Pub Date : 2019-05-14DOI: 10.21608/RPBS.2019.10975.1024
Mohammed Salah Ali
The majority of human microbiota resides in the GIT approximately 3.6 x 1013. Identification of gut microbiota composition in obese individuals and defining bacterial community differences between men and females help in the treatment of certain metabolic disorders. The analysis of gut microbiota of men and females was performed with 16s rRNA genes using Illumina MiSeq sequencing. 5 stool samples for each group were collected from obese men and females. In our study, we found that the Alpha diversity was enriched in obese men than obese females this may be due to the hormonal disturbance in the females which make the change in immunity and thereby, change in richness and diversity of gut microbiota composition. Firmicutes / Bacteroidetes ratio was higher in men than females due to the overrepresentation of Firmicutes in men and overrepresentation of Bacteroidetes in case of obese females. Finally, Illumina MiSeq sequencing of 16S rRNA V4 region was allowing cheap and efficient studying of gut microbiota.
大多数人类微生物群存在于胃肠道中，约为3.6 x 1013。确定肥胖个体的肠道微生物群组成和确定男性和女性之间的细菌群落差异有助于治疗某些代谢紊乱。使用Illumina MiSeq测序技术对男性和女性的肠道微生物群进行16s rRNA基因分析。每组分别从肥胖男性和女性中采集5份粪便样本。在我们的研究中，我们发现肥胖男性的α多样性比肥胖女性丰富，这可能是由于女性体内的激素干扰导致免疫力的改变，从而改变了肠道微生物群组成的丰富度和多样性。男性中厚壁菌门/拟杆菌门的比例高于女性，这是由于男性中厚壁菌门的比例过高，而肥胖女性中拟杆菌门的比例过高。最后，Illumina MiSeq对16S rRNA V4区域进行测序，使肠道微生物群的研究变得廉价高效。
{"title":"Gender dependent gut microbiome in obese Egyptian individuals","authors":"Mohammed Salah Ali","doi":"10.21608/RPBS.2019.10975.1024","DOIUrl":"https://doi.org/10.21608/RPBS.2019.10975.1024","url":null,"abstract":"The majority of human microbiota resides in the GIT approximately 3.6 x 1013. Identification of gut microbiota composition in obese individuals and defining bacterial community differences between men and females help in the treatment of certain metabolic disorders. The analysis of gut microbiota of men and females was performed with 16s rRNA genes using Illumina MiSeq sequencing. 5 stool samples for each group were collected from obese men and females. In our study, we found that the Alpha diversity was enriched in obese men than obese females this may be due to the hormonal disturbance in the females which make the change in immunity and thereby, change in richness and diversity of gut microbiota composition. Firmicutes / Bacteroidetes ratio was higher in men than females due to the overrepresentation of Firmicutes in men and overrepresentation of Bacteroidetes in case of obese females. Finally, Illumina MiSeq sequencing of 16S rRNA V4 region was allowing cheap and efficient studying of gut microbiota.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89650454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-14DOI: 10.21608/RPBS.2019.11644.1029
A. Badawy
Drug discovery and development has a long history and dates back to the early days of human civilization. In those ancient times, drugs were not just used as physical remedies but were also associated with religious and spiritual healing. Thymelaeaceae family have been the subject of numerous phytochemical studies. Initially, interest may have been due to the marked toxicity of these plants, but the widespread use of some species medicinally was certainly a reason for this interest. Thymelaea hirsuta is a perennial, evergreen and dioecious shrub, which is native to North Africa. Thymelaea hirsuta is a widespread invasive weed, is commonly known as “Methnane” and has been used traditionally as having antiseptic, antimelanogenesis, antioxidant and diabetic. There are numerous phytochemical studies lead to its richness in a variety of different classes of natural products, especially, coumarins, lignans, steroids and flavones. In addition, it used as a new fiber source for handmade paper. Therefore, in this review, we have mainly summarized the recent chemical constituent's isolated and biological activity identified from Thymelaea hirsuta. We hope that this review will provide further information for understanding the expected isolated compounds and its possible potent activity.
{"title":"Review article on Chemical constituents and Biological activity of Thymelaea hirsuta","authors":"A. Badawy","doi":"10.21608/RPBS.2019.11644.1029","DOIUrl":"https://doi.org/10.21608/RPBS.2019.11644.1029","url":null,"abstract":"Drug discovery and development has a long history and dates back to the early days of human civilization. In those ancient times, drugs were not just used as physical remedies but were also associated with religious and spiritual healing. Thymelaeaceae family have been the subject of numerous phytochemical studies. Initially, interest may have been due to the marked toxicity of these plants, but the widespread use of some species medicinally was certainly a reason for this interest. Thymelaea hirsuta is a perennial, evergreen and dioecious shrub, which is native to North Africa. Thymelaea hirsuta is a widespread invasive weed, is commonly known as “Methnane” and has been used traditionally as having antiseptic, antimelanogenesis, antioxidant and diabetic. There are numerous phytochemical studies lead to its richness in a variety of different classes of natural products, especially, coumarins, lignans, steroids and flavones. In addition, it used as a new fiber source for handmade paper. Therefore, in this review, we have mainly summarized the recent chemical constituent's isolated and biological activity identified from Thymelaea hirsuta. We hope that this review will provide further information for understanding the expected isolated compounds and its possible potent activity.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83385400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.21608/rpbs.2019.66749
S. Farahat, R. A. Salam, Ghada M. Hadad, A. El-Gindy, Tamer H. Hassan
Green liquid chromatographic method using cyano column and ethanol and 0.5% acetic acid in water (pH 2.8) as mobile phase in gradient mode has been developed for analysis of Amoxicillin, Metronidazole and Ciprofloxacin in human urine. Quantification was carried out using a diode-array UV detector. The detection wavelength was 255 nm. The retention times and detection limits for each antibiotic were 4.9 min and 0.5 μg/mL for Amoxicillin, 6.035 min and 0.14 μg/mL for Metronidazole, 8.03 min and 3.07 μg/mL for Ciprofloxacin, respectively. The developed method was applied to examine the urinary excretion pattern of Amoxicillin, Metronidazole and Ciprofloxacin in healthy male volunteer after an oral administration of Metronidazole and Ciprofloxacin in their combination tablets (Ciprodiazole) and combination tablets of Ciprofloxacin and Amoxicillin (Helicocin). The developed HPLC method was successfully used for the analysis of the selected drugs in their dosage forms and human urine without interference from the excipients or urine matrix. The proposed method was rapid, specific, precise, accurate, environmentally friendly and suitable for bioequivalence and pharmacokinetic studies.
{"title":"An ecofriendly green liquid chromatographic method for simultaneous determination of Amoxicillin, Metronidazole and Ciprofloxacin; application to dosage form and human urine","authors":"S. Farahat, R. A. Salam, Ghada M. Hadad, A. El-Gindy, Tamer H. Hassan","doi":"10.21608/rpbs.2019.66749","DOIUrl":"https://doi.org/10.21608/rpbs.2019.66749","url":null,"abstract":"Green liquid chromatographic method using cyano column and ethanol and 0.5% acetic acid in water (pH 2.8) as mobile phase in gradient mode has been developed for analysis of Amoxicillin, Metronidazole and Ciprofloxacin in human urine. Quantification was carried out using a diode-array UV detector. The detection wavelength was 255 nm. The retention times and detection limits for each antibiotic were 4.9 min and 0.5 μg/mL for Amoxicillin, 6.035 min and 0.14 μg/mL for Metronidazole, 8.03 min and 3.07 μg/mL for Ciprofloxacin, respectively. The developed method was applied to examine the urinary excretion pattern of Amoxicillin, Metronidazole and Ciprofloxacin in healthy male volunteer after an oral administration of Metronidazole and Ciprofloxacin in their combination tablets (Ciprodiazole) and combination tablets of Ciprofloxacin and Amoxicillin (Helicocin). The developed HPLC method was successfully used for the analysis of the selected drugs in their dosage forms and human urine without interference from the excipients or urine matrix. The proposed method was rapid, specific, precise, accurate, environmentally friendly and suitable for bioequivalence and pharmacokinetic studies.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75833366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.21608/rpbs.2019.5575.1015
S. Antar, M. El-Azab, Reem M Hazem, M. Saleh
Breast cancer is considered as the most familiar cancer in females which represented 38.8 % in Egypt and 29% in the world. It is the second common cause of cancer-related death in women. Treatment of breast cancer with Doxorubicin may lead to many side effects, mainly cardiac and renal fibrosis. The present study aimed to investigate the underlying molecular mechanisms for the potential anti-fibrotic effect of pirfenidone (500mg/kg, P.O. once daily) and Vitamin D (0.5μg/kg I.P. once daily) against doxorubicin (15 mg/kg I.P.) induced cardioand renalfibrosis. Moreover, the anti-cancer potential of pirfenidone (PFD) and Vitamin D either alone or in combination with doxorubicin will be assessed in a xenograft experimental model of breast cancer. Then, tissue and blood samples will be collected after two weeks posttreatment to assess the toxicity of Doxorubicin.
{"title":"Investigating Pirfenidone and Vitamin D for Targeting Cardiac and Renal Fibrotic Pathways in Experimentally-Induced Animal Model","authors":"S. Antar, M. El-Azab, Reem M Hazem, M. Saleh","doi":"10.21608/rpbs.2019.5575.1015","DOIUrl":"https://doi.org/10.21608/rpbs.2019.5575.1015","url":null,"abstract":"Breast cancer is considered as the most familiar cancer in females which represented 38.8 % in Egypt and 29% in the world. It is the second common cause of cancer-related death in women. Treatment of breast cancer with Doxorubicin may lead to many side effects, mainly cardiac and renal fibrosis. The present study aimed to investigate the underlying molecular mechanisms for the potential anti-fibrotic effect of pirfenidone (500mg/kg, P.O. once daily) and Vitamin D (0.5μg/kg I.P. once daily) against doxorubicin (15 mg/kg I.P.) induced cardioand renalfibrosis. Moreover, the anti-cancer potential of pirfenidone (PFD) and Vitamin D either alone or in combination with doxorubicin will be assessed in a xenograft experimental model of breast cancer. Then, tissue and blood samples will be collected after two weeks posttreatment to assess the toxicity of Doxorubicin.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87784014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.21608/RPBS.2019.9792.1021
aya ahmed mousa, A. Abdel-Rahman, M. Azab
A number of methods of sampling the vagina for the presence of candida have been developed. Such techniques play an important role in the diagnosis and management of vaginal candidiasis. In the past, identification of candida isolated from the vagina has usually been limited to the genus Candida or to the species C. albicans. However, with the recognition that Candida species differ in the production of putative virulence factors and sensitivity to antifungal agents, greater emphasis has been placed on identification of isolates to species level. As a result, a range of commercially available systems for yeast identification can now be used in conjunction with traditional identification procedures. In this work, Candida detected by many phenotypic identification methods such as sabaroud dextrose agar medium, microscopical identification, sugar fermentation test and germ tube test. Polymerase chain reaction (PCR) also used as a fast and sensitive genotypic technic to identify Candida sp.
{"title":"Identification methods of Candida isolated from vaginal infections","authors":"aya ahmed mousa, A. Abdel-Rahman, M. Azab","doi":"10.21608/RPBS.2019.9792.1021","DOIUrl":"https://doi.org/10.21608/RPBS.2019.9792.1021","url":null,"abstract":"A number of methods of sampling the vagina for the presence of candida have been developed. Such techniques play an important role in the diagnosis and management of vaginal candidiasis. In the past, identification of candida isolated from the vagina has usually been limited to the genus Candida or to the species C. albicans. However, with the recognition that Candida species differ in the production of putative virulence factors and sensitivity to antifungal agents, greater emphasis has been placed on identification of isolates to species level. As a result, a range of commercially available systems for yeast identification can now be used in conjunction with traditional identification procedures. In this work, Candida detected by many phenotypic identification methods such as sabaroud dextrose agar medium, microscopical identification, sugar fermentation test and germ tube test. Polymerase chain reaction (PCR) also used as a fast and sensitive genotypic technic to identify Candida sp.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72875957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.21608/RPBS.2019.7002.1020
M. Ramadan, S. Solyman, M. Yones, Hamada Halaby, Yaser Abdalla, Amr Hanora
Atopic dermatitis (AD) is a chronic complex inflammatory skin disorder. AD characterized by multiple contributing factors, such as impairment in skin barrier integrity, participation of either innate or adaptive immunity arms, in addition to the pivotal roles of cutaneous microbiome. The analysis of cutaneous microbiota was performed using shotgun metagenomic sequencing on an Illumina HiSeq platform in parallel with 16s rRNA genes using MiSeq sequencing. Two samples for each sequencing technique were collected from one child and one adult subjects with AD. Metagenomic data from 16srRNA was in accordance with those from shotgun sequencing. Taxonomic profile of samples at genus level showed overrepresentation of Streptococcus in child and Staphylococcus in adults. Species level analysis of reads revealed monoclonality of Staphylococcus aureus community in comparison to heterogeneity of Staphylococcus epidermidis. 16S rRNA gene based analysis was unable to provide comprehensive description of bacterial communities, while shotgun metagenomic analyses described the composition of microbiota with high resolution at different taxonomic levels especially subspecies and single nucleotide variants levels. Finally, further studies using shotgun metagenomic approaches will be required for characterization of microbiome in healthy and diseased subjects.
{"title":"Shotgun Metagenomic analysis of cutaneous microbiome in patients with atopic dermatitis","authors":"M. Ramadan, S. Solyman, M. Yones, Hamada Halaby, Yaser Abdalla, Amr Hanora","doi":"10.21608/RPBS.2019.7002.1020","DOIUrl":"https://doi.org/10.21608/RPBS.2019.7002.1020","url":null,"abstract":"Atopic dermatitis (AD) is a chronic complex inflammatory skin disorder. AD characterized by multiple contributing factors, such as impairment in skin barrier integrity, participation of either innate or adaptive immunity arms, in addition to the pivotal roles of cutaneous microbiome. The analysis of cutaneous microbiota was performed using shotgun metagenomic sequencing on an Illumina HiSeq platform in parallel with 16s rRNA genes using MiSeq sequencing. Two samples for each sequencing technique were collected from one child and one adult subjects with AD. Metagenomic data from 16srRNA was in accordance with those from shotgun sequencing. Taxonomic profile of samples at genus level showed overrepresentation of Streptococcus in child and Staphylococcus in adults. Species level analysis of reads revealed monoclonality of Staphylococcus aureus community in comparison to heterogeneity of Staphylococcus epidermidis. 16S rRNA gene based analysis was unable to provide comprehensive description of bacterial communities, while shotgun metagenomic analyses described the composition of microbiota with high resolution at different taxonomic levels especially subspecies and single nucleotide variants levels. Finally, further studies using shotgun metagenomic approaches will be required for characterization of microbiome in healthy and diseased subjects.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75713649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.21608/RPBS.2019.6684.1019
R. A. Salam, Ghada M. Hadad, D. Abo-El-Matty, Mohamed Abdel Shakour
The production of counterfeit or substandard drugs is a widespread problem which can no longer be ignored. The spread of counterfeit drugs not only in different developing countries also it was detected in developed countries as well which may take part in morbidity, mortality, drug resistance, toxicity and loss of confidence in health-care systems. Moreover effort is urgently required on the legal, enforcement and scientific levels to over come this rising problem. Reliable analysis of the suspect products with several techniques, essentially based on chromatography and spectroscopy, are now at the disposal of the analysts to enable the distinction between genuine and counterfeit products. However the determination of the components and the use of analytical data for forensic purposes still constitute a challenge. The aim of this review article is therefore to point out the intricacy of medicine counterfeiting, and its risks also its impact so that a better understanding can provide solutions to fight more efficiently against it
{"title":"Pharmaceutical Counterfeit drugs: (The growing threat)","authors":"R. A. Salam, Ghada M. Hadad, D. Abo-El-Matty, Mohamed Abdel Shakour","doi":"10.21608/RPBS.2019.6684.1019","DOIUrl":"https://doi.org/10.21608/RPBS.2019.6684.1019","url":null,"abstract":"The production of counterfeit or substandard drugs is a widespread problem which can no longer be ignored. The spread of counterfeit drugs not only in different developing countries also it was detected in developed countries as well which may take part in morbidity, mortality, drug resistance, toxicity and loss of confidence in health-care systems. Moreover effort is urgently required on the legal, enforcement and scientific levels to over come this rising problem. Reliable analysis of the suspect products with several techniques, essentially based on chromatography and spectroscopy, are now at the disposal of the analysts to enable the distinction between genuine and counterfeit products. However the determination of the components and the use of analytical data for forensic purposes still constitute a challenge. The aim of this review article is therefore to point out the intricacy of medicine counterfeiting, and its risks also its impact so that a better understanding can provide solutions to fight more efficiently against it","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"575 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87263452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.21608/RPBS.2019.11635.1028
Hala H Zaatout
Delayed pregnancy is one of the main problems that may threaten the relationship between married people especially in Arab region. Finding a cure for this problem started after a period of time after the marriage without pregnancy. In some cases, herbs from tradition can be used in order to promote fertility and stimulate pregnancy. There are limited data for herbal remedies used by women in KSA that is concerned with pregnancy. This study was aimed at determining the prevalence rate and kind of herbal remedies used among female culture. First, a pre-structured questionnaire was administered among 430 Saudi females which revealed that 25% of them have used traditional herbals as a tool for enhancing the fertility. Then asking apothecary and dealers in that field, collecting samples and data were recorded. Finally, investigating the data collected in attempts to evaluate the efficacy and safety of such herbs and herbal mixtures. Rose of Jericho, Juniper, Myrrh, Black cumin and garden cress were the most commonly used remedies recorded from respondents. This study focuses on possible warning and precaution. Recommendations were suggested to control any hazards.
{"title":"Insight in some herbals and herbal combinations used by Saudi females for improving fertility and enhance pregnancy","authors":"Hala H Zaatout","doi":"10.21608/RPBS.2019.11635.1028","DOIUrl":"https://doi.org/10.21608/RPBS.2019.11635.1028","url":null,"abstract":"Delayed pregnancy is one of the main problems that may threaten the relationship between married people especially in Arab region. Finding a cure for this problem started after a period of time after the marriage without pregnancy. In some cases, herbs from tradition can be used in order to promote fertility and stimulate pregnancy. There are limited data for herbal remedies used by women in KSA that is concerned with pregnancy. This study was aimed at determining the prevalence rate and kind of herbal remedies used among female culture. First, a pre-structured questionnaire was administered among 430 Saudi females which revealed that 25% of them have used traditional herbals as a tool for enhancing the fertility. Then asking apothecary and dealers in that field, collecting samples and data were recorded. Finally, investigating the data collected in attempts to evaluate the efficacy and safety of such herbs and herbal mixtures. Rose of Jericho, Juniper, Myrrh, Black cumin and garden cress were the most commonly used remedies recorded from respondents. This study focuses on possible warning and precaution. Recommendations were suggested to control any hazards.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79594341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.21608/RPBS.2019.9807.1022
E. Abdelrazik, M. El-hamahmy, Enas E Eltamany, Islam hassan Abuelsoud, E. Ali, Akram Abu-seidah
The effect of different concentrations of NaCl (6, 8 and 10 dS/m) on growth and alkaloids production in 21 days-old callus of Egyptian henbane (Hyoscyamu muticus L.) was studied. Results showed that 10 dS/m of NaCl increased fresh and dry weight by 4.1 and 2.8 fold more than control, respectively. Two fold Total alkaloids increased by 2 fold at 10 dS/m compared to control or wild leaves. In contrary, wild leaves had the highest amount of hyosyamine (2.8 mg/g DW) followed by 6 dS/m stressed-callus (1.14 mg/g DW) as detected by HPTLC. Peroxidase and superoxide dismutase activity were increased by 2.6 and 2.3 fold, respectively in highly salt stressed callus than control while catalase activity (CAT) was decreased. Anthocyanin concentration was increased 3 fold in 10 dS/m of NaCl stressed callus compared to control. Glutathione content was increased by 33.8% under low level of NaCl more than control. Reduction of photosynthetic pigments under all NaCl levels led to decrease the total carbohydrate and protein content. Protein band with molecular weight 109 KDa was a unique band in 10dS/m of NaCl treated callus with high expression of protein bands with MW 44, 38 and 32. Histologically, callus cells under high level of NaCl had amoeboid shape and start in senescence with formation of lysiogenous intercellular spaces.
{"title":"Enhancement of Growth and Alkaloids Accumulation in Hyoscyamus muticus L. Callus Cultures by High Salt Concentration","authors":"E. Abdelrazik, M. El-hamahmy, Enas E Eltamany, Islam hassan Abuelsoud, E. Ali, Akram Abu-seidah","doi":"10.21608/RPBS.2019.9807.1022","DOIUrl":"https://doi.org/10.21608/RPBS.2019.9807.1022","url":null,"abstract":"The effect of different concentrations of NaCl (6, 8 and 10 dS/m) on growth and alkaloids production in 21 days-old callus of Egyptian henbane (Hyoscyamu muticus L.) was studied. Results showed that 10 dS/m of NaCl increased fresh and dry weight by 4.1 and 2.8 fold more than control, respectively. Two fold Total alkaloids increased by 2 fold at 10 dS/m compared to control or wild leaves. In contrary, wild leaves had the highest amount of hyosyamine (2.8 mg/g DW) followed by 6 dS/m stressed-callus (1.14 mg/g DW) as detected by HPTLC. Peroxidase and superoxide dismutase activity were increased by 2.6 and 2.3 fold, respectively in highly salt stressed callus than control while catalase activity (CAT) was decreased. Anthocyanin concentration was increased 3 fold in 10 dS/m of NaCl stressed callus compared to control. Glutathione content was increased by 33.8% under low level of NaCl more than control. Reduction of photosynthetic pigments under all NaCl levels led to decrease the total carbohydrate and protein content. Protein band with molecular weight 109 KDa was a unique band in 10dS/m of NaCl treated callus with high expression of protein bands with MW 44, 38 and 32. Histologically, callus cells under high level of NaCl had amoeboid shape and start in senescence with formation of lysiogenous intercellular spaces.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"125 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80180919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.21608/RPBS.2019.5244.1012
A. Youssef, E. Mehanna, D. Abo-Elmatty
Diabetes Mellitus (D.M) has reached epidemic proportion and has become one of the most challenging health problems of the 21st century. Moreover, it is the 4th leading cause of death by disease globally, where every 10 seconds a person dies from diabetes-related causes. Obesity, insulin resistance, dyslipidemia and hypertension are associated with (T2DM) termed together as a metabolic syndrome. Mobilization of free fatty acids and secretion of certain inflammatory adipokines from adipose tissue promote insulin resistance in obese diabetics by interfere with insulin sensitivity, glucose and lipid metabolism. Chemerin, a specific adipokine, has an essential role in glucose and lipid metabolism. It plays a role in pathogenesis of obesity and T2DM also has important roles in energy metabolism, adipogenesis, and inflammation. Asymmetric dimethyl arginine (ADMA) consider a natural analogue of the essential amino acid arginine and a metabolic by-product obtained from processes of protein turnover of all human cells cytoplasm.
{"title":"Levels of chemerin and asymmetric dimethylarginine (ADMA) in obese type 2 diabetic patients.","authors":"A. Youssef, E. Mehanna, D. Abo-Elmatty","doi":"10.21608/RPBS.2019.5244.1012","DOIUrl":"https://doi.org/10.21608/RPBS.2019.5244.1012","url":null,"abstract":"Diabetes Mellitus (D.M) has reached epidemic proportion and has become one of the most challenging health problems of the 21st century. Moreover, it is the 4th leading cause of death by disease globally, where every 10 seconds a person dies from diabetes-related causes. Obesity, insulin resistance, dyslipidemia and hypertension are associated with (T2DM) termed together as a metabolic syndrome. Mobilization of free fatty acids and secretion of certain inflammatory adipokines from adipose tissue promote insulin resistance in obese diabetics by interfere with insulin sensitivity, glucose and lipid metabolism. Chemerin, a specific adipokine, has an essential role in glucose and lipid metabolism. It plays a role in pathogenesis of obesity and T2DM also has important roles in energy metabolism, adipogenesis, and inflammation. Asymmetric dimethyl arginine (ADMA) consider a natural analogue of the essential amino acid arginine and a metabolic by-product obtained from processes of protein turnover of all human cells cytoplasm.","PeriodicalId":21118,"journal":{"name":"Records of Pharmaceutical and Biomedical Sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90888770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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