Process Biochemistry最新文献_第2页

Extraction of wool polypeptides using L-cysteine and protease: a study of the successive hydrolysis of the fibres’ regions 用l -半胱氨酸和蛋白酶提取羊毛多肽：纤维区域连续水解的研究

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-14 DOI: 10.1016/j.procbio.2026.01.011

Clémence Marie-Renée Belbéoch, Jinsong Shen, Edward Smith

Wool is a natural fibre with a diversity of applications in the textile industry. Its nature as a protein-rich fibre constituted of several distinct but interrelated regions, confers its unique mechanical properties. Due to the competition with superfine wools and the emergence of synthetic alternatives, coarser wools from the Northern hemisphere have suffered from a lack of valorising paths. Recently, studies successfully extracted wool polypeptides using sulphur-based reducing agents, such as sodium sulphite. These methods can separate fibres from textile blends for recycling purposes. In addition, the different wool protein types could be isolated to explore their post-consumer valorisation into new applications. In this study, the effectiveness of the amino acid, L-cysteine, as a sustainable reducing agent, combined with the activity of a protease on wool hydrolysis and proteins’ extraction was assessed, under different concentrations of L-cysteine and enzyme and duration of the reaction. Understanding the chronological order of the wool regions’ lysis during the extraction process is crucial for the control of specific parameters to selectively recover microfibrils or matrix proteins – which exhibit different protein structures. This selective recovery of the different wool proteins could support their regeneration into innovative materials, for applications in composites, packaging or construction.

羊毛是一种天然纤维，在纺织工业中有多种用途。它是一种富含蛋白质的纤维，由几个不同但相互关联的区域组成，这赋予了它独特的机械性能。由于与超细羊毛的竞争以及合成替代品的出现，来自北半球的粗羊毛一直缺乏增值途径。近年来，研究成功地利用硫基还原剂，如亚硫酸钠提取羊毛多肽。这些方法可以将纤维从纺织混纺物中分离出来，以便回收利用。此外，不同的羊毛蛋白类型可以被分离出来，以探索其消费后的价值转化为新的应用。在本研究中，考察了氨基酸l -半胱氨酸作为一种可持续还原剂，结合蛋白酶的活性，在不同浓度的l -半胱氨酸、酶和反应时间下对羊毛水解和蛋白质提取的有效性。在提取过程中，了解羊毛区域裂解的时间顺序对于控制特定参数以选择性地回收微原纤维或基质蛋白至关重要，因为微原纤维或基质蛋白具有不同的蛋白质结构。这种对不同羊毛蛋白的选择性回收可以支持它们再生成创新材料，用于复合材料、包装或建筑。

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引用次数: 0

An integrated hydrothermal-enzymatic biorefinery approach for efficient ferulic acid production from Euryale ferox seed shell via lignin removal 木质素去除法从芡实种子壳中高效生产阿魏酸的一体化水热-酶生物精炼方法

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-14 DOI: 10.1016/j.procbio.2026.01.013

Shiquan Qian , Xinran Cheng , Wenxing Song , Youyou Zheng , Ruojun Tang , Jingjing Fan , Ci Jin , Enjie Diao

The effectiveness of combined hydrothermal and enzymic hydrolysis for ferulic acid recovery was investigated. Combined hydrothermal, cellulase, xylanase and ferulic acid esterase hydrolysis (HCXF) proved to be resulted in disruption of lignocellulose fibers and removal of hemicellulose and lignin in pretreated Euryale ferox seed shell, thus improved enzymatic saccharification. Interestingly, when performed using HCXF via hydrothermal hydrolysis (29.7 ml/g, 110 °C, 1.0 h) followed by enzymatic hydrolysis with 1:2:2 (v/v) of cellulase (31.3 ml/g, 1.0 %): xylanase (34.5 ml/g, 0.7 %): ferulic acid esterase (16.6 ml/g, 0.7 %) for 36 h, 2.21 mg/g of ferulic acid recovery and 30.82 % of lignin removal rate were obtained. Moreover, the ferulic acid extracts obtained by HCXF exhibited strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals scavenging effects, showing the IC₅₀ value at 0.19 and 0.11 mg/ml, respectively. HCXF process efficiently improved the recovery of ferulic acid through lignin removal in pretreated E. ferox seed shell, leading to having the potential application in food or pharmaceutical industry as antioxidant ingredients.

研究了水热法和酶解法联合回收阿魏酸的效果。水热、纤维素酶、木聚糖酶和阿威酸酯酶联合水解（HCXF）可使预处理后的芡实籽壳中的木质纤维素纤维断裂，半纤维素和木质素脱除，从而改善酶促糖化过程。有趣的是,当使用HCXF执行通过水热水解(29.7 ml / g、110°C, 1.0 h)其次是酶法水解1:2:2 (v / v)的纤维素酶(31.3 ml / g、1.0 %):木聚糖酶(34.5 ml / g、0.7 %):阿魏酸酯酶(16.6 ml / g、0.7 %)36 h, 2.21毫克/克阿魏酸复苏和30.82 %的木质素去除率。此外，HCXF制备的阿魏酸提取物具有较强的DPPH和羟基自由基清除作用，IC50值分别为0.19和0.11 mg/ml。HCXF工艺通过去除预处理铁籽壳中的木质素，有效地提高了阿魏酸的回收率，作为抗氧化成分在食品或制药工业中具有潜在的应用前景。

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引用次数: 0

Degradation and control strategies for microplastics during aerobic composting: A review 微塑料在好氧堆肥过程中的降解及控制策略综述

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-13 DOI: 10.1016/j.procbio.2026.01.009

Ping Liu, Hongli Huang, Xifeng Qin, Yuewei He, Jiahua Liu, Jiachao Zhang, Yuan Yang, Lihua Zhang

Microplastics (MPs) pose a significant threat to the soil environment, food security, and human health. The application of organic fertilizers is recognized as a primary pathway for the introduction of MPs into agricultural soils. Research on the degradation of MPs during aerobic composting is in an early stage, and the degradation and control strategies for MPs during aerobic composting remain insufficiently researched. This paper systematically summarizes the sources, abundance, and occurrence characteristics of MPs in compost raw materials, including municipal sludge, livestock manure, kitchen waste, and household waste. It concludes the changes of MPs during composting and their degradation mechanisms: physical, chemical, and biological mechanisms. Furthermore, the paper summarizes the methods for enhancing the degradation of MPs, such as ultra-high-temperature composting, exogenous additives, and pretreatment technologies. Finally, the research directions are proposed in aspects of the detection methods of MPs, degradation mechanisms, enhancement of degradation approaches, environmental behavior of degradation products, and the risks of combined pollution by MPs. This paper aimed to provide insights for the control and management of MPs pollution.

微塑料对土壤环境、粮食安全和人类健康构成重大威胁。有机肥的施用被认为是MPs进入农业土壤的主要途径。对好氧堆肥过程中多聚物降解的研究尚处于起步阶段，对好氧堆肥过程中多聚物的降解及控制策略研究较少。本文系统综述了城市污泥、畜禽粪便、厨余垃圾、生活垃圾等堆肥原料中MPs的来源、丰度和赋存特征。总结了堆肥过程中MPs的变化及其降解机制：物理机制、化学机制和生物机制。此外，本文还综述了提高MPs降解的方法，如超高温堆肥、外源添加剂和预处理技术。最后，从MPs的检测方法、降解机理、降解途径的改进、降解产物的环境行为、MPs复合污染风险等方面提出了今后的研究方向。本文旨在为MPs污染的控制和管理提供参考。

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引用次数: 0

Microaerobic bio-pretreatment of straw with fermentation broth as inoculum: Enhancing hydrolysis-acidification efficiency 以发酵液为接种物的秸秆微氧预处理：提高水解酸化效率

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-13 DOI: 10.1016/j.procbio.2026.01.005

Chuqiao Wang , Xiaoqian Liu , Jialong Pan , Shan Huang , Xizi Long , Yuying Hu , Jiajie Zhang

Anaerobic digestion (AD) has been recognized as a promising technology for the disposal of agricultural residues, such as straw. Nonetheless, AD encounters a bottleneck of low hydrolysis rate. Microaerobic pretreatment significantly enhances straw AD efficiency, but its effectiveness depends on factors like pretreatment time and water content, with their interactions remaining unclear. Therefore, this study employed a response surface methodology (RSM) to investigate the interactions among pretreatment time, water content, and volatile fatty acids (VFAs) concentration in inoculum during the microaerobic pretreatment. The results showed that the highest hydrolysis-acidification efficiency was achieved with a pretreatment time of 10 days and a VFAs concentration of 10,000 mg/L, and there was a notable interaction between these two factors in the inoculum. Additionally, microbial community analysis showed that microaerobic pretreatment altered the microbial community structure to adapt to micro-oxygen environments, promoting the hydrolysis-acidification of straw. Specifically, Proteobacteria, Firmicutes, and Actinobacteria became the predominant acid-producing bacteria following microaerobic pretreatment. Within the fungus communities, Aspergillus niger and Penicillium citrinum, known for lignin degradation, increased by 4.4-fold and 1.2-fold, respectively. And the Methanobacteria became the main group in the archaeal communities. This paper presents a new perspective on the pretreatment of lignocellulose in commercial anaerobic reactors.

厌氧消化（AD）已被认为是一种很有前途的农业废弃物处理技术，如秸秆。然而，AD遇到了低水解率的瓶颈。微氧预处理可显著提高秸秆AD效率，但其效果受预处理时间和含水量等因素的影响，其相互作用尚不清楚。因此，本研究采用响应面法（RSM）研究了预处理时间、接种物含水量和挥发性脂肪酸（VFAs）浓度在微氧预处理过程中的相互作用。结果表明，预处理时间为10 d， VFAs浓度为10000 mg/L时水解酸化效率最高，且接种物中这两个因素之间存在显著的交互作用。此外，微生物群落分析表明，微氧预处理改变了微生物群落结构以适应微氧环境，促进了秸秆的水解酸化。具体来说，变形菌门、厚壁菌门和放线菌门在微氧预处理后成为主要的产酸细菌。在真菌群落中，以木质素降解著称的黑曲霉（Aspergillus niger）和柑橘青霉（Penicillium citriinum）分别增加了4.4倍和1.2倍。甲烷杆菌成为古细菌群落的主要类群。介绍了在工业厌氧反应器中木质纤维素预处理的新方向。

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引用次数: 0

The influence and correlation analysis of environmental factors and saturation index on the calcium carbonate precipitation induced by carbonic anhydrase-producing bacteria 环境因子和饱和指数对产碳酸酐菌诱导碳酸钙沉淀的影响及相关分析

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-13 DOI: 10.1016/j.procbio.2026.01.010

Jiahua Zhu , Jing Sun , Nan Hu , Zhihui Yang , Aishu Li , Shunchao Yao , Guangyue Li

Microbially induced carbonate precipitation (MICP) has garnered widespread attention as a promising biomineralization technology. However, existing studies predominantly focus on single-factor effects, lacking systematic analysis of multi-factor interactions and mineralization kinetics. This study systematically investigated the dynamic influences of environmental factors on the mineralization process and precipitation characteristics induced by a carbonic anhydrase-producing strain. Through correlation analysis, a multi-parameter heatmap was constructed to reveal biomineralization kinetic patterns and clarify the role of the saturation index (SI) in linking microbial metabolism with precipitation characteristics. The results indicated that both precipitation yield and SI exhibit dynamic variations during biomineralization. Under the optimized conditions of 1.0 OD₆₀₀ bacterial solution (BS), 0.2 M cementation solution (CS), 30°C and pH 10, precipitation yield and SI peaked at 3.85 g/L and 3.96, respectively. Higher BS concentration, temperature, pH and SI, combined with a lower CS concentration, favored the formation of regularly shaped calcite. Conversely, lower BS/CS concentrations, pH and SI significantly increased the particle size. Notably, CS concentration and pH exerted more pronounced effects on precipitation characteristics and SI. This study systematically elucidates the kinetic mechanisms of biomineralization and coupling regulatory relationship of environmental factors, providing a theoretical basis for predicting, regulating and optimizing the mineralization process.

微生物诱导碳酸盐沉淀（MICP）作为一种很有前途的生物矿化技术受到了广泛的关注。然而，现有的研究主要集中在单因素作用上，缺乏对多因素相互作用和成矿动力学的系统分析。本研究系统研究了环境因子对产碳酸酐酶菌株诱导的矿化过程和降水特征的动态影响。通过相关分析，构建了多参数热图，揭示了生物矿化动力学模式，阐明了饱和指数（SI）在微生物代谢与降水特征之间的联系。结果表明，在生物矿化过程中，降水产率和SI均呈现动态变化。在1.0 OD600菌液（BS）、0.2 M胶结液（CS）、30℃、pH 10的优化条件下，沉淀得率和SI分别达到3.85 g/L和3.96 g/L。较高的BS浓度、温度、pH和SI，加上较低的CS浓度，有利于形成规则形状的方解石。相反，较低的BS/CS浓度、pH和SI显著增加了粒径。其中，CS浓度和pH对降水特征和SI的影响更为显著。本研究系统阐明了生物矿化的动力学机制和环境因子的耦合调控关系，为矿化过程的预测、调控和优化提供了理论依据。

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引用次数: 0

Evaluation of tetrahydrocurcumin-loaded hybrid calcium carbonate microcrystals for enhanced collagen synthesis 负载四氢姜黄素的杂化碳酸钙微晶促进胶原合成的评价

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-13 DOI: 10.1016/j.procbio.2026.01.008

Ee Taek Hwang , Yeahwa Yoon , Ka Ram Kim , Jinhee Jeong , Jung Soo Kim , Dongwon Kim , Seong-Wook Seo , In-Soo Yoon

Plant-derived natural product-encapsulated organic–inorganic hybrid materials have shown promise as biologically active agents for health. Here, we extend our previous work by investigating the collagen synthesis activity and stability of tetrahydrocurcumin (THC) in amphiphilic copolymer-incorporated calcium carbonate biomineral formulations, offering a novel comparison with curcumin (CUR). The encapsulated THC preserved over 90 % of its initial concentration over four weeks under different storage conditions, confirming its high stability. Interestingly, THC induced 1.58- and 1.55-fold higher COL1A1 expression in normal human dermal fibroblasts (NHDFs) than CUR, enhancing collagen synthesis–related gene expression when 500 and 1000 µg mL⁻¹ hybrid microcrystals were used. These findings contrast with general assumptions that CUR exhibits superior therapeutic effects, illustrating the challenges in predicting the effectiveness of encapsulation technologies. This is the first study to systematically compare the encapsulation and biological efficiencies of CUR and THC in organic–inorganic hybrid materials, contributing to the broader field of natural product-based therapeutics.

植物源性天然产品包封的有机-无机杂化材料已显示出作为健康生物活性剂的前景。在这里，我们扩展了之前的工作，研究了两亲共聚物-碳酸钙生物矿物配方中四氢姜黄素（THC）的胶原合成活性和稳定性，并与姜黄素（CUR）进行了新的比较。包封后的四氢大麻酚在不同的储存条件下保存了4周以上，其浓度保持在初始浓度的90 %以上，证实了其高稳定性。有趣的是，当使用500和1000 µg mL−1杂交微晶时，THC诱导正常人真皮成纤维细胞（NHDFs）中COL1A1的表达比CUR高1.58倍和1.55倍，增强胶原合成相关基因的表达。这些发现与一般认为CUR具有优越的治疗效果的假设形成对比，说明了预测包封技术有效性的挑战。这是第一个系统地比较有机-无机杂化材料中CUR和THC的包封性和生物效率的研究，为更广泛的天然产物治疗领域做出了贡献。

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引用次数: 0

Boosting Arthrospira platensis metabolism during biogas upgrading via carbon coated iron-based nanoparticle addition 通过添加碳包覆铁基纳米颗粒促进沼气升级过程中平台节螺旋体的代谢

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-12 DOI: 10.1016/j.procbio.2026.01.004

Chrysa Anagnostopoulou , Laura Vargas-Estrada , Panagiotis G. Kougias , Raúl Muñoz

Carbon-coated iron-based nanoparticles have demonstrated a significant potential to enhance the performance of biological processes, particularly microalgal cultivation. In this study, the effect of two types of commercially available iron-based nanoparticles, namely CALPECH and SMALLOPS, was evaluated on Arthrospira platensis metabolism during biogas upgrading in batch cultures. Both nanoparticles enhanced CO₂ capture, O₂ production, cyanobacterial growth and phycocyanin synthesis across all tested concentrations. However, the highest phycocyanin content (180 mg·g⁻¹) was reached by the addition of CALPECH nanoparticles at 100 mg·L⁻¹. Further experiments under stress conditions, including increased light intensity (300 and 600 μmol·m⁻²·s⁻¹) and salinity (0.1–0.5 M NaCl) confirmed the beneficial effects of CALPECH. At 600 μmol·m⁻²·s⁻¹ CALPECH nanoparticles enhanced biomass productivity and increased CO₂ capture by 33 % while maintaining phycocyanin content at 178 mg·g⁻¹. Moreover, the addition of 100 mg·L⁻¹ of CALPECH nanoparticles at 0.1 M NaCl slightly improved biogas upgrading performance and increased phycocyanin content to 192.7 mg·g⁻¹. In this context, increasing salinity to 0.5 M caused stress in Arthrospira platensis cells, reducing photosynthetic efficiency regardless of nanoparticle addition. These outcomes highlight the potential of carbon-coated iron-based nanoparticles to improve Arthrospira platensis growth and pigment production, which would ultimately increase the techno-economic feasibility of photosynthetic biogas upgrading.

碳包覆的铁基纳米颗粒在提高生物过程，特别是微藻培养的性能方面显示出巨大的潜力。在本研究中，研究了两种市售铁基纳米颗粒（CALPECH和SMALLOPS）在间歇培养过程中对platarthrospira代谢的影响。这两种纳米颗粒增强了二氧化碳捕获，氧气生产，蓝藻生长和藻蓝蛋白合成在所有测试浓度。而加入浓度为100 mg·L−1的CALPECH纳米颗粒藻蓝蛋白含量最高，为180 mg·g−1。在胁迫条件下，增加光照强度（300和600 μmol·m−2·s−1）和盐度（0.1-0.5 m NaCl），证实了CALPECH的有益作用。在600 μmol·m−2·s−1浓度下，CALPECH纳米颗粒提高了生物量生产力，增加了33 %的CO2捕集量，同时藻蓝蛋白含量保持在178 mg·g−1。此外，在0.1 M NaCl条件下，添加100 mg·L−1的CALPECH纳米颗粒略微提高了沼气的升级性能，藻蓝蛋白含量提高到192.7 mg·g−1。在这种情况下，将盐度增加到0.5 M会导致平节螺旋藻细胞产生应激，无论纳米颗粒是否加入都会降低光合效率。这些结果突出了碳包覆铁基纳米颗粒改善platarthrospira生长和色素生产的潜力，这将最终提高光合沼气升级的技术经济可行性。

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引用次数: 0

Encapsulation promotes the anti-osteoporosis effects of vitamin D3 with enhanced bioavailability and activation efficiency 胶囊化促进维生素D3的抗骨质疏松作用，提高生物利用度和活化效率

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-11 DOI: 10.1016/j.procbio.2026.01.006

Weifeng Hu , Min Zhu , Yihan Wen , Yi Guo , Pei Qiao

Vitamin D3 (Cholecalciferol, Chc) plays a critical role in the prevention and treatment of osteoporosis. However, Chc’s clinical and nutritional applications are substantially limited by poor aqueous solubility, low stability, and inadequate bioavailability. In this study, we developed a nano-micellar delivery system by encapsulating Chc with disodium glycyrrhizin (Na₂GA) to form Chc-Na₂GA micelles, aiming to overcome these limitations. The resulting formulation exhibited markedly improved solubility, enhanced physicochemical stability, increased bioavailability, and a higher hepatic accumulation rate. Importantly, Chc-Na₂GA treatment significantly enhanced anti-osteoporotic efficacy, accompanied by the upregulation of key osteogenesis-related genes, including bmp2a, bump4, col2a, runx2a, and sp7. Structural analysis revealed that Chc was present in an amorphous state within the micelles, stabilized primarily through hydrogen bonding and Pi-alkyl interactions. Overall, this study demonstrates that Na₂GA-based nano-micellization is an effective strategy to improve the delivery and biological efficacy of hydrophobic vitamin D3, highlighting its potential significance for pharmaceutical and food applications in osteoporosis management.

维生素D3 （Cholecalciferol, Chc）在预防和治疗骨质疏松症中起着至关重要的作用。然而，Chc的临床和营养应用在很大程度上受到水溶性差、稳定性低和生物利用度不足的限制。在这项研究中，我们开发了一种纳米胶束递送系统，通过将Chc包裹在甘草酸二钠（Na2GA）中形成Chc-Na2GA胶束，旨在克服这些局限性。所得到的配方表现出明显改善的溶解度，增强的物理化学稳定性，提高的生物利用度和更高的肝脏积累率。重要的是，Chc-Na2GA治疗显著增强了抗骨质疏松的疗效，同时上调了关键的成骨相关基因，包括bmp2a、bump4、col2a、runx2a和sp7。结构分析表明，Chc在胶束内以无定形存在，主要通过氢键和pi -烷基相互作用稳定。综上所述，本研究表明基于na2ga的纳米胶束化是改善疏水性维生素D3的递送和生物功效的有效策略，突出了其在骨质疏松症治疗中的潜在应用价值。

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引用次数: 0

Mitigation of antibiotic-resistant bacteria isolated from hospital effluent by lytic bacteriophage infection 通过溶解噬菌体感染减少医院流出物中分离的耐药细菌

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-09 DOI: 10.1016/j.procbio.2026.01.001

Hayeon Lee, Jeongdong Choi

Antibiotic-resistant bacteria (ARB) released from hospital wastewater pose a significant risk to public health and receiving water bodies. This study evaluated the applicability of phage-based biocontrol for selectively suppressing hospital-derived ARB. Raoultella spp. isolated from treated hospital wastewater exhibited multidrug resistance, including resistance to β-lactams, colistin, and vancomycin. A lytic bacteriophage (Raoul-phage) infecting the isolated ARB was characterized, and its stability was confirmed across a broad range of temperatures, pH levels, and toxic chemical exposures. Batch-reactor assays demonstrated that the abundance of Raoultella spp. was reduced from 75 % to 38 % within 24 h, indicating effective host-specific lytic activity. These results highlight the feasibility of employing lytic bacteriophages for targeted ARB mitigation in hospital wastewater and suggest potential applicability for integration or retrofitting within existing wastewater treatment systems.

医院废水释放的耐药细菌（ARB）对公共卫生和接收水体构成重大风险。本研究评估了基于噬菌体的生物防治在选择性抑制医院源性ARB中的适用性。从处理过的医院废水中分离的Raoultella spp表现出多药耐药，包括对β-内酰胺类、粘菌素和万古霉素的耐药。对感染分离的ARB的裂解噬菌体（Raoul-phage）进行了表征，并在广泛的温度、pH值和有毒化学物质暴露范围内证实了其稳定性。批反应器实验表明，Raoultella spp.丰度在24 h内从75 %降低到38 %，表明有效的宿主特异性裂解活性。这些结果强调了在医院废水中使用裂解噬菌体靶向缓解ARB的可行性，并表明在现有废水处理系统中集成或改造的潜在适用性。

引用次数: 0

Revealing and manipulating the LmbU-mediated connection between lincomycin production and the ectABCD gene cluster in S. lincolnensis 揭示和操纵lmbu介导的林肯葡萄球菌林可霉素生产与ectABCD基因簇之间的联系

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-01-07 DOI: 10.1016/j.procbio.2026.01.002

Yue Mao , Jiang Ye , Ruida Wang , Haizhen Wu , Huizhan Zhang

Lincomycin, an antibiotic produced by Streptomyces lincolnensis, is widely used to treat Gram-positive bacterial and anaerobic infections. This study found that LmbU, a positive regulatory factor within the lincomycin biosynthesis gene cluster, can positively regulate the extracellular target gene cluster ectABCD (ect), which is involved in the synthesis of ectoine/hydroxyectoine. By knocking out ect, the lincomycin production was increased 2.8-fold. The addition of exogenous L-Asp in both xbl1 and Δect strains confirmed that the increased lincomycin production in Δect was due to enhanced precursor supply. Furthermore, osmotic stress experiments demonstrated that moderate NaCl concentrations could increase both ect expression and lincomycin production, while higher osmotic pressures resulted in decreased production and biomass. Together, these findings provide new mechanistic insights and offer a theoretical basis for the rational design of high-production industrial strains.

林可霉素是一种由林肯链霉菌产生的抗生素，广泛用于治疗革兰氏阳性细菌和厌氧感染。本研究发现LmbU是林可霉素生物合成基因簇内的正调控因子，可以正调控参与外托因/羟外托因合成的细胞外靶基因簇ectABCD （ect）。通过敲除等，林可霉素的产量提高了2.8倍。在xbl1和Δect菌株中添加外源L-Asp证实Δect菌株林可霉素产量的增加是由于前体供应的增加。此外，渗透胁迫实验表明，中等NaCl浓度可以增加ect表达和林可霉素产量，而较高的渗透压会导致林可霉素产量和生物量下降。总之，这些发现提供了新的机理见解，并为合理设计高产工业菌株提供了理论基础。

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引用次数: 0