首页 > 最新文献

Recent patents on biotechnology最新文献

英文 中文
Exploration of Developmental Variants of Predatory Ladybird, Coccinella septumpunctata L. (Coleoptera: Coccinellidae) on an Artificial Diet. 捕食性瓢虫 Coccinella septumpunctata L.(鞘翅目:瓢虫科)在人工食物上的发育变异探索
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-01-01 DOI: 10.2174/0118722083313758240813102715
Arshi Siddiqui, Shaizee, Danish Khan

Introduction: This study aimed to focus on the identification, rearing, and exploration of developmental variants of the predatory ladybird, Coccinella septempunctata L., renowned for its efficacy as a biological control agent and its predation on agricultural pests. However, comprehensive knowledge concerning the occurrence and characteristics of developmental variants in this species remains limited.

Methods: In this study, through meticulous monitoring and exploration, we identified developmental variants exhibiting distinct sexual attributes, as well as survival rates.

Results: The research outcomes enhance our understanding of the developmental variations within an egg batch of C. septempunctata.

Conclusion: Moreover, the findings hold practical implications for the implementation of biological control strategies in agriculture, as specific variants may possess unique characteristics that enhance their effectiveness as natural enemies against pests. Furthermore, the increasing competitiveness in the artificial diet space for scientific models raises questions about intellectual property rights (IPR), patents, and strategies. This overview looks at recent developments and advanced protection strategies in this field to help understand the present state of IPR and patents in an artificial food for insects.

导言:该研究的目的是鉴定、饲养和探索捕食性瓢虫七星瓢虫的发育变体。然而,有关该物种发育变体的发生和特征的全面知识仍然有限:方法:在本研究中,通过细致的监测和探索,我们确定了表现出不同性状的发育变体以及存活率:结果:研究成果加深了我们对七鳃鳗一批卵内发育变异的了解:此外,研究结果对在农业中实施生物防治战略具有实际意义,因为特定变体可能具有独特的特征,能提高其作为天敌对付害虫的效果。
{"title":"Exploration of Developmental Variants of Predatory Ladybird, <i>Coccinella septumpunctata</i> L. (Coleoptera: Coccinellidae) on an Artificial Diet.","authors":"Arshi Siddiqui, Shaizee, Danish Khan","doi":"10.2174/0118722083313758240813102715","DOIUrl":"10.2174/0118722083313758240813102715","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to focus on the identification, rearing, and exploration of developmental variants of the predatory ladybird, <i>Coccinella septempunctata</i> L., renowned for its efficacy as a biological control agent and its predation on agricultural pests. However, comprehensive knowledge concerning the occurrence and characteristics of developmental variants in this species remains limited.</p><p><strong>Methods: </strong>In this study, through meticulous monitoring and exploration, we identified developmental variants exhibiting distinct sexual attributes, as well as survival rates.</p><p><strong>Results: </strong>The research outcomes enhance our understanding of the developmental variations within an egg batch of <i>C. septempunctata</i>.</p><p><strong>Conclusion: </strong>Moreover, the findings hold practical implications for the implementation of biological control strategies in agriculture, as specific variants may possess unique characteristics that enhance their effectiveness as natural enemies against pests. Furthermore, the increasing competitiveness in the artificial diet space for scientific models raises questions about intellectual property rights (IPR), patents, and strategies. This overview looks at recent developments and advanced protection strategies in this field to help understand the present state of IPR and patents in an artificial food for insects.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":"244-250"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Emerging Methods in the Identification of Bacterial Respiratory Tract Pathogens. 鉴定呼吸道细菌病原体的新方法。
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-01-01 DOI: 10.2174/0118722083309510240625085931
Flora Kiaghobadi, Ali Dehshahri, Younes Ghasemi, Mohammad Hossein Morowvat

Aims: Here, we will review different bacterial causes of respiratory tract infections and discuss the available diagnostic methods. Moreover, we will provide some recently published patents and newer techniques, such as respiratory panels and omics approaches, and express the challenges in this path.

Background: Respiratory tract infections (RTIs) include those infections that can lead to the involvement of different respiratory parts, including the sinuses, throat, airways, and lungs. Acute respiratory tract infection is the leading cause of death from infectious illnesses worldwide. According to the World Health Organization, 1.6 to 2.2 million deaths have occurred due to acute respiratory infections in children under five years of age. About 4 million people die annually from respiratory infections, 98% of which are caused by lower respiratory infections.

Results: Depending on the type of pathogen, the severity of the infection can vary from mild to severe and even cause death. The most important pathogens involved in respiratory tract infections include Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. The symptoms are often similar, but the treatment can vary greatly. Therefore, correct diagnosis is so important. There are several methods for diagnosing respiratory infections. Traditional tests include the culture of respiratory samples, considered the primary tool for diagnosing respiratory infections in laboratories, and less common standard tests include rapid and antigenic tests. It is essential to think that the culture method is reliable. In the original method of diagnosing respiratory infections, some bacteria were challenging to grow successfully, and many clinical laboratories needed to be equipped for viral cultures. Another issue is the time to get the results, which may take up to 7 days. Rapid and antigenic tests are faster but need to be more accurate.

Conclusion: The clinical laboratories are trying to be equipped with molecular methods for detecting respiratory pathogens and identifying the genetic material of the infectious agent in these new methods as the primary method in their agenda.

目的:在此,我们将回顾导致呼吸道感染的不同细菌病因,并讨论现有的诊断方法。此外,我们还将提供一些最新发表的专利和较新的技术,如呼吸系统面板和 omics 方法,并阐述这一领域所面临的挑战:背景:呼吸道感染(RTIs)包括可导致不同呼吸道部位受累的感染,包括鼻窦、咽喉、呼吸道和肺部。急性呼吸道感染是全球传染病致死的主要原因。据世界卫生组织统计,五岁以下儿童因急性呼吸道感染死亡的人数达 160 万至 220 万。每年约有 400 万人死于呼吸道感染,其中 98% 由下呼吸道感染引起:根据病原体的种类,感染的严重程度可从轻微到严重不等,甚至会导致死亡。呼吸道感染最主要的病原体包括肺炎链球菌、流感嗜血杆菌和白喉摩拉菌。症状通常相似,但治疗方法却大相径庭。因此,正确诊断非常重要。诊断呼吸道感染有多种方法。传统的检测方法包括呼吸道样本培养,这被认为是实验室诊断呼吸道感染的主要工具,而不太常见的标准检测方法包括快速检测和抗原检测。必须认为培养方法是可靠的。在最初的呼吸道感染诊断方法中,一些细菌的成功培养具有挑战性,许多临床实验室需要配备病毒培养设备。另一个问题是得到结果的时间,可能需要长达 7 天的时间。快速和抗原检测更快,但需要更准确:结论:临床实验室正试图配备检测呼吸道病原体的分子方法,并将这些新方法作为主要方法来鉴定传染源的遗传物质。
{"title":"Emerging Methods in the Identification of Bacterial Respiratory Tract Pathogens.","authors":"Flora Kiaghobadi, Ali Dehshahri, Younes Ghasemi, Mohammad Hossein Morowvat","doi":"10.2174/0118722083309510240625085931","DOIUrl":"10.2174/0118722083309510240625085931","url":null,"abstract":"<p><strong>Aims: </strong>Here, we will review different bacterial causes of respiratory tract infections and discuss the available diagnostic methods. Moreover, we will provide some recently published patents and newer techniques, such as respiratory panels and omics approaches, and express the challenges in this path.</p><p><strong>Background: </strong>Respiratory tract infections (RTIs) include those infections that can lead to the involvement of different respiratory parts, including the sinuses, throat, airways, and lungs. Acute respiratory tract infection is the leading cause of death from infectious illnesses worldwide. According to the World Health Organization, 1.6 to 2.2 million deaths have occurred due to acute respiratory infections in children under five years of age. About 4 million people die annually from respiratory infections, 98% of which are caused by lower respiratory infections.</p><p><strong>Results: </strong>Depending on the type of pathogen, the severity of the infection can vary from mild to severe and even cause death. The most important pathogens involved in respiratory tract infections include <i>Streptococcus pneumoniae, Haemophilus influenzae</i>, and <i>Moraxella catarrhalis</i>. The symptoms are often similar, but the treatment can vary greatly. Therefore, correct diagnosis is so important. There are several methods for diagnosing respiratory infections. Traditional tests include the culture of respiratory samples, considered the primary tool for diagnosing respiratory infections in laboratories, and less common standard tests include rapid and antigenic tests. It is essential to think that the culture method is reliable. In the original method of diagnosing respiratory infections, some bacteria were challenging to grow successfully, and many clinical laboratories needed to be equipped for viral cultures. Another issue is the time to get the results, which may take up to 7 days. Rapid and antigenic tests are faster but need to be more accurate.</p><p><strong>Conclusion: </strong>The clinical laboratories are trying to be equipped with molecular methods for detecting respiratory pathogens and identifying the genetic material of the infectious agent in these new methods as the primary method in their agenda.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":"198-209"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141535155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Edible Vaccines: A Patent-Driven Exploration of Immunization Technologies. 食用疫苗:专利驱动的免疫技术探索。
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-01-01 DOI: 10.2174/0118722083275041231219060829
Sahil Kashyap, Rohit Kamboj, Shikha Kamboj, Kumar Guarve, Sweta Kamboj

Vaccines are biological preparations that improve immunity to particular diseases. Particularly for poor developing nations, edible vaccines show significant potential as a financially advantageous, simple to administer, straightforward to store, fail-safe, and socially and culturally acceptable vaccine delivery system. A vaccine incorporates the gene-encoding bacterial or viral disease-causing agent in plants without losing its immunogenic property. Potatoes, tomatoes, rice, soybeans, and bananas are the primary plants for edible vaccines. It activates the systemic and mucosal immunity responses against a foreign disease-causing organism. It offers exciting possibilities to reduce diseases like hepatitis B, rabies, HIV/AIDS (human immunodeficiency virus infection and acquired immune deficiency syndrome), etc. These vaccines provide many benefits, like being convenient to administer, efficiently storing, and readily acceptable drug delivery systems for patients of different age groups. So, an edible vaccine may be the most convenient vaccine to improve immunity. However, there are a lot of technical and regulatory challenges to overcome in the way of edible vaccine technology. Though all seem surmountable, various technical obstacles and regulatory and non-scientific challenges need to be overcome. Moreover, edible vaccine patents represent a cutting-edge area of biotechnology, where the integration of genetic material into edible substances holds great promise for revolutionizing vaccination methods. These patents aim to harness the potential of plants and other edibles to stimulate immune responses, offering a potential alternative to traditional injectable vaccines. This review states the technologies, host plants, current status, recent patents, the future of this new preventive modality, and different regulatory issues concerning edible vaccines.

疫苗是一种生物制剂,可提高对特定疾病的免疫力。特别是对于贫穷的发展中国家来说,可食用疫苗作为一种经济上有利、管理简单、易于储存、安全可靠以及社会和文化上可接受的疫苗输送系统,显示出巨大的潜力。疫苗将编码细菌或病毒致病因子的基因植入植物中,而不会失去其免疫原性。马铃薯、西红柿、大米、大豆和香蕉是可食用疫苗的主要植物。它能激活全身和粘膜免疫反应,抵御外来致病生物。它为减少乙型肝炎、狂犬病、艾滋病毒/艾滋病(人类免疫缺陷病毒感染和获得性免疫缺陷综合征)等疾病提供了令人兴奋的可能性。这些疫苗有很多好处,比如方便接种、有效储存,以及适合不同年龄段患者的给药系统。因此,可食用疫苗可能是提高免疫力最方便的疫苗。然而,在可食用疫苗技术的发展道路上,还有许多技术和监管方面的挑战需要克服。尽管所有这些似乎都可以克服,但各种技术障碍以及监管和非科学挑战仍需克服。此外,可食用疫苗专利代表了生物技术的一个前沿领域,将遗传物质融入可食用物质有望彻底改变疫苗接种方法。这些专利旨在利用植物和其他可食用物质的潜力来刺激免疫反应,为传统注射疫苗提供潜在的替代方案。本综述介绍了可食用疫苗的技术、宿主植物、现状、最新专利、这一新型预防方式的未来,以及与可食用疫苗有关的各种监管问题。
{"title":"Edible Vaccines: A Patent-Driven Exploration of Immunization Technologies.","authors":"Sahil Kashyap, Rohit Kamboj, Shikha Kamboj, Kumar Guarve, Sweta Kamboj","doi":"10.2174/0118722083275041231219060829","DOIUrl":"10.2174/0118722083275041231219060829","url":null,"abstract":"<p><p>Vaccines are biological preparations that improve immunity to particular diseases. Particularly for poor developing nations, edible vaccines show significant potential as a financially advantageous, simple to administer, straightforward to store, fail-safe, and socially and culturally acceptable vaccine delivery system. A vaccine incorporates the gene-encoding bacterial or viral disease-causing agent in plants without losing its immunogenic property. Potatoes, tomatoes, rice, soybeans, and bananas are the primary plants for edible vaccines. It activates the systemic and mucosal immunity responses against a foreign disease-causing organism. It offers exciting possibilities to reduce diseases like hepatitis B, rabies, HIV/AIDS (human immunodeficiency virus infection and acquired immune deficiency syndrome), etc. These vaccines provide many benefits, like being convenient to administer, efficiently storing, and readily acceptable drug delivery systems for patients of different age groups. So, an edible vaccine may be the most convenient vaccine to improve immunity. However, there are a lot of technical and regulatory challenges to overcome in the way of edible vaccine technology. Though all seem surmountable, various technical obstacles and regulatory and non-scientific challenges need to be overcome. Moreover, edible vaccine patents represent a cutting-edge area of biotechnology, where the integration of genetic material into edible substances holds great promise for revolutionizing vaccination methods. These patents aim to harness the potential of plants and other edibles to stimulate immune responses, offering a potential alternative to traditional injectable vaccines. This review states the technologies, host plants, current status, recent patents, the future of this new preventive modality, and different regulatory issues concerning edible vaccines.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":"99-112"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139567388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploration of Polyhydroxybutyrate (PHB) Production Potential of Photosynthetic Microbes: A Sustainable Source of Bioplastic. 光合微生物生产聚羟基丁酸盐(PHB)潜力的探索:生物塑料的可持续来源。
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-12-31 DOI: 10.2174/0118722083295143241220054012
Saima Shahzad Mirza, Kanza Yaseen, Sumaira Aslam, Ammara Fatima, Mohammad Hossein Morowvat, Ayesha Ishaque, Kalsoom Fatima

Introduction: The present study examined Polyhydroxy butyrate production (PHB) potential of different photosynthetic microbes such as Chlorella vulgaris, Scenedesmus obliquus and Rhodobacter capsulatus-PK under different nutrient conditions. Biodegradable bioplastics, such as Poly-β-hydroxybutyrates (PHB), derived from these microbes provide a sustainable alternative to conventional petroleum-based nondegradable plastics.

Background: As the demand for clean and sustainable alternatives rises, bio-plastic is gaining attention as a viable substitute to conventional plastics. However, conventional sources of bio-plastic production have inherent limitations, which can be effectively addressed through the utilization of photosynthetic microbes e.g. microalgae, purple non sulphur bacteria.

Methodology: The production of bioplastic was evaluated by cultivating the microalgae in BG-11, BBM and PNSB in synthetic growth media (MI, MII) with different nitrogen concentrations of 0%, 50% and 100%. The biopolymer (PHB) was obtained from all experiments in a wide range of concentration (7-42.8%) of dry cell weight (DCW).

Results: In this study, algal isolate SK1 demonstrated the highest PHB content (42.8%) in BBM under 100% nitrogen starvations rendering the bioplastic exceptionally compatible and suitable for eco-friendly applications. Additionally, various patents cited by different authors on different aspects of microbial bioplastic production.

Conclusion: Nutrition depletion such as nitrogen scarcity induced stressful growth conditions that resulted in highest accumulation of the biopolymer PHB. Optimizing nitrogen availability is key to maximizing PHB production, making it a viable sustainable alternative to conventional plastics.

引言:本研究考察了在不同营养条件下不同光合微生物(如小球藻、钝顶藻和荚膜杆菌-PK)生产聚羟基丁酸盐(PHB)的潜力。从这些微生物中提取的聚-β-羟基丁酸盐(PHB)等可生物降解的生物塑料为传统的石油基不可降解塑料提供了一种可持续的替代品:背景:随着人们对清洁和可持续替代品需求的增加,生物塑料作为传统塑料的可行替代品日益受到关注。然而,传统的生物塑料生产来源有其固有的局限性,而利用光合微生物(如微藻、紫色无硫细菌)可以有效地解决这些问题:通过在合成生长培养基(MI、MII)(氮浓度分别为 0%、50% 和 100%)中培养 BG-11、BBM 和 PNSB 微藻,对生物塑料的生产进行了评估。所有实验都获得了生物聚合物(PHB),浓度范围为干细胞重量(DCW)的 7-42.8%:结果:在这项研究中,藻类分离物 SK1 在 100%氮饥饿条件下的 BBM 中显示出最高的 PHB 含量(42.8%),使生物塑料具有极高的兼容性,适合生态友好型应用。此外,不同作者就微生物生物塑料生产的不同方面引用了多项专利:结论:营养枯竭(如氮稀缺)诱发了应激生长条件,导致生物聚合物 PHB 的最高积累。优化氮的可用性是最大限度提高 PHB 产量的关键,使其成为传统塑料的一种可行的可持续替代品。
{"title":"Exploration of Polyhydroxybutyrate (PHB) Production Potential of Photosynthetic Microbes: A Sustainable Source of Bioplastic.","authors":"Saima Shahzad Mirza, Kanza Yaseen, Sumaira Aslam, Ammara Fatima, Mohammad Hossein Morowvat, Ayesha Ishaque, Kalsoom Fatima","doi":"10.2174/0118722083295143241220054012","DOIUrl":"https://doi.org/10.2174/0118722083295143241220054012","url":null,"abstract":"<p><strong>Introduction: </strong>The present study examined Polyhydroxy butyrate production (PHB) potential of different photosynthetic microbes such as Chlorella vulgaris, Scenedesmus obliquus and Rhodobacter capsulatus-PK under different nutrient conditions. Biodegradable bioplastics, such as Poly-β-hydroxybutyrates (PHB), derived from these microbes provide a sustainable alternative to conventional petroleum-based nondegradable plastics.</p><p><strong>Background: </strong>As the demand for clean and sustainable alternatives rises, bio-plastic is gaining attention as a viable substitute to conventional plastics. However, conventional sources of bio-plastic production have inherent limitations, which can be effectively addressed through the utilization of photosynthetic microbes e.g. microalgae, purple non sulphur bacteria.</p><p><strong>Methodology: </strong>The production of bioplastic was evaluated by cultivating the microalgae in BG-11, BBM and PNSB in synthetic growth media (MI, MII) with different nitrogen concentrations of 0%, 50% and 100%. The biopolymer (PHB) was obtained from all experiments in a wide range of concentration (7-42.8%) of dry cell weight (DCW).</p><p><strong>Results: </strong>In this study, algal isolate SK1 demonstrated the highest PHB content (42.8%) in BBM under 100% nitrogen starvations rendering the bioplastic exceptionally compatible and suitable for eco-friendly applications. Additionally, various patents cited by different authors on different aspects of microbial bioplastic production.</p><p><strong>Conclusion: </strong>Nutrition depletion such as nitrogen scarcity induced stressful growth conditions that resulted in highest accumulation of the biopolymer PHB. Optimizing nitrogen availability is key to maximizing PHB production, making it a viable sustainable alternative to conventional plastics.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Green-Synthesized Nanoparticles on Dental Caries-Causing Bacteria: A Systematic Review. 绿色合成纳米颗粒对致龋细菌的影响:系统综述。
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-12-18 DOI: 10.2174/0118722083343723241204053839
Asma Sepahdar, Behnoush Selahbarzin, Atoosa Vaez Naini, Samira Jafari, Mehrnaz Moradi, Pegah Shakib
<p><strong>Introduction: </strong>In this study, a comprehensive search was conducted across multiple databases, including PubMed, Google Scholar, SID, Scopus, Medline, and Web of Science, as well as selected herbal medicine journals. The inclusion of herbal medicine journals was justified by the growing interest in green-synthesized nanoparticles derived from plant extracts, which are relevant to the focus of this review. Articles published between 2015 and 2023 were evaluated. To ensure a robust selection process, studies were assessed for quality using specific criteria beyond the AXIS checklist, including study design, sample size, and methodological rigor. Any discrepancies between reviewers were resolved through discussion or consultation with a third reviewer. This approach aimed to minimize bias and ensure the inclusion of high-quality studies that contribute meaningfully to the review's objectives.</p><p><strong>Objective: </strong>The primary objective of this review was to investigate the therapeutic implications of green-synthesized metallic nanoparticles, such as silver and zinc nanoparticles, in addressing dental caries, with a focus on their antibacterial properties and potential to improve current treatment modalities.</p><p><strong>Materials and methods: </strong>In this study, PubMed, Google Scholar, SID, Scopus, Medline, Web of Science, and herbal medicine journals were searched, and articles from 2015 to 2023 were evaluated.</p><p><strong>Results: </strong>In this study, 22 clinical trials were reviewed, and the effectiveness of synthesized nanoparticles was demonstrated in nearly all of them. The nanoparticles found to be most effective against tooth decay-causing bacteria include silver, zinc, selenium, nickel, and copper nanoparticles. To provide greater clarity, key findings, such as nanoparticle size, minimum inhibitory concentration (MIC) values, and specific antibacterial effects, are presented in a table. Additionally, a more in-depth comparison was made between these nanoparticles, focusing on their relative effectiveness under different conditions. For example, silver nanoparticles were consistently effective across various trials due to their strong antimicrobial properties, while zinc and copper nanoparticles showed efficacy in specific concentrations and against particular bacterial strains. Furthermore, green-synthesized nanoparticles were compared with those produced using traditional methods, evaluating factors, such as effectiveness, scalability, and safety, to provide a comprehensive understanding of their potential in dental caries treatment.</p><p><strong>Conclusion: </strong>According to the literature review, nanoparticles with antibacterial properties have the potential to serve as an alternative or complement to conventional chemical treatments for combating tooth decay. Future research should focus on exploring specific types of nanoparticles, such as green-synthesized metallic nanoparticles (e.g., sil
本研究在PubMed、谷歌Scholar、SID、Scopus、Medline、Web of Science等多个数据库以及精选的草药期刊中进行了全面的检索。从植物提取物中提取的绿色合成纳米颗粒的兴趣日益增长,这与本综述的重点有关,因此纳入草药期刊是合理的。对2015年至2023年间发表的文章进行了评估。为了确保稳健的选择过程,使用AXIS清单之外的特定标准评估研究的质量,包括研究设计、样本量和方法的严谨性。审稿人之间的任何差异都通过与第三审稿人的讨论或咨询来解决。该方法旨在最大限度地减少偏倚,并确保纳入对综述目标有意义的高质量研究。目的:本综述的主要目的是研究绿色合成的金属纳米颗粒,如银和锌纳米颗粒,在治疗龋齿方面的治疗意义,重点是它们的抗菌特性和改进当前治疗方式的潜力。材料与方法:本研究检索PubMed、谷歌Scholar、SID、Scopus、Medline、Web of Science、herbal medicine期刊,对2015 - 2023年的文章进行评价。结果:本研究回顾了22项临床试验,几乎所有的试验都证明了合成纳米颗粒的有效性。研究发现,对蛀牙细菌最有效的纳米颗粒包括银、锌、硒、镍和铜纳米颗粒。为了提供更清晰的信息,关键发现,如纳米颗粒大小、最小抑制浓度(MIC)值和特定的抗菌效果,在表格中列出。此外,对这些纳米颗粒进行了更深入的比较,重点研究了它们在不同条件下的相对有效性。例如,银纳米粒子由于其强大的抗菌性能,在各种试验中始终有效,而锌和铜纳米粒子在特定浓度下和对特定细菌菌株有效。此外,将绿色合成的纳米颗粒与传统方法生产的纳米颗粒进行比较,评估其有效性、可扩展性和安全性等因素,以全面了解其在龋齿治疗中的潜力。结论:根据文献综述,具有抗菌特性的纳米颗粒有可能作为传统化学治疗对抗蛀牙的替代或补充。未来的研究应侧重于探索特定类型的纳米颗粒,如绿色合成的金属纳米颗粒(如银和锌),它们已显示出良好的抗菌效果。此外,应更加重视优化合成方法,以提高临床应用的生物相容性和可扩展性。利用药用植物开发可负担得起的治疗方案进行绿色合成尤其有希望,应该进一步探索使基于纳米粒子的治疗方法在临床环境中更容易获得。
{"title":"The Effect of Green-Synthesized Nanoparticles on Dental Caries-Causing Bacteria: A Systematic Review.","authors":"Asma Sepahdar, Behnoush Selahbarzin, Atoosa Vaez Naini, Samira Jafari, Mehrnaz Moradi, Pegah Shakib","doi":"10.2174/0118722083343723241204053839","DOIUrl":"https://doi.org/10.2174/0118722083343723241204053839","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;In this study, a comprehensive search was conducted across multiple databases, including PubMed, Google Scholar, SID, Scopus, Medline, and Web of Science, as well as selected herbal medicine journals. The inclusion of herbal medicine journals was justified by the growing interest in green-synthesized nanoparticles derived from plant extracts, which are relevant to the focus of this review. Articles published between 2015 and 2023 were evaluated. To ensure a robust selection process, studies were assessed for quality using specific criteria beyond the AXIS checklist, including study design, sample size, and methodological rigor. Any discrepancies between reviewers were resolved through discussion or consultation with a third reviewer. This approach aimed to minimize bias and ensure the inclusion of high-quality studies that contribute meaningfully to the review's objectives.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;The primary objective of this review was to investigate the therapeutic implications of green-synthesized metallic nanoparticles, such as silver and zinc nanoparticles, in addressing dental caries, with a focus on their antibacterial properties and potential to improve current treatment modalities.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;In this study, PubMed, Google Scholar, SID, Scopus, Medline, Web of Science, and herbal medicine journals were searched, and articles from 2015 to 2023 were evaluated.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In this study, 22 clinical trials were reviewed, and the effectiveness of synthesized nanoparticles was demonstrated in nearly all of them. The nanoparticles found to be most effective against tooth decay-causing bacteria include silver, zinc, selenium, nickel, and copper nanoparticles. To provide greater clarity, key findings, such as nanoparticle size, minimum inhibitory concentration (MIC) values, and specific antibacterial effects, are presented in a table. Additionally, a more in-depth comparison was made between these nanoparticles, focusing on their relative effectiveness under different conditions. For example, silver nanoparticles were consistently effective across various trials due to their strong antimicrobial properties, while zinc and copper nanoparticles showed efficacy in specific concentrations and against particular bacterial strains. Furthermore, green-synthesized nanoparticles were compared with those produced using traditional methods, evaluating factors, such as effectiveness, scalability, and safety, to provide a comprehensive understanding of their potential in dental caries treatment.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;According to the literature review, nanoparticles with antibacterial properties have the potential to serve as an alternative or complement to conventional chemical treatments for combating tooth decay. Future research should focus on exploring specific types of nanoparticles, such as green-synthesized metallic nanoparticles (e.g., sil","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biosurfactants Used in the Bioremediation of Soils Contaminated With Hydrocarbons - Overview of the State of the Art and Future Perspectives. 生物表面活性剂用于烃类污染土壤的生物修复-现状综述和未来展望。
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-12-18 DOI: 10.2174/0118722083343490241130163344
Maria Auxiliadora de Oliveira, Vinícius Souza Tarabal, Felipe Ferreira Silva, Diego Fernandes Livio, Daniela Regiane da Silva, Adriano Guimarães Parreira, Daniel Bonoto Gonçalves, José Antônio da Silva, Pedro Pires Goulart Guimarães, Paulo Afonso Granjeiro

Background: The increasing industrialization and hydrocarbon use have led to concerning soil contamination. Oil spills and improper disposal of oily waste pose threats to ecosystems and human health. The recovery of these environments is essential, but separating oily components from soil remains challenging. Current bioremediation strategies using synthetic surfactants can cause secondary contamination. Microbial biosurfactants, which are biodegradable and low in toxicity, emerge as promising solutions, and this study reviews methods for utilizing these biosurfactants in the environmental bioremediation of hydrocarbons.

Objective: This study explores the efficient and eco-friendly use of biosurfactants for hydrocarbon- contaminated soil management, providing a market-oriented analysis of recent patents and trends, and highlighting the transition from academic research to industrial applications.

Method: The methodology involves an extensive literature review, careful selection of recent studies and patents on biosurfactants in hydrocarbon bioremediation, critical analysis of in-situ and ex-situ application methods, assessment of commercial viability, and synthesis of findings to contribute to sustainable solutions in contaminated environments.

Conclusion: The present study demonstrates the extensive applicability of biosurfactants across various industrial sectors. The increasing interest in incorporating biosurfactants into industrial processes is driven by the pressing need for sustainable solutions to address tangible market challenges. Notably, the cosmetics industry exhibited the highest number of patents related to the use of biosurfactants, underscoring its significant role in advancing the adoption of these environmentally friendly agents. This trend highlights the critical demand for sustainable alternatives in product formulations and underscores the pivotal role of biosurfactants in fostering eco-innovation within the industry.

背景:工业化和碳氢化合物利用的增加导致土壤污染日益严重。溢油和含油废物处置不当对生态系统和人类健康构成威胁。这些环境的恢复至关重要,但从土壤中分离含油成分仍然具有挑战性。目前使用合成表面活性剂的生物修复策略可能导致二次污染。微生物表面活性剂具有可生物降解和低毒性的特点,是一种很有前景的解决方案,本文综述了微生物表面活性剂在烃类环境生物修复中的应用方法。目的:本研究旨在探索生物表面活性剂在烃类污染土壤治理中的高效和生态利用,对其最新专利和趋势进行市场化分析,并强调从学术研究到工业应用的转变。方法:该方法包括广泛的文献综述,对碳氢化合物生物修复中生物表面活性剂的最新研究和专利进行仔细选择,对原位和非原位应用方法进行批判性分析,评估商业可行性,并将研究结果综合起来,为污染环境的可持续解决方案做出贡献。结论:本研究表明生物表面活性剂在各个工业部门具有广泛的适用性。由于迫切需要可持续的解决方案来应对切实的市场挑战,人们对将生物表面活性剂纳入工业过程的兴趣日益浓厚。值得注意的是,化妆品行业展示了与生物表面活性剂使用相关的专利数量最多,强调了其在推动采用这些环保剂方面的重要作用。这一趋势强调了对产品配方中可持续替代品的关键需求,并强调了生物表面活性剂在促进行业内生态创新方面的关键作用。
{"title":"Biosurfactants Used in the Bioremediation of Soils Contaminated With Hydrocarbons - Overview of the State of the Art and Future Perspectives.","authors":"Maria Auxiliadora de Oliveira, Vinícius Souza Tarabal, Felipe Ferreira Silva, Diego Fernandes Livio, Daniela Regiane da Silva, Adriano Guimarães Parreira, Daniel Bonoto Gonçalves, José Antônio da Silva, Pedro Pires Goulart Guimarães, Paulo Afonso Granjeiro","doi":"10.2174/0118722083343490241130163344","DOIUrl":"https://doi.org/10.2174/0118722083343490241130163344","url":null,"abstract":"<p><strong>Background: </strong>The increasing industrialization and hydrocarbon use have led to concerning soil contamination. Oil spills and improper disposal of oily waste pose threats to ecosystems and human health. The recovery of these environments is essential, but separating oily components from soil remains challenging. Current bioremediation strategies using synthetic surfactants can cause secondary contamination. Microbial biosurfactants, which are biodegradable and low in toxicity, emerge as promising solutions, and this study reviews methods for utilizing these biosurfactants in the environmental bioremediation of hydrocarbons.</p><p><strong>Objective: </strong>This study explores the efficient and eco-friendly use of biosurfactants for hydrocarbon- contaminated soil management, providing a market-oriented analysis of recent patents and trends, and highlighting the transition from academic research to industrial applications.</p><p><strong>Method: </strong>The methodology involves an extensive literature review, careful selection of recent studies and patents on biosurfactants in hydrocarbon bioremediation, critical analysis of in-situ and ex-situ application methods, assessment of commercial viability, and synthesis of findings to contribute to sustainable solutions in contaminated environments.</p><p><strong>Conclusion: </strong>The present study demonstrates the extensive applicability of biosurfactants across various industrial sectors. The increasing interest in incorporating biosurfactants into industrial processes is driven by the pressing need for sustainable solutions to address tangible market challenges. Notably, the cosmetics industry exhibited the highest number of patents related to the use of biosurfactants, underscoring its significant role in advancing the adoption of these environmentally friendly agents. This trend highlights the critical demand for sustainable alternatives in product formulations and underscores the pivotal role of biosurfactants in fostering eco-innovation within the industry.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antifungal Activity of Alcoholic Extract of Allium Jesdianum on Fungi-Contaminated Dairy Products. 大蒜醇提物对真菌污染乳制品的抑菌活性研究
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-12-18 DOI: 10.2174/0118722083332626241129061731
Fatemeh Fallahi, Shirin Naghdifar, Zahra Sadri Irani, Seyed Mohammad Mahdi Kazemi, Pegah Shakib, Mahboobeh Madani

Background: One of the main sources of contaminated dairy products is moldy fungi, specifically species of Aspergillus and Penicillium. This study aimed to evaluate the effect of the alcoholic extract of Allium jesdianum plant on the growth of molds contaminating dairy products in Isfahan.

Materials and methods: In this research, 200 samples of dairy products were gathered from different areas of Isfahan city, including 70 samples of cheese, 60 samples of buttermilk, 40 samples of yogurt, 20 samples of curd, and 10 samples of cream. The antifungal activity of Allium jesdianum plant was investigated by the diffusion method in disc, well, and agar dilution in various concentrations. Minimum inhibitory concentration (MIC) and minimum fatal concentration (MFC) were also determined. Aspergillus, Penicillium, Cladosporium, and Acremonium fungi were the most commonly found fungal contaminants of this investigation. Antifungal activity was not observed by disc diffusion and well diffusion methods.

Results: In the agar dilution method, ethanolic and methanolic extracts of stem and leaves in concentrations of 80, 60, 40, and 30 mg/ml, and ethanolic and methanolic extracts of plant bulbs in concentrations of 60 and 30 mg/ml revealed antifungal activity against Aspergillus niger, Penicillium notatum, and Penicillium chrysogenum. The MIC of stem and leaf ethanol extracts and onion ethanol for Aspergillus niger was 18.7, and for Penicillium notatum and Penicillium chrysogenum, it was 37.5, 37.5, 37.5, and 37.5 mg/ml. Fungal contamination of dairy products is a serious threat to the public health of society. Therefore, identifying medicinal plants with antifungal activity can be an effective step in preventing fungal contamination and increasing the shelf life of these products.

Conclusion: The results of this research have shown that the Allium jesdianum plant can inhibit the growth of Aspergillus niger, Penicillium notatum, and Penicillium chrysogenum.

背景:乳制品污染的主要来源之一是发霉真菌,特别是曲霉和青霉。本研究旨在评价葱属植物醇提物对伊斯法罕地区乳制品霉菌污染的影响。材料与方法:本研究从伊斯法罕市不同地区采集了200份乳制品样品,其中奶酪70份,酪乳60份,酸奶40份,凝乳20份,奶油10份。采用圆盘扩散法、孔扩散法和不同浓度琼脂稀释法研究了大蒜的抑菌活性。测定了最小抑制浓度(MIC)和最小致死浓度(MFC)。曲霉、青霉菌、枝孢菌和顶孢菌是本次调查中最常见的真菌污染物。圆盘扩散法和孔扩散法均未观察到抑菌活性。结果:琼脂稀释法中,茎叶乙醇和甲醇提取物浓度分别为80、60、40、30 mg/ml,球茎乙醇和甲醇提取物浓度分别为60、30 mg/ml,对黑曲霉、青霉、黄曲青霉均有较强的抗真菌活性。茎叶乙醇提取物和洋葱乙醇对黑曲霉的MIC值分别为18.7,对青霉菌和青霉菌的MIC值分别为37.5、37.5、37.5和37.5 mg/ml。乳制品真菌污染严重威胁着社会公众健康。因此,鉴定具有抗真菌活性的药用植物是防止真菌污染和延长产品保质期的有效步骤。结论:本研究结果表明,葱属植物对黑曲霉、青霉菌、青霉菌的生长具有抑制作用。
{"title":"Antifungal Activity of Alcoholic Extract of Allium Jesdianum on Fungi-Contaminated Dairy Products.","authors":"Fatemeh Fallahi, Shirin Naghdifar, Zahra Sadri Irani, Seyed Mohammad Mahdi Kazemi, Pegah Shakib, Mahboobeh Madani","doi":"10.2174/0118722083332626241129061731","DOIUrl":"https://doi.org/10.2174/0118722083332626241129061731","url":null,"abstract":"<p><strong>Background: </strong>One of the main sources of contaminated dairy products is moldy fungi, specifically species of Aspergillus and Penicillium. This study aimed to evaluate the effect of the alcoholic extract of Allium jesdianum plant on the growth of molds contaminating dairy products in Isfahan.</p><p><strong>Materials and methods: </strong>In this research, 200 samples of dairy products were gathered from different areas of Isfahan city, including 70 samples of cheese, 60 samples of buttermilk, 40 samples of yogurt, 20 samples of curd, and 10 samples of cream. The antifungal activity of Allium jesdianum plant was investigated by the diffusion method in disc, well, and agar dilution in various concentrations. Minimum inhibitory concentration (MIC) and minimum fatal concentration (MFC) were also determined. Aspergillus, Penicillium, Cladosporium, and Acremonium fungi were the most commonly found fungal contaminants of this investigation. Antifungal activity was not observed by disc diffusion and well diffusion methods.</p><p><strong>Results: </strong>In the agar dilution method, ethanolic and methanolic extracts of stem and leaves in concentrations of 80, 60, 40, and 30 mg/ml, and ethanolic and methanolic extracts of plant bulbs in concentrations of 60 and 30 mg/ml revealed antifungal activity against Aspergillus niger, Penicillium notatum, and Penicillium chrysogenum. The MIC of stem and leaf ethanol extracts and onion ethanol for Aspergillus niger was 18.7, and for Penicillium notatum and Penicillium chrysogenum, it was 37.5, 37.5, 37.5, and 37.5 mg/ml. Fungal contamination of dairy products is a serious threat to the public health of society. Therefore, identifying medicinal plants with antifungal activity can be an effective step in preventing fungal contamination and increasing the shelf life of these products.</p><p><strong>Conclusion: </strong>The results of this research have shown that the Allium jesdianum plant can inhibit the growth of Aspergillus niger, Penicillium notatum, and Penicillium chrysogenum.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial Effect of Formononetin Against the Periodental Pathogens Enterococcus faecalis and Candida albicans. 刺芒柄花素对牙周病原菌粪肠球菌和白色念珠菌的抗菌作用。
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-12-18 DOI: 10.2174/0118722083342082241129060918
Negar Delfan, Maryam Dalaei Moghadam, Pegah Shakib, Asma Sepahdar, Zahra Naghibeiranvand

Background: Among the bioactive isoflavones identified from different plants is formononetin. Formononetin's antioxidant, anti-inflammatory, and anti-cancer qualities have all received a lot of attention lately. The goal of the current investigation was to examine formononetin's antifungal and antibacterial activity against Candida albicans and Enterococcus faecalis in vitro, respectively.

Methods: The present study determined the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Minimum Fungicidal Concentration (MFC) of formononetin, chlorhexidine, formononetin + chlorhexidine, Formononetin+ nystatin, and nystatin against E. faecalis and C. albicans isolates. Next, SPSS version 25 was used to analyze the data. A significance cutoff of P<0.05 was taken into account.

Results: Chlorhexidine + formononetin and formononetin alone had the greatest and lowest minimum inhibitory concentrations (MIC) against E. faecalis, at 6.6 and 18.3 μg/ml, respectively. With MIC values of 0.66 and 8.3 μg/ml against a C. albicans isolate sensitive to nystatin, the combination of formononetin + nystatin and formononetin alone was shown to have the greatest and lowest anti-candida effects.

Conclusion: The findings demonstrated a considerably greater antibacterial effect (P<0.05) for the combinations of formononetin + nystatin and chlorhexidine as compared to nystatin and chlorhexidine alone.

背景:从不同植物中鉴定出的具有生物活性的异黄酮中有芒柄花素。刺芒柄花素的抗氧化、抗炎和抗癌特性最近都受到了很多关注。本研究的目的是研究刺芒柄花素对白色念珠菌和粪肠球菌的体外抗真菌和抗菌活性。方法:测定刺芒柄花素、氯己定、刺芒柄花素+氯己定、刺芒柄花素+制霉菌素和制霉菌素对分离株粪大肠杆菌和白色念珠菌的最小抑菌浓度(MIC)、最小杀菌浓度(MBC)和最小杀真菌浓度(MFC)。其次,使用SPSS version 25对数据进行分析。结果:氯己定+刺芒柄花素和单用刺芒柄花素对粪肠杆菌的最低抑制浓度(MIC)分别为6.6和18.3 μg/ml。对制霉菌素敏感的白色念珠菌的MIC值分别为0.66和8.3 μg/ml,刺芒柄花素+制霉菌素和单用刺芒柄花素的抗假丝酵母菌效果最好和最低。结论:本研究结果显示了较好的抗菌效果(P
{"title":"Antimicrobial Effect of Formononetin Against the Periodental Pathogens Enterococcus faecalis and Candida albicans.","authors":"Negar Delfan, Maryam Dalaei Moghadam, Pegah Shakib, Asma Sepahdar, Zahra Naghibeiranvand","doi":"10.2174/0118722083342082241129060918","DOIUrl":"https://doi.org/10.2174/0118722083342082241129060918","url":null,"abstract":"<p><strong>Background: </strong>Among the bioactive isoflavones identified from different plants is formononetin. Formononetin's antioxidant, anti-inflammatory, and anti-cancer qualities have all received a lot of attention lately. The goal of the current investigation was to examine formononetin's antifungal and antibacterial activity against Candida albicans and Enterococcus faecalis in vitro, respectively.</p><p><strong>Methods: </strong>The present study determined the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Minimum Fungicidal Concentration (MFC) of formononetin, chlorhexidine, formononetin + chlorhexidine, Formononetin+ nystatin, and nystatin against E. faecalis and C. albicans isolates. Next, SPSS version 25 was used to analyze the data. A significance cutoff of P<0.05 was taken into account.</p><p><strong>Results: </strong>Chlorhexidine + formononetin and formononetin alone had the greatest and lowest minimum inhibitory concentrations (MIC) against E. faecalis, at 6.6 and 18.3 μg/ml, respectively. With MIC values of 0.66 and 8.3 μg/ml against a C. albicans isolate sensitive to nystatin, the combination of formononetin + nystatin and formononetin alone was shown to have the greatest and lowest anti-candida effects.</p><p><strong>Conclusion: </strong>The findings demonstrated a considerably greater antibacterial effect (P<0.05) for the combinations of formononetin + nystatin and chlorhexidine as compared to nystatin and chlorhexidine alone.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the Characteristics of Circulating Small Extracellular Vesicles Isolated by Ultracentrifugation and a Commercial Kit. 比较超速离心法和商用试剂盒分离的循环小细胞外囊泡的特征
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-10-25 DOI: 10.2174/0118722083325164241015103217
Reza Afrisham, Vida Farrokhi, Roya Moradi, Shaban Alizadeh

Introduction: The market offers a wide range of extracellular vesicles (EVs) isolation products, but their lack of standardization is a concern. Therefore, it is important to carefully assess the quality of the EVs obtained using these products. In this study, we compared the EXOCIB kit with the ultracentrifuge method, which is considered the gold standard for small EV isolation.

Methods: After overnight fasting, small plasma EVs were extracted from four individuals using both the ultracentrifuge and the EXOCIB kit methods. The pooled EVs were then compared for the presence of the cluster of differentiation 63 (CD63) protein using the western blot analysis, and their size and zeta potential were performed by Dynamic Light Scattering (DLS). In addition, the size and morphology of small EVs were determined by using the Transmission Electron Microscopy (TEM) technique.

Results: An average hydrodynamic size of 135.7 nm and a zeta potential of -6.33 Mv at 25°C was found for small EVs isolated by the ultracentrifuge, whereas the kit method resulted in small EVs with a hydrodynamic size of 102.8 nm and a zeta potential of -0.907. Notably, the size of the particles in the kit samples was smaller compared to those obtained through the ultracentrifuge (P < 0.001). The western blot method confirmed the expression of CD63 in both methods, so the ultracentrifuge yielded small EVs with a higher level of purity compared to the kit-based approach (P = 0.036).

Conclusion: The DLS findings revealed the existence of vesicles within the appropriate size range for small EVs like exosomes in both isolation techniques. The results of the western blot analysis, in conjunction with DLS, displayed that the ultracentrifuge method extracted small EVs with a greater degree of purity than the kit-based approach.

简介:市场上有各种各样的细胞外囊泡 (EVs) 分离产品,但它们缺乏标准化令人担忧。因此,仔细评估使用这些产品获得的 EVs 的质量非常重要。在本研究中,我们将 EXOCIB 试剂盒与超速离心法进行了比较,后者被认为是分离小体积 EV 的金标准:过夜禁食后,使用超速离心机和 EXOCIB 试剂盒两种方法从四个人的血浆中提取小的 EVs。方法:在一夜禁食后,使用超速离心机和 EXOCIB 试剂盒两种方法从四个人的血浆中提取小的 EVs,然后使用 Western 印迹分析比较汇集的 EVs 是否含有分化簇 63(CD63)蛋白,并使用动态光散射(DLS)分析其大小和 zeta 电位。此外,还利用透射电子显微镜(TEM)技术测定了小型 EVs 的大小和形态:结果:通过超速离心机分离出的小型 EVs 的平均流体力学尺寸为 135.7 nm,25°C 时的 zeta 电位为 -6.33 Mv,而试剂盒法分离出的小型 EVs 的流体力学尺寸为 102.8 nm,zeta 电位为 -0.907。值得注意的是,与超速离心机相比,试剂盒样品中的颗粒尺寸更小(P < 0.001)。Western 印迹法确认了两种方法中 CD63 的表达,因此与试剂盒法相比,超速离心法得到的小 EV 的纯度更高(P = 0.036):结论:DLS 研究结果表明,两种分离技术都能分离出适当大小范围内的囊泡,如外泌体等小型 EV。结合 DLS 进行的 Western 印迹分析结果表明,超速离心法提取的小型 EV 的纯度高于试剂盒法。
{"title":"Comparison of the Characteristics of Circulating Small Extracellular Vesicles Isolated by Ultracentrifugation and a Commercial Kit.","authors":"Reza Afrisham, Vida Farrokhi, Roya Moradi, Shaban Alizadeh","doi":"10.2174/0118722083325164241015103217","DOIUrl":"https://doi.org/10.2174/0118722083325164241015103217","url":null,"abstract":"<p><strong>Introduction: </strong>The market offers a wide range of extracellular vesicles (EVs) isolation products, but their lack of standardization is a concern. Therefore, it is important to carefully assess the quality of the EVs obtained using these products. In this study, we compared the EXOCIB kit with the ultracentrifuge method, which is considered the gold standard for small EV isolation.</p><p><strong>Methods: </strong>After overnight fasting, small plasma EVs were extracted from four individuals using both the ultracentrifuge and the EXOCIB kit methods. The pooled EVs were then compared for the presence of the cluster of differentiation 63 (CD63) protein using the western blot analysis, and their size and zeta potential were performed by Dynamic Light Scattering (DLS). In addition, the size and morphology of small EVs were determined by using the Transmission Electron Microscopy (TEM) technique.</p><p><strong>Results: </strong>An average hydrodynamic size of 135.7 nm and a zeta potential of -6.33 Mv at 25°C was found for small EVs isolated by the ultracentrifuge, whereas the kit method resulted in small EVs with a hydrodynamic size of 102.8 nm and a zeta potential of -0.907. Notably, the size of the particles in the kit samples was smaller compared to those obtained through the ultracentrifuge (P < 0.001). The western blot method confirmed the expression of CD63 in both methods, so the ultracentrifuge yielded small EVs with a higher level of purity compared to the kit-based approach (P = 0.036).</p><p><strong>Conclusion: </strong>The DLS findings revealed the existence of vesicles within the appropriate size range for small EVs like exosomes in both isolation techniques. The results of the western blot analysis, in conjunction with DLS, displayed that the ultracentrifuge method extracted small EVs with a greater degree of purity than the kit-based approach.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curcumin-assisted Preparation of α-Fe2O3@TiO2 Nanocomposites for Antibacterial and Photocatalytic Activity. 姜黄素辅助制备具有抗菌和光催化活性的 α-Fe2O3@TiO2 纳米复合材料
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-10-18 DOI: 10.2174/0118722083332040241011050802
Anuskha Kala, Krati Saini, Sanjeev Kimothi, Rashmi Verma, Kamal K Kar, Pankaj Chamoli

Background: Harmful microorganisms like pathogens significantly impact human health. Meanwhile, industrial growth causes pollution and water contamination by releasing untreated hazardous waste. Effective treatment of these microorganisms and contaminants is essential, and nanocomposites may be a promising solution. The present attempt demonstrates the green synthesis of α-Fe2O3@TiO2 nanocomposites (FTNCs) for the effective treatment of pathogens and organic contaminants.

Methods: The α-Fe2O3@TiO2 nanocomposites (FTNCs) has been synthesized through a green approach utilizing curcumin extract. Curcumin (Turmeric) extract (TEx) was prepared by washing, drying, and crushing 5 g of turmeric, then boiling it in 100 mL distilled water at 70°C for 1 hour. Metal salts (Fe3+/Ti4+, 2:1) were added to 100 mL of TEx under continuous stirring at 70°C for 24 h. The solution was rinsed and dried at 80°C overnight and heated at 300°C for 3 h to remove impurities.

Results: Synthesized FTNCs have been tested for the potent antibacterial activity against both Gram-positive (Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis) and Gram-negative bacteria (Escherichia coli, Salmonella Abony, Pseudomonas sp.). Observations discovered noteworthy inhibition of both Gram-positive and Gramnegative bacteria by FTNCs. Furthermore, the FTNCs system shows the energy band gap of ~2.6 eV which may suppress electron recombination, thereby enhancing photocatalysis and examined against Evans blue (EB) and Congo red (CR) dyes under UV and visible light (125 W) irradiation. The remarkable photocatalytic degradation efficiency (DE) for CR reached ~67.4% in 60 min.

Conclusion: A simple green approach has been demonstrated for the synthesis of the FTNCs using curcumin-mediated reduction. As prepared FTNCs have been evaluated for potent antibacterial activity against both Gram-positive (Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis) and Gram-negative bacteria (Escherichia coli, Salmonella Abony, Pseudomonas sp.).

Observations: The results show that the highest ZID values have been obtained for 5 mg/mL concertation of FTNCs of ~14, 22,18, 21, and 20 and 29 mm for E. coli, S. abony, S. aureus, B. subtilis, E. faecalis, and Pseudomonas sp., respectively. Additionally, FTNCs demonstrate remarkable photocatalytic degradation efficiency against EB and CR dyes under UV (125 W) irradiation, achieving 56, 67% degradation within 60 minutes for EB and CR. The findings suggest that the FTNCs hold promise for long-term antimicrobial efficacy against various bacteria and offer the potential for addressing water and wastewater contaminants through photocatalysis.

背景:病原体等有害微生物严重影响人类健康。与此同时,工业增长释放出未经处理的有害废物,造成污染和水污染。有效处理这些微生物和污染物至关重要,而纳米复合材料可能是一种很有前景的解决方案。本研究尝试绿色合成 α-Fe2O3@TiO2 纳米复合材料(FTNCs),以有效处理病原体和有机污染物:方法:α-Fe2O3@TiO2纳米复合材料(FTNCs)是利用姜黄素提取物通过绿色方法合成的。姜黄素(姜黄)提取物(TEx)的制备方法是:将 5 克姜黄洗净、烘干并粉碎,然后在 100 毫升蒸馏水中以 70°C 的温度煮沸 1 小时。将金属盐(Fe3+/Ti4+,2:1)加入 100 mL TEx 溶液中,在 70°C 下持续搅拌 24 小时:合成的 FTNCs 对革兰氏阳性菌(金黄色葡萄球菌、枯草芽孢杆菌、粪肠球菌)和革兰氏阴性菌(大肠杆菌、沙门氏菌、假单胞菌)都具有强效抗菌活性。观察发现,FTNCs 对革兰氏阳性和革兰氏阴性细菌都有显著抑制作用。此外,FTNCs 系统显示出 ~2.6 eV 的能带间隙,可抑制电子重组,从而增强光催化作用,并在紫外线和可见光(125 W)照射下对伊文思蓝(EB)和刚果红(CR)染料进行了检测。在 60 分钟内,CR 的光催化降解效率(DE)达到了约 67.4%:结论:利用姜黄素介导的还原法合成 FTNCs 是一种简单的绿色方法。所制备的 FTNCs 对革兰氏阳性菌(金黄色葡萄球菌、枯草芽孢杆菌、粪肠球菌)和革兰氏阴性菌(大肠埃希菌、沙门氏菌、假单胞菌)都具有很强的抗菌活性:结果显示,5 毫克/毫升的 FTNCs 协同作用对大肠杆菌、沙门氏菌、金黄色葡萄球菌、枯草杆菌、粪肠球菌和假单胞菌的 ZID 值最高,分别为 14、22、18、21、20 和 29 毫米。此外,在紫外线(125 瓦)照射下,FTNCs 对 EB 和 CR 染料的光催化降解效率非常高,在 60 分钟内对 EB 和 CR 的降解率分别达到 56%和 67%。研究结果表明,FTNCs 有望对各种细菌产生长期抗菌效果,并为通过光催化处理水和废水污染物提供了潜力。
{"title":"Curcumin-assisted Preparation of α-Fe2O3@TiO2 Nanocomposites for Antibacterial and Photocatalytic Activity.","authors":"Anuskha Kala, Krati Saini, Sanjeev Kimothi, Rashmi Verma, Kamal K Kar, Pankaj Chamoli","doi":"10.2174/0118722083332040241011050802","DOIUrl":"https://doi.org/10.2174/0118722083332040241011050802","url":null,"abstract":"<p><strong>Background: </strong>Harmful microorganisms like pathogens significantly impact human health. Meanwhile, industrial growth causes pollution and water contamination by releasing untreated hazardous waste. Effective treatment of these microorganisms and contaminants is essential, and nanocomposites may be a promising solution. The present attempt demonstrates the green synthesis of α-Fe2O3@TiO2 nanocomposites (FTNCs) for the effective treatment of pathogens and organic contaminants.</p><p><strong>Methods: </strong>The α-Fe2O3@TiO2 nanocomposites (FTNCs) has been synthesized through a green approach utilizing curcumin extract. Curcumin (Turmeric) extract (TEx) was prepared by washing, drying, and crushing 5 g of turmeric, then boiling it in 100 mL distilled water at 70°C for 1 hour. Metal salts (Fe3+/Ti4+, 2:1) were added to 100 mL of TEx under continuous stirring at 70°C for 24 h. The solution was rinsed and dried at 80°C overnight and heated at 300°C for 3 h to remove impurities.</p><p><strong>Results: </strong>Synthesized FTNCs have been tested for the potent antibacterial activity against both Gram-positive (Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis) and Gram-negative bacteria (Escherichia coli, Salmonella Abony, Pseudomonas sp.). Observations discovered noteworthy inhibition of both Gram-positive and Gramnegative bacteria by FTNCs. Furthermore, the FTNCs system shows the energy band gap of ~2.6 eV which may suppress electron recombination, thereby enhancing photocatalysis and examined against Evans blue (EB) and Congo red (CR) dyes under UV and visible light (125 W) irradiation. The remarkable photocatalytic degradation efficiency (DE) for CR reached ~67.4% in 60 min.</p><p><strong>Conclusion: </strong>A simple green approach has been demonstrated for the synthesis of the FTNCs using curcumin-mediated reduction. As prepared FTNCs have been evaluated for potent antibacterial activity against both Gram-positive (Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis) and Gram-negative bacteria (Escherichia coli, Salmonella Abony, Pseudomonas sp.).</p><p><strong>Observations: </strong>The results show that the highest ZID values have been obtained for 5 mg/mL concertation of FTNCs of ~14, 22,18, 21, and 20 and 29 mm for E. coli, S. abony, S. aureus, B. subtilis, E. faecalis, and Pseudomonas sp., respectively. Additionally, FTNCs demonstrate remarkable photocatalytic degradation efficiency against EB and CR dyes under UV (125 W) irradiation, achieving 56, 67% degradation within 60 minutes for EB and CR. The findings suggest that the FTNCs hold promise for long-term antimicrobial efficacy against various bacteria and offer the potential for addressing water and wastewater contaminants through photocatalysis.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Recent patents on biotechnology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1