by an enhanced degree of similarity to the genes of closely related bifidobacterial species, presumably code for the enzymes catalyzing the specific reactions of hydrolysis and transglycosylation of carbohydrates. It was found that the enzymes BgaB1, BgaB2 and BgaB3 belong to the GH42 family of glycosyl hydrolases, whereas the enzymes LacZ1 and LacZ2 – to the GH2 family. The genome domains responsible for the synthesis of β-galactosidases in the strain B. longum BIM B-813D were studied in detail. A comparative analysis of the locus of lacZ1 in B. longum BIM B-813D and the similar genome fragment AS143_01230 from B. longum subsp. longum MC-42 detected the presence of the transposase gene ISL3 in the former strain. It was suggested that the insertion of the sequence of ISL3 in the lacZ1 locus resulted in the modified gene expression and the increased production of β-galactosidase in the strain B. longum BIM B-813D.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89312679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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