Revista Iberoamericana De Micologia最新文献

英文中文

Pneumocystis in dogs: A protozoan knockin’ on Fungi's door 狗的肺囊虫:敲开真菌大门的原生动物

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-10-01 DOI: 10.1016/j.riam.2021.04.001

Francisco Javier Cabañes

引用次数: 0

Filamentous fungi in the airway of patients with cystic fibrosis: Just spectators? 囊性纤维化患者气道中的丝状真菌:只是旁观者?

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-10-01 DOI: 10.1016/j.riam.2021.03.005

Clara Viñado , Rosa María Girón , Elisa Ibáñez , Alberto García-Ortega , Inés Pérez , Dinora Polanco , Javier Pemán , Amparo Solé

Background

There are important advances in the management of bacterial infection in patients with cystic fibrosis (CF), but there are many gaps in the field of fungal infections.

Aims

The aim of this study was to analyse whether chronic respiratory filamentous fungal colonization had clinical impact and whether antifungal treatment can change the disease.

Methods

The prospective, bicentric and descriptive study was carried out within a 3-year follow-up period, with four-month periodicity medical controls. Adult patients from two CF units of tertiary hospitals were included. Clinical, microbiological, analytical and spirometric variables were collected. Quality of life was evaluated in a subgroup, using the Spanish version of the Revised Cystic Fibrosis Quality of Life Questionnaire (CFQ-R). To statistically analyze the evolution of forced expiratory along time (volume of air blown out in 1 second -FEV₁-) and the forced vital capacity (FVC), mixed linear models were carried out.

Results

From the ninety-eight patients under study, 40 suffered chronic filamentous fungal colonization. The presence of filamentous fungi in airway was associated to an annual fall of FEV₁ and FVC of 0.029 and 0.017 litres, respectively (p<0.001). In addition, worse quality of life based on CFQ-R, significant when concerning physical condition and emotional state, was also linked with the fungal colonization. Protocolized antifungal therapy, nebulized or oral, improved FEV₁ in 0.023 and 0.024 litres per year, respectively (p<0.001).

Conclusions

Chronic filamentous fungal colonization in patients with CF is associated with a significant annual decline of lung function that persists over time. Chronic antifungal therapy slows down this progression, mainly in the patient with more advanced disease.

背景在囊性纤维化(CF)患者细菌感染的管理方面取得了重要进展，但真菌感染领域仍有许多空白。目的分析慢性呼吸道丝状真菌定植是否有临床影响以及抗真菌治疗是否可以改变疾病。方法采用前瞻性、双中心、描述性研究，随访3年，定期医学对照4个月。纳入了来自两家三级医院CF病房的成年患者。收集临床、微生物学、分析学和肺活量学变量。使用西班牙语版囊性纤维化生活质量问卷(CFQ-R)对一个亚组的生活质量进行评估。为了统计用力呼气随时间(1秒内呼出气量- fev1 -)和用力肺活量(FVC)的变化，采用混合线性模型。结果98例患者中，40例为慢性丝状真菌定植。气道中丝状真菌的存在与FEV1和FVC每年分别下降0.029和0.017升有关(p<0.001)。此外，基于CFQ-R的较差的生活质量(在身体状况和情绪状态方面显著)也与真菌定植有关。方案抗真菌治疗，雾化或口服，每年分别改善0.023和0.024升FEV1 (p<0.001)。结论慢性丝状真菌定植与CF患者肺功能的逐年显著下降有关，并持续一段时间。慢性抗真菌治疗减缓了这种进展，主要是在病情较晚期的患者中。

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引用次数: 0

Invasive scedosporiosis in lung transplant recipients: A nine-year retrospective study in a tertiary care hospital 肺移植受者侵袭性孢子虫病:一项三级医院9年回顾性研究

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-10-01 DOI: 10.1016/j.riam.2021.06.002

Elisa Ibáñez-Martínez , Amparo Solé , Antonio Cañada-Martínez , Carlos F. Muñoz-Núñez , Amparo Pastor , Beatriz Montull , Patricia Falomir-Salcedo , Amparo Valentín , José Luis López-Hontangas , Javier Pemán

Background

Scedosporium species and Lomentospora prolificans (Sc/Lp) are emerging molds that cause invasive disease associated with a high mortality rate. After Aspergillus, these molds are the second filamentous fungi recovered in lung transplant (LT) recipients.

Aims

Our objective was to evaluate the incidence, risk factors and outcome of Sc/Lp infections in LT recipients at a tertiary care hospital with a national reference LT program.

Methods

A nine-year retrospective study was conducted.

Results

During this period, 395 LT were performed. Positive cultures for Sc/Lp were obtained from twenty-one LT recipients. Twelve patients (incidence 3.04%) developed invasive scedosporiosis (IS). In 66.7% of the patients with IS the invasive infection was defined as a breakthrough one. The main sites of infection were lungs and paranasal sinuses. Most of the patients received combination antifungal therapy. The IS crude mortality rate after 30 days was 16.7%, and 33.3% after a year.

Conclusions

Our study highlights improved survival rates associated with combination antifungal therapy in LT recipients and underlines the risk of breakthrough infections in patients with allograft dysfunction on nebulized lipidic amphotericin B prophylaxis. In addition to pretransplant colonization, acute or chronic organ dysfunctions seem to be the main risk factors for IS.

背景塞多孢子菌和增毒骆孢菌(Lomentospora prolificans, Sc/Lp)是一种新兴的霉菌，可引起侵袭性疾病，死亡率高。在曲霉之后，这些霉菌是在肺移植(LT)受者中发现的第二种丝状真菌。目的:本研究的目的是评估一家三级医院肝移植受者Sc/Lp感染的发生率、危险因素和结果。方法采用9年回顾性研究。结果本组共行肝移植395例。21例肝移植受体获得Sc/Lp阳性培养。12例(发病率3.04%)发生侵袭性孢子病(IS)。66.7%的IS患者将侵袭性感染定义为突破性感染。主要感染部位为肺部和鼻窦。大多数患者接受联合抗真菌治疗。30天后IS粗死亡率为16.7%，一年后为33.3%。结论:我们的研究强调了肝移植受者联合抗真菌治疗的生存率提高，并强调了雾化脂质两性霉素B预防异体移植物功能障碍患者突破性感染的风险。除了移植前定植外，急性或慢性器官功能障碍似乎是IS的主要危险因素。

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引用次数: 3

Cyberlindnera fabianii: primer aislamiento clínico en Chile fabianii Cyberlindnera:智利首次临床隔离

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-10-01 DOI: 10.1016/j.riam.2021.04.009

Eduardo Álvarez Duarte , Nicolás Cepeda

Background

Although considered an unusual etiological agent, Cyberlindnera (Candida) fabianii has been related to septicemia in several reports in recent years. Its doubtful or uncertain identification when using tests such as CHROMagar Candida, API® Candida, API® ID32C or VITEK® MS, leads to an underestimation of the cases produced by this yeast.

Aims

To report the first isolation of C. fabianii in Chile and its identification.

Methods

The sequencing of the internal transcribed spacer region (ITS) was performed. Antifungal susceptibility profiles were obtained by means of the broth microdilution technique.

Results

The identification was only reached by sequencing the ITS regions, which shows the limited usefulness of the conventional techniques in the identification of some yeast species. A dendrogram shows the phylogenetic relationship of the isolated strain with some other yeast species.

Conclusion

In the identification of fastidious microorganisms or microorganisms whose identification is not completely reliable when using classical or even advanced methodologies, such as mass spectrometry, sequencing techniques are essential.

虽然被认为是一种不寻常的病原，但近年来在一些报道中，fabiani念珠菌与败血症有关。当使用CHROMagar念珠菌，API®念珠菌，API®ID32C或VITEK®MS等测试时，其可疑或不确定的鉴定导致对该酵母产生的病例的低估。目的报道在智利首次分离到的法比亚氏弧菌及其鉴定。方法对其内部转录间隔区(ITS)进行测序。采用肉汤微量稀释法获得了菌株的抗真菌药敏谱。结果仅通过对ITS区域的测序进行鉴定，表明传统技术在某些酵母种类鉴定中的有效性有限。树状图显示分离菌株与其他酵母菌种的系统发育关系。结论在使用经典甚至先进的方法(如质谱法)鉴定难以完全可靠的微生物或精细微生物时，测序技术是必不可少的。

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引用次数: 1

Characterization, enzymatic activity and biofilm formation of Candida species isolated from goat milk 从羊奶中分离的念珠菌的鉴定、酶活性和生物膜形成

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-10-01 DOI: 10.1016/j.riam.2021.06.001

Carolina Segundo Zaragoza , Itzel López Ortiz , David Alejandro Contreras Caro del Castillo , Yesmín María Domínguez Hernández , Juan Antonio Rodríguez García

Background

Data regarding yeast microbiota in goat milk is scarce.

Aims

To isolate and identify species of the genus Candida in milk samples from clinically healthy goats, and evaluate their enzymatic activity and biofilm formation.

Methods

1092 milk samples from clinically healthy goats were collected and processed. The yeast isolates were identified by phenotypic, methods and their enzymatic activity (phospholipase, hemolysin and protease) and biofilm formation evaluated.

Results

We obtained 221 Candida isolates belonging to six species: Candida kefyr (35.7%), Candida guilliermondii (33%), Candida famata (23.5%), Candida glabrata (5.9%), Candida albicans (1.35%) and Candida parapsilosis sensu lato (0.45%). Protease activity was detected in all Candida species while hemolysin activity was only present in C. kefyr, C. guilliermondii, C. famata and C. albicans. Only C. albicans showed phospholipase activity. With the exception of C. parapsilosis sensu lato, all Candida species formed biofilm, with 60.19% of the isolates being poor producers, 9.93% moderate producers, and 1.35% strong producers.

Conclusions

The milk of clinically healthy goats contains several species of the genus Candida that could play a role as opportunistic pathogens in mastitis.

关于羊奶中酵母菌群的数据很少。目的分离鉴定临床健康山羊乳样品中的念珠菌属，并对其酶活性和生物膜形成进行评价。方法采集临床健康山羊羊奶样品1092份，并进行处理。对分离的酵母菌进行表型、方法鉴定，并对其酶活性(磷脂酶、溶血酶和蛋白酶)和生物膜形成进行评价。结果共分离念珠菌221株，分属6种:关键念珠菌(35.7%)、吉列mondii念珠菌(33%)、famata念珠菌(23.5%)、光秃念珠菌(5.9%)、白色念珠菌(1.35%)和敏感副念珠菌(0.45%)。蛋白酶在所有念珠菌中均有活性，溶血素仅在C. kefyr、C. guilliermondii、C. famata和C. albicans中有活性。只有白色念珠菌显示磷脂酶活性。除假丝酵母菌外，所有念珠菌均形成生物膜，60.19%为弱产菌，9.93%为中等产菌，1.35%为强产菌。结论临床健康山羊乳汁中含有多种念珠菌属，可能是乳腺炎的条件致病菌。

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引用次数: 2

Super moulds and Scedosporium species 超级霉菌和孢子种类

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-10-01 DOI: 10.1016/j.riam.2021.05.001

F. Javier Cabañes

引用次数: 0

Differentially expressed proteins in the interaction of Paracoccidioides lutzii with human monocytes 鲁茨副球虫与人单核细胞相互作用中的差异表达蛋白

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-10-01 DOI: 10.1016/j.riam.2020.09.006

Flamélia Carla Silva Oliveira , Wallace Felipe Blohem Pessoa , Joise Hander Mares , Herbert Pina Silva Freire , Ednara Almeida de Souza , Carlos Priminho Pirovani , Carla Cristina Romano

Background

Fungi of the genus Paracoccidioides are the etiological agents of paracoccidioidomycosis, a highly prevalent mycosis in Latin America. Infection in humans occurs by the inhalation of conidia, which later revert to the form of yeast. In this context, macrophages are positioned as an important line of defense, assisting in the recognition and presentation of antigens, as well as producing reactive oxygen species that inhibit fungal spreading.

Aims

The objective of this study was to identify differentially expressed proteins during the interaction between Paracoccidioides lutzii Pb01 strain and human U937 monocytes.

Methods

Two-dimensional electrophoresis, combined with mass spectrometry, was used to evaluate the differential proteomic profiles of the fungus P. lutzii (Pb01) interacting with U937 monocytes.

Results

It was possible to identify 25 proteins differentially expressed by Pb01 alone and after interacting with U937 monocytes. Most of these proteins are directly associated with fungal metabolism for energy generation, such as glyceraldehyde-3-phosphate dehydrogenase, and intracellular adaptation to monocytes. Antioxidant proteins involved in the response to oxidative stress, such as peroxiredoxin, cytochrome, and peroxidase, were expressed in greater quantity in the interaction with monocytes, suggesting their association with survival mechanisms inside phagocytic cells. We also identified 12 proteins differentially expressed in monocytes before and after the interaction with the fungus; proteins involved in the reorganization of the cytoskeleton, such as vimentin, and proteins involved in the response to oxidative stress, such as glioxalase 1, were identified.

Conclusions

The results of this proteomic study of a P. lutzii isolate are novel, mimicking in vitro what occurs in human infections. In addition, the proteins identified may aid to understand fungal–monocyte interactions and the pathogenesis of paracoccidioidomycosis.

副球孢子菌属真菌是副球孢子菌病的病原，是拉丁美洲一种高度流行的真菌病。人类的感染是通过吸入分生孢子发生的，分生孢子后来恢复为酵母菌的形式。在这种情况下，巨噬细胞被定位为重要的防线，协助抗原的识别和呈递，以及产生抑制真菌传播的活性氧。目的研究鲁茨副球虫Pb01菌株与人U937单核细胞相互作用过程中的差异表达蛋白。方法采用双向电泳和质谱联用技术，对与U937单核细胞相互作用的真菌P. lutzii (Pb01)进行差异蛋白质组学分析。结果Pb01单独表达和与U937单核细胞相互作用后可鉴定出25个差异表达蛋白。这些蛋白质中的大多数与真菌产生能量的代谢直接相关，如甘油醛-3-磷酸脱氢酶，以及细胞内对单核细胞的适应。参与氧化应激反应的抗氧化蛋白，如过氧化物还蛋白、细胞色素和过氧化物酶，在与单核细胞的相互作用中表达量更多，表明它们与吞噬细胞内的生存机制有关。我们还鉴定了12种蛋白在与真菌相互作用前后在单核细胞中差异表达;鉴定了参与细胞骨架重组的蛋白质，如vimentin，以及参与氧化应激反应的蛋白质，如glioxalase 1。结论鲁氏疟原虫分离物的蛋白质组学研究结果新颖，在体外模拟了人类感染的情况。此外，鉴定的蛋白质可能有助于了解真菌与单核细胞的相互作用和副球孢子菌病的发病机制。

{"title":"Differentially expressed proteins in the interaction of Paracoccidioides lutzii with human monocytes","authors":"Flamélia Carla Silva Oliveira , Wallace Felipe Blohem Pessoa , Joise Hander Mares , Herbert Pina Silva Freire , Ednara Almeida de Souza , Carlos Priminho Pirovani , Carla Cristina Romano","doi":"10.1016/j.riam.2020.09.006","DOIUrl":"10.1016/j.riam.2020.09.006","url":null,"abstract":"<div><h3>Background</h3><p>Fungi of the genus <span><em>Paracoccidioides</em></span> are the etiological agents of paracoccidioidomycosis, a highly prevalent mycosis in Latin America. Infection in humans occurs by the inhalation of conidia, which later revert to the form of yeast. In this context, macrophages are positioned as an important line of defense, assisting in the recognition and presentation of antigens, as well as producing reactive oxygen species that inhibit fungal spreading.</p></div><div><h3>Aims</h3><p>The objective of this study was to identify differentially expressed proteins during the interaction between <em>Paracoccidioides lutzii</em> Pb01 strain and human U937 monocytes.</p></div><div><h3>Methods</h3><p>Two-dimensional electrophoresis, combined with mass spectrometry, was used to evaluate the differential proteomic profiles of the fungus <em>P. lutzii</em> (Pb01) interacting with U937 monocytes.</p></div><div><h3>Results</h3><p>It was possible to identify 25 proteins differentially expressed by Pb01 alone and after interacting with U937 monocytes. Most of these proteins are directly associated with fungal metabolism for energy generation, such as glyceraldehyde-3-phosphate dehydrogenase, and intracellular adaptation to monocytes. Antioxidant proteins involved in the response to oxidative stress, such as peroxiredoxin, cytochrome, and peroxidase, were expressed in greater quantity in the interaction with monocytes, suggesting their association with survival mechanisms inside phagocytic cells. We also identified 12 proteins differentially expressed in monocytes before and after the interaction with the fungus; proteins involved in the reorganization of the cytoskeleton, such as vimentin, and proteins involved in the response to oxidative stress, such as glioxalase 1, were identified.</p></div><div><h3>Conclusions</h3><p>The results of this proteomic study of a <em>P. lutzii</em> isolate are novel, mimicking in vitro what occurs in human infections. In addition, the proteins identified may aid to understand fungal–monocyte interactions and the pathogenesis of paracoccidioidomycosis.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 159-167"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39642633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anidulafungin versus fluconazole in the treatment of Candida albicans chorioretinitis 阿尼杜冯宁与氟康唑治疗白色念珠菌绒毛膜视网膜炎的比较

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-10-01 DOI: 10.1016/j.riam.2021.05.002

Celia Ruiz-Arranz , Eugenio Pérez-Blázquez , Almudena De Pablo-Cabrera , Manuel Ferro-Osuna

Background

Candida albicans chorioretinitis is the most common cause of endogenous fungal endophthalmitis. Echinocandins are recommended as first-line therapy in the treatment of invasive candidiasis (IC), but in clinically stable patients with IC and endophthalmitis caused by Candida species susceptible to azole compounds these are the first-line treatment due to their better intraocular penetration.

Case report

A 42-year-old woman admitted to hospital for duodenal perforation after gastrointestinal surgery and treated with broad-spectrum antibiotics developed C. albicans candidemia. According to protocol, an antifungal treatment with anidulafungin was given. The patient presented no visual symptoms but on routinary ophthalmoscopic examination multiple bilateral chorioretinal lesions were observed. Systemic therapy was changed to fluconazole, with good systemic and ocular results.

Conclusions

Azole compounds are the first-line therapy for endophthalmitis associated with candidemia. However, clinical guidelines often propose echinocandins as the first option for IC. In some cases, C. albicans chorioretinitis will require a change in the systemic treatment to assure better intraocular penetration. According to the current evidence and our own experience, routine funduscopy is not necessary in all IC patients. However, we do recommend fundus examination in patients with visual symptoms or those unable to report them (paediatric patients and patients with an altered level of consciousness), and in those who are being treated with echinocandins in monotherapy.

背景:白色念珠菌绒毛膜视网膜炎是内源性真菌性眼内炎最常见的病因。棘白菌素被推荐作为治疗侵袭性念珠菌病(IC)的一线药物，但在临床稳定的IC患者和对唑类化合物敏感的念珠菌引起的眼内炎患者中，由于它们具有更好的眼内穿透性，因此它们是一线药物。病例报告:一名42岁女性在胃肠道手术后因十二指肠穿孔入院，并接受广谱抗生素治疗，并发白色念珠菌。根据治疗方案，给予阿尼杜冯宁抗真菌治疗。患者无视觉症状，但常规眼底检查发现双侧多处脉络膜视网膜病变。全身治疗改为氟康唑，全身和眼部效果良好。结论唑类药物是治疗念珠菌性眼内炎的一线药物。然而，临床指南经常建议棘白菌素作为IC的第一选择。在某些情况下，白色念珠菌性脉络膜视网膜炎需要改变全身治疗，以确保更好的眼内渗透。根据目前的证据和我们自己的经验，并不是所有的IC患者都需要常规的眼底检查。然而，我们确实建议对有视觉症状或无法报告这些症状的患者(儿科患者和意识水平改变的患者)以及正在单药治疗中使用棘白菌素的患者进行眼底检查。

{"title":"Anidulafungin versus fluconazole in the treatment of Candida albicans chorioretinitis","authors":"Celia Ruiz-Arranz , Eugenio Pérez-Blázquez , Almudena De Pablo-Cabrera , Manuel Ferro-Osuna","doi":"10.1016/j.riam.2021.05.002","DOIUrl":"10.1016/j.riam.2021.05.002","url":null,"abstract":"<div><h3>Background</h3><p><span><em>Candida albicans</em></span> chorioretinitis is the most common cause of endogenous fungal endophthalmitis. Echinocandins are recommended as first-line therapy in the treatment of invasive candidiasis (IC), but in clinically stable patients with IC and endophthalmitis caused by <em>Candida</em> species susceptible to azole compounds these are the first-line treatment due to their better intraocular penetration.</p></div><div><h3>Case <em>report</em></h3><p>A 42-year-old woman admitted to hospital for duodenal perforation after gastrointestinal surgery and treated with broad-spectrum antibiotics developed <em>C. albicans</em><span> candidemia<span>. According to protocol, an antifungal treatment with anidulafungin was given. The patient presented no visual symptoms but on routinary ophthalmoscopic examination multiple bilateral chorioretinal lesions were observed. Systemic therapy was changed to fluconazole, with good systemic and ocular results.</span></span></p></div><div><h3>Conclusions</h3><p>Azole compounds are the first-line therapy for endophthalmitis associated with candidemia. However, clinical guidelines often propose echinocandins as the first option for IC. In some cases, <em>C. albicans</em> chorioretinitis will require a change in the systemic treatment to assure better intraocular penetration. According to the current evidence and our own experience, routine funduscopy is not necessary in all IC patients. However, we do recommend fundus examination in patients with visual symptoms or those unable to report them (paediatric patients and patients with an altered level of consciousness), and in those who are being treated with echinocandins in monotherapy.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"38 4","pages":"Pages 188-191"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39718160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kerion Celsi caused by Trichophyton tonsurans with dermatophytid reaction 由癣毛癣菌引起的角膜炎伴皮癣反应

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-07-01 DOI: 10.1016/j.riam.2020.12.002

Elisabeth Gómez-Moyano , Ana María Fernández-Sánchez , Vicente Crespo-Erchiga , Leandro Martínez-Pilar

引用次数: 3

Nosemosis and the collapse of beehives 鼻塞病和蜂房坍塌

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2021-07-01 DOI: 10.1016/j.riam.2020.03.003

F. Javier Cabañes

引用次数: 0

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