Reproductive biology最新文献

英文中文

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-01-01

引用次数: 0

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-01-01

引用次数: 0

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-01-01

引用次数: 0

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-01-01

引用次数: 0

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-01-01

引用次数: 0

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-01-01

引用次数: 0

Endometrial immune profile: A predictor of pregnancy success 子宫内膜免疫特征：妊娠成功的预测因子

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-12-31 DOI: 10.1016/j.repbio.2025.101174

Sina Baharaghdam , Shima Karimi , Mohammad Esfini Farahani , Leili Aghebati-Maleki , Mehdi Yousefi

The likelihood of a successful pregnancy is influenced by a set of variables that influence endometrial receptivity, including hormonal, genetic, metabolic, age, lifestyle, and immunological factors. Among these, the endometrial immune profile has received particular attention as a critical actor of implantation and embryo tolerance. Dynamic fluctuations in immune cell populations—such as macrophages, dendritic cells, uterine natural killer cells, and regulatory T cells—across the menstrual cycle might significantly affect the endometrium's capacity to support successful implantation. Recent evidence highlights that disruptions in the quantity, phenotype, or function of these immune cells contribute to impaired endometrial receptivity in infertility-related disorders, including recurrent implantation failure, recurrent pregnancy loss, endometriosis, and polycystic ovary syndrome. This review provides a comprehensive assessment of immune cell composition and function in the healthy endometrium compared to pathological conditions, emphasizing how immune dysregulation may impair pregnancy. Furthermore, we evaluate both current and emerging diagnostic modalities—from immunohistochemistry and flow cytometry to high-resolution single-cell transcriptomics—and endometrial microbiome impact on immune profiling that enables more precise characterization of immune profile dysregulation, alongside established and investigational therapies, with particular attention to their efficacy and mechanistic rationale. By integrating these insights, a clinically oriented framework can be provided to guide the development of personalized diagnostic algorithms and targeted immune-based therapies that, ultimately, could lead to improved implantation rates and live birth outcomes in individuals facing infertility disorders with immunological causes.

成功怀孕的可能性受到一系列影响子宫内膜容受性的变量的影响，包括激素、遗传、代谢、年龄、生活方式和免疫因素。其中，子宫内膜免疫谱作为植入和胚胎耐受的关键因素受到了特别的关注。免疫细胞群（如巨噬细胞、树突状细胞、子宫自然杀伤细胞和调节性T细胞）在月经周期中的动态波动可能会显著影响子宫内膜支持成功植入的能力。最近的证据强调，这些免疫细胞的数量、表型或功能的破坏有助于不孕相关疾病的子宫内膜容受性受损，包括反复植入失败、反复妊娠丢失、子宫内膜异位症和多囊卵巢综合征。这篇综述提供了健康子宫内膜中免疫细胞组成和功能的综合评估，并与病理状况进行了比较，强调了免疫失调如何损害妊娠。此外，我们评估了当前和新兴的诊断模式-从免疫组织化学和流式细胞术到高分辨率单细胞转录组学-子宫内膜微生物组对免疫谱的影响，能够更精确地表征免疫谱失调，以及已建立和正在研究的治疗方法，特别关注其疗效和机制原理。通过整合这些见解，可以提供一个临床导向的框架来指导个性化诊断算法和靶向免疫治疗的发展，最终可以提高因免疫原因导致的不孕症患者的着床率和活产结果。

{"title":"Endometrial immune profile: A predictor of pregnancy success","authors":"Sina Baharaghdam , Shima Karimi , Mohammad Esfini Farahani , Leili Aghebati-Maleki , Mehdi Yousefi","doi":"10.1016/j.repbio.2025.101174","DOIUrl":"10.1016/j.repbio.2025.101174","url":null,"abstract":"<div><div>The likelihood of a successful pregnancy is influenced by a set of variables that influence endometrial receptivity, including hormonal, genetic, metabolic, age, lifestyle, and immunological factors. Among these, the endometrial immune profile has received particular attention as a critical actor of implantation and embryo tolerance. Dynamic fluctuations in immune cell populations—such as macrophages, dendritic cells, uterine natural killer cells, and regulatory T cells—across the menstrual cycle might significantly affect the endometrium's capacity to support successful implantation. Recent evidence highlights that disruptions in the quantity, phenotype, or function of these immune cells contribute to impaired endometrial receptivity in infertility-related disorders, including recurrent implantation failure, recurrent pregnancy loss, endometriosis, and polycystic ovary syndrome. This review provides a comprehensive assessment of immune cell composition and function in the healthy endometrium compared to pathological conditions, emphasizing how immune dysregulation may impair pregnancy. Furthermore, we evaluate both current and emerging diagnostic modalities—from immunohistochemistry and flow cytometry to high-resolution single-cell transcriptomics—and endometrial microbiome impact on immune profiling that enables more precise characterization of immune profile dysregulation, alongside established and investigational therapies, with particular attention to their efficacy and mechanistic rationale. By integrating these insights, a clinically oriented framework can be provided to guide the development of personalized diagnostic algorithms and targeted immune-based therapies that, ultimately, could lead to improved implantation rates and live birth outcomes in individuals facing infertility disorders with immunological causes.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"26 1","pages":"Article 101174"},"PeriodicalIF":2.5,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145883681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomal ncRNAs in seminal fluid: Unraveling their regulatory roles in male infertility 精液外泌体ncRNAs：揭示其在男性不育中的调节作用

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-12-31 DOI: 10.1016/j.repbio.2025.101175

Marwah Suliman Maashi

By transmitting bioactive substances, including proteins, lipids, and a varied spectrum of non-coding RNAs, seminal exosomes—including epididymosomes and prostasomes—are shown to be crucial in sperm maturation, motility, and protection. The paper emphasizes how miRNAs, lncRNAs, and circRNAs within these exosomes affect gene expression during spermatogenesis and are starting to be interesting non-invasive biomarkers for several infertility abnormalities, including azoospermia and asthenozoospermia. It also addresses the therapeutic possibilities of exosome-based approaches in reducing inflammation, oxidative stress, and cellular damage in the male reproductive system. The paper identifies essential information gaps and suggests future research paths to clarify the molecular processes behind male infertility by combining knowledge from proteomic, lipidomic, and transcriptomic investigations. These results help to close the knowledge gap on seminal exosomes and open the door for creative therapeutic and diagnostic tools in reproductive medicine.

通过传递生物活性物质，包括蛋白质、脂质和各种非编码rna，精子外泌体（包括附睾和前列腺体）在精子成熟、运动和保护中起着至关重要的作用。本文强调了这些外泌体中的miRNAs、lncRNAs和circRNAs如何影响精子发生过程中的基因表达，并开始成为几种不孕症异常（包括无精子症和弱精子症）的有趣的非侵入性生物标志物。它还解决了基于外泌体的治疗方法在减少炎症、氧化应激和男性生殖系统细胞损伤方面的可能性。本文确定了必要的信息缺口，并提出了未来的研究路径，以阐明男性不育背后的分子过程，通过结合蛋白质组学，脂质组学和转录组学研究的知识。这些结果有助于缩小对精液外泌体的知识差距，并为生殖医学的创造性治疗和诊断工具打开大门。

引用次数: 0

The secretome from human amniotic membrane-derived mesenchymal stem cells alleviate oxidative damage in bovine sperm 人羊膜间充质干细胞分泌组可减轻牛精子的氧化损伤

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-12-31 DOI: 10.1016/j.repbio.2025.101178

Negin Amirjannati , Mohammadmehdi Barfar , Mostafa Pournourali , Niloofar Khalili , Parviz Tajik , Razie Shams , Alireza Jahandideh , Leila Balaei Goli , Seyed Jafar Hashemian , Ablofazl Shirazi , Hannaneh Golshahi

Bovine spermatozoa are highly vulnerable to oxidative stress (OS), which can impair motility, viability, membrane integrity, and DNA integrity. Human amniotic membrane-derived mesenchymal stem cells (AM-MSCs) secrete paracrine factors with antioxidant properties, making their secretome (AM-MSC-Sec) a potential tool for protecting sperm function. This study evaluated the protective effects of AM-MSC-Sec on bovine sperm under OS. Frozen Holstein bull semen was thawed, purified using a two-layer density gradient, and divided into four groups: control (no treatment), H₂O₂ (10 μM), H₂O₂ + 20 % AM-MSC-Sec, and H₂O₂ + 20 % DMEM/F12. After 2 h at 38.5°C and 5 % CO₂, sperm quality parameters—including motility, progressive motility, viability, plasma membrane integrity, acrosome reaction, DNA fragmentation, malondialdehyde (MDA), and total antioxidant capacity (TAC)—were assessed. AM-MSC-Sec significantly improved the percentage of total and progressive motility, enhanced viability and membrane integrity, and decreased DNA fragmentation compared with control and DMEM groups. The percentage of acrosome-reacted sperm increased, and TAC levels were elevated, indicating improved antioxidant defense. DMEM/F12 alone did not show a protective effect. MDA levels increased non-significantly under stress in the control group; however, the difference was not statistically significant. These findings suggest that AM-MSC-Sec could mitigate oxidative damage and preserve multiple functional parameters in bovine sperm. AM-MSC-Sec may serve as a promising therapeutic agent for maintaining sperm quality, with potential applications in bovine reproductive technologies.

牛精子非常容易受到氧化应激（OS）的影响，氧化应激会损害精子的运动性、活力、膜完整性和DNA完整性。人羊膜间充质干细胞（AM-MSCs）分泌具有抗氧化特性的旁分泌因子，使其分泌组（AM-MSCs - sec）成为保护精子功能的潜在工具。本研究评价了AM-MSC-Sec对OS下牛精子的保护作用。冷冻荷斯坦公牛精液解冻，采用双层密度梯度纯化，分为4组：对照组（未处理）、H₂O₂（10 μM）、H₂O₂+ 20 % AM-MSC-Sec和H₂O₂+ 20 % DMEM/F12。在38.5°C和5 % CO₂条件下2 h后，评估精子质量参数，包括运动性、渐进式运动性、活力、质膜完整性、顶体反应、DNA断裂、丙二醛（MDA）和总抗氧化能力（TAC）。与对照组和DMEM组相比，AM-MSC-Sec显著提高了总运动性和进行运动性的百分比，提高了细胞活力和膜完整性，减少了DNA断裂。顶体反应精子的百分比增加，TAC水平升高，表明抗氧化防御能力增强。单独的DMEM/F12没有显示出保护作用。应激条件下，对照组丙二醛水平无明显升高；然而，差异无统计学意义。这些结果表明，AM-MSC-Sec可以减轻牛精子的氧化损伤，并保持多种功能参数。AM-MSC-Sec可能是一种很有前景的精子质量维持剂，在牛生殖技术中具有潜在的应用前景。

{"title":"The secretome from human amniotic membrane-derived mesenchymal stem cells alleviate oxidative damage in bovine sperm","authors":"Negin Amirjannati , Mohammadmehdi Barfar , Mostafa Pournourali , Niloofar Khalili , Parviz Tajik , Razie Shams , Alireza Jahandideh , Leila Balaei Goli , Seyed Jafar Hashemian , Ablofazl Shirazi , Hannaneh Golshahi","doi":"10.1016/j.repbio.2025.101178","DOIUrl":"10.1016/j.repbio.2025.101178","url":null,"abstract":"<div><div>Bovine spermatozoa are highly vulnerable to oxidative stress (OS), which can impair motility, viability, membrane integrity, and DNA integrity. Human amniotic membrane-derived mesenchymal stem cells (AM-MSCs) secrete paracrine factors with antioxidant properties, making their secretome (AM-MSC-Sec) a potential tool for protecting sperm function. This study evaluated the protective effects of AM-MSC-Sec on bovine sperm under OS. Frozen Holstein bull semen was thawed, purified using a two-layer density gradient, and divided into four groups: control (no treatment), H₂O₂ (10 μM), H₂O₂ + 20 % AM-MSC-Sec, and H₂O₂ + 20 % DMEM/F12. After 2 h at 38.5°C and 5 % CO₂, sperm quality parameters—including motility, progressive motility, viability, plasma membrane integrity, acrosome reaction, DNA fragmentation, malondialdehyde (MDA), and total antioxidant capacity (TAC)—were assessed. AM-MSC-Sec significantly improved the percentage of total and progressive motility, enhanced viability and membrane integrity, and decreased DNA fragmentation compared with control and DMEM groups. The percentage of acrosome-reacted sperm increased, and TAC levels were elevated, indicating improved antioxidant defense. DMEM/F12 alone did not show a protective effect. MDA levels increased non-significantly under stress in the control group; however, the difference was not statistically significant. These findings suggest that AM-MSC-Sec could mitigate oxidative damage and preserve multiple functional parameters in bovine sperm. AM-MSC-Sec may serve as a promising therapeutic agent for maintaining sperm quality, with potential applications in bovine reproductive technologies.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"26 1","pages":"Article 101178"},"PeriodicalIF":2.5,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145883680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ProAKAP4 as a potential biomarker of fertility and sperm freezability in males of different species - A review ProAKAP4作为不同物种雄性生殖能力和精子冷冻性的潜在生物标志物的研究进展

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-12-30 DOI: 10.1016/j.repbio.2025.101176

Paulina Surmacz, Monika Petrajtis-Gołobów, Paula Kiełbik, Katarzyna Siewruk, Ewa Kautz-Wasilewska, Sławomir Giziński

In recent years, special attention has been paid to proAKAP4, the precursor of A-kinase anchoring protein 4 (AKAP4), which plays a crucial role in the structure and function of the sperm flagellum. This testis-specific protein forms the fibrous sheath and local signal transduction required for proper sperm capacitation and motility. Highly conserved across species, proAKAP4 levels are strongly correlated with fertility and sperm survivability post-thaw. This review summarizes the biological roles of proAKAP4 and its potential as a semen quality biomarker in various mammalian species, including humans, horses, bulls, boars, dogs, rodents and other species. We discuss structural and molecular aspects of proAKAP4 (e.g., prodomain, PKA interactions, phosphorylation sites), as well as its involvement in key signaling pathways controlling sperm movement, such as the cAMP-PKA cascade and calcium signaling. The review also outlines various methods for measuring proAKAP4, such as ELISA, Western blotting and immunocytochemistry. Particular attention is given to commercial assays, which allow rapid and species-specific quantification of proAKAP4, independent of seminal plasma or cryoprotectant presence. Although the data are promising, researchers highlight the need for further validation in larger populations under varying environmental conditions. Nonetheless, current findings suggest that proAKAP4 may emerge as a standard marker in andrological diagnostics and a valuable tool for selecting breeding males and optimizing semen cryopreservation procedures.

近年来，a激酶锚定蛋白4 （AKAP4）的前体proAKAP4在精子鞭毛的结构和功能中起着至关重要的作用。这种睾丸特异性蛋白形成纤维鞘和局部信号转导，这是精子获得和运动所需的。proAKAP4在物种间高度保守，其水平与受精能力和解冻后精子存活率密切相关。本文综述了proAKAP4在人类、马、牛、猪、狗、啮齿动物等哺乳动物中的生物学作用及其作为精液质量生物标志物的潜力。我们讨论了proAKAP4的结构和分子方面（如原结构域、PKA相互作用、磷酸化位点），以及它参与控制精子运动的关键信号通路，如cAMP-PKA级联和钙信号传导。本文还概述了检测proAKAP4的各种方法，如ELISA、Western blotting和免疫细胞化学。特别注意的是商业分析，它允许快速和物种特异性定量proAKAP4，独立于精浆或冷冻保护剂的存在。虽然这些数据很有希望，但研究人员强调，需要在不同环境条件下的更大人群中进一步验证。尽管如此，目前的研究结果表明，proAKAP4可能会成为男科诊断的标准标记，以及选择繁殖雄性和优化精液冷冻保存程序的有价值的工具。

{"title":"ProAKAP4 as a potential biomarker of fertility and sperm freezability in males of different species - A review","authors":"Paulina Surmacz, Monika Petrajtis-Gołobów, Paula Kiełbik, Katarzyna Siewruk, Ewa Kautz-Wasilewska, Sławomir Giziński","doi":"10.1016/j.repbio.2025.101176","DOIUrl":"10.1016/j.repbio.2025.101176","url":null,"abstract":"<div><div>In recent years, special attention has been paid to proAKAP4, the precursor of A-kinase anchoring protein 4 (AKAP4), which plays a crucial role in the structure and function of the sperm flagellum. This testis-specific protein forms the fibrous sheath and local signal transduction required for proper sperm capacitation and motility. Highly conserved across species, proAKAP4 levels are strongly correlated with fertility and sperm survivability post-thaw. This review summarizes the biological roles of proAKAP4 and its potential as a semen quality biomarker in various mammalian species, including humans, horses, bulls, boars, dogs, rodents and other species. We discuss structural and molecular aspects of proAKAP4 (e.g., prodomain, PKA interactions, phosphorylation sites), as well as its involvement in key signaling pathways controlling sperm movement, such as the cAMP-PKA cascade and calcium signaling. The review also outlines various methods for measuring proAKAP4, such as ELISA, Western blotting and immunocytochemistry. Particular attention is given to commercial assays, which allow rapid and species-specific quantification of proAKAP4, independent of seminal plasma or cryoprotectant presence. Although the data are promising, researchers highlight the need for further validation in larger populations under varying environmental conditions. Nonetheless, current findings suggest that proAKAP4 may emerge as a standard marker in andrological diagnostics and a valuable tool for selecting breeding males and optimizing semen cryopreservation procedures.</div></div>","PeriodicalId":21018,"journal":{"name":"Reproductive biology","volume":"26 1","pages":"Article 101176"},"PeriodicalIF":2.5,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

首页上一页

下一页尾页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Reproductive biology

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀