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Feasibility of growing conventional confectionary sunflower hybrids in organic agriculture: preliminary results of organic trials 在有机农业中种植传统糖果向日葵杂交种的可行性:有机试验的初步结果
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/RATPOV56-19861
Brankica Babec, N. Hladni, S. Šeremešić, M. Jocković, Nemanja Ćuk, S. Gvozdenac, V. Miklič, B. Vojnov
As organic agriculture represents one of the most sustainable ways of agricultural land use, the key purpose of this research was to improve confectionary sunflower production in organic agriculture. The trial compared seed yield per plant of conventional confectionary sunflower hybrids in organic vs conventional production. Subsequently, 1000 seed weight, seed yield per plant and kernel ratio of confectionary hybrids treated with the following fertilizers used in organic production: fertilizer of animal origin (G), fertilizer based on amino acids and nitrogen (T) and microbiological fertilizer (N) were compared with untreated control (C). Fertilizers enhanced yield of sunflower compared to untreated control; the highest yield was recorded in combined of G+N and G+T, all in organic agriculture system. NS Gricko had the lowest seed yield per plant compared to the other hybrids that significantly increased when treated with G+N fertilizers; same results were obtained in treatments of NS Slatki, and NS Leviathan. Preliminary results of the study indicated that application of organic fertilizers can have a positive effect on yield of sunflower in organic production conditions, which indicates that further research is necessary.
由于有机农业是最具可持续性的农业土地利用方式之一,本研究的主要目的是提高有机农业中糖果向日葵的产量。本试验比较了有机生产和常规生产下常规糖果向日葵杂交种的单株种子产量。对有机生产中使用的动物源性肥料(G)、氨基酸和氮基肥料(T)和微生物肥料(N)处理的糖果杂交种的千粒重、单株种子产量和籽粒比与未处理对照(C)进行比较。在有机农业系统中,G+N和G+T的组合产量最高。G+N处理显著提高了其他杂交种单株种子产量,而Gricko单株种子产量最低;NS Slatki和NS Leviathan处理的结果相同。初步研究结果表明,在有机生产条件下,施用有机肥对向日葵产量有积极影响,这表明有必要进一步研究。
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引用次数: 1
Characterisation of Serbian durum wheat genotypes based on UPOV-defined characteristics 基于upov定义特征的塞尔维亚硬粒小麦基因型特征
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/ratpov56-22555
Verica Takač, S. Mikić, M. Mirosavljević, V. Momčilović, D. Trkulja, A. Kondić-Špika, L. Brbaklić
Estimation of the level of durum wheat germplasm genetic diversity is important for its classification and efficient use in breeding programmes. The aim of this study was to assess genetic diversity of durum wheat genotypes developed at the Institute of Field and Vegetable Crops in Novi Sad, Serbia, with 26 morphological characteristics based on the International Union for Protection of New Varieties of Plants guidelines. The Shannon diversity index was used as an indicator of morphological diversity and it ranged from 0.283 for glaucosity of lower side of the flag leaf blade and density of hairiness of uppermost node of the culm, to 0.950 for the ear colour, with the mean value of 0.616, indicating a medium to high level of morphological diversity. On average, the diversity was higher for traits relating to generative organs than for those associated with vegetative plant organs. The 21 morphological characteristics were sufficient to distinguish unique profiles of all durum wheat genotypes. The estimation of varietal diversity and identification of morphological characteristics with the highest discriminative power were done by multiple correspondence analysis. The traits that contributed the most to the distinction of genotypes were the ear colouration, length of beak of the lower glume, lower glume shape, ear length of awns at tip relative to ear length and colour of awns. Morphological characterization using the traits with the highest discriminative power could be a useful complementary method for durum wheat germplasm classification and diversity analysis.
硬粒小麦种质资源遗传多样性水平的评估对其分类和育种规划的有效利用具有重要意义。本研究的目的是根据国际植物新品种保护联盟指南,评估塞尔维亚诺维萨德大田和蔬菜作物研究所开发的具有26个形态特征的硬粒小麦基因型的遗传多样性。以Shannon多样性指数作为形态多样性的指标,其范围为旗叶下侧的白霜度和茎上节的毛密度为0.283,穗色为0.950,平均值为0.616,表明其形态多样性处于中高水平。平均而言,与生殖器官相关的性状多样性高于与营养器官相关的性状多样性。21个形态特征足以区分所有硬粒小麦基因型的独特特征。利用多重对应分析对品种多样性进行估计,并鉴定具有最高判别能力的形态特征。对基因型差异贡献最大的性状是穗色、下颖片喙长、下颖片形状、芒尖相对于穗长和芒色的穗长。利用鉴别力最高的性状进行形态鉴定,可作为硬粒小麦种质分类和多样性分析的有益补充方法。
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引用次数: 2
Vigna lanceolata in the fire-stick farming and the Australian Aboriginal culture 杉木中的火棒种植和澳大利亚土著文化
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/RATPOV56-19716
A. Castelli, A. Mikić
An amphicarpic and tuberous legume yam Vigna lanceolata Benth., endemic to Australia, has had an extraordinary significance in the peculiar type of farming and entire culture of the Aboriginal peoples for tens of millennia. Our review, welding plant and human sciences, offers a concise and informative presentation of the species, by elaborating its taxonomy and morphology, describing its beneficial nutritional and medicinal properties, exploring its ecogeography, with a remarkable population abundance in the northern areas of the continent, assessing its extensive spreading as a consequence of the past fire-use environmental changes and ways of its wilderness reconnaissance, harvest and food preparation, depicting its role in the Australian Aboriginal art, as one of the most sacred objects in the Dreamtime religion, presenting its vernacular names in 85 extinct and living Australian Aboriginal languages and concluding with opening new horizons of its research, through recently established biodiversity and breeding programmes.
一种两性结节状豆科山药。它是澳大利亚特有的一种植物,在土著居民独特的耕作方式和整个文化中具有非凡的意义,已有数万年的历史。通过阐述其分类和形态,描述其有益的营养和药用特性,探索其在大陆北部地区显着的种群丰富的生态地理,评估其因过去使用火的环境变化而广泛传播的结果,以及其野外侦察,收获和食物制备的方式,我们的回顾,结合植物和人文科学,提供了一个简明而翔实的物种介绍。描述了它在澳大利亚土著艺术中的作用,作为梦幻时代宗教中最神圣的物品之一,用85种已灭绝和现存的澳大利亚土著语言介绍了它的方言名称,并通过最近建立的生物多样性和育种计划,为其研究开辟了新的视野。
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引用次数: 0
Yield of biomass and essential oil of dill (Anethum graveolens L.) grown under irrigation 灌溉条件下莳萝的生物量和精油产量
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/RATPOV56-19792
V. Popović, L. Maksimović, D. Adamović, V. Sikora, V. Ugrenović, V. Filipović, K. Mačkić
Dill (Anethum graveolens L.) is a one-year herbaceous, spicy plant, which has aromatic smell and taste. One of its important ingredients is essential oil. The experiment showing the effect of irrigation on essential oil and biomass yield of dill was conducted in rainy 2016 and dry 2017 in two variants: control (without irrigation) and variant with irrigation. Favourable water balance of the soil was provided by applying drop-by-drop irrigation method. The average yield of fresh biomass was 8,252 kg ha-1 (10,194 kg ha-1 under irrigation and 6,310 kg ha-1 in control). Under irrigation, the yield of biomass was higher than the control variant by 78.59% in 2017 and 47.41% in 2016. Under irrigation, essential oil content and yield were higher than the control by 10.73% and 95.97% in 2017, respectively, and 3.93% and 54.06% in 2016, respectively.
莳萝(Anethum graveolens L.)是一年生草本植物,具有芳香的气味和味道。它的重要成分之一是精油。灌溉对莳萝精油和生物量产量影响的试验于2016年阴雨季节和2017年干旱季节进行,试验采用对照(不灌溉)和有灌溉的两种变异。采用滴灌方式,土壤水分平衡良好。新鲜生物量平均产量为8252 kg ha-1(灌溉条件下10194 kg ha-1,对照条件下6310 kg ha-1)。灌溉条件下,2017年和2016年的生物量产量分别比对照高78.59%和47.41%。灌溉条件下,2017年挥发油含量和产量分别比对照高10.73%和95.97%,2016年分别比对照高3.93%和54.06%。
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引用次数: 0
Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia 塞尔维亚一种新的芥菜病原菌——萝卜黄病毒的分子特征
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/ratpov56-23514
D. Milošević, M. Ignjatov, A. Marjanović-Jeromela, I. Stanković, Z. Nikolić, G. Tamindžić, B. Krstic
In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Sancevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.
2018年,我们采集了来自塞尔维亚Rimski Sancevi和Senta两个不同地区的58份芥菜样品,并采用tas - eltsa检测了芜菁黄病毒(TuYV)、花菜花叶病毒(CaMV)和芜菁花叶病毒(TuMV)的存在。42份检测标本血清学检测到TuYV,而CaMV和TuMV均为阴性。采用蚜虫传播试验,用2株自然感染的TuYV阳性芥菜苗接种5株试验植株。用特异引物TuYVorf0F/TuYVorf0R扩增780 bp的病毒片段,进行病毒种类鉴定。对分离株88Sal (MK144816)和98Bni (MK144817)的RTPCR产物进行测序,并与TuYV的GenBank序列进行比较。塞尔维亚分离株与波兰TuYV分离株(EU022489)的同源性最高。系统发育分析表明,来自塞尔维亚的TuYV分离株与从GenBank检索到的其他TuYV序列聚类。
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引用次数: 1
Molecular profiling of chickpea mutants isolated from EMS and gamma rays treatments em和γ射线处理分离鹰嘴豆突变体的分子谱分析
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/ratpov56-19959
S. Umavathi, L. Mullainathan
Conventional mutation breeding techniques have often been used to improve yield, disease and pest resistance in crop plants. In the present attempt, two well-known mutagenic agents ethyl methane sulphonate and EMS were employed in order to induce genetic variability variety of chick pea Co -4 obtained from Tamil Nadu Agricultural University. The seeds were treated with different concentrations of EMS (10-50 mM) and Gamma rays (20-60 kR). A total of 369 mutants which alter the height, leaf structure, colour of flower, seed, size of pod, duration of the plant were isolated and characterized. Among them, five true breeding mutants namely Early flowering mutant (EFM), Bold pod mutant (BPM), Bold seed mutant BSM), High yielding mutant (HYM) and High protein content mutant (HPC), which directly influenced the economic value of the crop were selected for further study. The results revealed a significant positive increase in the qualitative traits among the mutant lines. The RAPD profiling of isolated mutants revealed a total of 129 bands, among them 92 were polymorphic with an average of 70.99 percentage of polymorphism. Apart from 28 bands, all the bands seemed to be mutation. The presence of new bands and absence of existing bands might be the reason for quality improvement of chickpea. The RAPD is considered as an excellent marker to differentiate mutants from their parents, to assess genetic variation and phylogenetic relationship.
传统的突变育种技术经常被用来提高作物的产量和抗病虫害能力。以泰米尔纳德邦农业大学获得的鹰嘴豆Co -4为材料,采用两种著名诱变剂甲烷磺酸乙酯和EMS诱变。用不同浓度的EMS (10 ~ 50 mM)和γ射线(20 ~ 60 kR)处理种子。共分离和鉴定了369个突变体,这些突变体改变了植物的高度、叶结构、花的颜色、种子、荚果大小和持续时间。其中,选择直接影响作物经济价值的5个真育种突变体,即早花突变体(EFM)、果荚突变体(BPM)、果籽突变体(BSM)、高产突变体(HYM)和高蛋白含量突变体(HPC)进行进一步研究。结果表明,各突变系间的质量性状均有显著的正增加。分离突变体的RAPD图谱共发现129个条带,其中92个条带为多态,平均多态性率为70.99%。除28条条带外,其余条带均为突变。新条带的出现和现有条带的缺失可能是鹰嘴豆品质改善的原因。RAPD被认为是区分突变体与其亲本、评估遗传变异和系统发育关系的良好标记。
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引用次数: 0
Comparison of methods for germination testing of Cannabis sativa seed 大麻种子发芽试验方法的比较
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/ratpov56-21105
D. Jovičić, Z. Nikolić, V. Sikora, G. Tamindžić, Gordana Petrović, M. Ignjatov, D. Milošević
The most critical aspect of plant survival and reproduction is seed germination. Considering the specificity of the Cannabis sativa production, obtaining high-quality hemp seed is not an easy task. Germination testing is often the most reliable way of assessing viability, but the selection of appropriate methods is complex. This study aimed to evaluate the effect of different conditions (i.e. various methods of germination testing) on the successful process of hemp seed germination, achieving maximum germination potential. Our results showed that C. sativa seeds germinated well in different methods, such as between filter papers, top of filter papers and sand, but there were significant differences between the applied methods. This means that the hemp seeds may have different requirements for germination than conditions covered by the Rules recommended methods. Further, the results show that hemp seed may have certain residual dormancy that needs to be investigated in detail.
植物的生存和繁殖最关键的方面是种子发芽。考虑到大麻生产的特殊性,获得高质量的大麻种子并不是一件容易的事情。发芽试验通常是评估活力最可靠的方法,但选择合适的方法是复杂的。本研究旨在评估不同条件(即各种发芽测试方法)对大麻种子成功发芽过程的影响,以获得最大的发芽潜力。结果表明,不同处理方式(滤纸间、滤纸顶、砂层)对苜蓿种子的萌发效果均较好,但不同处理方式之间存在显著差异。这意味着大麻种子对发芽的要求可能不同于规则推荐方法所涵盖的条件。结果表明,麻籽可能存在一定的残留休眠,有待进一步研究。
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引用次数: 6
Studies on the perennial pea Vavilovia formosa (Fabaceae) of mountain Aknasar population (Gegham Highland) and under ex situ conditions in the Botanical Gardens of Armenia Gegham高原Aknasar山种群和亚美尼亚植物园迁地条件下多年生豌豆Vavilovia formosa(豆科)研究
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/RATPOV56-19833
J. Akopian, A. Ghukasyan, Z. Hovakimyan, L. Martirosyan, Gevorg Zaroyan
Wild perennial pea Vavilovia formosa is a relic and an endangered species of the upper alpine flora of Armenia, specialized to scree habitats. The populations of V. formosa in Armenia, as well as in other sites of the world, are in danger of extinction and need both in situ and ex situ complementary investigation and conservation. Present article contains information on V. formosa Aknasar mountain population studies. Data on natural conditions, flora composition, ecological and coenotic characteristic of the habitat and bio-ecological features of V. formosa are given. The present state of Vavilovia population of mountain Aknasar is satisfactory. The activation of seed reproduction along with vegetative propagation is observed. One of the natural threats leading to the change and reduction of Vavilovia population was estimated to be the overgrowing of mobile scree habitats with turf-forming grasses, which creates conditions for the mountain-meadow vegetation development. V. formosa ex situ conservation strategy is one of the possible ways of its adaptive capacity estimation to environment changes as well as for its saving and using in basic and applied researches. An introductory experiment on the cultivation of Vavilovia in the Yerevan and Sevan Botanical Gardens has started, and some data on bio-morphological and eco-physiological peculiarities under ex situ conditions were obtained.
野生多年生豌豆Vavilovia formosa是亚美尼亚高山植物区系的遗迹和濒危物种,专门用于寻找栖息地。在亚美尼亚和世界其他地区,福尔摩沙的种群都有灭绝的危险,需要就地和移地的补充调查和保护。这篇文章包含了关于福尔摩沙阿克纳萨尔山种群研究的信息。文中介绍了福尔摩沙的自然条件、植物区系组成、生境的生态和群落特征以及生物生态特征。瓦维罗维亚目前的人口状况是令人满意的。观察到种子繁殖的激活和无性繁殖。草皮草的过度生长为山地草甸植被的发展创造了条件,是导致瓦维罗维亚种群数量变化和减少的自然威胁之一。迁地保护策略是评估其对环境变化的适应能力,以及在基础研究和应用研究中对其进行保护和利用的可能途径之一。在埃里温和塞万植物园进行了瓦维罗维亚的引种试验,获得了一些在迁地条件下的生物形态和生态生理特性的数据。
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引用次数: 2
Variety selection in intensive alfalfa cutting management 紫花苜蓿精耕细作管理中的品种选择
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/RATPOV56-20528
D. Milić, S. Katanski, B. Milošević, D. Živanov
Alfalfa is an autotetraploid, perennial forage crop adapted to different environments and management systems. Trial with 12 alfalfa populations was established in spring of 2014. The research involved 9 commercial alfalfa varieties from IFVCNS, Serbia, and included 3 experimental populations in the final phase of breeding program. The main objectives of this paper were to examine the impact of variety selection on alfalfa yield and plant height in intensive cutting management in order to define harvesting strategies (using 6 and 5 cuts per season), with main goal to maximize profit on alfalfa farms in Serbia and Southeast Europe. Our study clearly demonstrates that there is genetic potential in some Serbian varieties (NS Sila, Nera, NS Jelena, and Banat VS) for use in intensive cutting systems with 6 cuts per year that can meet farmers' needs in conventional or organic type of alfalfa hay production.This research represents an initial guideline for the alfalfa variety selection in intensive cutting management systems in Serbia and Southeast Europe.
紫花苜蓿是一种适应不同环境和管理制度的多年生同源四倍体饲料作物。2014年春季建立了12个苜蓿群体的试验。该研究涉及来自塞尔维亚IFVCNS的9个商业苜蓿品种,并包括育种计划最后阶段的3个实验群体。本文的主要目的是研究在集约化采伐管理中,品种选择对苜蓿产量和株高的影响,以便确定采伐策略(每季使用6次和5次采伐),主要目标是使塞尔维亚和东南欧的苜蓿农场利润最大化。我们的研究清楚地表明,一些塞尔维亚品种(NS Sila、Nera、NS Jelena和Banat VS)具有遗传潜力,可用于每年6次的集约切割系统,以满足农民在传统或有机类型苜蓿干草生产中的需求。本研究为塞尔维亚和东南欧集约化采伐管理系统中苜蓿品种选择提供了初步指导。
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引用次数: 5
The effect of Pseudomonas chlororaphis subsp. aurantiaca strain Q16 able to inhibit Fusarium oxysporum growth on potato yield 绿假单胞菌亚种的作用。菌株Q16能抑制马铃薯尖孢镰刀菌的生长
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.5937/RATPOV56-20428
D. Poštić, D. Jošić, Z. Lepšanović, G. Aleksić, D. Latković, M. Starović
This study assesses the potential of antibiotics-producing Pseudomonas chlororaphis strains to increase potato yield and to inhibit the mycelial growth of phytopathogenic fungi Fusarium oxysporum (Fo) isolated from potato. P. chlororaphis subsp. aurantiaca strain Q16 (PchlQ16) caused the highest growth inhibition (67.07%) of FoA2 isolate in vitro. In field trials the effect of PchlQ16 was measured as the number of stems, number and weight of tubers and a total potato yield of the Rudolph potato variety. Application of P. chlororaphis and the number of treatments exhibited a significant effect on the yield. Two treatments of PchlQ16 increased the total yield of tubers from 4.9% to 33.05%, while four treatments from 9.3% to 92.35%, compared to the control. Based on our field results we can recommend a frequent application of PchlQ16 (4 times) during potato growth season. The results of our in vitro experiment support these findings as the bacterial strain suppressed growth of F. oxysporum. In this investigation PchlQ16 was confirmed as an effective growth promoting agent in potato production and can be highly effective in prevention of F. oxysporum infection.
本研究评估了产抗生素的绿绿假单胞菌菌株提高马铃薯产量和抑制马铃薯植物病原真菌尖孢镰刀菌(Fo)菌丝生长的潜力。青霉亚种aurantiaca菌株Q16 (PchlQ16)对FoA2分离物的体外生长抑制率最高(67.07%)。在田间试验中,PchlQ16对鲁道夫马铃薯品种的茎数、块茎数和块茎重量以及马铃薯总产量的影响进行了测定。施用青霉量和处理次数对产量有显著影响。PchlQ16 2个处理的块茎总产量较对照提高4.9% ~ 33.05%,4个处理的块茎总产量较对照提高9.3% ~ 92.35%。根据田间试验结果,建议在马铃薯生长季节频繁施用PchlQ16(4次)。我们的体外实验结果支持这些发现,因为该菌株抑制了尖孢镰刀菌的生长。本研究证实PchlQ16在马铃薯生产中是一种有效的促生长剂,对尖孢镰刀菌侵染有较好的预防作用。
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引用次数: 0
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