Reproductive biology最新文献_第10页

Investigating the clinical significance of immune and thyroid biomarkers in women with breast cancer and Hashimoto's thyroiditis 探讨免疫和甲状腺生物标志物在乳腺癌和桥本甲状腺炎患者中的临床意义

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-06-01 Epub Date: 2025-04-12 DOI: 10.1016/j.repbio.2025.101011

Israa Khalaf Aneed , Noori Mohammed Luaibi , Sajid Nader Abdulqader

Breast cancer with Hashimoto’s thyroiditis (BC-HT) presents a unique immuno-thyroid interplay that remains poorly understood. This study investigates the relationships between thyroid function markers (TSH, T3, T4), immune markers (CD33, CD44), and thyroid autoantibodies (Anti-TPO, Anti-Tg) in BC-HT patients and healthy controls. Normality testing confirmed non-parametric data distribution, necessitating Mann-Whitney U tests for group comparisons. BC-HT patients exhibited significantly elevated TSH, CD33, Anti-TPO, and Anti-Tg levels, alongside reduced T3 and T4, compared to controls, indicating thyroid dysfunction. Spearman’s correlation analysis revealed strong negative correlations between TSH and T3/T4 in controls, which were lost in BC-HT, suggesting disruption of normal thyroid feedback mechanisms. Additionally, CD33 and CD44 correlations with thyroid hormones were evident in controls but absent in BC-HT, highlighting altered immune-thyroid interactions. ROC analysis demonstrated high diagnostic performance for TSH, Anti-Tg, and Anti-TPO, with sensitivities exceeding 0.75, whereas CD33 and CD44 showed limited diagnostic utility. These findings suggest a distinct immuno-thyroid dysregulation in BC-HT patients and highlight the potential of thyroid-specific markers for disease stratification. Future research should focus on longitudinal studies and mechanistic investigations to further delineate the role of immune markers in breast cancer pathophysiology within the context of thyroid autoimmunity.

乳腺癌合并桥本甲状腺炎（BC-HT）呈现出一种独特的免疫-甲状腺相互作用，目前尚不清楚。本研究探讨BC-HT患者和健康对照者甲状腺功能标志物（TSH、T3、T4）、免疫标志物（CD33、CD44）和甲状腺自身抗体（Anti-TPO、Anti-Tg）之间的关系。正态性检验证实了数据的非参数分布，需要采用Mann-Whitney U检验进行组间比较。与对照组相比，BC-HT患者表现出TSH、CD33、Anti-TPO和Anti-Tg水平显著升高，同时T3和T4水平降低，提示甲状腺功能障碍。Spearman的相关分析显示，对照组TSH和T3/T4之间存在很强的负相关，而BC-HT中不存在这种负相关，表明正常的甲状腺反馈机制受到破坏。此外，CD33和CD44与甲状腺激素的相关性在对照组中很明显，但在BC-HT中不存在，这突出了免疫-甲状腺相互作用的改变。ROC分析显示TSH、Anti-Tg和Anti-TPO具有较高的诊断效能，灵敏度超过0.75，而CD33和CD44的诊断效用有限。这些发现提示BC-HT患者存在明显的免疫-甲状腺失调，并强调了甲状腺特异性标志物在疾病分层中的潜力。未来的研究应侧重于纵向研究和机制调查，以进一步描述免疫标志物在甲状腺自身免疫背景下乳腺癌病理生理中的作用。

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引用次数: 0

Evaluation of the quality of bovine semen subjected to cryopreservation with hydroxypropylmethylcellulose 用羟丙基甲基纤维素冷冻保存牛精液的质量评价

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-06-01 Epub Date: 2025-03-27 DOI: 10.1016/j.repbio.2025.101013

Anna Monallysa Silva de Oliveira , Nívia Maria Rocha Brandão , Anailson De Oliveira Maciel , Nágylla Santos De Almeida , Nayonara Santos De Almeida , Thiago Santos Santos , Izakiel Reis Marinho , Francisco Cardoso Figueiredo , Yndyra Nayan Teixeira Carvalho Castelo Branco , José Adalmir Torres de Souza

The objective was to evaluate the effects of different concentrations of hydroxypropylmethylcellulose (HPMC) on the cryopreservation of bovine semen. Semen was collected from seven bulls by electroejaculation and diluted in Tris-egg yolk medium with and without glycerol (G) in the following treatments: C: Tris-Egg Yolk + 6 %G; T1: Tris-Egg Yolk + 3 % G; T2: Tris-Egg Yolk + 0 % G; T3: Tris-Egg Yolk + 0.5 % HPMC; T4: Tris-Egg Yolk + 1.0 % HPMC; T5: Tris-Egg Yolk + 1.5 % HPMC; T6: Tris-Egg Yolk + 5.5 % G + 0.5 % HPMC. Samples were frozen and stored at −196°C. After thawing, sperm quality was assessed using the rapid thermoresistance test (RTRT), sperm morphology, hypoosmotic swelling test, plasma membrane integrity, mitochondrial activity, and in vitro fertilization (IVF) test. RTRT results showed that the control group had better motility and vigor at 0’, 15’, and 30’ compared to the T6 group. The T6 group had better results than other groups supplemented with 0.5 %, 1.0 %, and 1.5 % HPMC. No significant differences were observed at 45’ for RTRT, hypoosmotic swelling, morphology, membrane integrity, mitochondrial activity, or IVF rates. In conclusion, the addition of HPMC reduced sperm motility and vigor but did not negatively affect other cryopreservation parameters.

目的是评价不同浓度羟丙基甲基纤维素（HPMC）对牛精液冷冻保存的影响。采用电射精法采集7头公牛的精液，在添加和不添加甘油(G)的tris -卵黄培养基中稀释，处理如下：C: tris -卵黄+ 6 %G；T1: Tris-Egg yellow + 3 % G；T2: Tris-Egg yellow + 0 % G；T3: tris -蛋黄+ 0.5 % HPMC；T4: tris -蛋黄+ 1.0 % HPMC；T5: tris -蛋黄+ 1.5 % HPMC；T6: tris -蛋黄+ 5.5 % G + 0.5 % HPMC。样品冷冻保存于- 196°C。解冻后，通过快速耐热性试验（RTRT）、精子形态、低渗肿胀试验、质膜完整性、线粒体活性和体外受精（IVF）试验评估精子质量。RTRT结果显示，与T6组相比，对照组在0 ‘,15 ’和30 '时具有更好的运动性和活力。T6组效果优于添加0.5 %、1.0 %、1.5 % HPMC组。在45 '时，RTRT、低渗肿胀、形态学、膜完整性、线粒体活性或体外受精率均无显著差异。总之，HPMC的加入降低了精子活力和活力，但对其他低温保存参数没有负面影响。

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引用次数: 0

Fucoxanthin attenuates LPS-induced endometritis via inhibiting inflammatory factors through the NF-κB pathway 岩藻黄素通过NF-κB途径抑制炎症因子，减轻脂多糖诱导的子宫内膜炎

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-06-01 Epub Date: 2025-03-24 DOI: 10.1016/j.repbio.2025.101010

Qin Yao, Yuejuan Zhang, Qizhi Yan, Junhui Qian

Endometritis is an infectious disease of the female reproductive system and commonly treated with antibiotics. However, the high resistance rates to antibiotics necessitate the urgent research for new and effective therapeutic strategies. The aim of this research is to explore the effect of fucoxanthin (FX) on endometritis through in vitro and in vivo assays. The effect of FX on inflammation was first explored in vitro using LPS-induced bovine endometrial epithelial (BEND) cell injury model. After the anti-inflammation effect of FX was confirmed in vitro, the effect of FX on endometritis was investigated in vivo using LPS-induced mice model. The female mice were randomly assigned into control, control + FX, LPS, and LPS + FX (100, 200 mg/kg) groups. The histological features of the uterus and expression levels of NF-κBp65 and inflammatory mediators (COX-2, iNOS, IL-1β, IL-6, and TNF-α) in the uterine tissue were compared among the animal groups. Our in vitro results showed that LPS induced BEND cell damage while significantly enhancing the expression of NF-κBp65 and inflammatory mediators (COX-2, iNOS, IL-1β, IL-6, and TNF-α). Nevertheless, pretreatment with FX reversed the abnormal phenomena caused by LPS. In vivo, LPS treatment resulted in obvious histopathological uterus damages, which were alleviated by FX treatment. Consistent with the in vitro assay, FX treatment also inhibited the expression of NF-κBp65 and inflammatory mediators in the animal experiments. Our study implies that FX is a potential therapeutic agent for endometritis. The beneficial function of FX on endometritis was achieved by inhibiting the inflammatory factors through the NF-κB pathway.

子宫内膜炎是一种女性生殖系统的感染性疾病，通常用抗生素治疗。然而，抗生素的高耐药率迫切需要研究新的有效的治疗策略。本研究的目的是通过体外和体内实验探讨岩藻黄素（FX）对子宫内膜炎的影响。采用lps诱导的牛子宫内膜上皮（BEND）细胞损伤模型，首次探讨了FX对炎症的影响。在体外实验证实FX的抗炎作用后，采用lps诱导小鼠模型，在体内研究FX对子宫内膜炎的作用。将雌性小鼠随机分为对照组、对照组+ FX、LPS和LPS + FX（100、200 mg/kg）组。比较各组大鼠子宫组织学特征及子宫组织中NF-κBp65及炎症介质COX-2、iNOS、IL-1β、IL-6、TNF-α的表达水平。我们的体外实验结果显示，LPS诱导BEND细胞损伤，同时显著提高NF-κBp65和炎症介质（COX-2、iNOS、IL-1β、IL-6和TNF-α）的表达。然而，FX预处理逆转了LPS引起的异常现象。在体内，LPS处理导致明显的组织病理学子宫损伤，FX处理减轻了这种损伤。与体外实验一致，动物实验中FX处理也抑制了NF-κBp65和炎症介质的表达。我们的研究表明FX是治疗子宫内膜炎的潜在药物。FX对子宫内膜炎的有益作用是通过NF-κB途径抑制炎症因子实现的。

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引用次数: 0

Chronic stress disturbs neuroendocrine control of reproduction and fertility in male rats 慢性应激干扰雄性大鼠生殖和生育的神经内分泌控制

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-05-24 DOI: 10.1016/j.repbio.2025.101027

Habit Medina , Alejandra Flores , Lizbeth Juárez-Rojas , Fahiel Casillas , Mohammad Mehdi Ommati , Reza Heidari , Sara Vázquez , Denise Clavijo-Cornejo , Sheila Peña-Corona , Socorro Retana-Márquez

Currently, stress is considered one of the risk factors for infertility in male humans, altering sperm function. Sperm production and maturation depends on the hypothalamic-pituitary-testis axis control. Therefore, the objective of the current study was to evaluate the effects of chronic stress on the neuroendocrine control of male reproduction, the oxidative status in the epididymis, and male fertility. Adult male rats were assigned to control or chronic stress groups. Chronically stressed males were exposed to cold-water immersion (CWI) for 50 consecutive days. After euthanasia, the hypothalamus was dissected for Kisspeptin (Kiss1) and Gonadotropin releasing hormone (GnRH) evaluation; serum luteinizing hormone (LH), testosterone (T), and corticosterone concentrations were determined. In the epididymis, reactive oxygen species (ROS), lipid peroxides, and content of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx4) were assessed. Sperm motility, viability, concentration, morphology and acrosomal reaction were assessed. Epididymal sperm were used for in-vitro fertilization with oocytes from intact female rats. Stressed males showed lower hypothalamic Kiss1 and GnRH content, lower LH and T concentration, together with higher serum corticosterone concentration. ROS production, and lipid peroxides increased in all epididymal regions, while SOD, CAT, and GPx4 content decreased after chronic stress; sperm quality was also lower. The percentage of fertilized oocytes decreased, and embryonic development was low, compared to controls. Together, these results show that chronic stress disrupts neuroendocrine control of male reproduction and generates oxidative stress in the epididymis. These effects disturb sperm quality, leading to low fertilizing potential and poor embryonic development.

目前，压力被认为是男性不育的危险因素之一，它会改变精子的功能。精子的产生和成熟取决于下丘脑-垂体-睾丸轴的控制。因此，本研究的目的是评估慢性应激对男性生殖神经内分泌控制、附睾氧化状态和男性生育能力的影响。将成年雄性大鼠分为对照组和慢性应激组。长期应激雄鼠连续50天浸泡在冷水中。安乐死后解剖下丘脑进行Kisspeptin （Kiss1）和促性腺激素释放激素（GnRH）评估；测定血清黄体生成素（LH）、睾酮(T)和皮质酮浓度。测定附睾中活性氧（ROS）、脂质过氧化物以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx4）的含量。评估精子活力、活力、浓度、形态和顶体反应。用完整雌鼠附睾精子与卵母细胞进行体外受精。应激雄性下丘脑Kiss1和GnRH含量降低，LH和T浓度降低，血清皮质酮浓度升高。慢性应激后附睾各区域ROS和脂质过氧化物含量均升高，SOD、CAT和GPx4含量降低；精子质量也较低。与对照组相比，受精卵的百分比下降，胚胎发育较低。总之，这些结果表明，慢性应激破坏了男性生殖的神经内分泌控制，并在附睾中产生氧化应激。这些影响会影响精子质量，导致受精率低和胚胎发育不良。

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引用次数: 0

Selection and validation of reference genes for gene expression studies of decidualization based on RNA sequencing 基于RNA测序的脱个体化基因表达研究内参基因的选择与验证

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-04-28 DOI: 10.1016/j.repbio.2025.101023

Haoyu Yang , Cancan Wang , Wenxuan Li , Xin Su , Mengyuan Li , Qian Li , Xiang-Hong Xu , Liping Jin

Decidualization is a multistep and complex physiological process used to aid the development of an implanting embryo. To date, the potential genes regulating decidualization have not been elucidated. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is widely used in gene expression studies, with relative quantification being the predominant method due to its simplicity, cost-effectiveness, and lower sample requirements. This method determines gene expression levels by normalizing to reference genes. However, the selection of stable reference genes for studies on decidualization remains a challenge. Based on the RNA-seq dataset from human endometrial stromal cells (ESCs) and differentiated ESCs (DESCs), ten new candidate reference genes were identified. The expression of these ten candidates, along with the commonly used reference gene β-actin, was measured in ESCs, DESCs, and decidual stromal cells (DSCs) through RTqPCR. Five algorithms were used to systematically identify suitable reference genes. The results indicated that Staufen double-stranded RNA binding protein 1 (STAU1) was most stable for induced decidualization in vitro, showing consistent expression in ESCs and DSCs. Using STAU1 as the reference gene, the expression levels of insulin like growth factor binding protein 1 and prolactin in DESCs were significantly higher than those in ESCs. Stau1 was further validated with both natural pregnancy and artificially induced decidualization mouse models. Based on our bioinformatics analysis, we also propose that kelch like family member 9 and TSC complex subunit 1 may serve as additional reference genes. Our findings offer valuable insights for gene expression studies of endometrial decidualization.

脱个体化是一个多步骤和复杂的生理过程，用于帮助着床胚胎的发育。迄今为止，调节去个体化的潜在基因尚未被阐明。逆转录定量聚合酶链反应（RT-qPCR）广泛应用于基因表达研究，相对定量方法因其简单、成本效益高、样品要求低而成为主要方法。这种方法通过规范化参考基因来确定基因表达水平。然而，选择稳定的内参基因用于脱个体化研究仍然是一个挑战。基于人子宫内膜基质细胞（ESCs）和分化ESCs （DESCs）的RNA-seq数据集，确定了10个新的候选内参基因。通过RTqPCR检测这10个候选基因以及常用的内参基因β-肌动蛋白在ESCs、DESCs和蜕质细胞（DSCs）中的表达。采用5种算法系统地筛选合适的内参基因。结果表明，Staufen双链RNA结合蛋白1 （STAU1）在体外诱导脱体细胞化中最稳定，在ESCs和dsc中表达一致。以STAU1为参比基因，DESCs中胰岛素样生长因子结合蛋白1和催乳素的表达水平明显高于ESCs。在自然妊娠和人工诱导脱胎小鼠模型中进一步验证了Stau1。基于生物信息学分析，我们还提出kelch样家族成员9和TSC复合体亚基1可能作为额外的内参基因。我们的发现为子宫内膜去个体化的基因表达研究提供了有价值的见解。

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引用次数: 0

Anti-mullerian hormone in felids: A systematic review 田间抗苗勒管激素：系统综述

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-04-19 DOI: 10.1016/j.repbio.2025.101016

Camila Lapuente , Augusto Lantermino , Sol Arioni , Paula G. Blanco , Cristina Gobello

Anti-Müllerian hormone (AMH) is a dimeric glycoprotein that belongs to the transforming growth factor-β (TGF-β) superfamily. This hormone that is produced by gonadal Sertoli cells in males and granulosa cells in females has been extensively studied in humans, rodents, and livestock species. Research on AMH in felids began in 2011 and given the increasing number of studies over recent years, an updated literature review is necessary to clarify and organize future research directions. The objective of this article was to conduct a systematic review of AMH in domestic and wild felids. From a literature search of international publications, 23 were selected for inclusion. AMH determinations were performed using commercial enzyme-linked immunosorbent assays (ELISA) and electrochemiluminescence immunoassays (EQLIA). In female felids, AMH concentrations decrease with age, along with follicular reserve diminution. AMH can also be used to diagnose granulosa cell ovarian tumors and cryptorchidism in females and males, respectively. This hormone serves as a marker for reproductive status and can reflect gonadal function in both genders. Furthermore, AMH may prove to be a valuable predictive tool for reproductive biotechnologies in both domestic and wild felids. Several aspects of this hormone still remain to be elucidated, including its variations throughout the estrous cycle and the effect of photoperiod. Finally, standardization of assays and the establishment of reference ranges for both domestic and wild animals are necessary for widespread clinical application and future research development.

抗勒氏激素（AMH）是一种二聚体糖蛋白，属于转化生长因子-β (TGF-β)超家族。这种激素是由雄性的性腺支持细胞和雌性的颗粒细胞产生的，已经在人类、啮齿动物和牲畜物种中进行了广泛的研究。田间AMH的研究始于2011年，近年来研究越来越多，有必要更新文献综述，以明确和组织未来的研究方向。本文的目的是进行系统综述AMH在国内和野生野地。从国际出版物的文献检索中，选择了23篇纳入。AMH测定采用商用酶联免疫吸附法（ELISA）和电化学发光免疫分析法（EQLIA）。在雌性田鼠中，AMH浓度随着年龄的增长而下降，伴随着卵泡储备的减少。AMH也可分别用于诊断女性和男性卵巢颗粒细胞瘤和隐睾症。这种激素是生殖状态的标志，可以反映两性的性腺功能。此外，AMH可能被证明是家畜和野生动物生殖生物技术的一个有价值的预测工具。这种激素的几个方面仍有待阐明，包括它在整个发情周期中的变化和光周期的影响。最后，标准化检测方法，建立家畜和野生动物的参考范围，是广泛临床应用和未来研究发展的必要条件。

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引用次数: 0

Tributyltin affects the growth of ovarian granulosa cells in polycystic ovary syndrome by upregulating YY1-mediated CDKN1C via the PI3K/AKT pathway 三丁基丁通过PI3K/AKT通路上调yy1介导的CDKN1C，影响多囊卵巢综合征卵巢颗粒细胞的生长

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-04-19 DOI: 10.1016/j.repbio.2025.101020

Shitao Dong, Youbin Liu, Zhimin Yang

Polycystic ovary syndrome (PCOS) poses a significant threat to women's fertility and quality of life. Studies have found a close association between the environmental contaminant tributyltin (TBT) and the occurrence of PCOS. The main objective of this study was to investigate the specific mechanisms by which TBT adversely affects the growth of ovarian granulosa cells. Cell viability, cycle, proliferation, and apoptosis were measured by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT), 5-ethynyl-2’-deoxyuridine (EdU), and flow cytometry. Simultaneously, lactate dehydrogenase (LDH) leakage and Caspase-3 activity were measured by the corresponding kits. Besides, western blot was used to analyze the protein levels of cyclin-dependent kinase inhibitor 1 C (CDKN1C) and the transcription factor Yin Yang 1 (YY1). TBT severely impaired the viability, cell cycle, and proliferation capacity of granulosa cells, and induced their apoptosis. Silencing CDKN1C and YY1 alleviated the damage caused by TBT to the cells, but these repair effects were weakened by CDKN1C overexpressed. By inhibiting the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway, TBT upregulated the YY1-mediated CDNK1C, and further exacerbated the damage to granulosa cells. This study revealed the mechanism that TBT induced the loss of ovarian granulosa cells in PCOS patients by upregulating YY1-mediated CDKN1C expression, which provided new ideas and targets for the pathogenesis and treatment of PCOS.

多囊卵巢综合征（PCOS）严重威胁妇女的生育能力和生活质量。研究发现环境污染物三丁基锡（TBT）与多囊卵巢综合征的发生密切相关。本研究的主要目的是探讨TBT对卵巢颗粒细胞生长不利的具体机制。采用3-（4,5 -二甲基-2-噻唑基）- 2,5 -二苯基-2- h -溴化四唑（MTT）、5-乙基-2 ' -脱氧尿苷（EdU）和流式细胞术检测细胞活力、周期、增殖和凋亡。同时用相应的试剂盒检测乳酸脱氢酶（LDH）渗漏量和Caspase-3活性。western blot检测细胞周期蛋白依赖性激酶抑制剂1 C （CDKN1C）和转录因子阴阳1 （YY1）蛋白水平。TBT严重损害颗粒细胞的活力、细胞周期和增殖能力，诱导颗粒细胞凋亡。沉默CDKN1C和YY1可减轻TBT对细胞的损伤，但这些修复作用因CDKN1C过表达而减弱。TBT通过抑制磷脂酰肌醇3-激酶/蛋白激酶B （PI3K/AKT）信号通路，上调yy1介导的CDNK1C，进一步加重对颗粒细胞的损伤。本研究揭示了TBT通过上调yy1介导的CDKN1C表达诱导PCOS患者卵巢颗粒细胞丢失的机制，为PCOS的发病机制和治疗提供了新的思路和靶点。

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引用次数: 0

Evaluation of mitochondrial copy number and gene expression changes in the spermatozoa of buffalo bulls under heat stress 热应激条件下水牛精子线粒体拷贝数和基因表达变化的评价

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-04-02 DOI: 10.1016/j.repbio.2025.101014

Meenakshi Chitkara , Harsimran Kaur , Rashi Vasisth , Karpenahalli Ranganatha Sriranga , Ankita Gurao , Karan Mahar , Mahesh Shivanand Dige , R.A.K. Aggarwal , Manishi Mukesh , Pradeep Kumar , Pawan Singh , Ranjit Singh Kataria

Relative copy number of mitochondria was estimated for the potential association with the expression of mitochondrial coded stress related genes in bubaline spermatozoa. Semen samples were collected from buffalo bulls under two extreme temperature-humidity index conditions: hot summer and winter. Based on the semen quality, the bulls were categorized: exhibiting poor semen quality during hot summer as "seasonally affected," while those maintaining good semen quality throughout the year, as "seasonally not affected". The average mitochondrial copy numbers were lower during hot summer (15.42 ± 1.2368) compared to winter (17.29 ± 0.72) in both the groups. Furthermore, within the hot summer period, bulls classified as seasonally affected exhibited significantly lower mitochondrial copy numbers (12.86 ± 1.343) than their seasonally unaffected counterpart (17.97 ± 1.34). These results suggest a potential role of mitochondria in influencing semen quality, particularly in response to impaired scrotal thermoregulation during the summer season. Although the fold change in apoptotic genes (BCL2, MCL1, CASP3, and BAK) and oxidative panel genes (CAT, SOD, GPx, ATF4, and FOXO-3), did not differ significantly across the groups, differences were observed between the seasons. Further, to understand the role of copy number in apoptosis and ROS scavenging across the seasons and the groups, the generalized mixed model was employed. The results conveyed a significant negative interaction of copy number with the expression of CAT gene and significant positive interaction of copy number with the apoptotic gene panel. Our findings underscore the significant role of mitochondrial copy numbers in domestic buffalo spermatozoa in managing the challenges of thermoregulation posed by harsh tropical conditions.

估计线粒体的相对拷贝数与线粒体编码应激相关基因表达的潜在关联。在炎热的夏季和冬季两种极端温度湿度指数条件下采集水牛的精液样本。根据精液质量，公牛被分类为：在炎热的夏季表现出较差的精液质量为“季节性影响”，而全年保持良好精液质量的公牛为“不受季节性影响”。两组在炎热夏季的平均线粒体拷贝数（15.42 ± 1.2368）均低于冬季（17.29 ± 0.72）。此外，在炎热的夏季，受季节性影响的公牛的线粒体拷贝数（12.86 ± 1.343）明显低于未受季节性影响的公牛（17.97 ± 1.34）。这些结果表明，线粒体在影响精液质量中的潜在作用，特别是在夏季阴囊温度调节受损的情况下。虽然凋亡基因（BCL2, MCL1， CASP3和BAK）和氧化面板基因（CAT， SOD, GPx， ATF4和FOXO-3）的折叠变化在组间没有显著差异，但在季节之间观察到差异。此外，为了了解拷贝数在不同季节和不同组细胞凋亡和ROS清除中的作用，我们采用了广义混合模型。结果表明，拷贝数与CAT基因表达呈显著负交互作用，与凋亡基因表达呈显著正交互作用。我们的研究结果强调了水牛精子线粒体拷贝数在应对热带恶劣条件下的体温调节挑战中的重要作用。

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引用次数: 0

The impact of extracellular glucose concentrations on antioxidant capacity, viability, and microRNA expression in TM4 Sertoli cells 细胞外葡萄糖浓度对TM4支持细胞抗氧化能力、活力和microRNA表达的影响

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-03-30 DOI: 10.1016/j.repbio.2025.101015

Farha A. Ali Shafi , Ali Thoulfikar A. Imeer , Hassan Ali Abood Nassrullah , Ali Mutashar Naeemah

This study investigates the impact of extracellular glucose concentrations on antioxidant capacity, viability, and microRNA (miR) expression in TM4 Sertoli cell lines. TM4 cells were cultured in high-glucose (115 µm) and low-glucose (<505 µm) conditions to simulate hyperglycemia and glucose starvation, respectively. The study measured total antioxidant capacity (TAC), malondialdehyde (MDA), total oxidant status (TOS), glutathione (GSH), glutathione disulfide (GSSG), NADP/NADPH, glutathione peroxidase (GPX), and glutathione reductase (GR) levels. MiR-17, miR-34, miR-106a, and miR-200a expression levels were assessed. Cell viability and apoptosis were evaluated using MTT assay and acridine-orange staining. Results indicated that high glucose reduced miR-17 expression while low glucose increased it. Both glucose conditions elevated miR-34, miR-106a, and miR-200a expressions. TAC levels decreased, while TOS and MDA levels increased significantly under both conditions. High glucose had no significant effect on GPX and GR levels, whereas low glucose decreased them. Both conditions led to reduced GSH levels, increased GSSG levels, and altered NADP/NADPH ratio. Increased apoptosis and decreased cell viability were observed under both glucose conditions. These findings suggest that extracellular glucose levels significantly dysregulate miRNA expression, antioxidant capacities, and redox buffer systems in TM4 cells. High glucose conditions suppress miR-17 expression, increase miR-34 and miR-106a levels, and induce reductive buffer imbalance. Conversely, low glucose conditions trigger compensatory mechanisms via increased miR-17 expression to enhance antioxidant status while reducing GPX and GR levels. These results provide insights into the molecular responses of Sertoli cells under varying glucose environments, highlighting potential therapeutic pathways for conditions like diabetes and metabolic dysfunctions.

本研究探讨了细胞外葡萄糖浓度对TM4支持细胞系抗氧化能力、活力和microRNA （miR）表达的影响。分别在高糖（115 µm）和低糖（505 µm）条件下培养TM4细胞，模拟高血糖和葡萄糖饥饿。研究测量了总抗氧化能力（TAC）、丙二醛（MDA）、总氧化状态（TOS）、谷胱甘肽（GSH）、谷胱甘肽二硫（GSSG）、NADP/NADPH、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽还原酶（GR）水平。评估MiR-17、miR-34、miR-106a和miR-200a的表达水平。MTT法和吖啶橙染色法检测细胞活力和凋亡情况。结果表明，高糖降低miR-17的表达，低糖升高miR-17的表达。两种葡萄糖条件均升高miR-34、miR-106a和miR-200a的表达。在两种情况下，TAC水平均下降，而TOS和MDA水平均显著升高。高糖对GPX和GR水平无显著影响，低糖使其降低。两种情况都导致GSH水平降低，GSSG水平升高，NADP/NADPH比值改变。在两种葡萄糖条件下，细胞凋亡增加，细胞活力下降。这些发现表明，细胞外葡萄糖水平显著失调了TM4细胞的miRNA表达、抗氧化能力和氧化还原缓冲系统。高糖条件抑制miR-17表达，增加miR-34和miR-106a水平，并诱导还原性缓冲失衡。相反，低糖条件通过增加miR-17表达来触发代偿机制，从而增强抗氧化状态，同时降低GPX和GR水平。这些结果提供了Sertoli细胞在不同葡萄糖环境下的分子反应的见解，突出了糖尿病和代谢功能障碍等疾病的潜在治疗途径。

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The special phenotypic characteristics of Dummerstorf superfertile mouse lines could depend on the expression levels of IGF-axis genes Dummerstorf超育小鼠系的特殊表型特征可能与igf轴基因的表达水平有关

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-03-29 DOI: 10.1016/j.repbio.2025.101012

Michela Calanni-Pileri , Joachim M. Weitzel , Andreas Hoeflich , Martina Langhammer , Marten Michaelis

To date, animal models with reproductive phenotypes are knockout or transgenic and typically exhibit reduced fertility or infertility. This limits research to studying single-gene effects or loss of fertility. By contrast, Dummerstorf high-fertility mouse lines 1 and 2 (FL1 and FL2) are two unique outbred selection models that demonstrate exceptional reproductive performance. After approximately 50 years of selection, both lines have doubled the number of ovulated oocytes per cycle and consequently their litter size (>20 vs ∼11) compared to the unselected mice of the same founder population (Dummerstorf FZTDU, ctrl line). FL1 and FL2 exhibit atypical estrous cycle length and altered levels of hormones, such as insulin and leptin, which are associated with GnRH release and/or increased body fat content. Unlike typical cases where these factors impair fertility, they instead contribute to the FLs’ high reproductive capacity: the increased ovulation rate results from an upgrade in the quality of their oocytes, influenced by different ovarian lipid profile. In the present study, we analyzed the expression of IGF-axis marker genes linked to reproductive performance and FL-specific traits in three tissues. We found that lepr, which plays a critical role in implantation, was upregulated in the FL1 uterus (1.5-fold vs. ctrl, p < 0.05). In FL1 follicles, igf1, IGF-biding proteins (IGFBP2, IGFBP4) and hsf1—which is involved in gametogenesis—were significantly upregulated (1–4-fold vs. ctrl, p < 0.05 for igf1, hsf1 and IGFBP4; p < 0.01 for igfbp2). In FL2, uterine size was reduced relatively to the body weight (∼0.2 % FL2 vs. 0.25 % in ctrl and 0.28 % in FL1, p < 0.001), indicating that uterus dimensions do not drive their increased litter size. These findings provide new insights into the molecular basis of high fertility and could serve as a foundation for further studies on genotype-phenotype relationships in reproductive biology.

迄今为止，具有生殖表型的动物模型是基因敲除或转基因的，通常表现为生育力降低或不育。这限制了对单基因影响或生育能力丧失的研究。相比之下，Dummerstorf高育性小鼠系1和2 （FL1和FL2）是两种独特的远交选择模型，表现出卓越的生殖性能。经过大约50年的选择，两个品系每个周期的排卵卵母细胞数量都增加了一倍，因此它们的产仔数（>20 vs ~ 11）与未选择的相同创始种群（Dummerstorf FZTDU，对照线）相比。FL1和FL2表现出非典型的发情周期长度和激素水平的改变，如胰岛素和瘦素，这与GnRH释放和/或体脂含量增加有关。与这些因素影响生育能力的典型情况不同，它们反而有助于fl的高生殖能力：排卵率的增加是由于卵母细胞质量的提高，受不同卵巢脂质谱的影响。在本研究中，我们分析了与生殖性能和fl特异性性状相关的igf轴标记基因在三种组织中的表达。我们发现，在胚胎植入过程中起关键作用的lepr在FL1子宫中表达上调（1.5倍，p <； 0.05）。在FL1卵泡中，igf1、igf结合蛋白（IGFBP2、IGFBP4）和参与配子发生的hsf1显著上调（1 - 4倍于对照，p <； 0.05）；P <； 0.01,igfbp2)。在FL2中，子宫尺寸相对于体重减小（FL2为0.2 %，对照组为0.25 %，FL1为0.28 %,p <； 0.001），表明子宫尺寸不会导致产仔数的增加。这些发现为研究高育性的分子基础提供了新的见解，并可为进一步研究生殖生物学中基因型-表型关系奠定基础。

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引用次数: 0