Process Biochemistry最新文献_第6页

Screening and mechanistic study of chicken embryo egg-yolk peptide-Fe²⁺ chelates based on Caco-2 cells 基于Caco-2细胞的鸡胚蛋黄肽- fe 2 +螯合物的筛选及机制研究

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-12-01 DOI: 10.1016/j.procbio.2025.12.001

Jia Lin , Shuaishuai Wei , Shijun Li , Donglei Zheng , Ziwei Zhang , Shijian Dong , Shugang Li , Lulu Ma

The iron-chelating peptides were screened from egg yolk, and the structure, and mechanism were investigated. By examining the incubation stages, day 12 had the highest iron chelation rate of 61.55 % ±1.69 %. On this basis, the peptide sequence PQETPPDTT (PT-9) was screened according to its molecular weight, amino acid composition, and molecular docking (binding energy < −4.2 kcal/mol). Compared with PT-9, the surface charge and average size of PT-9-Fe²⁺ changed from −17.07 mV and 1142 nm to −6.64 mV and 444 nm, respectively. Isothermal titration calorimetry (ITC) and Kinetic simulations revealed that the Gln2, Thr4, Asp7, and Thr9 in PT-9 formed a stable eight-coordination bond network spontaneously (ΔH = −1.689 kJ/mol) with Fe²⁺ via CO, N-H, and three water molecules, with an Fe^2 + release rate lower than 25 % in the gastrointestinal digestive tests. PT-9-Fe²⁺ enhanced Fe²⁺ uptake to 2.0-fold in Caco-2 cells compared with FeSO₄. Through a combination of methods, including RNA-seq and inhibitors, it was demonstrated that PT-9-Fe²⁺ up-regulated the expression of genes encoding Divalent Metal Transporter 1 (DMT1) and Transferrin Receptor (TFRC), and down-regulated the expression of genes encoding tight junction proteins and adhesion proteins. The iron uptake pathways mediated by PT-9-Fe²⁺ were predominantly paracellular transport, endocytosis and DMT1.

从蛋黄中筛选铁螯合肽，并对其结构和作用机理进行了研究。通过观察孵育期，第12天铁螯合率最高，为61.55 %±1.69 %。在此基础上，根据肽序列PQETPPDTT （PT-9）的分子量、氨基酸组成、分子对接（结合能<；−4.2 kcal/mol）筛选肽序列PQETPPDTT （PT-9）。与PT-9相比，PT-9- fe 2 +的表面电荷和平均尺寸分别从−17.07 mV和1142 nm变为−6.64 mV和444 nm。等温滴定量热法（ITC）和动力学模拟表明，PT-9中的Gln2、Thr4、Asp7和Thr9以Fe2 +通过CO、N-H和3个水分子自发形成稳定的八配位键网络（ΔH =−1.689 kJ/mol），胃肠道消化测试中Fe2 +的释放率低于25 %。与FeSO4相比，PT-9-Fe2+在Caco-2细胞中将Fe2+的吸收量提高到2.0倍。通过RNA-seq和抑制剂等综合方法，证实PT-9-Fe2+上调编码二价金属转运蛋白1 （DMT1）和转铁蛋白受体（TFRC）的基因表达，下调编码紧密连接蛋白和粘附蛋白的基因表达。PT-9-Fe2+介导的铁摄取途径主要是细胞旁转运、胞吞作用和DMT1。

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引用次数: 0

Heterologous N-glycosyltransferase expression reprograms the glycoproteome and functional profile of Bacillus subtilis WB800N 异源n -糖基转移酶的表达对枯草芽孢杆菌WB800N的糖蛋白组和功能谱进行了重编程

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-12-14 DOI: 10.1016/j.procbio.2025.12.008

Zhaoxi Liu, Yue Yu, Yupin Nie, Jiangyu Yang, Min Chen

Engineering bacterial glycosylation represents a promising strategy to modulate protein function and cellular physiology. In this study, we introduced a heterologous N-glycosyltransferase (AaNGT) into Bacillus subtilis WB800N to explore its impact on the host glycoproteome and functional profile. The recombinant strain successfully expressed and purified AaNGT, which exhibited in vitro activity toward UDP-Gal and UDP-Glc, as confirmed by TLC and HPLC analyses. Comparative glycoproteomic profiling revealed that AaNGT expression substantially increased N-glycosylation events, diversifying glycoprotein targets across metabolic enzymes, stress response proteins, and structural components. Functional annotation indicated enrichment in amino acid metabolism, secondary metabolite biosynthesis, stress and detoxification pathways, and signal transduction processes, suggesting that protein glycosylation remodels host metabolic and regulatory networks. Moreover, engineered glycosylation enhanced adhesion to Caco-2 cells and improved tolerance to simulated gastric and intestinal conditions, highlighting potential benefits for strain stability and survivability. Together, these findings demonstrate that heterologous NGT-mediated glycosylation can reprogram the glycoproteome and functional landscape of B. subtilis, providing a versatile platform for engineering Gram-positive chassis strains with tailored metabolic and physiological traits.

工程细菌糖基化代表了一种有前途的策略来调节蛋白质功能和细胞生理。本研究将异源n -糖基转移酶（AaNGT）导入枯草芽孢杆菌WB800N，探讨其对宿主糖蛋白组和功能谱的影响。重组菌株成功表达并纯化了AaNGT，经薄层色谱和高效液相色谱分析证实，AaNGT对UDP-Gal和UDP-Glc具有体外活性。比较糖蛋白组学分析显示，AaNGT表达显著增加了n -糖基化事件，使糖蛋白靶点在代谢酶、应激反应蛋白和结构组分中多样化。功能注释表明，在氨基酸代谢、次生代谢物生物合成、应激和解毒途径以及信号转导过程中富集，表明蛋白质糖基化重塑了宿主的代谢和调节网络。此外，工程糖基化增强了Caco-2细胞的粘附，提高了对模拟胃和肠道条件的耐受性，突出了菌株稳定性和生存能力的潜在益处。总之，这些发现表明，异源ngt介导的糖基化可以重新编程枯草芽孢杆菌的糖蛋白组和功能景观，为具有定制代谢和生理特性的革兰氏阳性底盘菌株的工程提供了一个通用平台。

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引用次数: 0

Design and optimization of a cyanide dihydratase-cross-linked enzyme aggregate system for enhanced biodegradation of cyanide: Integrating statistical and machine learning approaches 氰化物二水合酶-交联酶聚合系统的设计与优化，以增强氰化物的生物降解：整合统计和机器学习方法

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.procbio.2025.11.012

Ademakinwa A. Nelson , Agunbiade M. Oladele , Adeyanju M. Moronkeji , Alli Kazeem , Ayinla Z. Adenike

This study developed a robust cyanide biodegradation system using cross-linked enzyme aggregates (CLEAs) of cyanide dihydratase from Aureobasidium pullulans NAC8 (ApCynD). The intracellular enzyme was immobilized as cynD-CLEA and evaluated alongside the free enzyme. Immobilization significantly improved operational stability, with over 90 % activity retained after seven reuse cycles and 80 % after six weeks of storage. Structural analysis showed increased α-helix (29.4–38.8 %) and β-turn (12.6 % to 18.4 %) contents, with reductions in β-sheet and random coil, indicating a more ordered conformation. Kinetic profiling revealed elevated V_max and catalytic efficiency despite a higher K_m. Process conditions were optimized using response surface methodology (RSM, R² = 0.902) and artificial neural networks (ANN, R² = 0.958). RSM identified cyanide concentration as the most significant factor, with optimal conditions (1000 ppm, 150 rpm, 1000 mg CLEA, 3 h) yielding 82 % removal. ANN-predicted optimums (240 ppm, 200 rpm, 865 mg CLEA, 6 h) achieved 90.5 % removal, confirming better performance in modeling nonlinear interactions. SHAP analysis enhanced model interpretability. The integrated RSM-ANN approach offers a powerful strategy for optimizing enzyme-based bioremediation, with cynD-CLEA showing strong potential for industrial application. Further studies are recommended to assess broader conditions and extended operational cycles.

本研究利用普鲁兰短毛霉NAC8 （ApCynD）氰化物二水合酶的交联酶聚集体（CLEAs）建立了一个强大的氰化物生物降解系统。将胞内酶固定为cynD-CLEA，并与游离酶一起进行评价。固定化显著提高了操作稳定性，在7个重复使用周期后保持了超过90% %的活性，在6周后保持了80% %的活性。结构分析表明，α-螺旋（29.4 ~ 38.8 %）和β-转（12.6 ~ 18.4 %）含量增加，β-片和随机线圈含量减少，构象更加有序。动力学分析显示，尽管Km增加，但Vmax和催化效率也有所提高。采用响应面法（RSM, R²= 0.902）和人工神经网络（ANN, R²= 0.958）对工艺条件进行优化。RSM发现氰化物浓度是最重要的影响因素，最佳条件为1000 ppm， 150 rpm, 1000 mg CLEA, 3 h，去除率为82% %。ann预测的最优值（240 ppm, 200 rpm, 865 mg CLEA, 6 h）的去除率达到了90.5 %，证实了建模非线性相互作用的更好性能。SHAP分析增强了模型的可解释性。综合RSM-ANN方法为优化酶基生物修复提供了强有力的策略，cynD-CLEA显示出强大的工业应用潜力。建议进行进一步研究，以评估更广泛的条件和延长的业务周期。

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引用次数: 0

Optimization of pretreatment methods for banana leaf waste and its kinetics studies for effective biogas generation by anaerobic co-digestion with food waste 香蕉叶废弃物预处理方法优化及与厨余厌氧共消化产气动力学研究

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.procbio.2025.11.009

Athithyan Ilangovan , Sri Bala Kameswari Kanchinadham

Enhancing enzymatic hydrolysis and biofuel production requires effective biomass pretreatment. This study systematically optimised four pretreatment methods like dilute acid, alkali, molecular solvent, and thermal to maximise reducing sugars yield from lignocellulosic biomass. Key parameters like concentration, temperature, and residence time were varied. The highest reducing sugars yield (8000–10000 mg/L) was achieved with alkali pretreatment (4 % w/v at 100 °C for 30 min), indicating significant cellulose breakdown. molecular solvent and thermal pretreatments produced moderate yields but were promising. The kinetic constant (k) calculated for dilute acid, alkali, molecular solvent, and thermal pretreatments were 0.02726 ± 0.00643 min⁻¹ , 0.02235 ± 0.00584 min⁻¹ , 0.02077 ± 0.0088 min⁻¹ and 0.02218 ± 0.01672 min⁻¹ respectively, using first order kinetics helped to understand the reaction rate and degree of hydrolysis. Biogas generation was modelled using the modified Gompertz equation. Kinetic modelling studies revealed that co-digestion of food waste with alkali-pretreated banana leaf waste led to maximum biogas yield, with a maximum production rate (R_m) of 322.06 ± 51.51 mL/day. This study provides a foundation for scalable biomass valorisation, offering key insights into tailoring pretreatment strategies to enhance hydrolysis, sugar release, and biogas production from lignocellulosic waste.

提高酶解和生物燃料生产需要有效的生物质预处理。本研究系统优化了稀酸、碱、分子溶剂和热四种预处理方法，以最大限度地提高木质纤维素生物质的还原糖产量。关键参数如浓度、温度和停留时间是不同的。碱预处理（4 % w/v, 100°C, 30 min）可获得最高的还原糖产率（8000-10000 mg/L），表明纤维素分解显著。分子溶剂和热预处理产率适中，但前景广阔。动力学常数(k)计算稀酸、碱、溶剂分子,和热预处理0.02726 ±0.00643  分钟⁻¹ , 0.02235±0.00584  分钟⁻¹ , 0.02077±0.0088  分钟⁻¹  和0.02218±0.01672  分钟⁻¹ 分别使用一阶动力学帮助理解反应速率和水解度。利用修正的Gompertz方程对沼气生成进行了建模。动力学模型研究表明，食物垃圾与经碱预处理的香蕉叶垃圾共消化可产生最大的沼气量，最大产气量（Rm）为322.06±51.51 mL/d。该研究为可扩展的生物质增值提供了基础，为定制预处理策略提供了关键见解，以增强木质纤维素废物的水解，糖释放和沼气生产。

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引用次数: 0

Economic and technical insights into carotenoids and lipids large-scale production in Rhodotorula glutinis: A study based on pulse-feeding culture, cell disruption strategies and cytotoxicity 粘红酵母类胡萝卜素和脂类大规模生产的经济和技术见解：一项基于脉冲饲养培养、细胞破坏策略和细胞毒性的研究

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-12-04 DOI: 10.1016/j.procbio.2025.12.002

Carolina Ramírez-Martínez , Susana Rincón , Alejandro Zepeda , Alberto Ordaz

The oleaginous yeast Rhodotorula glutinis is a promising microorganism for simultaneous production of carotenoids and lipids which are traditionally derived from plants and chemical synthesis; however, their production faces challenges such as stability and health issues, supply consistency, and large-scale production studies. This study focused on the use of Rhodotorula glutinis with a pulse-feeding culture strategy in a stirred tank reactor to intensify the biomass concentration (52.84 ± 0.5 g/L). Effective cell disruption for products extraction was achieved using acid treatment and cold solvent extraction, yielding 3.21 ± 0.5 mg/g dry yeast carotenoids and 0.175 ± 0.01 g/g dry yeast lipids. A safety test on human fibroblasts showed no cytotoxic effects in the obtained extract. Using experimental results as input parameters, three simulation scenarios were constructed in SuperPro Designer, with a product composed of lipids (97.41 % w/w) and carotenoids (2.59 % w/w): Scenario 1 (500 kg/year), Scenario 2 (1000 kg/year), and Scenario 3 (2000 kg/year). Adequate selection of equipment size can reduce both operational and capital expenses. Revenues were mainly linked to lipid production due to higher mass yields. Future work should focus on process scalability, feeding regime optimization, medium formulation, extract composition and functionality, and environmental impact assessment.

产油酵母粘红酵母（Rhodotorula glutinis）是一种很有前途的微生物，可以同时生产类胡萝卜素和脂类，这两种物质传统上是从植物和化学合成中获得的；然而，它们的生产面临着稳定性和健康问题、供应一致性和大规模生产研究等挑战。本研究主要研究了在搅拌槽式反应器中采用脉冲投料培养方法提高粘红酵母的生物量浓度（52.84 ± 0.5 g/L）。通过酸处理和冷溶剂萃取实现了有效的细胞破坏，得到3.21 ± 0.5 mg/g干酵母类胡萝卜素和0.175 ± 0.01 g/g干酵母脂质。对人成纤维细胞的安全性试验表明，所获得的提取物没有细胞毒性作用。以实验结果为输入参数，在SuperPro Designer中构建了三种模拟场景，其中产品由脂质（97.41 % w/w）和类胡萝卜素（2.59 % w/w）组成：场景1（500 kg/年）、场景2（1000 kg/年）和场景3（2000 kg/年）。适当选择设备尺寸可以减少运营和资本支出。由于产量较高，收入主要与油脂生产有关。未来的工作应集中在工艺可扩展性、投料制度优化、培养基配方、提取物组成和功能以及环境影响评价等方面。

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引用次数: 0

Enhanced adsorption capacity of mesoporous composite agarose beads for glycoprotein separation: Experiments and mathematical modeling as a prerequisite for scale-up 用于糖蛋白分离的介孔复合琼脂糖珠的增强吸附能力：实验和数学建模作为扩大规模的先决条件

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-12-10 DOI: 10.1016/j.procbio.2025.12.005

Aref Honarparvaran , Mohammad Reza Mehrnia , Fatemeh Pourasgharian Roudsari

Affinity chromatography is an economic bottleneck in scaling downstream bioprocesses. This study employs experimental and modeling methodologies to examine key parameters influencing the adsorption capacity of a medium-sized glycoprotein on boronate affinity column. To investigate the impact of intraparticle nanostructure on adsorption capacity, Composite Beads were fabricated, exhibiting enhanced porosity (30 %) and mechanical robustness (18 %). The increased dynamic binding capacity (DBC) of columns suggests facilitated pore diffusion within Composite Beads’ mesoporous structure. Mathematical modeling using the General Rate Model (GRM) and the Pore Diffusion Model (PDM) was utilized to predict the columns’ breakthrough curves and the beads’ saturation rate, respectively. PDM revealed a relatively prolonged saturation of Composite Beads compared to Agarose Beads. Evaluating the influence of column dimensions on the DBCs elucidated that enlarging the column diameter wielded a more pronounced impact (15 %) than altering the column length (9 %), highlighting the importance of overcoming intraparticle diffusion resistance. The non-equilibrium-based GRM, not only predicted breakthrough curves of columns with varying dimensions (Absolute Average Deviation of 7.63 %) but also the intricate behavior of columns packed with Beads possessing diverse adsorption capacities. The outcomes of this study provided valuable insights for the prospective scale-up of nanostructured beads in affinity chromatography columns.

亲和色谱法是扩大下游生物工艺规模的经济瓶颈。本研究采用实验和建模方法，研究了影响中型糖蛋白在硼酸盐亲和柱上吸附能力的关键参数。为了研究颗粒内纳米结构对吸附能力的影响，制备了具有增强孔隙率（30 %）和机械鲁棒性（18 %）的复合微珠。柱的动态结合能（DBC）的增加表明复合微珠介孔结构内的孔隙扩散更容易。采用通用速率模型（General Rate Model， GRM）和孔隙扩散模型（Pore Diffusion Model， PDM）进行数学建模，分别预测了柱的突破曲线和球的饱和速率。与琼脂糖珠相比，PDM显示复合珠的饱和时间相对较长。评估柱尺寸对DBCs的影响表明，扩大柱直径比改变柱长（9 %）具有更显著的影响（15 %），突出了克服颗粒内扩散阻力的重要性。基于非平衡的GRM不仅预测了不同尺寸柱的突破曲线（绝对平均偏差为7.63 %），而且还预测了具有不同吸附容量的小球填充柱的复杂行为。本研究的结果为亲和色谱柱中纳米结构珠的预期扩大提供了有价值的见解。

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引用次数: 0

Laccase-mediated oxidative polymerization of gallic acid for wool fabric dyeing and functionalization 漆酶介导的没食子酸氧化聚合对羊毛织物的染色及功能化

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-12-14 DOI: 10.1016/j.procbio.2025.12.010

Xue Han , Dong Han , Aixue Dong , Jinghao Cai , Jing Shen , Runbo Wang

Laccase-catalyzed gallic acid polymerization represents a clean, in-situ dyeing method for wool fabrics that addresses the pollution issues in traditional dyeing processes. This study employs four dyeing processes to achieve laccase-catalyzed polymerization of gallic acid on wool fabrics, systematically comparing the effects of the different processes on dyeing performance and functional properties. The results demonstrate that the pre-polymerization and subsequent dyeing method produced the deepest color (K/S = 5.33) and the highest level of UV protection (UPF = 306.32) of all the processes. All dyed fabrics exhibited good color fastness (≥ grade 3) and enhanced functionality, including improved tensile strength and increased hydrophilicity, albeit with reduced alkali resistance. Spectroscopic analysis confirms the formation of covalent bonds between wool fibers and polymerized gallic acid via laccase-mediated oxidation. These findings highlight advancements in the enzymatic polymerization of phenolic compounds for colored textile surfaces and offer potential applications in enzymatic dyeing and sustainable textile processing.

漆酶催化没食子酸聚合代表了一种清洁的羊毛织物原位染色方法，解决了传统染色工艺中的污染问题。本研究采用四种染色工艺在羊毛织物上实现漆酶催化没食子酸聚合，系统比较不同染色工艺对羊毛织物染色性能和功能性能的影响。结果表明，预聚合后染色法的颜色最深（K/S = 5.33），紫外线防护水平最高（UPF = 306.32）。所有染色织物都表现出良好的色牢度（≥3级）和增强的功能性，包括提高抗拉强度和亲水性，尽管耐碱性降低。光谱分析证实羊毛纤维和聚合没食子酸之间通过漆酶介导的氧化形成共价键。这些发现突出了酶促聚合酚类化合物用于有色纺织品表面的进展，并在酶促染色和可持续纺织品加工中提供了潜在的应用。

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引用次数: 0

Multistory interface fermentor coupled with an adsorption unit: Application to the production of a biologically active azaphilone metabolite, (+)-sclerotiorin, with Penicillium sclerotiorum NBRC 113796 结合吸附装置的多层界面发酵罐：应用于菌核青霉NBRC 113796生产具有生物活性的氮唑啉代谢物(+)-菌核苷

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-12-27 DOI: 10.1016/j.procbio.2025.12.017

Shinobu Oda , Fuka Kumazaki , Riko Murakami , Yoshihiro Watanabe , Masato Iwatsuki

A novel fermentation system, interface fermentor (IFF), was constructed and applied to the production of (+)-sclerotiorin (SCL) which was a fungal secondary metabolite having various biological activities. The IFF was composed of a nutrient agar plate (bottom phase), a fungal mat (middle phase), and a hydrophobic organic solvent (top phase). Six fermentor units stacked were connected by oil-tolerant rubber tubes to form a fermentor tower. Two fermentor towers were jointed in parallel to an adsorption unit packed with adsorbent granules (silica gel). While an organic solvent, low-density dimethylsilicone oil, in the units overflowed into right under ones, the solvent in both bottom units overflowed into the adsorption unit. The solvent in the adsorption unit was forwarded to head units in each fermentor tower with a circulation pump. The efficacy of the multi-story IFF system was proved by through the production of SCL with Penicillium sclerotiorum NBRC 113796. The IFF coupled with an adsorption unit, of which total surface area and volume of agar plates were 5508 cm² and 10.8 L, gave 7.91 g of SCL in total by 4 weeks.

建立了一种新型的发酵体系——界面发酵罐（IFF），并将其应用于真菌次生代谢产物(+)-菌核蛋白（SCL）的生产。IFF由营养琼脂板（底相）、真菌垫（中相）和疏水有机溶剂（上相）组成。六个发酵罐单元堆叠在一起，由耐油橡胶管连接，形成一个发酵罐塔。两个发酵罐塔平行连接到一个吸附单元，吸附单元中填充了吸附剂颗粒（硅胶）。当单元中的有机溶剂低密度二甲基硅油溢出到正下方单元时，两个底部单元中的溶剂溢出到吸附单元中。吸附装置中的溶剂通过循环泵输送到每个发酵塔的头装置。利用菌核青霉NBRC 113796生产SCL，验证了多层IFF系统的有效性。IFF与吸附单元（琼脂板的总表面积和体积分别为5508 cm2和10.8 L）相结合，在4周内共获得7.91 g的SCL。

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引用次数: 0

Gradient voltages stimulation of biogenic coalbed methane: Insights into the evolution of distinct habitats 梯度电压对生物成因煤层气的刺激作用：对不同生境演化的洞察

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-12-14 DOI: 10.1016/j.procbio.2025.12.011

Yifan Yin , Zhicheng Shi , Jiayan Zhang , Xiao Feng , Hongguang Guo , Zaixing Huang , Michael Urynowicz

Microbial electrolysis cells (MECs) could enhance anaerobic biodegradation of coal, thereby facilitating biogenic methane production. This study investigated the effects of gradient voltages (0 V-1.5 V) on coal-to-methane conversion. The changes in methane production, current, microbial communities, coal structure and liquid-phase organics were analyzed. Methane production peaked at 330.63 µmol/g coal at 1.2 V, increased by 31.4 % compared with 0 V. Effective voltage generated current peaks in early stage, facilitating organics oxidation. High-throughput sequencing and quantitative PCR revealed that Pseudomonas and Enterobacter were dominant bacteria in sediment, suspension, anode, and the maximum bacterial biomass in sediment and suspension increased by 66.1 % and 47.4 %, respectively. Methanosarcina and Bathyarchaeia in suspension were main archaea for methane production, with total biomass increasing by 46.8 % at 0.6 V and 83.4 % at 1.2 V. FTIR and GC-MS results confirmed that voltage stimulation accelerated coal biodegradation, utilized aromatic substituents and oxygen-containing groups, increased aliphatic branching and enhanced transformation of hydrocarbons. Overall, applied voltages could enhance methane production, with 0.6 V and 1.2 V showing more pronounced effects. This was achieved by providing electrons, increasing biomass, and promoting degradation. The findings offered theoretical guidance for the application of MECs to enhance coal-to-methane conversion.

微生物电解细胞（MECs）可以促进煤的厌氧生物降解，从而促进生物甲烷的生产。本研究考察了梯度电压（0 V-1.5 V）对煤制甲烷转化的影响。分析了甲烷产量、电流、微生物群落、煤结构和液相有机物的变化。甲烷产量在1.2 V时达到峰值，为330.63µmol/g煤，比0 V时增加了31.4% %。有效电压产生的电流在早期达到峰值，有利于有机物氧化。高通量测序和定量PCR结果显示，沉积物、悬浮液和阳极中的优势菌为假单胞菌和肠杆菌，沉积物和悬浮液中的最大细菌生物量分别增加了66.1% %和47.4% %。悬浮菌中产甲烷的主要古菌为甲烷生菌（Methanosarcina）和深海生菌（Bathyarchaeia），在0.6 V和1.2 V下总生物量分别增加46.8 %和83.4 %。FTIR和GC-MS结果证实，电压刺激加速了煤的生物降解，利用了芳香族取代基和含氧基团，增加了脂肪族分支，促进了碳氢化合物的转化。总的来说，施加电压可以提高甲烷产量，0.6 V和1.2 V的效果更明显。这是通过提供电子、增加生物量和促进降解来实现的。研究结果为mec在提高煤制甲烷转化率方面的应用提供了理论指导。

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引用次数: 0

SoluBat: A bidirectional mamba framework for high-throughput protein solubility prediction in bioprocess optimization SoluBat：用于生物工艺优化中高通量蛋白质溶解度预测的双向曼巴框架

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2026-02-01 Epub Date: 2025-12-11 DOI: 10.1016/j.procbio.2025.12.006

Linjie Wu , Chen Zhang , Zijian Hu , Xurong Wang , Hongrong Liu , Bin Zhu

Protein solubility prediction is a critical step in protein engineering and biomanufacturing, directly influencing wet-lab pre-screening costs and the efficiency of high-throughput R&D. To address the limitations of existing methods in computational efficiency and model interpretability, this study proposes SoluBat, a multimodal deep learning model based on a bidirectional Mamba architecture. SoluBat integrates protein sequence, evolutionary information (PSSM), and physicochemical properties, dynamically fusing these features via an efficient gating mechanism to achieve a balance between performance and energy consumption. Experimental results demonstrate that SoluBat achieves higher accuracy and Matthews correlation coefficient than mainstream deep learning and traditional methods on multiple public datasets, while markedly reducing computational resource consumption. In addition, the model is equipped with built-in per-residue attribution analysis, supporting rational protein design. SoluBat thus provides an efficient and interpretable computational solution for high-throughput protein solubility prediction and industrial applications.

蛋白质溶解度预测是蛋白质工程和生物制造的关键步骤，直接影响湿实验室预筛选成本和高通量研发效率。为了解决现有方法在计算效率和模型可解释性方面的局限性，本研究提出了基于双向Mamba架构的多模态深度学习模型SoluBat。SoluBat集成了蛋白质序列、进化信息（PSSM）和物理化学性质，通过有效的门控机制动态融合这些特征，以实现性能和能耗之间的平衡。实验结果表明，在多个公共数据集上，与主流深度学习和传统方法相比，SoluBat获得了更高的准确率和马修斯相关系数，同时显著降低了计算资源消耗。此外，该模型还内置了每残基属性分析，支持合理的蛋白质设计。因此，SoluBat为高通量蛋白质溶解度预测和工业应用提供了高效和可解释的计算解决方案。

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