M. Remezok, T. Kolombar, O. V. Parhomenko, V. Brygadyrenko
The global climate changes are causing an increase in the number and harmfulness of slugs. Deroceras agreste (Linnaeus, 1758) (Stylommatophora, Agriolimacidae) is a polyphagous phytophage that damages over 150 species of plants, including many vegetables, cultivated berries and grasses. Other than decrease in yield, slugs cause deterioration of consumer qualities of the products, promote infections of plants, and are intermediate hosts of some parasites of mammals and birds. Thus, slugs impose great losses on agricultural farming, and therefore the objective of our study was determining the variability of locomotor activity of D. agreste slugs in reaction to aromatic substances. We determined repellent or attractive effects of those substances for the purpose of further using the obtained data for plant protection. We tested 52 substances and their mixtures, which were conditionally divided into the following groups: chemical solvents, plant extracts, aromatizers, organic acids and synthetic cosmetic additives. Only dimethyl sulfoxide could be identified as an attractant. All the rest of the substances increased the speed of the slugs to various degrees, but had no significant effect on the direction of the animals’ movement. Gasoline increased the speed of the slugs’ movement by 3.20 times, xylene by 4.56. The most effective organic acids and aromatizers to increase the moving speed of slugs were avobenzone and formic acid: the first caused a 2.83-fold increase in the moving speed, the other a 3.16-fold increase. Only one of 13 aromatic substances changed the direction of the slugs’ movement during the experiment – β-ionone. As with the plant extracts, the highest effect on locomotor activity of slugs was exerted by tree bark of Quillaja saponaria (3.64-fold) and Aesculus hippocastanum extract (4.33-fold). Furthermore, together with Capsicum frutescens, they changed the direction the mollusks were moving in, and therefore could be used as repellents. Synthetic cosmetic additives hydrolyzed silk and chrysalide oil exerted the greatest effects on the lcomotor activity of slugs (3.16 and 3.20 times, respectively). A total of 78.6% of the slugs moved away from chrysalide oil, and thus this oil may be suggested as a repellent, as well as mousse de babassu and cocamidopropyl betaine (84.6% and 78.6%, respectively). Therefore, a large amount of the tested substances to one or another extent made the slugs move faster, but most of them did not alter the direction in which the slugs were moving.
{"title":"Influence of aromatic substances on locomotor activity of Deroceras agreste slugs","authors":"M. Remezok, T. Kolombar, O. V. Parhomenko, V. Brygadyrenko","doi":"10.15421/022232","DOIUrl":"https://doi.org/10.15421/022232","url":null,"abstract":"The global climate changes are causing an increase in the number and harmfulness of slugs. Deroceras agreste (Linnaeus, 1758) (Stylommatophora, Agriolimacidae) is a polyphagous phytophage that damages over 150 species of plants, including many vegetables, cultivated berries and grasses. Other than decrease in yield, slugs cause deterioration of consumer qualities of the products, promote infections of plants, and are intermediate hosts of some parasites of mammals and birds. Thus, slugs impose great losses on agricultural farming, and therefore the objective of our study was determining the variability of locomotor activity of D. agreste slugs in reaction to aromatic substances. We determined repellent or attractive effects of those substances for the purpose of further using the obtained data for plant protection. We tested 52 substances and their mixtures, which were conditionally divided into the following groups: chemical solvents, plant extracts, aromatizers, organic acids and synthetic cosmetic additives. Only dimethyl sulfoxide could be identified as an attractant. All the rest of the substances increased the speed of the slugs to various degrees, but had no significant effect on the direction of the animals’ movement. Gasoline increased the speed of the slugs’ movement by 3.20 times, xylene by 4.56. The most effective organic acids and aromatizers to increase the moving speed of slugs were avobenzone and formic acid: the first caused a 2.83-fold increase in the moving speed, the other a 3.16-fold increase. Only one of 13 aromatic substances changed the direction of the slugs’ movement during the experiment – β-ionone. As with the plant extracts, the highest effect on locomotor activity of slugs was exerted by tree bark of Quillaja saponaria (3.64-fold) and Aesculus hippocastanum extract (4.33-fold). Furthermore, together with Capsicum frutescens, they changed the direction the mollusks were moving in, and therefore could be used as repellents. Synthetic cosmetic additives hydrolyzed silk and chrysalide oil exerted the greatest effects on the lcomotor activity of slugs (3.16 and 3.20 times, respectively). A total of 78.6% of the slugs moved away from chrysalide oil, and thus this oil may be suggested as a repellent, as well as mousse de babassu and cocamidopropyl betaine (84.6% and 78.6%, respectively). Therefore, a large amount of the tested substances to one or another extent made the slugs move faster, but most of them did not alter the direction in which the slugs were moving.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44498009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Andreyeva, O. Stoyanov, G. Chebotaryova, R. Vastyanov, V. Kalashnikov, A. O. Stoyanov
Acquired stenosis of the spinal canal is a common problem in human and animal pathology. It is defined as a pathological condition that mainly develops as degeneration of the spine with cervical myelopathy development, which is a characteristic manifestation of this pathology. Pain both in the neck and upper back is the leading syndrome of this pathology. 65 human patients aged 20–65 years and 19 dogs weighing more than 20 kg were examined with the aim of comparative clinical and morphometric analysis of stenosis of the spinal canal. The age of the dogs was maximally converted to the age of a person. Computed tomography using the Pavlov-Torg index and the reserve space evaluation supplemented diagnosis of stenosis of the spinal canal. The mass proportion of stenotic changes of the spinal canal against the background of the degenerative-dystrophic process was 21 in males (n = 25) 84.0%, females – 28 persons (n = 40) 70.0%. Pain syndrome was the main complaint in all patients. Cervicalgia prevailed in 60 (92.3%), pain in the upper back was registered in 32 (49.2%) patients. The intensity of pain syndrome was equal to 3.1 ± 0.4 points. Motor deficiency in the form of paresis, mainly of the distal parts of the hands, was registered in 48 patients (73.8%), pyramidal symptoms were noted in the legs in 32 (49.2%) patients, pelvic reservoir dysfunction (7.0–10.8%), and sensory disorders were also found (24.0–36.9%). Such manifestations of cervical myelopathy progressed slowly. Pyramidal symptoms were more frequent and more pronounced in men. According to the computed tomography scan, degenerative changes of the spine were maximal at the level of the C6 vertebra with the maximum clinical correlation (neurological deficit, pain syndrome, etc.). In the course of the research, probable stenosis of the spinal canal was found in people on computed tomography images. In the 19 dogs observed weighing 20 kg or more the presence of pain syndrome was evaluated in all the animals (100.0%) as well as behaviour change (100.0%). During the examination, gait disorder was detected (18.0–94.7%); reaction to palpation of the neck area (15.0–78.9%); the habitus of animals experiencing pain, especially during neck movements (14.0–73.7%), body tension (12.0–63.1%), etc. Bony outgrowths of the edges of the vertebrae and intervertebral joints were found in almost all dogs of 18 (94.7%) large breeds compared to others. Narrowing of the intervertebral openings, deformation and sclerotic changes of the locking plates, thickening of the yellow and elongated ligaments occurred three to four times more in large breeds compared to dogs weighing less than 20 kg, and were absent in cats. Stenosis of the spinal canal was detected in 15 (78.9%) dogs. Therefore, acquired stenosis of the spinal canal probably develops after degenerative-dystrophic changes in the neck in 87.7% of people and in 78.9% of dogs with clinical manifestations of cervical myelopathy and with persistent pain syndrome
{"title":"Comparative clinical and morphometric investigations of cervical stenosis of the spinal canal in humans and dogs","authors":"T. Andreyeva, O. Stoyanov, G. Chebotaryova, R. Vastyanov, V. Kalashnikov, A. O. Stoyanov","doi":"10.15421/022239","DOIUrl":"https://doi.org/10.15421/022239","url":null,"abstract":"Acquired stenosis of the spinal canal is a common problem in human and animal pathology. It is defined as a pathological condition that mainly develops as degeneration of the spine with cervical myelopathy development, which is a characteristic manifestation of this pathology. Pain both in the neck and upper back is the leading syndrome of this pathology. 65 human patients aged 20–65 years and 19 dogs weighing more than 20 kg were examined with the aim of comparative clinical and morphometric analysis of stenosis of the spinal canal. The age of the dogs was maximally converted to the age of a person. Computed tomography using the Pavlov-Torg index and the reserve space evaluation supplemented diagnosis of stenosis of the spinal canal. The mass proportion of stenotic changes of the spinal canal against the background of the degenerative-dystrophic process was 21 in males (n = 25) 84.0%, females – 28 persons (n = 40) 70.0%. Pain syndrome was the main complaint in all patients. Cervicalgia prevailed in 60 (92.3%), pain in the upper back was registered in 32 (49.2%) patients. The intensity of pain syndrome was equal to 3.1 ± 0.4 points. Motor deficiency in the form of paresis, mainly of the distal parts of the hands, was registered in 48 patients (73.8%), pyramidal symptoms were noted in the legs in 32 (49.2%) patients, pelvic reservoir dysfunction (7.0–10.8%), and sensory disorders were also found (24.0–36.9%). Such manifestations of cervical myelopathy progressed slowly. Pyramidal symptoms were more frequent and more pronounced in men. According to the computed tomography scan, degenerative changes of the spine were maximal at the level of the C6 vertebra with the maximum clinical correlation (neurological deficit, pain syndrome, etc.). In the course of the research, probable stenosis of the spinal canal was found in people on computed tomography images. In the 19 dogs observed weighing 20 kg or more the presence of pain syndrome was evaluated in all the animals (100.0%) as well as behaviour change (100.0%). During the examination, gait disorder was detected (18.0–94.7%); reaction to palpation of the neck area (15.0–78.9%); the habitus of animals experiencing pain, especially during neck movements (14.0–73.7%), body tension (12.0–63.1%), etc. Bony outgrowths of the edges of the vertebrae and intervertebral joints were found in almost all dogs of 18 (94.7%) large breeds compared to others. Narrowing of the intervertebral openings, deformation and sclerotic changes of the locking plates, thickening of the yellow and elongated ligaments occurred three to four times more in large breeds compared to dogs weighing less than 20 kg, and were absent in cats. Stenosis of the spinal canal was detected in 15 (78.9%) dogs. Therefore, acquired stenosis of the spinal canal probably develops after degenerative-dystrophic changes in the neck in 87.7% of people and in 78.9% of dogs with clinical manifestations of cervical myelopathy and with persistent pain syndrome","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49369946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Slivinska, V. Yaremchuk, A. Shcherbatyy, B. Gutyj, H. Zinko
Hepatoses of laying hens are quite common in poultry farms as a result of improper practices of poultry maintenance consisting in excessive number of protein feeds for provision of high productivity. The objective of the study was preventive efficacy of Gep-A-Stress hepatoprotectors (carnitine hydrochloride, D L methionine, sorbitol, choline chloride, magnesium sulfate heptahydrate) and Hepasan VS (L-carnitine hydrochloride, sorbitol, choline chloride, magnesium sulfate hepahydrate, betaine hydrochloride, L-arginin) against hepatosis of laying hens. To determine the efficacy of Hep-A-Stres and Hepasan-VS hepatoprotectors during production tests (n = 4,500), we monitored the parameters of survival rate (the final number of individuals as percentage of the initial number) and egg productivity of laying hens. We determined that after 30 days of using the hepatoprotectors, the content of overall protein in blood serum of laying hens of the first and the second experimental groups decreased by 21.4% and 18.9% compared with the parameters prior to providing the hepatoprotectors and by 26.3% and 23.3% compared with the control group after receiving the drug. The urea contents in blood serum increased by 19.0% and 10.5%. Compared with the control, the activity of alanine aminotransferase decreased by 43.7% and 24.1% in the first experimental group and by 23.4% and 14.9% in the second. The activity of aspartate aminotransferase in blood serum of the experimental groups decreased by 10.7%. The cholesterol concentration decreased by 50% and 58.3%. The content of triaglycerols decreased by 24.1% and 8.9% respectively. The concentrations of high-density lipoproteins after 30 days of the experiment decreased by 33.3% and 77.8% respectively, the content of low-density lipoproteins decreased by 61.3% and 40.4% and 42.3%. Population maintenance equaled respectively 97.1%, 98.3% and 98.1%. At the end of the experiment, the egg productivity of laying hens of the first and second groups increased by 4% and 3.6% compared with the control. Therefore, intake of the hepaprotectors by laying hens stimulated their metabolism, positively influenced the blood parameters, survival and egg productivity. The conducted studies confirm the benefits of using hepatoprotectors for the prohylaxis of hepatosis of hens.
{"title":"Efficacy of hepatoprotectors in prophylaxis of hepatosis of laying hens","authors":"L. Slivinska, V. Yaremchuk, A. Shcherbatyy, B. Gutyj, H. Zinko","doi":"10.15421/022237","DOIUrl":"https://doi.org/10.15421/022237","url":null,"abstract":"Hepatoses of laying hens are quite common in poultry farms as a result of improper practices of poultry maintenance consisting in excessive number of protein feeds for provision of high productivity. The objective of the study was preventive efficacy of Gep-A-Stress hepatoprotectors (carnitine hydrochloride, D L methionine, sorbitol, choline chloride, magnesium sulfate heptahydrate) and Hepasan VS (L-carnitine hydrochloride, sorbitol, choline chloride, magnesium sulfate hepahydrate, betaine hydrochloride, L-arginin) against hepatosis of laying hens. To determine the efficacy of Hep-A-Stres and Hepasan-VS hepatoprotectors during production tests (n = 4,500), we monitored the parameters of survival rate (the final number of individuals as percentage of the initial number) and egg productivity of laying hens. We determined that after 30 days of using the hepatoprotectors, the content of overall protein in blood serum of laying hens of the first and the second experimental groups decreased by 21.4% and 18.9% compared with the parameters prior to providing the hepatoprotectors and by 26.3% and 23.3% compared with the control group after receiving the drug. The urea contents in blood serum increased by 19.0% and 10.5%. Compared with the control, the activity of alanine aminotransferase decreased by 43.7% and 24.1% in the first experimental group and by 23.4% and 14.9% in the second. The activity of aspartate aminotransferase in blood serum of the experimental groups decreased by 10.7%. The cholesterol concentration decreased by 50% and 58.3%. The content of triaglycerols decreased by 24.1% and 8.9% respectively. The concentrations of high-density lipoproteins after 30 days of the experiment decreased by 33.3% and 77.8% respectively, the content of low-density lipoproteins decreased by 61.3% and 40.4% and 42.3%. Population maintenance equaled respectively 97.1%, 98.3% and 98.1%. At the end of the experiment, the egg productivity of laying hens of the first and second groups increased by 4% and 3.6% compared with the control. Therefore, intake of the hepaprotectors by laying hens stimulated their metabolism, positively influenced the blood parameters, survival and egg productivity. The conducted studies confirm the benefits of using hepatoprotectors for the prohylaxis of hepatosis of hens.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44952675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article covers two clinical cases of domestic cats suffering from a mediastinal form of lymphoma and provides their cytological, immunohistochemical, and pathohistological verification. The lymphoma immunophenotyping method using the B-cell markers CD79a and CD3 was used to identify T-cells. The mediastinal type of lymphoma in cats is usually rarely registered and statistically, the number of cases does not exceed 20%, which is consistent with our observations. Shortness of breath, difficulty with swallowing, lack of appetite, cyanotic mucous membranes, lymphadenomegaly of the mediastinal lymph nodes and effusion in the chest cavity were found in the clinically ill cats. An X-ray examination of the chest cavity revealed an increased contrast in the mediastinal area and displacement of the lungs towards the spine. A complete blood count revealed lymphopenia. Cytological smears of the chest cavity transudates revealed mostly monomorphic lymphoblasts with large rounded hyperchromic nuclei and narrow cytoplasm stained in light blue. The karyoplasm was somewhat granular, the nucleoli were visible, and the mitoses were atypical and numerous. The cytological picture in biopsy samples of mediastinal lymph nodes was characterized by a monomorphic population of lymphoblasts with an eccentrically located nucleus and moderately basophilic karyoplasm. The cell cytoplasm was vacuolated in some places, with presence of atypical mitotic figures. Most of the cells had two nuclei. Diffuse tumoral lesions of the chest cavity tissues, focal tumorous lesions of the lung parenchyma, and lymphadenopathy of the mediastinal lymph nodes were found during the autopsy in the first case. The second clinical case demonstrated the massive lymphadenopathy of the mediastinal lymph nodes. Histologically, a diffuse lesion by a monomorphic population of lymphoblasts was revealed in preparations from the soft tissues of the chest wall. The lymphoblasts’ insignificant cytoplasm, with nuclei from round to elliptical shape, infiltrated the chest wall’s soft tissues. It was found immunohistochemically that the blast cells showed reactivity to CD79a, and were mostly negative to CD3. This indicates the development of a large diffuse B-cell lymphoma. The immunohistochemical picture of lymph node necropsies from another cat was somewhat similar. However, it was also characterized by lymphoblasts with eccentrically placed nuclei, increased number of cells with mitotic figures, somewhat intensive CD3 antibodies expression (especially in the paracortical area of lymph nodes), and significant CD79a marker expression.
{"title":"Pathohistological features of mediastinal lymphoma in domestic cats","authors":"K. Oriekhova, O. Shchebentovska","doi":"10.15421/022241","DOIUrl":"https://doi.org/10.15421/022241","url":null,"abstract":"The article covers two clinical cases of domestic cats suffering from a mediastinal form of lymphoma and provides their cytological, immunohistochemical, and pathohistological verification. The lymphoma immunophenotyping method using the B-cell markers CD79a and CD3 was used to identify T-cells. The mediastinal type of lymphoma in cats is usually rarely registered and statistically, the number of cases does not exceed 20%, which is consistent with our observations. Shortness of breath, difficulty with swallowing, lack of appetite, cyanotic mucous membranes, lymphadenomegaly of the mediastinal lymph nodes and effusion in the chest cavity were found in the clinically ill cats. An X-ray examination of the chest cavity revealed an increased contrast in the mediastinal area and displacement of the lungs towards the spine. A complete blood count revealed lymphopenia. Cytological smears of the chest cavity transudates revealed mostly monomorphic lymphoblasts with large rounded hyperchromic nuclei and narrow cytoplasm stained in light blue. The karyoplasm was somewhat granular, the nucleoli were visible, and the mitoses were atypical and numerous. The cytological picture in biopsy samples of mediastinal lymph nodes was characterized by a monomorphic population of lymphoblasts with an eccentrically located nucleus and moderately basophilic karyoplasm. The cell cytoplasm was vacuolated in some places, with presence of atypical mitotic figures. Most of the cells had two nuclei. Diffuse tumoral lesions of the chest cavity tissues, focal tumorous lesions of the lung parenchyma, and lymphadenopathy of the mediastinal lymph nodes were found during the autopsy in the first case. The second clinical case demonstrated the massive lymphadenopathy of the mediastinal lymph nodes. Histologically, a diffuse lesion by a monomorphic population of lymphoblasts was revealed in preparations from the soft tissues of the chest wall. The lymphoblasts’ insignificant cytoplasm, with nuclei from round to elliptical shape, infiltrated the chest wall’s soft tissues. It was found immunohistochemically that the blast cells showed reactivity to CD79a, and were mostly negative to CD3. This indicates the development of a large diffuse B-cell lymphoma. The immunohistochemical picture of lymph node necropsies from another cat was somewhat similar. However, it was also characterized by lymphoblasts with eccentrically placed nuclei, increased number of cells with mitotic figures, somewhat intensive CD3 antibodies expression (especially in the paracortical area of lymph nodes), and significant CD79a marker expression.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49476339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Research on studying the features of 0.01%, 0.05%, 0.10% and 0.50% epimutagen Triton-X-305 impact at the cellular level, which means the way of identifying the viability of pollen in plants of the first generation, and cytological analysis of chromosomal aberrations in the cells of the primary meristem of germinal roots during germination of bread winter wheat seeds have been conducted. Research in this direction allows us to significantly improve the monitoring of same type of substances in the environment and to forecast the nature of their action at the DNA-level. Genotypes Podolyanka and Spivanka created by Ukrainian selection and varieties of French selection Altigo, Courtot, Lyrik, Flamenko have been studied. These genotypes were selected in order to characterize possible genotype-mutagenic interactions for a compound of complex hereditary pattern for a range of concentrations with maximum contrast taking into account the possible high site-specific effect. The main purpose of this research was to identify the specificity of impact of the agent Triton X-305 at the cellular level and identify parameters fully showing the effect of this substance on the subsequent induction of biodiversity and the enhancement of hereditary variability at the cellular level. Such indicators as pollen sterility effected by various concentrations, the total frequency of chromosomal rearrangements, the spectrum of chromosomal rearrangements, including fragments and double fragments, single and double bridges, micronuclei and lagging chromosomes have been investigated. The ratio of fragments to bridges as an indicator of the active factor nature, the number of cells with two or more rearrangements as an indicator of genetic toxicity of this substance has been established. As a result, a significantly weaker effect of the epimutagen on the overall frequency of chromosomal rearrangement has been shown unlike other factors of a mutagenic nature, at the same time changing the ratio of the obtained aberrations in favour of micronuclei and lagging chromosomes. In addition, other parameters are determined as more meaningful for identifying a specific agent of impact, and the differences in concentrations are less contrasting. There is also a less significantly decreasing fertility, however, this parameter is highly dependent on the source material. In the future, we intend to assess the variability, primarily of a hereditary nature, by the way of a visual analysis of the obtained material in subsequent generations, as well as through biochemical analysis, yield qualities analysis and morphometry of the obtained material.
{"title":"Occurrence of cytogenetic effects under the action of epimutagen in winter wheat","authors":"V. Beiko, M. Nazarenko","doi":"10.15421/022238","DOIUrl":"https://doi.org/10.15421/022238","url":null,"abstract":"Research on studying the features of 0.01%, 0.05%, 0.10% and 0.50% epimutagen Triton-X-305 impact at the cellular level, which means the way of identifying the viability of pollen in plants of the first generation, and cytological analysis of chromosomal aberrations in the cells of the primary meristem of germinal roots during germination of bread winter wheat seeds have been conducted. Research in this direction allows us to significantly improve the monitoring of same type of substances in the environment and to forecast the nature of their action at the DNA-level. Genotypes Podolyanka and Spivanka created by Ukrainian selection and varieties of French selection Altigo, Courtot, Lyrik, Flamenko have been studied. These genotypes were selected in order to characterize possible genotype-mutagenic interactions for a compound of complex hereditary pattern for a range of concentrations with maximum contrast taking into account the possible high site-specific effect. The main purpose of this research was to identify the specificity of impact of the agent Triton X-305 at the cellular level and identify parameters fully showing the effect of this substance on the subsequent induction of biodiversity and the enhancement of hereditary variability at the cellular level. Such indicators as pollen sterility effected by various concentrations, the total frequency of chromosomal rearrangements, the spectrum of chromosomal rearrangements, including fragments and double fragments, single and double bridges, micronuclei and lagging chromosomes have been investigated. The ratio of fragments to bridges as an indicator of the active factor nature, the number of cells with two or more rearrangements as an indicator of genetic toxicity of this substance has been established. As a result, a significantly weaker effect of the epimutagen on the overall frequency of chromosomal rearrangement has been shown unlike other factors of a mutagenic nature, at the same time changing the ratio of the obtained aberrations in favour of micronuclei and lagging chromosomes. In addition, other parameters are determined as more meaningful for identifying a specific agent of impact, and the differences in concentrations are less contrasting. There is also a less significantly decreasing fertility, however, this parameter is highly dependent on the source material. In the future, we intend to assess the variability, primarily of a hereditary nature, by the way of a visual analysis of the obtained material in subsequent generations, as well as through biochemical analysis, yield qualities analysis and morphometry of the obtained material.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44948321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Kovpak, O. Kovpak, O. A. Valchuk, Y. Zhuk, S. Derkach
Producing embryos in vitro is an important technology used to improve the genetic potential of cattle and perfect the programs of their breeding. Regardless of the way they are produced, all embryos that had not been used for transplantation to recipients must be conserved. Because of significantly increased interest in the problem of cryoconservation of embryos, both coming from scientists and businesses, there are emerging new commercial environments that allow the facilitation of cryoconservation and the increase in the embryo survival. Oocyte-cumulus complexes obtained from the ovaries of slaughtered clinically healthy cows matured in 22–24 h in in vitro conditions. The oocytes were co-cultured with spermatozoids in Fertilization medium, and the obtained zygotes were cultured in Culture medium with Sodium-Pyruvate for 4 or 7 days up to the stage of morula or blastocyste, respectively. For the vitrification of cow embryos, we used a commercial kit for the vitrification of human embryos, having compared the duration of equilibration. According to the results of the studies, we observed high efficiency of cryoconservation of cow embryos using the commercial kit for vitrification of human embryos. The results revealed the significant effect of equilibration on survival and further development of embryos. In addition, we described the dependence of development stage of cattle embryo on the duration of the contact of embryo with equilibration solution. Therefore, optimal time of contact of cattle embryos at the morula stage with equilibration solution was 12 minutes. On the 24th h after thawing, 46.7 ± 3.3% of the embryos were observed to undergo blastulation, and on 48th h, this parameter increased to 96.7 ± 3.3%, which corresponded to the parameters in the group of embryos that had not been subjected to cryoconservation. In the conditions of further cultivation, the percentage of blastocystes that hatched in the experimental group was no different from that of the control. At the same time, the highest efficiency of vitrification of blastocystes of cows was seen after the contact with the equilibration solution for 15 min, since the percentage of hatched blastocystes was the same as in the control group. Therefore, using the commercial kit for vitrification of human embryos is beneficial, for it promotes the parameters of cow embryos after vitrification/thawing that are similar to such of intact embryos (without freezing). The data we analyzed and presented in the paper could help to increase the efficiency of cryoconservation of cattle embryos for both scientific and commercial purposes.
{"title":"Specifics of vitrification of in vitro-produced cattle embyos at various development stages","authors":"V. Kovpak, O. Kovpak, O. A. Valchuk, Y. Zhuk, S. Derkach","doi":"10.15421/022234","DOIUrl":"https://doi.org/10.15421/022234","url":null,"abstract":"Producing embryos in vitro is an important technology used to improve the genetic potential of cattle and perfect the programs of their breeding. Regardless of the way they are produced, all embryos that had not been used for transplantation to recipients must be conserved. Because of significantly increased interest in the problem of cryoconservation of embryos, both coming from scientists and businesses, there are emerging new commercial environments that allow the facilitation of cryoconservation and the increase in the embryo survival. Oocyte-cumulus complexes obtained from the ovaries of slaughtered clinically healthy cows matured in 22–24 h in in vitro conditions. The oocytes were co-cultured with spermatozoids in Fertilization medium, and the obtained zygotes were cultured in Culture medium with Sodium-Pyruvate for 4 or 7 days up to the stage of morula or blastocyste, respectively. For the vitrification of cow embryos, we used a commercial kit for the vitrification of human embryos, having compared the duration of equilibration. According to the results of the studies, we observed high efficiency of cryoconservation of cow embryos using the commercial kit for vitrification of human embryos. The results revealed the significant effect of equilibration on survival and further development of embryos. In addition, we described the dependence of development stage of cattle embryo on the duration of the contact of embryo with equilibration solution. Therefore, optimal time of contact of cattle embryos at the morula stage with equilibration solution was 12 minutes. On the 24th h after thawing, 46.7 ± 3.3% of the embryos were observed to undergo blastulation, and on 48th h, this parameter increased to 96.7 ± 3.3%, which corresponded to the parameters in the group of embryos that had not been subjected to cryoconservation. In the conditions of further cultivation, the percentage of blastocystes that hatched in the experimental group was no different from that of the control. At the same time, the highest efficiency of vitrification of blastocystes of cows was seen after the contact with the equilibration solution for 15 min, since the percentage of hatched blastocystes was the same as in the control group. Therefore, using the commercial kit for vitrification of human embryos is beneficial, for it promotes the parameters of cow embryos after vitrification/thawing that are similar to such of intact embryos (without freezing). The data we analyzed and presented in the paper could help to increase the efficiency of cryoconservation of cattle embryos for both scientific and commercial purposes.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45201799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pulmonary fibrosis is one of the commonest forms of interstitial lung diseases with poorly studied methods of its treatment in both human and veterinary medicines. Therefore, this paper focused on seeking alternative methods of its diagnostics and treatment. The article provides the results of the study of bronchoalveolar lavage fluid of rats with experimental lung fibrosis and influence of transplanted allogeneic mesenchymal stem cells of the bone marrow on stimulation of regenerative processes in damaged lung tissues. The studies were conducted on female Wistar rats with pulmonary fibrosis modeled using single transthoracic injection of solution of bleomycin hydrochloride. For the purpose of treatment, we used allogeneic mesenchymal stem cells introduced by various methods and the traditional treatment. We determined that best normalization of the parameters of the studied brochoalveolar lavage occurred in animals that received mesenchymal stem cells. The most active repair processes were in the experimental group that received the mesenchymal stem cells directly to the lung tissue. The animals that received intravenous injection of mesenchymal stemm cells were observed to have lower clinical parameters of the brochoalveolar lavage, but still better than such in the group treated traditionally. The lowest parameters were in animals that received the traditional treatment; they were greater than the phisological parameters, but significantly exceeded them in animals of the control group, indicating presence of inflammatory process in the lung tissue. The conducted cytological assays of the samples of the brochoalveolar lavage revealed that experimental animals with experimental pulmonary fibrosis had development of macrophage and lymphocytic reactions under the influence of transplanted mesenchymal stemm cells. We observed no atypical cells in all the experimental groups. This allows us to draw a conclusion that using stem cells by various methods of transplantation does not stimulate the onset of negative reactons (formation of atypical cells, metastatic processes, etc). Thus, the results of the study of the influence of transplanted mesenchymal stem cells demonstrate that in the conditions of experimental pulmonary fibrosis, the activity of regenerative processes in pathologically altered lung tissue may be an effective method of treatment of animals with this kind of pathology.
{"title":"Effects of transplanted mesenchymal stem cells on repair of the lung tissue of rats with experimental pulmonary fibrosis","authors":"Y. Surtaieva, A. Mazurkevich, R. Bokotko","doi":"10.15421/022240","DOIUrl":"https://doi.org/10.15421/022240","url":null,"abstract":"Pulmonary fibrosis is one of the commonest forms of interstitial lung diseases with poorly studied methods of its treatment in both human and veterinary medicines. Therefore, this paper focused on seeking alternative methods of its diagnostics and treatment. The article provides the results of the study of bronchoalveolar lavage fluid of rats with experimental lung fibrosis and influence of transplanted allogeneic mesenchymal stem cells of the bone marrow on stimulation of regenerative processes in damaged lung tissues. The studies were conducted on female Wistar rats with pulmonary fibrosis modeled using single transthoracic injection of solution of bleomycin hydrochloride. For the purpose of treatment, we used allogeneic mesenchymal stem cells introduced by various methods and the traditional treatment. We determined that best normalization of the parameters of the studied brochoalveolar lavage occurred in animals that received mesenchymal stem cells. The most active repair processes were in the experimental group that received the mesenchymal stem cells directly to the lung tissue. The animals that received intravenous injection of mesenchymal stemm cells were observed to have lower clinical parameters of the brochoalveolar lavage, but still better than such in the group treated traditionally. The lowest parameters were in animals that received the traditional treatment; they were greater than the phisological parameters, but significantly exceeded them in animals of the control group, indicating presence of inflammatory process in the lung tissue. The conducted cytological assays of the samples of the brochoalveolar lavage revealed that experimental animals with experimental pulmonary fibrosis had development of macrophage and lymphocytic reactions under the influence of transplanted mesenchymal stemm cells. We observed no atypical cells in all the experimental groups. This allows us to draw a conclusion that using stem cells by various methods of transplantation does not stimulate the onset of negative reactons (formation of atypical cells, metastatic processes, etc). Thus, the results of the study of the influence of transplanted mesenchymal stem cells demonstrate that in the conditions of experimental pulmonary fibrosis, the activity of regenerative processes in pathologically altered lung tissue may be an effective method of treatment of animals with this kind of pathology.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42304004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Sukhno, P. Vashchenko, A. Saenko, O. Zhukorskyi, O. M. Tserenyuk, N. V. Kryhina
The purpose of our work was to study the polymorphism of genes associated with disease resistance and to search for their associations with productive traits in the population of the Ukrainian Large White pigs. For this study, 50 pigs were used, observations and measurements were carried out at the age from birth to 180 days. Genetic studies were carried out in a certified laboratory of the Institute of Pig Breeding and Agroindustrial Production. In the study of fucosyltransferase 1 and solute carrier family 11 member 1 genes, polymorphism was found in three of the five analyzed loci. In the Ukrainian Large White subpopulation of pigs the informativeness of these gene polymorphisms was at the optimal level for associative analysis, Polymorphism Information Content was greater than 0.3 in two loci. A sufficiently high level of Polymorphism Information Content indicates the value of this breed to preserve the biodiversity of pigs. The distribution of genotypes at some loci of the solute carrier family 11 member 1 gene was characterized by a deviation from the theoretically expected one due to the increase in the frequency of the heterozygous genotype. There was also a statistically confirmed deviation of the genotypes’ distribution from the normal and polymorphism fucosyltransferase 1 gene, but in this case in the direction of increasing the frequency of both homozygous variants. These results indicate the presence of a certain selection pressure on the mentioned polymorphisms and their possible impact on productive traits. The influence of solute carrier family 11 member 1 gene polymorphism on the weight of pigs at the age of 120 and 180 days, the average daily gain recorded in the period 28–120 days and from birth to 180 days, as well as on the backfat thickness, was established. The preferred genotype is TT, which can be used in breeding to obtain more productive animals with increased disease resistance, but in the selection of animals at this locus, it is necessary to control the backfat thickness and prevent breeding of pigs that may worsen this trait.
{"title":"Association of Fut1 and Slc11a1 gene polymorphisms with productivity traits of Large White pigs","authors":"V. Sukhno, P. Vashchenko, A. Saenko, O. Zhukorskyi, O. M. Tserenyuk, N. V. Kryhina","doi":"10.15421/022229","DOIUrl":"https://doi.org/10.15421/022229","url":null,"abstract":"The purpose of our work was to study the polymorphism of genes associated with disease resistance and to search for their associations with productive traits in the population of the Ukrainian Large White pigs. For this study, 50 pigs were used, observations and measurements were carried out at the age from birth to 180 days. Genetic studies were carried out in a certified laboratory of the Institute of Pig Breeding and Agroindustrial Production. In the study of fucosyltransferase 1 and solute carrier family 11 member 1 genes, polymorphism was found in three of the five analyzed loci. In the Ukrainian Large White subpopulation of pigs the informativeness of these gene polymorphisms was at the optimal level for associative analysis, Polymorphism Information Content was greater than 0.3 in two loci. A sufficiently high level of Polymorphism Information Content indicates the value of this breed to preserve the biodiversity of pigs. The distribution of genotypes at some loci of the solute carrier family 11 member 1 gene was characterized by a deviation from the theoretically expected one due to the increase in the frequency of the heterozygous genotype. There was also a statistically confirmed deviation of the genotypes’ distribution from the normal and polymorphism fucosyltransferase 1 gene, but in this case in the direction of increasing the frequency of both homozygous variants. These results indicate the presence of a certain selection pressure on the mentioned polymorphisms and their possible impact on productive traits. The influence of solute carrier family 11 member 1 gene polymorphism on the weight of pigs at the age of 120 and 180 days, the average daily gain recorded in the period 28–120 days and from birth to 180 days, as well as on the backfat thickness, was established. The preferred genotype is TT, which can be used in breeding to obtain more productive animals with increased disease resistance, but in the selection of animals at this locus, it is necessary to control the backfat thickness and prevent breeding of pigs that may worsen this trait.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48387837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Langraf, R. Babosová, K. Petrovičová, J. Schlarmannová, V. Brygadyrenko
Database systems store data (big data) for various areas dealing with finance (banking, insurance) and are also an essential part of corporate firms. In the field of biology, however, not much attention has been paid to database systems, with the exception of genetics (RNA, DNA) and human protein. Therefore data storage and subsequent implementation is insufficient for this field. The current situation in the field of data use for the assessment of biological relationships and trends is conditioned by constantly changing requirements, while data stored in simple databases used in the field of biology cannot respond operatively to these changes. In the recent period, developments in technology in the field of histology caused an increase in biological information stored in databases with which database technology did not deal. We proposed a new database for histology with designed data types (data format) in database program Microsoft SQL Server Management Studio. In order that the information to support identification of biological trends and regularities is relevant, the data must be provided in real time and in the required format at the strategic, tactical and operational levels. We set the data type according to the needs of our database, we used numeric (smallint,numbers, float), text string (nvarchar, varchar) and date. To select, insert, modify and delete data, we used Structured Query Language (SQL), which is currently the most widely used language in relational databases. Our results represent a new database for information about histology, focusing on histological structures in systems of animals. The structure and relational relations of the histology database will help in analysis of big data, the objective of which was to find relations between histological structures in species and the diversity of habitats in which species live. In addition to big data, the successful estimation of biological relationships and trends also requires the rapid accuracy of scientists who derive key information from the data. A properly functioning database for meta-analyses, data warehousing, and data mining includes, in addition to technological aspects, planning, design, implementation, management, and implementation.
数据库系统为处理金融(银行、保险)的各个领域存储数据(大数据)，也是公司的重要组成部分。然而，在生物学领域，除了遗传学(RNA, DNA)和人类蛋白质之外，对数据库系统的关注并不多。因此，该领域的数据存储和后续实现是不够的。用于评估生物关系和趋势的数据使用领域的现状受到不断变化的需求的制约，而储存在生物领域使用的简单数据库中的数据不能有效地对这些变化作出反应。近年来，组织学领域的技术发展导致数据库中存储的生物信息增加，而数据库技术无法处理这些信息。我们在数据库程序Microsoft SQL Server Management Studio中提出了一个新的组织学数据库，并设计了数据类型(数据格式)。为了使支持查明生物趋势和规律的信息具有相关性，必须在战略、战术和作战各级以所需格式实时提供数据。我们根据数据库的需要设置数据类型，我们使用了数字(smallint,numbers, float)，文本字符串(nvarchar, varchar)和日期。为了选择、插入、修改和删除数据，我们使用了结构化查询语言(SQL)，这是目前关系数据库中使用最广泛的语言。我们的结果代表了一个关于组织学信息的新数据库，专注于动物系统的组织学结构。组织数据库的结构和关系关系将有助于大数据分析，其目的是发现物种的组织结构与物种生活的栖息地多样性之间的关系。除了大数据之外，成功估计生物关系和趋势还需要科学家从数据中获取关键信息的快速准确性。一个用于元分析、数据仓库和数据挖掘的功能正常的数据库，除了技术方面，还包括规划、设计、实现、管理和实现。
{"title":"Storing and structuring big data in histological research (vertebrates) using a relational database in SQL","authors":"V. Langraf, R. Babosová, K. Petrovičová, J. Schlarmannová, V. Brygadyrenko","doi":"10.15421/022226","DOIUrl":"https://doi.org/10.15421/022226","url":null,"abstract":"Database systems store data (big data) for various areas dealing with finance (banking, insurance) and are also an essential part of corporate firms. In the field of biology, however, not much attention has been paid to database systems, with the exception of genetics (RNA, DNA) and human protein. Therefore data storage and subsequent implementation is insufficient for this field. The current situation in the field of data use for the assessment of biological relationships and trends is conditioned by constantly changing requirements, while data stored in simple databases used in the field of biology cannot respond operatively to these changes. In the recent period, developments in technology in the field of histology caused an increase in biological information stored in databases with which database technology did not deal. We proposed a new database for histology with designed data types (data format) in database program Microsoft SQL Server Management Studio. In order that the information to support identification of biological trends and regularities is relevant, the data must be provided in real time and in the required format at the strategic, tactical and operational levels. We set the data type according to the needs of our database, we used numeric (smallint,numbers, float), text string (nvarchar, varchar) and date. To select, insert, modify and delete data, we used Structured Query Language (SQL), which is currently the most widely used language in relational databases. Our results represent a new database for information about histology, focusing on histological structures in systems of animals. The structure and relational relations of the histology database will help in analysis of big data, the objective of which was to find relations between histological structures in species and the diversity of habitats in which species live. In addition to big data, the successful estimation of biological relationships and trends also requires the rapid accuracy of scientists who derive key information from the data. A properly functioning database for meta-analyses, data warehousing, and data mining includes, in addition to technological aspects, planning, design, implementation, management, and implementation.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45375558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Radzykhovskyi, I. Sokulskiy, O. Dyshkant, A. Antoniuk, B. Gutyj, R. Sachuk
The immune system unites the organs and the tissues that protect the organism against genetically alien cells or substances entering the organism from the environment. Canine parvovirus is an etiologic agent of hemorrhagic gastroenteritis and causes a significant problem for veterinary medicine due to high level of morbidity and mortality, mostly among dogs, because of fast progression without immune-complement response. In this study, based on the results of our clinical, virological, histological, histochemical and morphological assays, we determined the pathogenetic role of parvovirus in sick dogs experimentally infected per os, specifically with isolated canine parvovirus (Antaeus) with titer of infectious activity equaling 3.80 ± 0.008 lg TCID50/cm, cultivated on heterological cell cultures. This allowed us to clarify, add to and generalize the data on the pathogenesis of the disease and determine pathohistological and histochemical changes in the immunogenesis, since the studied virus expresses immune-suppressive properties, leading to ruination of the locomotor ability of the organism and fast lethal outcome. The study of pathomorphological changes was carried out using pathoanatomical and histologic methods. Pathoanatomical material from the autopsy of puppies aged 45 days was fixed in 10% aqueous solution of neutral formaline and embedded in paraffin. Having parvoviral infection, dogs experience pathomorphologic changes in immune-complement organs, indicating inhibition of the immunogenesis function during an infectious disease of viral etiology. In the immunogenesis organs of puppies with the experimental reconstruction of parvoviral enteritis, we microscopically determined the following: edema of the cortex and medulla, disorganization of thymic corpuscules, and impairment of processes of differentiation of lymphocytes in the cortex and medulla of the thymic lobules; edema and large areas of accumulation of hemosiderin in the spleen as a result of breakdown of large amount of erythrocytes; acute inflammatory hyperemia of the parenchyma, swelling of sinuses, serous and serous-hemorrhagic lymphadenitis in lymph nodes. The complex of histologic changes in the immune protection organs, which we found in the conditions of experimental reconstruction of parvoviral infection, can be considered a distinct criterion for pathomorphologic differentiation diagnostics of parvoviral enteritis in dogs.
{"title":"Experimental study of tropism of cultivated canine parvovirus in the immunogenesis organs of puppies","authors":"M. Radzykhovskyi, I. Sokulskiy, O. Dyshkant, A. Antoniuk, B. Gutyj, R. Sachuk","doi":"10.15421/022231","DOIUrl":"https://doi.org/10.15421/022231","url":null,"abstract":"The immune system unites the organs and the tissues that protect the organism against genetically alien cells or substances entering the organism from the environment. Canine parvovirus is an etiologic agent of hemorrhagic gastroenteritis and causes a significant problem for veterinary medicine due to high level of morbidity and mortality, mostly among dogs, because of fast progression without immune-complement response. In this study, based on the results of our clinical, virological, histological, histochemical and morphological assays, we determined the pathogenetic role of parvovirus in sick dogs experimentally infected per os, specifically with isolated canine parvovirus (Antaeus) with titer of infectious activity equaling 3.80 ± 0.008 lg TCID50/cm, cultivated on heterological cell cultures. This allowed us to clarify, add to and generalize the data on the pathogenesis of the disease and determine pathohistological and histochemical changes in the immunogenesis, since the studied virus expresses immune-suppressive properties, leading to ruination of the locomotor ability of the organism and fast lethal outcome. The study of pathomorphological changes was carried out using pathoanatomical and histologic methods. Pathoanatomical material from the autopsy of puppies aged 45 days was fixed in 10% aqueous solution of neutral formaline and embedded in paraffin. Having parvoviral infection, dogs experience pathomorphologic changes in immune-complement organs, indicating inhibition of the immunogenesis function during an infectious disease of viral etiology. In the immunogenesis organs of puppies with the experimental reconstruction of parvoviral enteritis, we microscopically determined the following: edema of the cortex and medulla, disorganization of thymic corpuscules, and impairment of processes of differentiation of lymphocytes in the cortex and medulla of the thymic lobules; edema and large areas of accumulation of hemosiderin in the spleen as a result of breakdown of large amount of erythrocytes; acute inflammatory hyperemia of the parenchyma, swelling of sinuses, serous and serous-hemorrhagic lymphadenitis in lymph nodes. The complex of histologic changes in the immune protection organs, which we found in the conditions of experimental reconstruction of parvoviral infection, can be considered a distinct criterion for pathomorphologic differentiation diagnostics of parvoviral enteritis in dogs.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49556637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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