Pub Date : 2022-05-05DOI: 10.29235/1029-8940-2022-67-2-243-256
Ya. K. Kulikou
The possibility of using the polyfunctional properties of plant-microbial symbiosis in the development of resourcesaving technologies in crop production has been substantiated. An ecological assessment of the interaction of legume genotypes with rhizosphere microflora was carried out, and the role of plant-microbial systems in the accumulation of biological nitrogen was shown. The positive effect of plant-microbial interactions on soil biogenicity and plant adaptation to biotic stresses was revealed.
{"title":"Ecological functions of plant-microbial symbioses and their role in the development of resource-saving biotechnologies","authors":"Ya. K. Kulikou","doi":"10.29235/1029-8940-2022-67-2-243-256","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-243-256","url":null,"abstract":"The possibility of using the polyfunctional properties of plant-microbial symbiosis in the development of resourcesaving technologies in crop production has been substantiated. An ecological assessment of the interaction of legume genotypes with rhizosphere microflora was carried out, and the role of plant-microbial systems in the accumulation of biological nitrogen was shown. The positive effect of plant-microbial interactions on soil biogenicity and plant adaptation to biotic stresses was revealed.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88798010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.29235/1029-8940-2022-67-2-190-196
T. A. Pilipchuk, A. Akhremchuk, E. I. Kаlаmiуets
Full nucleotide sequence of bacteriophage Pseudomonas phage BIM BV-45 D – active component of biopesticide Multiphage intended for control of crop bacterial diseases caused by Pseudomonas syringae was analyzed. It was found that phage genome is represented by linear double-stranded DNA sized 40383 b. p. (average GC contents equals 58 %), comprising 46 open reading frames, including 13 described in genomes of closely related phages. 4 control sequences typical for bacterial genes recognized by sigma factor (σ70) of RNA polymerase were detected, specific phage promoters were not localized. The identity was established of the majority of protein amino acid sequences of the Pseudomonas phage BIM BV-45 D with the proteins of the known Pseudomonas phage Andromeda (95‒100 %), at the same time, the sequences of the DNA endonuclease protein (gene 22) are similar (63 %) to the corresponding protein of Pseudomonas phage PollyC. The obtained data suggest that the mosaic structure of Pseudomonas phage BIM BV-45 D genome is due to recombinant rearrangements between the afore-mentioned phages.
{"title":"Molecular-genetic structure of Pseudomonas phage BIM BV-45 D","authors":"T. A. Pilipchuk, A. Akhremchuk, E. I. Kаlаmiуets","doi":"10.29235/1029-8940-2022-67-2-190-196","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-190-196","url":null,"abstract":"Full nucleotide sequence of bacteriophage Pseudomonas phage BIM BV-45 D – active component of biopesticide Multiphage intended for control of crop bacterial diseases caused by Pseudomonas syringae was analyzed. It was found that phage genome is represented by linear double-stranded DNA sized 40383 b. p. (average GC contents equals 58 %), comprising 46 open reading frames, including 13 described in genomes of closely related phages. 4 control sequences typical for bacterial genes recognized by sigma factor (σ70) of RNA polymerase were detected, specific phage promoters were not localized. The identity was established of the majority of protein amino acid sequences of the Pseudomonas phage BIM BV-45 D with the proteins of the known Pseudomonas phage Andromeda (95‒100 %), at the same time, the sequences of the DNA endonuclease protein (gene 22) are similar (63 %) to the corresponding protein of Pseudomonas phage PollyC. The obtained data suggest that the mosaic structure of Pseudomonas phage BIM BV-45 D genome is due to recombinant rearrangements between the afore-mentioned phages.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91112662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.29235/1029-8940-2022-67-2-206-218
N. Pavlovskiy
Based on the results of a 13-year study of the yield of 20 cultivars of highbush blueberry and 3 cultivars of half-highbush blueberry, introduced in Belarus from different geographical regions of the world, various indicators of adaptability were calculated. It has been established that tall blueberry cultivars are characterized by significant yield variability. The prevailing contribution to the overall yield variability of blueberry cultivars (45.9 %) was made by the interaction of factors “cultivar × year”. High-yielding and yielding cultivars of blueberries are characterized by greater genetic and ecological plasticity, but weak stress resistance and low stability. According to the set of adaptability indicators, the assessed blueberry cultivars are classified into 3 groups: 1) high-yielding and yielding cultivars, plastic, but unstable fruiting; 2) medium-yielding cultivars, stably fruiting, characterized by low and medium plasticity; 3) low-yielding cultivars unsuitable for industrial cultivation in Belarus.
{"title":"Evaluation of ecological adaptability of blueberry cultivars introduced in Belarus","authors":"N. Pavlovskiy","doi":"10.29235/1029-8940-2022-67-2-206-218","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-206-218","url":null,"abstract":"Based on the results of a 13-year study of the yield of 20 cultivars of highbush blueberry and 3 cultivars of half-highbush blueberry, introduced in Belarus from different geographical regions of the world, various indicators of adaptability were calculated. It has been established that tall blueberry cultivars are characterized by significant yield variability. The prevailing contribution to the overall yield variability of blueberry cultivars (45.9 %) was made by the interaction of factors “cultivar × year”. High-yielding and yielding cultivars of blueberries are characterized by greater genetic and ecological plasticity, but weak stress resistance and low stability. According to the set of adaptability indicators, the assessed blueberry cultivars are classified into 3 groups: 1) high-yielding and yielding cultivars, plastic, but unstable fruiting; 2) medium-yielding cultivars, stably fruiting, characterized by low and medium plasticity; 3) low-yielding cultivars unsuitable for industrial cultivation in Belarus.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91000928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.29235/1029-8940-2022-67-2-197-205
I. V. Nikalayeva, V. Sheibak, E. Doroshenko
It is known that the initiating event of liver damage during lead intoxication is the effect of lead on the intestinal microbiome and the metabolic profile of enterocytes. The aim of the study was to reveal the dependence of the concentrations of free amino acids and their derivatives in the liver on the amino acid pool of the microbial-tissue complex of the small intestine. We used rats weighing 120‒140 g, which were injected intragastrically for three weeks with drinking water with lead acetate. Free amino acids and their nitrogen-containing metabolites in the microbial-tissue complex of the intestine and liver were determined by HPLC. Enteral intake of lead acetate with drinking water changes the profile of free amino acids and their derivatives in the microbial-tissue complex of the small intestine, which correlates with a violation of amino acid homeostasis in the liver. The analysis of the correlations of the indicators we determined indicates the essential role of nitrogen-containing metabolites of amino acids ‒ ethanolamine and phosphoethanolamine, taurine, and cystathionine. The direction of the correlation relationship between various nitrogen-containing metabolites of the microbial-tissue complex and the liver can be considered as marker of discoordination of homeostasis.
{"title":"Amino acid balance of microbial-tissue complex in the small intestinal and liver under administration of lead acetate","authors":"I. V. Nikalayeva, V. Sheibak, E. Doroshenko","doi":"10.29235/1029-8940-2022-67-2-197-205","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-197-205","url":null,"abstract":"It is known that the initiating event of liver damage during lead intoxication is the effect of lead on the intestinal microbiome and the metabolic profile of enterocytes. The aim of the study was to reveal the dependence of the concentrations of free amino acids and their derivatives in the liver on the amino acid pool of the microbial-tissue complex of the small intestine. We used rats weighing 120‒140 g, which were injected intragastrically for three weeks with drinking water with lead acetate. Free amino acids and their nitrogen-containing metabolites in the microbial-tissue complex of the intestine and liver were determined by HPLC. Enteral intake of lead acetate with drinking water changes the profile of free amino acids and their derivatives in the microbial-tissue complex of the small intestine, which correlates with a violation of amino acid homeostasis in the liver. The analysis of the correlations of the indicators we determined indicates the essential role of nitrogen-containing metabolites of amino acids ‒ ethanolamine and phosphoethanolamine, taurine, and cystathionine. The direction of the correlation relationship between various nitrogen-containing metabolites of the microbial-tissue complex and the liver can be considered as marker of discoordination of homeostasis.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87327686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.29235/1029-8940-2022-67-2-172-180
P. Y. Lobanovskaya, L. Akimova, E. Kheidorova
The material was collected during the period 2018‒2021 by dissection of internal organs of European bisons, red deers, European roe deers and elks. The found helminths were identified morphologically and using molecular genetic tools. Analysis of 16 ND4 mDNA sequences of A. sidemi revealed low level of genetic diversity which may indicate that Ashworthius sidemi in Belarus spread from one source.
{"title":"Distribution and characteristics of Ashworthius sidemi Schulz, 1933 (Nematoda, Trichostrongylidae) in wild ungulates in Belarus","authors":"P. Y. Lobanovskaya, L. Akimova, E. Kheidorova","doi":"10.29235/1029-8940-2022-67-2-172-180","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-172-180","url":null,"abstract":"The material was collected during the period 2018‒2021 by dissection of internal organs of European bisons, red deers, European roe deers and elks. The found helminths were identified morphologically and using molecular genetic tools. Analysis of 16 ND4 mDNA sequences of A. sidemi revealed low level of genetic diversity which may indicate that Ashworthius sidemi in Belarus spread from one source.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81320994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.29235/1029-8940-2022-67-2-158-171
M. Lushchyk, M. Ameliyanovich, H. Tuzava, I. Mosse, L. Danilova
The article discusses the prospects for studying polymorphic variants of peroxisome proliferator-activated receptor genes (PPARs) of three types (PPARα, PPARδ, and PPARγ) in diabetes mellitus (DM), taking into account their key role in the regulation of energy homeostasis, production of pro-inflammatory cytokines, and lipid characteristics and glycemia control. The main emphasis is on the use of screening methods for testing patients for carriage of single nucleotide polymorphisms (SNPs) in order to improve approaches to identifying risk groups for the formation of DM and associated diseases, and subsequent personification of corrective measures. The clinical, laboratory and molecular genetic characteristics of groups of patients with type 1 and 2 diabetes, healthy volunteers are presented. The prevalence of SNPs in the genes of receptors activated by the peroxisome proliferator in patients with DM was studied in comparison with the control group. Among the evaluated SNPs of the rs135551 gene, PPARA showed the clearest association with the presence of DM. Four variants of haplotypes highly associated with DM1 and DM2 were identified. The expediency of further clarification of the clinical and genetic heterogeneity of cases of diabetes within the DM1 and DM2 groups is discussed. The prospects of this direction for the development of preventive technologies in diabetology, long-term epidemiological molecular genetic screenings are assessed.
{"title":"Molecular-genetic characteristics of patients with diabetes mellitus. Vestsi Natsyyanal’nai akademii navuk Belarusi","authors":"M. Lushchyk, M. Ameliyanovich, H. Tuzava, I. Mosse, L. Danilova","doi":"10.29235/1029-8940-2022-67-2-158-171","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-158-171","url":null,"abstract":"The article discusses the prospects for studying polymorphic variants of peroxisome proliferator-activated receptor genes (PPARs) of three types (PPARα, PPARδ, and PPARγ) in diabetes mellitus (DM), taking into account their key role in the regulation of energy homeostasis, production of pro-inflammatory cytokines, and lipid characteristics and glycemia control. The main emphasis is on the use of screening methods for testing patients for carriage of single nucleotide polymorphisms (SNPs) in order to improve approaches to identifying risk groups for the formation of DM and associated diseases, and subsequent personification of corrective measures. The clinical, laboratory and molecular genetic characteristics of groups of patients with type 1 and 2 diabetes, healthy volunteers are presented. The prevalence of SNPs in the genes of receptors activated by the peroxisome proliferator in patients with DM was studied in comparison with the control group. Among the evaluated SNPs of the rs135551 gene, PPARA showed the clearest association with the presence of DM. Four variants of haplotypes highly associated with DM1 and DM2 were identified. The expediency of further clarification of the clinical and genetic heterogeneity of cases of diabetes within the DM1 and DM2 groups is discussed. The prospects of this direction for the development of preventive technologies in diabetology, long-term epidemiological molecular genetic screenings are assessed.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79133099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.29235/1029-8940-2022-67-2-135-146
N. G. Averina, S. M. Savina, I. Dremuk, H. Yemelyanava, Yu. V. Pryshchepchyk, А. V. Usatov
Physiological and biochemical characteristics of two varieties of winter wheat seedlings with red (Et W5) and green (Vladi) coleoptiles have been studied. Variety Et W5 with high content of anthocyanins (on average 412 ± 52 μmol/g fr wt), chlorophylls (Chl) and carotenoids and a low level of heme and proline in coleoptiles had high frost resistance (88 % of surviving plants exposed to temperatures of ‒8 °C for 5 h at the age of 7 days) and a high ability to continue growth and development after undergoing cooling. Variety Vladi with a low content of anthocyanins (on average 12.5 ± 1.2 μmol/g fr wt), Chl and carotenoids, but a higher level of heme and proline in coleoptiles compared to varieties Et W5 had low level of frost resistance (80 % of surviving plants) and was characterized by a low ability to continue growth and development after undergoing cooling. Soaking wheat seeds in a solution of exogenous ALA (50 mg/l) for 2 h before sowing increased the germination of seeds of both varieties, enhanced the length of plants of the Et W5 variety by 10 %, 1.4 times the content of anthocyanins in the coleoptiles of this variety plants and also increased their frost resistance up to 97 %. Under action of ALA frost resistance of Vladi seedlings increased up to 93 %.
{"title":"Influence of 5-aminolevulinic acid on physiological and biochemical characteristics of winter wheat varieties with different levels of anthocyanins in coleoptiles","authors":"N. G. Averina, S. M. Savina, I. Dremuk, H. Yemelyanava, Yu. V. Pryshchepchyk, А. V. Usatov","doi":"10.29235/1029-8940-2022-67-2-135-146","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-135-146","url":null,"abstract":"Physiological and biochemical characteristics of two varieties of winter wheat seedlings with red (Et W5) and green (Vladi) coleoptiles have been studied. Variety Et W5 with high content of anthocyanins (on average 412 ± 52 μmol/g fr wt), chlorophylls (Chl) and carotenoids and a low level of heme and proline in coleoptiles had high frost resistance (88 % of surviving plants exposed to temperatures of ‒8 °C for 5 h at the age of 7 days) and a high ability to continue growth and development after undergoing cooling. Variety Vladi with a low content of anthocyanins (on average 12.5 ± 1.2 μmol/g fr wt), Chl and carotenoids, but a higher level of heme and proline in coleoptiles compared to varieties Et W5 had low level of frost resistance (80 % of surviving plants) and was characterized by a low ability to continue growth and development after undergoing cooling. Soaking wheat seeds in a solution of exogenous ALA (50 mg/l) for 2 h before sowing increased the germination of seeds of both varieties, enhanced the length of plants of the Et W5 variety by 10 %, 1.4 times the content of anthocyanins in the coleoptiles of this variety plants and also increased their frost resistance up to 97 %. Under action of ALA frost resistance of Vladi seedlings increased up to 93 %.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81773735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.29235/1029-8940-2022-67-2-181-189
Ya. V. Khamiuk, R. Litvinovskaya, A. Kolbas
A step-by-step study of the biological activity of 24-epibrassinolide (EB) and its sodium salts of esters with sulfuric acid (sodium 2-, 3-, 22and 23-monosulfates and disodium 2.3-disulfate EB) was carried out in experiments on the effect on the growth parameters and crop yield of annual sunflower Helianthus annuus L. (Helios variety) depending on growing conditions. In experiments on a groundless medium, the effect of epibrassinolide sulfuric acid esters on primary plant growth at concentrations of 10–11–10–9 М turned out to be insignificant (with the exception of disodium 2.3–disulfate EB), to a greater extent the activity of the studied compounds was marked in laboratory experiment in soil culture. The greatest effect on growth and development plant was noted in field experiments. It is shown that the most effective and universal compound according to the studied parameters for two types of treatment (soaking seeds and spraying plants) is disodium 2.3-disulfate EB.
通过不同生长条件对一年生向日葵Helianthus annuus L. (Helios品种)生长参数和产量的影响，逐步研究了24-表油菜素内酯(EB)及其酯类钠盐(2-、3-、22 -、23-单硫酸钠和2.3-二硫酸二钠EB)与硫酸的生物活性。在无底培养基上的实验中，表油菜素内酯硫酸酯在10-11-10-9 М浓度下对初生植物生长的影响不显著(2.3 -二硫酸二钠EB除外)，所研究化合物的活性在更大程度上是在土壤培养的实验室实验中被标记出来的。田间试验表明对植株生长发育影响最大。结果表明，在浸种和喷施两种处理方法中，最有效、最通用的药剂是2.3-二硫酸钠EB。
{"title":"The effect of epibrassinolide and its sulfuric acid esters on the morphometric parameters of sunflower Helianthus annuus L. (cv. Helios)","authors":"Ya. V. Khamiuk, R. Litvinovskaya, A. Kolbas","doi":"10.29235/1029-8940-2022-67-2-181-189","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-181-189","url":null,"abstract":"A step-by-step study of the biological activity of 24-epibrassinolide (EB) and its sodium salts of esters with sulfuric acid (sodium 2-, 3-, 22and 23-monosulfates and disodium 2.3-disulfate EB) was carried out in experiments on the effect on the growth parameters and crop yield of annual sunflower Helianthus annuus L. (Helios variety) depending on growing conditions. In experiments on a groundless medium, the effect of epibrassinolide sulfuric acid esters on primary plant growth at concentrations of 10–11–10–9 М turned out to be insignificant (with the exception of disodium 2.3–disulfate EB), to a greater extent the activity of the studied compounds was marked in laboratory experiment in soil culture. The greatest effect on growth and development plant was noted in field experiments. It is shown that the most effective and universal compound according to the studied parameters for two types of treatment (soaking seeds and spraying plants) is disodium 2.3-disulfate EB.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78993603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.29235/1029-8940-2022-67-2-229-242
I. Volotovski, S. Pinchuk
The given review is an attempt of concentrated consideration of flow cytometry problem which is widely used as a fundamental research approach in various fields of biology like cell biology, biophysics, biochemistry and molecular biology and also in applied and diagnostic medicine. The method principle, construction of flow cytometers and their possibilities (study of structure and function state of cell populations and cell sorting), usage of lasers in flow cytometers, wide assortment of fluorophores and monoclonal antibodies. The concrete examples of flow cytometer methods in different experiments are given. The trends in development of flow cytometry are considered.
{"title":"Flow cytometry. Basics of technology and its application in biology","authors":"I. Volotovski, S. Pinchuk","doi":"10.29235/1029-8940-2022-67-2-229-242","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-229-242","url":null,"abstract":"The given review is an attempt of concentrated consideration of flow cytometry problem which is widely used as a fundamental research approach in various fields of biology like cell biology, biophysics, biochemistry and molecular biology and also in applied and diagnostic medicine. The method principle, construction of flow cytometers and their possibilities (study of structure and function state of cell populations and cell sorting), usage of lasers in flow cytometers, wide assortment of fluorophores and monoclonal antibodies. The concrete examples of flow cytometer methods in different experiments are given. The trends in development of flow cytometry are considered.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82880547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.29235/1029-8940-2022-67-2-147-157
O. V. Chizhik, A. N. Yukhimuk, V. Reshetnikov
The Vacciniaceae family introduction carry out by the Central Botanical Garden of the National Academy of Sciences of Belarus since 1980. It made it possible to give rise the new direction for the republic – the industrial berry growing. To increase the efficiency of high-quality planting material obtaining it is necessary to study the fundamental processes underlying the growth and development of plants and to establish the possible ways of their regulation. It is of current interest to confirm the conformity of the planting material to the declared variety (the genetic passports elaboration). The biotechnology development promotes a new approaches and technologies for studying and analyzing of a plant organism genome and proteome. For the first time the molecular genetic identification for Vaccinium corymbosum L. varieties were carried out by Start Codon Targeted (SCoT) system. This method of DNA certification makes it possible to check the cultivar conformity of the blueberry planting material. For the first time by 2D electrophoresis the proteomic maps of leaf tissue were obtained and the proteomic status of four highbush blueberry varieties – in vitro culture and parental plants (in vivo) was estimated. The species and varieties specificity of the expressed genome products were established. The proteins that claims to be markers for the Vaccinium species and the variety markers were identified for the first time, as well as the physiological state markers of high blueberry cultivated in vivo and in vitro.
{"title":"Highbush blueberry (Vaccinium corymbosum L.): proteomic and molecular genetic studies. Vestsi Natsyyanal’nai akademii navuk Belarusi","authors":"O. V. Chizhik, A. N. Yukhimuk, V. Reshetnikov","doi":"10.29235/1029-8940-2022-67-2-147-157","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-147-157","url":null,"abstract":"The Vacciniaceae family introduction carry out by the Central Botanical Garden of the National Academy of Sciences of Belarus since 1980. It made it possible to give rise the new direction for the republic – the industrial berry growing. To increase the efficiency of high-quality planting material obtaining it is necessary to study the fundamental processes underlying the growth and development of plants and to establish the possible ways of their regulation. It is of current interest to confirm the conformity of the planting material to the declared variety (the genetic passports elaboration). The biotechnology development promotes a new approaches and technologies for studying and analyzing of a plant organism genome and proteome. For the first time the molecular genetic identification for Vaccinium corymbosum L. varieties were carried out by Start Codon Targeted (SCoT) system. This method of DNA certification makes it possible to check the cultivar conformity of the blueberry planting material. For the first time by 2D electrophoresis the proteomic maps of leaf tissue were obtained and the proteomic status of four highbush blueberry varieties – in vitro culture and parental plants (in vivo) was estimated. The species and varieties specificity of the expressed genome products were established. The proteins that claims to be markers for the Vaccinium species and the variety markers were identified for the first time, as well as the physiological state markers of high blueberry cultivated in vivo and in vitro.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89799429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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