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Physicochemical analysis and microbial quality of raw cow's milk along with production system in Angolelanatera district, Amhara region, Ethiopia 埃塞俄比亚阿姆哈拉地区Angolelanatera地区生牛奶的理化分析和微生物质量及其生产系统
Pub Date : 2016-10-20 DOI: 10.14196/sjas.v5i10.2319
G. Kassa, Tewodros Eshete, L. Gazu
This study was conducted to determine physicochemical analysis and microbial quality of raw cow’s milk along with the production system in the study area. Data was collected by conducting experimental activities and survey. Statistical package for social science (SPSS) were used to analyze data. The main income sources of the respondents were mixed agriculture (92.5%) and 44.5% respondents had basic education. 78.3%  respondents believed the milk quality of local cows were better than cross cows. Natural grazing, crop residues and conserved hay (54.6, 41.8 and 3.6) % were the main feed resources in the study area respectively. The only improved forage species were oat and vetch but most respondents feed these forage species after the seed was collected. The maximum mean of fat and protein content of cow’s raw milk in the study areas were 5.30±0.28 and 3.29±0.05 in Chekiy local cows and Chefanen cross cows respectively. The highest total bacterial and coli form count was recorded in Tsigereeda and Chacha kebeles (3.75x10 8 and 2.16x 10 5 ) respectively. The average number of yeast and mold was 1.56x10 5 , 1.97x10 5 , 2.14x10 5 and 1.73x10 5 in Tsigereda, Chekiey, Chefanen and Chacha respectively. The highest bacterial and coli form count was recorded due to poor hygienic practice. Interventions about hygienic practice will solve the listed problems.
本研究对研究地区生牛奶的理化分析和微生物质量进行了测定,并对生产系统进行了分析。通过实验活动和调查收集数据。采用SPSS软件对数据进行分析。受访者的主要收入来源为混合农业(92.5%),44.5%的受访者受过基础教育。78.3%的受访者认为本地奶牛的牛奶质量好于杂交奶牛。自然放牧、作物残茬和保存干草是研究区主要的饲料资源(分别占54.6%、41.8%和3.6%)。改良的饲料品种只有燕麦和野豌豆,但大多数回答者在采集种子后再饲喂这些饲料。研究区契诃夫本地奶牛和契诃夫杂交奶牛原料奶脂肪和蛋白质含量的最高平均值分别为5.30±0.28和3.29±0.05。细菌总数和大肠杆菌总数最高的分别是齐格氏菌(3.75 × 10 8)和恰氏菌(2.16 × 10 5)。Tsigereda、Chekiey、Chefanen和Chacha的酵母和霉菌平均数量分别为1.56 × 10 5、1.97 × 10 5、2.14 × 10 5和1.73 × 10 5。由于卫生习惯不佳,细菌和大肠杆菌数量最高。卫生行为干预将解决上述问题。
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引用次数: 0
Feeding detoxified Ethiopian mustard (Brassica carinata) seed cake to sheep: Effect on intake, digestibility, live weight gain and carcass parameters 饲喂解毒芥菜籽饼对绵羊采食量、消化率、活增重和胴体参数的影响
Pub Date : 2016-09-20 DOI: 10.14196/SJAS.V5I9.2311
W. Bekele, G. Assefa, M. Urgie
In Ethiopia, cultivation and usage of B. carinata is an old practice. The seeds are primarily cultivated for the extraction of oil, the meal that is remained after oil extraction is protein rich (30 – 45%) and may be used either as high protein feed supplement provided that glucosinolate level is reduced or as organic fertilizer. Oil extraction through the use of solvents has proven to be beneficial in improving the feed quality of the oil seed cake produced as by product. This is as a result of removing the anti-nutritional qualities like glucosinolates by the solvents. These technologies are under evaluation at pilot level in Ethiopia. Thus, a study was conducted to investigate the effect of including both untreated and detoxified Ethiopian mustard seed (Brassica carinata) cake as protein source in the concentrate supplement on feed intake, digestibility, and live weight gain using thirty yearling Afar male sheep with an average initial body weight of 17.1 + 0.6 kg. A completely randomized block design with six replicates per treatment was used. The treatments were: 0, 33, 67 and 100% replacement of noug cake by detoxified mustard cake and 67% replacement by undetoxified cake. All animals were fed and managed in individual pens. Feed intake, apparent digestibility, growth rate and carcass parameters did not differ among treatments. The results of the study showed that detoxified Ethiopian mustard seed cake can completely replace noug seed cake when offered in a mixture with wheat bran for growing  male sheep fed a basal diet of grass hay.
在埃塞俄比亚,种植和使用B. carinata是一种古老的做法。种植种子主要是为了榨油,榨油后剩下的粕富含蛋白质(30 - 45%),在降低硫代葡萄糖苷水平的情况下,可作为高蛋白饲料补充或用作有机肥。经证明,采用溶剂提取油脂有利于提高副产品油籽饼的饲料质量。这是由于溶剂去除了硫代葡萄糖苷等抗营养物质。这些技术正在埃塞俄比亚试点阶段进行评价。因此,本研究以30只平均初始体重为17.1 + 0.6 kg的阿法尔公羊为试验对象,研究在精料补充中添加未经处理和解毒的埃塞俄比亚芥菜籽饼作为蛋白质源对采食量、消化率和活增重的影响。采用完全随机区组设计,每个处理6个重复。处理分别为:0、33、67和100%用脱毒芥末饼替代诺格饼,67%用未脱毒芥末饼替代。所有动物在单独的围栏内饲养和管理。采食量、表观消化率、生长率和胴体参数各处理间无显著差异。研究结果表明,脱毒的埃塞俄比亚芥菜籽饼与麦麸混合后,可以完全取代以干草为基础日粮的生长雄性绵羊的种子饼。
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引用次数: 5
Prevalence and associated risk factors for soil transmitted Helminth infections among Urban Dwellers, Amanuel Town, Northwest Ethiopia 埃塞俄比亚西北部Amanuel镇城市居民土壤传播性蠕虫感染的流行情况及相关危险因素
Pub Date : 2016-09-18 DOI: 10.14196/sjbs.v5i9.2303
Yirga Enawgaw, A. Sisay, E. Nibret
Infection by soil transmitted helminthes has been increasingly recognized as an important public health problem in poor and developing countries. To initiate prevention and control measures for these neglected diseases, adequate information is required among high risk group. The objective of this study was to determine the prevalence and associated risk factors for soil transmitted helminth infections among urban dwellers in Amanuel, east Gojjam, Ethiopia. A cross-sectional study, involving 403 individuals, was carried out in Amanuel between November 2010 and January 2011. A structured questionnaire and observation for demographic and associated risk factors for soil transmitted helminthes (STHs) infections were used for data collection. Stool samples were collected in plastic containers from each participant and transported to Amanuel Health center for parasitological examination. Both direct wet smear and former-ether sedimentation concentration techniques were used to determine the presence of helminthes eggs. The overall prevalence of STH infections was 46.2% (186/403). Hookworm infection was the predominant soil transmitted helminth infections, which was detected in 77(19.1%) followed by A. lumbricoides which was infected 52(13%) of the study subjects. T. trichuria was the least prevalent geo-helminth infection, detected in only 23 (5.7%) of the study subjects. Majorities, 152(37.7%), had single infection. Double infection occurred in 25(6.2%) of the study subjects and only 9(2.2%) had multiple infection. The prevalence of soil transmitted helminthes was generally high in school age children and low in infants. The common predictors of STH infections in the study, among others, were poor personal hygienic conditions, less frequent wearing shoes habit, soil contacting occupation risk, and lack of hand washing habit after using toilet. On the basis of this result, it can be concluded that STH infection is an important problem among the community of Amanuel town. Therefore, it is recommended that education on personal and environmental hygiene should be taken into account to reduce the prevalence of STH infections. Moreover, school age children and other risk groups should receive regular treatment to control the transmissions of STH infections.
土壤传播的蠕虫感染已日益被认为是贫穷和发展中国家的一个重要公共卫生问题。为了对这些被忽视的疾病采取预防和控制措施,需要向高危人群提供充分的信息。本研究的目的是确定埃塞俄比亚Gojjam东部Amanuel城市居民中土壤传播蠕虫感染的流行情况和相关危险因素。2010年11月至2011年1月,在阿曼纽尔进行了一项涉及403人的横断面研究。对土壤传播蠕虫(STHs)感染的人口统计学和相关危险因素进行了结构化问卷调查和观察,以收集数据。将每个参与者的粪便样本收集在塑料容器中,并运送到Amanuel卫生中心进行寄生虫学检查。采用直接湿涂片法和前醚沉降浓缩法测定虫卵的存在。总感染率为46.2%(186/403)。土壤传播性寄生虫感染以钩虫感染为主,77例(19.1%),其次是蚓类,52例(13%)。滴虫是最不常见的地蠕虫感染,仅在23例(5.7%)研究对象中检测到。单次感染152例(37.7%),占多数。25例(6.2%)发生双重感染,9例(2.2%)发生多重感染。土壤传播蠕虫的流行率在学龄儿童中普遍较高,在婴儿中较低。在研究中,STH感染的常见预测因素包括个人卫生条件差、不经常穿鞋的习惯、接触土壤的职业风险以及如厕后缺乏洗手习惯。在此基础上,可以得出结论,STH感染是Amanuel镇社区的一个重要问题。因此,我们建议加强个人和环境卫生教育,以减少STH感染的流行。此外,学龄儿童和其他危险人群应定期接受治疗,以控制STH感染的传播。
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引用次数: 1
Influence of age on sperm production's indicators of bulls 年龄对公牛精子生产指标的影响
Pub Date : 2016-08-18 DOI: 10.14196/SJAS.V5I8.2271
M. Aidos, S. Aljan, Alshinbaev Orynbasar
The results obtained in the study age dynamics of indicators of sperm production of breeding bulls of black-motley and hill breeds indicate on the Republican Center of livestock breeding JSC "ASIL TYLІK" Akmola Province, Kazakhstan indicate that the quantity of the received sperm production changes by years of their use. Special differences of age dynamics between breeds it isn't observed.
在哈萨克斯坦Akmola省共和畜牧育种中心JSC“ASIL TYLІK”上对黑杂色和山地种种牛精子产量指标的年龄动态研究结果表明,接受的精子产量随使用年限的变化而变化。没有观察到不同品种之间年龄动态的特殊差异。
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引用次数: 2
Research properties of the environment on basis of vegetable components from extract soy at the cryopreservation of sperm of bulls 以大豆提取物植物成分为基础研究公牛精子低温保存的环境特性
Pub Date : 2016-08-18 DOI: 10.14196/sjas.v5i8.2279
M. Aidos, S. Aljan, Alshinbaev Orynbasar
By cryobiological, immunological and biophysical methods it is established that in the mechanism of temperature shock of cell the leading role is assigned to osmosis, and instant movement of water through it at the expense of an equilibration of osmotic pressure that is the reason of destruction of a cell membrane deprives a cytoplasmic membrane of properties of semipermeability. It was studied the fortification mechanism sperm when cooling and it is established that the fortificant accumulates on surfaces of a cell and creates a hydrophobic phase that slows down the osmotic action connected with an balancing  of concentration gradients in ''cell-wednesday'' system. It is established, also, that the protective layer is created on a cytoplasmatic membrane on an extent of 2-5 minutes from the moment of its contact with an anti-shock component – fortificanty and then strongly is kept on it even at reusable washing of cells by isotonic environments. These theoretical research were taken as a basis of a technique of determination of anti-shock properties of cryoresistance various the bioactive substances and biochemical connections by search, development and improvement of environments for a cryopreservation of sperm of different types of animals. When freezing sperm in nitrogen, an obligatory component of the cryoprotective environment is the native yolk of eggs containing many phospholipids and lipoproteins which at interaction with plasma membranes sperm modify them by increase of resistant and stability. Being adsorbed by lipophilic and hydrophilic sites of plasma membranes lipid complexes almost by 2-3 times thickencell membrane that gives stability to sperm to extreme temperature, osmotic, immunological, physical and chemical and mechanical to damages.
通过低温生物学、免疫学和生物物理学的方法,我们确定了在细胞温度冲击的机制中,渗透是起主导作用的,水通过它的瞬间运动是以破坏渗透压的平衡为代价的,这是细胞膜破坏的原因,剥夺了细胞质膜的半渗透性。研究了精子冷却时的强化机制,确定了强化剂在细胞表面积聚并形成疏水相,减缓了与“细胞-星期三”系统浓度梯度平衡相关的渗透作用。此外,可以确定的是,在细胞质膜与抗冲击成分接触后的2-5分钟内,保护层会在细胞质膜上形成,即使在等渗环境中重复洗涤细胞时,保护层也会牢固地保持在细胞质膜上。这些理论研究为通过寻找、开发和改善不同种类动物精子的低温保存环境来测定其抗低温冲击性能、各种生物活性物质和生物化学联系奠定了基础。当在氮气中冷冻精子时,冷冻保护环境的一个必要组成部分是含有许多磷脂和脂蛋白的卵子的天然蛋黄,这些磷脂和脂蛋白与质膜相互作用,精子通过增加抗性和稳定性来修饰它们。由于质膜的亲脂和亲水部位的脂质复合物几乎被2-3倍厚的细胞膜所吸附,使精子在极端温度、渗透、免疫、物理化学和机械损伤下保持稳定性。
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引用次数: 1
Effects of defaunation on rumen fermentation features, feed intake, digestibility and animal performance 降解对瘤胃发酵特性、采食量、消化率和动物生产性能的影响
Pub Date : 2016-07-17 DOI: 10.14196/sjas.v5i7.2204
Desta Tekle Gebru
The objective of the review was to provide compiled information on the effects of defaunation on rumen fermentation features, feed intake, digestibility and animal performance for development workers, academic user’s, researchers and other interested readers. Defaunation is the selective removal of protozoa from the rumen of animals. Based on the most of information reviewed defaunation decreases rumen pH, ruminal ammonia concentration, methane emissions, fibre carbohydrate digestibility and organic matter digestibility, and it increases total volatile fatty acids concentration, numbers of bacteria, feed conversion efficiency and weight gain but it did not affect dry matter intake.
本综述的目的是为开发工作者、学术用户、研究人员和其他感兴趣的读者提供有关defaunation对瘤胃发酵特征、采食量、消化率和动物生产性能的影响的汇编信息。清除是有选择性地从动物瘤胃中清除原生动物。根据所查阅的大部分资料,去臭降低了瘤胃pH、瘤胃氨浓度、甲烷排放量、纤维碳水化合物消化率和有机物消化率,提高了总挥发性脂肪酸浓度、细菌数量、饲料转化率和增重,但对干物质采食量没有影响。
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引用次数: 0
Effect of on farm supplementation of dried Sesbaniasesban (L.) leaf on performance of Abergelle rams 农场添加田菁干叶对小山羊生产性能的影响
Pub Date : 2016-07-17 DOI: 10.14196/sjas.v5i7.2202
Desta Tekle Gebru, Gebreslassie Gebru, H. Hagos, Shumiye Belay
The objectives of the study were to evaluate the effect of supplementation of dried Sesbaniasesban (L) leaf on supplement intake, body weight gain and cost benefit analysis of the feeding regimes for Abergellerams under farmer’s management system in Tigray region, northern Ethiopia. The experiment was carried using 21 yearling intact Abergelle rams with an  initial body weight of 22.83 + 3.11Kg (mean + standard deviation). The experiment comprised 90 days of feeding trial after 14 days of adaption period. Farmers having each 3 yearling intact Abergellerams with similar body weight were first purposively identified and then from the identified farmers 7 farmers were randomly selected. Randomized complete block design with 4 treatments and 7 replications was applied. The treatments were local feeding practices (T1), local feeding practices + 250 g -1 day dried Sesbaniasesbania leaf (T2) and local feeding practices + 300 g -1 daydried Sesbaniasesbania leaf (T3). The CP of dried Sesbaniasesban leaf was 23.34%. The DM, OM, CP, NDF, ADF and ADL intakes from the Sasbaniasesban leaf were significantly higher (p<0.0001) for T3 than T2. The average daily gain of Abergelle rams in T2 and T3 were significantly higher (p<0.0001) than T1 but similar in T2 and T3. The average daily gains were 28.98 g, 67.96 g and 75.71 g for T1, T2 and T3, respectively. Supplementation of dried Sesbaniasesba n leaf for Abergelle rams provide more profit compared to unsupplemented rams. Though T2 and T3 given comparable result in terms of body weight gain, the marginal rate of return suggested that supplementation of dried Sesbaniasesban leaf with 250 g -1 day than 300 g -1 day for Abergelle rams provided better economic gain and therefore, T2 is recommended as biological and economical sufficient supplementary regime for Abergelle rams.
本研究的目的是评估在埃塞俄比亚北部提格雷地区农民管理制度下补充干田葵(L)叶对补剂摄取量、体重增加和饲养制度的成本效益分析的影响。试验选用初始体重为22.83 + 3.11Kg(平均+标准差)的21只完整阿贝尔格公羊。预试期14 d,正试期90 d。首先有目的地挑选出3只体重相近的完整阿贝菌群的农户,然后从确定的农户中随机抽取7只。采用随机完全区组设计,4个处理,7个重复。处理分别为局部饲喂(T1)、局部饲喂+ 250 g -1 d干田菁叶(T2)和局部饲喂+ 300 g -1 d干田菁叶(T3)。干田菁叶CP为23.34%。T3处理叶片DM、OM、CP、NDF、ADF和ADL的采食量显著高于T2处理(p<0.0001)。T2和T3阶段阿贝尔格公羊的平均日增重显著高于T1 (p<0.0001),但T2和T3阶段基本相同。T1、T2和T3的平均日增重分别为28.98 g、67.96 g和75.71 g。与未补充的公羊相比,补充干田葵和叶片为阿贝尔格公羊提供了更多的利润。虽然T2和T3在增重方面具有可比性,但边际收益率表明,对阿伯吉公羊补充250 g -1 d的干田叶比300 g -1 d的经济收益更好,因此,推荐T2作为阿伯吉公羊生物和经济上足够的补充方案。
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引用次数: 0
Aspect of reproductive biology of the cassava croaker, Pseudotolithus senegalensis (valenciennes, 1833) of Ivory coast continental shelf 象牙海岸大陆架木薯鱼Pseudotolithus senegalensis (valenciennes, 1833)生殖生物学方面的研究
Pub Date : 2016-07-17 DOI: 10.14196/SJBS.V5I7.2250
S. Sylla, C. B. Tia, K. Kouakou, Amoin Céline Kouame, P. E. Kouamelan, B. C. Atsé
From January to December 2014, some aspects of Pseudotolithus senegalensis reproductive biology in Ivory coast were investigated and focused on sex ratio, gonadosomatic and liver-somatic index, size at first maturity, stages of gonadal development and fecundity. A total of 696 fish with body length of 12 to 36 cm standard length (SL) and body weight of 25. 2 to 675.1 g were used for this study. The sex ratio of P. senegalensis was 1: 0.5 (male to female). The females were more than the males (x 2 0,05 (1) = 51.36; p< 0,05). Males and females reach first maturity at 19.01 and 22.28 cm SL respectively. The monthly variations of the gonadosomatic index (GSI), hepatosomatic index (HIS) and the condition factor (K) indicated two periods of reproduction. The long period and the short period were respectively from March to June and from September to November. The hepatic reserves seem to be mobilized to ensure the energetic cost of the reproduction. The absolute Fecundity was ranged from 78612 to 140946 eggs and was positively correlated with standard length and weight. Fecundity-length and fecundity-weight relationships showed positive correlations. The fecundity-length and fecundity-weight relationships were determined by regression analysis with the regression equation F= 12.820xSL– 321.70 (r=0.99) for fecundity-length and F= 0.27xTW – 46.66 (r=0.98) for fecundity-weigth relationship.
2014年1 - 12月,对象牙海岸塞内加尔假石(Pseudotolithus senegalensis)生殖生物学的一些方面进行了研究,重点研究了性别比例、性腺和肝体指数、初成熟大小、性腺发育阶段和繁殖力。共696条鱼,体长为12至36厘米标准长度(SL),体重为25。2 ~ 675.1 g用于本研究。雌雄比为1:1 .5。女性多于男性(χ 2, 0.05 (1) = 51.36;p < 0 05)。雄性和雌性分别在19.01和22.28 cm SL达到首次成熟。性腺成体指数(GSI)、肝成体指数(HIS)和条件因子(K)的逐月变化反映了生殖的两个阶段。长周期为3 ~ 6月,短周期为9 ~ 11月。肝脏储备似乎被动员起来,以确保生殖的能量消耗。绝对繁殖力在78612 ~ 140946个卵之间,与标准体长和体重呈正相关。繁殖力长度与繁殖力重量呈正相关关系。对繁殖力-长度和繁殖力-重量关系进行回归分析,繁殖力-长度的回归方程为F= 12.820 xtw - 321.70 (r=0.99),繁殖力-重量的回归方程为F= 0.27xTW - 46.66 (r=0.98)。
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引用次数: 1
Reproductive biology of big-eye grunt Brachydeuterus Auritus in Ivory coast fishery (West Africa) 科特迪瓦(西非)渔业大眼鲷(brachyde子宫)的生殖生物学
Pub Date : 2016-05-15 DOI: 10.14196/SJBS.V5I5.2209
S. Sylla, T. T. Zan-Bi, K. J. Konan, C. B. Tia, J. Kabre, T. Koné
Reproductive biology of Big-eye grunt, Brachydeuterus auritus resulting from Ivory Coast marine fishery were studied by monthly sampling from August 2013 to July 2014. Samplings were carried out along the Ivorian littoral. A total of 731 fish with a body length of 8.0 to 22.7 cm folk length (LF) and body weight of 9.2 to 204.6 g were used for this study. According this study, the sex ratio was 1:0.82 (male to female). The sex ratio was significantly different from the expected theoretically 1: 1 distribution except June, September and December. Monthly gonadosomatic index and macroscopically determined gonad stages indicated that B. auritus spawned from February to July with a greater activity from February to May. The condition factor indicated that B. auritus were in good condition, females had higher condition factor than the males. Lengths at 50% maturity were similar with 12.93 cm LF for males and 12.71 cm LF for females. The coefficient b (3.02; 3.07 and 3.04 respectively for males, females and both sexes) of the length-weight relationship was reflecting isometry.
2013年8月至2014年7月,对科特迪瓦海洋渔业养殖的大眼咕噜鱼(bigeye grunt)进行了生殖生物学研究。沿着科特迪瓦海岸进行了采样。研究对象为体长8.0 ~ 22.7 cm、体重9.2 ~ 204.6 g的鱼731条。根据这项研究,男女性别比为1:0.82。除6月、9月和12月外,性别比例与理论预期的1:1分布有显著差异。月性腺指数和宏观测定性腺分期表明,2 - 7月为金螨产卵期,2 - 5月为活跃期。条件因子分析表明,金黄色小蠊状况良好,雌性条件因子高于雄性。50%成熟时体长相似,雄性体长12.93 cm,雌性体长12.71 cm。系数b (3.02;长重关系(男、女、两性)分别为3.07和3.04。
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引用次数: 6
Intestinal microbiota composition with probiotic potential of three species of the genus Chirostoma 三种Chirostoma属植物肠道菌群组成及益生菌潜力
Pub Date : 2016-05-15 DOI: 10.14196/sjas.v5i5.2225
M. D. Carmen, Javier Velasco Sarabia, Diego Alberto Retana Ortega, Ma. De los Angeles M. Peralta, M. Martínez, Gustavo Alejandro Rodríguez Montes de Oc, J. C. Reyes
The aim of this research was to identify the microbiota that was found in C, jordani, C. humboldtianum and C. estor to obtain those strains with probiotic capacity and can be used in Chirostoma sp. culture. Pre-adult stages were obtained for dissection and gain an intestinal tissue portion and get pure strains throughout consecutive reseeding in NHI and TSA agar plates. Then proceeded to the molecular identification with RNAr 16S sequencing gen and establish their probiotic capacity growing them in acid pH, bile salts, pathogen inhibition to Aeromonas hydrophila and Vibrio fluvialisin vitro and with antibiotics according to FAO (2006) and Balcazar et al . (2006) techniques. Results shown that analyzed Chirostoma sp. from intestinal tract were dominated by Proteobacteria, Actinobacteria and Firmicutes identifying 60 strains, 40% of them were from C. jordani ; 30% from C. humboldtianum and 20% of strains were from C. estor . The three analyzed species share some bacterial groups even if they were grown in different environmental conditions. The common species strains were Lactobacillus sp., L.crispatus , Bacillus subtilis , B. sp., Aeromonashydrophila, A. hydrophila caviae, Vibrio fluvialis and Pseudomonaluteola . The only bacterial species that resisted stress experiments, correspond to Bacillus sp. genus represented by Bacillus subtilis , Bacillus sp. and Bacillus laterodporus , so they are good probiotics candidates for culture of Chirostoma sp. genus.
本研究的目的是鉴定C、jordani、C. humboldtianum和C. estor中存在的微生物群,以获得具有益生菌能力的菌株,并可用于Chirostoma的培养。在NHI和TSA琼脂板上连续重新播种,获得成虫前阶段进行解剖,获得肠道组织部分,获得纯菌株。然后根据FAO(2006)和Balcazar等人的研究,用RNAr 16S测序基因进行分子鉴定,并确定它们在酸性pH、胆汁盐、体外对嗜水气单胞菌和流感弧菌的病原体抑制以及抗生素中培养的益生菌能力。(2006)技术。结果表明,所分析的肠道Chirostoma细菌以变形菌门、放线菌门和厚壁菌门为主,共鉴定60株,其中约40%为C. jordani;其中洪堡梭菌30%,斯托氏梭菌20%。这三种被分析的物种即使生长在不同的环境条件下,也有一些共同的细菌群。常见菌种有乳杆菌、葡萄球菌、枯草芽孢杆菌、芽孢杆菌、嗜水气单胞菌、嗜水气单胞菌、河流弧菌和假单胞菌。唯一抗胁迫实验的细菌为枯草芽孢杆菌属、芽孢杆菌属和后芽孢杆菌属,是培养Chirostoma属的良好益生菌候选菌。
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引用次数: 1
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Scientific Journal of Animal Science
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