This research reported the Purifi cation and Characterization of Thermostable Amylase from a strain of T. Thalpophilus KSV 17. The result showed that the purifi ed enzyme specifi c activity of 145.80 U mg –1 , this was an increase of 21 fold than the crude enzyme extract. The analysis of SDS- polyacrylamide gel electrophoresis showed that the molecular weight of the enzyme was 52 kDa. The Optimum pH of the purifi ed enzyme showed maximum activity at pH 7.0, but the enzyme was stable in the pH range of 5.5–7.0. The optimum temperature of the purifi ed enzyme was 85°C in absence of 10 mM CaCl 2 while 90°C in presence of 10 mM CaCl 2., K m and V max values for the purifi ed enzyme were calculated as 5.2 mg ml –1 , 0.45 mg ml –1 /minute respectively. The thermal stability of the purifi ed enzyme at 80°C in absence of CaCl 2. and 85°C in presence of CaCl 2 . The purifi ed enzyme mostly inhibited by diethyl pyrocarbonate and N-bromosuccinimide and at 5 mM conc. Ca 2+ , Na + and Mg 2+
本研究报道了一株thalpopophilus KSV 17耐热淀粉酶的纯化及特性。结果表明，纯化后的酶比活性为145.80 U mg -1，比粗酶提取物提高了21倍。SDS-聚丙烯酰胺凝胶电泳分析表明，该酶分子量为52 kDa。纯化酶的最适pH值为7.0，酶活性在5.5 ~ 7.0范围内稳定。纯化后的酶在不含10 mM氯化钙时的最适温度为85℃，存在10 mM氯化钙时的最适温度为90℃。计算纯化酶的K、m、V最大值分别为5.2 mg ml -1、0.45 mg ml -1 /min。在没有氯化钙的情况下，纯化酶在80℃时的热稳定性。在cacl2存在下达到85℃。纯化后的酶主要受焦碳酸二乙酯和n -溴琥珀酰亚胺的抑制，浓度为5 mM。ca2 +， Na +和mg2 +
{"title":"Purification and characterization of thermostable amylase from a strain of thermoactinomyces thalpophilus KSV 17","authors":"K. S. Rao, P. Ellaiah, K. V. Biradar","doi":"10.5530/RJPS.2012.1.12","DOIUrl":"https://doi.org/10.5530/RJPS.2012.1.12","url":null,"abstract":"This research reported the Purifi cation and Characterization of Thermostable Amylase from a strain of T. Thalpophilus KSV 17. The result showed that the purifi ed enzyme specifi c activity of 145.80 U mg –1 , this was an increase of 21 fold than the crude enzyme extract. The analysis of SDS- polyacrylamide gel electrophoresis showed that the molecular weight of the enzyme was 52 kDa. The Optimum pH of the purifi ed enzyme showed maximum activity at pH 7.0, but the enzyme was stable in the pH range of 5.5–7.0. The optimum temperature of the purifi ed enzyme was 85°C in absence of 10 mM CaCl 2 while 90°C in presence of 10 mM CaCl 2., K m and V max values for the purifi ed enzyme were calculated as 5.2 mg ml –1 , 0.45 mg ml –1 /minute respectively. The thermal stability of the purifi ed enzyme at 80°C in absence of CaCl 2. and 85°C in presence of CaCl 2 . The purifi ed enzyme mostly inhibited by diethyl pyrocarbonate and N-bromosuccinimide and at 5 mM conc. Ca 2+ , Na + and Mg 2+","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"9 3","pages":"83-89"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91486913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper discusses the theory of mucoadhesion along with various approaches to improve nasal absorption by the use of mucoadhesive polymers and absorption enhancers. An account of various mucoadhesive polymers is also given. A note on absorption enhancers has been included. Finally the growing market for nasal drug delivery is discussed.
{"title":"Nasal drug delivery–a review","authors":"T. Deshpande, R. Masareddy, A. Patil","doi":"10.5530/RJPS.2012.1.4","DOIUrl":"https://doi.org/10.5530/RJPS.2012.1.4","url":null,"abstract":"This paper discusses the theory of mucoadhesion along with various approaches to improve nasal absorption by the use of mucoadhesive polymers and absorption enhancers. An account of various mucoadhesive polymers is also given. A note on absorption enhancers has been included. Finally the growing market for nasal drug delivery is discussed.","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"18 1","pages":"24-37"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81726663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
From the petroleum ether extract of the roots of Carissa spinarum Linn. (Apocynaceae), six compounds namely stigmasterol, ursolic acid, lupeol, campesterol, 17-hydroxy-11-oxo-nor-β-amyrone and urs-12-ene-3β, 22β-diol17-carboxylic acid have been isolated by column chromatography. Their structures were characterized by m.p., IR, 1 HNMR, 13 CNMR and mass spectral data. However, the compounds stigmasterol, campesterol, 17-hydroxy11-oxo-nor-β-amyrone and urs-12-ene-3β, 22β-diol-17-carboxylic acid were reported for the fi rst time from the root of this plant.
{"title":"Phytochemical investigation of root extract of the plant Carissa spinarum","authors":"K. Hegde, Satyanarayana D, Arun B. Joshi","doi":"10.5530/RJPS.2012.1.7","DOIUrl":"https://doi.org/10.5530/RJPS.2012.1.7","url":null,"abstract":"From the petroleum ether extract of the roots of Carissa spinarum Linn. (Apocynaceae), six compounds namely stigmasterol, ursolic acid, lupeol, campesterol, 17-hydroxy-11-oxo-nor-β-amyrone and urs-12-ene-3β, 22β-diol17-carboxylic acid have been isolated by column chromatography. Their structures were characterized by m.p., IR, 1 HNMR, 13 CNMR and mass spectral data. However, the compounds stigmasterol, campesterol, 17-hydroxy11-oxo-nor-β-amyrone and urs-12-ene-3β, 22β-diol-17-carboxylic acid were reported for the fi rst time from the root of this plant.","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"130 1","pages":"55-59"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77081700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nanobiotechnology is a new technology concerned specifi cally with the functionalism and modifi cation of chemical-physical structures on a biomolecular scale, and also is the application of nanotechnology to the life sciences. Nanotechnology for biotechnology and pharmaceutical applications has progressed from the concept stage to commercialization. Nanobiotechnology represents the future of medicine and healthcare. Various physical, chemical, electrical tools and methods used to investigate biological nanoobjects include optical tools, nanoforce and imaging, surface methods, mass spectrometry and microfl udics. Its application has an impact on diagnostics, drug delivery as well as drug discovery. Nanobiotechnology focuses on various areas such as nanobiotechnology and cancer, drug discovery and tools, and nanobiotechnology and medicine. Applications are emerging from all branches of nanobiotechnology in medicine and pharmacy. Several technologies including nanoparticles and nanodevices such as nanobiosensors and nanobiochips have been used to improve drug discovery and development. Some nanosubstances such as fullerenes and dendrimers/biodendrimers could be potential drugs for the future. Moreover, nanobiotechnology has the potential for combining drug design and drug delivery. However, limitations of the available nanoparticles still to be resolved for their application in the drug-discovery studies exist. The benefi ts of nanotechnology are enormous and so these benefi ts should be maximized while efforts are made to reduce the risks.
{"title":"Nanobiotechnology: An overview of drug discovery, delivery and development","authors":"B. S. Sekhon","doi":"10.5530/RJPS.2012.1.3","DOIUrl":"https://doi.org/10.5530/RJPS.2012.1.3","url":null,"abstract":"Nanobiotechnology is a new technology concerned specifi cally with the functionalism and modifi cation of chemical-physical structures on a biomolecular scale, and also is the application of nanotechnology to the life sciences. Nanotechnology for biotechnology and pharmaceutical applications has progressed from the concept stage to commercialization. Nanobiotechnology represents the future of medicine and healthcare. Various physical, chemical, electrical tools and methods used to investigate biological nanoobjects include optical tools, nanoforce and imaging, surface methods, mass spectrometry and microfl udics. Its application has an impact on diagnostics, drug delivery as well as drug discovery. Nanobiotechnology focuses on various areas such as nanobiotechnology and cancer, drug discovery and tools, and nanobiotechnology and medicine. Applications are emerging from all branches of nanobiotechnology in medicine and pharmacy. Several technologies including nanoparticles and nanodevices such as nanobiosensors and nanobiochips have been used to improve drug discovery and development. Some nanosubstances such as fullerenes and dendrimers/biodendrimers could be potential drugs for the future. Moreover, nanobiotechnology has the potential for combining drug design and drug delivery. However, limitations of the available nanoparticles still to be resolved for their application in the drug-discovery studies exist. The benefi ts of nanotechnology are enormous and so these benefi ts should be maximized while efforts are made to reduce the risks.","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"11 1","pages":"14-24"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87124406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Every work must have a starting point, and scientifi c research is no exception. Reviewing literature is an initial step in undertaking scientifi c research. Reviewing literature, as an ongoing process, provides a critical overview on the topic of interest and keeps the researcher up-to-date to pursue novel research plans. While reviewing literature helps early career investigators identify their research niche, established researchers review the literature to propagate the state of the art progress/opinions in their fi eld of expertise. Most of these reviews often guide the general research community. Various formats are often adopted in writing a review depending on the context, audience, depth/complexity of subject and extent of commercial value. The primary goal is to provide a critical and simplifi ed analysis of the facts. Essentially, every researcher should adapt to these formats in a unique way and should try to develop their own style of reviewing the literature. In this review, we will describe some of the essential steps to approaching various formats of reviewing the literature.
{"title":"Knowing the known to understand the unknown- A systematic approach to reviewing the scientific literature","authors":"Y. Kuberappa, Arun H. S. Kumar","doi":"10.5530/RJPS.2012.1.1","DOIUrl":"https://doi.org/10.5530/RJPS.2012.1.1","url":null,"abstract":"Every work must have a starting point, and scientifi c research is no exception. Reviewing literature is an initial step in undertaking scientifi c research. Reviewing literature, as an ongoing process, provides a critical overview on the topic of interest and keeps the researcher up-to-date to pursue novel research plans. While reviewing literature helps early career investigators identify their research niche, established researchers review the literature to propagate the state of the art progress/opinions in their fi eld of expertise. Most of these reviews often guide the general research community. Various formats are often adopted in writing a review depending on the context, audience, depth/complexity of subject and extent of commercial value. The primary goal is to provide a critical and simplifi ed analysis of the facts. Essentially, every researcher should adapt to these formats in a unique way and should try to develop their own style of reviewing the literature. In this review, we will describe some of the essential steps to approaching various formats of reviewing the literature.","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"16 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83450915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Girish Bolakatti, Manjunatha S. Katagi, Mamledesai Sn, Sujatha Ml, P. Dabadi, N. Miskin
A series of new 2-quinolone derivatives were synthesized, purifi ed and characterized on the basis of IR, 1 H NMR, 13
合成了一系列新的2-喹诺酮类衍生物，对其进行了纯化，并通过IR、1h NMR、13进行了表征
{"title":"Synthesis and antimicrobial activity of 4-hydroxy-1-methyl/phenyl-3- (substituted anilinoacetyl) quinolin-2(1H)-one","authors":"Girish Bolakatti, Manjunatha S. Katagi, Mamledesai Sn, Sujatha Ml, P. Dabadi, N. Miskin","doi":"10.5530/RJPS.2012.1.8","DOIUrl":"https://doi.org/10.5530/RJPS.2012.1.8","url":null,"abstract":"A series of new 2-quinolone derivatives were synthesized, purifi ed and characterized on the basis of IR, 1 H NMR, 13","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"72 1","pages":"60-66"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83908996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Immune activation is an effective as well as a protective and novel approach against emerging infectious diseases. Traditionally Pongamia glabra Vent claimed to cure infectious diseases needs scientifi c validation as immunomodulatory agent. Methanolic extracts of seeds and barks at the doses of 250 mg/kg and 500 mg/kg (per oral) of Pongamia glabra Vent. were studied for the assessment of immunomodulatory activity on cyclophosphamide induced immunosuppression in mice. The activity was assessed by determining the RBC, Hb%, platelet, total WBC and differential counts. Methanolic extracts of seeds and barks of Pongamia glabra Vent. showed dose dependent highly signifi cant counteracting effect (p<0.001) to cyclophosphamide induced reduction in total WBC and platelet counts and signifi cant (p<0.01) effect to that of reduction in RBC counts, Hb% and DLC. The signifi cant inmmunostimulant effect of the methanolic extracts of Pongamia glabra Vent. seeds and bark on cyclophosphamide induced myelosuppression may be attributed towards the collective presence of saponins, sterols, tannins and fl avonoids in the extracts.
{"title":"Immunomodulatory activity of methanolic extracts of Pongamia glabra Vent. seeds and bark in cyclophosphamide induced mice","authors":"S. Heroor, Arunkumar Beknal, N. Mahurkar","doi":"10.5530/RJPS.2012.1.10","DOIUrl":"https://doi.org/10.5530/RJPS.2012.1.10","url":null,"abstract":"Immune activation is an effective as well as a protective and novel approach against emerging infectious diseases. Traditionally Pongamia glabra Vent claimed to cure infectious diseases needs scientifi c validation as immunomodulatory agent. Methanolic extracts of seeds and barks at the doses of 250 mg/kg and 500 mg/kg (per oral) of Pongamia glabra Vent. were studied for the assessment of immunomodulatory activity on cyclophosphamide induced immunosuppression in mice. The activity was assessed by determining the RBC, Hb%, platelet, total WBC and differential counts. Methanolic extracts of seeds and barks of Pongamia glabra Vent. showed dose dependent highly signifi cant counteracting effect (p<0.001) to cyclophosphamide induced reduction in total WBC and platelet counts and signifi cant (p<0.01) effect to that of reduction in RBC counts, Hb% and DLC. The signifi cant inmmunostimulant effect of the methanolic extracts of Pongamia glabra Vent. seeds and bark on cyclophosphamide induced myelosuppression may be attributed towards the collective presence of saponins, sterols, tannins and fl avonoids in the extracts.","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"53 1","pages":"74-77"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81808756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peptic ulcer is regarded as a multifactorial gastrointestinal disorder in its pathophysiology including free radical generations. Thereby free radical scavengers can play an important role in such diseases. The methanolic extract of Corms of Amorphophallus paeoniifolius exhibited remarkable anti-oxidant activity in various In-vitro models. Further, preliminary phytochemical screening revealed the presence of polyphenolic compounds; fl avonoids and tannins which are known to possess anti-ulcer activity. In light of these fi nding, it was under taken to investigate the gastroprotective activity of methanolic extract gainst NSAID–Indomethacin (30 mg/kg p.o.) induced gastotoxicity in Wistar albino rats wherein the animals were orally administered with two different doses of test extract (250 and 500 mg/kg b.w.) or with reference drug Lansoprozole (8 mg/kg p.o.). Animals were analyzed for Ulcer score, and in vitro estimation of GSH and LPO. Extract showed signifi cant (p<0.001) reduction in ulcer index in dose dependent manner along with signifi cant restoration of protective-GSH levels and suppression of LPO levels in tissues, in comparison with Standard. It was concluded that gastroprotective effect possessed by test extract may be offered by its bioactive phytoantioxidant constituents mainly the nine polyphenolics including Quercetin and Gallic acid revealed in HPTLC analysis.
{"title":"Evaluation of gastroprotective ability of Amorphophallus paeoniifolius corms against indomethacin induced gastric ulcers","authors":"Nataraj Hn, R. Murthy, R. Setty","doi":"10.5530/RJPS.2012.1.9","DOIUrl":"https://doi.org/10.5530/RJPS.2012.1.9","url":null,"abstract":"Peptic ulcer is regarded as a multifactorial gastrointestinal disorder in its pathophysiology including free radical generations. Thereby free radical scavengers can play an important role in such diseases. The methanolic extract of Corms of Amorphophallus paeoniifolius exhibited remarkable anti-oxidant activity in various In-vitro models. Further, preliminary phytochemical screening revealed the presence of polyphenolic compounds; fl avonoids and tannins which are known to possess anti-ulcer activity. In light of these fi nding, it was under taken to investigate the gastroprotective activity of methanolic extract gainst NSAID–Indomethacin (30 mg/kg p.o.) induced gastotoxicity in Wistar albino rats wherein the animals were orally administered with two different doses of test extract (250 and 500 mg/kg b.w.) or with reference drug Lansoprozole (8 mg/kg p.o.). Animals were analyzed for Ulcer score, and in vitro estimation of GSH and LPO. Extract showed signifi cant (p<0.001) reduction in ulcer index in dose dependent manner along with signifi cant restoration of protective-GSH levels and suppression of LPO levels in tissues, in comparison with Standard. It was concluded that gastroprotective effect possessed by test extract may be offered by its bioactive phytoantioxidant constituents mainly the nine polyphenolics including Quercetin and Gallic acid revealed in HPTLC analysis.","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"85 1","pages":"67-73"},"PeriodicalIF":0.0,"publicationDate":"2012-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83901382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Nayak, V. N. Swamy, A. Senthil, Jismon Jose, M. Mahin, R. Mahalaxmi
A B S T R A C T Over the past three decades, orodispersible tablets have gained considerable attention as a preferred alternative to conventional tablets and capsules due to better patient compliance. Rizatriptan Benzoate is potent anti migraine drug having agonist activity at the 5hydroxytryptamine (5-HT) 1B and (5-HT)1D receptor. It commonly used for relief of headaches in treatment of migraine. Conventional tablets of Rizatriptan Benzoate are not capable of rapid action, which is required for immediate relief from migraine pain. Marketed freeze dried tablet of Rizatriptan Benzoate is available. Freeze drying is cumbersome and it yields a fragile and hygroscopic product. Thus, the present investigation deals with development of Orodispersible tablets of Rizatriptan Benzoate to produce the intended benefits. Orodispersible tablets of Rizatriptan Benzoate were prepared using superdisintegrants viz; crospovidone, croscarmellose sodium and sodium starch glycolate using the direct compression method. The tablets prepared were evaluated for thickness, uniformity of weight, hardness, friability, wetting time, in vitro disintegration time and in vitro dissolution time. The tablets disintegrated within 21 to 75 s. Almost 95% of drug was released from all formulations within 15 min. The formulation containing 6% of croscarmellose sodium (F6) was found to give the best results. Apart from fulfilling all official and other specifications, the tablets exhibited higher rate of release. The stability studies were performed as per ICH guidelines. The Optimized formulation (F6) showed no significant variations for the tablets parameters and it was stable for the specified time period. It was concluded the Orodispersible tablets for Rizatriptan Benzoate can be formulated for emergency treatment of migraine.
{"title":"Formulation and Evaluation of Melt-In-Mouth Tablets of Rizatriptan Benzoate","authors":"R. Nayak, V. N. Swamy, A. Senthil, Jismon Jose, M. Mahin, R. Mahalaxmi","doi":"10.5530/RJPS.2011.3.3","DOIUrl":"https://doi.org/10.5530/RJPS.2011.3.3","url":null,"abstract":"A B S T R A C T Over the past three decades, orodispersible tablets have gained considerable attention as a preferred alternative to conventional tablets and capsules due to better patient compliance. Rizatriptan Benzoate is potent anti migraine drug having agonist activity at the 5hydroxytryptamine (5-HT) 1B and (5-HT)1D receptor. It commonly used for relief of headaches in treatment of migraine. Conventional tablets of Rizatriptan Benzoate are not capable of rapid action, which is required for immediate relief from migraine pain. Marketed freeze dried tablet of Rizatriptan Benzoate is available. Freeze drying is cumbersome and it yields a fragile and hygroscopic product. Thus, the present investigation deals with development of Orodispersible tablets of Rizatriptan Benzoate to produce the intended benefits. Orodispersible tablets of Rizatriptan Benzoate were prepared using superdisintegrants viz; crospovidone, croscarmellose sodium and sodium starch glycolate using the direct compression method. The tablets prepared were evaluated for thickness, uniformity of weight, hardness, friability, wetting time, in vitro disintegration time and in vitro dissolution time. The tablets disintegrated within 21 to 75 s. Almost 95% of drug was released from all formulations within 15 min. The formulation containing 6% of croscarmellose sodium (F6) was found to give the best results. Apart from fulfilling all official and other specifications, the tablets exhibited higher rate of release. The stability studies were performed as per ICH guidelines. The Optimized formulation (F6) showed no significant variations for the tablets parameters and it was stable for the specified time period. It was concluded the Orodispersible tablets for Rizatriptan Benzoate can be formulated for emergency treatment of migraine.","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"13 1","pages":"186-193"},"PeriodicalIF":0.0,"publicationDate":"2011-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75349512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Good quality research requires thoughtful preparation prior to undertaking data collection and analysis. Successful researchers use their ideas as the basis for conceptualization of the study. The research purpose statement, coming from a literature-based conceptualization, indicates the most appropriate research design and leads the researcher toward a successful completion of the study.
{"title":"Getting Started with Research: Ideas to Research Process","authors":"C. Bartz","doi":"10.5530/RJPS.2011.3.1","DOIUrl":"https://doi.org/10.5530/RJPS.2011.3.1","url":null,"abstract":"Good quality research requires thoughtful preparation prior to undertaking data collection and analysis. Successful researchers use their ideas as the basis for conceptualization of the study. The research purpose statement, coming from a literature-based conceptualization, indicates the most appropriate research design and leads the researcher toward a successful completion of the study.","PeriodicalId":21459,"journal":{"name":"RGUHS Journal of Pharmaceutical Sciences","volume":"35 1","pages":"176-179"},"PeriodicalIF":0.0,"publicationDate":"2011-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88662129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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