Prostaglandins & other lipid mediators最新文献_第7页

Protection by selective mTORC2 inhibition of Zymosan-induced hypotension and systemic inflammation mediated via IKKα/IκB-α/NF-κB activation 选择性 mTORC2 抑制通过 IKKα/IκB-α/NF-κB 激活对 Zymosan 引起的低血压和全身炎症的保护作用。

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-10-24 DOI: 10.1016/j.prostaglandins.2024.106918

Zainab Sabrie , Meryem Temiz-Resitoglu , Taskin Kalkan , Banu Kilic , Bahar Tunctan , Kafait U. Malik , Seyhan Sahan-Firat

Non-septic shock is a serious condition leading to multiple organ dysfunction. Although targeting the mammalian target of the rapamycin complex 1 (mTORC1) signaling pathway exerts potent anti-inflammatory activity, little is known about mTORC2’s contribution to non-septic shock. Thus, our research aims to investigate mTORC2's contribution and associated changes of IκB kinase (IKKα)/inhibitor κB (IκB-α)/nuclear factor-ĸB (NF-κB) pathway on Zymosan (ZYM)-induced non-septic rat model using the novel mTORC2 selective inhibitor JR-AB2–011. Rats were given saline (4 ml/kg), dimethylsulfoxide (DMSO) (4 ml/kg), ZYM (500 mg/kg), and (or) JR-AB2–011 (1 mg/kg). Mean arterial pressure (MAP) and heart rate (HR) of rats were recorded. JR-AB2–011 reversed both ZYM-induced reduction in MAP and increase in HR. Protein expression and/or phosphorylation of rictor, protein kinase B (Akt), IκB-α, IKKα, NF-κB p65, inducible nitric oxide synthase (iNOS), nitrotyrosine, cyclooxygenase 2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, besides prostaglandin (PG) E₂ levels were measured. The enhanced expression of the proteins mentioned above has been inhibited by JR-AB2–011. These data suggest mTORC2’s promising role in ZYM-induced hypotension and systemic inflammation mediated via IKKα/IκB-α/NF-κB pathway.

非败血症性休克是一种导致多器官功能障碍的严重疾病。尽管针对哺乳动物雷帕霉素靶标复合体 1（mTORC1）信号通路可发挥强效抗炎活性，但人们对 mTORC2 在非失血性休克中的作用知之甚少。因此，我们的研究旨在利用新型 mTORC2 选择性抑制剂 JR-AB2-011，研究 mTORC2 对齐莫散（ZYM）诱导的非败血症大鼠模型的贡献以及 IκB 激酶（IKKα）/抑制因子κB（IκB-α）/核因子ĸB（NF-κB）通路的相关变化。给大鼠注射生理盐水（4 毫升/千克）、二甲基亚砜（DMSO）（4 毫升/千克）、ZYM（500 毫克/千克）和（或）JR-AB2-011（1 毫克/千克）。记录大鼠的平均动脉压（MAP）和心率（HR）。JR-AB2-011 逆转了 ZYM 诱导的 MAP 降低和 HR 升高。对 rictor、蛋白激酶 B (Akt)、IκB-α、IKKα、NF-κB p65、诱导型一氧化氮合酶 (iNOS)、硝基酪氨酸、环氧化酶 2 (COX-2)、肿瘤坏死因子 (TNF)-α、白细胞介素 (IL)-1β 以及前列腺素 (PG) E2 水平的蛋白表达和/或磷酸化进行了测定。JR-AB2-011 可抑制上述蛋白的表达。这些数据表明，mTORC2 在通过 IKKα/IκB-α/NF-κB 通路介导的 ZYM 诱导的低血压和全身性炎症中发挥着重要作用。

{"title":"Protection by selective mTORC2 inhibition of Zymosan-induced hypotension and systemic inflammation mediated via IKKα/IκB-α/NF-κB activation","authors":"Zainab Sabrie , Meryem Temiz-Resitoglu , Taskin Kalkan , Banu Kilic , Bahar Tunctan , Kafait U. Malik , Seyhan Sahan-Firat","doi":"10.1016/j.prostaglandins.2024.106918","DOIUrl":"10.1016/j.prostaglandins.2024.106918","url":null,"abstract":"<div><div>Non-septic shock is a serious condition leading to multiple organ dysfunction. Although targeting the mammalian target of the rapamycin complex 1 (mTORC1) signaling pathway exerts potent anti-inflammatory activity, little is known about mTORC2’s contribution to non-septic shock. Thus, our research aims to investigate mTORC2's contribution and associated changes of IκB kinase (IKKα)/inhibitor κB (IκB-α)/nuclear factor-ĸB (NF-κB) pathway on Zymosan (ZYM)-induced non-septic rat model using the novel mTORC2 selective inhibitor JR-AB2–011. Rats were given saline (4 ml/kg), dimethylsulfoxide (DMSO) (4 ml/kg), ZYM (500 mg/kg), and (or) JR-AB2–011 (1 mg/kg). Mean arterial pressure (MAP) and heart rate (HR) of rats were recorded. JR-AB2–011 reversed both ZYM-induced reduction in MAP and increase in HR. Protein expression and/or phosphorylation of rictor, protein kinase B (Akt), IκB-α, IKKα, NF-κB p65, inducible nitric oxide synthase (iNOS), nitrotyrosine, cyclooxygenase 2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, besides prostaglandin (PG) E<sub>2</sub> levels were measured. The enhanced expression of the proteins mentioned above has been inhibited by JR-AB2–011. These data suggest mTORC2’s promising role in ZYM-induced hypotension and systemic inflammation mediated via IKKα/IκB-α/NF-κB pathway.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106918"},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of purslane (Portulaca oleracea) on C-reactive protein, lipid profile, and glycemic control in patients with type 2 diabetes mellitus: A GRADE-assessed systematic review and meta-analysis of randomized controlled trials 马齿苋对 2 型糖尿病患者 C 反应蛋白、血脂状况和血糖控制的影响：对随机对照试验进行 GRADE 评估的系统综述和荟萃分析。

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-10-23 DOI: 10.1016/j.prostaglandins.2024.106917

Guo Donglin , Roya Birjandi , Fatemeh Moghimi Esfandabadi , Amirreza Haedi , Chen Fujiang

Although numerous trials have demonstrated the benefits of purslane on patients with type 2 diabetes mellitus (T2DM), the results of some studies reject such effect. We elucidated the influence of purslane on c-reactive protein (CRP), lipid profile, and glycemic control in patients with T2DM. We conducted a systematic search on Web of Sciences, Scopus, the Cochrane Library, PubMed, and Embase, to identify studies published from their inception to February 10, 2024. The random effect model was used to calculate the combined effect, the weighted mean differences (WMDs) and its 95 % confidence interval (CI). Purslane supplementation significantly reduced fasting blood glucose (FBG) levels (WMD: −15.01; 95 % CI: −25.31, −4.71, p= 0.004), total cholesterol (TC) (WMD: −17.75; 95 % CI: −26.06, −9.45; p< 0.001), triglyceride (TG) (WMD: −21.30; 95 % CI: −32.59, −10.00; p< 0.001), low-density lipoprotein cholesterol (LDL-C) (WMD: −6.10; 95 % CI: −9.52, −2.68; p< 0.001), and CRP levels (WMD: −1.44; 95 % CI: −2.25, −0.63; p< 0.001), and increased high-density lipoprotein cholesterol (HDL-C) (WMD: 6.17; 95 % CI: 2.53, 9.80; p< 0.001). Also, purslane did not significant effect on serum levels of insulin, hemoglobin A1C (HbA1c), and homeostatic model Assessment for insulin resistance (HOMA-IR). The results of our study showed that purslane supplementation improved FBG, and lipid profile levels, but did not affect HOMA-IR, insulin, and HbA1c levels. However, high-quality trials are needed to approve our results.

尽管许多试验证明马齿苋对2型糖尿病（T2DM）患者有益，但一些研究结果却否定了这种作用。我们阐明了马齿苋对 T2DM 患者的 c 反应蛋白（CRP）、血脂状况和血糖控制的影响。我们在 Web of Sciences、Scopus、Cochrane Library、PubMed 和 Embase 上进行了系统检索，以确定从开始到 2024 年 2 月 10 日发表的研究。研究采用随机效应模型计算综合效应、加权平均差（WMD）及其95%置信区间（CI）。补充马齿苋可明显降低空腹血糖 (FBG) 水平（WMD：-15.01；95% CI：-25.31，-4.71，p= 0.004）、总胆固醇 (TC)（WMD：-17.75；95% CI：-26.06，-9.45；p< 0.001）、甘油三酯 (TG)（WMD：-21.30；95% CI：-32.59，-10.00；p< 0.001）、低密度脂蛋白胆固醇（LDL-C）（WMD：-6.10；95% CI：-9.52，-2.68；p< 0.001）和 CRP 水平（WMD：-1.44；95% CI：-2.25，-0.63；p< 0.001），并增加了高密度脂蛋白胆固醇（HDL-C）（WMD：6.17；95% CI：2.53，9.80；p< 0.001）。此外，马齿苋对血清中的胰岛素水平、血红蛋白A1C（HbA1c）和胰岛素抵抗的稳态模型评估（HOMA-IR）没有显著影响。我们的研究结果表明，补充马齿苋可改善血糖和血脂水平，但不会影响 HOMA-IR、胰岛素和 HbA1c 水平。然而，我们的研究结果还需要高质量的试验来验证。

{"title":"The effects of purslane (Portulaca oleracea) on C-reactive protein, lipid profile, and glycemic control in patients with type 2 diabetes mellitus: A GRADE-assessed systematic review and meta-analysis of randomized controlled trials","authors":"Guo Donglin , Roya Birjandi , Fatemeh Moghimi Esfandabadi , Amirreza Haedi , Chen Fujiang","doi":"10.1016/j.prostaglandins.2024.106917","DOIUrl":"10.1016/j.prostaglandins.2024.106917","url":null,"abstract":"<div><div>Although numerous trials have demonstrated the benefits of purslane on patients with type 2 diabetes mellitus (T2DM), the results of some studies reject such effect. We elucidated the influence of purslane on c-reactive protein (CRP), lipid profile, and glycemic control in patients with T2DM. We conducted a systematic search on Web of Sciences, Scopus, the Cochrane Library, PubMed, and Embase, to identify studies published from their inception to February 10, 2024. The random effect model was used to calculate the combined effect, the weighted mean differences (WMDs) and its 95 % confidence interval (CI). Purslane supplementation significantly reduced fasting blood glucose (FBG) levels (WMD: −15.01; 95 % CI: −25.31, −4.71, p= 0.004), total cholesterol (TC) (WMD: −17.75; 95 % CI: −26.06, −9.45; p< 0.001), triglyceride (TG) (WMD: −21.30; 95 % CI: −32.59, −10.00; p< 0.001), low-density lipoprotein cholesterol (LDL-C) (WMD: −6.10; 95 % CI: −9.52, −2.68; p< 0.001), and CRP levels (WMD: −1.44; 95 % CI: −2.25, −0.63; p< 0.001), and increased high-density lipoprotein cholesterol (HDL-C) (WMD: 6.17; 95 % CI: 2.53, 9.80; p< 0.001). Also, purslane did not significant effect on serum levels of insulin, hemoglobin A1C (HbA1c), and homeostatic model Assessment for insulin resistance (HOMA-IR). The results of our study showed that purslane supplementation improved FBG, and lipid profile levels, but did not affect HOMA-IR, insulin, and HbA1c levels. However, high-quality trials are needed to approve our results.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106917"},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of aged garlic extract on blood pressure in hypertensive patients: A systematic review and meta-analysis of randomized controlled trials 陈年大蒜提取物对高血压患者血压的影响：随机对照试验的系统回顾和荟萃分析。

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-10-20 DOI: 10.1016/j.prostaglandins.2024.106914

Mohamed J. Saadh , Muthena Kariem , Madhu Shukla , Suhas Ballal , Abhishek Kumar , Mamata Chahar , Suman Saini , Ish Kapila , Shirin Hasaanzadeh

Background

There is no consensus based on the existing literature regarding the effect of aged garlic extract (AGE) on blood pressure. The present systematic review and meta-analysis was conducted to synthesize existing data from randomized clinical trials (RCTs) concerning the impact of AGE supplementation on blood pressure in hypertensive patients.

Methods

We searched English web databases including, PubMed, Scopus, Embase, Web of Science until August 2024. Random-effects meta-analysis was used to pool weighted mean differences (WMD) and 95 % CI for the outcomes.

Results

Overall, nine RCTs with584 participants met inclusion criteria. AGE tea supplementation reduces systolic blood pressure (SBP) (WMD: −4.03; %95CI: −6.87, −1.20; I2: 57.1 %) and diastolic blood pressure (DBP) (WMD: −1.44; 95 % CI= −2.87, −0.02; P = 0.052; I²: 36.8 %; P: 0.105). Moreover, subgroup analysis indicated that higher doses of AGE supplementation in hypertensive patients significantly decreased DBP, and SBP.

Conclusion

The results of this study suggest that AGE supplements may be beneficial for improving blood pressure in hypertensive patients, but significant effects are observed only at doses over 1200 mg/day. To confirm these results, well-designed future trials will be needed.

背景：关于陈年大蒜提取物（AGE）对血压的影响，现有文献尚未达成共识。本系统综述和荟萃分析旨在综合现有随机临床试验（RCTs）中关于补充 AGE 对高血压患者血压影响的数据：我们检索了截至 2024 年 8 月的英文网络数据库，包括 PubMed、Scopus、Embase 和 Web of Science。采用随机效应荟萃分析法汇集结果的加权平均差（WMD）和 95% CI：共有9项研究符合纳入标准，584人参与研究。补充 AGE 茶可降低收缩压（SBP）（WMD：-4.03；95%CI：-6.87，-1.20；I2：57.1%）和舒张压（DBP）（WMD：-1.44；95%CI=-2.87，-0.02；P=0.052；I2：36.8%；P：0.105）。此外，亚组分析表明，高血压患者补充较高剂量的 AGE 可显著降低 DBP 和 SBP：本研究结果表明，AGE补充剂可能有益于改善高血压患者的血压，但只有在剂量超过1200毫克/天时才能观察到明显效果。要证实这些结果，还需要今后进行精心设计的试验。

{"title":"Effects of aged garlic extract on blood pressure in hypertensive patients: A systematic review and meta-analysis of randomized controlled trials","authors":"Mohamed J. Saadh , Muthena Kariem , Madhu Shukla , Suhas Ballal , Abhishek Kumar , Mamata Chahar , Suman Saini , Ish Kapila , Shirin Hasaanzadeh","doi":"10.1016/j.prostaglandins.2024.106914","DOIUrl":"10.1016/j.prostaglandins.2024.106914","url":null,"abstract":"<div><h3>Background</h3><div>There is no consensus based on the existing literature regarding the effect of aged garlic extract (AGE) on blood pressure. The present systematic review and meta-analysis was conducted to synthesize existing data from randomized clinical trials (RCTs) concerning the impact of AGE supplementation on blood pressure in hypertensive patients.</div></div><div><h3>Methods</h3><div>We searched English web databases including, PubMed, Scopus, Embase, Web of Science until August 2024. Random-effects meta-analysis was used to pool weighted mean differences (WMD) and 95 % CI for the outcomes.</div></div><div><h3>Results</h3><div>Overall, nine RCTs with584 participants met inclusion criteria. AGE tea supplementation reduces systolic blood pressure (SBP) (WMD: −4.03; %95CI: −6.87, −1.20; I2: 57.1 %) and diastolic blood pressure (DBP) (WMD: −1.44; 95 % CI= −2.87, −0.02; P = 0.052; I<sup>2</sup>: 36.8 %; P: 0.105). Moreover, subgroup analysis indicated that higher doses of AGE supplementation in hypertensive patients significantly decreased DBP, and SBP.</div></div><div><h3>Conclusion</h3><div>The results of this study suggest that AGE supplements may be beneficial for improving blood pressure in hypertensive patients, but significant effects are observed only at doses over 1200 mg/day. To confirm these results, well-designed future trials will be needed.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106914"},"PeriodicalIF":2.5,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of vitamin D supplementation on lipid profile, and hormonal functions in polycystic ovary syndrome: An umbrella systematic review and meta-analysis 维生素 D 补充剂对多囊卵巢综合征患者血脂和荷尔蒙功能的影响：总括性系统综述和荟萃分析。

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-10-09 DOI: 10.1016/j.prostaglandins.2024.106913

Tong Yin , Weixiong Lin , Kangwen Ming , Hnag Lv , Yinxin Wang , LI Yuanchao , Hao Zhen , Junyun Yuan , Hassan Asadi

Vitamin D has the potential to therapeutically affect the lipid profile and endocrine parameters of polycystic ovary syndrome (PCOS) patients. However, results from prior studies have been inconsistent. Therefore, we conducted an umbrella meta-analysis of randomized controlled trials (RCTs) to better understand the effectiveness of vitamin D in treating PCOS. We conducted an electronic search across multiple databases, including Embase, PubMed, Web of Science, Cochrane CENTRAL, and Scopus, from their inception to January 2, 2024. Random-effects models were used to perform the umbrella meta-analysis. The analysis included 15 meta-analyses of RCTs. Vitamin D demonstrated a significant reduction in TG levels (SMD = -0.23; 95 % CI: −0.42, −0.04, p = 0.02, and WMD = −8.76, 95 % CI: −11.81, −5.72; p <0.001), TC (SMD = −0.47, 95 % CI: −0.80, −0.13; p = 0.007, and WMD = −8.89, 95 % CI: −13.18, −4.59; p < 0.001), LDL-c (SMD = −0.24, 95 % CI: −0.38, −0.10; p = 0.001, and WMD = −3.83, 95 % CI: −6.49, −1.16; p = 0.005), TT (SMD = −0.15, 95 % CI: −0.29 to −0.01; p = 0.02), and DHEA (WMD: −28.03; 95 % CI: −56.9 to −0.36; p = 0.04). However, no significant effect on HDL-c, insulin, and BMI. The present meta-analysis revealed that vitamin D supplementation might significantly affect TG, TC, LDL-c, DHEA, and TT while it is not effective in improving BMI, HDL-c, and insulin. Vitamin D showed noteworthy effects in preventing lipid profile and enhancing hormonal function in patients with PCOS.

维生素 D 有可能对多囊卵巢综合症（PCOS）患者的血脂状况和内分泌参数产生治疗性影响。然而，之前的研究结果并不一致。因此，我们对随机对照试验（RCTs）进行了总括荟萃分析，以更好地了解维生素 D 治疗多囊卵巢综合征的效果。我们在多个数据库（包括 Embase、PubMed、Web of Science、Cochrane CENTRAL 和 Scopus）中进行了电子检索，检索时间从开始到 2024 年 1 月 2 日。采用随机效应模型进行总体荟萃分析。分析包括 15 项研究性临床试验的荟萃分析。维生素 D 能显著降低 TG 水平（SMD = -0.23；95% CI：-0.42，-0.04，p = 0.02；WMD = -8.76，95% CI：-11.81，-5.72；p = 0.05）。

{"title":"Effect of vitamin D supplementation on lipid profile, and hormonal functions in polycystic ovary syndrome: An umbrella systematic review and meta-analysis","authors":"Tong Yin , Weixiong Lin , Kangwen Ming , Hnag Lv , Yinxin Wang , LI Yuanchao , Hao Zhen , Junyun Yuan , Hassan Asadi","doi":"10.1016/j.prostaglandins.2024.106913","DOIUrl":"10.1016/j.prostaglandins.2024.106913","url":null,"abstract":"<div><div>Vitamin D has the potential to therapeutically affect the lipid profile and endocrine parameters of polycystic ovary syndrome (PCOS) patients. However, results from prior studies have been inconsistent. Therefore, we conducted an umbrella meta-analysis of randomized controlled trials (RCTs) to better understand the effectiveness of vitamin D in treating PCOS. We conducted an electronic search across multiple databases, including Embase, PubMed, Web of Science, Cochrane CENTRAL, and Scopus, from their inception to January 2, 2024. Random-effects models were used to perform the umbrella meta-analysis. The analysis included 15 meta-analyses of RCTs. Vitamin D demonstrated a significant reduction in TG levels (SMD = -0.23; 95 % CI: −0.42, −0.04, p = 0.02, and WMD = −8.76, 95 % CI: −11.81, −5.72; p <0.001), TC (SMD = −0.47, 95 % CI: −0.80, −0.13; p = 0.007, and WMD = −8.89, 95 % CI: −13.18, −4.59; p < 0.001), LDL-c (SMD = −0.24, 95 % CI: −0.38, −0.10; p = 0.001, and WMD = −3.83, 95 % CI: −6.49, −1.16; p = 0.005), TT (SMD = −0.15, 95 % CI: −0.29 to −0.01; p = 0.02), and DHEA (WMD: −28.03; 95 % CI: −56.9 to −0.36; p = 0.04). However, no significant effect on HDL-c, insulin, and BMI. The present meta-analysis revealed that vitamin D supplementation might significantly affect TG, TC, LDL-c, DHEA, and TT while it is not effective in improving BMI, HDL-c, and insulin. Vitamin D showed noteworthy effects in preventing lipid profile and enhancing hormonal function in patients with PCOS.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106913"},"PeriodicalIF":2.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of monoterpene molecules acts against 7KCh-mediated oxidative stress and neuroinflammatory amyloidogenic signalling pathways 单萜分子对 7KCh 介导的氧化应激和神经炎症性淀粉样蛋白生成信号通路具有治疗潜力。

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-09-28 DOI: 10.1016/j.prostaglandins.2024.106910

Mahalakshmi Krishnan , Manikandan Kumaresan , Sangeetha Ravi , Livya Catherene Martin , Parimalanandhini Duraisamy , Beulaja Manikandan , Arumugam Munusamy , Manikandan Ramar

Alzheimer’s disease (AD) is a degenerative disorder characterised by amyloid-beta aggregates activated by the accumulation of lipid molecules and their derivatives, especially 7-ketocholesterol (7KCh), an oxidised lipid that plays a great part in the progression of AD. The current therapeutics need bio-potential molecules and their biomedical application preventing 7KCh-induced cytotoxicity. In this study, bornyl acetate (BA) and menthol (ME), the natural monoterpenes were investigated for their neuroprotective effects against 7KCh-induced SH-SY5Y cells and their effects were compared to the standard drug galantamine (GA). 7KCh-induced changes like lipid accumulation, amyloid generation, free radical generation, acetylcholinesterase levels, calcium accumulation and mitochondrial membrane integrity were analysed in SH-SY5Y cells with or without BA and ME treatment. Furthermore, various mediators involved in the amyloidogenic, inflammatory and apoptotic pathways were studied. In our results, the cells induced with 7KCh upon co-treatment with BA and ME significantly reduced lipid accumulation and amyloid generation through toll-like receptor (TLR) 4 suppression and enhanced ATP binding cassette (ABCA) 1-mediated clearance. Co-treatment with BA and ME concurrently regulated oxidative stress, acetylcholinesterase activity, mitochondrial membrane potential and intracellular calcification altered by 7KCh-induced SH-SY5Y cells. Moreover, 7KCh-induced cells showed elevated mRNA levels of misfolded protein markers and apoptotic mediators which were significantly downregulated by BA and ME co-treatment. In addition, the protein expression of amyloidogenic, proinflammatory as well as pro-apoptotic markers was decreased by BA and ME co-treatment in 7KCh-induced cells. Overall, BA and ME mediated inhibition of amyloidogenic activation and cell survival against 7KCh-induced inflammation, thereby preventing the onset and progression of AD in comparison to GA.

阿尔茨海默病（AD）是一种退行性疾病，其特征是脂质分子及其衍生物，特别是氧化脂质 7-酮胆固醇（7KCh）的积聚激活了淀粉样蛋白-β 聚集，在阿尔茨海默病的发展过程中起着重要作用。目前的疗法需要一种具有生物潜力的分子及其生物医学应用，以防止 7KCh 引起的细胞毒性。在这项研究中，研究了天然单萜类化合物醋酸硼酯（BA）和薄荷醇（ME）对 7KCh 诱导的 SH-SY5Y 细胞的神经保护作用，并将它们的作用与标准药物加兰他敏（GA）进行了比较。研究分析了经 BA 和 ME 处理或未经 BA 和 ME 处理的 SH-SY5Y 细胞中 7KCh 诱导的变化，如脂质积累、淀粉样蛋白生成、自由基生成、乙酰胆碱酯酶水平、钙积累和线粒体膜完整性。此外，还研究了参与淀粉样蛋白生成、炎症和细胞凋亡途径的各种介质。我们的研究结果表明，用7KCh诱导的细胞经BA和ME联合处理后，通过抑制类毒素受体（TLR）4和提高ATP结合盒（ABCA）1介导的清除率，显著减少了脂质积累和淀粉样蛋白的生成。BA和ME同时处理可调节氧化应激、乙酰胆碱酯酶活性、线粒体膜电位和7KCh诱导的SH-SY5Y细胞所改变的细胞内钙化。此外，7KCh 处理的细胞显示错误折叠蛋白标记物和凋亡介质的 mRNA 水平升高，而 BA 和 ME 联合处理的细胞则显著降低了这些标记物和介质的 mRNA 水平。此外，BA 和 ME 联合处理 7KCh 诱导的细胞后，淀粉样蛋白、促炎症和促凋亡标志物的蛋白表达也有所下降。总之，BA 和 ME 可抑制淀粉样蛋白的活化，并使细胞存活，从而对抗 7KCh 诱导的炎症。此外，与GA相比，BA和ME在预防AD的发生和发展方面可能具有更好的效果。

{"title":"Therapeutic potential of monoterpene molecules acts against 7KCh-mediated oxidative stress and neuroinflammatory amyloidogenic signalling pathways","authors":"Mahalakshmi Krishnan , Manikandan Kumaresan , Sangeetha Ravi , Livya Catherene Martin , Parimalanandhini Duraisamy , Beulaja Manikandan , Arumugam Munusamy , Manikandan Ramar","doi":"10.1016/j.prostaglandins.2024.106910","DOIUrl":"10.1016/j.prostaglandins.2024.106910","url":null,"abstract":"<div><div>Alzheimer’s disease (AD) is a degenerative disorder characterised by amyloid-beta aggregates activated by the accumulation of lipid molecules and their derivatives, especially 7-ketocholesterol (7KCh), an oxidised lipid that plays a great part in the progression of AD. The current therapeutics need bio-potential molecules and their biomedical application preventing 7KCh-induced cytotoxicity. In this study, bornyl acetate (BA) and menthol (ME), the natural monoterpenes were investigated for their neuroprotective effects against 7KCh-induced SH-SY5Y cells and their effects were compared to the standard drug galantamine (GA). 7KCh-induced changes like lipid accumulation, amyloid generation, free radical generation, acetylcholinesterase levels, calcium accumulation and mitochondrial membrane integrity were analysed in SH-SY5Y cells with or without BA and ME treatment. Furthermore, various mediators involved in the amyloidogenic, inflammatory and apoptotic pathways were studied. In our results, the cells induced with 7KCh upon co-treatment with BA and ME significantly reduced lipid accumulation and amyloid generation through toll-like receptor (TLR) 4 suppression and enhanced ATP binding cassette (ABCA) 1-mediated clearance. Co-treatment with BA and ME concurrently regulated oxidative stress, acetylcholinesterase activity, mitochondrial membrane potential and intracellular calcification altered by 7KCh-induced SH-SY5Y cells. Moreover, 7KCh-induced cells showed elevated mRNA levels of misfolded protein markers and apoptotic mediators which were significantly downregulated by BA and ME co-treatment. In addition, the protein expression of amyloidogenic, proinflammatory as well as pro-apoptotic markers was decreased by BA and ME co-treatment in 7KCh-induced cells. Overall, BA and ME mediated inhibition of amyloidogenic activation and cell survival against 7KCh-induced inflammation, thereby preventing the onset and progression of AD in comparison to GA.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106910"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of raloxifene supplementation on blood pressure and Apo-lipoproteins in postmenopausal women: A systematic review and meta-analysis 补充雷洛昔芬对绝经后妇女血压和载脂蛋白的影响：系统回顾与元分析》。

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-09-27 DOI: 10.1016/j.prostaglandins.2024.106912

Nooshin Noshadi , Atefeh Bonyadian , Salehe Zarian , Fatemeh Kazemi , Melika Darzi , Farzaneh Akhavan Tabib , Mahdieh Abbasalizad-Farhangi , Beitullah Alipour , Sarehnaz Aghili

Introduction

Several studies indicated the ameliorating effects of raloxifene supplementation on apolipoproteins and blood pressure, although others have conflicting findings. Therefore, the present study was conducted in order to accurately and definitively understands the effect of raloxifene on apolipoprotein AI (Apo-AI), apolipoprotein B (APoB), lipoprotein (a) (Lp (a)), systolic blood pressure (SBP) and diastolic blood pressure (DBP) in postmenopausal women.

Methods

A systematic literature search was conducted using scientific databases including PubMed, Scopus, Embase, and Web of Science and the Cochrane Library, through May 2024. The quality of studies was assessed using Cochrane tool. Random-effects meta-analysis was used to pool standardized mean differences (SMD) and 95 % CI for the outcomes.

Results

Twenty trials, with interventions ranging from 6 to 144 weeks and 2825 participants, were included. Raloxifene supplementation demonstrated significant reductions in ApoB (SMD: −0.92; 95 % CI: −1.49 to −0.35; P = 0.001), and Lp (a) (SMD: −0.25; 95 % CI: −0.39 to −0.11; P < 0.001) while increasing Apo-AI levels (SMD: 0.29; 95 % CI: 0.22–0.36; P < 0.001). Conversely, no significant effects were observed on SBP (WMD: −0.49 mmHg; 95 % CI: −3.01–2.04; P = 0.706), and DBP (WMD: −0.81 mmHg; 95 % CI: −4.04–2.41; P = 0.621). Moreover, subgroup analysis indicated that raloxifene significantly decreased DBP in studies with intervention durations of >12 weeks.

Conclusions

This meta-analysis has shown that raloxifene supplementation may have beneficial effects on apolipoproteins in postmenopausal women. Future studies are needed to investigate the effect of raloxifene on health status in in postmenopausal women.

介绍：多项研究表明，补充雷洛昔芬可改善脂蛋白和血压，但其他研究结果却相互矛盾。因此，本研究旨在准确、明确地了解雷洛昔芬对绝经后妇女的载脂蛋白 AI（Apo-AI）、载脂蛋白 B（APoB）、脂蛋白（a）（Lp（a））、收缩压（SBP）和舒张压（DBP）的影响：利用科学数据库（包括 PubMed、Scopus、Embase、Web of Science 和 Cochrane 图书馆）进行了系统性文献检索，检索期至 2024 年 5 月。研究质量采用 Cochrane 工具进行评估。随机效应荟萃分析用于汇集结果的标准化均值差异（SMD）和 95% CI：共纳入了 20 项试验，干预时间从 6 周到 144 周不等，共有 2825 名参与者。雷洛昔芬补充剂可显著降低载脂蛋白B（SMD：-0.92；95% CI：-1.49至-0.35；P = 0.001）和脂蛋白（a）（SMD：-0.25；95% CI：-0.39至-0.11；P <0.001），同时提高载脂蛋白AI水平（SMD：0.29；95% CI：0.22至0.36；P <0.001）。相反，对 SBP（WMD：-0.49mmHg；95% CI：-3.01 至 2.04；P = 0.706）和 DBP（WMD：-0.81mmHg；95% CI：-4.04 至 2.41；P = 0.621）未观察到明显影响。此外，亚组分析表明，在干预时间超过12周的研究中，雷洛昔芬能显著降低DBP：这项荟萃分析表明，补充雷洛昔芬可对绝经后妇女的脂蛋白产生有益影响。今后还需要开展研究，探讨雷洛昔芬对绝经后妇女健康状况的影响。

{"title":"The effect of raloxifene supplementation on blood pressure and Apo-lipoproteins in postmenopausal women: A systematic review and meta-analysis","authors":"Nooshin Noshadi , Atefeh Bonyadian , Salehe Zarian , Fatemeh Kazemi , Melika Darzi , Farzaneh Akhavan Tabib , Mahdieh Abbasalizad-Farhangi , Beitullah Alipour , Sarehnaz Aghili","doi":"10.1016/j.prostaglandins.2024.106912","DOIUrl":"10.1016/j.prostaglandins.2024.106912","url":null,"abstract":"<div><h3>Introduction</h3><div>Several studies indicated the ameliorating effects of raloxifene supplementation on apolipoproteins and blood pressure, although others have conflicting findings. Therefore, the present study was conducted in order to accurately and definitively understands the effect of raloxifene on apolipoprotein AI (Apo-AI), apolipoprotein B (APoB), lipoprotein (a) (Lp (a)), systolic blood pressure (SBP) and diastolic blood pressure (DBP) in postmenopausal women.</div></div><div><h3>Methods</h3><div>A systematic literature search was conducted using scientific databases including PubMed, Scopus, Embase, and Web of Science and the Cochrane Library, through May 2024. The quality of studies was assessed using Cochrane tool. Random-effects meta-analysis was used to pool standardized mean differences (SMD) and 95 % CI for the outcomes.</div></div><div><h3>Results</h3><div>Twenty trials, with interventions ranging from 6 to 144 weeks and 2825 participants, were included. Raloxifene supplementation demonstrated significant reductions in ApoB (SMD: −0.92; 95 % CI: −1.49 to −0.35; P = 0.001), and Lp (a) (SMD: −0.25; 95 % CI: −0.39 to −0.11; P < 0.001) while increasing Apo-AI levels (SMD: 0.29; 95 % CI: 0.22–0.36; P < 0.001). Conversely, no significant effects were observed on SBP (WMD: −0.49 mmHg; 95 % CI: −3.01–2.04; P = 0.706), and DBP (WMD: −0.81 mmHg; 95 % CI: −4.04–2.41; P = 0.621). Moreover, subgroup analysis indicated that raloxifene significantly decreased DBP in studies with intervention durations of >12 weeks.</div></div><div><h3>Conclusions</h3><div>This meta-analysis has shown that raloxifene supplementation may have beneficial effects on apolipoproteins in postmenopausal women. Future studies are needed to investigate the effect of raloxifene on health status in in postmenopausal women.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106912"},"PeriodicalIF":2.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential benefit of Lactobacillus acidophilus supplementation to rats fed with a high-fat diet on serum lipid profile, kidney amyloid protein and tumor necrosis factor-alpha level 高脂饮食大鼠补充嗜酸乳杆菌对血清脂质、肾淀粉样蛋白和肿瘤坏死因子-α水平的潜在益处

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-09-23 DOI: 10.1016/j.prostaglandins.2024.106911

Pınar Kar , Gülay Çiftci , Alper Çiftci

Aim

It was aimed to determine the potential effect of Lactobacillus acidophilus supplementation on rats exposed to an experimental high-fat diet on serum lipid profile and kidney total beta amyloid protein (TBAP) and Tumor Necrosis Factor-alpha (TNF-α) levels.

Methods

24 male Sprague-Dawley rats were used in the study to establish 4 groups. Standard rat food (SD) was provided to Group 1 as the control; Group 2 was fed a high-fat diet (HFD); Group 3 consumed SD and received L. acidophilus probiotics; Group 4 was fed HFD and received L. acidophilus probiotics. Body weights were determined weekly during the 12-week trial period. At the end of the experiment, TBP and TNF-α levels in the serum and kidney tissue of the rats were measured by ELISA method. Serum total cholesterol (TC), triglyceride (TG), HDL, LDL, urea and creatinine levels and paraoxanase, amylase and lipase activities were determined by spectrophotometric method on the analyzer device.

Results

When the control (Group 1) group and Group 2 were compared at the end of the experiment, it was found that Group 2 had gained the most weight and that both the blood and kidney tissue levels of TNF-α and TBAP, as well as the quantities of TG, TK, LDL, and urea, were significantly greater (P<0.05). Serum HDL, PON and amylase levels were found to be significantly low (P>0.05). TG, TK, LDL, urea, and the levels of TNF-α and TBAP in serum and renal tissue were shown to be lower in the groups who received L. acidophilus probiotics (Groups 3, 4) when compared to Group 2 (P>0.05). It was observed that HDL, PON and amylase levels increased and approached the control group (P<0.05).

Conclusion

The study's findings showed that probiotic supplementation improved blood levels of TG, TC, HDL, LDL, urea, PON, and amylase as well as serum and kidney tissue levels of TNF-α and TBAP in obese rats fed a high-fat diet.

目的：本研究旨在确定补充嗜酸乳杆菌对暴露于实验性高脂饮食的大鼠血清脂质概况、肾脏总β淀粉样蛋白（TBAP）和肿瘤坏死因子-α（TNF-α）水平的潜在影响。第 1 组以标准大鼠食物（SD）作为对照；第 2 组喂食高脂肪食物（HFD）；第 3 组喂食标准大鼠食物并摄入嗜酸乳杆菌益生菌；第 4 组喂食高脂肪食物并摄入嗜酸乳杆菌益生菌。在为期 12 周的试验期间，每周测定一次体重。实验结束时，采用 ELISA 方法测定大鼠血清和肾组织中的 TBP 和 TNF-α 水平。血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、尿素和肌酐水平，以及副氧杂环戊烷酶、淀粉酶和脂肪酶活性均通过分析仪上的分光光度法进行测定：实验结束后，对比对照组（第1组）和第2组，发现第2组体重增加最多，血液和肾组织中TNF-α和TBAP的含量以及TG、TK、LDL和尿素的含量均显著增加（P0.05）。与第 2 组相比，服用嗜酸乳杆菌益生菌组（第 3、4 组）的 TG、TK、LDL、尿素以及血清和肾组织中 TNF-α 和 TBAP 的含量均较低（P>0.05）。据观察，HDL、PON 和淀粉酶水平有所上升，并接近对照组（PC结论：研究结果表明，补充益生菌可改善以高脂肪饮食喂养的肥胖大鼠血液中的 TG、TC、HDL、LDL、尿素、PON 和淀粉酶水平，以及血清和肾组织中的 TNF-α 和 TBAP 水平。

{"title":"Potential benefit of Lactobacillus acidophilus supplementation to rats fed with a high-fat diet on serum lipid profile, kidney amyloid protein and tumor necrosis factor-alpha level","authors":"Pınar Kar , Gülay Çiftci , Alper Çiftci","doi":"10.1016/j.prostaglandins.2024.106911","DOIUrl":"10.1016/j.prostaglandins.2024.106911","url":null,"abstract":"<div><h3>Aim</h3><div>It was aimed to determine the potential effect of <em>Lactobacillus acidophilus</em> supplementation on rats exposed to an experimental high-fat diet on serum lipid profile and kidney total beta amyloid protein (TBAP) and Tumor Necrosis Factor-alpha (TNF-α) levels.</div></div><div><h3>Methods</h3><div>24 male Sprague-Dawley rats were used in the study to establish 4 groups. Standard rat food (SD) was provided to Group 1 as the control; Group 2 was fed a high-fat diet (HFD); Group 3 consumed SD and received <em>L. acidophilus</em> probiotics; Group 4 was fed HFD and received <em>L. acidophilus</em> probiotics. Body weights were determined weekly during the 12-week trial period. At the end of the experiment, TBP and TNF-α levels in the serum and kidney tissue of the rats were measured by ELISA method. Serum total cholesterol (TC), triglyceride (TG), HDL, LDL, urea and creatinine levels and paraoxanase, amylase and lipase activities were determined by spectrophotometric method on the analyzer device.</div></div><div><h3>Results</h3><div>When the control (Group 1) group and Group 2 were compared at the end of the experiment, it was found that Group 2 had gained the most weight and that both the blood and kidney tissue levels of TNF-α and TBAP, as well as the quantities of TG, TK, LDL, and urea, were significantly greater (P<0.05). Serum HDL, PON and amylase levels were found to be significantly low (P>0.05). TG, TK, LDL, urea, and the levels of TNF-α and TBAP in serum and renal tissue were shown to be lower in the groups who received <em>L. acidophilus</em> probiotics (Groups 3, 4) when compared to Group 2 (P>0.05). It was observed that HDL, PON and amylase levels increased and approached the control group (P<0.05).</div></div><div><h3>Conclusion</h3><div>The study's findings showed that probiotic supplementation improved blood levels of TG, TC, HDL, LDL, urea, PON, and amylase as well as serum and kidney tissue levels of TNF-α and TBAP in obese rats fed a high-fat diet.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106911"},"PeriodicalIF":2.5,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From tradition to science: Possible mechanisms of ghee in supporting bone and joint health 从传统到科学：酥油支持骨骼和关节健康的可能机制。

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-09-21 DOI: 10.1016/j.prostaglandins.2024.106902

Maryam Falahatzadeh , Kianoosh Najafi , Kaveh Bashti

Ghee, a traditional form of clarified butter, has been used for centuries in Ayurvedic medicine for its numerous health benefits. Recent scientific studies have begun to elucidate the molecular mechanisms by which ghee may support bone and joint health. This review explores the bioactive components of ghee, including short-chain fatty acids (SCFAs), medium-chain fatty acids (MCFAs), and fat-soluble vitamins (A, D, E, K2), and their potential therapeutic effects on bone density, joint lubrication, and inflammation. SCFAs in ghee can potentially improve joint lubrication and reduce inflammation. MCFAs and conjugated linoleic acid (CLA) exhibit anti-inflammatory properties, modulating cytokine production and oxidative stress pathways. Vitamins D and K2 in ghee can play potentially crucial roles in calcium metabolism and bone mineralization, while vitamin A supports immune regulation and cartilage health. This review integrates traditional knowledge with contemporary scientific research, highlighting the potential of ghee as a complementary therapy for conditions such as osteoporosis and arthritis. By understanding the molecular mechanisms involved, future studies can focus on this field to shed a light on different effects of ghee on bone and joint health.

酥油是一种传统形式的澄清黄油，几个世纪以来一直被阿育吠陀医学用于治疗各种健康问题。最近的科学研究已开始阐明酥油支持骨骼和关节健康的分子机制。本综述探讨了酥油中的生物活性成分，包括短链脂肪酸（SCFA）、中链脂肪酸（MCFA）和脂溶性维生素（A、D、E、K2），以及它们对骨密度、关节润滑和炎症的潜在治疗作用。酥油中的 SCFA 有可能改善关节润滑和减轻炎症。MCFAs和共轭亚油酸（CLA）具有抗炎特性，可调节细胞因子的产生和氧化应激途径。酥油中的维生素 D 和 K2 可在钙代谢和骨矿化中发挥潜在的关键作用，而维生素 A 则有助于免疫调节和软骨健康。这篇综述将传统知识与当代科学研究相结合，强调了酥油作为骨质疏松症和关节炎等疾病的辅助疗法的潜力。通过了解其中涉及的分子机制，未来的研究可以聚焦这一领域，揭示酥油对骨骼和关节健康的不同影响。

{"title":"From tradition to science: Possible mechanisms of ghee in supporting bone and joint health","authors":"Maryam Falahatzadeh , Kianoosh Najafi , Kaveh Bashti","doi":"10.1016/j.prostaglandins.2024.106902","DOIUrl":"10.1016/j.prostaglandins.2024.106902","url":null,"abstract":"<div><div>Ghee, a traditional form of clarified butter, has been used for centuries in Ayurvedic medicine for its numerous health benefits. Recent scientific studies have begun to elucidate the molecular mechanisms by which ghee may support bone and joint health. This review explores the bioactive components of ghee, including short-chain fatty acids (SCFAs), medium-chain fatty acids (MCFAs), and fat-soluble vitamins (A, D, E, K2), and their potential therapeutic effects on bone density, joint lubrication, and inflammation. SCFAs in ghee can potentially improve joint lubrication and reduce inflammation. MCFAs and conjugated linoleic acid (CLA) exhibit anti-inflammatory properties, modulating cytokine production and oxidative stress pathways. Vitamins D and K2 in ghee can play potentially crucial roles in calcium metabolism and bone mineralization, while vitamin A supports immune regulation and cartilage health. This review integrates traditional knowledge with contemporary scientific research, highlighting the potential of ghee as a complementary therapy for conditions such as osteoporosis and arthritis. By understanding the molecular mechanisms involved, future studies can focus on this field to shed a light on different effects of ghee on bone and joint health.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106902"},"PeriodicalIF":2.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of chia seeds on diabetes, blood pressure, lipid profile, and obesity indicators: Systematic review and meta-regression analysis of 14 randomized controlled trials 奇异籽对糖尿病、血压、血脂和肥胖指标的影响：对 14 项随机对照试验的系统回顾和元回归分析。

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-09-19 DOI: 10.1016/j.prostaglandins.2024.106907

Hawal Lateef Fateh , Dyari H. Ahmed , Maryam Sharifi Najafabadi , Jalal Moludi

This comprehensive systematic review and meta-analysis sought to investigate the impact of chia seed supplementation on obesity indicators and metabolic factors. Through a thorough search of relevant studies up to April 2024, 14 clinical trials involving 835 participants were included in the analysis. The findings revealed a notable decrease in triglyceride levels across both higher and lower doses of chia seeds, with weighted mean differences (WMD) of −8.69 mg/dL and −13.11 mg/dL, respectively. Additionally, a statistically significant reduction in LDL-C levels was observed solely in the higher dosage group, showing a WMD of −4.77 mg/dL. Moreover, although the decrease in systolic blood pressure (-2.78 mmHg) compared to the control group was statistically significant, it was only observed with the higher dosage. These results suggest that chia supplementation may offer beneficial effects on dyslipidemia, hypertension, and body weight, potentially mitigating the risk of cardiovascular disease.

这项全面的系统综述和荟萃分析旨在研究补充奇异籽对肥胖指标和代谢因素的影响。通过对截至 2024 年 4 月的相关研究进行全面搜索，14 项涉及 835 名参与者的临床试验被纳入分析范围。研究结果显示，较高和较低剂量的奇异籽都能显著降低甘油三酯水平，加权平均差（WMD）分别为-8.69毫克/分升和-13.11毫克/分升。此外，只有高剂量组的低密度脂蛋白胆固醇（LDL-C）水平出现了统计学意义上的显著降低，加权平均差（WMD）为-4.77毫克/分升。此外，虽然与对照组相比，收缩压的降低（-2.78mmHg）具有统计学意义，但这只在高剂量组中观察到。这些结果表明，补充奇异果可能会对血脂异常、高血压和体重产生有益的影响，从而有可能降低心血管疾病的风险。

{"title":"The impact of chia seeds on diabetes, blood pressure, lipid profile, and obesity indicators: Systematic review and meta-regression analysis of 14 randomized controlled trials","authors":"Hawal Lateef Fateh , Dyari H. Ahmed , Maryam Sharifi Najafabadi , Jalal Moludi","doi":"10.1016/j.prostaglandins.2024.106907","DOIUrl":"10.1016/j.prostaglandins.2024.106907","url":null,"abstract":"<div><div>This comprehensive systematic review and meta-analysis sought to investigate the impact of chia seed supplementation on obesity indicators and metabolic factors. Through a thorough search of relevant studies up to April 2024, 14 clinical trials involving 835 participants were included in the analysis. The findings revealed a notable decrease in triglyceride levels across both higher and lower doses of chia seeds, with weighted mean differences (WMD) of −8.69 mg/dL and −13.11 mg/dL, respectively. Additionally, a statistically significant reduction in LDL-C levels was observed solely in the higher dosage group, showing a WMD of −4.77 mg/dL. Moreover, although the decrease in systolic blood pressure (-2.78 mmHg) compared to the control group was statistically significant, it was only observed with the higher dosage. These results suggest that chia supplementation may offer beneficial effects on dyslipidemia, hypertension, and body weight, potentially mitigating the risk of cardiovascular disease.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106907"},"PeriodicalIF":2.5,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yanghe Decoction promotes ferroptosis through PPARγ-dependent autophagy to inhibit the malignant progression of triple-negative breast cancer 洋河煎剂通过PPARγ依赖性自噬促进铁变态反应，抑制三阴性乳腺癌的恶性进展

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators

Pub Date : 2024-09-14 DOI: 10.1016/j.prostaglandins.2024.106909

YangZi Cheng, GuiPing Yu, Chen Du, ZhaoHui Chen, XuFeng Liu

Triple-negative breast cancer (TNBC) is a heterogeneous subtype of breast cancer that displays highly aggressive with poor prognosis. Yanghe Decoction (YHD) has been used in the treatment of breast cancer for many years. We aimed to explore the effects of YHD on the malignant phenotypes of MDA-MB-231 cells and the potential mechanism related to PPARγ, autophagy and ferroptosis. The serum of rat containing different concentrations of YHD were collected to culture MDA-MB-231 cells. Cell viability and proliferation were assessed by the CCK-8 assay and EDU staining. Wound healing- and transwell assays were used to detect the capacities of MDA-MB-231 cell migration and invasion. Additionally, the levels of lipid peroxidation, Fe²⁺ and the expression of ferroptosis-related proteins were evaluated. The expression of PPARγ and autophagy-related proteins was assessed using immunofluorescence staining or western blot assay. Then, the PPARγ inhibitor (GW9662), autophagy inhibitor (3-MA) and autophagy inducer (rapamycin; Rap) were used to further study the potential mechanism of YHD on TNBC. Results indicated that contained-YHD serum significantly decreased the viability, proliferation, migration and invasion of TNBC cells. Moreover, YHD promoted lipid peroxidation level, elevated Fe²⁺ content and downregulated GPX4, SLC7A11 and SLC3A2 expression. Besides, autophagy was induced and PPARγ was upregulated by YHD in MDA-MB-231 cells. Furthermore, GW9662 alleviated the impacts of YHD on autophagy of MDA-MB-231 cells. Rap reversed the effects of GW9662 on lipid peroxidation, ferroptosis, proliferation, migration and invasion of MDA-MB-231 cells. 3-MA had the similar effects to GW9662. Collectively, YHD suppressed the malignant progression of MDA-MB-231 cells by inducing ferroptosis through PPARγ-dependent autophagy.

三阴性乳腺癌（TNBC）是乳腺癌的一种异质性亚型，具有高度侵袭性，预后较差。多年来，洋河煎剂一直被用于治疗乳腺癌。我们的目的是探讨洋河提取物对MDA-MB-231细胞恶性表型的影响，以及与PPARγ、自噬和铁变态相关的潜在机制。采集含有不同浓度YHD的大鼠血清培养MDA-MB-231细胞。细胞活力和增殖通过 CCK-8 检测法和 EDU 染色法进行评估。伤口愈合和透孔试验用于检测 MDA-MB-231 细胞的迁移和侵袭能力。此外，还评估了脂质过氧化、Fe2+ 和铁变态反应相关蛋白的表达水平。PPARγ 和自噬相关蛋白的表达采用免疫荧光染色法或 Western 印迹法进行评估。然后，使用 PPARγ 抑制剂（GW9662）、自噬抑制剂（3-MA）和自噬诱导剂（雷帕霉素；Rap）进一步研究 YHD 对 TNBC 的潜在作用机制。结果表明，含YHD血清可显著降低TNBC细胞的活力、增殖、迁移和侵袭。此外，YHD还能促进脂质过氧化水平，升高Fe2+含量，下调GPX4、SLC7A11和SLC3A2的表达。此外，YHD 还诱导了 MDA-MB-231 细胞的自噬，并上调了 PPARγ。此外，GW9662 可减轻 YHD 对 MDA-MB-231 细胞自噬的影响。Rap 逆转了 GW9662 对 MDA-MB-231 细胞脂质过氧化、铁变态反应、增殖、迁移和侵袭的影响。3-MA 的作用与 GW9662 相似。总之，YHD 通过 PPARγ 依赖性自噬诱导铁变态反应，从而抑制了 MDA-MB-231 细胞的恶性发展。

{"title":"Yanghe Decoction promotes ferroptosis through PPARγ-dependent autophagy to inhibit the malignant progression of triple-negative breast cancer","authors":"YangZi Cheng, GuiPing Yu, Chen Du, ZhaoHui Chen, XuFeng Liu","doi":"10.1016/j.prostaglandins.2024.106909","DOIUrl":"10.1016/j.prostaglandins.2024.106909","url":null,"abstract":"<div><div>Triple-negative breast cancer (TNBC) is a heterogeneous subtype of breast cancer that displays highly aggressive with poor prognosis. Yanghe Decoction (YHD) has been used in the treatment of breast cancer for many years. We aimed to explore the effects of YHD on the malignant phenotypes of MDA-MB-231 cells and the potential mechanism related to PPARγ, autophagy and ferroptosis. The serum of rat containing different concentrations of YHD were collected to culture MDA-MB-231 cells. Cell viability and proliferation were assessed by the CCK-8 assay and EDU staining. Wound healing- and transwell assays were used to detect the capacities of MDA-MB-231 cell migration and invasion. Additionally, the levels of lipid peroxidation, Fe<sup>2+</sup> and the expression of ferroptosis-related proteins were evaluated. The expression of PPARγ and autophagy-related proteins was assessed using immunofluorescence staining or western blot assay. Then, the PPARγ inhibitor (GW9662), autophagy inhibitor (3-MA) and autophagy inducer (rapamycin; Rap) were used to further study the potential mechanism of YHD on TNBC. Results indicated that contained-YHD serum significantly decreased the viability, proliferation, migration and invasion of TNBC cells. Moreover, YHD promoted lipid peroxidation level, elevated Fe<sup>2+</sup> content and downregulated GPX4, SLC7A11 and SLC3A2 expression. Besides, autophagy was induced and PPARγ was upregulated by YHD in MDA-MB-231 cells. Furthermore, GW9662 alleviated the impacts of YHD on autophagy of MDA-MB-231 cells. Rap reversed the effects of GW9662 on lipid peroxidation, ferroptosis, proliferation, migration and invasion of MDA-MB-231 cells. 3-MA had the similar effects to GW9662. Collectively, YHD suppressed the malignant progression of MDA-MB-231 cells by inducing ferroptosis through PPARγ-dependent autophagy.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"175 ","pages":"Article 106909"},"PeriodicalIF":2.5,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0