Pub Date : 2018-09-30DOI: 10.21608/RPBS.2018.3874.1004
Safaa M Kishk, M. Helal, M. Gomaa, I. Salama, S. Moustafa, C. Simons
Tuberculosis (TB) is the ninth leading cause of death worldwide and the leading cause from a single infectious agent, ranking above HIV/AIDS with 6.3 million new cases of TB reported in 2016. TB is an air-borne disease associated with the aerobic bacterium Mycobacterium tuberculosis (Mtb), which mainly infects the lungs. Aerosolization of diseased pulmonary secretions, by coughing, sneezing and speaking, discharge the Mtb bacilli into the atmosphere. Infected aerosol droplet nuclei sized 1-10 μm are largely trapped in the upper nasal passages or are expelled into the pharynx by the mucociliary mechanism of the lower respiratory tract and are harmlessly swallowed and digested. Infected persons may overcome the initial TB infection, resulting in the development of asymptomatic latent TB. About 10% of individuals may develop the active disease after infection; where the bacteria undergo more rapid growth and overcome the host immune system. In cases of multi-drug resistant (MDR) strains, and extreme drug-resistant (XDR) strains, treatment fails, and the bacteria propagate and attack the host, leading to death from systemic infection. Due to the increased spread of TB worldwide, both the academic and industrial communities have initiated intensive research to develop new therapeutics targeting new enzymes such as cytochrome P450s in Mtb.
{"title":"Insights into Novel Drug Targets in Mycobacterium tuberculosis: Where Do We Stand and Where Do We Go from Here?","authors":"Safaa M Kishk, M. Helal, M. Gomaa, I. Salama, S. Moustafa, C. Simons","doi":"10.21608/RPBS.2018.3874.1004","DOIUrl":"https://doi.org/10.21608/RPBS.2018.3874.1004","url":null,"abstract":"Tuberculosis (TB) is the ninth leading cause of death worldwide and the leading cause from a single infectious agent, ranking above HIV/AIDS with 6.3 million new cases of TB reported in 2016. TB is an air-borne disease associated with the aerobic bacterium Mycobacterium tuberculosis (Mtb), which mainly infects the lungs. Aerosolization of diseased pulmonary secretions, by coughing, sneezing and speaking, discharge the Mtb bacilli into the atmosphere. Infected aerosol droplet nuclei sized 1-10 μm are largely trapped in the upper nasal passages or are expelled into the pharynx by the mucociliary mechanism of the lower respiratory tract and are harmlessly swallowed and digested. Infected persons may overcome the initial TB infection, resulting in the development of asymptomatic latent TB. About 10% of individuals may develop the active disease after infection; where the bacteria undergo more rapid growth and overcome the host immune system. In cases of multi-drug resistant (MDR) strains, and extreme drug-resistant (XDR) strains, treatment fails, and the bacteria propagate and attack the host, leading to death from systemic infection. Due to the increased spread of TB worldwide, both the academic and industrial communities have initiated intensive research to develop new therapeutics targeting new enzymes such as cytochrome P450s in Mtb.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87633343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-30DOI: 10.21608/RPBS.2018.3642.1001
Naglaa K gawada
Skeletal muscle atrophy is one of the serious and less studied diabetic complications.Leucine an essential amino acid that is transported into most mammalian cells by System L. The current study was conducted to assess the effect of leucine on diabetic skeletal muscles. Forty male Wister rats were allocated into four groups; control, Leucine-treated group was subjected to daily oral supplement of Leucine (1.35 g/kg) for 8 weeks, STZ-diabetic group was treated with single intravenous injection of STZ (45 mg/kg) and STZ-diabetic group supplemented with leucine (1.35 g/kg) for 8 weeks. Body weight and histopathological analysis of soleus muscle were evaluated. There was a significant increase in body weight of leucine group at p> 0.05. Leucine supplementation attenuated loss of soleus muscle mass observed in STZ diabetic rats. These findings may suggest protective effect of leucine against diabetic muscle loss.
{"title":"The effect of leucine supplementation on the skeletal muscles of streptozotocin (STZ)diabetic rats","authors":"Naglaa K gawada","doi":"10.21608/RPBS.2018.3642.1001","DOIUrl":"https://doi.org/10.21608/RPBS.2018.3642.1001","url":null,"abstract":"Skeletal muscle atrophy is one of the serious and less studied diabetic complications.Leucine an essential amino acid that is transported into most mammalian cells by System L. The current study was conducted to assess the effect of leucine on diabetic skeletal muscles. Forty male Wister rats were allocated into four groups; control, Leucine-treated group was subjected to daily oral supplement of Leucine (1.35 g/kg) for 8 weeks, STZ-diabetic group was treated with single intravenous injection of STZ (45 mg/kg) and STZ-diabetic group supplemented with leucine (1.35 g/kg) for 8 weeks. Body weight and histopathological analysis of soleus muscle were evaluated. There was a significant increase in body weight of leucine group at p> 0.05. Leucine supplementation attenuated loss of soleus muscle mass observed in STZ diabetic rats. These findings may suggest protective effect of leucine against diabetic muscle loss.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87669474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-30DOI: 10.21608/RPBS.2018.3769.1002
E. Shawky, H. Hammoda, Amina Abou Donia, S. Toaima, Erii Kinoshita, H. Takayama
A phytochemical investigation of the alkaloidal content of the bulbs of Narcissus pseudonarcissus cultivated in Egypt resulted in the isolation of the alkaloid; 9-O-demethyl-7-O-methyllycorenine (5) in addition to the eight known alkaloids; homolycorine (1), 7-O-methyloduline (2), 7-O-methyllycorenine (3), hippeastrine (4), 9-O-demethylhomolycorine (6), galanthamine (7), haemanthamine (8) and lycorine. Structural determination of the isolated alkaloids was established by different spectral analyses (UV, MS, NMR and 2D-NMR). The isolation of 9-O-demethyl-7-O-methyllycorenine (5) is reported here for the first time from family Amaryllidaceae. The chemotaxonomic significance of the isolated alkaloids was also studied; six of the isolated alkaloids belong to the homolycorine series which is a distinctive feature for the section Pseudonarcissi. The alkaloids of the homolycorine series are absent from some tribes of the Amaryllidaceae, such as the Amaryllideae or Hemantheae. Moreover, all the Narcissus alkaloids of the homolycorine series display a B/C ring junction with a cis stereochemistry Furthermore, the antimicrobial activity of some isolated alkaloids has been studied. It is noteworthy that this is the first phytochemical and biological investigation to be carried out on the Amaryllidaceae plant N. pseudonarcissus grown in Egypt.
在埃及栽培的水仙(Narcissus pseudonarcissus)球茎中进行了生物碱含量的植物化学研究，分离出了其中的生物碱;9- o -去甲基-7- o -甲基番茄碱(5)以及八种已知生物碱;同质多碱(1)、7- o -甲基多碱(2)、7- o -甲基蕃茄碱(3)、海马碱(4)、9- o -去甲基同质多碱(6)、加兰他敏(7)、海毒胺(8)和石蒜碱。采用紫外、质谱、核磁共振和二维核磁共振等不同光谱分析方法对分离得到的生物碱进行了结构鉴定。9-O-demethyl-7-O-methyllycorenine(5)是首次从Amaryllidaceae中分离得到。对分离得到的生物碱的化学分类意义进行了研究;6个分离的生物碱属于同质多碱系列，这是Pseudonarcissi剖面的一个显著特征。同绿碱系列的生物碱在菊科的一些部落，如菊科或Hemantheae中不存在。水仙碱系列生物碱均呈B/C环结，具有顺式立体化学结构。此外，对部分分离生物碱的抑菌活性进行了研究。值得注意的是，这是首次对生长在埃及的amaryllidiaceae植物N. pseudonarcissus进行植物化学和生物学研究。
{"title":"Phytochemical and Biological Investigation of Narcissus pseudonarcissus Cultivated in Egypt","authors":"E. Shawky, H. Hammoda, Amina Abou Donia, S. Toaima, Erii Kinoshita, H. Takayama","doi":"10.21608/RPBS.2018.3769.1002","DOIUrl":"https://doi.org/10.21608/RPBS.2018.3769.1002","url":null,"abstract":"A phytochemical investigation of the alkaloidal content of the bulbs of Narcissus pseudonarcissus cultivated in Egypt resulted in the isolation of the alkaloid; 9-O-demethyl-7-O-methyllycorenine (5) in addition to the eight known alkaloids; homolycorine (1), 7-O-methyloduline (2), 7-O-methyllycorenine (3), hippeastrine (4), 9-O-demethylhomolycorine (6), galanthamine (7), haemanthamine (8) and lycorine. Structural determination of the isolated alkaloids was established by different spectral analyses (UV, MS, NMR and 2D-NMR). The isolation of 9-O-demethyl-7-O-methyllycorenine (5) is reported here for the first time from family Amaryllidaceae. The chemotaxonomic significance of the isolated alkaloids was also studied; six of the isolated alkaloids belong to the homolycorine series which is a distinctive feature for the section Pseudonarcissi. The alkaloids of the homolycorine series are absent from some tribes of the Amaryllidaceae, such as the Amaryllideae or Hemantheae. Moreover, all the Narcissus alkaloids of the homolycorine series display a B/C ring junction with a cis stereochemistry Furthermore, the antimicrobial activity of some isolated alkaloids has been studied. It is noteworthy that this is the first phytochemical and biological investigation to be carried out on the Amaryllidaceae plant N. pseudonarcissus grown in Egypt.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89240807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-30DOI: 10.21608/RPBS.2018.4273.1006
Rasha M. Ahmed, Ghada M. Hadad, A. El-Gendy, A. Ashour
A Quality by Design approach was used to develop a sensitive spectrophotometric method for determination of glucosamine Sulphate in pharmaceutical dosage forms and optimize a derivatization reaction of glucosamine with o-phthalaldhyde. This strategy was focused on derivatization process understanding and analytical method development and validation. Both of them were evaluated by applying risk assessment and Design Space tools. The scouting step was carried out by analyzing different derivatizing agents, type of buffers, pH of buffers, and time of reaction. Five critical process parameters (CPPs) were selected related to, pH of borate buffer, volume of 2-mercaptoethanol (ME), volume of o-phthalaldhyde (OPA), and the time of reaction. The effect of different levels of CPPs on critical quality attributes (CQAs) (the absorbance of derivative) was evaluated in the screening design using fractional factorial design. Using response surface methodology and contour plots the optimum values of the selected factors were determined and the design space of the method was defined. Finally, control strategy was designed and the method was validated and applied to a real sample of glucosamine dosage forms using the optimum derivatization conditions of the reaction; pH of borate buffer 10.5, concentration of buffer 0.05M, Time of reaction 10.7 minutes, volume of ME 100μL, and volume of OPA 5.1mL at room temperature. The results of the present study clearly shown that the developed method was sensitive relative to the other published spectrophotometric and HPLC methods with identification of the most significant factors affecting the derivatization reaction.
{"title":"Spectrophotometric method for determination of Glucosamine Sulphate in dosage forms by derivatization using Quality by Design approach","authors":"Rasha M. Ahmed, Ghada M. Hadad, A. El-Gendy, A. Ashour","doi":"10.21608/RPBS.2018.4273.1006","DOIUrl":"https://doi.org/10.21608/RPBS.2018.4273.1006","url":null,"abstract":"A Quality by Design approach was used to develop a sensitive spectrophotometric method for determination of glucosamine Sulphate in pharmaceutical dosage forms and optimize a derivatization reaction of glucosamine with o-phthalaldhyde. This strategy was focused on derivatization process understanding and analytical method development and validation. Both of them were evaluated by applying risk assessment and Design Space tools. The scouting step was carried out by analyzing different derivatizing agents, type of buffers, pH of buffers, and time of reaction. Five critical process parameters (CPPs) were selected related to, pH of borate buffer, volume of 2-mercaptoethanol (ME), volume of o-phthalaldhyde (OPA), and the time of reaction. The effect of different levels of CPPs on critical quality attributes (CQAs) (the absorbance of derivative) was evaluated in the screening design using fractional factorial design. Using response surface methodology and contour plots the optimum values of the selected factors were determined and the design space of the method was defined. Finally, control strategy was designed and the method was validated and applied to a real sample of glucosamine dosage forms using the optimum derivatization conditions of the reaction; pH of borate buffer 10.5, concentration of buffer 0.05M, Time of reaction 10.7 minutes, volume of ME 100μL, and volume of OPA 5.1mL at room temperature. The results of the present study clearly shown that the developed method was sensitive relative to the other published spectrophotometric and HPLC methods with identification of the most significant factors affecting the derivatization reaction.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81499147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-01DOI: 10.21608/RPBS.2018.5337.1013
hanim ameer, D. Abo-Elmatty, R. Alemany, N. Mesbah
The use of Anabolic Androgenic Steroid agents is evident within the competitive sport in senior and junior age groups, where they are taken by non-elite as well as elite participants. They are also taken in non-sporting contexts by individuals seeking to ‘improve’ their physique through an increase in muscle and/or decrease in fat mass. No accurate data is available for the prevalence of anabolic steroid misuse among athletes, studies suggest the illicit use of doping agents by athletes and non-athletes may be 1–5% in the population and greater than 50% in some groups; with the prevalence being higher in males. Many studies documented side effects and health hazards with the misusing of anabolic steroids, where these were accused as a cause of deaths among athletes. Intake of exogenous anabolic steroids disturbed the T/E ratio causing its evaluation above the normal level. This review will outline the anabolic steroids, its side effects and health impacts in both the sporting and physique development contexts. It also provides a brief review of the history of AAS as doping agents and athlete biological passport.
{"title":"Effect of Exogenous Anabolic Androgenic Steroids on Testosterone Epitestosterone Ratio and its Application on Athlete Biological Passport in Egypt","authors":"hanim ameer, D. Abo-Elmatty, R. Alemany, N. Mesbah","doi":"10.21608/RPBS.2018.5337.1013","DOIUrl":"https://doi.org/10.21608/RPBS.2018.5337.1013","url":null,"abstract":"The use of Anabolic Androgenic Steroid agents is evident within the competitive sport in senior and junior age groups, where they are taken by non-elite as well as elite participants. They are also taken in non-sporting contexts by individuals seeking to ‘improve’ their physique through an increase in muscle and/or decrease in fat mass. No accurate data is available for the prevalence of anabolic steroid misuse among athletes, studies suggest the illicit use of doping agents by athletes and non-athletes may be 1–5% in the population and greater than 50% in some groups; with the prevalence being higher in males. Many studies documented side effects and health hazards with the misusing of anabolic steroids, where these were accused as a cause of deaths among athletes. Intake of exogenous anabolic steroids disturbed the T/E ratio causing its evaluation above the normal level. This review will outline the anabolic steroids, its side effects and health impacts in both the sporting and physique development contexts. It also provides a brief review of the history of AAS as doping agents and athlete biological passport.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"50 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91476781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-01DOI: 10.21608/RPBS.2018.6184.1017
K. Afify, Randa A. Abdel-Salam, Ghada M. Hadad
A new HPLC method for the determination of Metronidazole was established. The determination was performed by using a Kinetex C18 analytical column with a gradient mobile phase system consisting of 0.05M KH₂PO4 (pH 3.5), pH adjusted with ortho phosphoric acid (mobile phase A) and Acetonitrile (mobile phase B). The flow rate was 1ml/min. and quantitation was achieved with UV detection at 230 nm, based on peak area. The retention time obtained was 5.35 ± 0.004 and the method was validated according to ICH guidelines regarding linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, robustness, and selectivity. Excellent linearity was observed for the calibration curve with an excellent correlation coefficient 0.9999. Limit of detection was 0.78 μg mL−1; limit of quantitation 2.37 μg mL−1. The developed method was found to be accurate and sensitive and is ideally suited for analysis of MTZ in pure form, pharmaceutical dosage form and spiked urine samples.
{"title":"HPLC determination of Metronidazole in pure form, pharmaceutical dosage form and spiked urine.","authors":"K. Afify, Randa A. Abdel-Salam, Ghada M. Hadad","doi":"10.21608/RPBS.2018.6184.1017","DOIUrl":"https://doi.org/10.21608/RPBS.2018.6184.1017","url":null,"abstract":"A new HPLC method for the determination of Metronidazole was established. The determination was performed by using a Kinetex C18 analytical column with a gradient mobile phase system consisting of 0.05M KH₂PO4 (pH 3.5), pH adjusted with ortho phosphoric acid (mobile phase A) and Acetonitrile (mobile phase B). The flow rate was 1ml/min. and quantitation was achieved with UV detection at 230 nm, based on peak area. The retention time obtained was 5.35 ± 0.004 and the method was validated according to ICH guidelines regarding linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, robustness, and selectivity. Excellent linearity was observed for the calibration curve with an excellent correlation coefficient 0.9999. Limit of detection was 0.78 μg mL−1; limit of quantitation 2.37 μg mL−1. The developed method was found to be accurate and sensitive and is ideally suited for analysis of MTZ in pure form, pharmaceutical dosage form and spiked urine samples.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85552055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-01DOI: 10.21608/RPBS.2018.5986.1016
Heba K. Badawy, Noha Mesbah, D. Abo-Elmatty
Essential hypertension is a chronic medical condition affecting thousands of people worldwide. It is polygenic and multi-factorial disease resulting from the interaction between genetic and environmental factors. Essential hypertension is implicated in cerebrovascular, cardiovascular and renal diseases. MicroRNAs are considered endogenous, non-coding regulators of gene expression by targeting specific mRNAs for degradation and/ or translational repression. MicroRNAs participate in a variety of developmental processes as metabolism, cell proliferation, and apoptosis. Many studies have reported the possibility of using miRNAs as new biological markers for polygenic diseases as cancer, stem cell aging, coronary heart disease, and essential hypertension. The stability of these miRNAs as miRNA let-7e, miRNA 296-5p, miRNA 605, and miRNA 623 in biological fluids makes them amenable to detection and quantification. This review summarizes the mechanisms by which miRNAs can control normal cellular processes and gene expression and the association of some circulating miRNAs with the incidence of different diseases, particularly essential hypertension.
{"title":"A Brief review of Circulating MicroRNAs and Essential Hypertension","authors":"Heba K. Badawy, Noha Mesbah, D. Abo-Elmatty","doi":"10.21608/RPBS.2018.5986.1016","DOIUrl":"https://doi.org/10.21608/RPBS.2018.5986.1016","url":null,"abstract":"Essential hypertension is a chronic medical condition affecting thousands of people worldwide. It is polygenic and multi-factorial disease resulting from the interaction between genetic and environmental factors. Essential hypertension is implicated in cerebrovascular, cardiovascular and renal diseases. MicroRNAs are considered endogenous, non-coding regulators of gene expression by targeting specific mRNAs for degradation and/ or translational repression. MicroRNAs participate in a variety of developmental processes as metabolism, cell proliferation, and apoptosis. Many studies have reported the possibility of using miRNAs as new biological markers for polygenic diseases as cancer, stem cell aging, coronary heart disease, and essential hypertension. The stability of these miRNAs as miRNA let-7e, miRNA 296-5p, miRNA 605, and miRNA 623 in biological fluids makes them amenable to detection and quantification. This review summarizes the mechanisms by which miRNAs can control normal cellular processes and gene expression and the association of some circulating miRNAs with the incidence of different diseases, particularly essential hypertension.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90891951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-01DOI: 10.21608/RPBS.2018.5518.1014
E. Ibrahim, Ghada M. Hadad, R. A. Salam, A. Ibrahim, Safwat Ahmed, M. Radwan, W. Gul, M. Elsohly
Cannabis sativa L. is annually herbal plant belonging to the family Cannabaceae gaining more and more attention all over the world, due to its long historical clinical practice and its recent legalization either for recreational or medicinal uses. The quality control of the plant is a critical for its legalization and globalization. This review deals with the recent analytical methods (years 2015-2018) in the quality control of Cannabis, including the traditional chromatographic methods like HPLC, LC/MS/MS, UPLC, GC/FID, and GC/MS; and spectrophotometric, including FT-IR, NIR and NMR techniques as well as the most recent advanced techniques. The comprehensive methods, such as fingerprint and multi-component quantification are emphasized; hyphenated techniques, like HPLC, LC/MS/MS, GC/FID, GC-MS, LC-NMR, chemometric methods. In few words, the analysis and quality control of cannabis biomass, extracts and/or preparations is moving towards an integrative and comprehensive direction, in order to better address the inherent holistic nature of cannabis.
{"title":"Recent Analytical Approaches in Quality Control of Cannabis sativa L. and Its Preparations","authors":"E. Ibrahim, Ghada M. Hadad, R. A. Salam, A. Ibrahim, Safwat Ahmed, M. Radwan, W. Gul, M. Elsohly","doi":"10.21608/RPBS.2018.5518.1014","DOIUrl":"https://doi.org/10.21608/RPBS.2018.5518.1014","url":null,"abstract":"Cannabis sativa L. is annually herbal plant belonging to the family Cannabaceae gaining more and more attention all over the world, due to its long historical clinical practice and its recent legalization either for recreational or medicinal uses. The quality control of the plant is a critical for its legalization and globalization. This review deals with the recent analytical methods (years 2015-2018) in the quality control of Cannabis, including the traditional chromatographic methods like HPLC, LC/MS/MS, UPLC, GC/FID, and GC/MS; and spectrophotometric, including FT-IR, NIR and NMR techniques as well as the most recent advanced techniques. The comprehensive methods, such as fingerprint and multi-component quantification are emphasized; hyphenated techniques, like HPLC, LC/MS/MS, GC/FID, GC-MS, LC-NMR, chemometric methods. In few words, the analysis and quality control of cannabis biomass, extracts and/or preparations is moving towards an integrative and comprehensive direction, in order to better address the inherent holistic nature of cannabis.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85079183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-01DOI: 10.21608/RPBS.2018.23373
I. Salem, S. Mostafa, Osama I. El-Sabbag, T. Ibrahim, Ahmed Abdulrahmanf, M. M. Bendary
The aim of this study is to synthesize new benzenesulfonamide derivatives that possess antibacterial activity using Bumetanide as a precursor of benzenesulfonamide. A novel series of hydrazones containing benzenesulfonamides (4a-i) and 1,3,4 oxadiazole containing benzenesulfonamides (5a-e) were prepared and their antibacterial and antifungal activities were measured using microplate broth assay against Gram-positive: Staphylococcus aureus, Gram-negative: Escherichia coli and Pseudomonas aeruginosa and Fungi: Aspergillus fumigates and Candida albicans. The results showed that some of the prepared compounds exhibit a good to excellent activity against the mentioned bacteria, however all the series showed no activity against fungi. Among the series, Compound (4i) demoed an excellent antibacterial activity better than that of the standard reference (Sulfamethoxazole) against Escherichia coli, while compounds (4c, 4e, 4g) showed good activity against all tested bacterial strains equals to Sulfamethoxazole activity. Moreover, compounds (4g, 5d) exert an antimicrobial activity equals to that of Sulfamethoxazole against Pseudomonas aeruginosa, while compounds (4a, 4b, 4c, 4f, 4g, 4h) exhibit similar activity to Sulfamethoxazole against Escherichia coli.
{"title":"Synthesis and Antimicrobial Evaluation of Novel Hydrazones and 1,3,4-Oxadiazoles Incorporating Bumetanide Derivatives","authors":"I. Salem, S. Mostafa, Osama I. El-Sabbag, T. Ibrahim, Ahmed Abdulrahmanf, M. M. Bendary","doi":"10.21608/RPBS.2018.23373","DOIUrl":"https://doi.org/10.21608/RPBS.2018.23373","url":null,"abstract":"The aim of this study is to synthesize new benzenesulfonamide derivatives that possess antibacterial activity using Bumetanide as a precursor of benzenesulfonamide. A novel series of hydrazones containing benzenesulfonamides (4a-i) and 1,3,4 oxadiazole containing benzenesulfonamides (5a-e) were prepared and their antibacterial and antifungal activities were measured using microplate broth assay against Gram-positive: Staphylococcus aureus, Gram-negative: Escherichia coli and Pseudomonas aeruginosa and Fungi: Aspergillus fumigates and Candida albicans. The results showed that some of the prepared compounds exhibit a good to excellent activity against the mentioned bacteria, however all the series showed no activity against fungi. Among the series, Compound (4i) demoed an excellent antibacterial activity better than that of the standard reference (Sulfamethoxazole) against Escherichia coli, while compounds (4c, 4e, 4g) showed good activity against all tested bacterial strains equals to Sulfamethoxazole activity. Moreover, compounds (4g, 5d) exert an antimicrobial activity equals to that of Sulfamethoxazole against Pseudomonas aeruginosa, while compounds (4a, 4b, 4c, 4f, 4g, 4h) exhibit similar activity to Sulfamethoxazole against Escherichia coli.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79662529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-01DOI: 10.21608/rpbs.2018.5223.1011
Samar H. Faheim, A. Gardouh, A. Nouh, M. Ghorab
{"title":"Review article on nanoemulsions and nanostructured lipid carriers","authors":"Samar H. Faheim, A. Gardouh, A. Nouh, M. Ghorab","doi":"10.21608/rpbs.2018.5223.1011","DOIUrl":"https://doi.org/10.21608/rpbs.2018.5223.1011","url":null,"abstract":"","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84351024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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