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Viscoelastic hydrogel combined with dynamic compression promotes osteogenic differentiation of bone marrow mesenchymal stem cells and bone repair in rats.
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-11-23 eCollection Date: 2025-01-01 DOI: 10.1093/rb/rbae136
Chao Yang, Wenbin Cai, Pan Xiang, Yu Liu, Hao Xu, Wen Zhang, Fengxuan Han, Zongping Luo, Ting Liang

A biomechanical environment constructed exploiting the mechanical property of the extracellular matrix and external loading is essential for cell behaviour. Building suitable mechanical stimuli using feasible scaffold material and moderate mechanical loading is critical in bone tissue engineering for bone repair. However, the detailed mechanism of the mechanical regulation remains ambiguous. In addition, TRPV4 is involved in bone development. Therefore, this study aims to construct a viscoelastic hydrogel combined with dynamic compressive loading and investigate the effect of the dynamic mechanical environment on the osteogenic differentiation of stem cells and bone repair in vivo. The role of TRPV4 in the mechanobiology process was also assessed. A sodium alginate-gelatine hydrogel with adjustable viscoelasticity and good cell adhesion ability was obtained. The osteogenic differentiation of BMSCs was obtained using the fast stress relaxation hydrogel and a smaller compression strain of 1.5%. TRPV4 was activated in the hydrogel with fast stress relaxation time, followed by the increase in intracellular Ca2+ level and the activation of the Wnt/β-catenin pathway. The inhibition of TRPV4 induced a decrease in the intracellular Ca2+ level, down-regulation of β-catenin and reduced osteogenesis differentiation of BMSCs, suggesting that TRPV4 might be the key mechanism in the regulation of BMSC osteogenic differentiation in the viscoelastic dynamic mechanical environment. The fast stress relaxation hydrogel also showed a good osteogenic promotion effect in the rat femoral defect model. The dynamic viscoelastic mechanical environment significantly induced the osteogenic differentiation of BMSCs and bone regeneration, which TRPV4 being involved in this mechanobiological process. Our study not only provided important guidance for the mechanical design of new biomaterials, but also provided a new perspective for the understanding of the interaction between cells and materials, the role of mechanical loading in tissue regeneration and the use of mechanical regulation in tissue engineering.

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引用次数: 0
A microenvironment-adaptive GelMA-ODex@RRHD hydrogel for responsive release of H2S in promoted chronic diabetic wound repair. 一种微环境适应性GelMA-ODex@RRHD水凝胶,用于促进慢性糖尿病伤口修复中H2S的响应释放。
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-11-23 eCollection Date: 2025-01-01 DOI: 10.1093/rb/rbae134
Zhixian Yuan, Wei Zhang, Chang Wang, Chuwei Zhang, Chao Hu, Lu Liu, Lunli Xiang, Shun Yao, Rong Shi, Dejiang Fan, Bibo Ren, Gaoxing Luo, Jun Deng

Chronic diabetic wounds present significant treatment challenges due to their complex microenvironment, often leading to suboptimal healing outcomes. Hydrogen sulfide (H2S), a crucial gaseous signaling molecule, has shown great potential in modulating inflammation, oxidative stress and extracellular matrix remodeling, which are essential for effective wound healing. However, conventional H2S delivery systems lack the adaptability required to meet the dynamic demands of different healing stages, thereby limiting their therapeutic efficacy. To address this, we developed an injectable, ROS-responsive H2S donor system integrated within a gelatin methacryloyl (GelMA) hydrogel matrix, forming a double-network hydrogel (GelMA-ODex@RRHD). The injectability of this hydrogel allows for minimally invasive application, conforming closely to wound contours and ensuring uniform distribution. The incorporation of oxidatively modified dextran derivatives (ODex) not only preserves biocompatibility but also enables the chemical attachment of ROS-responsive H2S donors. The GelMA-ODex@RRHD hydrogel releases H2S in response to oxidative stress, optimizing the environment for cell growth, modulating macrophage polarization and supporting vascular regeneration. This innovative material effectively suppresses inflammation during the initial phase, promotes tissue regeneration in the proliferative phase and facilitates controlled matrix remodeling in later stages, ultimately enhancing wound closure and functional recovery. The H2S released by GelMA-ODex@RRHD not only expedited the process of wound healing but also improved the biomechanical characteristics of newborn skin in diabetic mice, particularly in terms of stiffness and elasticity. This enhancement resulted in the skin quality being more similar to normal skin during the wound healing process. By aligning therapeutic delivery with the natural healing process, this approach offers a promising pathway toward more effective and personalized treatments for chronic diabetic wounds.

由于其复杂的微环境,慢性糖尿病伤口呈现出显著的治疗挑战,往往导致不理想的愈合结果。硫化氢(H2S)是一种重要的气体信号分子,在调节炎症、氧化应激和细胞外基质重塑方面显示出巨大的潜力,这对伤口的有效愈合至关重要。然而,传统的H2S输送系统缺乏适应不同愈合阶段动态需求的适应性,从而限制了其治疗效果。为了解决这个问题,我们开发了一种可注射的ros响应H2S供体系统,该系统集成在明胶甲基丙烯酰(GelMA)水凝胶基质中,形成双网络水凝胶(GelMA-ODex@RRHD)。这种水凝胶的可注射性允许微创应用,密切符合伤口轮廓并确保均匀分布。氧化修饰右旋糖酐衍生物(ODex)的掺入不仅保持了生物相容性,而且使ros反应的H2S供体能够化学附着。GelMA-ODex@RRHD水凝胶在氧化应激下释放H2S,优化细胞生长环境,调节巨噬细胞极化,支持血管再生。这种创新材料在初始阶段有效抑制炎症,在增殖阶段促进组织再生,在后期促进受控的基质重塑,最终促进伤口愈合和功能恢复。GelMA-ODex@RRHD释放的H2S不仅加速了创面愈合过程,而且改善了糖尿病小鼠新生皮肤的生物力学特性,特别是在刚度和弹性方面。这种增强导致皮肤质量在伤口愈合过程中更类似于正常皮肤。通过将治疗传递与自然愈合过程相结合,这种方法为慢性糖尿病伤口的更有效和个性化治疗提供了一条有希望的途径。
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引用次数: 0
Engineered bio-functional material-based nerve guide conduits for optic nerve regeneration: a view from the cellular perspective, challenges and the future outlook. 基于工程生物功能材料的视神经再生神经引导导管:细胞视角、挑战与未来展望
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-11-22 eCollection Date: 2025-01-01 DOI: 10.1093/rb/rbae133
Enoch Obeng, Baoguo Shen, Wei Wang, Zhenyuan Xie, Wenyi Zhang, Zhixing Li, Qinqin Yao, Wencan Wu

Nerve injuries can be tantamount to severe impairment, standard treatment such as the use of autograft or surgery comes with complications and confers a shortened relief. The mechanism relevant to the regeneration of the optic nerve seems yet to be fully uncovered. The prevailing rate of vision loss as a result of direct or indirect insult on the optic nerve is alarming. Currently, the use of nerve guide conduits (NGC) to some extent has proven reliable especially in rodents and among the peripheral nervous system, a promising ground for regeneration and functional recovery, however in the optic nerve, this NGC function seems quite unfamous. The insufficient NGC application and the unabridged regeneration of the optic nerve could be a result of the limited information on cellular and molecular activities. This review seeks to tackle two major factors (i) the cellular and molecular activity involved in traumatic optic neuropathy and (ii) the NGC application for the optic nerve regeneration. The understanding of cellular and molecular concepts encompassed, ocular inflammation, extrinsic signaling and intrinsic signaling for axon growth, mobile zinc role, Ca2+ factor associated with the optic nerve, alternative therapies from nanotechnology based on the molecular information and finally the nanotechnological outlook encompassing applicable biomaterials and the use of NGC for regeneration. The challenges and future outlook regarding optic nerve regenerations are also discussed. Upon the many approaches used, the comprehensive role of the cellular and molecular mechanism may set grounds for the efficient application of the NGC for optic nerve regeneration.

神经损伤可以等同于严重的损害,标准的治疗方法,如使用自体移植物或手术,会带来并发症,并能缩短缓解时间。与视神经再生有关的机制似乎尚未完全揭示。由于视神经受到直接或间接的损伤而导致的视力丧失的普遍比率是惊人的。目前,神经引导导管(NGC)的使用在一定程度上是可靠的,特别是在啮齿动物和周围神经系统中,这是一个有希望的再生和功能恢复的基础,然而在视神经中,这种NGC功能似乎并不为人所知。NGC的应用不足和视神经的完整再生可能是由于对细胞和分子活动的信息有限。本文就外伤性视神经病变的细胞和分子活性及NGC在视神经再生中的应用作一综述。对细胞和分子概念的理解包括,眼部炎症,轴突生长的外在信号和内在信号,移动锌的作用,与视神经相关的Ca2+因子,基于分子信息的纳米技术替代疗法,最后是纳米技术的前景,包括适用的生物材料和使用NGC进行再生。并对视神经再生面临的挑战和前景进行了展望。基于多种方法,细胞和分子机制的综合作用可能为NGC在视神经再生中的有效应用奠定基础。
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引用次数: 0
Homologous-adhering/targeting cell membrane- and cell-mediated delivery systems: a cancer-catch-cancer strategy in cancer therapy. 同源粘附/靶向细胞膜和细胞介导的传递系统:癌症治疗中的癌症捕获策略。
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-11-21 eCollection Date: 2025-01-01 DOI: 10.1093/rb/rbae135
Chenguang Liu, Jingjie Gao, Yuying Cheng, Shanshan Zhang, Caiyun Fu

Low tumor enrichment remains a serious and urgent problem for drug delivery in cancer therapy. Accurate targeting of tumor sites is still a critical aim in cancer therapy. Though there have been a variety of delivery strategies to improve the tumor targeting and enrichment, biological barriers still cause most delivered guests to fail or be excreted before they work. Recently, cell membrane-based systems have attracted a huge amount of attention due to their advantages such as easy access, good biocompatibility and immune escape, which contribute to their biomimetic structures and specific surface proteins. Furthermore, cancer cell membrane-based delivery systems are referred to as homologous-targeting function in which they exhibit significantly high adhesion and internalization to homologous-type tumor sites or cells even though the exact mechanism is not entirely revealed. Here, we summarize the sources and characterizations of cancer cell membrane systems, including reconstructed single or hybrid membrane-based nano-/microcarriers, as well as engineered cancer cells. Additionally, advanced applications of these cancer cell membrane systems in cancer therapy are categorized and summarized according to the components of membranes. The potential factors related to homologous targeting of cancer cell membrane-based systems are also discussed. By discussing the applications, challenges and opportunities, we expect the cancer cell membrane-based homologous-targeting systems to have a far-reaching development in preclinic or clinics.

肿瘤低富集仍然是肿瘤治疗中亟待解决的问题。准确定位肿瘤部位仍然是癌症治疗的关键目标。尽管已有多种递送策略来提高肿瘤靶向性和富集性,但生物屏障仍然导致大多数递送的客体在起作用之前失败或被排出体外。近年来,基于细胞膜的系统因其易于接近、生物相容性好、免疫逃逸等优点,以及其仿生结构和特异性表面蛋白而受到广泛关注。此外,基于癌细胞膜的递送系统被称为同源靶向功能,尽管其确切机制尚未完全揭示,但它们对同源型肿瘤部位或细胞表现出显著的高粘附和内化。在这里,我们总结了癌细胞膜系统的来源和特征,包括重建的单一或杂交膜基纳米/微载体,以及工程癌细胞。此外,根据膜的组成对这些肿瘤细胞膜系统在癌症治疗中的最新应用进行了分类和总结。本文还讨论了与肿瘤细胞膜系统同源靶向相关的潜在因素。通过对其应用、挑战和机遇的探讨,我们期待基于肿瘤细胞膜的同源靶向系统在临床前或临床中有深远的发展。
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引用次数: 0
Correction to: Nanocarrier of Pin1 inhibitor based on supercritical fluid technology inhibits cancer metastasis by blocking multiple signaling pathways. 更正为基于超临界流体技术的 Pin1 抑制剂纳米载体通过阻断多种信号通路抑制癌症转移。
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-11-19 eCollection Date: 2024-01-01 DOI: 10.1093/rb/rbae132

[This corrects the article DOI: 10.1093/rb/rbad014.].

[此处更正了文章 DOI:10.1093/rb/rbad014]。
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引用次数: 0
Mimicking osteoid 3D porous dense microfiber silk fibroin embedded poly(vinyl alcohol) scaffold for alveolar ridge preservation. 三维多孔致密超纤维丝素嵌入聚乙烯醇支架用于牙槽嵴保存。
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-11-13 eCollection Date: 2025-01-01 DOI: 10.1093/rb/rbae130
Supaporn Sangkert, Perumal Ramesh Kannan, Jirut Meesane, Kanokporn Santavalimp, Jutharat Phongthanawarakun, Walaiporn Promkaew, Wachiratan Anupan, Nuttawut Thuaksuban

Alveolar ridge loss presents difficulties for implant placement and stability. To address this, alveolar ridge preservation (ARP) is required to maintain bone and avoid the need for ridge augmentation using socket grafting. In this study, a scaffold for ARP was created by fabricating a 3D porous dense microfiber silk fibroin (mSF) embedded in poly(vinyl alcohol) (PVA), which mimics the osteoid template. The research utilized a freeze-thawing technique to create a mimicked osteoid 3D porous scaffold by incorporating different amounts of mSF into the PVA, namely, 1%, 3%, 5% and 7%. Subsequently, a 3D profilometer machine and a scanning electron microscope were employed to examine the morphology and size of the mSF and the mimicked osteoid 3D porous scaffold in all groups. Thermal characteristics and crystalline structure were analyzed before assessing the water contact angle, swelling behavior, degradation and mechanical properties. The experiment evaluated the biological performance of the mimicked osteoid 3D porous scaffold by examining the efficacy of osteoblast cell adhesion, proliferation, viability, protein synthesis, alkaline phosphatase (ALP) activity and calcium synthesis. Finally, the ability of osteoblast cells to regulate the osteoid matrix deposition on the osteoid 3D porous scaffold was assessed by mimicking the dynamic bone environment using rat mesenchymal stem cells. The findings suggest that incorporating mSF into PVA enhances the interconnective pore size, crystalline structure and thermal behavior of the mimicked osteoid 3D porous scaffold. The hydrophilicity of PVA decreased with an increase in the proportion of mSF, while a higher proportion of mSF resulted in increased swelling and mechanical characteristics. Incorporating a greater proportion of mSF, specifically 5% and 7%, led to a reduced rate of degradation. The addition of 5% mSF to the PVA 3D porous scaffold resulted in remarkable biological properties and excellent osteoconductive activity.

牙槽嵴缺失给种植体的放置和稳定性带来困难。为了解决这个问题,需要进行牙槽嵴保存(ARP)来维持骨骼,避免使用窝骨移植来增加牙槽嵴。在这项研究中,通过制造嵌入聚乙烯醇(PVA)的三维多孔致密微纤维丝素(mSF),模拟类骨模板,创建了ARP支架。本研究利用冻融技术,将不同量的mSF掺入PVA中,分别为1%、3%、5%和7%,制备了模拟类骨3D多孔支架。随后,采用三维轮廓仪和扫描电镜检测各组mSF和模拟骨样三维多孔支架的形态和大小。分析了热特性和晶体结构,然后评估了水接触角、膨胀行为、降解和力学性能。本实验通过检测成骨细胞的粘附、增殖、活力、蛋白质合成、碱性磷酸酶(ALP)活性和钙合成等指标来评价模拟类骨3D多孔支架的生物学性能。最后,利用大鼠间充质干细胞模拟动态骨环境,评估成骨细胞调节类骨基质在类骨3D多孔支架上沉积的能力。研究结果表明,将mSF加入到PVA中可以增强模拟类骨3D多孔支架的相互连接孔径、晶体结构和热行为。随着mSF比例的增加,PVA的亲水性降低,而mSF比例的增加导致膨胀和力学特性的增加。加入更大比例的mSF,特别是5%和7%,导致降解率降低。在PVA三维多孔支架中添加5%的mSF,获得了显著的生物学性能和良好的骨传导活性。
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引用次数: 0
An immunoregulatory and metabolism-improving injectable hydrogel for cardiac repair after myocardial infarction. 一种用于心肌梗死后心脏修复的免疫调节和代谢改善注射水凝胶。
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-11-13 eCollection Date: 2025-01-01 DOI: 10.1093/rb/rbae131
Yage Sun, Xinrui Zhao, Qian Zhang, Rong Yang, Wenguang Liu

The hypoxia microenvironment post-myocardial infarction (MI) critically disturbs cellular metabolism and inflammation response, leading to scarce bioenergy supplying, prolonged inflammatory phase and high risk of cardiac fibrosis during cardiac restoration. Herein, an injectable hydrogel is prepared by Schiff base reaction between fructose-1,6-bisphosphate (FBP)-grafted carboxymethyl chitosan (CF) and oxidized dextran (OD), followed by loading fucoidan-coated baicalin (BA)-encapsulated zein nanoparticles (BFZ NPs), in which immunoregulatory and metabolism improving functions are integrally included. The grafted FBP serves to enhance glycolysis and provide more bioenergy for cardiomyocytes survival under hypoxia microenvironment, and elevating cellular antioxidant capacity via pentose phosphate pathway. OD with intrinsic anti-inflammatory effect can induce M2 polarization of macrophages to accelerate inflammatory elimination. While facing the possibility of endothelial-to-mesenchymal transition (EndoMT) caused by excessive expressed TGF-β1 secreted from M2 macrophages, BFZ NPs can target endothelia cells and intracellularly release BA to regulate the level of fatty acid oxidation, resulting in retained endothelial features and decreased risk of cardiac fibrosis. After being injecting the hydrogel into rats' infarcted cardiac, the 28-day-post surgical outcomes demonstrate its benign effects on restoring cardiac functions and attenuating adverse left ventricular remodeling. This study shows a promising measure for MI treatment with immunoregulating and metabolism regulation comprehensively.

心肌梗死(MI)后缺氧微环境严重扰乱细胞代谢和炎症反应,导致生物能量供应不足,炎症期延长,心脏恢复过程中心肌纤维化风险高。本研究以果糖-1,6-二磷酸(FBP)接枝羧甲基壳聚糖(CF)和氧化葡聚糖(OD)为原料,通过希夫碱反应制备了一种可注射的水凝胶,然后负载岩藻糖苷包被黄芩苷(BA)包被的玉米蛋白纳米颗粒(BFZ NPs),该纳米颗粒具有免疫调节和代谢改善功能。移植物FBP可促进糖酵解,为缺氧微环境下心肌细胞存活提供更多生物能量,并通过戊糖磷酸途径提高细胞抗氧化能力。具有内在抗炎作用的OD可诱导巨噬细胞M2极化,加速炎症消除。面对M2巨噬细胞分泌的TGF-β1过度表达导致的内皮-间质转化(EndoMT)的可能性,BFZ NPs可以靶向内皮细胞,在细胞内释放BA调节脂肪酸氧化水平,保留内皮特征,降低心肌纤维化风险。将水凝胶注射到梗死大鼠心脏,术后28天的结果显示其对恢复心功能和减轻不良左心室重构有良好的作用。本研究显示了免疫调节和代谢调节综合治疗心肌梗死的一种很有前景的方法。
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引用次数: 0
Advancements in nanohydroxyapatite: synthesis, biomedical applications and composite developments. 纳米羟基磷灰石的进展:合成、生物医学应用和复合材料的发展。
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-11-05 eCollection Date: 2025-01-01 DOI: 10.1093/rb/rbae129
Rui Zhao, Xiang Meng, Zixian Pan, Yongjia Li, Hui Qian, Xiangdong Zhu, Xiao Yang, Xingdong Zhang

Nanohydroxyapatite (nHA) is distinguished by its exceptional biocompatibility, bioactivity and biodegradability, qualities attributed to its similarity to the mineral component of human bone. This review discusses the synthesis techniques of nHA, highlighting how these methods shape its physicochemical attributes and, in turn, its utility in biomedical applications. The versatility of nHA is further enhanced by doping with biologically significant ions like magnesium or zinc, which can improve its bioactivity and confer therapeutic properties. Notably, nHA-based composites, incorporating metal, polymeric and bioceramic scaffolds, exhibit enhanced osteoconductivity and osteoinductivity. In orthopedic field, nHA and its composites serve effectively as bone graft substitutes, showing exceptional osteointegration and vascularization capabilities. In dentistry, these materials contribute to enamel remineralization, mitigate tooth sensitivity and are employed in surface modification of dental implants. For cancer therapy, nHA composites offer a promising strategy to inhibit tumor growth while sparing healthy tissues. Furthermore, nHA-based composites are emerging as sophisticated platforms with high surface ratio for the delivery of drugs and bioactive substances, gradually releasing therapeutic agents for progressive treatment benefits. Overall, this review delineates the synthesis, modifications and applications of nHA in various biomedical fields, shed light on the future advancements in biomaterials research.

纳米羟基磷灰石(nHA)以其独特的生物相容性、生物活性和生物可降解性而闻名,这些特性归因于其与人类骨骼矿物成分的相似性。本文讨论了nHA的合成技术,重点介绍了这些方法如何形成其物理化学属性,以及其在生物医学应用中的应用。通过掺入具有生物学意义的离子,如镁或锌,nHA的多功能性进一步增强,这可以提高其生物活性并赋予治疗特性。值得注意的是,含有金属、聚合物和生物陶瓷支架的nha基复合材料表现出增强的骨导电性和骨诱导性。在骨科领域,nHA及其复合材料有效地作为骨移植替代物,表现出优异的骨整合和血管化能力。在牙科中,这些材料有助于牙釉质再矿化,减轻牙齿敏感性,并用于牙种植体的表面修饰。对于癌症治疗,nHA复合材料提供了一个有希望的策略,以抑制肿瘤生长,同时保留健康组织。此外,nha基复合材料正在成为递送药物和生物活性物质的高表面比的复杂平台,逐渐释放治疗剂以获得渐进的治疗效果。本文综述了nHA的合成、修饰及其在生物医学领域的应用,并对未来生物材料的研究进展进行了展望。
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引用次数: 0
Conductive hydrogels: intelligent dressings for monitoring and healing chronic wounds. 导电水凝胶:用于监测和治疗慢性伤口的智能敷料。
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-11-01 eCollection Date: 2025-01-01 DOI: 10.1093/rb/rbae127
Ying Fang, Yiran Han, Lu Yang, Ranjith Kumar Kankala, Shibin Wang, Aizheng Chen, Chaoping Fu

Conductive hydrogels (CHs) represent a burgeoning class of intelligent wound dressings, providing innovative strategies for chronic wound repair and monitoring. Notably, CHs excel in promoting cell migration and proliferation, exhibit powerful antibacterial and anti-inflammatory properties, and enhance collagen deposition and angiogenesis. These capabilities, combined with real-time monitoring functions, play a pivotal role in accelerating collagen synthesis, angiogenesis and continuous wound surveillance. This review delves into the preparation, mechanisms and applications of CHs in wound management, highlighting their diverse and significant advantages. It emphasizes the effectiveness of CHs in treating various chronic wounds, such as diabetic ulcers, infected wounds, temperature-related injuries and athletic joint wounds. Additionally, it explores the diverse applications of multifunctional intelligent CHs in advanced wound care technologies, encompassing self-powered dressings, electrically-triggered drug delivery, comprehensive diagnostics and therapeutics and scar-free healing. Furthermore, the review highlights the challenges to their broader implementation, explores the future of intelligent wound dressings and discusses the transformative role of CHs in chronic wound management, particularly in the context of the anticipated integration of artificial intelligence (AI). Additionally, this review underscores the challenges hindering the widespread adoption of CHs, delves into the prospects of intelligent wound dressings and elucidates the transformative impact of CHs in managing chronic wounds, especially with the forthcoming integration of AI. This integration promises to facilitate predictive analytics and tailor personalized treatment plans, thereby further refining the healing process and elevating patient satisfaction. Addressing these challenges and harnessing emerging technologies, we postulate, will establish CHs as a cornerstone in revolutionizing chronic wound care, significantly improving patient outcomes.

导电水凝胶(CHs)代表了一种新兴的智能伤口敷料,为慢性伤口修复和监测提供了创新的策略。值得注意的是,CHs具有促进细胞迁移和增殖的作用,具有强大的抗菌和抗炎作用,促进胶原沉积和血管生成。这些功能与实时监测功能相结合,在加速胶原合成、血管生成和持续伤口监测方面发挥着关键作用。本文就其制备、作用机制及其在伤口管理中的应用进行综述,强调其多样性和显著的优势。它强调了CHs治疗各种慢性伤口的有效性,如糖尿病溃疡、感染伤口、温度相关损伤和运动关节伤口。此外,它还探讨了多功能智能CHs在先进伤口护理技术中的各种应用,包括自供电敷料,电触发药物输送,综合诊断和治疗以及无疤痕愈合。此外,该综述强调了其更广泛实施的挑战,探索了智能伤口敷料的未来,并讨论了CHs在慢性伤口管理中的变革性作用,特别是在人工智能(AI)预期整合的背景下。此外,本综述强调了阻碍人工智能广泛应用的挑战,深入研究了智能伤口敷料的前景,并阐明了人工智能在慢性伤口管理中的变革性影响,特别是即将到来的人工智能的整合。这种整合有望促进预测分析和定制个性化治疗计划,从而进一步改善治疗过程,提高患者满意度。我们认为,应对这些挑战并利用新兴技术,将使CHs成为革新慢性伤口护理的基石,显著改善患者的预后。
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引用次数: 0
Cell-microsphere based living microhybrids for osteogenesis regulating to boosting biomineralization. 基于细胞-微球的活体微混合体,用于调节成骨过程,促进生物矿化。
IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-10-29 eCollection Date: 2024-01-01 DOI: 10.1093/rb/rbae125
Zhaofan Hu, Yunyang Zhang, Jingjing Zhang, Ran Zheng, Yang Yang, Fei Kong, Haoran Li, Xinyan Yang, Shuhui Yang, Xiangdong Kong, Ruibo Zhao

Biomineralization-based cell-material living composites ex vivo showed great potential for living materials construction and cell regulation. However, cells in scaffolds with unconnected pores usually induce confined nutrient transfer and cell-cell communications, affecting the transformation of osteoblasts into osteocytes and the mineralization process. Herein, the osteoblast-materials living hybrids were constructed with porous PLLA microspheres using a rational design, in which cell-based living materials presented an improved osteoblast differentiation and mineralization model using rationally designed cell-microsphere composites. The results indicated that the microfluidic-based technique provided an efficient and highly controllable approach for producing on-demand PLLA microspheres with tiny pores (<5 μm), medium pores (5-15 μm) and large pores (>15 μm), as well as further drug delivery. Furthermore, the simvastatin (SIM)-loaded porous PLLA microsphere with ε-polylysine (ε-PL) modification was used for osteoblast (MC3T3-E1) implantation, achieving the cell-material living microhybrids, and the results demonstrated the ε-PL surface modification and SIM could improve osteoblast behavior regulation, including cell adhesion, proliferation, as well as the antibacterial effects. Both in vitro and in vivo results significantly demonstrated further cell proliferation, differentiation and cascade mineralization regulation. Then, the quantitative polymerase chain reaction or histological staining of typical markers, including collagen type I, alkaline phosphatase, runt-related transcription factor 2 and bone morphogenetic protein 2, as well as the calcium mineral deposition staining in situ, reconfirmed the transformation of osteoblasts into osteocytes. These achievements revealed a promising boost in osteogenesis toward mineralization at the microtissue level by cell-microsphere integration, suggesting an alternative strategy for materials-based ex vivo tissue construction and cell regulation, further demonstrating excellent application prospects in the field of biomineralization-based tissue regeneration.

基于生物矿化的体外细胞-材料活复合材料在活材料构建和细胞调控方面显示出巨大潜力。然而,未连通孔隙的支架中的细胞通常会导致营养物质传递和细胞间通讯受限,影响成骨细胞向骨细胞的转化和矿化过程。本文利用合理设计的细胞-微球复合材料,用多孔聚乳酸微球构建了成骨细胞-材料活体混合体,其中基于细胞的活体材料展示了一种改进的成骨细胞分化和矿化模型。结果表明,基于微流体的技术为按需生产具有微孔(15 μm)的聚乳酸微球以及进一步的药物输送提供了一种高效且高度可控的方法。结果表明ε-PL表面修饰和SIM能改善成骨细胞的行为调控,包括细胞粘附、增殖和抗菌作用。体外和体内研究结果均显著表明,细胞增殖、分化和级联矿化调控能力得到进一步提高。然后,典型标志物的定量聚合酶链反应或组织学染色,包括胶原 I 型、碱性磷酸酶、runt 相关转录因子 2 和骨形态发生蛋白 2,以及钙矿物质原位沉积染色,再次证实了成骨细胞向骨细胞的转化。这些成果揭示了通过细胞-微球整合在微组织水平促进成骨向矿化方向发展的前景,为基于材料的体外组织构建和细胞调控提供了另一种策略,进一步展示了在基于生物矿化的组织再生领域的良好应用前景。
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Regenerative Biomaterials
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