Pub Date : 2020-12-15DOI: 10.18231/2320-1924.2018.0018
Hira Iqbal, H. Hussain, F. Zara, Mehrunnisa
The objective of this study is to evaluate the effect of educational intervention in improving hypertension?related adherence to medication and health?related quality of life. This study includes the effects of pharmacist interventions on cardiovascular problems, economics, medication issues, medicine adherence, self-prescription, hospitalized patients and discharged patients. The review study shows that pharmacist and physician co management in order to control blood pressure is significant at decreasing blood pressure and rate of blood pressure control is improved. The pharmacist can also improve medication adherence, quality of life and patient knowledge about diseases and medication and give satisfaction to patient. In addition, the use of drugs is reduced. This study emphasizes that intervention of pharmacist in medication management or therapy is health effective. As a member of health care team pharmacist can increase the number of health outcomes. Hypertension is an important risk factor contributes to present epidemic cardiovascular problems with the pharmacist intervention in controlling of blood pressure its risk is going to decrease. Keywords: Hypertension, Role of pharmacist, Essential hypertension, Cardio-vascular problems, Adherence.
{"title":"The effect of an educational intervention in improving hypertension?related adherence to medication and health?related quality of life","authors":"Hira Iqbal, H. Hussain, F. Zara, Mehrunnisa","doi":"10.18231/2320-1924.2018.0018","DOIUrl":"https://doi.org/10.18231/2320-1924.2018.0018","url":null,"abstract":"The objective of this study is to evaluate the effect of educational intervention in improving hypertension?related adherence to medication and health?related quality of life. This study includes the effects of pharmacist interventions on cardiovascular problems, economics, medication issues, medicine adherence, self-prescription, hospitalized patients and discharged patients. The review study shows that pharmacist and physician co management in order to control blood pressure is significant at decreasing blood pressure and rate of blood pressure control is improved. The pharmacist can also improve medication adherence, quality of life and patient knowledge about diseases and medication and give satisfaction to patient. In addition, the use of drugs is reduced. This study emphasizes that intervention of pharmacist in medication management or therapy is health effective. As a member of health care team pharmacist can increase the number of health outcomes. Hypertension is an important risk factor contributes to present epidemic cardiovascular problems with the pharmacist intervention in controlling of blood pressure its risk is going to decrease.\u0000\u0000Keywords: Hypertension, Role of pharmacist, Essential hypertension, Cardio-vascular problems, Adherence.","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82768677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-15DOI: 10.18231/2320-1924.2018.0017
Mohammad Abdul Rahmman Al-Maeni, Shaymaa Fouad Rasheed
A crucial area of research in nanotechnology is the formation of environmentally benign nanoparticles. Both unicellular and multicellular play an important role in synthesis nanoparticles through the production of inorganic materials either intracellularly or extracellularly. The agents (pigments, siderophores, cell extracted metabolites and reducing compounds) were used to prepare silver nanparticles with different sizes and shapes. The color variations (dark yellow, slightly dark yellow and golden yellow) arising from changes in the composition, size, and shape of nanoparticles, surrounding medium can be monitored using UV-visible spectrophotometer. These effects are due to the phenomena called surface plasmon resonance. The silver nanoparticles have Plasmon resonances ranged between (390, 383 and 365) nm which they are among the limitation of silver nanoparticles (360 – 420 nm). AFM analysis of Ag NP’s showed partially purified big triangular Ag NP having edge length around ~1. mm. Hexagonal particles on the background of a matrix made up of some molecules which may be metabolites products are found. Small spherical nanoparticles embedded in some kind of matrix indicate that this molecule acts as capping agent, which inhibits further growth of nanoparticles. Also ribbons like structures of width around 50 nm which are intertwined are the noble and rare structures which are synthesized by this method. MIC of silver Nanoparticles for. E coli, ranges between 80-90 µg/ml.., Serrtia, ranges between 50-60 µg/ml and Shagilla, ranges between 90-100 µg/ml. Keywords: Metabolites product of microorganisms, silver nanoparticles.
{"title":"Fascinating approach for using metabolites products of living microorganisms as reducing agents for preparing silver nanoparticles","authors":"Mohammad Abdul Rahmman Al-Maeni, Shaymaa Fouad Rasheed","doi":"10.18231/2320-1924.2018.0017","DOIUrl":"https://doi.org/10.18231/2320-1924.2018.0017","url":null,"abstract":"A crucial area of research in nanotechnology is the formation of environmentally benign nanoparticles. Both unicellular and multicellular play an important role in synthesis nanoparticles through the production of inorganic materials either intracellularly or extracellularly. The agents (pigments, siderophores, cell extracted metabolites and reducing compounds) were used to prepare silver nanparticles with different sizes and shapes. The color variations (dark yellow, slightly dark yellow and golden yellow) arising from changes in the composition, size, and shape of nanoparticles, surrounding medium can be monitored using UV-visible spectrophotometer. These effects are due to the phenomena called surface plasmon resonance. The silver nanoparticles have Plasmon resonances ranged between (390, 383 and 365) nm which they are among the limitation of silver nanoparticles (360 – 420 nm). AFM analysis of Ag NP’s showed partially purified big triangular Ag NP having edge length around ~1. mm. Hexagonal particles on the background of a matrix made up of some molecules which may be metabolites products are found. Small spherical nanoparticles embedded in some kind of matrix indicate that this molecule acts as capping agent, which inhibits further growth of nanoparticles. Also ribbons like structures of width around 50 nm which are intertwined are the noble and rare structures which are synthesized by this method. MIC of silver Nanoparticles for. E coli, ranges between 80-90 µg/ml.., Serrtia, ranges between 50-60 µg/ml and Shagilla, ranges between 90-100 µg/ml. \u0000 \u0000Keywords: Metabolites product of microorganisms, silver nanoparticles.","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"136 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80251911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-15DOI: 10.18231/j.jpbs.2020.001
Kavita M. Jaiswal, S. Dudhgaonkar, Latesh B. Raghute, Mahek S. Kewalramani, Lohit S. Vaishnao
Introduction: Chloroquine (CQ) and Hydroxychloroquine (HCQ) has received huge momentum as possible treatment for COVID-19. In India the drug is approved for prophylaxis of asymptomatic health workers treating suspected or confirmed COVID-19 cases, and asymptomatic household contacts of confirmed patients. The U.S. Food and Drug Administration (FDA) has issued Emergency Use Authorization (EUA) of HCQ to treat COVID-19. Materials and Methods: Here we present the database of trials with these compounds in COVID-19. Discussion: Early 15 registered clinical trials of CQ and HCQ in COVID -19 positive patients in China, showed that the drugs were superior to the control treatment in inhibiting the exacerbation of pneumonia, improving lung imaging findings, promoting a virus-negative conversion, and shortening the disease course. Some studies had no positive results including a multinational registry analysis of HCQ with or without a macrolide for treatment of COVID-19 published online in Lancet on 22nd May 2020.The study showed that there was decreased in-hospital survival and an increased frequency of ventricular arrhythmias when HCQ was used for treatment of COVID-19.In the light of this publication the WHO temporarily paused the HCQ arm of its ‘Solidarity’ global clinical trial on 26th May 2020 and trials with HCQ /CQ for COVID were also suspended. The Lancet paper was in controversy until finally on June 4th 2020 the article was retracted by authors. Conclusions: Until we get results of WHO fast track ‘Solidarity’ clinical trial and other randomized clinical trials repurposing these drugs remains questionable. Keywords: CQVID-19 pandemic, Corona ‘Solidarity’ trial, Severe acute respiratory syndrome
{"title":"Chloroquine and Hydroxychloroquine in SARS-Cov-2. Repurposing the old drugs against today’s deadly disease","authors":"Kavita M. Jaiswal, S. Dudhgaonkar, Latesh B. Raghute, Mahek S. Kewalramani, Lohit S. Vaishnao","doi":"10.18231/j.jpbs.2020.001","DOIUrl":"https://doi.org/10.18231/j.jpbs.2020.001","url":null,"abstract":"Introduction: Chloroquine (CQ) and Hydroxychloroquine (HCQ) has received huge momentum as possible treatment for COVID-19. In India the drug is approved for prophylaxis of asymptomatic health workers treating suspected or confirmed COVID-19 cases, and asymptomatic household contacts of confirmed patients. The U.S. Food and Drug Administration (FDA) has issued Emergency Use Authorization (EUA) of HCQ to treat COVID-19.\u0000Materials and Methods: Here we present the database of trials with these compounds in COVID-19.\u0000Discussion: Early 15 registered clinical trials of CQ and HCQ in COVID -19 positive patients in China, showed that the drugs were superior to the control treatment in inhibiting the exacerbation of pneumonia, improving lung imaging findings, promoting a virus-negative conversion, and shortening the disease course. Some studies had no positive results including a multinational registry analysis of HCQ with or without a macrolide for treatment of COVID-19 published online in Lancet on 22nd May 2020.The study showed that there was decreased in-hospital survival and an increased frequency of ventricular arrhythmias when HCQ was used for treatment of COVID-19.In the light of this publication the WHO temporarily paused the HCQ arm of its ‘Solidarity’ global clinical trial on 26th May 2020 and trials with HCQ /CQ for COVID were also suspended. The Lancet paper was in controversy until finally on June 4th 2020 the article was retracted by authors.\u0000Conclusions: Until we get results of WHO fast track ‘Solidarity’ clinical trial and other randomized clinical trials repurposing these drugs remains questionable.\u0000\u0000Keywords: CQVID-19 pandemic, Corona ‘Solidarity’ trial, Severe acute respiratory syndrome","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77873407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Commelina diffusa is a plant rich in antioxidant compounds, used in traditional medicine to treat many diseases. This study aimed to evaluate the hepatoprotective and antioxidant activities of aqueous and methanolic extracts of Commelina diffusa. Total phenolic and flavonoids content of extracts were determined. Hepatotoxicity was induced in rats by administration of gentamicin (80 mg/kg, i.p.) once daily for 10 days. Simultaneously, extracts were given at doses of 100 and 200 mg/kg, p.o. At the end of treatment, body weight of rats was measured; blood and liver tissues were collected for biochemical and histopathological analysis. The results showed that aqueous and methanolic extracts of C. diffusa contain significant amounts of total phenols and flavonoids. These extracts significantly decreased (pCommelina diffusa could protect against hepatotoxic effects of gentamicin. Keywords: Commelina diffusa, Gentamicin, Hepatoprotective activity, Antioxidant.
{"title":"Hepatoprotective and antioxidant effects of Commelina diffusa Burm extracts on gentamicin-induced liver damage in rats","authors":"Kamdem Djoko Serges, P. Laure, Nchouwet Moise Legentil, Kodjio Norbert, Kamanyi Albert, Wansi Ngnokam Sl","doi":"10.18231/j.jpbs.2020.004","DOIUrl":"https://doi.org/10.18231/j.jpbs.2020.004","url":null,"abstract":"Commelina diffusa is a plant rich in antioxidant compounds, used in traditional medicine to treat many diseases. This study aimed to evaluate the hepatoprotective and antioxidant activities of aqueous and methanolic extracts of Commelina diffusa. Total phenolic and flavonoids content of extracts were determined. Hepatotoxicity was induced in rats by administration of gentamicin (80 mg/kg, i.p.) once daily for 10 days. Simultaneously, extracts were given at doses of 100 and 200 mg/kg, p.o. At the end of treatment, body weight of rats was measured; blood and liver tissues were collected for biochemical and histopathological analysis. The results showed that aqueous and methanolic extracts of C. diffusa contain significant amounts of total phenols and flavonoids. These extracts significantly decreased (pCommelina diffusa could protect against hepatotoxic effects of gentamicin.\u0000\u0000Keywords: Commelina diffusa, Gentamicin, Hepatoprotective activity, Antioxidant.","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76386470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-15DOI: 10.18231/j.jpbs.2020.005
S. Chaudhry
Healthcare expenditure is becoming a significant challenge for the financial sustainability of national health systems even in high economies. HEOR (Health Economic Outcome Research) analytics plays an increasingly vital role in a drug’s approval and reimbursement with payers. In broad terms, health economists study the functioning of healthcare systems and health-affecting behaviors such as smoking, diabetes, and obesity.
{"title":"Quantifying Pharmacoeconomics","authors":"S. Chaudhry","doi":"10.18231/j.jpbs.2020.005","DOIUrl":"https://doi.org/10.18231/j.jpbs.2020.005","url":null,"abstract":"Healthcare expenditure is becoming a significant challenge for the financial sustainability of national health systems even in high economies. HEOR (Health Economic Outcome Research) analytics plays an increasingly vital role in a drug’s approval and reimbursement with payers. In broad terms, health economists study the functioning of healthcare systems and health-affecting behaviors such as smoking, diabetes, and obesity.","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86621885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-20DOI: 10.36478/rjbsci.2020.15.21
I. Maina, K. I. Onifade, B. Saidu, A. A. Abubakar, S. Habibullah, Abdullahi Ishaq Jaafar, Y. A. Yabo, Abdurahman Jibreen Hassan
: Medicinal plant such as Ziziphus Spina-Christi (ZSC) has been used for the treatment and prevention of several ailments in human and animals.It is recommended for the management of diseases in which free radical species are produced as a result of oxidative stress. However, there is lack of systematic study on the antioxidant and antityrosinase capacities of ZSCF from Nigeria. The present study quantifies the anti-oxidant and anti tyrosinaseability of the ZSCF grown in the Gwaski, Southern Borno state Nigeria. Antioxidant activity was assessed by using 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH), Superoxide-Radical Scavenging Activity (SRSA), Ferric Reducing/Antioxidant Power (FRAP assay). Overall the ZSCF showed strong antioxidant ability and high anti tyrosinase effect. There is variation in the oxidation power (DPPH) with 15.5% at the concentration of 7.8 μ g mL G 1 -88.4% at 1000 μ g mL G 1 and 22.5% at 7.8 μ g mL G 1 as well as 63.5% at 1000 μ g mL G 1 for FRAP assay. The percentage antityrosinase activities varied from 4.1% at 7.8 μ g mL G 1 -70.5% at 1000 μ g mL G 1 for plant crude extract. The observed antioxidantand anti tyrosinase ability of ZSCF may be due to abundant presence of phenolic contents and high electron donating ability to neutralize free radicals.
当前位置药用植物如紫皮草(ZSC)已被用于治疗和预防人类和动物的几种疾病。它被推荐用于管理因氧化应激而产生自由基的疾病。然而，对尼日利亚产ZSCF的抗氧化和抗酪氨酸酶能力缺乏系统的研究。本研究量化了生长在尼日利亚博尔诺州南部Gwaski的ZSCF的抗氧化剂和抗酪氨酸酶的能力。采用2,2 -二苯基-1-苯基酰肼(DPPH)、超氧自由基清除活性(SRSA)、铁还原/抗氧化能力(FRAP)测定抗氧化活性。总体而言，ZSCF具有较强的抗氧化能力和较高的抗酪氨酸酶作用。FRAP法的氧化能力(DPPH)在浓度为7.8 μ g mL g1时为15.5%，在浓度为1000 μ g mL g1时为88.4%，在浓度为7.8 μ g mL g1时为22.5%，在浓度为1000 μ g mL g1时为63.5%。植物粗提物抗酪氨酸酶活性在7.8 μ g mL g1时为4.1%，在1000 μ g mL g1时为70.5%。所观察到的抗氧化和抗酪氨酸酶能力可能是由于丰富的酚含量和高的给电子能力，以中和自由基。
{"title":"Anti-Oxidant and Anti Tyrosinase Effect of Zizyphus Spina-Christi Seed Extract","authors":"I. Maina, K. I. Onifade, B. Saidu, A. A. Abubakar, S. Habibullah, Abdullahi Ishaq Jaafar, Y. A. Yabo, Abdurahman Jibreen Hassan","doi":"10.36478/rjbsci.2020.15.21","DOIUrl":"https://doi.org/10.36478/rjbsci.2020.15.21","url":null,"abstract":": Medicinal plant such as Ziziphus Spina-Christi (ZSC) has been used for the treatment and prevention of several ailments in human and animals.It is recommended for the management of diseases in which free radical species are produced as a result of oxidative stress. However, there is lack of systematic study on the antioxidant and antityrosinase capacities of ZSCF from Nigeria. The present study quantifies the anti-oxidant and anti tyrosinaseability of the ZSCF grown in the Gwaski, Southern Borno state Nigeria. Antioxidant activity was assessed by using 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH), Superoxide-Radical Scavenging Activity (SRSA), Ferric Reducing/Antioxidant Power (FRAP assay). Overall the ZSCF showed strong antioxidant ability and high anti tyrosinase effect. There is variation in the oxidation power (DPPH) with 15.5% at the concentration of 7.8 μ g mL G 1 -88.4% at 1000 μ g mL G 1 and 22.5% at 7.8 μ g mL G 1 as well as 63.5% at 1000 μ g mL G 1 for FRAP assay. The percentage antityrosinase activities varied from 4.1% at 7.8 μ g mL G 1 -70.5% at 1000 μ g mL G 1 for plant crude extract. The observed antioxidantand anti tyrosinase ability of ZSCF may be due to abundant presence of phenolic contents and high electron donating ability to neutralize free radicals.","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79870019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-20DOI: 10.36478/rjbsci.2020.22.25
A. Rani, V. Ambikapathy, A. Panneerselvam
{"title":"Diversity of Endophytic Fungi from Leaves of Cenetlla asiatica L. and Melia azedarach L.","authors":"A. Rani, V. Ambikapathy, A. Panneerselvam","doi":"10.36478/rjbsci.2020.22.25","DOIUrl":"https://doi.org/10.36478/rjbsci.2020.22.25","url":null,"abstract":"","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"28 1","pages":"22-25"},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74383067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-20DOI: 10.36478/rjbsci.2020.26.32
M. Idris
Page No.: 26-32 Volume: 15, Issue 2, 2020 ISSN: 1815-8846 Research Journal of Biological Sciences Copy Right: Medwell Publications Abstract: The research was conducted to evaluate the performance of tomatoes varieties on growth, yield and quality through the application of partial rootzone drying. The study was conducted in green house at the Faculty of Agriculture, Universitas Islam Sumatera Utara (UISU) Medan in May 2014 until September 2014. The planting media used were straw compost mixed with top soil enriched with Urea, SP-36 and KCL fertilizer filled into polybeg up to 10 kg in weight. Observation variables are: the growing, yield and quality of tomato.
{"title":"The Effect of Applying Partial Root Zone Drying Technique on Growth, Result and Quality of Tomato","authors":"M. Idris","doi":"10.36478/rjbsci.2020.26.32","DOIUrl":"https://doi.org/10.36478/rjbsci.2020.26.32","url":null,"abstract":"Page No.: 26-32 Volume: 15, Issue 2, 2020 ISSN: 1815-8846 Research Journal of Biological Sciences Copy Right: Medwell Publications Abstract: The research was conducted to evaluate the performance of tomatoes varieties on growth, yield and quality through the application of partial rootzone drying. The study was conducted in green house at the Faculty of Agriculture, Universitas Islam Sumatera Utara (UISU) Medan in May 2014 until September 2014. The planting media used were straw compost mixed with top soil enriched with Urea, SP-36 and KCL fertilizer filled into polybeg up to 10 kg in weight. Observation variables are: the growing, yield and quality of tomato.","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88853683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-20DOI: 10.36478/rjbsci.2020.5.14
P. Asama, F. K. Channya
: Fungi such as Aspergillus niger (brasiliensis), Aspergillus flavus, Alterneriadianthocola, Curvularia lunata, Curvulari apellesecens, Fusarium oxysporum, Fusarium equiseti, Microphomina phaseolina, Rhizopus stolonifer, Penicillium digitatumare associated with heavy losses of seeds, fruits, grains, vegetables and other plant products in transit and storage rendering them unfit for human consumption. The research sought to identify fungal pathogens of groundnut rot in storage in Hong Local Government area of Adamawa State, Nigeria a major groundnut producing area. A survey was carried out using random sampling on incidence of groundnut rot in the seven districts of Hong local government area in the month of July 2016. Isolation and identification, frequency of occurrence, virulence, as well as effect of pathogens on germination of groundnut seeds and seedling growth were carried out. Incidence of rot occurred in all seven districts with the highest in Hong, Hildi and Gaya, pathogens associated with the rot were identified as follows; Aspergillus niger (brasilensis), Aspergilus flavus, Penicillium chrysogenum, Rhizopus stolonifer, Paecilomyces lilacinus, Pseudallescheria boydii, Cylindrocarpon lichenicola and Scedosaporium prolificans. The frequent occurring fungi were Aspergillus niger (brasilensis) and Aspergillus flavus while the most virulent was Aspergillus flavus. There was significant reduction in seed germination and seedling growth at 99.99% probability level for both the Valencia(kampala) and Peruvian(kwathrumthrum) from 33.33% for un-inoculated to 11.00% for inoculated.Proper storage practices to reduce groundnut rot as well as enhance seed viability are therefore suggested.
:在运输和储存过程中，与种子、水果、谷物、蔬菜和其他植物产品损失严重有关的真菌，包括黑曲霉、黄曲霉、黄曲霉、弯曲霉、尖孢镰刀菌、马尾镰刀菌、菜豆小霉、匍匐茎霉、数字青霉等，使它们不适合人类食用。本研究旨在鉴定尼日利亚主要花生产区阿达马瓦州Hong Local Government地区储藏中的花生腐病真菌病原体。采用随机抽样的方法，于2016年7月对香港7个区县的花生腐病发病率进行了调查。对病原菌的分离鉴定、发生频率、毒力以及对花生种子萌发和幼苗生长的影响进行了研究。所有7个县都发生了腐病，其中Hong、Hildi和Gaya的发病率最高，与腐病相关的病原体鉴定如下;黑曲霉(巴西曲霉)、黄曲霉、青霉、匍匐根霉、淡紫拟青霉、博伊地假杆菌、地衣圆柱菌和增菌绿霉。常见真菌为黑曲霉(brasilensis)和黄曲霉(Aspergillus flavus)，毒力最强的为黄曲霉(Aspergillus flavus)。瓦伦西亚(坎帕拉)和秘鲁(夸特鲁姆)的种子发芽率和幼苗生长均显著降低，概率为99.99%，由未接种的33.33%降至接种后的11.00%。因此，建议采取适当的贮藏措施，以减少花生腐烂，提高种子活力。
{"title":"Fungal Pathogens of Postharvest Rot of Groundnut (Arachys hypogaea L.) in Hong Local Government Area of Adamawa State Nigeria","authors":"P. Asama, F. K. Channya","doi":"10.36478/rjbsci.2020.5.14","DOIUrl":"https://doi.org/10.36478/rjbsci.2020.5.14","url":null,"abstract":": Fungi such as Aspergillus niger (brasiliensis), Aspergillus flavus, Alterneriadianthocola, Curvularia lunata, Curvulari apellesecens, Fusarium oxysporum, Fusarium equiseti, Microphomina phaseolina, Rhizopus stolonifer, Penicillium digitatumare associated with heavy losses of seeds, fruits, grains, vegetables and other plant products in transit and storage rendering them unfit for human consumption. The research sought to identify fungal pathogens of groundnut rot in storage in Hong Local Government area of Adamawa State, Nigeria a major groundnut producing area. A survey was carried out using random sampling on incidence of groundnut rot in the seven districts of Hong local government area in the month of July 2016. Isolation and identification, frequency of occurrence, virulence, as well as effect of pathogens on germination of groundnut seeds and seedling growth were carried out. Incidence of rot occurred in all seven districts with the highest in Hong, Hildi and Gaya, pathogens associated with the rot were identified as follows; Aspergillus niger (brasilensis), Aspergilus flavus, Penicillium chrysogenum, Rhizopus stolonifer, Paecilomyces lilacinus, Pseudallescheria boydii, Cylindrocarpon lichenicola and Scedosaporium prolificans. The frequent occurring fungi were Aspergillus niger (brasilensis) and Aspergillus flavus while the most virulent was Aspergillus flavus. There was significant reduction in seed germination and seedling growth at 99.99% probability level for both the Valencia(kampala) and Peruvian(kwathrumthrum) from 33.33% for un-inoculated to 11.00% for inoculated.Proper storage practices to reduce groundnut rot as well as enhance seed viability are therefore suggested.","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82162873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-20DOI: 10.36478/rjbsci.2020.33.39
F. Rezaei, Maryam Hassan Famian, H. Shafaei, J. S. Rad, L. Roshangar, A. Montaseri
: To investigate the probable effects of mummy on chondrocyte proliferation and expression of cartilage specific gene. Chondrocytes at the second passage were plated at density of 1×10 6 in monolayer or seeded onto PCL scaffolds. Cells in both conditions were divided into control (received medium) or treated with mummy at concentrations of 500 or 1000 µg mL G 1 for 72 h. The gene expression profile of collagen II, aggrecan, Cartilage Oligomeric Matrix Protein (COMP) and Sox-9 was evaluated using real-time RT-PCR. The ability of mummy to enhance the proliferation of chondrocytes was further evaluated using doubling time. Treatment of chondrocytes with 1000 µg mL G 1 of mummy resulted in a significant increase in expression of Sox-9, COMP and aggrecan genes in both monolayer and 3D culture conditions. Compared to the monolayer cultivated cells, expression of genes in seeded chondrocytes on PCL scaffolds was significantly higher. The highest proliferation rate of chondrocytes was found at a concentration of 1000 µg mL G 1 . The preliminary findings of this study revealed that mummy can be a promoting factor for chondrocyte proliferation and ECM synthesis. The use of mummy at 1000 µg mL G 1
目的:探讨木乃伊对软骨细胞增殖及软骨特异性基因表达的影响。将第二次传代的软骨细胞以1×10 6的密度单层或播种到PCL支架上。将两种情况下的细胞分为对照(接受培养基)或500或1000µg mL g1浓度的木乃伊处理72 h。使用实时RT-PCR评估II型胶原蛋白、聚集蛋白、软骨寡聚物基质蛋白(COMP)和Sox-9的基因表达谱。用倍增时间进一步评价木乃伊促进软骨细胞增殖的能力。用1000µg mL g1木乃伊处理软骨细胞，在单层和三维培养条件下，都能显著增加Sox-9、COMP和聚集蛋白基因的表达。与单层培养细胞相比，种子软骨细胞在PCL支架上的基因表达显著增加。在浓度为1000µg mL g1时，软骨细胞增殖率最高。本研究的初步结果显示木乃伊可能是软骨细胞增殖和ECM合成的促进因子。使用妈咪量为1000µg mL g1
{"title":"Mummy Enhances Chondrocyte Proliferation and the Expression of Extracellular Matrix Components in Monolayer and 3D Culture Conditions","authors":"F. Rezaei, Maryam Hassan Famian, H. Shafaei, J. S. Rad, L. Roshangar, A. Montaseri","doi":"10.36478/rjbsci.2020.33.39","DOIUrl":"https://doi.org/10.36478/rjbsci.2020.33.39","url":null,"abstract":": To investigate the probable effects of mummy on chondrocyte proliferation and expression of cartilage specific gene. Chondrocytes at the second passage were plated at density of 1×10 6 in monolayer or seeded onto PCL scaffolds. Cells in both conditions were divided into control (received medium) or treated with mummy at concentrations of 500 or 1000 µg mL G 1 for 72 h. The gene expression profile of collagen II, aggrecan, Cartilage Oligomeric Matrix Protein (COMP) and Sox-9 was evaluated using real-time RT-PCR. The ability of mummy to enhance the proliferation of chondrocytes was further evaluated using doubling time. Treatment of chondrocytes with 1000 µg mL G 1 of mummy resulted in a significant increase in expression of Sox-9, COMP and aggrecan genes in both monolayer and 3D culture conditions. Compared to the monolayer cultivated cells, expression of genes in seeded chondrocytes on PCL scaffolds was significantly higher. The highest proliferation rate of chondrocytes was found at a concentration of 1000 µg mL G 1 . The preliminary findings of this study revealed that mummy can be a promoting factor for chondrocyte proliferation and ECM synthesis. The use of mummy at 1000 µg mL G 1","PeriodicalId":21014,"journal":{"name":"Research journal of pharmaceutical, biological and chemical sciences","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84310313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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