Pub Date : 2024-02-08DOI: 10.29235/1029-8940-2024-69-1-25-35
V. М. Baitchorov, М. D. Moroz, Y. G. Hihiniak, I. Y. Giginyak, A. A. Kulikova, J. V. Korzun
New data on aquatic invertebrates of the spring ecosystems of the Minsk region are presented. 104 lowest defined taxa (LDT) species and forms of representatives of macrozoobenthos and pleistone complexes belonging to 4 phyla of aquatic invertebrates were found: Platyhelminthes – 2, Mollusca – 20, Annelida – 7, Arthropoda – 75 species and forms. 75 taxonomic elements have been identified before the species. The average number of identified aquatic invertebrate species in the studied springs of the Minsk region was 12‒13 species, and the number of 205 specimens. The sources are inhabited by protected species of animals listed in the Red Book of Belarus and the Red Lists of a number of Western European countries. It was concluded that the fauna of aquatic invertebrate animals is rich and represented by a number of species rare in Belarus and Europe.
{"title":"Fauna of aquatic invertebrates of the spring ecosystems in the Minsk region","authors":"V. М. Baitchorov, М. D. Moroz, Y. G. Hihiniak, I. Y. Giginyak, A. A. Kulikova, J. V. Korzun","doi":"10.29235/1029-8940-2024-69-1-25-35","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-25-35","url":null,"abstract":"New data on aquatic invertebrates of the spring ecosystems of the Minsk region are presented. 104 lowest defined taxa (LDT) species and forms of representatives of macrozoobenthos and pleistone complexes belonging to 4 phyla of aquatic invertebrates were found: Platyhelminthes – 2, Mollusca – 20, Annelida – 7, Arthropoda – 75 species and forms. 75 taxonomic elements have been identified before the species. The average number of identified aquatic invertebrate species in the studied springs of the Minsk region was 12‒13 species, and the number of 205 specimens. The sources are inhabited by protected species of animals listed in the Red Book of Belarus and the Red Lists of a number of Western European countries. It was concluded that the fauna of aquatic invertebrate animals is rich and represented by a number of species rare in Belarus and Europe.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":"40 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139853476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-08DOI: 10.29235/1029-8940-2024-69-1-36-46
Р. V. Kuzmitskaya, К. S. Karaleva, О. Y. Urbanovich
In plants, stress-associated proteins (SAP) are involved in response to adverse biotic and abiotic factors. In order to study the drought effect on changes in the SAP expression level in apple trees and their involvement in stress response, an expression level of fourteen genes belonging to this family was analyzed using qPCR. Their participation in a stress response to drought in the genome of the MM-106 apple tree rootstock was shown. At that, the genes demonstrated a tendency to increase their expression by the fourth hour of drought exposure followed by its decrease by the twenty-fourth hour.A comparison of the data obtained during the described experiment with the data obtained when modeling drought under milder conditions showed that under more severe drought conditions, an expression of a greater number of gene encoding stress-associated proteins increases, and a more pronounced response occurs. Evaluation of the effect of an identity degree of nucleotide sequences of gene encoding stress-associated proteins on the similarity of their expression profiles under drought exposure did not reveal any direct relationship between the primary structure of genes and the nature of their expression.In the structure of stress-associated proteins encoded by the genes most strongly response to drought in Malus, Solanum lycopersicum, Gossypium hirsutum, and Cucumis sativus, the A20-AN1 type domain occurs more frequently than other types of zinc finger domains. Analysis of the similarity of stress-associated protein sequences and the expression levels of genes encoding them under drought conditions in different plant species (apple, cotton, tomato, and cucumber) suggests that in each species, the evolution of adaptation mechanisms, including SAP encoding genes, occurred independently and, most likely, after separation of those species from their common ancestor. The results obtained will make it possible to clarify the role of individual genes, encoding SAP, in the formation of an apple stress response to the drought effect.
在植物中，胁迫相关蛋白（SAP）参与了对不利的生物和非生物因素的响应。为了研究干旱对苹果树中 SAP 表达水平变化的影响及其在应激反应中的参与情况，使用 qPCR 分析了属于该家族的 14 个基因的表达水平。结果表明，在 MM-106 苹果树砧木的基因组中，这些基因参与了对干旱的应激反应。将所述实验中获得的数据与在较温和条件下模拟干旱时获得的数据进行比较后发现，在更严重的干旱条件下，更多编码应激相关蛋白的基因表达量会增加，并出现更明显的反应。评估编码应激相关蛋白的基因核苷酸序列的同一性对干旱条件下基因表达谱相似性的影响，没有发现基因的一级结构与基因表达的性质有任何直接关系。在Malus、Solanum lycopersicum、Gossypium hirsutum和Cucumis sativus对干旱反应最强烈的基因所编码的应激相关蛋白结构中，A20-AN1型结构域出现的频率高于其他类型的锌指结构域。对不同植物物种（苹果、棉花、番茄和黄瓜）中干旱条件下胁迫相关蛋白序列和编码这些蛋白的基因表达水平的相似性分析表明，在每个物种中，适应机制（包括 SAP 编码基因）的进化都是独立发生的，而且很可能是在这些物种从其共同祖先分离出来之后发生的。研究结果将有助于明确编码 SAP 的单个基因在形成苹果对干旱影响的应激反应中的作用。
{"title":"Effect of moisture deficiency on the apple stress-associated protein expression level .","authors":"Р. V. Kuzmitskaya, К. S. Karaleva, О. Y. Urbanovich","doi":"10.29235/1029-8940-2024-69-1-36-46","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-36-46","url":null,"abstract":"In plants, stress-associated proteins (SAP) are involved in response to adverse biotic and abiotic factors. In order to study the drought effect on changes in the SAP expression level in apple trees and their involvement in stress response, an expression level of fourteen genes belonging to this family was analyzed using qPCR. Their participation in a stress response to drought in the genome of the MM-106 apple tree rootstock was shown. At that, the genes demonstrated a tendency to increase their expression by the fourth hour of drought exposure followed by its decrease by the twenty-fourth hour.A comparison of the data obtained during the described experiment with the data obtained when modeling drought under milder conditions showed that under more severe drought conditions, an expression of a greater number of gene encoding stress-associated proteins increases, and a more pronounced response occurs. Evaluation of the effect of an identity degree of nucleotide sequences of gene encoding stress-associated proteins on the similarity of their expression profiles under drought exposure did not reveal any direct relationship between the primary structure of genes and the nature of their expression.In the structure of stress-associated proteins encoded by the genes most strongly response to drought in Malus, Solanum lycopersicum, Gossypium hirsutum, and Cucumis sativus, the A20-AN1 type domain occurs more frequently than other types of zinc finger domains. Analysis of the similarity of stress-associated protein sequences and the expression levels of genes encoding them under drought conditions in different plant species (apple, cotton, tomato, and cucumber) suggests that in each species, the evolution of adaptation mechanisms, including SAP encoding genes, occurred independently and, most likely, after separation of those species from their common ancestor. The results obtained will make it possible to clarify the role of individual genes, encoding SAP, in the formation of an apple stress response to the drought effect.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139851316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-08DOI: 10.29235/1029-8940-2024-69-1-15-24
N. V. Baliuk, N. Laman, J. Kalatskaja
The effect of epibrassinolide in combination with salicylic acid and methyl jasmonate on the formation of complex resistance of clonally micropropagated potato plants of the Briz variety to the potato virus Y and to moisture lack, their physiological state, the functioning of the pro-/antioxidant system, as well as on the productivity and quality of the obtained mini-tubers was studied. It was revealed that antiviral resistance under combined stress conditions is formed only in the presence of epibrassinolide that triggers a series of protective mechanisms through accumulation of hydrogen peroxide, phenolic compounds and an increase in peroxidase activity, while maintaining productivity and improving the quality of the products obtained. The use of a three-component mixture is accompanied by the largest mass and a number of potato minitubers, while its protective effect against a viral infection is not manifested.
研究了表木栓内酯与水杨酸和茉莉酸甲酯结合使用对克隆微繁殖的布里兹马铃薯植株对马铃薯病毒 Y 和缺水形成的复合抗性、其生理状态、原/抗氧化剂系统的功能以及对所获得的微型管的产量和质量的影响。结果表明，在综合胁迫条件下，只有在表紫苏内酯存在的情况下才能形成抗病毒性，它通过积累过氧化氢、酚类化合物和提高过氧化物酶活性来触发一系列保护机制，同时保持生产率并提高所获得产品的质量。在使用三组份混合物的同时，马铃薯小立方体的质量和数量最大，而其对病毒感染的保护作用却没有体现出来。
{"title":"Physiological and biochemical features of implementation of the adaptive potential of clonally micropropagated potato plants using immunostimulants","authors":"N. V. Baliuk, N. Laman, J. Kalatskaja","doi":"10.29235/1029-8940-2024-69-1-15-24","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-15-24","url":null,"abstract":"The effect of epibrassinolide in combination with salicylic acid and methyl jasmonate on the formation of complex resistance of clonally micropropagated potato plants of the Briz variety to the potato virus Y and to moisture lack, their physiological state, the functioning of the pro-/antioxidant system, as well as on the productivity and quality of the obtained mini-tubers was studied. It was revealed that antiviral resistance under combined stress conditions is formed only in the presence of epibrassinolide that triggers a series of protective mechanisms through accumulation of hydrogen peroxide, phenolic compounds and an increase in peroxidase activity, while maintaining productivity and improving the quality of the products obtained. The use of a three-component mixture is accompanied by the largest mass and a number of potato minitubers, while its protective effect against a viral infection is not manifested.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":"18 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139851865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-08DOI: 10.29235/1029-8940-2024-69-1-57-67
A. A. Famina, A. N. Zainchkovskaya, Р. V. Kuzmitskaya, О. Y. Urbanovich, Р. А. Pashkevich, L. S. Sidor, В. Y. Anoshenko, L. V. Goncharova
Based on the sequence of the Golden Delicious apple genome, whose nucleotide sequence is located in the GenBank database, the design of molecular markers of SSR type that limit the area of tetra- and hexanucleotide repeats was carried out in silico. The most informative of them were selected, which showed clear peaks on capillary electrophoresis and made it possible to reveal a high level of polymorphism in certain apple genome regions. The developed SSR markers can be effectively used for the genetic diversity assession and DNA identification of apple varieties, establishment of the varietal correspondence. These markers were used to analyze the genetic diversity of apple trees in the old orchard of the Central Botanical Garden of the National Academy of Sciences of Belarus (CBG) where trees were planted from 1932 to 1940. It is shown that the trees growing in the garden are genetically close to old local varieties, as well as to individual foreign ones. It is established that among the trees of the CBG old orchard there are such varieties as Antonovka obyknovennaya, Minskoe, Papirovka. Wealthy, Melba, Bogatyr’. The obtained results will be used to save the genetic resources of the fruit crop valuable for the country, which is an apple tree, as well as to develop a design project of a new fruit plants collection-exposition when reconstructing the area of old plantings.
金美味苹果基因组的核苷酸序列位于 GenBank 数据库中，根据该序列，对限制四核苷酸和六核苷酸重复区域的 SSR 类型分子标记进行了硅设计。筛选出的信息量最大的标记在毛细管电泳中显示出清晰的峰值，可以揭示苹果基因组某些区域的高度多态性。所开发的 SSR 标记可有效用于苹果品种的遗传多样性鉴定和 DNA 鉴定，并建立品种对应关系。这些标记被用于分析白俄罗斯国家科学院中央植物园（CBG）老果园中苹果树的遗传多样性。结果表明，园中生长的苹果树在基因上与当地的古老品种以及个别外国品种十分接近。在 CBG 老果园的树木中，有 Antonovka obyknovennaya、Minskoe、Papirovka.Wealthy、Melba、Bogatyr'。所取得的成果将用于保存对国家有价值的水果作物（苹果树）的遗传资源，以及在重建老种植区时制定新的水果植物收集-展示设计项目。
{"title":"Genetic diversity of apple trees in the old orchard of the Central Botanical Garden of the National Academy оf Sciences of Belarus","authors":"A. A. Famina, A. N. Zainchkovskaya, Р. V. Kuzmitskaya, О. Y. Urbanovich, Р. А. Pashkevich, L. S. Sidor, В. Y. Anoshenko, L. V. Goncharova","doi":"10.29235/1029-8940-2024-69-1-57-67","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-57-67","url":null,"abstract":"Based on the sequence of the Golden Delicious apple genome, whose nucleotide sequence is located in the GenBank database, the design of molecular markers of SSR type that limit the area of tetra- and hexanucleotide repeats was carried out in silico. The most informative of them were selected, which showed clear peaks on capillary electrophoresis and made it possible to reveal a high level of polymorphism in certain apple genome regions. The developed SSR markers can be effectively used for the genetic diversity assession and DNA identification of apple varieties, establishment of the varietal correspondence. These markers were used to analyze the genetic diversity of apple trees in the old orchard of the Central Botanical Garden of the National Academy of Sciences of Belarus (CBG) where trees were planted from 1932 to 1940. It is shown that the trees growing in the garden are genetically close to old local varieties, as well as to individual foreign ones. It is established that among the trees of the CBG old orchard there are such varieties as Antonovka obyknovennaya, Minskoe, Papirovka. Wealthy, Melba, Bogatyr’. The obtained results will be used to save the genetic resources of the fruit crop valuable for the country, which is an apple tree, as well as to develop a design project of a new fruit plants collection-exposition when reconstructing the area of old plantings.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":" 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139792937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-08DOI: 10.29235/1029-8940-2024-69-1-47-56
J. V. Korzun, A. A. Kulikova
The aim of the work is to study the influence of factors of abiotic and biotic origin on abundance, growth rate and development of amphibian larvae in natural conditions.The scientific novelty of the work lies in studying the degree of interaction of amphibian larvae with each other and with invertebrate predators, their interaction in natural conditions. The main part of the work contains a literature review, the work methodology and the result description.In conclusion, it was noted that under natural conditions, the magnitude of impact of predators on the number of amphibian larvae is much lower than it has been previously thought; they are not able to provide a significant function of regulating the number of tadpoles. Abiotic environmental factors, being within the optimum limits, do not affect the mortality of larvae, and food in natural water bodies is not a factor limiting abundance. The most intensive death of larvae occurs in the early larval development stage. Despite a low overall density of larvae in a natural water body, most amphibian larvae are periodically exposed to metabolites and are affected by the “group effect”. This is expressed in the inhibition of growth and development of individuals, the death or decrease in the decline in the viability of the most sensitive individuals, and also increases the likelihood of their death from other factors.
{"title":"Dynamics of change in the number of amphibian larvae in pond conditions and factors determining it in the central part of Belarus","authors":"J. V. Korzun, A. A. Kulikova","doi":"10.29235/1029-8940-2024-69-1-47-56","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-47-56","url":null,"abstract":"The aim of the work is to study the influence of factors of abiotic and biotic origin on abundance, growth rate and development of amphibian larvae in natural conditions.The scientific novelty of the work lies in studying the degree of interaction of amphibian larvae with each other and with invertebrate predators, their interaction in natural conditions. The main part of the work contains a literature review, the work methodology and the result description.In conclusion, it was noted that under natural conditions, the magnitude of impact of predators on the number of amphibian larvae is much lower than it has been previously thought; they are not able to provide a significant function of regulating the number of tadpoles. Abiotic environmental factors, being within the optimum limits, do not affect the mortality of larvae, and food in natural water bodies is not a factor limiting abundance. The most intensive death of larvae occurs in the early larval development stage. Despite a low overall density of larvae in a natural water body, most amphibian larvae are periodically exposed to metabolites and are affected by the “group effect”. This is expressed in the inhibition of growth and development of individuals, the death or decrease in the decline in the viability of the most sensitive individuals, and also increases the likelihood of their death from other factors.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":" 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139793445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-08DOI: 10.29235/1029-8940-2024-69-1-79-88
N. A. Kovalenko, G. N. Supichenkо, Т. I. Ahramovich, Y. A. Niachai, V. N. Leontiev
The steam distillation method was used to obtain samples of essential oil of cultivars with a columnar and spherical shape of the crown Thuja occidentalis introduced in the Republic of Belarus. More than 26 components have been identified and determined in the essential oil of Thuja occidentalis by gas-liquid chromatography. The dominant components are α-thujone (∼55–60 %), β-thujone (∼10–12 %), fenchone (∼8–16 %), sabinene (∼1–5 %), camphor (∼1–4 %), bornylacetate (∼1–3 %). Distributions of terpenoid enantiomers in the essential oil of Thuja occidentalis cultivars are shown. The antimicrobial activity of Thuja occidentalis essential oils was detected in relation to test cultures of gram-positive and gram-negative bacteria. Bacterial cultures were more sensitive to the inhibitory effect of essential oil samples from plants with a spherical crown shape. The influence of the optical activity of camphor on the essential oil antimicrobial properties is shown. It has been established that alcohol extracts of Thuja occidentalis are inferior to essential oils in terms of antimicrobial activity. The effect of the crown shape and the color of the studied cultivar needles on their antioxidant properties is shown.
{"title":"Antimicrobial activity of Thuja occidentalis essential oils and extracts","authors":"N. A. Kovalenko, G. N. Supichenkо, Т. I. Ahramovich, Y. A. Niachai, V. N. Leontiev","doi":"10.29235/1029-8940-2024-69-1-79-88","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-79-88","url":null,"abstract":"The steam distillation method was used to obtain samples of essential oil of cultivars with a columnar and spherical shape of the crown Thuja occidentalis introduced in the Republic of Belarus. More than 26 components have been identified and determined in the essential oil of Thuja occidentalis by gas-liquid chromatography. The dominant components are α-thujone (∼55–60 %), β-thujone (∼10–12 %), fenchone (∼8–16 %), sabinene (∼1–5 %), camphor (∼1–4 %), bornylacetate (∼1–3 %). Distributions of terpenoid enantiomers in the essential oil of Thuja occidentalis cultivars are shown. The antimicrobial activity of Thuja occidentalis essential oils was detected in relation to test cultures of gram-positive and gram-negative bacteria. Bacterial cultures were more sensitive to the inhibitory effect of essential oil samples from plants with a spherical crown shape. The influence of the optical activity of camphor on the essential oil antimicrobial properties is shown. It has been established that alcohol extracts of Thuja occidentalis are inferior to essential oils in terms of antimicrobial activity. The effect of the crown shape and the color of the studied cultivar needles on their antioxidant properties is shown.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":"8 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139851280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-30DOI: 10.29235/1029-8940-2023-68-3-257-264
Yu. I. Okhremenko, H. Gajduchenko
A scheme for measuring the plastic features of the brown bullhead, including 28 morphometric characteristics, has been proposed. Based on the cluster analysis of morphometric characteristics, the size groups of species of a model water body were determined (Karpin village pond, Maloritsky district, Brest region).The change in the exterior parameters of the brown bullhead during its ontogenesis has been shown for the first time. It is proposed to divide brown bullhead individuals into 3 size groups for analysis: A – young individuals not involved in reproduction (body length without a caudal fin up to 135.0 mm); B – mature individuals that have started breeding (sizes from 136.0 to 174.0 mm); C – adults, actively breeding individuals (sizes from 175.0 to 187.0 mm).
提出了一种测量褐头鱼塑性特征的方案，包括28个形态特征。基于形态计量学特征聚类分析，确定了一个模型水体(Brest地区Maloritsky区的Karpin village pond)的物种大小群。首次研究了褐头鱼个体发生过程中外部参数的变化。建议将褐头鱼个体分为3个大小组进行分析:A -不参与繁殖的幼鱼个体(无尾鳍体长可达135.0 mm);B -已开始繁殖的成熟个体(体长136.0至174.0毫米);C -成年，积极繁殖个体(大小从175.0到187.0毫米)。
{"title":"Variability of plastic features of size groups of the american catfish Ameiurus nebulosus (Lesueur, 1819) – alien species in the water bodies of Belarus","authors":"Yu. I. Okhremenko, H. Gajduchenko","doi":"10.29235/1029-8940-2023-68-3-257-264","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-3-257-264","url":null,"abstract":"A scheme for measuring the plastic features of the brown bullhead, including 28 morphometric characteristics, has been proposed. Based on the cluster analysis of morphometric characteristics, the size groups of species of a model water body were determined (Karpin village pond, Maloritsky district, Brest region).The change in the exterior parameters of the brown bullhead during its ontogenesis has been shown for the first time. It is proposed to divide brown bullhead individuals into 3 size groups for analysis: A – young individuals not involved in reproduction (body length without a caudal fin up to 135.0 mm); B – mature individuals that have started breeding (sizes from 136.0 to 174.0 mm); C – adults, actively breeding individuals (sizes from 175.0 to 187.0 mm).","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84353734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-30DOI: 10.29235/1029-8940-2023-68-3-224-233
H. Bukliarevich, M. Titok
It was found that the synthesis of surfactants by R. pyridinivorans 5Ap bacteria can be increased by exposing them to a short temperature shock (55 °C for 20 minutes) after 24 hour cultivation in a minimal medium containing molasses (3 %) and hexadecane (2 %) (9 % increase in the emulsification index). Gene activation encoding global regulators of cell metabolism, including those performing a protective function under stress, was recorded in this cultivation mode. In particular, the mRNA amount determining the synthesis of the alternative transcription factor SigH increased 90.8 times and containing in the promoters its binding sites fmdB cochaperone – 59.3 times, hsp22.5 chaperone – 81.1 times, and the trxB gene encoding thioredoxin reductase – 73.1 times. In addition, it was shown that the transcriptional activation of groEL1, groEL2 and dnaJ genes determining the synthesis of heat shock proteins was 2.2, 2.6 and 4.4 times, respectively. The obtained results suggest that an increase in the alternative factor sigma H synthesis, which activates protective cellular metabolism, as well as structural, heat shock proteins under short temperature stress, leads to an increased production of surfactants, which can be used to optimize the synthesis of these secondary metabolites for biotechnological use.
{"title":"Influence of a temperature shock on the synthesis efficiency of surfactants by Rhodococcus pyridinivorans 5Ap bacteria","authors":"H. Bukliarevich, M. Titok","doi":"10.29235/1029-8940-2023-68-3-224-233","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-3-224-233","url":null,"abstract":"It was found that the synthesis of surfactants by R. pyridinivorans 5Ap bacteria can be increased by exposing them to a short temperature shock (55 °C for 20 minutes) after 24 hour cultivation in a minimal medium containing molasses (3 %) and hexadecane (2 %) (9 % increase in the emulsification index). Gene activation encoding global regulators of cell metabolism, including those performing a protective function under stress, was recorded in this cultivation mode. In particular, the mRNA amount determining the synthesis of the alternative transcription factor SigH increased 90.8 times and containing in the promoters its binding sites fmdB cochaperone – 59.3 times, hsp22.5 chaperone – 81.1 times, and the trxB gene encoding thioredoxin reductase – 73.1 times. In addition, it was shown that the transcriptional activation of groEL1, groEL2 and dnaJ genes determining the synthesis of heat shock proteins was 2.2, 2.6 and 4.4 times, respectively. The obtained results suggest that an increase in the alternative factor sigma H synthesis, which activates protective cellular metabolism, as well as structural, heat shock proteins under short temperature stress, leads to an increased production of surfactants, which can be used to optimize the synthesis of these secondary metabolites for biotechnological use.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":"23 Suppl 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80334163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-29DOI: 10.29235/1029-8940-2023-68-3-183-196
A. A. Akunevich, V. V. Khrustalev, T. Khrustaleva, L. Kordyukova, A. Arutyunyan
The epidermal growth factor (EGF) is a peptide of the EGF-like growth factor family with a common conserved EGF-like domain formed by three intramolecular disulfide bonds. This article describes changes in the spatial structure of EGF and its mutant form with the D46G substitution in its C-terminal fragment observed upon disulfide bonds reduction in the corresponding synthetic peptides in a 0.01 M phosphate buffer (pH = 7.4). The structure was analyzed using circular dichroism spectroscopy, spectrofluorimetry, native polyacrylamide gel electrophoresis, and centrifugal ultrafiltration. It was shown that disulfide bonds reduction changes the geometry of the EGF-like domain towards an increase in the content of the beta-structure, while these peptides remain in dimeric form. According to the molecular modeling results, this can lead to the elongation of the main beta-hairpin of the EGF-like domain, to the elongation of the intermolecular beta-structure, or to the formation of a new beta-structure between the N- and C-terminal fragments of each molecule, which will change the intermolecular interface in dimeric form. Disulfide bonds reduction prevents EGF dimer dissociation to monomers. Under physiological conditions, this can lead to the inability of EGF to form binding sites for EGFR (epidermal growth factor receptor) and to cause its activation.
{"title":"The role of disulfide bonds in the formation of the spatial structure of the human epidermal growth factor","authors":"A. A. Akunevich, V. V. Khrustalev, T. Khrustaleva, L. Kordyukova, A. Arutyunyan","doi":"10.29235/1029-8940-2023-68-3-183-196","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-3-183-196","url":null,"abstract":"The epidermal growth factor (EGF) is a peptide of the EGF-like growth factor family with a common conserved EGF-like domain formed by three intramolecular disulfide bonds. This article describes changes in the spatial structure of EGF and its mutant form with the D46G substitution in its C-terminal fragment observed upon disulfide bonds reduction in the corresponding synthetic peptides in a 0.01 M phosphate buffer (pH = 7.4). The structure was analyzed using circular dichroism spectroscopy, spectrofluorimetry, native polyacrylamide gel electrophoresis, and centrifugal ultrafiltration. It was shown that disulfide bonds reduction changes the geometry of the EGF-like domain towards an increase in the content of the beta-structure, while these peptides remain in dimeric form. According to the molecular modeling results, this can lead to the elongation of the main beta-hairpin of the EGF-like domain, to the elongation of the intermolecular beta-structure, or to the formation of a new beta-structure between the N- and C-terminal fragments of each molecule, which will change the intermolecular interface in dimeric form. Disulfide bonds reduction prevents EGF dimer dissociation to monomers. Under physiological conditions, this can lead to the inability of EGF to form binding sites for EGFR (epidermal growth factor receptor) and to cause its activation.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":"PP 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84295922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-29DOI: 10.29235/1029-8940-2023-68-3-197-205
U. Gayibov, H. Ruziboev, M. Y. Eraliev, M. Pozilov, M. Asrarov, N. Abdulladjanova, Ch. Xiandan, A. Sysa
The state of lipid peroxidation (LPO), respiration and oxidative phosphorylation, mitochondrial permeability transition pore (mPTP), and the antiradical activity of rat liver mitochondria in the streptozotocin (STZ)-induced diabetes condition were studied with the consideration of the ways of correcting detected membrane damages using gossitan isolated from the cotton plant Gossypium hirsutum L. It was shown that the rate of respiration of liver mitochondria in states V3 and V4 increases during STZ-induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The findings suggest that the uncoupling of respiration and oxidative phosphorylation takes place during STZ-induced diabetes. It was shown that in the STZ-induced diabetes condition, the rate of swelling of rat liver mitochondria is higher than that of the healthy ones; this means that mPTP of rat liver mitochondria is in the open state. Gossitan recovers mPTP to the normal condition, thereby removing the STZ effect on mitochondria. Gossitan (a personal dose is 10 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver mitochondria, probably due to its antioxidant properties.
{"title":"Physiological changes of mitochondria under the diabetes condition and pharmacological approaches to eliminate them","authors":"U. Gayibov, H. Ruziboev, M. Y. Eraliev, M. Pozilov, M. Asrarov, N. Abdulladjanova, Ch. Xiandan, A. Sysa","doi":"10.29235/1029-8940-2023-68-3-197-205","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-3-197-205","url":null,"abstract":"The state of lipid peroxidation (LPO), respiration and oxidative phosphorylation, mitochondrial permeability transition pore (mPTP), and the antiradical activity of rat liver mitochondria in the streptozotocin (STZ)-induced diabetes condition were studied with the consideration of the ways of correcting detected membrane damages using gossitan isolated from the cotton plant Gossypium hirsutum L. It was shown that the rate of respiration of liver mitochondria in states V3 and V4 increases during STZ-induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The findings suggest that the uncoupling of respiration and oxidative phosphorylation takes place during STZ-induced diabetes. It was shown that in the STZ-induced diabetes condition, the rate of swelling of rat liver mitochondria is higher than that of the healthy ones; this means that mPTP of rat liver mitochondria is in the open state. Gossitan recovers mPTP to the normal condition, thereby removing the STZ effect on mitochondria. Gossitan (a personal dose is 10 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver mitochondria, probably due to its antioxidant properties.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":"168 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78069299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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