Process Biochemistry最新文献_第4页

Pathogen-targeting bismuth nanocluster via photothermally-mediated NDM-1 inactivation and bacterial membrane destabilization combat NDM-1-producing bacteria

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.011

Xichan He , Shaobing Liu , Jianan Tong , Yuhui Qiao , Xue Li , Kaixuan Dong , Dongsheng Yu , Yanshu Gu

New Delhi metallo-β-lactamase-1 (NDM-1) is the most prevalent type of metallo-β-lactamase and hydrolyzes almost all clinically used β-lactam antibiotics, including last line antibiotic - carbapenems. Inactivating NDM-1 and then reviving carbapenems holds great promise in treating NDM-1-producing bacteria, while better intracellular antibiotic accumulation and precise NDM-1 inactivation are challenges. Herein, a photothermal-assisted biomimetic antibiotic booster (PBM) is engineered for repurposing carbapenems against NDM-1-producing bacteria by photothermal-induced bacterial membrane destabilizing and synchronous NDM-1 inactivation. In the PBM nanomedicine, stomach medicine-converted bismuth nanoclusters (BiNCs) with excellent photothermal effect are used as photothermal skeleton, loading meropenem (MEM) on the inside and coating platelet membrane vesicles (PMVs) on the outside. Due to the inherent inflammatory properties of platelets, PBM exhibits an excellent homing effect on infectious sites and precise homologous targeting of pathogens. Meanwhile, due to the excellent photothermal properties of bismuth nanoclusters, PBM enhanced the intracellular accumulation of meropenem via breaking the bacterial outer membrane barrier, and then effectively inactivated NDM-1. Benefiting from the combination of photothermal-assisted bacterial membrane destabilizing and NDM-1 inactivation, PBM (100 μg/mL) effectively reversed the resistance of NDM-1-producing Escherichia coli to meropenem, exhibiting a more significant antibacterial effect (sterilization rate reached 98.9 %) than equivalent meropenem alone by time-killing curve.

{"title":"Pathogen-targeting bismuth nanocluster via photothermally-mediated NDM-1 inactivation and bacterial membrane destabilization combat NDM-1-producing bacteria","authors":"Xichan He , Shaobing Liu , Jianan Tong , Yuhui Qiao , Xue Li , Kaixuan Dong , Dongsheng Yu , Yanshu Gu","doi":"10.1016/j.procbio.2024.12.011","DOIUrl":"10.1016/j.procbio.2024.12.011","url":null,"abstract":"<div><div>New Delhi metallo-β-lactamase-1 (NDM-1) is the most prevalent type of metallo-β-lactamase and hydrolyzes almost all clinically used β-lactam antibiotics, including last line antibiotic - carbapenems. Inactivating NDM-1 and then reviving carbapenems holds great promise in treating NDM-1-producing bacteria, while better intracellular antibiotic accumulation and precise NDM-1 inactivation are challenges. Herein, a photothermal-assisted biomimetic antibiotic booster (PBM) is engineered for repurposing carbapenems against NDM-1-producing bacteria by photothermal-induced bacterial membrane destabilizing and synchronous NDM-1 inactivation. In the PBM nanomedicine, stomach medicine-converted bismuth nanoclusters (BiNCs) with excellent photothermal effect are used as photothermal skeleton, loading meropenem (MEM) on the inside and coating platelet membrane vesicles (PMVs) on the outside. Due to the inherent inflammatory properties of platelets, PBM exhibits an excellent homing effect on infectious sites and precise homologous targeting of pathogens. Meanwhile, due to the excellent photothermal properties of bismuth nanoclusters, PBM enhanced the intracellular accumulation of meropenem via breaking the bacterial outer membrane barrier, and then effectively inactivated NDM-1. Benefiting from the combination of photothermal-assisted bacterial membrane destabilizing and NDM-1 inactivation, PBM (100 μg/mL) effectively reversed the resistance of NDM-1-producing <em>Escherichia coli</em> to meropenem, exhibiting a more significant antibacterial effect (sterilization rate reached 98.9 %) than equivalent meropenem alone by time-killing curve.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 158-168"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of novel D-lyxose isomerases from the goat rumen metagenome

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.014

Xin Xu , Mingyi Chen , Yali Su , Jiayun Ding , Xiuye Xie , Xinyi Zhang , Feng Li , Xian’ai Shi , Guozeng Wang

Biocatalytic technology has emerged as a powerful tool for synthesizing functional sugars with a wide range of physiological functions. D-lyxose isomerase is an important aldose–ketose isomerase used for synthesizing functional sugars such as L-ribose and D-mannose; however, at present, only a few D-lyxose isomerases have been studied. In this study, a metagenomic approach was employed to mine novel D-lyxose isomerases from the goat rumen microbiome. Eleven full-length D-lyxose isomerase genes were identified using sequence alignment and phylogenetic analysis. All 11 genes were classified as Group Ⅱ D-lyxose isomerases and display low similarity to previously characterized D-lyxose isomerases. Five of the genes were selected for heterologous expression in Escherichia coli, and all were expressed successfully and had detectable enzyme activity. Further characterization revealed that optimum temperatures for the five enzymes range from 45 °C to 60 °C, and the optimum pH range is 7.0–8.0, with high relative residual activities under weakly acidic conditions. The recombinant enzymes have a broad substrate spectrum and are active against L-ribose, L-ribulose, D-fructose, and D-mannose, in addition to exhibiting the highest activity against D-lyxose. Among the five enzymes, GR-LI2 displays the highest activity toward L-ribulose, and GR-LI5 displays the highest activity against fructose, suggesting that these two enzymes have significant potential for L-ribose and D-mannose synthesis, respectively.

{"title":"Identification and characterization of novel D-lyxose isomerases from the goat rumen metagenome","authors":"Xin Xu , Mingyi Chen , Yali Su , Jiayun Ding , Xiuye Xie , Xinyi Zhang , Feng Li , Xian’ai Shi , Guozeng Wang","doi":"10.1016/j.procbio.2024.12.014","DOIUrl":"10.1016/j.procbio.2024.12.014","url":null,"abstract":"<div><div>Biocatalytic technology has emerged as a powerful tool for synthesizing functional sugars with a wide range of physiological functions. D-lyxose isomerase is an important aldose–ketose isomerase used for synthesizing functional sugars such as L-ribose and D-mannose; however, at present, only a few D-lyxose isomerases have been studied. In this study, a metagenomic approach was employed to mine novel D-lyxose isomerases from the goat rumen microbiome. Eleven full-length D-lyxose isomerase genes were identified using sequence alignment and phylogenetic analysis. All 11 genes were classified as Group Ⅱ D-lyxose isomerases and display low similarity to previously characterized D-lyxose isomerases. Five of the genes were selected for heterologous expression in <em>Escherichia coli</em>, and all were expressed successfully and had detectable enzyme activity. Further characterization revealed that optimum temperatures for the five enzymes range from 45 °C to 60 °C, and the optimum pH range is 7.0–8.0, with high relative residual activities under weakly acidic conditions. The recombinant enzymes have a broad substrate spectrum and are active against L-ribose, L-ribulose, D-fructose, and D-mannose, in addition to exhibiting the highest activity against D-lyxose. Among the five enzymes, GR-LI2 displays the highest activity toward L-ribulose, and GR-LI5 displays the highest activity against fructose, suggesting that these two enzymes have significant potential for L-ribose and D-mannose synthesis, respectively.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 213-221"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review on integration of cellular metabolic engineering and cell-free systems for microbial platforms

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.12.010

Arunangshu Das , Anita Verma , Naba Hazarika

In response to the demand for environment friendly synthesis of chemical feedstocks, two disciplines have emerged: cellular metabolic engineering (CME) and cell-free metabolic engineering (CFME). Cell free systems largely replicate cellular pathways in vitro to bypass the need of live cells to achieve greater control and process simplicity. However, not all cellular biochemical aspects can be replicated in vitro, as some are not hardwired but crucial modulators of biochemical processes. These include metabolic states defined by ensemble of small molecules that influence proteostasis, catalytic activity of enzymes, and redox power, influencing cellular anabolic and catabolic decisions. Despite the advancement of molecular biology techniques engineering such control systems remains largely a challenge for cellular metabolic engineering. This review thoroughly examines these limitations in both fields and explores the potential for implementing non-hardwired control systems in cell-free metabolic engineering, either independently or in combination with cellular metabolic engineering. Further, the integration of chemistry and machine learning models is considered, with a focus on how their combined strengths can be leveraged to develop novel synthetic schemes.

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引用次数: 0

The start-up of red blood cell-like Anammox granular sludge based on substrate volume flux

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.procbio.2024.11.035

Wenqiang Wang , Li Wang , Qu Wang , Yang Su , Jun Li , Dong Li

Enhancing substrate transfer is crucial for promoting the nitrogen removal rate of Anammox granular sludge, as the shape of the granules significantly influences substrate diffusion. In this study, inspired by biomimicry principles, we propose a novel red blood cell-like granular sludge to increase the contact area and improve substrate transfer. The startup and operation of Anammox granular sludge in low-strength ammonia nitrogen wastewater were successfully achieved through an increase in substrate volume flux (SVF). As the SVF increased from 2.38 to 7.43 L²/(g VSS·h), specific Anammox activity (SAA) rose from 0.138 to 0.483 g N/(g VSS·d). The Anammox granular sludge system achieved a total nitrogen removal efficiency of 85.70 % and a nitrogen removal rate (NRR) of 0.792 kg N/(m³·d) after 126 days. Notably, red blood cell-like granular sludge was observed by day 116. The rise in SVF induced a granule structure more conducive to substrate transport, improving the granules' efficiency in substrate uptake and thereby enhancing their SAA. High-throughput pyrosequencing revealed that the dominant bacteria using the SVF-enhancing strategy remained anaerobic ammonium oxidizing bacteria (AnAOB) (29.53 %-29.89 %), with an increased diversity of species, including Candidatus Kuenenia, Candidatus Anammoxoglobus, and Candidatus Brocadia.

{"title":"The start-up of red blood cell-like Anammox granular sludge based on substrate volume flux","authors":"Wenqiang Wang , Li Wang , Qu Wang , Yang Su , Jun Li , Dong Li","doi":"10.1016/j.procbio.2024.11.035","DOIUrl":"10.1016/j.procbio.2024.11.035","url":null,"abstract":"<div><div>Enhancing substrate transfer is crucial for promoting the nitrogen removal rate of Anammox granular sludge, as the shape of the granules significantly influences substrate diffusion. In this study, inspired by biomimicry principles, we propose a novel red blood cell-like granular sludge to increase the contact area and improve substrate transfer. The startup and operation of Anammox granular sludge in low-strength ammonia nitrogen wastewater were successfully achieved through an increase in substrate volume flux (SVF). As the SVF increased from 2.38 to 7.43 L²/(g VSS·h), specific Anammox activity (SAA) rose from 0.138 to 0.483 g N/(g VSS·d). The Anammox granular sludge system achieved a total nitrogen removal efficiency of 85.70 % and a nitrogen removal rate (NRR) of 0.792 kg N/(m³·d) after 126 days. Notably, red blood cell-like granular sludge was observed by day 116. The rise in SVF induced a granule structure more conducive to substrate transport, improving the granules' efficiency in substrate uptake and thereby enhancing their SAA. High-throughput pyrosequencing revealed that the dominant bacteria using the SVF-enhancing strategy remained anaerobic ammonium oxidizing bacteria (AnAOB) (29.53 %-29.89 %), with an increased diversity of species, including <em>Candidatus Kuenenia</em>, <em>Candidatus Anammoxoglobus</em>, and <em>Candidatus Brocadia</em>.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 121-127"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-assisted deep eutectic solvent extraction of flavonoids from Hippophae plants: Optimization and α-glucosidase inhibition studies

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-01-31 DOI: 10.1016/j.procbio.2025.01.027

Yuyuan Huang , Hong Yang , Ying Zhang , Tongtong Zhang , Xiaoqiang Chen

To make full use of Hippophae plant resources, we use the green extraction method of deep eutectic solvent (DES) combined with ultrasonication to extract flavonoids. HPLC was used to measure four flavonoids (isorhamnetin-3-O-glucoside-7-O-rhamnoside, rutin, isorhamnetin-3-O-rutinoside, and isorhamnetin) in fruit samples and five flavonoids (quercetin-7-O-glucoside, isorhamnetin-3-O-glucoside-7-O-rhamnoside, rutin, guaijaverin, and kaempferol-3-O-rutinoside) in leaf samples. The best solvent was chosen from 12 kinds of DES. With betaine/acetic acid (molar ratio of 1:2) as the extraction solvent, a liquid-solid ratio of 21:1 (mL/g), an extraction temperature of 37 °C, and an extraction time of 22 min, the total content of four flavonoids in the fruit was 10.547 mg/g. With L-proline/levulinic acid (molar ratio of 1:2) as the extraction solvent, a liquid-solid ratio of 31:1 (mL/g), an extraction temperature of 49 °C, and an extraction time of 22 min, the yield of five flavonoids in the leaves was 17.921 mg/g. The extraction efficiency of this method was significantly better than that of hot reflux extraction and 60 % ethanol extraction (p < 0.05). The flavonoids obtained by further purification of the extracts from the fruit and leaves exhibited inhibitory ability to α-glucosidase in vitro, IC₅₀ = 28.189 and 19.675 μg/mL, respectively. Above all, the use of optimized DES could efficiently extract flavonoids from Hippophae plants, which have promising applications in the regulation of blood glucose.

{"title":"Ultrasound-assisted deep eutectic solvent extraction of flavonoids from Hippophae plants: Optimization and α-glucosidase inhibition studies","authors":"Yuyuan Huang , Hong Yang , Ying Zhang , Tongtong Zhang , Xiaoqiang Chen","doi":"10.1016/j.procbio.2025.01.027","DOIUrl":"10.1016/j.procbio.2025.01.027","url":null,"abstract":"<div><div>To make full use of <em>Hippophae</em> plant resources, we use the green extraction method of deep eutectic solvent (DES) combined with ultrasonication to extract flavonoids. HPLC was used to measure four flavonoids (isorhamnetin-3-<em>O</em>-glucoside-7-<em>O</em>-rhamnoside, rutin, isorhamnetin-3-<em>O</em>-rutinoside, and isorhamnetin) in fruit samples and five flavonoids (quercetin-7-<em>O</em>-glucoside, isorhamnetin-3-<em>O</em>-glucoside-7-<em>O</em>-rhamnoside, rutin, guaijaverin, and kaempferol-3-<em>O</em>-rutinoside) in leaf samples. The best solvent was chosen from 12 kinds of DES. With betaine/acetic acid (molar ratio of 1:2) as the extraction solvent, a liquid-solid ratio of 21:1 (mL/g), an extraction temperature of 37 °C, and an extraction time of 22 min, the total content of four flavonoids in the fruit was 10.547 mg/g. With L-proline/levulinic acid (molar ratio of 1:2) as the extraction solvent, a liquid-solid ratio of 31:1 (mL/g), an extraction temperature of 49 °C, and an extraction time of 22 min, the yield of five flavonoids in the leaves was 17.921 mg/g. The extraction efficiency of this method was significantly better than that of hot reflux extraction and 60 % ethanol extraction (<em>p</em> < 0.05). The flavonoids obtained by further purification of the extracts from the fruit and leaves exhibited inhibitory ability to α-glucosidase in <em>vitro</em>, IC<sub>50</sub> = 28.189 and 19.675 μg/mL, respectively. Above all, the use of optimized DES could efficiently extract flavonoids from <em>Hippophae</em> plants, which have promising applications in the regulation of blood glucose.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"151 ","pages":"Pages 43-51"},"PeriodicalIF":3.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143271148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in enzyme-based metal nanoparticles as colorimetric biosensors for the detection of pesticides: A review

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-01-30 DOI: 10.1016/j.procbio.2025.01.028

Nor Khairun Bariah Kamaruddin , Shahrul Ainliah Alang Ahmad , Anis Abdul Ghani

Pesticides residues determination has received significant interest due to the high acute toxicity and their potential to cause long-term damage to the environment and human lives. Traditional analytical methods such as HPLC and GC-MS have been widely employed to analyse pesticides in various samples. However, these methods have drawbacks such as tedious sample preparation, complexity, expensive, and the need for highly specialized staff. As a result, there is an increasing demand for analytical methods that enable easy, quick, sensitive, selective, low-cost, and reliable detection of pesticides at trace levels. Enzyme-based biosensors offer a promising alternative, providing rapid, sensitive, and cost-effective pesticide detection. Recent advancements in colorimetric biosensors using metal nanoparticles enable on-site monitoring visible by the naked eye. These innovations may lead to portable instruments for rapid toxicity testing, revolutionizing pesticide analysis. This paper reviews insecticide types, effects, challenges, and the development of metal nanoparticle-based colorimetric biosensors for identifying pesticide.

{"title":"Recent advances in enzyme-based metal nanoparticles as colorimetric biosensors for the detection of pesticides: A review","authors":"Nor Khairun Bariah Kamaruddin , Shahrul Ainliah Alang Ahmad , Anis Abdul Ghani","doi":"10.1016/j.procbio.2025.01.028","DOIUrl":"10.1016/j.procbio.2025.01.028","url":null,"abstract":"<div><div>Pesticides residues determination has received significant interest due to the high acute toxicity and their potential to cause long-term damage to the environment and human lives. Traditional analytical methods such as HPLC and GC-MS have been widely employed to analyse pesticides in various samples. However, these methods have drawbacks such as tedious sample preparation, complexity, expensive, and the need for highly specialized staff. As a result, there is an increasing demand for analytical methods that enable easy, quick, sensitive, selective, low-cost, and reliable detection of pesticides at trace levels. Enzyme-based biosensors offer a promising alternative, providing rapid, sensitive, and cost-effective pesticide detection. Recent advancements in colorimetric biosensors using metal nanoparticles enable on-site monitoring visible by the naked eye. These innovations may lead to portable instruments for rapid toxicity testing, revolutionizing pesticide analysis. This paper reviews insecticide types, effects, challenges, and the development of metal nanoparticle-based colorimetric biosensors for identifying pesticide.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"151 ","pages":"Pages 99-112"},"PeriodicalIF":3.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reaction pathways and integral kinetics for hydrolysis of soybean oligosaccharides by α-galactosidase and invertase

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-01-30 DOI: 10.1016/j.procbio.2025.01.021

Md Fauzul Kabir , Calum Bochenek , Chrys Wesdemiotis , Lu-Kwang Ju

The increasingly produced soybean molasses lacks value-added applications because its oligosaccharides stachyose and raffinose are poorly digestible/hydrolysable by many organisms. Enzymatic upgrading can be effective, but no study has previously examined and modeled its full complexity. This study addresses this knowledge gap by developing comprehensive models and evaluating them with results of enzymatic hydrolysis of, progressively, pure sucrose, melibiose, raffinose and stachyose, and then soybean molasses. The models considered all possible reaction pathways, competitive inhibition among substrates and intermediates, and kinetic decline of enzyme activities. The kinetic parameters, obtained from regression of concentration profiles for all carbohydrates (substrate, intermediates, and products), offered important insights into enzymatic hydrolysis of soybean galacto-oligosaccharides. Most importantly, digalactose was, for the first time, shown to form appreciably during stachyose hydrolysis (by breakage of mid-structure α-1,6 galactose-glucose bond), with rates comparable to the commonly recognized raffinose formation (by cleavage of terminal galactose). On the other hand, the invertase-catalyzed breakdown of stachyose to manninotriose (+ fructose) was much less significant. Further, the A. niger invertase could remove fructose from sucrose, raffinose, and stachyose, but with substantially different effectiveness: sucrose > raffinose > stachyose. Practically, the models developed are valuable to the development of enzymatic soybean-molasses upgrading process.

{"title":"Reaction pathways and integral kinetics for hydrolysis of soybean oligosaccharides by α-galactosidase and invertase","authors":"Md Fauzul Kabir , Calum Bochenek , Chrys Wesdemiotis , Lu-Kwang Ju","doi":"10.1016/j.procbio.2025.01.021","DOIUrl":"10.1016/j.procbio.2025.01.021","url":null,"abstract":"<div><div>The increasingly produced soybean molasses lacks value-added applications because its oligosaccharides stachyose and raffinose are poorly digestible/hydrolysable by many organisms. Enzymatic upgrading can be effective, but no study has previously examined and modeled its full complexity. This study addresses this knowledge gap by developing comprehensive models and evaluating them with results of enzymatic hydrolysis of, progressively, pure sucrose, melibiose, raffinose and stachyose, and then soybean molasses. The models considered all possible reaction pathways, competitive inhibition among substrates and intermediates, and kinetic decline of enzyme activities. The kinetic parameters, obtained from regression of concentration profiles for all carbohydrates (substrate, intermediates, and products), offered important insights into enzymatic hydrolysis of soybean galacto-oligosaccharides. Most importantly, digalactose was, for the first time, shown to form appreciably during stachyose hydrolysis (by breakage of mid-structure α-1,6 galactose-glucose bond), with rates comparable to the commonly recognized raffinose formation (by cleavage of terminal galactose). On the other hand, the invertase-catalyzed breakdown of stachyose to manninotriose (+ fructose) was much less significant. Further, the <em>A. niger</em> invertase could remove fructose from sucrose, raffinose, and stachyose, but with substantially different effectiveness: sucrose > raffinose > stachyose. Practically, the models developed are valuable to the development of enzymatic soybean-molasses upgrading process.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 357-370"},"PeriodicalIF":3.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel extraction and purification technologies for anthraquinones: Towards sustainable food preservation solutions

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-01-27 DOI: 10.1016/j.procbio.2025.01.020

Juanjuan Yang , Zerong Pei , Hongyi Yang , Wenwen Deng , Hui Li

Food preservation technologies are pivotal for extending the shelf life of food products by incorporating chemical preservatives, thus ensuring the maintenance of flavor, nutritional content, and safety. As consumer demand for food safety and quality continues to escalate, there has been a burgeoning interest in natural alternatives due to their gentle antimicrobial properties and enhanced safety profiles. Among these, anthraquinones, predominantly derived from botanical sources such as rhubarb, senna, aloe vera, knotweed, and madder, have garnered significant attention. Recent findings have underscored the exceptional antimicrobial and antioxidant properties of anthraquinones, positioning them as promising candidates for natural food preservatives with substantial developmental and application prospects within the food preservation sector. Therefore, there has been a surge in the development of innovative extraction and purification methodologies aimed at harnessing natural anthraquinones from their botanical reservoirs. This review summarizes the current state of research on extraction and purification techniques for anthraquinones from a variety of natural plant species, along with an evaluation of their antimicrobial and antioxidant efficacies in the context of food preservation. Additionally, it delves into the preservative properties of anthraquinone compounds to offer insights that may facilitate efficient production and utilization of natural anthraquinones for food preservation.

{"title":"Novel extraction and purification technologies for anthraquinones: Towards sustainable food preservation solutions","authors":"Juanjuan Yang , Zerong Pei , Hongyi Yang , Wenwen Deng , Hui Li","doi":"10.1016/j.procbio.2025.01.020","DOIUrl":"10.1016/j.procbio.2025.01.020","url":null,"abstract":"<div><div>Food preservation technologies are pivotal for extending the shelf life of food products by incorporating chemical preservatives, thus ensuring the maintenance of flavor, nutritional content, and safety. As consumer demand for food safety and quality continues to escalate, there has been a burgeoning interest in natural alternatives due to their gentle antimicrobial properties and enhanced safety profiles. Among these, anthraquinones, predominantly derived from botanical sources such as rhubarb, senna, aloe vera, knotweed, and madder, have garnered significant attention. Recent findings have underscored the exceptional antimicrobial and antioxidant properties of anthraquinones, positioning them as promising candidates for natural food preservatives with substantial developmental and application prospects within the food preservation sector. Therefore, there has been a surge in the development of innovative extraction and purification methodologies aimed at harnessing natural anthraquinones from their botanical reservoirs. This review summarizes the current state of research on extraction and purification techniques for anthraquinones from a variety of natural plant species, along with an evaluation of their antimicrobial and antioxidant efficacies in the context of food preservation. Additionally, it delves into the preservative properties of anthraquinone compounds to offer insights that may facilitate efficient production and utilization of natural anthraquinones for food preservation.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 328-341"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction, purification, component analysis and bioactivity of polyphenols from wampee

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-01-27 DOI: 10.1016/j.procbio.2025.01.025

Yuan-yuan Ren, Mosa Makhele, Jue-jun Zhou, Peng-peng Sun

Wampee, a kind of medicinal and edible fruit, contains abundant bioactive compounds. In order to realize comprehensive utilization of wampee by-products, the extraction parameters for wampee polyphenols were optimized using single-factor and response surface tests, followed by the purification with AB-8 macroporous resin. Then component analysis and bioactivity evaluation in vitro were conducted. Result indicated the optimal extraction parameters of wampee polyphenols were: ethanol concentration 56 %, extraction temperature 62℃, material-liquid ratio being 1:19 g/mL, and extraction time 1 h. AB-8 resin exhibited high adsorption rate (91.42 % ± 0.18 %) and desorption rate (89.60 % ± 0.90 %), leading to increased purity of wampee polyphenols (WPP) from 12.51 % ± 0.36–53.8 % ± 1.87 %. UV and FTIR spectra confirmed the presence of polyphenolic compounds in WPP, including unique phenolic Vc-O and primary alcohol structures. LC-MS analysis identified six distinct polyphenolic compounds in WPP. In addition, the bioactivity of WPP enhanced, especially hydroxyl radical scavenging ability. Our research would provide guidance for the comprehensive utilization of wampee resources and its application in functional foods.

{"title":"Extraction, purification, component analysis and bioactivity of polyphenols from wampee","authors":"Yuan-yuan Ren, Mosa Makhele, Jue-jun Zhou, Peng-peng Sun","doi":"10.1016/j.procbio.2025.01.025","DOIUrl":"10.1016/j.procbio.2025.01.025","url":null,"abstract":"<div><div>Wampee, a kind of medicinal and edible fruit, contains abundant bioactive compounds. In order to realize comprehensive utilization of wampee by-products, the extraction parameters for wampee polyphenols were optimized using single-factor and response surface tests, followed by the purification with AB-8 macroporous resin. Then component analysis and bioactivity evaluation in vitro were conducted. Result indicated the optimal extraction parameters of wampee polyphenols were: ethanol concentration 56 %, extraction temperature 62℃, material-liquid ratio being 1:19 g/mL, and extraction time 1 h. AB-8 resin exhibited high adsorption rate (91.42 % ± 0.18 %) and desorption rate (89.60 % ± 0.90 %), leading to increased purity of wampee polyphenols (WPP) from 12.51 % ± 0.36–53.8 % ± 1.87 %. UV and FTIR spectra confirmed the presence of polyphenolic compounds in WPP, including unique phenolic Vc-O and primary alcohol structures. LC-MS analysis identified six distinct polyphenolic compounds in WPP. In addition, the bioactivity of WPP enhanced, especially hydroxyl radical scavenging ability. Our research would provide guidance for the comprehensive utilization of wampee resources and its application in functional foods.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 318-327"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming dairy effluent into valuable resources: Harnessing microalgae for sustainable production of nutraceuticals and pharmaceuticals

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-01-27 DOI: 10.1016/j.procbio.2025.01.026

Udaypal, Rahul Kumar Goswami, Pradeep Verma

The dairy industry generates nutrient-rich effluent, significantly contributing to global eutrophication and waterborne diseases. Dairy effluent (DE) can be transformed from waste into valuable products by using it as a cultivation medium for microalgae. Microalgae have high biomass productivity and are rich in lipids, proteins, carbohydrates, vitamins, and carotenoids. These compounds are in high demand in the nutraceutical and pharmaceutical industries. This review examines DE as a sustainable alternative to synthetic media for cultivating microalgae, which is rich in nutrients like nitrogen, phosphorus, and lactose. It compares the composition of processed dairy effluent (PDE) with synthetic media, showcasing its potential for microalgae cultivation for nutraceutical and pharmaceutical applications. The review also highlights the environmental risks of untreated DE and advocates for PDE-based cultivation as an eco-friendly solution. It discusses photobioreactor (PBR) designs optimized for PDE treatment and explores the global market for microalgal products. Challenges such as scaling production and regulatory hurdles have been discussed, emphasizing the need for sustainable microalgae cultivation. This review advances algal biorefinery research by presenting DE as a sustainable cultivation medium for producing high-value microalgal products using suggested PBRs. It highlights strategies to integrate wastewater treatment with bioproduct generation, fostering scalable and eco-friendly solutions.

{"title":"Transforming dairy effluent into valuable resources: Harnessing microalgae for sustainable production of nutraceuticals and pharmaceuticals","authors":"Udaypal, Rahul Kumar Goswami, Pradeep Verma","doi":"10.1016/j.procbio.2025.01.026","DOIUrl":"10.1016/j.procbio.2025.01.026","url":null,"abstract":"<div><div>The dairy industry generates nutrient-rich effluent, significantly contributing to global eutrophication and waterborne diseases. Dairy effluent (DE) can be transformed from waste into valuable products by using it as a cultivation medium for microalgae. Microalgae have high biomass productivity and are rich in lipids, proteins, carbohydrates, vitamins, and carotenoids. These compounds are in high demand in the nutraceutical and pharmaceutical industries. This review examines DE as a sustainable alternative to synthetic media for cultivating microalgae, which is rich in nutrients like nitrogen, phosphorus, and lactose. It compares the composition of processed dairy effluent (PDE) with synthetic media, showcasing its potential for microalgae cultivation for nutraceutical and pharmaceutical applications. The review also highlights the environmental risks of untreated DE and advocates for PDE-based cultivation as an eco-friendly solution. It discusses photobioreactor (PBR) designs optimized for PDE treatment and explores the global market for microalgal products. Challenges such as scaling production and regulatory hurdles have been discussed, emphasizing the need for sustainable microalgae cultivation. This review advances algal biorefinery research by presenting DE as a sustainable cultivation medium for producing high-value microalgal products using suggested PBRs. It highlights strategies to integrate wastewater treatment with bioproduct generation, fostering scalable and eco-friendly solutions.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"150 ","pages":"Pages 342-356"},"PeriodicalIF":3.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0