Pub Date : 1998-09-01DOI: 10.1590/S0001-37141998000300015
Vera Lúcia Mores Rall, S. T. Iaria, Sandra Heidtmann, Fabiana Cristina Pimenta, R. Gamba, Débora Midori Myaki Pedroso
Bacterias do genero Aeromonas tem sido descritas como patogenos emergentes de importância crescente em alimentos. Neste estudo, relatamos que 48% das amostras de peixe "Pintado" coletado no comercio de Sao Paulo, foram positivas para Aeromonas sp quando isoladas pelo metodo de plaqueamento direto. Quando o metodo Presenca/Ausencia foi utilizado, a porcentagem de positividade foi de 42%. A. caviae foi a especie mais frequente, seguida por A. hydrophila e A. sobria. Producao de enterotoxina citotoxica, determinada em camundongos recem-nascidos, foi observada em 67% das cepas de A. sobria, em 60% das de A. hydrophila e em 40% das de A. caviae. No teste in vitro em celulas HEp- 88% das cepas de A. hydrophila, 27% das cepas de A. sobria e 13% das cepas de A. caviae revelaram-se positivas. Com relacao a producao de enterotoxina citotonica, testada apos o aquecimento do sobrenadante a 56oC por 20 minutos, 17% das cepas de A. sobria, 10% das de A. caviae e nenhuma das de A. hydrophila foram positivas in vivo e para todas as cepas analisadas, os testes foram negativos em cultura de celula HEp-2. Quanto a capacidade de adesao, 20% das 5 cepas de A. sobria e 16% das 20 cepas de A. caviae aderiram a celulas HEp-2. A capacidade de invasao em celulas HEp-2 nao foi detectada em nenhuma das cepas testadas. As cepas isoladas foram sensiveis a maior parte dos antimicrobianos testados.
{"title":"Aeromonas species isolated from PINTADO fish (Pseudoplatystoma sp): virulence factors and drug susceptibility","authors":"Vera Lúcia Mores Rall, S. T. Iaria, Sandra Heidtmann, Fabiana Cristina Pimenta, R. Gamba, Débora Midori Myaki Pedroso","doi":"10.1590/S0001-37141998000300015","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300015","url":null,"abstract":"Bacterias do genero Aeromonas tem sido descritas como patogenos emergentes de importância crescente em alimentos. Neste estudo, relatamos que 48% das amostras de peixe \"Pintado\" coletado no comercio de Sao Paulo, foram positivas para Aeromonas sp quando isoladas pelo metodo de plaqueamento direto. Quando o metodo Presenca/Ausencia foi utilizado, a porcentagem de positividade foi de 42%. A. caviae foi a especie mais frequente, seguida por A. hydrophila e A. sobria. Producao de enterotoxina citotoxica, determinada em camundongos recem-nascidos, foi observada em 67% das cepas de A. sobria, em 60% das de A. hydrophila e em 40% das de A. caviae. No teste in vitro em celulas HEp- 88% das cepas de A. hydrophila, 27% das cepas de A. sobria e 13% das cepas de A. caviae revelaram-se positivas. Com relacao a producao de enterotoxina citotonica, testada apos o aquecimento do sobrenadante a 56oC por 20 minutos, 17% das cepas de A. sobria, 10% das de A. caviae e nenhuma das de A. hydrophila foram positivas in vivo e para todas as cepas analisadas, os testes foram negativos em cultura de celula HEp-2. Quanto a capacidade de adesao, 20% das 5 cepas de A. sobria e 16% das 20 cepas de A. caviae aderiram a celulas HEp-2. A capacidade de invasao em celulas HEp-2 nao foi detectada em nenhuma das cepas testadas. As cepas isoladas foram sensiveis a maior parte dos antimicrobianos testados.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"33 1","pages":"222-227"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87959965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-09-01DOI: 10.1590/S0001-37141998000300009
I. Lara-Herrera, G. Mata, R. Gaitán-Hernández
Viability of 6 mushroom strains of the Pleurotus genus (2 from P. djamor var. djamor, 1 from P. ostreatus var. ostreatus, 2 from P. ostreatus var. columbinus and 1 from P. pulmonarius) after liquid nitrogen cryopreservation (-196o) was evaluated. The contact time for the mycelia of these strains with the cryoprotectant (glycerol) was studied 1, 2 and 3 hours before freezing. We also tested the effect of different times (5, 10 and 15 minutes) and temperatures (30, 45 and 60oC) of the thawing system for mycelial recovery. The results showed a marked tendency toward faster mycelial recovery when samples were thawed at 30oC, while at 60oC no recovery was observed. A change in thawing and contact times with the cryoprotectant did not affect the results significantly, as the thawing temperature and strain employed affected.
对6株侧耳菇属菌种(2株为大叶扁菇，1株为ostreatus var. ostreatus, 2株为ostreatus var. columbinus, 1株为pulmonarius)在液氮低温保存(- 1960)后的生存能力进行了评价。在冷冻前1、2和3小时，研究了这些菌株的菌丝与冷冻保护剂(甘油)的接触时间。我们还测试了不同解冻时间(5、10和15分钟)和温度(30、45和60℃)对菌丝恢复的影响。结果表明，当样品在30℃解冻时，菌丝恢复速度明显加快，而在60℃解冻时则没有恢复。解冻和接触冷冻保护剂的时间的变化不会显著影响结果，因为解冻温度和所用的应变会影响结果。
{"title":"EVALUATION OF THE VIABILITY OF PLEUROTUS SPP. STRAINS AFTER LIQUID NITROGEN CRYOPRESERVATION","authors":"I. Lara-Herrera, G. Mata, R. Gaitán-Hernández","doi":"10.1590/S0001-37141998000300009","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300009","url":null,"abstract":"Viability of 6 mushroom strains of the Pleurotus genus (2 from P. djamor var. djamor, 1 from P. ostreatus var. ostreatus, 2 from P. ostreatus var. columbinus and 1 from P. pulmonarius) after liquid nitrogen cryopreservation (-196o) was evaluated. The contact time for the mycelia of these strains with the cryoprotectant (glycerol) was studied 1, 2 and 3 hours before freezing. We also tested the effect of different times (5, 10 and 15 minutes) and temperatures (30, 45 and 60oC) of the thawing system for mycelial recovery. The results showed a marked tendency toward faster mycelial recovery when samples were thawed at 30oC, while at 60oC no recovery was observed. A change in thawing and contact times with the cryoprotectant did not affect the results significantly, as the thawing temperature and strain employed affected.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"44 1","pages":"193-196"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85926019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-09-01DOI: 10.1590/S0001-37141998000300012
A. Bramorski, C. R. Soccol, P. Christen, S. Revah
Solid state fermentations were carried out to test the efficacy of Ceratocystis fimbriata to grow on different agro-industrial substrates and aroma production. Seven media were prepared using cassava bagasse, apple pomace, amaranth and soya bean. All the media supported fungal growth. While amaranth medium produced pineapple aroma, media containing cassava bagasse, apple pomace and soya bean produced a strong fruity aroma. The aroma production was growth dependent and the maximum aroma intensity was detected a few hours before or after the maximum respirometric activity. Sixteen compounds were separated by gas cromatography of the components present in the headspace and fifteen of them were identified as acid (1), alcohols (6), aldehyde (1), ketones (2) and esters (5).
{"title":"FRUITY AROMA PRODUCTION BY Ceratocystis fimbriata IN SOLID CULTURES FROM AGRO-INDUSTRIAL WASTES","authors":"A. Bramorski, C. R. Soccol, P. Christen, S. Revah","doi":"10.1590/S0001-37141998000300012","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300012","url":null,"abstract":"Solid state fermentations were carried out to test the efficacy of Ceratocystis fimbriata to grow on different agro-industrial substrates and aroma production. Seven media were prepared using cassava bagasse, apple pomace, amaranth and soya bean. All the media supported fungal growth. While amaranth medium produced pineapple aroma, media containing cassava bagasse, apple pomace and soya bean produced a strong fruity aroma. The aroma production was growth dependent and the maximum aroma intensity was detected a few hours before or after the maximum respirometric activity. Sixteen compounds were separated by gas cromatography of the components present in the headspace and fifteen of them were identified as acid (1), alcohols (6), aldehyde (1), ketones (2) and esters (5).","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"60 1","pages":"208-212"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84469573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-09-01DOI: 10.1590/S0001-37141998000300011
E. Sant’Anna, R. C. Torres
Pediococcus acidilactici (IL01) has grown in MRS (Man, Rogosa and Sharpe) broth modified by substitution of glucose by 2.0% (MRS-2), 3.0% (MRS-3), 4.0% (MRS-4) and 5.0% (MRS-5) sugar cane blackstrap molasses. The highest acid production was obtained in MRS-5 broth maintained at a constant pH of 5.0. The highest biomass production was obtained when P. acidilactici was grown in MRS-5 broth at initial pH 6.5, while productivity was higher in MRS-2 broth (28.16%). When the MRS-2 broth was utilized at initial pH 6.5 for a 20-hour fermentation period, the highest growth rate (dx/dt) was found in a period of 8 to 16 hours (0.290 g cells/L.h), while the specific growth rate (µ) was 0.175 (h-1) for that period, differently from the 0.441 (h-1) obtained for the period comprising the 4th to the 12th hour. The growth in MRS broth was 5.08% (2.95 g/l) higher than in MRS-2 broth (2.80 g/l). The data obtained have shown that P. acidilactici has had a significant growth in molasses as the main carbon source, and that it is possible to substitute MRS glucose by this carbon source with the purpose of obtaining a more economical growth medium for the potential large scale productions.
{"title":"Growth of Pediococcus acidilactici on sugar cane blackstrap molasses","authors":"E. Sant’Anna, R. C. Torres","doi":"10.1590/S0001-37141998000300011","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300011","url":null,"abstract":"Pediococcus acidilactici (IL01) has grown in MRS (Man, Rogosa and Sharpe) broth modified by substitution of glucose by 2.0% (MRS-2), 3.0% (MRS-3), 4.0% (MRS-4) and 5.0% (MRS-5) sugar cane blackstrap molasses. The highest acid production was obtained in MRS-5 broth maintained at a constant pH of 5.0. The highest biomass production was obtained when P. acidilactici was grown in MRS-5 broth at initial pH 6.5, while productivity was higher in MRS-2 broth (28.16%). When the MRS-2 broth was utilized at initial pH 6.5 for a 20-hour fermentation period, the highest growth rate (dx/dt) was found in a period of 8 to 16 hours (0.290 g cells/L.h), while the specific growth rate (µ) was 0.175 (h-1) for that period, differently from the 0.441 (h-1) obtained for the period comprising the 4th to the 12th hour. The growth in MRS broth was 5.08% (2.95 g/l) higher than in MRS-2 broth (2.80 g/l). The data obtained have shown that P. acidilactici has had a significant growth in molasses as the main carbon source, and that it is possible to substitute MRS glucose by this carbon source with the purpose of obtaining a more economical growth medium for the potential large scale productions.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"26 1","pages":"202-207"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82689073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-07-01DOI: 10.1590/S0001-37141998000300008
E. Gomes, R. Silva, A. Serzedello
Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Lab Bioquim Proc & Microbiol Aplicada, BR-15054000 Sao Jose Dos Campos, Brazil
保利斯塔州立大学，生物科学学院，生物quim Proc和应用微生物实验室，BR-15054000 Sao Jose Dos Campos，巴西
{"title":"Ribonuclease Production by Aspergillus species","authors":"E. Gomes, R. Silva, A. Serzedello","doi":"10.1590/S0001-37141998000300008","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300008","url":null,"abstract":"Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Lab Bioquim Proc & Microbiol Aplicada, BR-15054000 Sao Jose Dos Campos, Brazil","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"46 1","pages":"187-192"},"PeriodicalIF":0.0,"publicationDate":"1998-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84645438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-01-01DOI: 10.1590/S0001-37141998000300005
R. C. Ferreira, A. O. A. Neto, S. Fracalanzza, S. Costa, D. F. Almeida, L. Ferreira
The electrophoretic profiles of penicillin binding proteins (PBPs) and outer membrane proteins (OMPs) of Yersinia pestis EV 76 were determined following in vivo growth in diffusion chambers implanted in the peritoneal cavity of mice. In contrast to Y. pestis grown under in vitro conditions which activate the low calcium response (LCR) regulon there was no significant qualitative or quantitative change of the PBP profile of Y. pestis cells during growth in diffusion chambers for up to 72 h following implantation in mice. Three OMPs, with molecular weight of 100, 60 and 58 kDa, were expressed in Y. pestis cells grown for 24 h, but not at 48 h or at 72 h, in diffusion chambers. These results indicate that growth of Y. pestis in intraperitoneal diffusion chambers activates genes which might be relevant to the growth in the mammal host.
{"title":"Cell envelope components of Yersinia pestis grown in intraperitoneal diffusion chambers","authors":"R. C. Ferreira, A. O. A. Neto, S. Fracalanzza, S. Costa, D. F. Almeida, L. Ferreira","doi":"10.1590/S0001-37141998000300005","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300005","url":null,"abstract":"The electrophoretic profiles of penicillin binding proteins (PBPs) and outer membrane proteins (OMPs) of Yersinia pestis EV 76 were determined following in vivo growth in diffusion chambers implanted in the peritoneal cavity of mice. In contrast to Y. pestis grown under in vitro conditions which activate the low calcium response (LCR) regulon there was no significant qualitative or quantitative change of the PBP profile of Y. pestis cells during growth in diffusion chambers for up to 72 h following implantation in mice. Three OMPs, with molecular weight of 100, 60 and 58 kDa, were expressed in Y. pestis cells grown for 24 h, but not at 48 h or at 72 h, in diffusion chambers. These results indicate that growth of Y. pestis in intraperitoneal diffusion chambers activates genes which might be relevant to the growth in the mammal host.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"96 1","pages":"174-178"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73432611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-01-01DOI: 10.1590/S0001-37141998000300010
S. Freire, L. M. Paiva, E. A. L. Lima, L. Maia
Curvularia pallescens Boedijn (Hyphomycetes) is redescribed with the aid of a scanning electron microscope, and the optimal cultural conditions for growing this fungus are discussed. Cytological analysis and nuclear condition, observed through the HCl-Giemsa technique, showed vegetative and reproductive structures (hypha and conidia) formed by uni, bi, tri, and multinucleated segments. Cultures of C. pallescens in Complete medium and in Potato dextrose agar varied on growth, on aspects of the border of the colonies and also on medium pigmentation. The Complete medium and the temperature between 25-28°C were the most indicated for growth of C. pallescens.
{"title":"MORPHOLOGICAL, CYTOLOGICAL, AND CULTURAL ASPECTS OF CURVULARIA PALLESCENS","authors":"S. Freire, L. M. Paiva, E. A. L. Lima, L. Maia","doi":"10.1590/S0001-37141998000300010","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300010","url":null,"abstract":"Curvularia pallescens Boedijn (Hyphomycetes) is redescribed with the aid of a scanning electron microscope, and the optimal cultural conditions for growing this fungus are discussed. Cytological analysis and nuclear condition, observed through the HCl-Giemsa technique, showed vegetative and reproductive structures (hypha and conidia) formed by uni, bi, tri, and multinucleated segments. Cultures of C. pallescens in Complete medium and in Potato dextrose agar varied on growth, on aspects of the border of the colonies and also on medium pigmentation. The Complete medium and the temperature between 25-28°C were the most indicated for growth of C. pallescens.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"2004 1","pages":"197-201"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82568911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-01-01DOI: 10.11606/d.11.2018.tde-20181127-161433
P. C. Vertoni, R. Camargo
{"title":"Producao de enterotoxinas por linhagens de staphylococcus aureus rosenbach, 1984, isoladas de fossas nasais e de algumas amostras clinicas","authors":"P. C. Vertoni, R. Camargo","doi":"10.11606/d.11.2018.tde-20181127-161433","DOIUrl":"https://doi.org/10.11606/d.11.2018.tde-20181127-161433","url":null,"abstract":"","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"91 1","pages":"172"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77627591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-11-29DOI: 10.11606/D.11.2019.TDE-20191218-110710
E. Gomes, H. Amorim
{"title":"Efeito do tratamento acido da levedura saccharomyces cerevisiae na fermentacao alcoolica","authors":"E. Gomes, H. Amorim","doi":"10.11606/D.11.2019.TDE-20191218-110710","DOIUrl":"https://doi.org/10.11606/D.11.2019.TDE-20191218-110710","url":null,"abstract":"","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"53 1","pages":"31"},"PeriodicalIF":0.0,"publicationDate":"1988-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72820007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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