B. Gutyj, T. Martyshuk, V. Parchenko, A. Kaplaushenko, I. Bushueva, I. Hariv, Y. P. Bilash, V. Brygadyrenko, Y. I. Turko, M. L. Radzykhovskyi
The balance between anti- and prooxidants in animal organisms in general and in each cell in particular is responsible for the regulation of many metabolic processes that provide immunocompetence, growth, development and protection of animals from oxidative stress, related to inflow of cadmium and plumbum. Therefore, the objective of our study was the influence of a liposomal drug based on interferon and milk thistle (Silybum marianum (L.) Gaertn.) on the antioxidative status of the organism of bulls in the conditions of cadmium and plumbum loading. The experiments were performed on six-months-old Black Motley dairy cattle. The fodders in the farm were determined to contain high levels of plumbum and cadmium. The liposomal drug Lipointersyl inhibited the lipid peroxidation processes in the bulls. The drug components promoted the decrease in the level of intermediate and end products of lipid peroxidation, in particular 22% decrease in the level of diene conjugates and 20% decrease in TBA-active products. Intramuscular injection of the liposomal drug to bulls of the experimental group strengthened the antioxidant protection of their organism. On the 30th day of the experiment, blood from experimental group animals was seen to have a 9.8% increase in reduced glutathione. Assay of the enzymatic link of the glutathione system revealed that the activity of glutathione peroxidase and glutathione reductase in the blood of the animals that had been injected the liposomal drug Lipointensyl had increased by 24.0% and 27.7% respectively by the 30th day of the experiment. The experiments conducted on young cattle demonstrated that intermuscularly injected the Lipointensyl liposomal drug – against the background of cadmium and plumbum loading – promoted the activation of the glutathione system of antioxidant protection as a result of increase in the activity of its enzymatic and non-enzymatic links. The study of catalase and superoxide dismutase activities revealed that on the 30th and 40th days of the experiment, the activity of those enzymes varied within the physiological norms. Therefore, the analyzed Silybum marianum-based liposomal drug has antioxidant properties, it is recommended for young cattle in the conditions of contamination with heavy metals in order to prevent the development of oxidative stress.
{"title":"Effect of liposomal drug based on interferon and extract from Silybum marianum on antioxidative status of bulls against the background of contamination of fodders by cadmium and plumbum","authors":"B. Gutyj, T. Martyshuk, V. Parchenko, A. Kaplaushenko, I. Bushueva, I. Hariv, Y. P. Bilash, V. Brygadyrenko, Y. I. Turko, M. L. Radzykhovskyi","doi":"10.15421/022255","DOIUrl":"https://doi.org/10.15421/022255","url":null,"abstract":"The balance between anti- and prooxidants in animal organisms in general and in each cell in particular is responsible for the regulation of many metabolic processes that provide immunocompetence, growth, development and protection of animals from oxidative stress, related to inflow of cadmium and plumbum. Therefore, the objective of our study was the influence of a liposomal drug based on interferon and milk thistle (Silybum marianum (L.) Gaertn.) on the antioxidative status of the organism of bulls in the conditions of cadmium and plumbum loading. The experiments were performed on six-months-old Black Motley dairy cattle. The fodders in the farm were determined to contain high levels of plumbum and cadmium. The liposomal drug Lipointersyl inhibited the lipid peroxidation processes in the bulls. The drug components promoted the decrease in the level of intermediate and end products of lipid peroxidation, in particular 22% decrease in the level of diene conjugates and 20% decrease in TBA-active products. Intramuscular injection of the liposomal drug to bulls of the experimental group strengthened the antioxidant protection of their organism. On the 30th day of the experiment, blood from experimental group animals was seen to have a 9.8% increase in reduced glutathione. Assay of the enzymatic link of the glutathione system revealed that the activity of glutathione peroxidase and glutathione reductase in the blood of the animals that had been injected the liposomal drug Lipointensyl had increased by 24.0% and 27.7% respectively by the 30th day of the experiment. The experiments conducted on young cattle demonstrated that intermuscularly injected the Lipointensyl liposomal drug – against the background of cadmium and plumbum loading – promoted the activation of the glutathione system of antioxidant protection as a result of increase in the activity of its enzymatic and non-enzymatic links. The study of catalase and superoxide dismutase activities revealed that on the 30th and 40th days of the experiment, the activity of those enzymes varied within the physiological norms. Therefore, the analyzed Silybum marianum-based liposomal drug has antioxidant properties, it is recommended for young cattle in the conditions of contamination with heavy metals in order to prevent the development of oxidative stress.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44058147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
О. Zhukorskyi, О. М. Tsereniuk, P. Vashchenko, A. Khokhlov, Y. Chereuta, О. V. Akimov, N. V. Kryhina
The reproductive performance of sows largely determines the efficiency of the entire pig farming industry. The purpose of our work is the evaluation of polymorphism of the ryanodine receptor gene and its impact on the reproductive traits of sows of the Welsh breed of pigs. For this study, 148 pigs of the Welsh breed were used. The reproductive traits of sows were evaluated in two adjacent generations. We conducted a comprehensive assessment of the reproductive ability of sows using the SIRQS (selection index of reproductive qualities of sows), determined phenotypic consolidation coefficients and assessed the genetic potential of the animals’ productivity. The polymorphism of the RYR1 gene was determined using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP). Data processing was performed using single-factor analysis of variance (ANOVA). Polymorphism of the ryanodine receptor gene in sows of the Welsh pig breed was evaluated. 8.0% of the animals were identified as carriers of the mutant allele of the RYR1 gene. However, no homozygous RYR1-nn animals were found. Pigs of the maternal generation carrying the homozygous NN genotype had better reproductive performance in all indicators. Sows that were carriers of the mutant allele were characterized by lower values of the genetic productivity potential compared with the entire estimated population for all productive traits. Sows which were free of the mutant allele of the RYR1 gene were characterized by large values of the SIRQS index. The values of the coefficients of phenotypic consolidation of the number of live born piglets in sows without the mutant allele were lower than in sows with the mutant allele n. Better performance of sows free of the mutant allele of the RYR1 gene was established over sows carrying it in all evaluated traits of reproductive capacity (for different traits P ranged from 0.021 to 1.0*10–4), except for number of piglets born alive per sow in the daughter generation. Sows with the NN genotype had better selection index values by 15.7% in the maternal generation and by 10.2% in the daughter generation. In order to increase the reproductive ability of sows in the studied population of Welsh pigs and achieve similar results in other herds of this breed, animals free from the mutant allele of the RYR1 gene should be selected for further reproduction in the process of breeding, while on the contrary, carriers of this gene should be gradually eliminated from the herd. To carry out breeding work, further research is needed on the entire population of Welsh pigs for the RYR1 gene.
{"title":"The effect of the ryanodine receptor gene on the reproductive traits of Welsh sows","authors":"О. Zhukorskyi, О. М. Tsereniuk, P. Vashchenko, A. Khokhlov, Y. Chereuta, О. V. Akimov, N. V. Kryhina","doi":"10.15421/022248","DOIUrl":"https://doi.org/10.15421/022248","url":null,"abstract":"The reproductive performance of sows largely determines the efficiency of the entire pig farming industry. The purpose of our work is the evaluation of polymorphism of the ryanodine receptor gene and its impact on the reproductive traits of sows of the Welsh breed of pigs. For this study, 148 pigs of the Welsh breed were used. The reproductive traits of sows were evaluated in two adjacent generations. We conducted a comprehensive assessment of the reproductive ability of sows using the SIRQS (selection index of reproductive qualities of sows), determined phenotypic consolidation coefficients and assessed the genetic potential of the animals’ productivity. The polymorphism of the RYR1 gene was determined using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP). Data processing was performed using single-factor analysis of variance (ANOVA). Polymorphism of the ryanodine receptor gene in sows of the Welsh pig breed was evaluated. 8.0% of the animals were identified as carriers of the mutant allele of the RYR1 gene. However, no homozygous RYR1-nn animals were found. Pigs of the maternal generation carrying the homozygous NN genotype had better reproductive performance in all indicators. Sows that were carriers of the mutant allele were characterized by lower values of the genetic productivity potential compared with the entire estimated population for all productive traits. Sows which were free of the mutant allele of the RYR1 gene were characterized by large values of the SIRQS index. The values of the coefficients of phenotypic consolidation of the number of live born piglets in sows without the mutant allele were lower than in sows with the mutant allele n. Better performance of sows free of the mutant allele of the RYR1 gene was established over sows carrying it in all evaluated traits of reproductive capacity (for different traits P ranged from 0.021 to 1.0*10–4), except for number of piglets born alive per sow in the daughter generation. Sows with the NN genotype had better selection index values by 15.7% in the maternal generation and by 10.2% in the daughter generation. In order to increase the reproductive ability of sows in the studied population of Welsh pigs and achieve similar results in other herds of this breed, animals free from the mutant allele of the RYR1 gene should be selected for further reproduction in the process of breeding, while on the contrary, carriers of this gene should be gradually eliminated from the herd. To carry out breeding work, further research is needed on the entire population of Welsh pigs for the RYR1 gene.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46441976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cadmium is a toxic heavy metal which is considered a dangerous environmental pollutant and has a detrimental effect on the organs of the reproductive system, the period of implantation and the development of embryos. The experiment presented in this article established the effect of cadmium salts (chloride and citrate) on the general progress of embryogenesis. For this purpose, 60 rats were randomly divided into three groups: control, experimental group with cadmium chloride exposure and experimental group with cadmium citrate exposure. Cadmium chloride solvent, cadmium citrate solvent at a dose of 1.0 mg/kg and distilled intragastric water were injected from the first to the thirteenth (first subgroup) and from the first to the twentieth days of embryogenesis (second subgroup). When cadmium chloride was injected, total embryonic (by 4.24 and 3.67 times), pre-implantation (by 6.50 and 14.03 times) and post-implantation mortality (by 3.07 and 2.49 times) increased with a reduction of the number of surviving fetuses by 24.0% and 25.9% compared with the control group on the 13th and 20th days of embryogenesis respectively. At the same time, during exposure to cadmium citrate, indicators of total embryonic mortality increased by 4.02 and 3.52 times, pre-implantation mortality by 6.04 and 13.03 times, and post-implantation mortality by 3.09 and 2.26 times, and indicators of the number of live fetuses decreased by 18.3% and 22.2% in relation to the control group. When determining the accumulation of cadmium in embryos on the 20th day of gestation, polyelement analysis of biological materials using the atomic emission method with electric arc atomization revealed a 15.83-fold increase in cadmium chloride and 9.00 times in cadmium citrate relative to the control group. Embryolethality rates increased in animals of both experimental groups while the number of live fetuses per female decreased, which indicated an obvious embryotoxic effect of cadmium compounds. It is would be useful to conduct histological studies, which will help detect changes at the tissue level and possibly explain the level of embryonic mortality.
{"title":"Toxicity of cadmium salts on indicators of embryogenesis of rats","authors":"I. Kolosova, V. Shatorna","doi":"10.15421/022243","DOIUrl":"https://doi.org/10.15421/022243","url":null,"abstract":"Cadmium is a toxic heavy metal which is considered a dangerous environmental pollutant and has a detrimental effect on the organs of the reproductive system, the period of implantation and the development of embryos. The experiment presented in this article established the effect of cadmium salts (chloride and citrate) on the general progress of embryogenesis. For this purpose, 60 rats were randomly divided into three groups: control, experimental group with cadmium chloride exposure and experimental group with cadmium citrate exposure. Cadmium chloride solvent, cadmium citrate solvent at a dose of 1.0 mg/kg and distilled intragastric water were injected from the first to the thirteenth (first subgroup) and from the first to the twentieth days of embryogenesis (second subgroup). When cadmium chloride was injected, total embryonic (by 4.24 and 3.67 times), pre-implantation (by 6.50 and 14.03 times) and post-implantation mortality (by 3.07 and 2.49 times) increased with a reduction of the number of surviving fetuses by 24.0% and 25.9% compared with the control group on the 13th and 20th days of embryogenesis respectively. At the same time, during exposure to cadmium citrate, indicators of total embryonic mortality increased by 4.02 and 3.52 times, pre-implantation mortality by 6.04 and 13.03 times, and post-implantation mortality by 3.09 and 2.26 times, and indicators of the number of live fetuses decreased by 18.3% and 22.2% in relation to the control group. When determining the accumulation of cadmium in embryos on the 20th day of gestation, polyelement analysis of biological materials using the atomic emission method with electric arc atomization revealed a 15.83-fold increase in cadmium chloride and 9.00 times in cadmium citrate relative to the control group. Embryolethality rates increased in animals of both experimental groups while the number of live fetuses per female decreased, which indicated an obvious embryotoxic effect of cadmium compounds. It is would be useful to conduct histological studies, which will help detect changes at the tissue level and possibly explain the level of embryonic mortality.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47940388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Zhuk, I. Sytnikova, T. Fylypchuk, O. Bahlei, O. Shkrobanets, J. Danihlík, H. Moskalyk, I. Panchuk, V. Burkut, P. Angelstam, M. Fedoriak
Physical and chemical quality indicators of 65 polyfloral honey samples from three administrative districts of Chernivtsi region in South Western Ukraine were studied. The chosen administrative districts were Putyla (representing the ‘Traditional villages’ stratum), Storozhynets (the ‘Intermediate’ stratum) and Khotyn (the ‘Intensive agriculture’ stratum), which reflect a steep gradient of social and ecological conditions such as land cover and land use, level of economic development, culture and demography. The quality of honey was determined in accordance with the requirements of the Ukrainian national standard and the EU Directive relating to honey (or Codex Alimentarius Honey Standard) by using the following indicators: reducing sugars and moisture content, diastase activity, free acidity, pH, electrical conductivity, hydroxymethylfurfural (HMF) and proline content. The profile of carbohydrates was analyzed, in particular glucose, fructose, sucrose, maltose, trehalose, melezitose, raffinose. The ratio of fructose to glucose (F/G) was determined. All tested samples complied with the international standards for the mass fraction of reducing sugars, diastase activity and hydroxymethylfurfural content. For physical and chemical parameters, the studied honey samples were of high quality. The content of the reducing sugars in the honey samples varied within the range of 66.0–97.6%. The fructose content ranged from 342 to 549 mg/g, and the glucose content variation ranged within 283–517 mg/g. The average fructose/glucose ratio was 1.2 for honey samples from the three studied districts. Besides fructose and glucose, some oligosaccharides, such as maltose, trehalose and melezitose, were found in the examined honey. Melezitose was detected in the honey samples from ‘Traditional village’ (21 samples) and ‘Intermediate’ (5 samples) districts. The total variability of HMF content in the studied 65 honey samples from apiaries in the three districts of Chernivtsi region ranged from 0.19 to 30.8 mg/kg. The minimum moisture content was found to be 16.2% (in the ‘Traditional village’ and ‘Intermediate’ strata), and 22.2% was the maximum (in the ‘Intermediate’ stratum). Our studies have shown that free acidity of the samples varied within the range 13.5 to 58.0 meq/kg. Proline content variability for the three studied geographical areas ranged from 82.3 to 1201.2 mg/kg. The studied samples of honey had a low pH level (~ 3.7), high content of proline (~ 513 mg/kg) and reducing sugars (~ 80%), which indicates its nutritional value and naturalness. Deviations from the honey standards in moisture content, acidity and electrical conductivity was revealed in 8% to 10% of all samples.
{"title":"Physicochemical quality indicators of honey: An evaluation in a Ukrainian socioecological gradient","authors":"A. Zhuk, I. Sytnikova, T. Fylypchuk, O. Bahlei, O. Shkrobanets, J. Danihlík, H. Moskalyk, I. Panchuk, V. Burkut, P. Angelstam, M. Fedoriak","doi":"10.15421/022246","DOIUrl":"https://doi.org/10.15421/022246","url":null,"abstract":"Physical and chemical quality indicators of 65 polyfloral honey samples from three administrative districts of Chernivtsi region in South Western Ukraine were studied. The chosen administrative districts were Putyla (representing the ‘Traditional villages’ stratum), Storozhynets (the ‘Intermediate’ stratum) and Khotyn (the ‘Intensive agriculture’ stratum), which reflect a steep gradient of social and ecological conditions such as land cover and land use, level of economic development, culture and demography. The quality of honey was determined in accordance with the requirements of the Ukrainian national standard and the EU Directive relating to honey (or Codex Alimentarius Honey Standard) by using the following indicators: reducing sugars and moisture content, diastase activity, free acidity, pH, electrical conductivity, hydroxymethylfurfural (HMF) and proline content. The profile of carbohydrates was analyzed, in particular glucose, fructose, sucrose, maltose, trehalose, melezitose, raffinose. The ratio of fructose to glucose (F/G) was determined. All tested samples complied with the international standards for the mass fraction of reducing sugars, diastase activity and hydroxymethylfurfural content. For physical and chemical parameters, the studied honey samples were of high quality. The content of the reducing sugars in the honey samples varied within the range of 66.0–97.6%. The fructose content ranged from 342 to 549 mg/g, and the glucose content variation ranged within 283–517 mg/g. The average fructose/glucose ratio was 1.2 for honey samples from the three studied districts. Besides fructose and glucose, some oligosaccharides, such as maltose, trehalose and melezitose, were found in the examined honey. Melezitose was detected in the honey samples from ‘Traditional village’ (21 samples) and ‘Intermediate’ (5 samples) districts. The total variability of HMF content in the studied 65 honey samples from apiaries in the three districts of Chernivtsi region ranged from 0.19 to 30.8 mg/kg. The minimum moisture content was found to be 16.2% (in the ‘Traditional village’ and ‘Intermediate’ strata), and 22.2% was the maximum (in the ‘Intermediate’ stratum). Our studies have shown that free acidity of the samples varied within the range 13.5 to 58.0 meq/kg. Proline content variability for the three studied geographical areas ranged from 82.3 to 1201.2 mg/kg. The studied samples of honey had a low pH level (~ 3.7), high content of proline (~ 513 mg/kg) and reducing sugars (~ 80%), which indicates its nutritional value and naturalness. Deviations from the honey standards in moisture content, acidity and electrical conductivity was revealed in 8% to 10% of all samples.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45694128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Stepanov, T. Tarasova, M. Stoikevych, Y. Gaydar, D. Mylostуva, О. Tatarchuk, O. Petishko
Taking into account the progress of understanding diagnosis, course prognosis, evaluation of effectiveness of therapy of inflammatory bowel disease and also differentiation diagnosis between its main forms – ulcerative colitis and Crohn’s disease, the search for efficient non-invasive markers for solving those issues is extremely relevant. The patients were divided into groups depending on nosology and severity of the course of the disease. All the patients had undergone endoscopic study for diagnosis verification and biopsy samples were taken for further detection of tissue IgG4 using the immunohistochemical method. Also, we determined concentration of serum IgG4. Increase in IgG4content in blood serum was determined in 54.0% of the cases of inflammatory bowel disease. Concentration of IgG4 in patients suffering ulcerative colitis was higher (by 2.31 and 2.46 times) compared with its level in the control group and patients with Crohn’s disease, respectively. We found relationships between the concentration of serum IgG4 and the activity of the disease. In patients with ulcerative colitis, increased tissue IgG4 was found more often than in patients with Crohn’s disease (by 2.77 times, Р < 0.05). We determined the relationship between tissue IgG4 and histological activity. Simultaneous increase in serum IgG4 and presence of tissue IgG4 during ulcerative colitis were more frequent than during Crohn’s disease (by 2.66 times). In all examined groups of patients, we determined decrease in serum IgG4 content (by 1.66 times) after treatment. Concentration of serum IgG4 and positive tissue IgG4 in ulcerative colitis patients exceeded such in Crohn’s disease patients, which may be used for differentiation diagnosis between those disease types. We determined dependence of IgG4 concentration on severity and duration of the disease, which could be used as a prognostic marker. Decrease in IgG4 content in blood serum against the background of the therapy shows that this indicator could be used as a marker of treatment efficacy. Perspectives of further studies are as follows: parameters of concentration of serum IgG4 and presence of tissue IgG4 could be used as diagnostic and prognostic biomarkers and be introduced to practice for differentiation diagnosis between ulcerative colitis and Crohn’s disease, and could be used as prognostic marker of severity of the disease and therapy efficacy.
{"title":"Diagnosis of inflammatory bowel disease according to human IgG4 and possibilities of evaluating efficacy of the therapy","authors":"Y. Stepanov, T. Tarasova, M. Stoikevych, Y. Gaydar, D. Mylostуva, О. Tatarchuk, O. Petishko","doi":"10.15421/022254","DOIUrl":"https://doi.org/10.15421/022254","url":null,"abstract":"Taking into account the progress of understanding diagnosis, course prognosis, evaluation of effectiveness of therapy of inflammatory bowel disease and also differentiation diagnosis between its main forms – ulcerative colitis and Crohn’s disease, the search for efficient non-invasive markers for solving those issues is extremely relevant. The patients were divided into groups depending on nosology and severity of the course of the disease. All the patients had undergone endoscopic study for diagnosis verification and biopsy samples were taken for further detection of tissue IgG4 using the immunohistochemical method. Also, we determined concentration of serum IgG4. Increase in IgG4content in blood serum was determined in 54.0% of the cases of inflammatory bowel disease. Concentration of IgG4 in patients suffering ulcerative colitis was higher (by 2.31 and 2.46 times) compared with its level in the control group and patients with Crohn’s disease, respectively. We found relationships between the concentration of serum IgG4 and the activity of the disease. In patients with ulcerative colitis, increased tissue IgG4 was found more often than in patients with Crohn’s disease (by 2.77 times, Р < 0.05). We determined the relationship between tissue IgG4 and histological activity. Simultaneous increase in serum IgG4 and presence of tissue IgG4 during ulcerative colitis were more frequent than during Crohn’s disease (by 2.66 times). In all examined groups of patients, we determined decrease in serum IgG4 content (by 1.66 times) after treatment. Concentration of serum IgG4 and positive tissue IgG4 in ulcerative colitis patients exceeded such in Crohn’s disease patients, which may be used for differentiation diagnosis between those disease types. We determined dependence of IgG4 concentration on severity and duration of the disease, which could be used as a prognostic marker. Decrease in IgG4 content in blood serum against the background of the therapy shows that this indicator could be used as a marker of treatment efficacy. Perspectives of further studies are as follows: parameters of concentration of serum IgG4 and presence of tissue IgG4 could be used as diagnostic and prognostic biomarkers and be introduced to practice for differentiation diagnosis between ulcerative colitis and Crohn’s disease, and could be used as prognostic marker of severity of the disease and therapy efficacy.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45800218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The analysis of cytogenetic activity is a key component in determining prospects of future hereditary variability after, subject to a certain mutagenic factor, primarily identifying the significance of the genotype-mutagenic interaction, the correctness of the selected concentrations for more thorough screening of some development parameters. Winter wheat seeds of eight varieties (Balaton, Borovytsia, Zeleny Gai, Zoloto Ukrainy, Kalancha, Niva Odeska, Polyanka, Pochayna) were subjected to ЕМS (ethylmethansulfonate) at the concentrations of 0.025%, 0.05%, 0.10% The exposure lasted for 24 hours. Cytogenetic analysis was carried out for pollen fertility; we also examined the rates and spectras of chromosomal rebuildings in proper cell devision phases in relation to plant gcnotype and concentration of the mutagen. The experiment was aimed at identification of interrection between geotype, concentration of mutagen and mutagen nature, determining genome response to mutagen action. Such indicators of cytogenetic activity as the total rate of chromosomal abnormalities, fragments and double fragments, single and double bridges, micronucleus and lagging chromosomes were studied. The selected concentrations of the mutagen significantly influenced all the analyzed parameters, they can be attributed to the optimal and high range of concentrations according to the nature of the impact on bread wheat. We determined that in the case of the mutagenic action, the genotype had a significantly lesser effect on the nature and rate of individual aberrations than an increase in the concentration, while having a significant effect on the rate of increase in pollen sterility. The mutagen was characterized by a significantly lower site-specificity at the cellular level than other chemical supermutagens, manifesting only in the correlation between individual types of aberrations, but not in the character of the increase in their number. The key parameter to identify the activity of this agent was the frequency of fragments and double fragments, their ratio with bridges.
{"title":"Winter wheat cytogenetic variability under the action of a chemical supermutagen","authors":"V. Horshchar, M. Nazarenko","doi":"10.15421/022249","DOIUrl":"https://doi.org/10.15421/022249","url":null,"abstract":"The analysis of cytogenetic activity is a key component in determining prospects of future hereditary variability after, subject to a certain mutagenic factor, primarily identifying the significance of the genotype-mutagenic interaction, the correctness of the selected concentrations for more thorough screening of some development parameters. Winter wheat seeds of eight varieties (Balaton, Borovytsia, Zeleny Gai, Zoloto Ukrainy, Kalancha, Niva Odeska, Polyanka, Pochayna) were subjected to ЕМS (ethylmethansulfonate) at the concentrations of 0.025%, 0.05%, 0.10% The exposure lasted for 24 hours. Cytogenetic analysis was carried out for pollen fertility; we also examined the rates and spectras of chromosomal rebuildings in proper cell devision phases in relation to plant gcnotype and concentration of the mutagen. The experiment was aimed at identification of interrection between geotype, concentration of mutagen and mutagen nature, determining genome response to mutagen action. Such indicators of cytogenetic activity as the total rate of chromosomal abnormalities, fragments and double fragments, single and double bridges, micronucleus and lagging chromosomes were studied. The selected concentrations of the mutagen significantly influenced all the analyzed parameters, they can be attributed to the optimal and high range of concentrations according to the nature of the impact on bread wheat. We determined that in the case of the mutagenic action, the genotype had a significantly lesser effect on the nature and rate of individual aberrations than an increase in the concentration, while having a significant effect on the rate of increase in pollen sterility. The mutagen was characterized by a significantly lower site-specificity at the cellular level than other chemical supermutagens, manifesting only in the correlation between individual types of aberrations, but not in the character of the increase in their number. The key parameter to identify the activity of this agent was the frequency of fragments and double fragments, their ratio with bridges.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41856293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Dubrovna, G. Priadkina, S. Mykhalska, A. Komisarenko
The global climate changes and the consequent increase in the number of soil and air droughts during the vegetation period of grain crops require the development of new strategies to adapt plants to those yield-decreasing stressors. A relevant way of increasing drought-tolerance of cereals is the use of biotechnological methods, particularly RNA interference, which can down-regulate the activity of plants’ genes and increase concentration of stress metabolites that perform osmoprotective functions during drought. We studied the tolerance to soil moisture shortage in transgenic plants of winter wheat with partial suppression of the proline dehydrogenase gene, obtained using the technology of short interfering RNAs. We analyzed physiological and biochemical parameters and structural elements of yield productivity of 4 wild genotypes and their transgenic lines with reduced activity of proline dehydrogenase in the conditions of 7-day drought during the late booting–ear emergence. We determined that the presence of double-stranded RNA suppressor of the proline dehydrogenase gene in transgenic lines led to increase in the level of accumulation of free proline in flag leaves. At the same time, its concentration in transgenic lines was higher than in untransformed plants of the wild genotypes in both drought conditions and conditions of sufficient moisture. We found that against the background of water deficiency, the total chlorophyll content in leaves of plants of transgenic lines was significantly higher, and the ratio of carotenoids to chlorophyll was lower than in plants of the wild genotypes, suggesting mitigation of the negative impact of drought on the plants of transgenic lines. Lacking soil moisture, genetically altered lines of wheat had significantly higher parameters of the structure of grain yield compared with untransformed genotypes. At the same time, we observed genotypic difference according to grain productivity in biotechnological plants. Therefore, the results we obtained confirm the perspectives of using the technology of short interfering RNAs to increase tolerance of winter wheat to water deficiency.
{"title":"Drought-tolerance of transgenic winter wheat with partial suppression of the proline dehydrogenase gene","authors":"O. Dubrovna, G. Priadkina, S. Mykhalska, A. Komisarenko","doi":"10.15421/022251","DOIUrl":"https://doi.org/10.15421/022251","url":null,"abstract":"The global climate changes and the consequent increase in the number of soil and air droughts during the vegetation period of grain crops require the development of new strategies to adapt plants to those yield-decreasing stressors. A relevant way of increasing drought-tolerance of cereals is the use of biotechnological methods, particularly RNA interference, which can down-regulate the activity of plants’ genes and increase concentration of stress metabolites that perform osmoprotective functions during drought. We studied the tolerance to soil moisture shortage in transgenic plants of winter wheat with partial suppression of the proline dehydrogenase gene, obtained using the technology of short interfering RNAs. We analyzed physiological and biochemical parameters and structural elements of yield productivity of 4 wild genotypes and their transgenic lines with reduced activity of proline dehydrogenase in the conditions of 7-day drought during the late booting–ear emergence. We determined that the presence of double-stranded RNA suppressor of the proline dehydrogenase gene in transgenic lines led to increase in the level of accumulation of free proline in flag leaves. At the same time, its concentration in transgenic lines was higher than in untransformed plants of the wild genotypes in both drought conditions and conditions of sufficient moisture. We found that against the background of water deficiency, the total chlorophyll content in leaves of plants of transgenic lines was significantly higher, and the ratio of carotenoids to chlorophyll was lower than in plants of the wild genotypes, suggesting mitigation of the negative impact of drought on the plants of transgenic lines. Lacking soil moisture, genetically altered lines of wheat had significantly higher parameters of the structure of grain yield compared with untransformed genotypes. At the same time, we observed genotypic difference according to grain productivity in biotechnological plants. Therefore, the results we obtained confirm the perspectives of using the technology of short interfering RNAs to increase tolerance of winter wheat to water deficiency.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47424362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Chechet, V. Kovalenko, O. I. Horbatyuk, O. S. Gaidei, O. Kravtsova, V. Andriyashchuk, I. Musiets, D. Ordynska
Complex probiotic preparations with spore-forming microorganisms of the genus Bacillus and other types of bacteria in their composition have a high biological activity and are of scientific and practical interest. In our laboratory experiments, we determined the level of antagonistic activity of the newly developed probiotic preparation containing strains of bacteria B. subtilis, B. licheniformis, B. coagulans in a complex with Enterococcus faecium, products of their own fermentation, other natural substances and a sorbent made of natural aluminosilicates. The antagonistic properties of the probiotic preparation were examined by diffusion methods of delayed antagonism and agar blocks. In order to identify the antagonistic action of the experimental probiotic preparation, we used Gram-negative and Gram-positive test cultures of bacteria from the Museum of Strains of Microorganism Cultures of the Research Bacteriology Department. The results of laboratory studies indicated the antagonistic effectiveness of the experimental probiotic preparation in its interaction with test microorganisms. This was confirmed by the indicators of the diameters of the growth inhibition zones of the tested bacteria, ranging 27–36 and more and corresponding to high and very high levels of antagonistic activity of the experimental probiotic. The use of the developed probiotic preparation in poultry farming would ensure the correct formation of microbiocenosis in gthe astrointestinal tract of poultry from hatching, increase local immunity, support a stable epizootic situation by preventing bacterial infections among poultry through biological means, and therefore contribute to production of ecologically high-quality and safe products of poultry farming.
{"title":"Antagonistic properties of a probiotic preparation containing bacteria of the genera Bacillus and Enterococcus","authors":"O. Chechet, V. Kovalenko, O. I. Horbatyuk, O. S. Gaidei, O. Kravtsova, V. Andriyashchuk, I. Musiets, D. Ordynska","doi":"10.15421/022247","DOIUrl":"https://doi.org/10.15421/022247","url":null,"abstract":"Complex probiotic preparations with spore-forming microorganisms of the genus Bacillus and other types of bacteria in their composition have a high biological activity and are of scientific and practical interest. In our laboratory experiments, we determined the level of antagonistic activity of the newly developed probiotic preparation containing strains of bacteria B. subtilis, B. licheniformis, B. coagulans in a complex with Enterococcus faecium, products of their own fermentation, other natural substances and a sorbent made of natural aluminosilicates. The antagonistic properties of the probiotic preparation were examined by diffusion methods of delayed antagonism and agar blocks. In order to identify the antagonistic action of the experimental probiotic preparation, we used Gram-negative and Gram-positive test cultures of bacteria from the Museum of Strains of Microorganism Cultures of the Research Bacteriology Department. The results of laboratory studies indicated the antagonistic effectiveness of the experimental probiotic preparation in its interaction with test microorganisms. This was confirmed by the indicators of the diameters of the growth inhibition zones of the tested bacteria, ranging 27–36 and more and corresponding to high and very high levels of antagonistic activity of the experimental probiotic. The use of the developed probiotic preparation in poultry farming would ensure the correct formation of microbiocenosis in gthe astrointestinal tract of poultry from hatching, increase local immunity, support a stable epizootic situation by preventing bacterial infections among poultry through biological means, and therefore contribute to production of ecologically high-quality and safe products of poultry farming.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42706943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Korniienko, V. Ukhovskyi, O. A. Moroz, O. Chechet, O. Haidei, T. Tsarenko, T. M. Bondarenko, M. Karpulenko, N. P. Nenych
The problem of zoonoses remains relevant in the context of reliable prevention of human disease and effective ways to achieve this result, in particular through the impact on susceptible animals by the efforts of veterinary medicine. Anthrax is an acute, particularly dangerous infectious disease of all species of farm, domestic and wild animals, as well as humans, which is caused by Bacillus anthracis microbes. The causative agent of anthrax belongs to the group of aerobic spore-forming bacteria and exists in two main forms: vegetative and spore. The vegetative form in the body of an infected animal can form a "capsule". In Ukraine, according to the provisions of the current "Instruction for the prevention and control of animal anthrax" (2000), the main method of preventing anthrax among animals is regular vaccination of animals susceptible to this disease. The authors conducted a retrospective analysis of the epizootic and epidemiological situation of anthrax in Ukraine for the period 1994–2021 and made a critical assessment of the performance of mandatory measures for specific prevention of susceptible animals. In order to find out the ecological and geographical features of the spread of anthrax, data on outbreaks of the disease in cattle, swine, small ruminants and humans on the territory of Ukraine were analyzed by regions for the time period under investigation. Over the past 28 years in Ukraine, animal anthrax was registered in in all areas except Zhytomyr region. In total, during the analyzed period, 177 affected points and 637 infected animals (cattle, small ruminants, pigs, horses, wild and fur-bearing animals, dogs) were registered (estimated at 3.59 animals per outbreak). Cattle were most often involved in the epizootic process, followed by pigs and small ruminants, while horses and other animal species were least infected. Ecological and geographical analysis showed that the largest number of affected points among animals during the analyzed period was found in Kyiv, Volyn, Kharkiv, Luhansk, Khmelnytskyi, Cherkasy, Odesa, and Vinnytsia regions. A small number of affected points during the analyzed period were found in Zakarpattia, Ternopil, Kherson, Autonomous Republic of Crimea, Poltava, Dnipropetrovsk and Ivano-Frankivsk regions. During the analyzed period, 68 people in 11 regions of Ukraine were infected with anthrax, 15 outbreaks were registered (4.46 people per outbreak). Most cases were reported in Donetsk, Kyiv and Odesa regions. The association between outbreaks of anthrax in animals and cases of anthrax among humans has been established, this dependency was 86.6% (the index of contiguity, which takes into account the number of years with simultaneous registration of animal and human cases, was 0.5). The authors thoroughly proved that it is vaccination among susceptible animals that will finally prevent the incidence of anthrax among people.
{"title":"Epizootological and epidemiological situation of anthrax in Ukraine in the context of mandatory specific prevention in susceptible animals","authors":"L. Korniienko, V. Ukhovskyi, O. A. Moroz, O. Chechet, O. Haidei, T. Tsarenko, T. M. Bondarenko, M. Karpulenko, N. P. Nenych","doi":"10.15421/022245","DOIUrl":"https://doi.org/10.15421/022245","url":null,"abstract":"The problem of zoonoses remains relevant in the context of reliable prevention of human disease and effective ways to achieve this result, in particular through the impact on susceptible animals by the efforts of veterinary medicine. Anthrax is an acute, particularly dangerous infectious disease of all species of farm, domestic and wild animals, as well as humans, which is caused by Bacillus anthracis microbes. The causative agent of anthrax belongs to the group of aerobic spore-forming bacteria and exists in two main forms: vegetative and spore. The vegetative form in the body of an infected animal can form a \"capsule\". In Ukraine, according to the provisions of the current \"Instruction for the prevention and control of animal anthrax\" (2000), the main method of preventing anthrax among animals is regular vaccination of animals susceptible to this disease. The authors conducted a retrospective analysis of the epizootic and epidemiological situation of anthrax in Ukraine for the period 1994–2021 and made a critical assessment of the performance of mandatory measures for specific prevention of susceptible animals. In order to find out the ecological and geographical features of the spread of anthrax, data on outbreaks of the disease in cattle, swine, small ruminants and humans on the territory of Ukraine were analyzed by regions for the time period under investigation. Over the past 28 years in Ukraine, animal anthrax was registered in in all areas except Zhytomyr region. In total, during the analyzed period, 177 affected points and 637 infected animals (cattle, small ruminants, pigs, horses, wild and fur-bearing animals, dogs) were registered (estimated at 3.59 animals per outbreak). Cattle were most often involved in the epizootic process, followed by pigs and small ruminants, while horses and other animal species were least infected. Ecological and geographical analysis showed that the largest number of affected points among animals during the analyzed period was found in Kyiv, Volyn, Kharkiv, Luhansk, Khmelnytskyi, Cherkasy, Odesa, and Vinnytsia regions. A small number of affected points during the analyzed period were found in Zakarpattia, Ternopil, Kherson, Autonomous Republic of Crimea, Poltava, Dnipropetrovsk and Ivano-Frankivsk regions. During the analyzed period, 68 people in 11 regions of Ukraine were infected with anthrax, 15 outbreaks were registered (4.46 people per outbreak). Most cases were reported in Donetsk, Kyiv and Odesa regions. The association between outbreaks of anthrax in animals and cases of anthrax among humans has been established, this dependency was 86.6% (the index of contiguity, which takes into account the number of years with simultaneous registration of animal and human cases, was 0.5). The authors thoroughly proved that it is vaccination among susceptible animals that will finally prevent the incidence of anthrax among people.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41846413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The coronavirus disease 2019 (COVID-19) affects humans, who are now developing a milder form of the illness. In addition to the use of available drugs, the local populations in Morocco used medicinal plants during the pandemic. The current study aimed to document and to research the level of use of traditional medicine using plants in Morocco. Ethnobotanical surveys were conducted on the perceptions and level of use of plants through a case study in North-West Morocco. The methodology is based on targeted surveys, from April to September 2021, of households that have had cases if nfection with COVID-19. Five hundred surveys were randomly and systematically distributed in the study area to highlight the medicinal use of plants to treat COVID-19 and its consequences for health and well-being. The results showed the attachment of the populations to traditional medicine with the use of plants available in the study area (local forest area and herbalists). The 23 species used by the populations in question against COVD-19 belong to 12 families. The analysis shows the dominance of the Lamiaceae family with nine species, followed by Asteraceae with three species. The Lamiaceae family have proven marked performance in the fight against COVID-19. The results obtained offer orientations for the enhancement of the beneficial effects of traditional medicine and clinical trials remain to be carried out. The current results highlight that flora can be a source for the discovery of medicinal agents active against COVID-19.
{"title":"Contribution to the identification of medicinal plants used against COVID-19 in North-West Morocco","authors":"S. Laaribya, A. Alaoui, R. Azmi","doi":"10.15421/022244","DOIUrl":"https://doi.org/10.15421/022244","url":null,"abstract":"The coronavirus disease 2019 (COVID-19) affects humans, who are now developing a milder form of the illness. In addition to the use of available drugs, the local populations in Morocco used medicinal plants during the pandemic. The current study aimed to document and to research the level of use of traditional medicine using plants in Morocco. Ethnobotanical surveys were conducted on the perceptions and level of use of plants through a case study in North-West Morocco. The methodology is based on targeted surveys, from April to September 2021, of households that have had cases if nfection with COVID-19. Five hundred surveys were randomly and systematically distributed in the study area to highlight the medicinal use of plants to treat COVID-19 and its consequences for health and well-being. The results showed the attachment of the populations to traditional medicine with the use of plants available in the study area (local forest area and herbalists). The 23 species used by the populations in question against COVD-19 belong to 12 families. The analysis shows the dominance of the Lamiaceae family with nine species, followed by Asteraceae with three species. The Lamiaceae family have proven marked performance in the fight against COVID-19. The results obtained offer orientations for the enhancement of the beneficial effects of traditional medicine and clinical trials remain to be carried out. The current results highlight that flora can be a source for the discovery of medicinal agents active against COVID-19.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44380026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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