Antimicrobial resistance is a critical and global public health challenge, contributing to treatment failure, prolonged hospitalizations, and increased healthcare costs. Consequently, the discovery and development of new antibiotics is urgently needed. Recent studies have highlighted marine sponges as prolific sources of bioactive compounds and important reservoirs of actinomycetes, a rich source of antibiotics. In this study, 50 sponge samples were collected from Mun Nork and Mun Nai Islands in Rayong Province, Thailand, and evaluated for their antimicrobial activity and potential to harbor sponge-associated actinomycetes. Of the samples tested, 30 sponge extracts exhibited antimicrobial activity, predominantly against Gram-positive bacteria. Notably, Neopetrosia sp. (blue) demonstrated the broadest spectrum of activity, inhibiting Gram-positive and Gram-negative bacteria as well as yeast. A total of 41 actinomycete isolates were obtained from 15 sponge samples and subsequently classified into 12 genera: Micromonospora (3 isolates), Nocardia (13), Rothia (1), Isoptericola (1), Salinispora (12), Actinomycetospora (1), Pseudonocardia (1), Streptomyces (5), Mycobacterium (1), Prauserella (1), and Rhodococcus (2). Among these, three strains — Streptomyces (Strept.) althioticus RS027–1, Strept. nanshensis TR01–1, and Salinispora arenicola RS029–1 — exhibited antimicrobial activity against Kocuria rhizophila and Streptococcus pyogenes. Additionally, Salinispora arenicola RS029–1 showed broader activity, inhibiting Bacillus subtilis and Staphylococcus aureus. Based on 16S rRNA gene sequence analysis, three isolates were identified as potential novel species. The findings demonstrate that marine sponges are valuable sources of antimicrobial compounds and serve as important habitats for bioactive actinomycetes. Furthermore, marine sponges hold considerable promise for the discovery of novel actinomycete species with potential applications in future drug discovery and development.
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