Pub Date : 1999-04-01DOI: 10.1590/S0001-37141999000200009
V. Beloti, M. A. F. Barros, J. C. Freitas, L. Nero, Juliana Aparecida de Souza, E. H. W. Santana, B. Franco
ABSTRACT2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration ofmicrobial colonies in solid culture media, being a key component of the dry rehydratablefilm system used for microbiological analysis of food. This dye is colorless in theoxidized form and red when reduced by microorganisms, due to formation of formazan.In this study, TTC was added to Plate Count Agar (PCA) for enumeration ofmicroorganisms in thirty four pasteurized milk samples, with the aim to verify thefrequency of microorganisms that are unable to reduce TTC. Milk samples weredecimally diluted in saline and pour-plated in PCA plus 0.015% TTC. Colonies werecounted after 24h and 48 h of incubation at 35 o C. From a total of 50,574 colonies,19,665 (38.88%) did not reduce TTC in 48h. It was observed that 571 (6.36%) coloniesthat were colorless in 24h became red in 48h. From those that didn™t reduce TTC in48h, 233 were purified and Gram stained. 229 (98.71%) of them were Gram positivecocci and bacilli. The results show that there is a high percentage of microorganismsunable to reduce TTC in pasteurized milk, which cannot be detected by laboratoryprocedures based on the formation of red colonies.Key words: 2,3,5-triphenyltetrazolium chloride, TTC, pasteurized milk, microbial counts
{"title":"Frequency of 2,3,5-triphenyltetrazolium chloride (TTC) non-reducing bacteria in pasteurized milk","authors":"V. Beloti, M. A. F. Barros, J. C. Freitas, L. Nero, Juliana Aparecida de Souza, E. H. W. Santana, B. Franco","doi":"10.1590/S0001-37141999000200009","DOIUrl":"https://doi.org/10.1590/S0001-37141999000200009","url":null,"abstract":"ABSTRACT2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration ofmicrobial colonies in solid culture media, being a key component of the dry rehydratablefilm system used for microbiological analysis of food. This dye is colorless in theoxidized form and red when reduced by microorganisms, due to formation of formazan.In this study, TTC was added to Plate Count Agar (PCA) for enumeration ofmicroorganisms in thirty four pasteurized milk samples, with the aim to verify thefrequency of microorganisms that are unable to reduce TTC. Milk samples weredecimally diluted in saline and pour-plated in PCA plus 0.015% TTC. Colonies werecounted after 24h and 48 h of incubation at 35 o C. From a total of 50,574 colonies,19,665 (38.88%) did not reduce TTC in 48h. It was observed that 571 (6.36%) coloniesthat were colorless in 24h became red in 48h. From those that didn™t reduce TTC in48h, 233 were purified and Gram stained. 229 (98.71%) of them were Gram positivecocci and bacilli. The results show that there is a high percentage of microorganismsunable to reduce TTC in pasteurized milk, which cannot be detected by laboratoryprocedures based on the formation of red colonies.Key words: 2,3,5-triphenyltetrazolium chloride, TTC, pasteurized milk, microbial counts","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"8 1","pages":"137-140"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90718370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-04-01DOI: 10.1590/S0001-37141999000200008
I. Moreno, A. Lerayer, M. Leitão
One hundred sixty seven strains of Lactococcus lactis were screened for bacteriocin production by well diffusion assay of GM17 agar. Fourteen (8.4%) produced antimicrobial activity other than organic acids, bacteriophages or hydrogen peroxide. The frequency of bacteriocin production ranged from 2% in L. lactis subsp. cremoris up to 12% in L. lactis subsp. lactis. Antimicrobial activities were not observed in any strain of L. lactis subsp. lactis var. diacetylactis. Among thirteen bacteriocin-producing strains and two nisin-producing strains (L. lactis subsp. lactis ATCC 11454 and L. lactis subsp. lactis CNRZ 150), eight (53%) were characterized as lactose-positive (Lac+) and proteinase-negative (Prt-). The bacteriocin-producing cultures were also characterized on the basis of plasmid content. All strains had 2 to 7 plasmids with molecular weights varying from 0.5 to 28.1 Mdal. Four strains (ITAL 435, ITAL 436, ITAL 437 and ITAL 438) showed identical profiles and the other were quite distinct.
{"title":"Detection and characterization of bacteriocin-producing Lactococcus lactis strains","authors":"I. Moreno, A. Lerayer, M. Leitão","doi":"10.1590/S0001-37141999000200008","DOIUrl":"https://doi.org/10.1590/S0001-37141999000200008","url":null,"abstract":"One hundred sixty seven strains of Lactococcus lactis were screened for bacteriocin production by well diffusion assay of GM17 agar. Fourteen (8.4%) produced antimicrobial activity other than organic acids, bacteriophages or hydrogen peroxide. The frequency of bacteriocin production ranged from 2% in L. lactis subsp. cremoris up to 12% in L. lactis subsp. lactis. Antimicrobial activities were not observed in any strain of L. lactis subsp. lactis var. diacetylactis. Among thirteen bacteriocin-producing strains and two nisin-producing strains (L. lactis subsp. lactis ATCC 11454 and L. lactis subsp. lactis CNRZ 150), eight (53%) were characterized as lactose-positive (Lac+) and proteinase-negative (Prt-). The bacteriocin-producing cultures were also characterized on the basis of plasmid content. All strains had 2 to 7 plasmids with molecular weights varying from 0.5 to 28.1 Mdal. Four strains (ITAL 435, ITAL 436, ITAL 437 and ITAL 438) showed identical profiles and the other were quite distinct.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"17 3 1","pages":"130-136"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83215225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-04-01DOI: 10.1590/S0001-37141999000200005
C. H. C. Silva, J. Puls, M. V. Sousa, E. Filho
Uma enzima xilanolitica (xilanase II) foi purificada a partir de culturas de estado solido de Aspergillus fumigatus Fresenius. O peso molecular de xilanase II foi estimado em 19 e 8,5 kDa por SDS-PAGE e FPLC, respectivamente. A enzima purificada apresentou maior atividade a 55°C e pH 5,5, alem de hidrolisar especificamente xilana. Os valores aparentes de Km e Vmax de xilanas soluveis e insoluveis, isoladas de cereal e madeira, mostrou que xilanase IIa foi mais ativa em xilana soluvel de madeira. Estudos sobre produtos de hidrolise de xilanas e xilooligomeros por xilanase II em HPLC revelou que a enzima liberou uma variedade de xilooligomeros (xilobiose-xilohexose) e uma pequena quantidade de xilose a partir de xilooligomeros, apresentando atividade de transferase.
{"title":"Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus Fresenius","authors":"C. H. C. Silva, J. Puls, M. V. Sousa, E. Filho","doi":"10.1590/S0001-37141999000200005","DOIUrl":"https://doi.org/10.1590/S0001-37141999000200005","url":null,"abstract":"Uma enzima xilanolitica (xilanase II) foi purificada a partir de culturas de estado solido de Aspergillus fumigatus Fresenius. O peso molecular de xilanase II foi estimado em 19 e 8,5 kDa por SDS-PAGE e FPLC, respectivamente. A enzima purificada apresentou maior atividade a 55°C e pH 5,5, alem de hidrolisar especificamente xilana. Os valores aparentes de Km e Vmax de xilanas soluveis e insoluveis, isoladas de cereal e madeira, mostrou que xilanase IIa foi mais ativa em xilana soluvel de madeira. Estudos sobre produtos de hidrolise de xilanas e xilooligomeros por xilanase II em HPLC revelou que a enzima liberou uma variedade de xilooligomeros (xilobiose-xilohexose) e uma pequena quantidade de xilose a partir de xilooligomeros, apresentando atividade de transferase.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"122 1","pages":"114-119"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81360016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-04-01DOI: 10.1590/S0001-37141999000200016
P. Carvalho, Joaquim Gilberto de Oliveira, G. Pastore
A linhagem de Mucor sp LB-54, considerada uma potencial produtora de acido gama-linolenico (GLA), foi selecionada para o estudo de diferentes temperaturas de cultivo em agitador rotativo. A linhagem usada neste experimento era capaz de acumular uma quantidade alta de lipideos intracelulares, 20,73 % do peso seco de biomassa e conteudo de GLA de 15 % dentre os acidos graxos totais de sua constituicao apos 5 dias de incubacao a 28°C. Quando a temperatura de cultivo foi diminuida de 28oC para 12°C, o conteudo de GLA aumentou de 15 para 24% dentre os acidos graxos totais de sua constituicao. Com o objetivo de otimizar as condicoes de cultivo para a producao rapida de biomassa e producao de lipideos contendo conteudo alto de GLA, o fungo foi cultivado em duas combinacoes de temperaturas associadas com a suplementacao de fonte de carbono (glicose). A producao maxima de GLA (74mg/l) pela linhagem de Mucor sp LB-54 foi obtida apos 5 dias de incubacao a 28°C em meio base, seguida da adicao de glicose (7% p/v) no meio de cultura e uma posterior incubacao por mais 3 dias a 12°C. A identidade do GLA foi confirmada pelo sistema acoplado cromatografo a gas - espectrometro de massa.
{"title":"Enhancement of gamma-linolenic acid production by the fungus Mucor sp LB-54 by growth temperature","authors":"P. Carvalho, Joaquim Gilberto de Oliveira, G. Pastore","doi":"10.1590/S0001-37141999000200016","DOIUrl":"https://doi.org/10.1590/S0001-37141999000200016","url":null,"abstract":"A linhagem de Mucor sp LB-54, considerada uma potencial produtora de acido gama-linolenico (GLA), foi selecionada para o estudo de diferentes temperaturas de cultivo em agitador rotativo. A linhagem usada neste experimento era capaz de acumular uma quantidade alta de lipideos intracelulares, 20,73 % do peso seco de biomassa e conteudo de GLA de 15 % dentre os acidos graxos totais de sua constituicao apos 5 dias de incubacao a 28°C. Quando a temperatura de cultivo foi diminuida de 28oC para 12°C, o conteudo de GLA aumentou de 15 para 24% dentre os acidos graxos totais de sua constituicao. Com o objetivo de otimizar as condicoes de cultivo para a producao rapida de biomassa e producao de lipideos contendo conteudo alto de GLA, o fungo foi cultivado em duas combinacoes de temperaturas associadas com a suplementacao de fonte de carbono (glicose). A producao maxima de GLA (74mg/l) pela linhagem de Mucor sp LB-54 foi obtida apos 5 dias de incubacao a 28°C em meio base, seguida da adicao de glicose (7% p/v) no meio de cultura e uma posterior incubacao por mais 3 dias a 12°C. A identidade do GLA foi confirmada pelo sistema acoplado cromatografo a gas - espectrometro de massa.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"11 1","pages":"170-175"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86475225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-04-01DOI: 10.1590/S0001-37141999000200007
L. A. S. Miranda, E. Sant’Anna, A. C. Porto
Comparative studies on the growth of Micrococcus varians were carried out in BHI culture medium (control) as well as in a culture medium with 2% diluted sugar cane blackstrap molasses, enriched with 0.1% yeast extract. The experiment was conducted with three samples of the experimental and control media in a 5 liter fermentor with working volume of 3.5 liters, continuous agitation (150 rpm), 35 ± 0.1°C temperature, 0.7 L air. l-1 medium. min -1, initial pH 7.0 ± 0.2, 24 hour fermentation period, and approximate inoculum of 6.0 log10 CFU/ml. Samples were collected at 2-hour intervals. Micrococcus varians grew in the two culture media studied, which confirms the experimental medium viability for the growth of this species. The final average concentration of biomass was higher in the control medium than in the experimental medium: 0.99 g.l-1 and 0.78 g.l-1, respectively. The final number of viable cells at the end of fermentation was 20.65 log10 CFU/ml for the control medium (BHI), while in the experimental medium the number of viable cells was 19.43 log10 CFU/ml. The consumption of total sugars was higher for the biomass in the control medium (79.78%), while only 50.53% was consumed for the experimental medium.
{"title":"The growth of Micrococcus varians by utilizing sugar cane blackstrap molasses as substrate","authors":"L. A. S. Miranda, E. Sant’Anna, A. C. Porto","doi":"10.1590/S0001-37141999000200007","DOIUrl":"https://doi.org/10.1590/S0001-37141999000200007","url":null,"abstract":"Comparative studies on the growth of Micrococcus varians were carried out in BHI culture medium (control) as well as in a culture medium with 2% diluted sugar cane blackstrap molasses, enriched with 0.1% yeast extract. The experiment was conducted with three samples of the experimental and control media in a 5 liter fermentor with working volume of 3.5 liters, continuous agitation (150 rpm), 35 ± 0.1°C temperature, 0.7 L air. l-1 medium. min -1, initial pH 7.0 ± 0.2, 24 hour fermentation period, and approximate inoculum of 6.0 log10 CFU/ml. Samples were collected at 2-hour intervals. Micrococcus varians grew in the two culture media studied, which confirms the experimental medium viability for the growth of this species. The final average concentration of biomass was higher in the control medium than in the experimental medium: 0.99 g.l-1 and 0.78 g.l-1, respectively. The final number of viable cells at the end of fermentation was 20.65 log10 CFU/ml for the control medium (BHI), while in the experimental medium the number of viable cells was 19.43 log10 CFU/ml. The consumption of total sugars was higher for the biomass in the control medium (79.78%), while only 50.53% was consumed for the experimental medium.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"25 1","pages":"125-129"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78185718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-04-01DOI: 10.1590/S0001-37141999000200014
F. Moreira, F. A. D. Lima, S. R. Pedrinho, V. Lenartovicz, C. G. M. Souza, R. Peralta
A strain of Aspergillus tamarii, a filamentous fungus isolated from soil, was able to produce both a-amylase and glucoamylase activities in mineral media supplemented with 1% (w/v) starch or maltose as the carbon source. Static cultivation led to significantly higher yields than those obtained using shaking culture. The production of amylases was tolerant to a wide range of initial culture pH values (from 4 to 10) and temperature (from 25 to 42oC). Two amylases, one a-amylase and one glucoamylase, were separated by ion exchange chromatography. Both partially purified enzymes had optimal activities at pH values between 4.5 and 6.0 and were stable under acid conditions (pH 4.0-7.0). The enzymes exhibited optimal activities at temperatures between 50o and 60o C and were stable for more than ten hours at 55oC.
{"title":"Production of amylases by Aspergillus tamarii","authors":"F. Moreira, F. A. D. Lima, S. R. Pedrinho, V. Lenartovicz, C. G. M. Souza, R. Peralta","doi":"10.1590/S0001-37141999000200014","DOIUrl":"https://doi.org/10.1590/S0001-37141999000200014","url":null,"abstract":"A strain of Aspergillus tamarii, a filamentous fungus isolated from soil, was able to produce both a-amylase and glucoamylase activities in mineral media supplemented with 1% (w/v) starch or maltose as the carbon source. Static cultivation led to significantly higher yields than those obtained using shaking culture. The production of amylases was tolerant to a wide range of initial culture pH values (from 4 to 10) and temperature (from 25 to 42oC). Two amylases, one a-amylase and one glucoamylase, were separated by ion exchange chromatography. Both partially purified enzymes had optimal activities at pH values between 4.5 and 6.0 and were stable under acid conditions (pH 4.0-7.0). The enzymes exhibited optimal activities at temperatures between 50o and 60o C and were stable for more than ten hours at 55oC.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"16 1","pages":"157-162"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73920420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-01-01DOI: 10.1590/S0001-37141999000100007
E. T. González, E. Bonzo, M. G. Echeverría, M. Licursi, M. E. Etcheverrigaray
Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.
{"title":"Enzootic bovine Leukosis: development of an indirect enzyme linked immunosorbent assay (I-Elisa) in seroepidemiological studies","authors":"E. T. González, E. Bonzo, M. G. Echeverría, M. Licursi, M. E. Etcheverrigaray","doi":"10.1590/S0001-37141999000100007","DOIUrl":"https://doi.org/10.1590/S0001-37141999000100007","url":null,"abstract":"Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the establishment of a cut-off level corresponding to 3 times the standard deviation (SD) above the mean for the negative control set of sera provided acceptable specificity, reducing the risk of false positives results. A comparison of the results obtained by AGID test and I-ELISA showed that considering a total of 465 serum samples, all of the 234 samples (50%) that were positive by AGID were positive to the I-ELISA. Of 225 serum samples which yielded negative results in the AGID test, 69 (15%) were found to be positive by the I-ELISA and 156 (33%) were negative by both techniques. Few sera (2%) that were non-specific by AGID were defined as negative or positive by I-ELISA.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"25 1","pages":"37-42"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87139848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-01-01DOI: 10.1590/S0001-37141999000400015
I. C. Simoni, M. J. B. Fernandes, R. M. Custódio, A. Madeira, C. Arns
The susceptibility of the five cell lines - IB-RS-2, RK-13, Vero, BHK-21, CER - to reovirus S1133 and infectious bursal disease virus (IBDV vaccine GBV-8 strain) was studied to better define satisfactory and sensitive cell culture systems. Cultures were compared for presence of CPE, virus titers and detection of viral RNA. CPE and viral RNA were detected in CER and BHK-21 cells after reovirus inoculation and in RK-13 cell line after IBDV inoculation and with high virus titers. Virus replication by production of low virus titers occurred in IB-RS-2 and Vero cells with reovirus and in BHK-21 cell line with IBDV.
{"title":"Susceptibility of cell lines to avian viruses","authors":"I. C. Simoni, M. J. B. Fernandes, R. M. Custódio, A. Madeira, C. Arns","doi":"10.1590/S0001-37141999000400015","DOIUrl":"https://doi.org/10.1590/S0001-37141999000400015","url":null,"abstract":"The susceptibility of the five cell lines - IB-RS-2, RK-13, Vero, BHK-21, CER - to reovirus S1133 and infectious bursal disease virus (IBDV vaccine GBV-8 strain) was studied to better define satisfactory and sensitive cell culture systems. Cultures were compared for presence of CPE, virus titers and detection of viral RNA. CPE and viral RNA were detected in CER and BHK-21 cells after reovirus inoculation and in RK-13 cell line after IBDV inoculation and with high virus titers. Virus replication by production of low virus titers occurred in IB-RS-2 and Vero cells with reovirus and in BHK-21 cell line with IBDV.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"3 1","pages":"373-376"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90234429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-01-01DOI: 10.1590/S0001-37141999000100006
E. R. Nascimento, A. J. Damassa, R. Yamamoto, M. Nascimento
One-hundred-five (105) clinical isolates of mycoplasma from caprine origin and one isolate from ovine were surveyed for plasmids, which were present in thirty-three (31%) of them. These mycoplasmas originated from 13 herds. Ten of them were symptomatic for mycoplasmal disease (mastitis, polyarthritis, septicemia) and three herds were asymptomatic, i.e., clinically normal. Twenty-eight isolates were Mycoplasma mycoides subspecies mycoides LC (large colony or caprine biotype), four were Mycoplasma capricolum subsp. capricolum and one was Mycoplasma cottewii. The isolated plasmids were linearized by EcoRI, EcoRV, EcoRI and EcoRV or BamHI and EcoRV, and were of five sizes (1.1, 1.6, 1.7, 1.8, and 1.9 Kbp). Based on restriction enzyme digestion and size of the linearized supercoiled extrachromosomal DNA, five plasmid types were recovered (p1II, p2III, p2V, p3I, and p4IV). The small size of these DNA elements probably exclude replicative forms of DNA virus, which are equal or larger than 8.0 Kbp.
{"title":"Plasmids in Mycoplasma species isolated from goats and sheep and their preliminary typing","authors":"E. R. Nascimento, A. J. Damassa, R. Yamamoto, M. Nascimento","doi":"10.1590/S0001-37141999000100006","DOIUrl":"https://doi.org/10.1590/S0001-37141999000100006","url":null,"abstract":"One-hundred-five (105) clinical isolates of mycoplasma from caprine origin and one isolate from ovine were surveyed for plasmids, which were present in thirty-three (31%) of them. These mycoplasmas originated from 13 herds. Ten of them were symptomatic for mycoplasmal disease (mastitis, polyarthritis, septicemia) and three herds were asymptomatic, i.e., clinically normal. Twenty-eight isolates were Mycoplasma mycoides subspecies mycoides LC (large colony or caprine biotype), four were Mycoplasma capricolum subsp. capricolum and one was Mycoplasma cottewii. The isolated plasmids were linearized by EcoRI, EcoRV, EcoRI and EcoRV or BamHI and EcoRV, and were of five sizes (1.1, 1.6, 1.7, 1.8, and 1.9 Kbp). Based on restriction enzyme digestion and size of the linearized supercoiled extrachromosomal DNA, five plasmid types were recovered (p1II, p2III, p2V, p3I, and p4IV). The small size of these DNA elements probably exclude replicative forms of DNA virus, which are equal or larger than 8.0 Kbp.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"74 1","pages":"32-36"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79422296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-01-01DOI: 10.1590/S0001-37141999000100004
J. R. Modolo, Luiz Florêncio Fernandes Margato, Arnold Frederico Gottschalk, C. A. Lopes
Two hundred pigs (1- 21 weeks old), from five piggeries in Sao Paulo State, Brazil, were divided in two groups of 100 animals each, G1 with diarrhea and G2 without diarrhea. Campylobacter was recovered from 43% of G1 and 34% of G2 specimens, and was more frequently recovered from 0-4 week old piglets. C. coli was the most common species (44.2% in G1 and 32.4% in G2), followed by C. jejuni/coli (16.3% in G1 and 23.5% in G2). Campylobacter counts were significantly higher in G1 (£ 108 UFC/g) than in G2 (£ 104 UFC/g) (p < 0.01), which suggests that the bacterium may play a role at least in the aggravation of the diarrheic process.
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