A. Polevoy, A. Mykytiuk, L. Bozhko, E. Barsukova, K. Husieva
The moisture-temperature regime influences the nitrogen status of the soil and the microbiological processes of the transformation of nitrogen forms. Therefore, we assumed that ongoing climate change may affect the emission of nitrous oxide N2O, which is formed in the course of the transformation of nitrogen forms in the soil and is one of the most aggressive greenhouse gases that determine the global warming potential. To test this hypothesis and make a quantitative assessment of the impact of climate change on the transformation of nitrogen forms in the soil and N2O emissions from the agroecosystem soils, we used a complex model of greenhouse gas emissions from an agroecosystem soil that we developed and the RCP4.5 climate change scenario. The research was performed for the chernozem soils of Eastern Ukraine, with winter wheat being the cultivated crop. ‘Dry’ and ‘wet’ years typical for the period of 2021–2050 were selected among climate conditions in accordance with the climate change scenario. A ‘dry’ year was considered to be a year with a precipitation of 60% or less of the long-term average, a ‘wet’ year was a year with a precipitation of 130% or more of the long-term average for the studied period. The level of ammonification during the growing season for both ‘dry’ and partially ‘wet’ years was mainly controlled by temperature. At the beginning of the period, at negative temperatures below –3 °C ammonification and nitrification almost stopped, and at temperatures above –2 °C they slightly increased. Indices for the intensity of these processes increased with a rise in temperature from 0 °C to 10–11 °C. In the conditions of a ‘dry’ year an increment in temperature to the level of 22.4–27.8 °C caused a sharp increase in the rate of ammonification and caused a high level of the rate of nitrification. For the conditions of a ‘wet’ year with a sufficiently low (compared to a ‘dry’ year) temperature regime, a relatively significant rise in temperature with still quite good humidification increased the intensity of the ammonification process. The dependence of the intensity of the ammonification and nitrification processes on the moisture reserves in the soil is traced. The high level of moisture reserves in the arable layer at the beginning of the growing season in both ‘dry’ and ‘wet’ years formed anaerobic conditions in the upper layer of the soil. Against the background of rising temperatures, this called forth the denitrification process. N2O emission was 0.03–0.29 g N-N2O/ha•day due to the denitrification process in a ‘dry’ year, and 0.7–5.2 g N-N2O/ha•day in a ‘wet’ year. The highest level of N2O emission due to nitrification was observed in a ‘dry’ year in the middle of the vegetation period at high temperatures (22.7–27.8 °С) and amounted to 8.2–11.2 g N-N2O/ha•day. A decrease in soil moisture reserves during the second half of the growing season reduced the level of N2O emissions. Nitrification was the main process producing N2O. The
湿度-温度状况影响土壤的氮状况以及氮形态转化的微生物过程。因此，我们假设持续的气候变化可能会影响一氧化二氮的排放，这是在土壤中氮形态转化过程中形成的，是决定全球变暖潜力的最具侵略性的温室气体之一。为了检验这一假设，并对气候变化对土壤中氮形态转化和农业生态系统土壤N2O排放的影响进行定量评估，我们使用了我们开发的农业生态系统土地温室气体排放的复杂模型和RCP4.5气候变化情景。这项研究是针对乌克兰东部的黑钙土进行的，种植的作物是冬小麦根据气候变化情景，在气候条件中选择了2021年至2050年的典型干旱和潮湿年份。“干旱”年被认为是降水量为长期平均值60%或以下的年份，“湿润”年被视为降水量为研究期间长期平均值130%或以上的年份。在“干旱”和部分“湿润”年份的生长季节，氨化水平主要受温度控制。在这一时期开始时，在低于-3°C的负温度下，氨化和硝化几乎停止，而在高于-2°C的温度下，它们略有增加。这些过程的强度指数随着温度从0°C上升到10–11°C而增加。在“干旱”年份的条件下，温度升高到22.4–27.8°C的水平会导致氨化速率急剧增加，并导致高水平的硝化速率。对于温度足够低的“潮湿”年份（与“干燥”年份相比），相对显著的温度上升和相当好的加湿增加了氨化过程的强度。追踪了氨化和硝化过程的强度对土壤水分储量的依赖性。在“干旱”和“潮湿”年份的生长季节开始时，耕作层的高水分储量在土壤上层形成了厌氧条件。在温度升高的背景下，这就提出了脱氮过程。在“干旱”年份，由于脱氮过程，N2O排放量为0.03–0.29 g N-N2O/ha•天，在“潮湿”年份，N2O的排放量为0.7–5.2 g N-N2O/ha•天。在高温（22.7–27.8°С）下植被期中期的“干旱”年份，由于硝化作用，N2O排放量最高，达8.2–11.2 g N-N2O/ha•day。生长季节后半段土壤水分储量的减少降低了N2O的排放水平。硝化作用是产生N2O的主要过程。耕作层中水分储量大于55mm的N2O排放是由于反硝化作用造成的。温度升高会增加一氧化二氮的排放量。根据RCP4.5气候变化情景，评估了乌克兰东部“干旱”和“潮湿”年份的水分和温度条件对黑钙土氨化、硝化、反硝化和N2O排放过程的影响特点。
{"title":"Modelling of the impact of climate change on the transformation of nitrogen forms in the soil and N2O emissions from the agroecosystems of Eastern Ukraine","authors":"A. Polevoy, A. Mykytiuk, L. Bozhko, E. Barsukova, K. Husieva","doi":"10.15421/022325","DOIUrl":"https://doi.org/10.15421/022325","url":null,"abstract":"The moisture-temperature regime influences the nitrogen status of the soil and the microbiological processes of the transformation of nitrogen forms. Therefore, we assumed that ongoing climate change may affect the emission of nitrous oxide N2O, which is formed in the course of the transformation of nitrogen forms in the soil and is one of the most aggressive greenhouse gases that determine the global warming potential. To test this hypothesis and make a quantitative assessment of the impact of climate change on the transformation of nitrogen forms in the soil and N2O emissions from the agroecosystem soils, we used a complex model of greenhouse gas emissions from an agroecosystem soil that we developed and the RCP4.5 climate change scenario. The research was performed for the chernozem soils of Eastern Ukraine, with winter wheat being the cultivated crop. ‘Dry’ and ‘wet’ years typical for the period of 2021–2050 were selected among climate conditions in accordance with the climate change scenario. A ‘dry’ year was considered to be a year with a precipitation of 60% or less of the long-term average, a ‘wet’ year was a year with a precipitation of 130% or more of the long-term average for the studied period. The level of ammonification during the growing season for both ‘dry’ and partially ‘wet’ years was mainly controlled by temperature. At the beginning of the period, at negative temperatures below –3 °C ammonification and nitrification almost stopped, and at temperatures above –2 °C they slightly increased. Indices for the intensity of these processes increased with a rise in temperature from 0 °C to 10–11 °C. In the conditions of a ‘dry’ year an increment in temperature to the level of 22.4–27.8 °C caused a sharp increase in the rate of ammonification and caused a high level of the rate of nitrification. For the conditions of a ‘wet’ year with a sufficiently low (compared to a ‘dry’ year) temperature regime, a relatively significant rise in temperature with still quite good humidification increased the intensity of the ammonification process. The dependence of the intensity of the ammonification and nitrification processes on the moisture reserves in the soil is traced. The high level of moisture reserves in the arable layer at the beginning of the growing season in both ‘dry’ and ‘wet’ years formed anaerobic conditions in the upper layer of the soil. Against the background of rising temperatures, this called forth the denitrification process. N2O emission was 0.03–0.29 g N-N2O/ha•day due to the denitrification process in a ‘dry’ year, and 0.7–5.2 g N-N2O/ha•day in a ‘wet’ year. The highest level of N2O emission due to nitrification was observed in a ‘dry’ year in the middle of the vegetation period at high temperatures (22.7–27.8 °С) and amounted to 8.2–11.2 g N-N2O/ha•day. A decrease in soil moisture reserves during the second half of the growing season reduced the level of N2O emissions. Nitrification was the main process producing N2O. The ","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45177389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. V. Ukhovskyi, O. M. Romanov, O. M. Chechet, M. P. Sytiuk, L. Y. Korniienko, T. M. Tsarenko, M. L. Radzykhovskyi, A. P. Gerilovych
The article presents the results of a molecular genetic study of two isolates of the Pseudorabies virus that were isolated from pigs in Ukraine. Bioinformatic analysis of the gE gene fragment of Aujeszky's disease virus (Pseudorabies virus) isolates was carried out in order to determine the phylogenetic relationships and homology of nucleotide sequences. Fragments of the Aujeszky disease virus genome corresponding to the C-terminal region of the gE gene were selected for sequencing and further analysis. As a result of the conducted studies, it was demonstrated that the nucleotide sequences of the analyzed samples differ from each other by the presence of ACG insert in the tandem repeats region. Comparison of the studied sequences with the sequences of strains/isolates of the Aujeszky's disease virus found in Europe and Asia, presented in the GenBank database, indicates that such an insert is characteristic for the Min-A and HNJZ strains (position 1487 in the gE gene) isolated in Asia. Analysis of the homology of nucleotide sequences showed that the sequence of the gE gene fragment of sample No. 1 is 100% identical to the sequences of strains 89V87 and 00V72 isolated in Belgium. The homology of the nucleotide sequence of the gE gene fragment of sample No. 3 with strains 89V87 and 00V72 was 99.13%. In order to clarify the analyzed samples belonging to a particular genogroup (genetic cluster), a phylogenetic dendrogram was constructed. This demonstrates the phylogenetic relationships between strains/isolates of the Aujeszky's disease virus. It was found that the analyzed samples belong to the genetic cluster uniting European strains/isolates, and the studied isolates are most genetically close to strains 89V87 and 00V72.
{"title":"Molecular characterization and phylogenetic analysis of pseudorabies virus isolated from pigs in Ukraine","authors":"V. V. Ukhovskyi, O. M. Romanov, O. M. Chechet, M. P. Sytiuk, L. Y. Korniienko, T. M. Tsarenko, M. L. Radzykhovskyi, A. P. Gerilovych","doi":"10.15421/022327","DOIUrl":"https://doi.org/10.15421/022327","url":null,"abstract":"The article presents the results of a molecular genetic study of two isolates of the Pseudorabies virus that were isolated from pigs in Ukraine. Bioinformatic analysis of the gE gene fragment of Aujeszky's disease virus (Pseudorabies virus) isolates was carried out in order to determine the phylogenetic relationships and homology of nucleotide sequences. Fragments of the Aujeszky disease virus genome corresponding to the C-terminal region of the gE gene were selected for sequencing and further analysis. As a result of the conducted studies, it was demonstrated that the nucleotide sequences of the analyzed samples differ from each other by the presence of ACG insert in the tandem repeats region. Comparison of the studied sequences with the sequences of strains/isolates of the Aujeszky's disease virus found in Europe and Asia, presented in the GenBank database, indicates that such an insert is characteristic for the Min-A and HNJZ strains (position 1487 in the gE gene) isolated in Asia. Analysis of the homology of nucleotide sequences showed that the sequence of the gE gene fragment of sample No. 1 is 100% identical to the sequences of strains 89V87 and 00V72 isolated in Belgium. The homology of the nucleotide sequence of the gE gene fragment of sample No. 3 with strains 89V87 and 00V72 was 99.13%. In order to clarify the analyzed samples belonging to a particular genogroup (genetic cluster), a phylogenetic dendrogram was constructed. This demonstrates the phylogenetic relationships between strains/isolates of the Aujeszky's disease virus. It was found that the analyzed samples belong to the genetic cluster uniting European strains/isolates, and the studied isolates are most genetically close to strains 89V87 and 00V72.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136173836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We studied the effects of the newly synthesized biologically active compound potassium aminobenzene thiosulfonate on electrophysiological parameters of the embryos of weatherfish (Misgurnus fossіlis L.), in particular the dynamics of transmembrane potential (TMP) of the plasmatic membranes of the weatherfish and the activity of the membrane enzyme Na+/K+–ATPase during synchronous cleavage of blastomeres in early embryogenesis. A slight impairment of electrogenesis of the cellular membranes under the action of potassium aminobenzene thiosulfonate indicates changes in the permeability of plasmatic membrane and transport of electrogenic ions. This was related to the inhibition of biosynthetic processes in the first hours of the development of embryos, which led to 60.6 ± 2.6% decrease in the activity of membrane pump when subject to high (10–3 М) concentration of potassium aminobenzene thiosulfonate. Its activity further recovered to the level of the control only in 10–8 М concentration. Also, we conducted a comparative analysis of the effects potassium aminobenzenethiosulfonate had on the activity of the membrane pump of embryos in in vivo and in vitro experiments. We determined that the action of the examined compound depends on the presence of a correspondding concentration in the embryo incubation medium. To characterize the variability of changes in the activity of membrane pump of the embryos in the conditions of action of potassium aminobenzene thiosulfonate, we determined constants of semi-inhibition (І50) by linearization of the developed concentration-effect curves using Hill’s plot. To determine which factors contribute to the changes in the activity of membrane pump the most, namely, various concentrations of potassium p-aminobenzene thiosulfonate, duration of the development of embryos or other factors that had not been taken into account, we performed a dispersion analysis of how these factors affect the variability of the studied parameter. We determined that the extent of variability of the activity of the membrane pump is also determined by the effect of different concentrations of potassium p-aminobenzene thiosulfonate, and the factor of time of embryo development. The electronic-microscopic study of weatherfish’s blastomeres subject to potassium p-aminobenzene thiosulfonate revealed the changes in the ultrastructure of mitochondria, which led to inhibition of their matrix and electron transport chain, and therefore decrease the efficiency of ATP production and energy-dependent processes.
{"title":"Influence of potassium p-aminobenzene thiosulfate on the membrane potential and ATPase activity of the plasmatic membrane of the embryos of weatherfish (Misgurnus fossіlis)","authors":"О. Yaremkevych, V. Lubenets","doi":"10.15421/022322","DOIUrl":"https://doi.org/10.15421/022322","url":null,"abstract":"We studied the effects of the newly synthesized biologically active compound potassium aminobenzene thiosulfonate on electrophysiological parameters of the embryos of weatherfish (Misgurnus fossіlis L.), in particular the dynamics of transmembrane potential (TMP) of the plasmatic membranes of the weatherfish and the activity of the membrane enzyme Na+/K+–ATPase during synchronous cleavage of blastomeres in early embryogenesis. A slight impairment of electrogenesis of the cellular membranes under the action of potassium aminobenzene thiosulfonate indicates changes in the permeability of plasmatic membrane and transport of electrogenic ions. This was related to the inhibition of biosynthetic processes in the first hours of the development of embryos, which led to 60.6 ± 2.6% decrease in the activity of membrane pump when subject to high (10–3 М) concentration of potassium aminobenzene thiosulfonate. Its activity further recovered to the level of the control only in 10–8 М concentration. Also, we conducted a comparative analysis of the effects potassium aminobenzenethiosulfonate had on the activity of the membrane pump of embryos in in vivo and in vitro experiments. We determined that the action of the examined compound depends on the presence of a correspondding concentration in the embryo incubation medium. To characterize the variability of changes in the activity of membrane pump of the embryos in the conditions of action of potassium aminobenzene thiosulfonate, we determined constants of semi-inhibition (І50) by linearization of the developed concentration-effect curves using Hill’s plot. To determine which factors contribute to the changes in the activity of membrane pump the most, namely, various concentrations of potassium p-aminobenzene thiosulfonate, duration of the development of embryos or other factors that had not been taken into account, we performed a dispersion analysis of how these factors affect the variability of the studied parameter. We determined that the extent of variability of the activity of the membrane pump is also determined by the effect of different concentrations of potassium p-aminobenzene thiosulfonate, and the factor of time of embryo development. The electronic-microscopic study of weatherfish’s blastomeres subject to potassium p-aminobenzene thiosulfonate revealed the changes in the ultrastructure of mitochondria, which led to inhibition of their matrix and electron transport chain, and therefore decrease the efficiency of ATP production and energy-dependent processes.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41847758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Koreneva, O. Orobchenko, M. Romanko, N. Malova, R. Sachuk, B. Gutyj, M. L. Radzykhovskyi
Bromine content in fodder and water for laying chickens in Ukraine gradually increasing, thereby accumulating in the poultry production (eggs and meat): according to the data, intake of bromine with chicken eggs in 2020 has almost doubled, compared with 2016. Taking into account the ability of bromine to accumulate and the scales of consumption of poultry products, it is relevant to study the effects of high bromine concentration on the level of thyroid hormones and clinical-biochemical parameters of blood of white rats. At the laboratory of Toxicological Monitoring of the National Scientific Center the Institute of Experimental and Clinical Veterinary Medicine and the Department of Experimental Pharmacology and Toxicology of the State Institution V.Y. Danylevskyi Institute of Problems of Endocrine Pathology of the National Academy of Medical Sciences of Ukraine, we carried out studies on white outbred male rats (n = 144). The diet of experimental groups was supplemented with eggs and meat containing high bromine concentration. We determined increase in the coefficients of liver weight and decrease in the coefficients of lung weight in the experimental rats. Moreover, we determined changes in the biochemical blood profile, in particular: after egg consumption, there occurred 20.0–22.0% decrease in the enzymatic activity of alanine aminotransferase, 23.0–48.4% in the activity of aspartate aminotransferase, and on average 21.3% decrease in alkaline phosphatase, compared with the control; after meat consumption, the level of total proteins decreased by 8.6%, glucose by 12.2–14.5%, also there were 13.8–18.5% decrease in the activity of alanine aminotransferase and 12.1–83.0% increase in the activity of aspartate aminotransferase on the 28th day, and 23.2–35.3% decrease in the activity of alkaline phosphatase. After the intake of both poultry products, the blood serum of animals was observed to have decrease in the level of overall thyroxine and overall triiodothyronine (1.3 times on average, depending on the period of study). The results of the studies can help in prediction and timely alleviation of the negative impact of poultry products with high bromine content on the human organism.
{"title":"Influence of high-bromine poultry products on clinical-biochemical blood parameters of white rats","authors":"Y. Koreneva, O. Orobchenko, M. Romanko, N. Malova, R. Sachuk, B. Gutyj, M. L. Radzykhovskyi","doi":"10.15421/022319","DOIUrl":"https://doi.org/10.15421/022319","url":null,"abstract":"Bromine content in fodder and water for laying chickens in Ukraine gradually increasing, thereby accumulating in the poultry production (eggs and meat): according to the data, intake of bromine with chicken eggs in 2020 has almost doubled, compared with 2016. Taking into account the ability of bromine to accumulate and the scales of consumption of poultry products, it is relevant to study the effects of high bromine concentration on the level of thyroid hormones and clinical-biochemical parameters of blood of white rats. At the laboratory of Toxicological Monitoring of the National Scientific Center the Institute of Experimental and Clinical Veterinary Medicine and the Department of Experimental Pharmacology and Toxicology of the State Institution V.Y. Danylevskyi Institute of Problems of Endocrine Pathology of the National Academy of Medical Sciences of Ukraine, we carried out studies on white outbred male rats (n = 144). The diet of experimental groups was supplemented with eggs and meat containing high bromine concentration. We determined increase in the coefficients of liver weight and decrease in the coefficients of lung weight in the experimental rats. Moreover, we determined changes in the biochemical blood profile, in particular: after egg consumption, there occurred 20.0–22.0% decrease in the enzymatic activity of alanine aminotransferase, 23.0–48.4% in the activity of aspartate aminotransferase, and on average 21.3% decrease in alkaline phosphatase, compared with the control; after meat consumption, the level of total proteins decreased by 8.6%, glucose by 12.2–14.5%, also there were 13.8–18.5% decrease in the activity of alanine aminotransferase and 12.1–83.0% increase in the activity of aspartate aminotransferase on the 28th day, and 23.2–35.3% decrease in the activity of alkaline phosphatase. After the intake of both poultry products, the blood serum of animals was observed to have decrease in the level of overall thyroxine and overall triiodothyronine (1.3 times on average, depending on the period of study). The results of the studies can help in prediction and timely alleviation of the negative impact of poultry products with high bromine content on the human organism.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46966728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
М. І. Bashchenko, О. Boiko, О. F. Honchar, Y. Sotnichenko, Y. Lesyk, R. Iskra, B. Gutyj
Use of genetic specifics of pure breeding in animal husbandry allows cross breeders to produce herds that are completely of high breeding value, and also improve genetic potential of the best breeds. A relevant issue is determining the efficiency of crossbreeding of dairy breeds by analyzing the growth intensity of young bovine cattle, their reproductive ability and dairy productivity. We performed an analysis of experimental studies of using effective methods of selection, carried out a theoretical analysis, formulated hypotheses, used genealogical, biometrical and statistical methods, and assessed the productive properties of animals. We determined that the heifers from cross of the Ukrainian Red-Spotted cows with the sires of the Montbeliard breed – compared with the heifers from the Holstein bulls – had higher growth intensity and greater live weight during all the age periods. From birth until the age of one year, the Ukrainian Black-Spotted dairy heifers from the Holstein and Norwegian Red sires had no significant difference in weight. The heifers that resulted from cross of the Montbeliard and Ukrainian Red-Spotted dairy breeds had advantages over the purebred animals during all age periods, namely in the measurements of chest width, hook bone width, chest girth, ring metacarpal length. Body measurements of the replacement heifers, derived from the Norwegian Red and Holstein sires, varied within insignificant ranges depending on genotype, without significant difference. The animals were compact and proportionate; having received sufficient and complete diets, they had satisfactory weight categories. We determined that increase of the age of first insemination and first pregnancy was followed by decrease in the dairy productivity of cows. It ranged 0.021 to 0.064 in the bred heifers of different genotypes and breeds. There was observed an insignificant direct relationship between the abovementioned factors and the contents of fat and protein in milk. The shares of influence of age when the animals were first inseminated on milk yield accounted for 7.1% to 11.4%, 5.4–6.9 on fat content in milk, and 8.0–12.2% on protein fraction in milk. Crossbreeding can efficiently decrease the percentage of complicated deliveries and stillbirth rate, which in turn decreases the expenses for treatment of consequences of difficult calving, including the risk of decline in milk productivity and longer calving interval. However, there should be a careful approach to the selection of a breed, sire (preferably, its evaluation should be taken into account according to calving ease, especially when using the Montbeliard breed) and analysis of dams that are to be crossed with. Upgrading by mating to the Montbeliard and Norwegian breeds resulted in the crossbred cows that produced the greatest profit due to their heightened productivity. We should note that the estimated prices of extra products per cow give us grounds to state a positive effect from crossbreeding w
{"title":"Peculiarities of growth and further productivity of purebred and crossbred cows","authors":"М. І. Bashchenko, О. Boiko, О. F. Honchar, Y. Sotnichenko, Y. Lesyk, R. Iskra, B. Gutyj","doi":"10.15421/022318","DOIUrl":"https://doi.org/10.15421/022318","url":null,"abstract":"Use of genetic specifics of pure breeding in animal husbandry allows cross breeders to produce herds that are completely of high breeding value, and also improve genetic potential of the best breeds. A relevant issue is determining the efficiency of crossbreeding of dairy breeds by analyzing the growth intensity of young bovine cattle, their reproductive ability and dairy productivity. We performed an analysis of experimental studies of using effective methods of selection, carried out a theoretical analysis, formulated hypotheses, used genealogical, biometrical and statistical methods, and assessed the productive properties of animals. We determined that the heifers from cross of the Ukrainian Red-Spotted cows with the sires of the Montbeliard breed – compared with the heifers from the Holstein bulls – had higher growth intensity and greater live weight during all the age periods. From birth until the age of one year, the Ukrainian Black-Spotted dairy heifers from the Holstein and Norwegian Red sires had no significant difference in weight. The heifers that resulted from cross of the Montbeliard and Ukrainian Red-Spotted dairy breeds had advantages over the purebred animals during all age periods, namely in the measurements of chest width, hook bone width, chest girth, ring metacarpal length. Body measurements of the replacement heifers, derived from the Norwegian Red and Holstein sires, varied within insignificant ranges depending on genotype, without significant difference. The animals were compact and proportionate; having received sufficient and complete diets, they had satisfactory weight categories. We determined that increase of the age of first insemination and first pregnancy was followed by decrease in the dairy productivity of cows. It ranged 0.021 to 0.064 in the bred heifers of different genotypes and breeds. There was observed an insignificant direct relationship between the abovementioned factors and the contents of fat and protein in milk. The shares of influence of age when the animals were first inseminated on milk yield accounted for 7.1% to 11.4%, 5.4–6.9 on fat content in milk, and 8.0–12.2% on protein fraction in milk. Crossbreeding can efficiently decrease the percentage of complicated deliveries and stillbirth rate, which in turn decreases the expenses for treatment of consequences of difficult calving, including the risk of decline in milk productivity and longer calving interval. However, there should be a careful approach to the selection of a breed, sire (preferably, its evaluation should be taken into account according to calving ease, especially when using the Montbeliard breed) and analysis of dams that are to be crossed with. Upgrading by mating to the Montbeliard and Norwegian breeds resulted in the crossbred cows that produced the greatest profit due to their heightened productivity. We should note that the estimated prices of extra products per cow give us grounds to state a positive effect from crossbreeding w","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43559337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. I. Didenko, Y. Gaidar, D. Mylostiva, I. Klenina, А. A. Halinskyi, O. Petishko, O. Hrabovska, А. N. Halinska
Liver diseases represent one of the most common problems in gastroenterology. The liver, as the most important organ of metabolism, which plays a major role in anabolic and energy processes, takes part in the adaptive and compensatory reactions of the body under exogenous and endogenous adverse influences. Individual factors play a major role in the development of this disease, one of which is the duration of the effect of alcohol on the body. The duration of alcohol consumption affects the morpho-functional properties of the liver. When alcohol was given to the research animals, hypertrophic changes were seen in the linear dimensions of hepatocytes: in the 12th week of alcoholization, the nuclear area was 1.25 times larger, and the cytoplasmic area of hepatocytes was 1.16 times larger compared with the same indicators in the 6th week of alcoholization. Alcoholic hepatitis is characterized by protein-fatty degeneration, inflammatory lymphocytic infiltration, increase in the area of sinusoids and the size of hepatocytes, and, accordingly, the Vizotto coefficient (1.83 times in the 6th week and 2.10 times in the 12th week of the research). The alcohol consumption is accompanied by increase in the volume of the nucleus and cytoplasm of hepatocytes, decrease in the nuclear-cytoplasmic ratio with increase in pathohistological changes. With the lengthening of the terms of alcoholization, the number of binuclear hepatocytes decreased. Morphometric calculation of the number of fat cells per 100 hepatocytes showed that in the 12th week of the experiment, the number of fatty inclusions increased by 1.42 times, compared with 6 weeks of forced alcoholization. Fatty small- and large-droplet steatosis of hepatocytes was diffuse in nature. The duration of alcoholization also affects the liver vessels. The diameter of the central vein in the 6th week of the experiment was 1.52 times greater than in control rats, and 1.81 times in the 12th week of alcoholization. The bile ducts of rats of the experimental groups were also larger in diameter: by 37% after 6 weeks and 47% after 12 weeks of forced alcoholization. An increase in the area of the sinusoids and the diameter of the central vein indicates an impairment of the blood supply to the liver. The complexity of the structure of the liver and the diversity of its functions necessitate the use of a variety of diagnostic techniques and methodological approaches to assessing its activity in the normal condition, during a pathology. It will be promising to study the structure of the liver at the ultramicroscopic level of the effect of drugs on the treatment of alcoholic liver disease during different periods of alcoholization.
{"title":"Dynamics of pathomorphological changes in the liver of rats at different stages of experimental alcohol damage","authors":"V. I. Didenko, Y. Gaidar, D. Mylostiva, I. Klenina, А. A. Halinskyi, O. Petishko, O. Hrabovska, А. N. Halinska","doi":"10.15421/022320","DOIUrl":"https://doi.org/10.15421/022320","url":null,"abstract":"Liver diseases represent one of the most common problems in gastroenterology. The liver, as the most important organ of metabolism, which plays a major role in anabolic and energy processes, takes part in the adaptive and compensatory reactions of the body under exogenous and endogenous adverse influences. Individual factors play a major role in the development of this disease, one of which is the duration of the effect of alcohol on the body. The duration of alcohol consumption affects the morpho-functional properties of the liver. When alcohol was given to the research animals, hypertrophic changes were seen in the linear dimensions of hepatocytes: in the 12th week of alcoholization, the nuclear area was 1.25 times larger, and the cytoplasmic area of hepatocytes was 1.16 times larger compared with the same indicators in the 6th week of alcoholization. Alcoholic hepatitis is characterized by protein-fatty degeneration, inflammatory lymphocytic infiltration, increase in the area of sinusoids and the size of hepatocytes, and, accordingly, the Vizotto coefficient (1.83 times in the 6th week and 2.10 times in the 12th week of the research). The alcohol consumption is accompanied by increase in the volume of the nucleus and cytoplasm of hepatocytes, decrease in the nuclear-cytoplasmic ratio with increase in pathohistological changes. With the lengthening of the terms of alcoholization, the number of binuclear hepatocytes decreased. Morphometric calculation of the number of fat cells per 100 hepatocytes showed that in the 12th week of the experiment, the number of fatty inclusions increased by 1.42 times, compared with 6 weeks of forced alcoholization. Fatty small- and large-droplet steatosis of hepatocytes was diffuse in nature. The duration of alcoholization also affects the liver vessels. The diameter of the central vein in the 6th week of the experiment was 1.52 times greater than in control rats, and 1.81 times in the 12th week of alcoholization. The bile ducts of rats of the experimental groups were also larger in diameter: by 37% after 6 weeks and 47% after 12 weeks of forced alcoholization. An increase in the area of the sinusoids and the diameter of the central vein indicates an impairment of the blood supply to the liver. The complexity of the structure of the liver and the diversity of its functions necessitate the use of a variety of diagnostic techniques and methodological approaches to assessing its activity in the normal condition, during a pathology. It will be promising to study the structure of the liver at the ultramicroscopic level of the effect of drugs on the treatment of alcoholic liver disease during different periods of alcoholization.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44633919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Mylostуva, S. J. Farafonov, O. Puzniak, V. I. Stakhiv, V. Borshchenko, S. V. Tsisinska, S. V. Voloshin
Active forms of oxygen are formed in the course of the organism's vital activity in biochemical reactions. These forms, when the pro/antioxidant balance is disturbed, trigger a cascade of lipid peroxidation, which can be the cause of the development of various pathological conditions. To prevent the negative influence of lipid peroxidation products in the body, a powerful antioxidant system is activated. This system consists of an enzymatic and a non-enzymatic link. An important aspect of the normal functioning of this system is the provision of the body with important trace elements. A number of minerals are included in the active center of antioxidant enzymes or have an effect on the reactions of non-enzymatic antioxidants. Research was conducted on fattening bulls of the Ukrainian meat breed. During the monitoring of microelements in feed, it was found that the vast majority of farm feed was deficient in copper, selenium and manganese and for this reason the animals consumed an insufficient amount of these minerals. These data were confirmed by the low content of these trace elements in blood serum. The addition of inorganic salts of microelements to the basic diet led to an increase in the concentration of copper, manganese and selenium in the blood serum by 20.5%, 37.3% and 23.9%. The study of the content of lipid peroxidation products showed that during the 30 days of the experiment, the level of lipid hydroperoxide increased by 25.5%, diene conjugates by 22.8%, and malonic dialdehyde by 22.0%. This indicates that against the background of increased age-related metabolism in the body of young animals, the oxidation-reduction reactions that are a predictor of the start of peroxidation processes increase. It was also noted that with a deficiency of certain trace elements, the activity of both the enzymatic and non-enzymatic links of the antioxidant system was reduced. Thus, in 30 days, the level of catalase, superoxide dismutase, and glutathione peroxidase decreased by 9.4%, 15.3%, and 13.0%, respectively. During this time, the content of tocopherol and ceruloplasmin decreased by 16.8% and 9.8%. Additives also had a positive effect on the activity of the antioxidant system by increasing its components. Additives of trace elements had different effects on the activity of antioxidant enzymes. The greatest effect on the level of catalase and superoxide dismutase was observed when copper salts were added, when the increase of these enzymes was noted by 1.11 and 1.23 times, respectively. Accordingly, the level of glutathione peroxidase was the highest in animals that received additional selenium – 1.21 times. The addition of copper also had the greatest biological effect on the important non-enzymatic component of antioxidant protection – ceruloplasmin. Its level increased by 1.24 times under the action of copper sulfate. The level of tocopherol was higher under the action of manganese, when its concentration was 1.11 times higher than the control
{"title":"Antioxidant system of the body of young Ukrainian beef cattle under the action of microelements","authors":"D. Mylostуva, S. J. Farafonov, O. Puzniak, V. I. Stakhiv, V. Borshchenko, S. V. Tsisinska, S. V. Voloshin","doi":"10.15421/022316","DOIUrl":"https://doi.org/10.15421/022316","url":null,"abstract":"Active forms of oxygen are formed in the course of the organism's vital activity in biochemical reactions. These forms, when the pro/antioxidant balance is disturbed, trigger a cascade of lipid peroxidation, which can be the cause of the development of various pathological conditions. To prevent the negative influence of lipid peroxidation products in the body, a powerful antioxidant system is activated. This system consists of an enzymatic and a non-enzymatic link. An important aspect of the normal functioning of this system is the provision of the body with important trace elements. A number of minerals are included in the active center of antioxidant enzymes or have an effect on the reactions of non-enzymatic antioxidants. Research was conducted on fattening bulls of the Ukrainian meat breed. During the monitoring of microelements in feed, it was found that the vast majority of farm feed was deficient in copper, selenium and manganese and for this reason the animals consumed an insufficient amount of these minerals. These data were confirmed by the low content of these trace elements in blood serum. The addition of inorganic salts of microelements to the basic diet led to an increase in the concentration of copper, manganese and selenium in the blood serum by 20.5%, 37.3% and 23.9%. The study of the content of lipid peroxidation products showed that during the 30 days of the experiment, the level of lipid hydroperoxide increased by 25.5%, diene conjugates by 22.8%, and malonic dialdehyde by 22.0%. This indicates that against the background of increased age-related metabolism in the body of young animals, the oxidation-reduction reactions that are a predictor of the start of peroxidation processes increase. It was also noted that with a deficiency of certain trace elements, the activity of both the enzymatic and non-enzymatic links of the antioxidant system was reduced. Thus, in 30 days, the level of catalase, superoxide dismutase, and glutathione peroxidase decreased by 9.4%, 15.3%, and 13.0%, respectively. During this time, the content of tocopherol and ceruloplasmin decreased by 16.8% and 9.8%. Additives also had a positive effect on the activity of the antioxidant system by increasing its components. Additives of trace elements had different effects on the activity of antioxidant enzymes. The greatest effect on the level of catalase and superoxide dismutase was observed when copper salts were added, when the increase of these enzymes was noted by 1.11 and 1.23 times, respectively. Accordingly, the level of glutathione peroxidase was the highest in animals that received additional selenium – 1.21 times. The addition of copper also had the greatest biological effect on the important non-enzymatic component of antioxidant protection – ceruloplasmin. Its level increased by 1.24 times under the action of copper sulfate. The level of tocopherol was higher under the action of manganese, when its concentration was 1.11 times higher than the control","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42344429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Vashchenko, О. Zhukorskyi, A. Saenko, A. Khokhlov, S. Usenko, N. V. Kryhina, T. V. Sukhno, О. М. Tsereniuk
The growth and development of pigs is determined by their genotype and environmental conditions (primarily the level of feeding), however, the number of works aimed at studying the complex influence of genetic and non-genetic factors in their interaction is currently insufficient. The purpose of our work was to estimate the effect of the MC4R genotype, feeding level and interaction of these factors on growth and backfat thickness of crossbred pigs and to investigate the possibility of correcting the melanocortin-4-receptor gene polymorphism effect by adjusting the ration. Studies were conducted on 50 gilts obtained by crossing sows of the large white breed with landrace boars. Experimental pigs at the "Maxi 2010" farm were weighed at birth, then at the age of 28 days (at weaning) and at the age of 4, 6, 8 months. Fat thickness was measured at the age of 4, 6, 8 months. Genetic studies were conducted in a certified laboratory of Institute of Pig Breeding and Agroindustrial Production. Analysis of 50 blood samples revealed that this group of pigs had a sufficient level of polymorphism for research (Polymorphism Information Content was equil 0.35). The frequency of genotype distribution at the MC4R / SNP c.1426 G>A locus was 0.06 (AA) : 0.58 (GA) : 0.36 (GG). The type of feeding significantly influenced the live weight at the age of 4 months and the average daily gains of experimental pigs over the period of 28–120 days. Starting at the age of 6 months a significant effect of the interaction of organized factors (feeding + genotype) was recorded. At the age of 6 months, a significant influence of both the genotype and the level of feeding on the backfat thickness was established. Animals with the GG genotype receiving a restricted feed ration had significantly lower backfat thickness. At the age of 8 months, the difference in backfat thickness between the group with the GG genotype (restricted feed ration) and the AG genotype (high level feeding) reached a value of 12.9% (2.0 mm). Animals with the AG genotype had the lowest performance and the greatest fat thickness under feed limitation, which is important for raising young pigs for subsequent reproduction. Therefore, when selecting pigs to be used for further reproduction, the desired genotype is GG. In the future, it will be desirable to repeat the study on a larger number of pigs, so that the experiment involves a sufficient number of animals with the MC4R AA genotype for statistical processing.
{"title":"The influence of feeding level on the growth of pigs depending on their genotype","authors":"P. Vashchenko, О. Zhukorskyi, A. Saenko, A. Khokhlov, S. Usenko, N. V. Kryhina, T. V. Sukhno, О. М. Tsereniuk","doi":"10.15421/022317","DOIUrl":"https://doi.org/10.15421/022317","url":null,"abstract":"The growth and development of pigs is determined by their genotype and environmental conditions (primarily the level of feeding), however, the number of works aimed at studying the complex influence of genetic and non-genetic factors in their interaction is currently insufficient. The purpose of our work was to estimate the effect of the MC4R genotype, feeding level and interaction of these factors on growth and backfat thickness of crossbred pigs and to investigate the possibility of correcting the melanocortin-4-receptor gene polymorphism effect by adjusting the ration. Studies were conducted on 50 gilts obtained by crossing sows of the large white breed with landrace boars. Experimental pigs at the \"Maxi 2010\" farm were weighed at birth, then at the age of 28 days (at weaning) and at the age of 4, 6, 8 months. Fat thickness was measured at the age of 4, 6, 8 months. Genetic studies were conducted in a certified laboratory of Institute of Pig Breeding and Agroindustrial Production. Analysis of 50 blood samples revealed that this group of pigs had a sufficient level of polymorphism for research (Polymorphism Information Content was equil 0.35). The frequency of genotype distribution at the MC4R / SNP c.1426 G>A locus was 0.06 (AA) : 0.58 (GA) : 0.36 (GG). The type of feeding significantly influenced the live weight at the age of 4 months and the average daily gains of experimental pigs over the period of 28–120 days. Starting at the age of 6 months a significant effect of the interaction of organized factors (feeding + genotype) was recorded. At the age of 6 months, a significant influence of both the genotype and the level of feeding on the backfat thickness was established. Animals with the GG genotype receiving a restricted feed ration had significantly lower backfat thickness. At the age of 8 months, the difference in backfat thickness between the group with the GG genotype (restricted feed ration) and the AG genotype (high level feeding) reached a value of 12.9% (2.0 mm). Animals with the AG genotype had the lowest performance and the greatest fat thickness under feed limitation, which is important for raising young pigs for subsequent reproduction. Therefore, when selecting pigs to be used for further reproduction, the desired genotype is GG. In the future, it will be desirable to repeat the study on a larger number of pigs, so that the experiment involves a sufficient number of animals with the MC4R AA genotype for statistical processing.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46035505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Among the general effects of alcohol use, it has a negative effect on the bone system, so the development of prevention methods is becoming an increasingly urgent problem. The present study was aimed at evaluating the efficiency of the preventive complexes for the bone system in chronic alcohol intoxication. The study was conducted on 2-month-old female rats for 104 days. Chronic alcoholism in animals was simulated by replacing drinking water with an ethanol solution, the concentration of which was gradually increased from 8–25%. The prophylactic was administered by introducing into the diet a complex of vitamins (P, C, D) and minerals (Cu, Mg, Zn, Se, Mn), the main component of which was crushed oyster shells processed with citric acid. The second prophylactic complex was the clay mineral montmorillonite in combination with vitamins P, C, D. The biochemical markers of resorption (elastase and acid phosphatase activity), osteogenesis (calcium content, alkaline phosphatase activity) and the state of the antioxidant system (catalase, superoxide dismutase, glutathione reductase activity, malondialdehyde content) were determined in bones (jaws and femurs). Chronic alcohol consumption led to an increase in the degree of atrophy of the alveolar process, a decrease in femur bone density due to a decrease in the mineral component in bone tissue, and did not affect the state of the lumbar vertebrae. Chronic alcohol intoxication led to a decrease in the level of calcium in the blood serum of rats, and to a decrease in the alkaline phosphatase activity and the calcium content in the bone tissue of the jaws and femur against the background of increases in the activities of acid phosphatase and elastase. A significant decrease in the activity of the main antioxidant enzymes (superoxide dismutase, catalase and glutathione reductase) in bone tissue of animals against the background of an increase in the malondialdehyde content under conditions of chronic alcoholization has been established. The use of the complex with crushed oyster shells effectively prevented atrophy ofthe alveolar process in the jaws, did not affect the morphometric parameters in the femur and vertebrae, but normalized the activities of serum alkaline phosphatase and catalase, bone elastase, acid phosphatase, superoxide dismutase and glutathione reductase against the background of restoring the levels of calcium and malondialdehyde both in the serum and in the bone tissue of rats subjected to chronic alcoholization. Prophylactic administration of the vitamin complex with montmorillonite had a much weaker effect on the studied indicators. The obtained research results allow us to conclude that the osteoprotective and antioxidant efficiency of the complex with crushed oyster shells is more pronounced than that of montmorillonite under conditions of chronic alcohol intoxication.
{"title":"Prophylactic efficiency of the administration of vitamin, mineral and sorbent complexes on bone tissue in female rats against the background of chronic alcohol consumption","authors":"O. Makarenko, V. Kika, I. Khodakov, L. Khromagina","doi":"10.15421/022314","DOIUrl":"https://doi.org/10.15421/022314","url":null,"abstract":"Among the general effects of alcohol use, it has a negative effect on the bone system, so the development of prevention methods is becoming an increasingly urgent problem. The present study was aimed at evaluating the efficiency of the preventive complexes for the bone system in chronic alcohol intoxication. The study was conducted on 2-month-old female rats for 104 days. Chronic alcoholism in animals was simulated by replacing drinking water with an ethanol solution, the concentration of which was gradually increased from 8–25%. The prophylactic was administered by introducing into the diet a complex of vitamins (P, C, D) and minerals (Cu, Mg, Zn, Se, Mn), the main component of which was crushed oyster shells processed with citric acid. The second prophylactic complex was the clay mineral montmorillonite in combination with vitamins P, C, D. The biochemical markers of resorption (elastase and acid phosphatase activity), osteogenesis (calcium content, alkaline phosphatase activity) and the state of the antioxidant system (catalase, superoxide dismutase, glutathione reductase activity, malondialdehyde content) were determined in bones (jaws and femurs). Chronic alcohol consumption led to an increase in the degree of atrophy of the alveolar process, a decrease in femur bone density due to a decrease in the mineral component in bone tissue, and did not affect the state of the lumbar vertebrae. Chronic alcohol intoxication led to a decrease in the level of calcium in the blood serum of rats, and to a decrease in the alkaline phosphatase activity and the calcium content in the bone tissue of the jaws and femur against the background of increases in the activities of acid phosphatase and elastase. A significant decrease in the activity of the main antioxidant enzymes (superoxide dismutase, catalase and glutathione reductase) in bone tissue of animals against the background of an increase in the malondialdehyde content under conditions of chronic alcoholization has been established. The use of the complex with crushed oyster shells effectively prevented atrophy ofthe alveolar process in the jaws, did not affect the morphometric parameters in the femur and vertebrae, but normalized the activities of serum alkaline phosphatase and catalase, bone elastase, acid phosphatase, superoxide dismutase and glutathione reductase against the background of restoring the levels of calcium and malondialdehyde both in the serum and in the bone tissue of rats subjected to chronic alcoholization. Prophylactic administration of the vitamin complex with montmorillonite had a much weaker effect on the studied indicators. The obtained research results allow us to conclude that the osteoprotective and antioxidant efficiency of the complex with crushed oyster shells is more pronounced than that of montmorillonite under conditions of chronic alcohol intoxication.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67578523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adult stem cells, such as MSCs, spontaneously differentiate in vitro. This makes it difficult both to study this important cell type and to grow large numbers of MSCs for clinical use. While conventional cell cultivation methods cannot cope with this problem, nanostructured materials science offers hope. The effect of small-sized spherical nanoparticles based on orthovanadates of rare-earth elements activated by europium (GdYVO4:Eu3+ nanoparticles, diameter 1–2 nm) on cell-cell adhesion of rat bone marrow mesenchymal stem cells (rBM-MSCs) in vitro was studied using electrophoretic separation of proteins, immunofluorescence and confocal laser scanning microscopy. Our study revealed that rBM-MSCs treated with small-sized GdYVO4:Eu3+ nanoparticles had a significant impairment of intercellular adhesion in vitro. The pre-incubation of mesenchymal stem cells of rat bone marrow with GdYVO4:Eu3+ nanocrystals at a non-toxic concentration of 0.5 µg/mL during 1 hour of cultivation did not lead to significant changes in cell monolayer, the number of cells and the area of cell bodies did not change. However, the density of the monolayer and the area of the cell field decreased after the incubation. The incubation of cells with nanoparticles led to an increase in the area of the intercellular gate – a location of disruption of cell adhesion, compared to cells without nanoparticles in culture medium. The pre-incubation of rBM-MSCs with nanocrystals caused no changes in the content of total cadherins in the plasma membrane; a decrease in the content of cytoplasmic calreticulin and an increase in the content of surface calreticulin; a decrease in the content of free calcium in the cytoplasm, and an increase in protein-bound intercellular calcium and calcium in the extracellular space. The colocalization analysis revealed that the colocalization of calreticulins with cadherins on the outer surface of the plasma membrane of cells significantly increased after the incubation with GdYVO4:Eu3+ nanocrystals. The paper proposes a possible mechanism of reducing the degree of adhesion by nanocrystals. This study emphasizes the possibility of modulating MSCs adhesion using GdYVO4:Eu3+ nanoparticles. The development of new technologies capable of mitigating adhesion is crucial for the development of regenerative strategies using stem cells.
{"title":"The effect of GdYVO4:Eu3+ nanocrystals on the intercellular adhesion of mesenchymal stem cells in vitro","authors":"Y. H. Kot, K. Kot, N. Kavok, V. Klochkov","doi":"10.15421/022313","DOIUrl":"https://doi.org/10.15421/022313","url":null,"abstract":"Adult stem cells, such as MSCs, spontaneously differentiate in vitro. This makes it difficult both to study this important cell type and to grow large numbers of MSCs for clinical use. While conventional cell cultivation methods cannot cope with this problem, nanostructured materials science offers hope. The effect of small-sized spherical nanoparticles based on orthovanadates of rare-earth elements activated by europium (GdYVO4:Eu3+ nanoparticles, diameter 1–2 nm) on cell-cell adhesion of rat bone marrow mesenchymal stem cells (rBM-MSCs) in vitro was studied using electrophoretic separation of proteins, immunofluorescence and confocal laser scanning microscopy. Our study revealed that rBM-MSCs treated with small-sized GdYVO4:Eu3+ nanoparticles had a significant impairment of intercellular adhesion in vitro. The pre-incubation of mesenchymal stem cells of rat bone marrow with GdYVO4:Eu3+ nanocrystals at a non-toxic concentration of 0.5 µg/mL during 1 hour of cultivation did not lead to significant changes in cell monolayer, the number of cells and the area of cell bodies did not change. However, the density of the monolayer and the area of the cell field decreased after the incubation. The incubation of cells with nanoparticles led to an increase in the area of the intercellular gate – a location of disruption of cell adhesion, compared to cells without nanoparticles in culture medium. The pre-incubation of rBM-MSCs with nanocrystals caused no changes in the content of total cadherins in the plasma membrane; a decrease in the content of cytoplasmic calreticulin and an increase in the content of surface calreticulin; a decrease in the content of free calcium in the cytoplasm, and an increase in protein-bound intercellular calcium and calcium in the extracellular space. The colocalization analysis revealed that the colocalization of calreticulins with cadherins on the outer surface of the plasma membrane of cells significantly increased after the incubation with GdYVO4:Eu3+ nanocrystals. The paper proposes a possible mechanism of reducing the degree of adhesion by nanocrystals. This study emphasizes the possibility of modulating MSCs adhesion using GdYVO4:Eu3+ nanoparticles. The development of new technologies capable of mitigating adhesion is crucial for the development of regenerative strategies using stem cells.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44932543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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