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Possible Impact of Human β-defensin 1 on sperm motility in infertile men with abnormal sperm parameters 人类β防御素 1 对精子参数异常的不育男性精子活力的可能影响
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-29 DOI: 10.1016/j.repbio.2024.100887
Amir Masoud Firouzabadi , Mohammad Ebrahim Rezvani , Fateme Zare , Hossein Azizian , Farzaneh Fesahat

Human β-defensins and interleukins may be auxiliary in sperm maturation. This cross-sectional study aimed to evaluate the expression of Human β-defensins 1 and 2, interleukins (ILs)− 10 and -18 genes in sperm, as well as seminal plasma levels of these two cytokines in subfertile men with different types of sperm abnormalities compared to those with normozoospermic men. Participants were separated into two experimental groups: the control group (n = 25) and the group with sperm abnormalities (SA) (n = 45). SA participants were further subdivided into the following groups with n = 15 individuals each: Teratozoospermia (T), Asthenoteratozoospermia (AT), and Oligoasthenoteratozoospermia (OAT) groups. The quantitative real-time polymerase chain reaction was used to quantify the mRNA levels of hBDs 1 and 2, IL-10, and IL-18 in sperm. The seminal plasma concentrations of IL-10 and IL-18 were measured by using the enzyme-linked immunosorbent assay technique. The mRNA expression of hBD-1 and IL-10 showed a significant decrease in the OAT compared to the controls (P < 0.0001 and P = 0.02, respectively). The lowest seminal plasma concentration of IL-10 belonged to the OAT (P = 0.04). ROC curve analysis showed a sensitivity, specificity, and cutoff value of 82.35%, 86.67%, and 0.63 for hBD-1 levels, respectively. A positive and significant correlation was found between hBD-1 expression and sperm motility and IL-10 expression rate and normal sperm morphology.Therefore, hBD-1 could be considered as the alternative biomaterial to pre-treatments of infertile men with abnormal sperm parameters, specifically OAT men, which led to improving the assisted reproduction success rate.

人类β-防御素和白细胞介素可能在精子成熟过程中起辅助作用。这项横断面研究旨在评估人类β-防御素1和2、白细胞介素(ILs)-10和-18基因在精子中的表达情况,以及与正常无精子男性相比,不同类型精子异常的亚育男性精浆中这两种细胞因子的水平。参与者被分为两个实验组:对照组(25 人)和精子异常组(45 人)。精子异常组的参与者又分为以下几组,每组 n = 15 人:畸形精子症(T)组、少精子症(AT)组和少精子症(OAT)组。采用实时聚合酶链反应定量检测精子中 hBDs 1 和 2、IL-10 和 IL-18 的 mRNA 水平。精浆中IL-10和IL-18的浓度采用酶联免疫吸附法测定。与对照组相比,OAT 中 hBD-1 和 IL-10 的 mRNA 表达量明显下降(分别为 P < 0.0001 和 P = 0.02)。OAT患者精浆中的IL-10浓度最低(P = 0.04)。ROC曲线分析显示,hBD-1水平的敏感性、特异性和临界值分别为82.35%、86.67%和0.63。hBD-1的表达与精子活力、IL-10的表达率与正常精子形态之间存在明显的正相关性。因此,hBD-1可作为一种替代生物材料,用于对精子参数异常的不育男性(尤其是OAT男性)进行预处理,从而提高辅助生殖的成功率。
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引用次数: 0
Immunomodulation of Ethyl pyruvate on gestational diabetes mellitus in Mice; The impact on Th17/Treg balance 丙酮酸乙酯对小鼠妊娠糖尿病的免疫调节作用;对 Th17/Treg 平衡的影响
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-24 DOI: 10.1016/j.repbio.2024.100861
Min Zhao , Xiuping Bian , Li Meng

Gestational diabetes mellitus (GDM) is recognized as one of the most common diseases among pregnant women and inflammatory responses can be a major reason for its induction and development. T helper 17 (Th17)/regulatory T cells (Tregs) imbalance resulting in the increased levels of pro-inflammatory and decreased levels of anti-inflammatory cytokines has been showed as major mechanisms involved in the pathogenesis of GDM. There are various treatment options, but none of them are completely therapeutic. Ethyl pyruvate (EP) is a stable derivate of pyruvate that showed anti-oxidant and anti-inflammatory properties in an in-vivo and in-vitro models. To examine the therapeutic efficacy of EP in GDM, mice were mated and EP (100 mg/kg) was administered intraperitoneally to C57BL/6 mice. EP-treated mice exhibited improved symptoms of GDM by decreased blood glucose levels and body-weight and increased insulin levels and insulin sensitivity. Furthermore, EP could significantly attenuate the impairments to offspring, including birth size and birth weight. The inflammatory responses were also decreased by EP through regulating the production of Th17-related cytokines, such as interleukin (IL)− 17 and IL-21. The levels of other inflammatory cytokines were also inhibited, including IL-1β, IL-6, and tumor necrosis factor (TNF)-α. In addition, it was found that EP increased the population of Tregs and Treg-related cytokines, IL-10 and transforming Growth Factor-β TGF-β, in GDM mice. In conclusion, EP could modulate GDM in mice and might be a potential therapeutic strategy candidate for the treatment of patients with GDM.

妊娠糖尿病(GDM)被认为是孕妇中最常见的疾病之一,而炎症反应可能是诱发和发展这种疾病的主要原因。T 辅助细胞 17(Th17)/调节性 T 细胞(Tregs)失衡导致促炎细胞因子水平升高,而抗炎细胞因子水平降低,已被证明是 GDM 发病的主要机制。目前有多种治疗方法,但没有一种是完全有效的。丙酮酸乙酯(EP)是丙酮酸的一种稳定衍生物,在体内和体外模型中显示出抗氧化和抗炎特性。为了研究 EP 对 GDM 的疗效,对 C57BL/6 小鼠进行交配并腹腔注射 EP(100 毫克/千克)。经 EP 治疗的小鼠血糖水平和体重下降,胰岛素水平和胰岛素敏感性增加,从而改善了 GDM 的症状。此外,EP 还能明显减轻对后代的影响,包括出生大小和出生体重。EP 还能通过调节 Th17 相关细胞因子(如白细胞介素(IL)- 17 和 IL-21)的产生来减少炎症反应。其他炎症细胞因子的水平也受到抑制,包括 IL-1β、IL-6 和肿瘤坏死因子 (TNF)-α。此外,研究还发现 EP 增加了 GDM 小鼠中 Tregs 的数量以及与 Treg 相关的细胞因子 IL-10 和转化生长因子-β TGF-β。总之,EP能调节小鼠的GDM,可能是治疗GDM患者的一种潜在治疗策略。
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引用次数: 0
Effects of MEK1/2 inhibitor U0126 on FGF10-enhanced buffalo oocyte maturation in vitro MEK1/2 抑制剂 U0126 对 FGF10 增强型水牛卵母细胞体外成熟的影响
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-20 DOI: 10.1016/j.repbio.2024.100883
Shanshan Du , Yanxin Wang , Fenghua Lu , Wenting Zhou

Fibroblast growth factor 10 (FGF10) plays critical roles in oocyte maturation and embryonic development; however, the specific pathway by which FGF10 promotes in vitro maturation of buffalo oocytes remains elusive. The present study was aimed at investigating the mechanism underlying effects of the FGF10-mediated extracellular regulated protein kinases (ERK) pathway on oocyte maturation and embryonic development in vitro. MEK1/2 (mitogen-activated protein kinase kinase) inhibitor U0126, alone or in combination with FGF10, was added to the maturation culture medium during maturation of the cumulus oocyte complex. Morphological observations, orcein staining, apoptosis detection, and quantitative real-time PCR were performed to evaluate oocyte maturation, embryonic development, and gene expression. U0126 affected oocyte maturation and embryonic development in vitro by substantially reducing the nuclear maturation of oocytes and expansion of the cumulus while increasing the apoptosis of cumulus cells. However, it did not have a considerable effect on glucose metabolism. These findings suggest that blocking the MEK/ERK pathway is detrimental to the maturation and embryonic development potential of buffalo oocytes. Overall, FGF10 may regulate the nuclear maturation of oocytes and cumulus cell expansion and apoptosis but not glucose metabolism through the MEK/ERK pathway. Our findings indicate that FGF10 regulates resumption of meiosis and expansion and survival of cumulus cells via MEK/ERK signaling during in vitro maturation of buffalo cumulus oocyte complexes. Elucidation of the mechanism of action of FGF10 and insights into oocyte maturation should advance buffalo breeding. Further studies should examine whether enhancement of MEK/ERK signaling improves embryonic development in buffalo.

成纤维细胞生长因子10(FGF10)在卵母细胞成熟和胚胎发育过程中发挥着关键作用;然而,FGF10促进水牛卵母细胞体外成熟的具体途径仍不清楚。本研究旨在探讨 FGF10 介导的细胞外调节蛋白激酶(ERK)通路对体外卵母细胞成熟和胚胎发育的影响机制。在积层卵母细胞复合体成熟过程中,在成熟培养液中单独或与 FGF10 联用 MEK1/2(丝裂原活化蛋白激酶激酶)抑制剂 U0126。对卵母细胞成熟、胚胎发育和基因表达进行了形态学观察、橙皮苷染色、细胞凋亡检测和实时定量 PCR 检测。U0126对体外卵母细胞成熟和胚胎发育有影响,它能显著降低卵母细胞的核成熟和积层的扩张,同时增加积层细胞的凋亡。然而,它对葡萄糖代谢的影响并不大。这些发现表明,阻断 MEK/ERK 通路不利于水牛卵母细胞的成熟和胚胎发育潜能。总之,FGF10可通过MEK/ERK途径调控卵母细胞核成熟、积聚细胞扩增和凋亡,但不能调控糖代谢。我们的研究结果表明,在水牛积核卵母细胞复合体体外成熟过程中,FGF10通过MEK/ERK信号传导调节减数分裂的恢复以及积核细胞的扩增和存活。阐明 FGF10 的作用机制并深入了解卵母细胞的成熟过程将促进水牛育种。进一步的研究应探讨 MEK/ERK 信号的增强是否能改善水牛的胚胎发育。
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引用次数: 0
The consequences of manipulating relaxin family peptide receptor 1 (RXFP1) level in ovarian cancer cells 操纵卵巢癌细胞中松弛素家族肽受体 1 (RXFP1) 水平的后果
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-19 DOI: 10.1016/j.repbio.2024.100864
Kamila Domińska , Kinga Anna Urbanek , Karolina Kowalska , Dominika Ewa Habrowska-Górczyńska , Marta Justyna Kozieł , Tomasz Ochędalski , Agnieszka Wanda Piastowska-Ciesielska

Deregulation of the relaxin family peptide system (RFPS) appears to increase the risk of range of cancers, including epithelial ovarian cancers (EOC). The present study examines the effect of relaxin family peptide receptor 1 (RXFP1) level on the biological properties of human epithelial ovarian adenocarcinoma cells (OVCAR4 and SKOV3). RXFP1 was downregulated (RXFP1↓) in the cells using the RXFP1 sgRNA CRISPR All-in-One Lentivirus set (pLenti-U6-sgRNA-SFFV-Cas9–2A-Puro), and upregulated (RXFP1↑) using the RXFP1 CRISPRa sgRNA Lentivector (pLenti-U6-sgRNA-PGK-Neo) kit, which activates the RXFP1 gene when paired with dCas9-SAM. The changes taking place during adhesion to extracellular matrix (ECM) proteins were assessed in multi-well plates coated with collagen, fibronectin, laminin and gelatin. Cellular viability was monitored based on mitochondrial metabolic activity (MTT Assay, Alamar Blue Assay) and adenosine triphosphate production (ATP Assay). The rate of cell proliferation was determined based on the percentage of Ki67 immunoreactive cells and the numbers of cells in particular cell-cycle phases. The mesenchymal-like (Boyden Chamber Assay) and amoeboid-like movements (Wound Healing Assay) of ovarian cancer cells were also analyzed after transfection. RXFP1 downregulation decreased the adhesion properties of ovarian cancer cells and increased the tendency for apoptosis under stressful conditions. In contrast, RXFP1 upregulation had pro-proliferative, pro-survival and promigratory effects. Our findings confirm that the relaxin-2/RXFP1 signaling pathway plays a role in the promotion of growth and progression of ovarian cancer.

弛缓素家族肽系统(RFPS)的失调似乎会增加包括上皮性卵巢癌(EOC)在内的一系列癌症的患病风险。本研究探讨了弛缓素家族肽受体 1(RXFP1)水平对人上皮性卵巢腺癌细胞(OVCAR4 和 SKOV3)生物学特性的影响。在使用 RXFP1 sgRNA CRISPR 一体化慢病毒组(pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro)的细胞中,RXFP1 被下调(RXFP1↓)、和 RXFP1 CRISPRa sgRNA Lentivector(pLenti-U6-sgRNA-PGK-Neo)试剂盒激活 RXFP1 基因(RXFP1↑)。在涂有胶原蛋白、纤连蛋白、层粘连蛋白和明胶的多孔板上评估了细胞粘附到细胞外基质(ECM)蛋白过程中发生的变化。根据线粒体代谢活性(MTT 检测法、阿拉玛蓝检测法)和三磷酸腺苷生成量(ATP 检测法)监测细胞活力。细胞增殖率是根据 Ki67 免疫反应细胞的百分比和处于特定细胞周期阶段的细胞数量确定的。还分析了转染后卵巢癌细胞的间质样(波伊登室试验)和变形样运动(伤口愈合试验)。下调 RXFP1 会降低卵巢癌细胞的粘附性,并增加其在应激条件下的凋亡倾向。相反,RXFP1 的上调具有促增殖、促存活和促生长的作用。我们的研究结果证实,弛缓素-2/RXFP1 信号通路在促进卵巢癌的生长和进展中发挥作用。
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引用次数: 0
The impact of estradiol supplementation on endometrial thickness and intrauterine insemination outcomes 补充雌二醇对子宫内膜厚度和宫腔内人工授精结果的影响
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-17 DOI: 10.1016/j.repbio.2024.100886
Wendy Y. Zhang , Megan McCracken , Lisandra Veliz Dominguez , Amy Zhang , Jasmyn Johal , Lusine Aghajanova

The impact of estrogen supplementation during the follicular/proliferative phase on the endometrial lining thickness (EMT) prior to intrauterine insemination (IUI) remains largely unstudied. Our study examined changes in EMT and rates of clinical pregnancy, miscarriage, and live birth for all patients who completed an IUI cycle at Stanford Fertility Center from 2017–2023 (n = 2281 cycles). Cycles with estradiol supplementation (n = 309) were compared to reference cycles without supplementation (n = 1972), with the reference cohort further categorized into cycles with a pre-ovulatory EMT of < 7 mm (“thin-lining”, n = 536) and ≥ 7 mm (“normal-lining”, n = 1436). The estradiol group had a statistically significant greater change in EMT from baseline to ovulation compared to the thin-lining reference groups (2.4 mm vs 1.9 mm, p < =0.0001). Similar rates of clinical pregnancy and live birth were observed. After adjusting for age, BMI, race/ethnicity, infertility diagnosis, and EMT at trigger, the estradiol cohort had a significantly increased odds of miscarriage versus the entire reference cohort (2.46, 95 % confidence interval [1.18, 5.14], p = 0.02). Thus, although estradiol supplementation had a statistically significant increase in EMT compared to IUI cycles with thin pre-ovulatory EMT (<7 mm), this change did not translate into improved IUI outcomes such as increased rates of clinical pregnancy and live birth or decreased rate of miscarriage. Our study suggests that supplemental estradiol does not appear to improve IUI outcomes.

在卵泡/增殖期补充雌激素对宫腔内人工授精(IUI)前子宫内膜厚度(EMT)的影响在很大程度上仍未得到研究。我们的研究考察了2017-2023年期间在斯坦福生育中心完成宫内人工授精周期的所有患者(n = 2281个周期)的EMT变化以及临床妊娠率、流产率和活产率。将补充雌二醇的周期(n = 309)与未补充雌二醇的参照周期(n = 1972)进行比较,参照队列进一步分为排卵前EMT为< 7 mm("薄衬",n = 536)和≥ 7 mm("正常衬",n = 1436)的周期。从基线到排卵期,雌二醇组的 EMT 变化比薄层参考组大,差异有统计学意义(2.4 mm vs 1.9 mm,p <=0.0001)。临床妊娠率和活产率相似。在对年龄、体重指数、种族/民族、不孕诊断和触发时的 EMT 进行调整后,雌二醇组的流产几率明显高于整个参照组(2.46,95% 置信区间 [1.18,5.14],p = 0.02)。因此,尽管与排卵前EMT较薄的人工授精周期(<7 mm)相比,补充雌二醇在统计学上显著增加了EMT,但这一变化并没有转化为人工授精结果的改善,如临床妊娠率和活产率的增加或流产率的降低。我们的研究表明,补充雌二醇似乎并不能改善人工授精的结果。
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引用次数: 0
Intrauterine infusion of platelet‐rich plasma improves fibrosis by transforming growth factor beta 1/Smad pathway in a rat intrauterine adhesion model 在大鼠宫内粘连模型中,宫腔内输注富血小板血浆可通过转化生长因子β1/Smad途径改善纤维化状况
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-10 DOI: 10.1016/j.repbio.2024.100882
Hongwen Yang , Wenmin Zhang , Jingchuan Fang , Zhihan Tang , Yanni Zhou , Hongzhen Hu , Miao miao , Yu Yao

This study aims to elucidate the effects of Platelet-rich plasma (PRP) in fibrosis development in intrauterine adhesion (IUA), and the associated underlying mechanisms are also explored, which are expected to be a potential therapeutic scheme for IUA. In this research, PRP was obtained and prepared from the peripheral venous blood of rats. A rat model was induced by mechanical injury. Further, PRP was directly injected into the uterus for treatment. The appearance and shape of the uterus were assessed based on the tissues harvested. The fibrosis biomarker levels were analyzed. The transforming growth factor beta 1 (TGF-β1) and Mothers against decapentaplegic homolog 7 (Smad7) levels, the phosphorylation of Smad2 (p-Smad2), and the phosphorylation of Smad3 (p-Smad3) were analyzed, and the molecular mechanism was investigated by rescue experiments. It was found that PRP improved the appearance and shape of the uterus in IUA and increased endometrial thickness and gland numbers. The administration of PRP resulted in a decrease in the expressions of fibrosis markers including collagen I, α-SMA, and fibronectin. Furthermore, PRP increased Smad7 levels and decreased TGF-β1 levels, p-Smad2, and p-Smad3. Meanwhile, administration of TGF-β1 activator reversed the therapeutic effects of PRP in IUA. Collectively, the intrauterine infusion of PRP can promote endometrial damage recovery and improve endometrial fibrosis via the TGF-β1/Smad pathway. Hence, PRP can be a potential therapeutic strategy for IUA.

本研究旨在阐明富血小板血浆(PRP)对宫腔内粘连(IUA)纤维化发展的影响,并探讨其相关的内在机制,有望成为 IUA 的潜在治疗方案。本研究从大鼠外周静脉血中获取并制备了 PRP。通过机械损伤诱导大鼠模型。然后,将 PRP 直接注入子宫进行治疗。根据采集的组织对子宫的外观和形状进行评估。对纤维化生物标志物水平进行了分析。分析了转化生长因子β1(TGF-β1)和母亲抗截瘫同源物7(Smad7)的水平、Smad2的磷酸化(p-Smad2)和Smad3的磷酸化(p-Smad3),并通过挽救实验研究了其分子机制。结果发现,PRP能改善IUA子宫的外观和形态,增加子宫内膜厚度和腺体数量。给予 PRP 后,纤维化标记物(包括胶原 I、α-SMA 和纤维连接蛋白)的表达量减少。此外,PRP 提高了 Smad7 的水平,降低了 TGF-β1 水平、p-Smad2 和 p-Smad3。同时,给予 TGF-β1 激活剂可逆转 PRP 对 IUA 的治疗效果。综上所述,PRP宫腔内灌注可通过TGF-β1/Smad途径促进子宫内膜损伤恢复并改善子宫内膜纤维化。因此,PRP可作为IUA的一种潜在治疗策略。
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引用次数: 0
Influence of female sex hormones on proactive behavioral and physiological immune parameters 女性性激素对主动行为和生理免疫参数的影响
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-05 DOI: 10.1016/j.repbio.2024.100880
Judith K. Keller, Esther K. Diekhof

Women may be more susceptible to infections in the luteal phase, supposedly as a consequence of the hormone progesterone and its immunosuppressive action. While immunosuppression may be important for successful oocyte implantation and pregnancy, it makes women more vulnerable to pathogens. According to theory, to compensate for reduced immunocompetence, women in the luteal phase exhibit proactive behavioral responses, such as disgust and avoidance of disease-associated stimuli, to minimize contagion risk. However, previous studies yielded inconsistent results, and did not account for accompanying proactive immune responses, like the increase of secretory immunoglobin A (sIgA). Here, we assessed the proactive immune response and feelings of disgust associated with disease cues in the comparison of 61 woman with a natural menstrual cycle (31 in the follicular and 30 in the luteal phase) and 20 women taking hormonal contraception (HC). Women rated disease vulnerability and disgust propensity, watched a video displaying people with respiratory symptoms, which was evaluated for its disgust-evoking potential and contagiousness, and provided saliva samples for hormone and sIgA analysis. Women with HC reported a heightened vulnerability to disease compared to naturally cycling women, whereas both the feeling of disgust and the sIgA increase elicited by the disease video were similar across groups, regardless of progesterone. We found a u-shaped relationship between progesterone and baseline sIgA in naturally cycling women, with its nadir during ovulation. Overall, our data do not support a compensatory relationship between the proposed progesterone-induced immunosuppression and heightened disgust or a proactive sIgA response.

妇女在黄体期可能更容易受到感染,这可能是激素孕酮及其免疫抑制作用的结果。虽然免疫抑制对卵母细胞的成功着床和怀孕可能很重要,但它也使女性更容易受到病原体的感染。根据理论,为了弥补免疫能力的下降,处于黄体期的妇女会表现出积极的行为反应,如厌恶和回避与疾病相关的刺激,以最大限度地降低传染风险。然而,以往的研究结果并不一致,也没有考虑到伴随的主动免疫反应,如分泌型免疫球蛋白 A(sIgA)的增加。在此,我们对 61 名自然月经周期的女性(31 名处于卵泡期,30 名处于黄体期)和 20 名服用激素避孕药(HC)的女性进行了对比,评估了与疾病线索相关的主动免疫反应和厌恶感。妇女们对疾病易感性和厌恶倾向进行了评分,观看了一段显示呼吸道症状患者的视频,并对其引发厌恶的可能性和传染性进行了评估,她们还提供了唾液样本以进行激素和 sIgA 分析。与自然周期的妇女相比,HC 妇女对疾病的易感性更高,而疾病视频所引起的厌恶感和 sIgA 升高在各组间相似,与孕酮无关。我们发现,自然周期妇女的孕酮与基线 sIgA 之间呈 "u "形关系,在排卵期达到最低点。总之,我们的数据并不支持孕酮诱导的免疫抑制与厌恶感增强或主动的 sIgA 反应之间的补偿关系。
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引用次数: 0
IVF outcomes pre- and post-anti-COVID-19 vaccination – Are there any differences? 接种抗-COVID-19 疫苗前后的试管婴儿结果--是否存在差异?
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-27 DOI: 10.1016/j.repbio.2024.100879
Marina Arselia Fradkin , Heli Alexandroni , Talia Eldar-Geva , Ido Ben-Ami

Since the beginning of the SARS-CoV-2 pandemic, there have been rising concerns about the virus's possible ability to affect male and female fertility. Although effective vaccines were introduced and the vaccination rate of the general population is high, some reproductive-age individuals are still hesitant to receive the vaccine, because of an unestablished belief that the vaccine might impair fertility. In this single-center retrospective study, encompassing data from 387 medical files of in-vitro fertilization (IVF) patients we compared IVF cycle outcomes and sperm characteristics in vaccinated couples before and after vaccination, as well as between vaccinated patients and a control group of individuals who were neither vaccinated nor infected with COVID-19 before or during the cycles. We found no significant differences between vaccinated and non-vaccinated patients concerning the number of retrieved oocytes and the total motile sperm count (TMC). The mean number of retrieved oocytes showed a slight increase in the vaccinated group compared to the non-vaccinated control group (10.8 vs. 9.18, p = 0.14). Additionally, within the vaccinated group, no significant difference was observed in the mean number of oocytes before and after vaccination (9.7 and 10.8, p = 0.14). Other similar cycle outcomes between the groups were the rates of implantation, pregnancy, and ovarian hyperstimulation syndrome. This study emphasized that the mRNA anti-COVID-19 vaccination doesn’t adversely affect ovarian response or sperm quality in IVF patients. These findings contribute valuable insights to the safety profile of anti-COVID-19 vaccines in the context of reproductive-aged populations, aiding decision-making during ongoing virus outbreaks and potential future scenarios.

自 SARS-CoV-2 大流行以来,人们越来越担心该病毒可能会影响男性和女性的生育能力。虽然有效的疫苗已经问世,普通人群的疫苗接种率也很高,但一些育龄人群仍对接种疫苗犹豫不决,因为他们认为疫苗可能会影响生育能力。在这项单中心回顾性研究中,我们比较了接种疫苗前后接种疫苗夫妇的试管受精周期结果和精子特征,以及接种疫苗患者和对照组(既未接种疫苗,也未在接种前或接种期间感染 COVID-19)之间的差异。我们发现,接种疫苗和未接种疫苗的患者在取回的卵母细胞数和总活动精子数(TMC)方面没有明显差异。与未接种疫苗的对照组相比,接种疫苗组获得的卵母细胞平均数量略有增加(10.8 对 9.18,P = 0.14)。此外,在接种疫苗组中,接种疫苗前后的平均卵母细胞数没有明显差异(9.7 和 10.8,p = 0.14)。两组之间其他相似的周期结果包括植入率、妊娠率和卵巢过度刺激综合征。这项研究强调,mRNA抗COVID-19疫苗接种不会对试管婴儿患者的卵巢反应或精子质量产生不利影响。这些研究结果对育龄人群接种抗COVID-19疫苗的安全性提出了宝贵的见解,有助于在病毒爆发期间和未来可能发生的情况下做出决策。
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引用次数: 0
Association of maternal blood high-mobility group box 1 levels and adverse pregnancy outcomes: A systematic review and meta-analysis 孕产妇血液中高流动性组方框 1 水平与不良妊娠结局的关系:系统回顾和荟萃分析
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-15 DOI: 10.1016/j.repbio.2024.100859
Liping Xue , Ruolin Chen , Ying Liu , Peiguang Niu , Jintuo Zhou , Jinhua Liu , Jinhua Zhang , Huajiao Chen

Conflicting findings have emerged regarding the levels of high mobility group box 1 (HMGB1) in individuals experiencing adverse pregnancy outcomes. Here we conducted a meta-analysis to assess the association between maternal blood HMGB1 levels and adverse pregnancy outcomes. Utilizing databases such as PubMed, Cochrane Central Register of Controlled Trials, Web of Science, Embase and China National Knowledge Infrastructure (CNKI), a systematic literature search was conducted in January 2024. Eligible literature was screened according to inclusion and exclusion criteria. Quality assessment was evaluated using the Newcastle-Ottawa Scale (NOS). The extracted data were analyzed using Review Manager 5.4 and STATA 12.0 software. 21 observational studies with a total of 2471 participants were included in this meta-analysis. Significantly higher peripheral blood levels of HMGB1 were associated with preeclampsia (PE) (SMD=1.34; 95% CI: 0.72–1.95; P < 0.0001) and gestational diabetes mellitus (GDM) (SMD=1.20; 95% CI: 0.31–2.09; P = 0.009). Additionally, HMGB1 levels in peripheral blood were significantly elevated in patients with unexplained recurrent spontaneous abortion (URSA) than those in pregnancy controls (SMD=4.22; 95% CI: 1.64–6.80; P = 0.001) or non-pregnancy controls (SMD=3.87; 95% CI: 1.81–5.92; P = 0.0002). Interestingly, higher blood HMGB1 levels were observed in women with preterm birth (PTB), however, the results did not reach a statistical difference (SMD=0.54; 95% CI: −0.36–1.44; P = 0.24). In conclusion, overexpressed maternal blood HMGB1 levels were associated with adverse pregnancy outcomes, including PE, GDM and URSA. Further studies should be conducted to validate the efficacy of HMGB1 as a biomarker for assessing the risk of adverse pregnancy outcomes.

关于不良妊娠结局患者体内高迁移率组框 1(HMGB1)水平的研究结果存在冲突。在此,我们进行了一项荟萃分析,以评估母体血液中 HMGB1 水平与不良妊娠结局之间的关联。我们于 2024 年 1 月利用 PubMed、Cochrane Central Register of Controlled Trials、Web of Science、Embase 和中国国家知识基础设施(CNKI)等数据库进行了系统性文献检索。根据纳入和排除标准对符合条件的文献进行了筛选。采用纽卡斯尔-渥太华量表(NOS)进行质量评估。提取的数据使用 Review Manager 5.4 和 STATA 12.0 软件进行分析。本次荟萃分析共纳入了 21 项观察性研究,共有 2471 人参与。外周血中 HMGB1 水平显著升高与子痫前期(PE)(SMD=1.34;95% CI:0.72-1.95;P < 0.0001)和妊娠糖尿病(GDM)(SMD=1.20;95% CI:0.31-2.09;P = 0.009)有关。此外,与妊娠对照组(SMD=4.22;95% CI:1.64-6.80;P = 0.001)或非妊娠对照组(SMD=3.87;95% CI:1.81-5.92;P = 0.0002)相比,不明原因复发性自然流产(URSA)患者外周血中的 HMGB1 水平显著升高。有趣的是,在早产(PTB)妇女中观察到较高的血液 HMGB1 水平,但结果未达到统计学差异(SMD=0.54;95% CI:-0.36-1.44;P = 0.24)。总之,母体血液中过表达的 HMGB1 水平与不良妊娠结局有关,包括 PE、GDM 和 URSA。应开展进一步的研究来验证 HMGB1 作为评估不良妊娠结局风险的生物标志物的有效性。
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引用次数: 0
Androgens and Notch signaling cooperate in seminiferous epithelium to regulate genes related to germ cell development and apoptosis 雄激素和 Notch 信号在曲细精管上皮细胞中合作调节与生殖细胞发育和凋亡有关的基因
IF 2.1 3区 生物学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-15 DOI: 10.1016/j.repbio.2024.100878
Alicja Kamińska , Sylwia Lustofin , Małgorzata Brzoskwinia , Michał Duliban , Joanna Cyran-Gryboś , Barbara Bilińska , Anna Hejmej

It was reported previously that in adult males disruption of both androgen and Notch signaling impairs spermatid development and germ cell survival in rodent seminiferous epithelium. To explain the molecular mechanisms of these effects, we focused on the interaction between Notch signaling and androgen receptor (AR) in Sertoli cells and investigate its role in the control of proteins involved in apical ectoplasmic specializations, actin remodeling during spermiogenesis, and induction of germ cell apoptosis. First, it was revealed that in rat testicular explants ex vivo both testosterone and Notch signaling modulate AR expression and cooperate in the regulation of spermiogenesis-related genes (Nectin2, Afdn, Arp2, Eps8) and apoptosis-related genes (Fasl, Fas, Bax, Bcl2). Further, altered expression of these genes was found following exposure of Sertoli cells (TM4 cell line) and germ cells (GC-2 cell line) to ligands for Notch receptors (Delta-like1, Delta-like4, and Jagged1) and/or Notch pathway inhibition. Finally, direct interactions of Notch effector, Hairy/enhancer-of-split related with YRPW motif protein 1, and the promoter of Ar gene or AR protein were revealed in TM4 Sertoli cells. In conclusion, Notch pathway activity in Sertoli and germ cells regulates genes related to germ cell development and apoptosis acting both directly and indirectly by influencing androgen signaling in Sertoli cells.

以前曾有报道称,在成年雄性啮齿类动物的曲细精管上皮细胞中,雄激素和Notch信号的中断会影响精子的发育和生殖细胞的存活。为了解释这些影响的分子机制,我们重点研究了Sertoli细胞中Notch信号与雄激素受体(AR)之间的相互作用,并研究了其在控制参与顶端外质特化、精子形成过程中肌动蛋白重塑以及诱导生殖细胞凋亡的蛋白质中的作用。首先,研究发现,在大鼠睾丸外植体中,睾酮和Notch信号都会调节AR的表达,并合作调控精子发生相关基因(Nectin2、Afdn、Arp2、Eps8)和凋亡相关基因(Fasl、Fas、Bax、Bcl2)。此外,在将 Sertoli 细胞(TM4 细胞系)和生殖细胞(GC-2 细胞系)暴露于 Notch 受体配体(Delta-like1、Delta-like4 和 Jagged1)和/或 Notch 通路抑制剂后,发现这些基因的表达发生了改变。最后,在TM4 Sertoli细胞中发现了Notch效应因子--与YRPW基调蛋白1相关的毛发/分裂增强子--与Ar基因或AR蛋白启动子的直接相互作用。总之,Notch通路在Sertoli细胞和生殖细胞中的活性通过影响Sertoli细胞中的雄激素信号,直接或间接地调控与生殖细胞发育和凋亡有关的基因。
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引用次数: 0
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Reproductive biology
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