Objective: This study aimed to evaluate the structural reliability and validity of generalized anxiety disorder 7-item (GAD-7) scale in early pregnant women. Methods: In this cross-sectional study, 30,823 patients in early pregnancy registered in the Obstetrics and Gynecology Hospital of Fudan University completed the GAD-7 scale and patient health questionnaire-9 item (PHQ-9). The discriminative ability, reliability, construct validity, and criterion validity were assessed to evaluate the psychometric properties and factor structures. Items with a discrimination parameter (α) of <0.65, factor loading of <0.30, or cross loading of >0.40 in two or more factors simultaneously were deleted from the scale. Results: All GAD-7 scale items exhibited a high discrimination power. The reliability of the GAD-7 scale was good (Cronbach’s alpha coefficient = 0.891). Exploratory factor analysis extracted one factor with eigenvalues of greater than 1.0, which explained 61.930% of the common variance. Confirmatory factor analysis confirmed that the one-factor structure fitted the data well. The correlation coefficient with the PHQ-9 was 0.639. Conclusion: The Chinese version of the GAD-7 scale can be used as a screening tool for early pregnant women. It performs well in terms of discriminative ability, reliability, construct validity, and criterion validity. Pregnant women who screen positive may require more attention and investigation to confirm the presence of generalized anxiety disorder.
{"title":"Reliability and validity of generalized anxiety disorder 7-item scale in early pregnant women","authors":"Li-li Gong, Xiaodi Xie, Si-Tong Liu, Weihong Hu, Yu-Jie Niu, Yi Sun, Jun-sheng Liu, X. Xia","doi":"10.1097/RD9.0000000000000046","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000046","url":null,"abstract":"Objective: This study aimed to evaluate the structural reliability and validity of generalized anxiety disorder 7-item (GAD-7) scale in early pregnant women. Methods: In this cross-sectional study, 30,823 patients in early pregnancy registered in the Obstetrics and Gynecology Hospital of Fudan University completed the GAD-7 scale and patient health questionnaire-9 item (PHQ-9). The discriminative ability, reliability, construct validity, and criterion validity were assessed to evaluate the psychometric properties and factor structures. Items with a discrimination parameter (α) of <0.65, factor loading of <0.30, or cross loading of >0.40 in two or more factors simultaneously were deleted from the scale. Results: All GAD-7 scale items exhibited a high discrimination power. The reliability of the GAD-7 scale was good (Cronbach’s alpha coefficient = 0.891). Exploratory factor analysis extracted one factor with eigenvalues of greater than 1.0, which explained 61.930% of the common variance. Confirmatory factor analysis confirmed that the one-factor structure fitted the data well. The correlation coefficient with the PHQ-9 was 0.639. Conclusion: The Chinese version of the GAD-7 scale can be used as a screening tool for early pregnant women. It performs well in terms of discriminative ability, reliability, construct validity, and criterion validity. Pregnant women who screen positive may require more attention and investigation to confirm the presence of generalized anxiety disorder.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"6 1","pages":"249 - 253"},"PeriodicalIF":0.8,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43270023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.1097/rd9.0000000000000041
I. L. Puppo, Z. Tonyan, A. Saifitdinova, J. A. Loginova, A. Kinunen, A. N. Panina, Julia R. Pastuhova, O. A. Leonteva, O. Chiryaeva, Olga V. Маlysheva, O. Glotov, I. V. Poliakova, A. Tikhonov, T. Vavilova, I. Menshikova, Elvira V. Isakova, A. Glotov, Natalya K. Bichevaya
{"title":"Evaluating Chromosomal Segregation in a Family Where Both Spouses Carry an Autosomal Translocation","authors":"I. L. Puppo, Z. Tonyan, A. Saifitdinova, J. A. Loginova, A. Kinunen, A. N. Panina, Julia R. Pastuhova, O. A. Leonteva, O. Chiryaeva, Olga V. Маlysheva, O. Glotov, I. V. Poliakova, A. Tikhonov, T. Vavilova, I. Menshikova, Elvira V. Isakova, A. Glotov, Natalya K. Bichevaya","doi":"10.1097/rd9.0000000000000041","DOIUrl":"https://doi.org/10.1097/rd9.0000000000000041","url":null,"abstract":"","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49459362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.1097/RD9.0000000000000037
Dan Cao, Jue Wang, Yuan Yao, W. Yeung
Human endometrium undergoes dynamic shedding, regeneration, and differentiation, with remarkable changes in gene expression across the menstrual cycle. The development of a receptive endometrium within a particular time frame (window of implantation) is critical for successful embryo implantation. To understand the role of the endometrium in human fertility and regenerative biology, transcriptomic characterization of the endometrium has traditionally been pursued at the tissue bulk level using microarray and next-generation sequencing. Owing to the rapid development of single-cell RNA sequencing technology, researchers have uncovered heterogeneous molecular activities in individual cells masked by bulk analysis. In this review, we opted to mainly focus on single-cell analysis in endometrial research and introduce basic knowledge of single-cell RNA sequencing and the isolation of single cells from endometrial cells. We also discussed how single-cell approaches are used to understand the transformation and regeneration of the endometrium physiologically and uncover endometrial factors that contribute to uterine pathology.
{"title":"Single-cell analysis in endometrial research","authors":"Dan Cao, Jue Wang, Yuan Yao, W. Yeung","doi":"10.1097/RD9.0000000000000037","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000037","url":null,"abstract":"Human endometrium undergoes dynamic shedding, regeneration, and differentiation, with remarkable changes in gene expression across the menstrual cycle. The development of a receptive endometrium within a particular time frame (window of implantation) is critical for successful embryo implantation. To understand the role of the endometrium in human fertility and regenerative biology, transcriptomic characterization of the endometrium has traditionally been pursued at the tissue bulk level using microarray and next-generation sequencing. Owing to the rapid development of single-cell RNA sequencing technology, researchers have uncovered heterogeneous molecular activities in individual cells masked by bulk analysis. In this review, we opted to mainly focus on single-cell analysis in endometrial research and introduce basic knowledge of single-cell RNA sequencing and the isolation of single cells from endometrial cells. We also discussed how single-cell approaches are used to understand the transformation and regeneration of the endometrium physiologically and uncover endometrial factors that contribute to uterine pathology.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"6 1","pages":"197 - 207"},"PeriodicalIF":0.8,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47215743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.1097/rd9.0000000000000045
Pei-Ru Wei, Yi-Hua Yang
{"title":"Single-cell sequencing analysis of the maternal–fetal interface","authors":"Pei-Ru Wei, Yi-Hua Yang","doi":"10.1097/rd9.0000000000000045","DOIUrl":"https://doi.org/10.1097/rd9.0000000000000045","url":null,"abstract":"","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42831775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.1097/RD9.0000000000000043
Ruihuan Gu, Jing Fu, Naidong Ge, Zhichao Li, Bin Huang, Yan Xu, Yao-Yu Zou, Lu Li, Yi-juan Sun, Xiao-Xi Sun
Objective: The objective of this study is to study whether preimplantation genetic testing for aneuploidy (PGT-A) improves the clinical outcomes of infertile patients with repeated implantation failure (RIF) undergoing frozen-thawed embryo transfer. Methods: This is a retrospective analysis of clinical pregnancy, live birth, miscarriage rates, and obstetric and perinatal outcomes of women with RIF with or without PGT-A. Statistical analyses of categorical data were performed using propensity score matching (PSM), χ2 test, and Student’s t test. Results: We enrolled 466 patients with RIF, of which, 209 were in the RIF-PGT-A group. The rate of euploid blastocysts was significantly associated with age and day 5 or 6 blastocysts. There were significant differences between the RIF-PGT-A group and the RIF-non-PGT-A group across several parameters. After PSM, positive serum human chorionic gonadotropin (56.9% and 33.9%, P <0.01), clinical pregnancy (49.5% and 31.2%, P <0.01), live birth (43.1% and 25.7%, P <0.01), and fetal heart rates (50.0% and 29.8%, P <0.01) per transfer were significantly higher in the RIF-PGT-A group. Conclusion: Elective single-embryo transfer PGT-A can minimize the risk of obstetric and perinatal outcomes, especially fetal body weight, in women with RIF. Additionally, PGT-A can significantly improve pregnancy and live birth rates.
{"title":"Preimplantation genetic testing for aneuploidy improves clinical outcomes in patients with repeated implantation failure","authors":"Ruihuan Gu, Jing Fu, Naidong Ge, Zhichao Li, Bin Huang, Yan Xu, Yao-Yu Zou, Lu Li, Yi-juan Sun, Xiao-Xi Sun","doi":"10.1097/RD9.0000000000000043","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000043","url":null,"abstract":"Objective: The objective of this study is to study whether preimplantation genetic testing for aneuploidy (PGT-A) improves the clinical outcomes of infertile patients with repeated implantation failure (RIF) undergoing frozen-thawed embryo transfer. Methods: This is a retrospective analysis of clinical pregnancy, live birth, miscarriage rates, and obstetric and perinatal outcomes of women with RIF with or without PGT-A. Statistical analyses of categorical data were performed using propensity score matching (PSM), χ2 test, and Student’s t test. Results: We enrolled 466 patients with RIF, of which, 209 were in the RIF-PGT-A group. The rate of euploid blastocysts was significantly associated with age and day 5 or 6 blastocysts. There were significant differences between the RIF-PGT-A group and the RIF-non-PGT-A group across several parameters. After PSM, positive serum human chorionic gonadotropin (56.9% and 33.9%, P <0.01), clinical pregnancy (49.5% and 31.2%, P <0.01), live birth (43.1% and 25.7%, P <0.01), and fetal heart rates (50.0% and 29.8%, P <0.01) per transfer were significantly higher in the RIF-PGT-A group. Conclusion: Elective single-embryo transfer PGT-A can minimize the risk of obstetric and perinatal outcomes, especially fetal body weight, in women with RIF. Additionally, PGT-A can significantly improve pregnancy and live birth rates.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"7 1","pages":"12 - 19"},"PeriodicalIF":0.8,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46433089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.1097/rd9.0000000000000042
S. Tian, H. Ruan, W. Yeung, Yin-Lau Lee
{"title":"Blastocyst-like embryo surrogates from stem cells in implantation models","authors":"S. Tian, H. Ruan, W. Yeung, Yin-Lau Lee","doi":"10.1097/rd9.0000000000000042","DOIUrl":"https://doi.org/10.1097/rd9.0000000000000042","url":null,"abstract":"","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44118240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The purpose of this study was to evaluate the sperm motility and DNA integrity at different temperatures to analyze whether the sperms are suitable on the second day for insemination of in vitro matured oocytes by intra-cytoplasmic sperm injection (ICSI) following density gradient centrifugation (DGC) and swim-up (SU) procedures. Methods: Semen samples were collected from 30 outpatients who visited the Center for Reproductive Medicine for semen analyses. Following sperm selection by DGC and SU procedures, the liquified semen samples were divided into three groups and incubated at 4, 25, and 37°C, respectively. Following incubation for 24, 48, and 72 hours, the sperm motility and sperm DNA fragmentation index (DFI) were analyzed. Results: Following the combination of DGC and SU procedures, the sperm motility (91.8% ± 8.6% vs. 50.8% ± 13.1%) and DFI (5.1% ± 7.9% vs. 13.0% ± 11.6%) were significantly improved (P <0.01) compared to those without any treatment. The sperm motility of the 3 groups significantly declined (P <0.05) post-incubation compared to that of the groups prior incubation. However, sperm motility significantly increased (76.9% ± 10.4%) (P <0.05) at 25°C compared to that of the other 2 groups (53.5% ± 11.0% and 47.6% ± 10.2%). Sperm DFI significantly increased (P <0.05) at 37°C following incubation for 24 and 72 hours in comparison to that of the other 2 groups. However, the sperm DFI did not significantly increase when the sperm samples were incubated at 4 (5.7% ± 5.9%) and 25°C (6.8% ± 5.6%) for 24 hours compared to that before incubation (5.1% ± 7.9%). Conclusions: These results indicate that the sperm quality, in terms of motility and DFI, can be efficiently improved by DGC in combination with SU. Following which, the sperm samples can be incubated at 25°C and be used on the second day for insemination of in vitro matured oocytes by ICSI.
{"title":"Changes in human sperm motility and DNA fragmentation index after incubation at different temperatures following density gradient centrifugation and swim-up procedures","authors":"Yan-Nan Yang, Ling Wang, Yubao Liu, Yun-jing Xue, Chen-Chen Liu, Fei Shi, Xue Dai, R. Chian","doi":"10.1097/RD9.0000000000000039","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000039","url":null,"abstract":"Objective: The purpose of this study was to evaluate the sperm motility and DNA integrity at different temperatures to analyze whether the sperms are suitable on the second day for insemination of in vitro matured oocytes by intra-cytoplasmic sperm injection (ICSI) following density gradient centrifugation (DGC) and swim-up (SU) procedures. Methods: Semen samples were collected from 30 outpatients who visited the Center for Reproductive Medicine for semen analyses. Following sperm selection by DGC and SU procedures, the liquified semen samples were divided into three groups and incubated at 4, 25, and 37°C, respectively. Following incubation for 24, 48, and 72 hours, the sperm motility and sperm DNA fragmentation index (DFI) were analyzed. Results: Following the combination of DGC and SU procedures, the sperm motility (91.8% ± 8.6% vs. 50.8% ± 13.1%) and DFI (5.1% ± 7.9% vs. 13.0% ± 11.6%) were significantly improved (P <0.01) compared to those without any treatment. The sperm motility of the 3 groups significantly declined (P <0.05) post-incubation compared to that of the groups prior incubation. However, sperm motility significantly increased (76.9% ± 10.4%) (P <0.05) at 25°C compared to that of the other 2 groups (53.5% ± 11.0% and 47.6% ± 10.2%). Sperm DFI significantly increased (P <0.05) at 37°C following incubation for 24 and 72 hours in comparison to that of the other 2 groups. However, the sperm DFI did not significantly increase when the sperm samples were incubated at 4 (5.7% ± 5.9%) and 25°C (6.8% ± 5.6%) for 24 hours compared to that before incubation (5.1% ± 7.9%). Conclusions: These results indicate that the sperm quality, in terms of motility and DFI, can be efficiently improved by DGC in combination with SU. Following which, the sperm samples can be incubated at 25°C and be used on the second day for insemination of in vitro matured oocytes by ICSI.","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"6 1","pages":"243 - 248"},"PeriodicalIF":0.8,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48151259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Organoids as a model to study the human endometrium","authors":"Jian-Lin Li, Leqian Lin, Jiangming Zhong, Xintong Li, Cheuk-Lun Lee, P. Chiu","doi":"10.1097/rd9.0000000000000040","DOIUrl":"https://doi.org/10.1097/rd9.0000000000000040","url":null,"abstract":"","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47648230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.1097/rd9.0000000000000036
Dajin Li
{"title":"Executive Editor-in-Chief’s introduction for This Special Issue","authors":"Dajin Li","doi":"10.1097/rd9.0000000000000036","DOIUrl":"https://doi.org/10.1097/rd9.0000000000000036","url":null,"abstract":"","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49068320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.1097/RD9.0000000000000015
Chen-Yang Huang, Hai-Xiang Sun, Jie Mei
The impact of coronavirus disease 2019 (COVID-19) on endometriosis (EM) is currently unclear. Here, we aimed to describe the potential influence of COVID-19 on the pathogenesis, clinical symptoms, and treatment of EM. The cytokine storm caused by COVID-19 may induce the occurrence and progression of EM, and immunosuppression of COVID-19 may help the ectopic endometrium escape from immune clearance. Consequently, the forced social isolation and the cancelation of non-emergency medical treatment during the COVID-19 pandemic aggravate anxiety and psychological pressure, which can aggravate the symptoms related to EM and delay routine medical services.
{"title":"Potential impact of COVID-19 pandemic on endometriosis.","authors":"Chen-Yang Huang, Hai-Xiang Sun, Jie Mei","doi":"10.1097/RD9.0000000000000015","DOIUrl":"https://doi.org/10.1097/RD9.0000000000000015","url":null,"abstract":"<p><p>The impact of coronavirus disease 2019 (COVID-19) on endometriosis (EM) is currently unclear. Here, we aimed to describe the potential influence of COVID-19 on the pathogenesis, clinical symptoms, and treatment of EM. The cytokine storm caused by COVID-19 may induce the occurrence and progression of EM, and immunosuppression of COVID-19 may help the ectopic endometrium escape from immune clearance. Consequently, the forced social isolation and the cancelation of non-emergency medical treatment during the COVID-19 pandemic aggravate anxiety and psychological pressure, which can aggravate the symptoms related to EM and delay routine medical services.</p>","PeriodicalId":20959,"journal":{"name":"Reproductive and Developmental Medicine","volume":"6 3","pages":"138-143"},"PeriodicalIF":0.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9a/75/rd9-6-138.PMC9924788.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10276669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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