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Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog 芹菜中“茄茄候选植物原体”的分子特征:以福托格为例
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5937/ratpov58-33227
T. Popović, P. Mitrović, A. Kosovac
'Candidates Phytoplasma solani', known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during August 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for 'Ca. P. solani' presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stoll 1 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmpl. Combining molecular tools PCR/RFLP and sequencing reviled two 'Ca. P. solani' multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/ Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the 'Ca. P. solani' diversity on celery, but supplemented with genotyping of the vmpl gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of 'Ca. P. solani' affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.
“候选植物原体solani”,以其不起眼的名称stolbur Phytoplasma而闻名,是一种感染塞尔维亚许多作物的植物病原体。2020年8月,在伏伊伏丁那省诺维萨德郊区的富托格,观察到芹菜植株叶片明显变黄和褪绿,偶尔伴有组织坏死。对12株芹菜(有症状株8株,无症状株4株)进行了“茄蚜Ca. P. solani”分析。根据stoll 1基因检测结果,所有有症状的芹菜植株都感染了stoll植物原体,并对tuf、stamp和vmpl三个基因进行了多基因分子鉴定。结合分子PCR/RFLP和测序技术,在芹菜中发现了两个多位点基因型:tuf-b/Rqg31/V14和tuf-b/ Rpm35/V14,各有4个样本。所获得的菌株基因分型结果与前人关于芹菜‘Ca. P. solani’多样性的资料一致,但补充了vmpl基因的分型。Futog被评估地区stolbur植物原体暴发与塞尔维亚的tuf-b流行病学循环有关,该循环主要与芹菜田中存在的卷花杂草相关,可能是植物原体接种源。目测结果表明,10-15%的“茄蚜”病芹菜植株散布在整个样地,与塞尔维亚stolbur植物原体主要媒介C. arvensis(半翅目:Auchenorrhyncha)相关的cixiid planther Hyalesthes obsoletus在作物中的传播相对应。
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引用次数: 0
A study of correlations and path analyses of some traits in sunflower parental lines 向日葵亲本系部分性状的相关关系及通径分析
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5937/RATPOV58-26782
V. Radic, I. Balalić, G. Jaćimović, M. Krstić, M. Jocković, S. Jocić
The trial in this study was carried out in field conditions throughout three years from plots where seed production of sunflower parental lines was established. Eight genotypes were examined; namely RHA-UK, RHA-ST-59, RHA-SES-IMI, RHA-SNRF, RHA-E-ANN-65, RHA-RU-3, RHA-CDN, and RHA-N-M-1. All the examined genotypes are parental components of the best sunflower hybrids developed by the Institute of Field and Vegetable Crops, Novi Sad, Serbia. Relationships between seed yield and eight different traits in sunflower were studied - oil and protein content, seed germination, 1000-seed weight, head diameter, number of branches and leaves and plant height. The same analyses were carried out for comparison of oil content. A highly significant positive correlation was determined between plant height and number of branches; plant height and the number of leaves; plant height and head diameter; plant height and seed yield; number of branches and yield; the number of leaves and yield; head diameter and seed yield; number of leaves and number of branches; number of leaves and head diameter and between seed germination and oil content. Path analysis indicated highest significant direct effect of plant height on yield (0.439**). The number of branches had a significant positive direct effect on yield (0.260*). In the study of indirect effects on yield, thirteen significant effects were determined. The study of direct effects on oil content showed that the plant height and number of leaves diameter had high significant effects (0.800** and -0.847**). The existence of indirect significant effects on oil content was determined in nine cases.
本研究在向日葵亲本系种子生产建立的地块上进行了为期三年的田间试验。检测了8种基因型;即rhaa - uk、rhaa - st -59、rhaa - ses - imi、rhaa - snrf、rhaa - e - ann -65、rhaa - ru -3、rhaa - cdn和rhaa - n - m -1。所有检测的基因型都是塞尔维亚诺维萨德大田和蔬菜作物研究所培育的最好的向日葵杂交品种的亲本成分。研究了向日葵种子产量与籽粒含油量、蛋白质含量、种子发芽率、千粒重、穗粗、枝叶数、株高等8个性状的关系。对含油量的比较进行了同样的分析。株高与枝数呈极显著正相关;株高和叶数;株高和穗直径;株高和种子产量;枝数和产量;叶数和产量;穗直径和种子产量;叶数和枝数;叶数与头直径之间以及种子萌发与含油量之间。通径分析表明,株高对产量的直接影响最高(0.439**)。枝数对产量有显著的正直接影响(0.260*)。在对产量间接影响的研究中,确定了13个显著影响。对含油量的直接影响研究表明,株高和叶径对含油量的影响非常显著(分别为0.800**和-0.847**)。在9个案例中,确定了对含油量存在间接显著影响。
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引用次数: 3
In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.) 离体花药培养技术在小麦双单倍体生产中的应用
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5937/RATPOV58-29902
Osama Kanbar Zuhair, C. Lantos, J. Pauk
Acknowledgements: The first author is thankful to Stipendium Hungaricum Scholarship program of the Hungarian government for supporting this work. This research was funded by the Hungarian Academy of Sciences, grant number “János Bolyai Research Scholarship”; National Research, Development and Innovation Office, grant number “OTKA-K_16-K119835”, “GINOP-2.2.1-15-2016-00026”, “GINOP2.2.1-18-2018-00005”, “TUDFO/51757/2019-ITM” and “TKP2020-NKA-21”. The authors thank Elizabeth Búza for the grammatical corrections.
鸣谢:第一作者感谢匈牙利政府的匈牙利奖学金计划对这项工作的支持。本研究由匈牙利科学院资助,资助号“János博雅研究奖学金”;国家研究发展创新办公室,批准号“OTKA-K_16-K119835”、“GINOP-2.2.1-15-2016-00026”、“GINOP2.2.1-18-2018-00005”、“TUDFO/51757/2019-ITM”和“TKP2020-NKA-21”。作者感谢Elizabeth Búza的语法更正。
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引用次数: 5
Classification of rapeseed genotypes based on quantitative traits using multivariate techniques 基于数量性状的油菜基因型多变量分类
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5937/ratpov58-35477
M. Jankulovska, D. Rajković, D. Miladinović, S. Terzić, N. Grahovac, A. Kondić-Špika, A. Marjanović-Jeromela
The genetic variability of 13 rapeseed genotypes created in Serbia was assessed at the Institute of Field and Vegetable Crops, Novi Sad, in three consecutive years. The genotypes were evaluated and classified based on 10 quantitative traits (plant height, height to the first branch, stem width, number of lateral branches, number of leaves, number of pods, seed yield per plant, 1000 seed weight, oil content and protein content), using principal component analysis (PCA) and two-way cluster analysis. The first four PCs with eigenvalues >1 contributed to 83% of the total explained variance. The PC analysis revealed that plant height, height to the first branch, number of lateral branches and number of leaves contributed maximum to the total divergence of the material. The genotypes were classified in two main clusters: the first cluster comprised three genotypes, while the other genotypes belonged to the second cluster which can further be divided to two subclusters. One genotype was not classified in these two clusters. The selected genotypes that have desirable traits may be used in hybridization programs for improvement of seed yield and traits that contribute to seed yield most.
诺维萨德大田和蔬菜作物研究所连续三年评估了塞尔维亚创造的13种油菜籽基因型的遗传变异。利用主成分分析(PCA)和双向聚类分析,对10个数量性状(株高、首枝高、茎宽、侧枝数、叶片数、荚果数、单株产量、千粒重、含油量和蛋白质含量)进行基因型评价和分类。特征值为>1的前四个pc贡献了总解释方差的83%。PC分析表明,株高、第一枝高、侧枝数和叶片数对材料总发散度贡献最大。基因型主要分为两类,第一类包括3个基因型,其他基因型均属于第二类,第二类又分为两个亚类。1个基因型在这两个集群中未被分类。所选择的具有理想性状的基因型可用于杂交计划,以提高种子产量和对种子产量贡献最大的性状。
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引用次数: 0
Archaeoentomological assessment of weevil (Coleoptera, Bruchidae) infestation level of pea (Pisum sativum) at the Late Bronze Age settlement Hissar 青铜时代晚期希萨尔定居点豌豆象鼻虫(鞘翅目,帚螨科)侵害程度的考古昆虫学评价
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5937/RATPOV58-31204
A. Medović, A. Mikić
A find of 2572 charred seeds of pea (Pisum sativum L.) was detected at the Late Bronze Age tell settlement Hissar near Leskovac, in Serbia, belonging to the Brnjica cultural group, 14-10 cent. BC. Two types of pea seeds were observed: apparently healthy seeds and seeds damaged by the activity of a weevil (Coleoptera, Bruchidae). At least two-fifths of all finds have apparently been infested most probably by pea weevil (Bruchus pisorum L.), one of the most important pea pests worldwide, especially in medium-moist and dry climates, such as Southern Europe and Australia. A large amount of infested pea seeds indicates a developed pea production on small plots, strongly indicating that cultivating this ancient pulse crop must have been well-rooted in field conditions. Previous DNA analyses of charred pea placed the ancient Hissar pea at an intermediate position between extantly cultivated pea (P. sativum L. subsp. sativum var. sativum) and a wild, winter hardy, 'tall' pea (P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.). Based on an assumption of its late harvest time and combined with pea weevil life cycle stage in charred seeds, it was possible to estimate the season during which the seeds were carbonized, namely, the second half of July or the first days of August at the latest. Older, final weevil instars were predominant before seed carbonization. The pea infestation rate at Hissar is one of the highest noted among pulses in the Old World and the highest among peas, so far.
公元前14-10美分,在塞尔维亚莱斯科瓦茨附近的青铜时代晚期定居点Hissar发现了2572颗烧焦的豌豆种子(Pisum sativum L.),属于Brnjica文化群体。观察到两种类型的豌豆种子:一种是明显健康的种子,另一种是被象鼻虫(鞘翅目,Bruchidae)活动破坏的种子。至少有五分之二的发现很可能被豌豆象鼻虫(Bruchus pisorum L.)侵染,豌豆象鼻虫是世界上最重要的豌豆害虫之一,特别是在中等湿润和干燥的气候中,如南欧和澳大利亚。大量受感染的豌豆种子表明在小块土地上有发达的豌豆生产,强烈表明种植这种古老的脉冲作物必须在田间条件下扎根。先前对烧焦豌豆的DNA分析将古代毛豆置于现存栽培豌豆(P. sativum L. subsp.)之间的中间位置。sativum var. sativum)和一种野生的、冬季耐寒的“高”豌豆(P. sativum subsp.)。(史蒂芬·比伯)Asch。et Graebn)。根据其收获时间较晚的假设,结合豌豆象鼻虫在烧焦种子中的生命周期阶段,可以估计出种子炭化的季节,即7月下半月或最迟8月上旬。在种子碳化之前,较老的末象鼻虫占优势。到目前为止,希萨尔的豌豆侵染率是东半球豆类中最高的之一,也是豌豆中最高的。
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引用次数: 2
Chemical composition of hyssop cv. 'Domaći ljubičasti' essential oil and its antimicrobial activity 牛膝草的化学成分。'Domaći ljubi<e:1> asti'精油及其抑菌活性
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5937/RATPOV58-31751
M. Aćimović, A. Varga, M. Cvetković, Pezo Lato, B. Lončar, M. Ignjatov, T. Zeremski
Hyssop (Hyssopus officinalis L., Lamiaceae) is a perennial shrub or subshrub violet-blue flowers in verticillasters and spicy taste with a pungent flavour. Besides being used as a culinary herb for flavouring and food preservation, this plant is also an ornamental, bee attracting plant and a traditional remedy for respiratory diseases and digestive disturbances. Hyssop is an essential oil-bearing plant, and its essential oil (Hyssopi aetheroleum) is used in the pharmaceutical, perfume and cosmetics industries as well as in aromatherapy. The objective of this study was to determine the chemical composition of essential oil of hyssop CV. "Domaći ljubičasti", grown in Serbia, and investigate its antimicrobial activity against 16 bacteria, mainly pathogens in the food industry. A total of 61 compounds were detected in the hyssop essential oil. The bicyclic monoterpene ketones CIS-pinocamphone (43.8%) and trans-pinocamphone (18.3%) were the most abundant, comprising 62.1%, followed by b-pinene (6.3%) and pinocarvone (6.1%). Hyssop essential oil expressed antibacterial activity against: Staphylococcus aureus, Escherichia coli, Bacillus cereus, Proteus hauseri, Listeria monocytogenes, Rhodococcus equi, Listeria ivanovii, Salmonella Enteritidis, Enterococcus faecalis, Listeria innocua and Bacillus spizizenii. Hyssop essential oil did not express antibacterial activity against Pseudomonas aeruginosa, Salmonella Typhimurium, Klebsiella aerogenes and Staphylococcus epidermidis. Results of this study show that hyssop essential oil has potential for using as natural supplement for control of foodborne diseases of microbiological origin, as well as flavor compositions (herbaceous, camphor-like odour with warm and spicy undernotes), especially for meat products, sauces, soups and seasonings.
牛膝草(Hyssopus officinalis L.,牛膝草科)是一种多年生灌木或亚灌木,花呈紫罗兰蓝色,呈紫罗兰色,呈紫罗兰色,味辛辣。除了用作调味和食品保存的烹饪草本植物外,这种植物也是一种观赏植物,吸引蜜蜂的植物,也是治疗呼吸系统疾病和消化系统紊乱的传统药物。牛膝草是一种含油植物,它的精油(牛膝草)被用于制药、香水和化妆品行业以及芳香疗法。本研究的目的是测定牛膝草挥发油的化学成分。"Domaći ljubi asti",生长在塞尔维亚,并研究其对16种细菌的抗菌活性,主要是食品工业中的病原体。从牛膝草挥发油中共检出61种化合物。双环单萜酮类化合物以顺-匹诺曹酮(43.8%)和跨-匹诺曹酮(18.3%)含量最多,占62.1%,其次是b-蒎烯(6.3%)和匹诺曹酮(6.1%)。牛蹄草精油对金黄色葡萄球菌、大肠杆菌、蜡样芽孢杆菌、豪瑟变形杆菌、单核增生李斯特菌、马红球菌、伊万诺氏李斯特菌、肠炎沙门氏菌、粪肠球菌、无菌性李斯特菌和棘芽孢杆菌均有抑菌活性。牛膝草精油对铜绿假单胞菌、鼠伤寒沙门菌、产气克雷伯菌和表皮葡萄球菌均无抑菌活性。本研究结果表明,牛膝草精油有潜力作为控制微生物来源的食源性疾病的天然补充剂,以及风味成分(草本、樟脑样气味,带有温暖和辛辣的底味),特别是用于肉制品、酱汁、汤和调味料。
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引用次数: 3
Chamomile essential oil quality after postharvest separation treatments 采后分离处理对洋甘菊精油品质的影响
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.5937/ratpov58-33346
M. Aćimović, B. Lončar, Biljana Kiprovski, Jovana Stankovic-Jeremic, M. Todosijević, L. Pezo, Jelena Jeremić
Ecological conditions of the growing areas, growing practices as well as harvest and postharvest processing influence the yield and quality of chamomile. The aim of this research was to determine the influence of chamomile separation methods, as postharvest treatments, on the essential oil quality and content, with a view to improve current primary processing of this valuable medicinal plant. In order to explain the structure of laboratory data that would give deeper assessment of similarities among various samples of chamomile essential oil, PCA was employed. Tested results showed that separation of chamomile plant material, as postharvest and pre-drying treatment, had significant influence on the number of identified compounds in the chamomile essential oil. The highest content of individual essential oil compounds had chamomile flowers with short stems, especially a-bisabolol, chamazulene, Z-spiroether and E-b-famesene. In the essential oil obtained from chamomile flowers with long stems, content of a-bisabolol and chamazulene were significantly lower, while E-b-famesene and Z-spiroether contents were significantly higher. Furthermore, in the essential oil obtained from chamomile plant material without separation, the lowest content of abisabolol and Z-spiroether, and the highest content of E-b-farnesene were recorded. The correlation analysis was performed to investigate the likeness in the amounts of the active compounds of essential oil from differently processed chamomile samples. All these results indicate the importance of precise and controlled postharvest treatments, since it clearly affects the essential oil quality and content in the primary processing of this valuable medicinal plant.
种植区的生态条件、种植方式以及收获和采后加工影响洋甘菊的产量和品质。本研究的目的是确定洋甘菊分离方法作为采收后处理对其精油质量和含量的影响,以期改进目前这种珍贵药用植物的初级加工。为了解释实验室数据的结构,从而更深入地评估洋甘菊精油不同样品之间的相似性,采用主成分分析法。实验结果表明,分离洋甘菊植物材料,采后和预干燥处理对洋甘菊精油中鉴定化合物的数量有显著影响。其中,短茎洋甘菊挥发油中单体挥发油化合物含量最高,特别是a-双abolol、chamazulene、Z-spiroether和E-b-famesene。长茎洋甘菊精油中a-bisabolol和chamazulene含量较低,E-b-famesene和Z-spiroether含量较高。此外,未经分离的洋甘菊植物原料精油中,阿异abolol和z -螺醚含量最低,e -b-法尼烯含量最高。通过相关分析,探讨不同加工方法洋甘菊挥发油中活性成分含量的相似性。这些结果表明采后处理的精确和控制的重要性,因为采后处理明显影响到这种珍贵药用植物的初级加工中精油的质量和含量。
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引用次数: 3
Evaluation of respiratory activity of albic luvisol contaminated by zinc and nickel during spring rapeseed cultivation 春油菜种植过程中锌、镍污染的白质苯丙醇呼吸活性评价
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2020-01-01 DOI: 10.5937/ratpov57-27018
M. Samardzic, V. I. Andreeva, Z. Galic, J. Vasin
In almost all industrially developed regions of the Russian Federation, the soil is contaminated with heavy metals (HM) often in concentrations which exceed limits of safety. This contamination causes degradation of agricultural land, which underlines the importance of the complex continuous monitoring of the dynamics of on-going changes in urban ecosystems against the background of natural processes. Quantitative assessment of the environmental sustainability of the soil according to the biological indicators of its condition is of enormous importance, in particular the balance of microbial carbon in soils, which are under varying degrees of anthropogenic pressure. Experiments were carried out to determine the phytoremediation potential of the spring rapeseed plants on soil contaminated with heavy metals. The aim of the study was to assess the respiratory activity of albic luvisol at different levels of its contamination with zinc and nickel under the conditions of a growing experiment with spring rapeseed plants. The experimental data on the respiratory activity of soil artificially contaminated with zinc and nickel in the dose range of 400-800 and 30-60 mg kg-1 of soil respectively, showed that microbial activity had a strong positive correlation with the presence of spring rapeseed plants in the vessels and weak correlation on the presence of toxic elements in the soil. According to the respiratory activity of albic luvisol, it was found that in the first 13 days of vegetation, cultivation of spring rapeseed plants had a positive effect on the sustainability of soil microbiocenosis to complex pollution with zinc and nickel.
在俄罗斯联邦几乎所有工业发达地区,土壤都受到重金属污染,其浓度往往超过安全限度。这种污染导致农业用地退化,这强调了在自然过程背景下对城市生态系统持续变化动态进行复杂持续监测的重要性。根据土壤状况的生物指标对土壤的环境可持续性进行定量评价是非常重要的,特别是在不同程度的人为压力下土壤微生物碳的平衡。通过试验研究了油菜对重金属污染土壤的修复潜力。本研究的目的是在春菜籽植物生长试验的条件下,评估不同程度的锌和镍污染下的白脂陆维醇的呼吸活性。在400 ~ 800 mg kg-1和30 ~ 60 mg kg-1剂量范围内对土壤进行人工锌和镍污染后,土壤呼吸活性的实验数据表明,土壤微生物活性与容器内春菜籽植物的存在呈强正相关,与土壤中有毒元素的存在呈弱相关。根据白脂luvisol的呼吸活性,发现在植被生长的前13天,种植春油菜对土壤微生物群落对锌镍复合污染的可持续性有积极的影响。
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引用次数: 0
Potential application of triticale cultivar 'Odisej' for the production of cookies 小黑麦品种Odisej在饼干生产中的潜在应用
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2020-01-01 DOI: 10.5937/ratpov57-24126
M. Belović, A. Torbica, D. Škrobot, J. Tomić, I. Čabarkapa, D. Živančev, Slaviša Štatkić, Vladimir Aćin, K. Kukurová, Z. Ciesarová
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引用次数: 3
Effect of organic and conventional farming system and sowing date on yield, seed oil and protein content in rapeseed cultivars 有机与常规耕作制度及播期对油菜品种产量、籽油和蛋白质含量的影响
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2020-01-01 DOI: 10.5937/ratpov57-23744
S. Terzić, A. Marjanović-Jeromela, M. Zorić, V. Sikora, Z. Milovac, P. Mitrović, I. Balalić, V. Radic
Rapeseed is one of the major oil crops, grown in various agroecological conditions. Interest in organic rapeseed is rising, with increasing importance to breeders to determine the need for specific organic breeding programs. The objective of this study was to determine the adaptive value of rapeseed cultivars in organic farming environments. Five winter rapeseed cultivars were grown in conventional and organic plots, each with three sowing dates in four replications. The trials were organized using a randomized block design. The effect of cultivar and farming on emergence, percentage of harvested plants, yield, oil and protein content were investigated. Locally recommended agricultural practices were used to keep the fields free from weeds, insects and diseases. In organic field, weeds were removed mechanically while insects were treated using organic insecticide. The seed samples for analysis of oil and protein content were taken during harvest. Considering agricultural practices, it was found that rapeseed can be successfully grown in organic agriculture, but further improvements are needed to increase stability of production. Early sowing date provides enough time for rapeseed growth before the winter and good overwintering. The cultivars had higher oil content in the conventional farming, while there was no significant effect of farming system on protein content. For cultivar Slavica, higher yield was recorded in organic system, while cultivars Banacanka and Nena had high yield in both farming systems. The results suggest that the existing conventional breeding material can be used as a good starting point for further trait improvements in organic farming.
油菜籽是主要的油料作物之一,可在各种农业生态条件下种植。对有机油菜籽的兴趣正在上升,对育种者来说,确定特定有机育种计划的必要性越来越重要。本研究的目的是确定油菜籽品种在有机耕作环境中的适应价值。在常规和有机地块上种植5个冬季油菜籽品种,每个品种4个重复,3个播期。试验采用随机区组设计。研究了品种和耕作方式对出苗率、收获率、产量、油脂和蛋白质含量的影响。采用当地推荐的农业做法,使田地免受杂草、昆虫和疾病的侵害。在有机农田中,除草采用机械除草,除虫采用有机杀虫剂。用于分析油脂和蛋白质含量的种子样品是在收获期间采集的。结合农业实践,发现油菜籽可以在有机农业中成功种植,但需要进一步改进以增加生产的稳定性。早播期为油菜冬前生长和良好的越冬提供了充足的时间。常规耕作方式下品种含油量较高,而不同耕作方式对蛋白质含量无显著影响。品种Slavica在有机耕作系统中产量较高,而品种Banacanka和Nena在两种耕作系统中均有较高产量。结果表明,现有的常规育种材料可作为有机农业进一步性状改良的良好起点。
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引用次数: 0
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