Revista Iberoamericana De Micologia最新文献

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In vitro activity of juglone (5-hydroxy-1,4-naphthoquinone) against both fluconazole-resistant and susceptible Candida isolates 核桃酮(5-羟基-1,4-萘醌)对氟康唑耐药和敏感念珠菌的体外活性研究

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-04-01 DOI: 10.1016/j.riam.2022.01.004

Afsane Vaezi , Masoud Moghadaszadeh , Elahe Nasri , Shima Gharibi , Kambiz Diba , Adam Matkowski , Hamed Fakhim

Background

The rise in antifungal resistance and drug class limitations are causing higher morbidity and mortality rates all over the world. This issue highlights the urgent need for new and improved antifungal drugs with a novel target.

Aims

In order to evaluate whether juglone can be served as an alternative antifungal to cure drug-resistant Candida infections, we studied the in vitro susceptibility of juglone against fluconazole-susceptible and -resistance Candida isolates, alone and in combination.

Methods

Antifungal susceptibility testing was performed according to the CLSI (Clinical and Laboratory Standards Institute) guidelines.

Results

Juglone exhibited the highest minimal inhibitory concentration (MIC) values, followed by fluconazole and nystatin. Voriconazole showed significantly better antifungal activity than juglone, fluconazole, and nystatin, with MIC₅₀ and MIC₉₀ of 0.031 and 0.5 μg/mL. There were significant differences in MICs of fluconazole (p < 0.001) and juglone (p < 0.0003) between Candida albicans and the rest of the species. Combination of juglone with fluconazole revealed insignificant effects against fluconazole-susceptible and -resistant Candida isolates. Juglone increased the antifungal activity of fluconazole; however, no synergism effects were observed for any combination, and only an insignificant effect was found against all tested Candida species.

Conclusions

Although obtaining new antifungal drugs is a critical point, a completely novel approach should be implemented.

随着抗真菌药物耐药性的增加和药物种类的限制，全世界的发病率和死亡率都在上升。这一问题突出了迫切需要新的和改进的抗真菌药物与一个新的目标。目的为了评价核桃胶酮是否可以作为耐药念珠菌感染的替代抗真菌药物，我们研究了核桃胶酮对氟康唑敏感和耐药念珠菌的体外敏感性。方法按照临床与实验室标准协会(CLSI)指南进行真菌药敏试验。结果核桃酮最低抑菌浓度最高，氟康唑次之，制霉菌素次之。伏立康唑的抗真菌活性显著优于核桃酮、氟康唑和制霉菌素，MIC50和MIC90分别为0.031和0.5 μg/mL。氟康唑的mic差异有统计学意义(p <0.001)和朱格隆(p <0.0003)白色念珠菌与其他种类之间的差异。核桃酮与氟康唑联用对氟康唑敏感和耐药念珠菌的影响不显著。核桃酮能增强氟康唑的抗真菌活性;然而，任何组合均未观察到增效作用，对所有被试念珠菌只有不显著的作用。结论获得新的抗真菌药物是关键，但应采取全新的途径。

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引用次数: 1

Evaluation of the diagnostic accuracy of galactomannan from the bronchoalveolar lavage fluid of patients with suspected invasive pulmonary aspergillosis 支气管肺泡灌洗液半乳甘露聚糖诊断疑似侵袭性肺曲霉病的准确性评价

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-04-01 DOI: 10.1016/j.riam.2021.07.002

Rafaela Manzoni Bernardi , Sizuane Rieger Holler , Ellis Gabriela Correa de Almeida , Camila Anton , Felipe Dominguez Machado , Denise Rossato Silva

Background

Several studies to evaluate the accuracy of galactomannan (GM) in bronchoalveolar lavage fluid (BALF) as a diagnostic tool have been carried out; however, there are still controversies about the optimal cut-off point of BALF GM.

Aims

The objective of this study was to determine the diagnostic accuracy and the optimal cut-off point on BALF GM from patients with suspected invasive pulmonary aspergillosis (IPA) in a tertiary care hospital.

Methods

A cross-sectional study with 188 patients (≥18 years) that had undergone a bronchoscopy with BAL due to suspected IPA was carried out. IPA was diagnosed according to the EORTC/MSG guidelines.

Results

The optimal optical density cut-off point for BALF GM was 0.67, with sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 70%, 32.3%, and 100%, respectively.

Conclusions

BALF GM detection proved to be a useful supplementary technique in the early diagnosis of IPA in both neutropenic and non-neutropenic patients.

背景:一些研究评估了半乳甘露聚糖(GM)在支气管肺泡灌洗液(BALF)中作为诊断工具的准确性;然而，BALF GM的最佳分界点仍存在争议。目的本研究的目的是确定三级医院疑似侵袭性肺曲霉病(IPA)患者BALF GM的诊断准确性和最佳分界点。方法对188例因怀疑IPA而行BAL支气管镜检查的患者(≥18岁)进行横断面研究。IPA是根据EORTC/MSG指南诊断的。结果BALF GM的最佳光密度截止点为0.67，敏感性为100%，特异性为70%，阳性预测值为32.3%，阴性预测值为100%。结论balf GM检测在中性粒细胞减少和非中性粒细胞减少患者IPA的早期诊断中是一种有用的辅助技术。

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引用次数: 0

Possible Trichosporon asahii urinary tract infection in a critically ill COVID-19 patient COVID-19危重症患者可能的朝日毛孢丝状体尿路感染

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-04-01 DOI: 10.1016/j.riam.2022.05.001

Marco Vianello , Daniel F.F. de Jesus , Jorge M. Sampaio , Guilherme M. de Oliveira , Nilton Lincopan , Kelly Ishida

Background

Trichosporon asahii, an emerging fungal pathogen, has been frequently associated with invasive infections in critically ill patients.

Case report

A 74-year-old male patient diagnosed with COVID-19 was admitted to an Intensive Care Unit (ICU). During hospitalization, the patient displayed episodes of bacteremia by Staphylococcus haemolyticus and a possible urinary tract infection by T. asahii. While the bacterial infection was successfully treated using broad-spectrum antibiotics, the fungal infection in the urinary tract was unsuccessfully treated with anidulafungin and persisted until the patient died.

Conclusions

With the evolving COVID-19 pandemic, invasive fungal infections have been increasingly reported, mainly after taking immunosuppressant drugs associated with long-term broad-spectrum antibiotic therapy. Although Candida and Aspergillus are still the most prevalent invasive fungi, T. asahii and other agents have emerged in critically ill patients. Therefore, a proper surveillance and diagnosing any fungal infection are paramount, particularly in COVID-19 immunocompromised populations.

背景:朝日毛磷霉是一种新兴的真菌病原体，常与危重患者的侵袭性感染有关。病例报告一名74岁的男性患者被诊断为COVID-19，并被送入重症监护病房(ICU)。住院期间，患者出现溶血葡萄球菌引起的菌血症发作，并可能出现asahi t尿路感染。虽然使用广谱抗生素成功治疗了细菌感染，但尿路真菌感染无法用阿尼杜冯宁治疗，并持续到患者死亡。结论随着COVID-19大流行的发展，侵袭性真菌感染的报道越来越多，主要是在长期服用免疫抑制药物和广谱抗生素治疗后。虽然念珠菌和曲霉菌仍然是最普遍的侵袭性真菌，但朝日弧菌和其他病原体已经出现在危重患者中。因此，适当监测和诊断任何真菌感染至关重要，特别是在COVID-19免疫功能低下人群中。

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引用次数: 0

Epizootic lymphangitis: A neglected disease of working equids 兽疫性淋巴管炎:一种被劳动马匹忽视的疾病

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.11.001

F. Javier Cabañes

引用次数: 0

Lodderomyces elongisporus: ¿agente causal de vaginitis? 长孢Lodderomyces elongisporus:阴道炎的病原体?

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.11.002

Laura Sante , Patricia Capón , Amparo Coira Nieto , Pilar Alonso-García

引用次数: 1

Ángela Restrepo Moreno: The wise woman par excellence! 莫雷诺:The wiseÁngela雷斯特雷波出类拔萃的女人!

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2022.03.001

引用次数: 0

Estado actual de la histoplasmosis diseminada progresiva en pacientes con infección por el VIH en un hospital de tercer nivel en Perú 秘鲁一家三级医院艾滋病毒感染患者进行性传播组织胞浆菌病的现状

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.10.001

Omayra Chincha , Beatriz Bustamante

Background

Progressive disseminated histoplasmosis (PDH) is an endemic disease in most of Latin America, especially among patients with HIV. There are few reports about this disease in Peru.

Aims

To describe the clinical, epidemiological and mycological features of patients with PDH and HIV evaluated in a tertiary hospital.

Methods

A retrospective study to find out the data of patients diagnosed with PDH and HIV in the period 2000–2019 was carried out. For the statistical analysis of quantitative variables, measures of central tendency and dispersion were used; for the qualitative variables, absolute and relative frequencies were used.

Results

Forty-three male patients with PDH were diagnosed in the study period, with a median age of 33 years (IQR: 29–38 years) and a median CD₄ lymphocytes count of 39 cells/mm³ (IQR: 20–83 cells/mm³). Eighty six percent of the patients were born or had travelled to the jungle, 58.1% were alcohol users and 16.1% had a history of pulmonary tuberculosis. When compared to histopathology, the culture had a better sensitivity to achieve a diagnosis (p < 0.05).

Conclusions

Peruvian patients with PDH and HIV infection were mainly young male adults that were born or had travelled to the jungle, with a CD₄ count below 100 cells/mm³. In patients with the described characteristics it would be advisable to check for PDH. Implementing rapid diagnostic tests is also necessary.

进展性播散性组织胞浆菌病(PDH)是拉丁美洲大部分地区的一种地方病，特别是在艾滋病毒感染者中。秘鲁很少有关于这种疾病的报道。目的了解某三级医院PDH合并HIV患者的临床、流行病学和真菌学特征。方法对2000-2019年诊断为PDH和HIV的患者资料进行回顾性研究。定量变量的统计分析采用集中趋势和离散度度量;对于定性变量，使用绝对频率和相对频率。结果研究期间确诊的男性PDH患者43例，中位年龄33岁(IQR: 29-38岁)，中位CD4淋巴细胞计数39个细胞/mm3 (IQR: 20-83个细胞/mm3)。86%的患者出生或去过丛林，58.1%的患者酗酒，16.1%的患者有肺结核史。与组织病理学相比，培养具有更好的诊断敏感性(p <0.05)。结论秘鲁PDH合并HIV感染患者以出生或去过丛林的年轻成年男性为主，CD4细胞计数低于100细胞/mm3。对于具有上述特征的患者，建议检查PDH。实施快速诊断测试也是必要的。

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引用次数: 1

Unusual form of histoplasmosis in dogs and cats 狗和猫的组织胞浆菌病的不寻常形式

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.09.001

F. Javier Cabañes

引用次数: 0

Potencial de la levadura oleaginosa Clavispora lusitaniae Hi2 en la conversión de residuos agroindustriales a lípidos 油料酵母lusitaniae Hi2在农用工业废弃物转化为脂类方面的潜力

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2021.07.001

Aime Frida Moran-Mejía , Rosa Angélica Guillén-Garcés , Gabriela Eleonora Moeller-Chávez , Jesús Hernández-Romano , María Inés Chávez-Béjar , Clarita Olvera-Carranza , Luis Gerardo Treviño-Quintanilla

Background

Single-cell oils obtained from oleaginous microorganisms by using lignocellulosic waste hydrolysates are an alternative for producing biodiesel.

Aims

To isolate a yeast strain able to produce lipids from centrifuged nejayote (CN), hydrolyzed nejayote solids (HNS) and hydrolyzed sugarcane bagasse (HSB).

Methods

In order to identify the yeasts recovered, 26S ribosomal DNA was sequenced. The metabolic profile was assessed by using API20C AUX strips. The nutritional characterization of CN, HNS and HSB was performed by quantifying reducing sugars, total carbohydrates, starch, protein and total nitrogen. The biomass and lipid production ability were evaluated by performing growth kinetics of Clavispora lusitaniae Hi2 in combined culture media.

Results

Six oleaginous yeast strains were isolated and identified, selecting C. lusitaniae Hi2 to study its lipids production by using nejayote. The C. lusitaniae Hi2 strain can use glucose, xylose, arabinose, galactose and cellobiose as carbon sources. Cultures of C. lusitaniae Hi2 presented the best biomass (5.6±0.28 g/L) and lipid production (0.99±0.09 g/L) at 20 h of incubation with the CN:HNS media in the 25:75 and 50:50 ratios, respectively.

Conclusions

The use of CN, HNS and HSB for the growth of C. lusitaniae Hi2 is an option to take advantage of these agro-industrial residues and generate compounds of biotechnological interest.

利用木质纤维素废物水解物从产油微生物中获得的单细胞油是生产生物柴油的一种替代方法。目的分离一株能从离心内jayote (CN)、水解内jayote固体(HNS)和水解甘蔗渣(HSB)中产生脂质的酵母菌株。方法对回收的酵母菌进行26S核糖体DNA测序鉴定。采用API20C AUX试纸评估代谢谱。通过定量测定还原糖、总碳水化合物、淀粉、蛋白质和总氮，对CN、HNS和HSB进行营养鉴定。通过菌丝体Hi2在组合培养基上的生长动力学，评价菌丝体的生物量和产脂能力。结果分离鉴定了6株产油酵母菌株，选取C. lusitaniae Hi2对其利用nejayote产脂进行了研究。C. lusitaniae Hi2菌株可以利用葡萄糖、木糖、阿拉伯糖、半乳糖和纤维素糖作为碳源。在CN:HNS培养基中以25:75和50:50的比例培养20 h时，lusitaniae Hi2的生物量(5.6±0.28 g/L)和脂质产量(0.99±0.09 g/L)最高。结论利用CN、HNS和HSB培养卢西塔尼亚C. Hi2是一种利用这些农工残留物产生具有生物技术价值的化合物的选择。

{"title":"Potencial de la levadura oleaginosa Clavispora lusitaniae Hi2 en la conversión de residuos agroindustriales a lípidos","authors":"Aime Frida Moran-Mejía , Rosa Angélica Guillén-Garcés , Gabriela Eleonora Moeller-Chávez , Jesús Hernández-Romano , María Inés Chávez-Béjar , Clarita Olvera-Carranza , Luis Gerardo Treviño-Quintanilla","doi":"10.1016/j.riam.2021.07.001","DOIUrl":"https://doi.org/10.1016/j.riam.2021.07.001","url":null,"abstract":"<div><h3>Background</h3><p>Single-cell oils obtained from oleaginous microorganisms by using lignocellulosic waste hydrolysates are an alternative for producing biodiesel.</p></div><div><h3>Aims</h3><p>To isolate a yeast strain able to produce lipids from centrifuged nejayote (CN), hydrolyzed nejayote solids (HNS) and hydrolyzed sugarcane bagasse (HSB).</p></div><div><h3>Methods</h3><p>In order to identify the yeasts recovered, 26S ribosomal DNA was sequenced. The metabolic profile was assessed by using API20C AUX strips. The nutritional characterization of CN, HNS and HSB was performed by quantifying reducing sugars, total carbohydrates, starch, protein and total nitrogen. The biomass and lipid production ability were evaluated by performing growth kinetics of <em>Clavispora lusitaniae</em> Hi2 in combined culture media.</p></div><div><h3>Results</h3><p>Six oleaginous yeast strains were isolated and identified, selecting <em>C. lusitaniae</em> Hi2 to study its lipids production by using nejayote. The <em>C. lusitaniae</em> Hi2 strain can use glucose, xylose, arabinose, galactose and cellobiose as carbon sources. Cultures of <em>C. lusitaniae</em> Hi2 presented the best biomass (5.6<span>±</span>0.28 g/L) and lipid production (0.99<span>±</span>0.09 g/L) at 20 h of incubation with the CN:HNS media in the 25:75 and 50:50 ratios, respectively.</p></div><div><h3>Conclusions</h3><p>The use of CN, HNS and HSB for the growth of <em>C. lusitaniae</em> Hi2 is an option to take advantage of these agro-industrial residues and generate compounds of biotechnological interest.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 1","pages":"Pages 6-15"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92266552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Lethal destructive sinusopathy due to amphotericin B-resistant Aspergillus flavus: A case report 两性霉素b耐药黄曲霉致致致命性破坏性鼻窦病1例。

IF 1.9 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia

Pub Date : 2022-01-01 DOI: 10.1016/j.riam.2022.01.001

Tiago Luiz Lagedo Ferraz , Eduardo Marques Araújo , Rodolfo Froes Calixto , Monique Lima Martins Sampaio , Luciana Rezende Bandeira de Mello , Kaliny Benicio Torres , Rossana de Aguiar Cordeiro , Patrice Le Pape , Reginaldo Gonçalves de Lima-Neto

Background

The prevalence of pulmonary aspergillosis and the importance of its early diagnosis are recognized. However, non-pulmonary involvement, including the sinuses region, is not frequently reported, and an infection in this area can affect all paranasal sinuses (pansinusopathy), being a rare pathology that affects immunocompromised hosts. Recent studies have highlighted the occurrence of Aspergillus flavus resistant to antifungal therapy. Therefore, a nasal sinus infection by resistant Aspergillus strains in immunocompromised patients may be linked to a high risk of lethality.

Case report

We are reporting a resistant A. flavus infection in an allogeneic hematopoietic stem cell transplant recipient with episodes of febrile neutropenia, and prolonged use of various antibacterial drugs and antifungal prophylaxis. The patient underwent brain magnetic resonance, which showed the presence of pansinusopathy, and presented necrosis in the left nasal region. Direct microscopic examination of a sample taken from the nasal mucosa revealed the presence of septate hyphae and conidiophores resembling those of A. flavus, that species being the identification achieved with MALDI-TOF MS. Antifungigram was performed by microdilution in broth (EUCAST-E.DEF. 9.3.2) and E-test, and resistance to amphotericin B was shown in both tests. The patient died after septic shock and hemorrhage.

Conclusions

Invasive fungal infections due to amphotericin-B resistant A. flavus may lead to the death of the patient due to an ineffective therapeutic management. Therefore, antifungal susceptibility testing are of utmost importance for administering the proper treatment.

背景肺曲霉病的流行及其早期诊断的重要性已得到认识。然而，包括鼻窦区域在内的非肺部受累并不常见，该区域的感染可影响所有鼻窦(泛鼻窦病)，这是一种罕见的影响免疫功能低下宿主的病理。最近的研究强调了黄曲霉抗真菌治疗的发生。因此，免疫功能低下患者的耐药曲霉菌株引起的鼻窦感染可能与高致死率有关。病例报告:我们报告一例异体造血干细胞移植受者发生耐药性黄芽孢杆菌感染，伴发热性中性粒细胞减少发作，长期使用各种抗菌药物和抗真菌预防。患者行脑磁共振检查，显示有全窦病变，左鼻区出现坏死。对鼻黏膜标本进行直接显微镜检查，发现存在类似黄芽孢杆菌的分离菌丝和分生孢子，该菌种是用MALDI-TOF ms鉴定的，用EUCAST-E.DEF在肉汤中微量稀释进行抗真菌图测定。9.3.2)和e检验均显示对两性霉素B耐药。病人死于感染性休克和出血。结论两性霉素- b耐药黄曲霉引起的侵袭性真菌感染，如果治疗不善，可能导致患者死亡。因此，抗真菌药敏试验是最重要的给予适当的治疗。

{"title":"Lethal destructive sinusopathy due to amphotericin B-resistant Aspergillus flavus: A case report","authors":"Tiago Luiz Lagedo Ferraz , Eduardo Marques Araújo , Rodolfo Froes Calixto , Monique Lima Martins Sampaio , Luciana Rezende Bandeira de Mello , Kaliny Benicio Torres , Rossana de Aguiar Cordeiro , Patrice Le Pape , Reginaldo Gonçalves de Lima-Neto","doi":"10.1016/j.riam.2022.01.001","DOIUrl":"10.1016/j.riam.2022.01.001","url":null,"abstract":"<div><h3>Background</h3><p>The prevalence of pulmonary aspergillosis and the importance of its early diagnosis are recognized. However, non-pulmonary involvement, including the sinuses region, is not frequently reported, and an infection in this area can affect all paranasal sinuses (pansinusopathy), being a rare pathology that affects immunocompromised hosts. Recent studies have highlighted the occurrence of <span><em>Aspergillus</em><em> flavus</em></span> resistant to antifungal therapy. Therefore, a nasal sinus infection by resistant <em>Aspergillus</em> strains in immunocompromised patients may be linked to a high risk of lethality.</p></div><div><h3>Case report</h3><p>We are reporting a resistant <em>A. flavus</em> infection in an allogeneic hematopoietic stem cell transplant recipient with episodes of febrile neutropenia, and prolonged use of various antibacterial drugs and antifungal prophylaxis. The patient underwent brain magnetic resonance, which showed the presence of pansinusopathy, and presented necrosis in the left nasal region. Direct microscopic examination of a sample taken from the nasal mucosa revealed the presence of septate hyphae and conidiophores resembling those of <em>A. flavus</em><span>, that species being the identification achieved with MALDI-TOF MS. Antifungigram was performed by microdilution in broth (EUCAST-E.DEF. 9.3.2) and E-test, and resistance to amphotericin B was shown in both tests. The patient died after septic shock and hemorrhage.</span></p></div><div><h3>Conclusions</h3><p>Invasive fungal infections due to amphotericin-B resistant <em>A. flavus</em> may lead to the death of the patient due to an ineffective therapeutic management. Therefore, antifungal susceptibility testing are of utmost importance for administering the proper treatment.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"39 1","pages":"Pages 21-24"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42474513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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