Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1969.TB01954.X
H. Day, H. Holmsen, T. Hovig
Human platelet subcellular particles have been fractionated by three methods. The isolated fractions have been examined by electron microscopy and assayed for protein, serotonin, lactate dehydrogenase, cytochrome c oxidase, β-hydroxybutyrate dehydrogenase, 5′-nucleotidase, acid phosphatase, β-glucu-ronidase, β-galactosidase, β-N-acetylgluco-saminidase, cathepsin, aryl sulphatase and a-mannosidase. The latter two enzymes had low activities. Homogenization with blendor caused great solubilization of serotonin and lysosomal enzymes but not cytochrome c oxidase. The granules in the homogenate were swollen and showed great loss of osmo-philia. The particles were isolated by differential centrifugation and separated into 3 zones after centrifugation on discontinuous sucrose gradients. The upper zone contained proteinaceous material, the second contained mostly vesicular membranes and the lowest one contained α-granules, transected pseudo-pods plus mitochondria with varying degrees of preservation. Acid phosphatase was present in the membranes but the highest activity was found in the upper part of the granular band together with β-galactosidase. β-Glucuronidase, β-N-acetylglucosaminidase and cathepsin were mainly located in the lower part of this band. Homogenization with a ‘no-clearance’ Teflon pestle caused less solubilization than the blendor and gave better organelle preservation. Isolated particles from such homogenates separated into two bands on gradient centrifugation which morphologically and biochemically were equal to those obtained when these homogenates were applied directly on the gradient. The upper particulate band contained membranes and had bound 5′-nucleotidase. The lower band possessed in its upper part well-preserved α-granules and mitochondria whereas in the lower regions more electron dense granules, often with ‘Bull's eye’, were present. Swollen α-granules were present mainly in the lower part of this band. All lysosomal enzymes were localized at the same sucrose density. Probably, the α-gra-nules are the platelet lysosomes, but with pestle homogenization there was not the biochemical heterogenity as found when using the blendor. With pestle homogenization particle bound serotonin was found in fractions of high sucrose density which contained the dense α-granules with the ‘Bull's eye’.
人血小板亚细胞颗粒已被三种方法分离。对分离的部分进行了电镜检查，并测定了蛋白质、血清素、乳酸脱氢酶、细胞色素c氧化酶、β-羟基丁酸脱氢酶、5′-核苷酸酶、酸性磷酸酶、β-葡糖苷酶、β-半乳糖苷酶、β- n -乙酰葡萄糖胺苷酶、组织蛋白酶、芳基硫酸酯酶和a-甘露糖苷酶的含量。后两种酶的活性较低。用混合液均质可使血清素和溶酶体酶溶解，但细胞色素c氧化酶不溶解。匀浆中的颗粒肿胀，亲渗透性明显丧失。采用差速离心分离颗粒，在不连续的蔗糖梯度上离心后将颗粒分离成3个区。上部区主要为蛋白质物质，下部区主要为泡膜，下部区主要为α-颗粒、横切的伪豆荚和保存程度不同的线粒体。酸性磷酸酶存在于细胞膜中，但与β-半乳糖苷酶一起在颗粒带的上部活性最高。β-葡萄糖醛酸酶、β- n -乙酰氨基葡萄糖醛酸酶和组织蛋白酶主要位于该带的下半部分。使用“无间隙”特氟龙杵进行均质处理，其增溶作用比混合剂小，并能更好地保存细胞器。从这种匀浆中分离出来的颗粒在梯度离心作用下被分离成两个条带，其形态和生物化学性质与这些匀浆直接应用于梯度时得到的结果相同。上部颗粒带含有膜并结合了5′-核苷酸酶。下带上部有保存完好的α-颗粒和线粒体，而下带较多的电子致密颗粒，常呈“牛眼”状。肿胀的α-颗粒主要出现在该带的下部。所有溶酶体酶都定位在相同的蔗糖密度上。α-粒核可能是血小板溶酶体，但经杵均质处理后，其生化异质性不像使用混合剂时那样明显。用杵均质法在含有致密α-颗粒的高蔗糖密度组分中发现了颗粒结合的血清素。
{"title":"Subcellular Particles of Human Platelets","authors":"H. Day, H. Holmsen, T. Hovig","doi":"10.1111/J.1600-0609.1969.TB01954.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1969.TB01954.X","url":null,"abstract":"Human platelet subcellular particles have been fractionated by three methods. The isolated fractions have been examined by electron microscopy and assayed for protein, serotonin, lactate dehydrogenase, cytochrome c oxidase, β-hydroxybutyrate dehydrogenase, 5′-nucleotidase, acid phosphatase, β-glucu-ronidase, β-galactosidase, β-N-acetylgluco-saminidase, cathepsin, aryl sulphatase and a-mannosidase. The latter two enzymes had low activities. Homogenization with blendor caused great solubilization of serotonin and lysosomal enzymes but not cytochrome c oxidase. The granules in the homogenate were swollen and showed great loss of osmo-philia. The particles were isolated by differential centrifugation and separated into 3 zones after centrifugation on discontinuous sucrose gradients. The upper zone contained proteinaceous material, the second contained mostly vesicular membranes and the lowest one contained α-granules, transected pseudo-pods plus mitochondria with varying degrees of preservation. Acid phosphatase was present in the membranes but the highest activity was found in the upper part of the granular band together with β-galactosidase. β-Glucuronidase, β-N-acetylglucosaminidase and cathepsin were mainly located in the lower part of this band. Homogenization with a ‘no-clearance’ Teflon pestle caused less solubilization than the blendor and gave better organelle preservation. Isolated particles from such homogenates separated into two bands on gradient centrifugation which morphologically and biochemically were equal to those obtained when these homogenates were applied directly on the gradient. The upper particulate band contained membranes and had bound 5′-nucleotidase. The lower band possessed in its upper part well-preserved α-granules and mitochondria whereas in the lower regions more electron dense granules, often with ‘Bull's eye’, were present. Swollen α-granules were present mainly in the lower part of this band. All lysosomal enzymes were localized at the same sucrose density. Probably, the α-gra-nules are the platelet lysosomes, but with pestle homogenization there was not the biochemical heterogenity as found when using the blendor. With pestle homogenization particle bound serotonin was found in fractions of high sucrose density which contained the dense α-granules with the ‘Bull's eye’.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"4 1","pages":"3-35"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79117967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1986.TB01748.X
Y. Najean, refractory anaemias
A prospective randomized study of androgen therapy in aplastic anaemia (AA) was performed: 2 androgens (fluoxymesterone and norethandrolone) at high (1 mg/kg/d) and low (0.2 mg/kg/d) dose were studied on 110 patients; and 4 androgens given at high doses were objectively compared in 125 other cases. When patients are matched for AA severity there is an obvious efficiency of the high-dose androgens in the survival of the less severe cases of aplastic anaemia and in their haematological improvement. The data further show that, among the 4 androgens tested, fluoxymesterone is the most efficient and stanozolol the least. We conclude that androgen therapy is truly efficient in moderately severe aplastic anaemia and that fluoxymesterone (1 mg/kg/d) for 18 months should be chosen as reference drug to any new androgen therapy assay.
{"title":"Androgen therapy in aplastic anaemia: a comparative study of high and low-doses and of 4 different androgens. French Cooperative Group for the Study of Aplastic and Refractory Anemias.","authors":"Y. Najean, refractory anaemias","doi":"10.1111/J.1600-0609.1986.TB01748.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1986.TB01748.X","url":null,"abstract":"A prospective randomized study of androgen therapy in aplastic anaemia (AA) was performed: 2 androgens (fluoxymesterone and norethandrolone) at high (1 mg/kg/d) and low (0.2 mg/kg/d) dose were studied on 110 patients; and 4 androgens given at high doses were objectively compared in 125 other cases. When patients are matched for AA severity there is an obvious efficiency of the high-dose androgens in the survival of the less severe cases of aplastic anaemia and in their haematological improvement. The data further show that, among the 4 androgens tested, fluoxymesterone is the most efficient and stanozolol the least. We conclude that androgen therapy is truly efficient in moderately severe aplastic anaemia and that fluoxymesterone (1 mg/kg/d) for 18 months should be chosen as reference drug to any new androgen therapy assay.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"51 1","pages":"346-52"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76836127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1981.TB01409.X
G. Sébahoun, J. Bayle, R. Muratore, Y. Carcassonne
A case of acquired dyserythropoiesis with inter-erythroblastic connections is reported in a patient with chronic myeloid leukaemia, developing a terminal acute hepatic failure related to hepatocarcinoma. The erythroblastic series was abundant but only made of clusters grouping 10 to 20 closely adherent cells. The cellular membranes showed linear junctions or were interdigitated and the intercellular space was occupied with electron dense ferritin granules. This non specific aspect of dyserythropoiesis may be related to the hepatic carcinoma, which was probably induced by busulfan therapy.
{"title":"Acquired dyserythropoiesis with abnormal intercellular contacts between erythroblasts. Report of a patient with chronic myeloid leukaemia and hepatocarcinoma.","authors":"G. Sébahoun, J. Bayle, R. Muratore, Y. Carcassonne","doi":"10.1111/J.1600-0609.1981.TB01409.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1981.TB01409.X","url":null,"abstract":"A case of acquired dyserythropoiesis with inter-erythroblastic connections is reported in a patient with chronic myeloid leukaemia, developing a terminal acute hepatic failure related to hepatocarcinoma. The erythroblastic series was abundant but only made of clusters grouping 10 to 20 closely adherent cells. The cellular membranes showed linear junctions or were interdigitated and the intercellular space was occupied with electron dense ferritin granules. This non specific aspect of dyserythropoiesis may be related to the hepatic carcinoma, which was probably induced by busulfan therapy.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"91 1","pages":"318-22"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75082237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1969.TB02409.X
D. Roelcke, G. Uhlenbruck, K. Bauer
The HD receptors of human erythrocytes, which are demonstrable by HD cold auto antibodies and can be inactivated by both neuraminidase and proteases, are heterogenous. Whereas the HD1 receptor was found exclusively on human erythrocytes, the HD2 receptor could be demonstrated on human as well as on rat and guinea-pig erythrocytes. As neuraminidase has been shown to be the ‘specific’ enzyme for inactivating both the HD1 and HD2 receptors it can be concluded that also the configuration of the neuraminic acid as the HD determinant must be different. The special position of the HD receptor complex in comparison to other receptors determined by neuraminic acid is stressed.
{"title":"A heterogeneity of the HD-receptor, demonstrable by HD-cold antibodies: HD1-HD2-immunochemical aspects.","authors":"D. Roelcke, G. Uhlenbruck, K. Bauer","doi":"10.1111/J.1600-0609.1969.TB02409.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1969.TB02409.X","url":null,"abstract":"The HD receptors of human erythrocytes, which are demonstrable by HD cold auto antibodies and can be inactivated by both neuraminidase and proteases, are heterogenous. Whereas the HD1 receptor was found exclusively on human erythrocytes, the HD2 receptor could be demonstrated on human as well as on rat and guinea-pig erythrocytes. As neuraminidase has been shown to be the ‘specific’ enzyme for inactivating both the HD1 and HD2 receptors it can be concluded that also the configuration of the neuraminic acid as the HD determinant must be different. The special position of the HD receptor complex in comparison to other receptors determined by neuraminic acid is stressed.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"38 1","pages":"280-7"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75808135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1968.TB01716.X
R. Grahl-Madsen, G. Pallisgaard
Acute renal failure is reported during a haemolytic crisis in a boy with hereditary spherocytosis since this complication has not previously been published.
急性肾功能衰竭的报告在溶血危机的遗传性球形红细胞增多症的男孩，因为这种并发症以前没有发表。
{"title":"Acute Renal Failure in Hereditary Spherocytosis. Report of a Case","authors":"R. Grahl-Madsen, G. Pallisgaard","doi":"10.1111/J.1600-0609.1968.TB01716.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1968.TB01716.X","url":null,"abstract":"Acute renal failure is reported during a haemolytic crisis in a boy with hereditary spherocytosis since this complication has not previously been published.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"12 1","pages":"41-46"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72839358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1974.TB00179.X
Bengt Björksténm
The results of NBT tests were found to vary depending on how the tests were performed. Heparin increased the proportion of NBT positive neutrophils and the lowest possible concentration should be used. Blood samples for the NBT test could be stored for 8 h at 4° C with only a minor effect on the test results. The incubation time was crucial and 25 min in a 37° C water bath was adopted as standard procedure.
{"title":"The Influence of Technical Factors on the NBT Test","authors":"Bengt Björksténm","doi":"10.1111/J.1600-0609.1974.TB00179.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1974.TB00179.X","url":null,"abstract":"The results of NBT tests were found to vary depending on how the tests were performed. Heparin increased the proportion of NBT positive neutrophils and the lowest possible concentration should be used. Blood samples for the NBT test could be stored for 8 h at 4° C with only a minor effect on the test results. The incubation time was crucial and 25 min in a 37° C water bath was adopted as standard procedure.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"55 1","pages":"46-50"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74120970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1984.TB00686.X
B. Østerud
{"title":"Activation pathways of the coagulation system in normal haemostasis.","authors":"B. Østerud","doi":"10.1111/J.1600-0609.1984.TB00686.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1984.TB00686.X","url":null,"abstract":"","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"22 1","pages":"337-345"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90869599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1983.TB02129.X
P. Hörnsten, B. Sundman-Engberg, G. Gahrton, B. Johansson
An overdose of CCNU (600 mg over a 15-d period) was unintentionally ingested by a patient with advanced Hodgkin's disease subjected to combination chemotherapy. A severe bone marrow depression occurred 3 weeks after the start of the CCNU treatment. The nadir of the platelet count was reached after 4 weeks and that of the granulocyte count after 5 weeks. At the nadir of the white blood cell count, colony-forming cells (CFU-C) were found in significantly reduced numbers in the bone marrow, and were not found at all in the peripheral blood; the amount of colony-stimulating activity (CSA) produced by peripheral blood cells was reduced. However, the cells producing CSA recovered earlier than the CFU-C, and the CSA peak value was reached about 1 week before the peak value for CFU-C in the bone marrow. Thus, in vivo CSA-producing cells appeared to be more resistant to CCNU than were CFU-C, and their recovery appeared to be a prerequisite for the recovery of CFU-C and myelopoietic cells.
{"title":"CCNU toxicity after an overdose in a patient with Hodgkin's disease. Effects on colony-forming cells (CFU-C) and colony-stimulating activity (CSA).","authors":"P. Hörnsten, B. Sundman-Engberg, G. Gahrton, B. Johansson","doi":"10.1111/J.1600-0609.1983.TB02129.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1983.TB02129.X","url":null,"abstract":"An overdose of CCNU (600 mg over a 15-d period) was unintentionally ingested by a patient with advanced Hodgkin's disease subjected to combination chemotherapy. A severe bone marrow depression occurred 3 weeks after the start of the CCNU treatment. The nadir of the platelet count was reached after 4 weeks and that of the granulocyte count after 5 weeks. At the nadir of the white blood cell count, colony-forming cells (CFU-C) were found in significantly reduced numbers in the bone marrow, and were not found at all in the peripheral blood; the amount of colony-stimulating activity (CSA) produced by peripheral blood cells was reduced. However, the cells producing CSA recovered earlier than the CFU-C, and the CSA peak value was reached about 1 week before the peak value for CFU-C in the bone marrow. Thus, in vivo CSA-producing cells appeared to be more resistant to CCNU than were CFU-C, and their recovery appeared to be a prerequisite for the recovery of CFU-C and myelopoietic cells.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"26 1","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84712807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1969.TB01820.X
Helleman Pw
{"title":"The Toa micro cell counter. 3. A comparative study of the results of erythrocyte and leucocyte counts with the Toa micro cell counter and with the coulter counter.","authors":"Helleman Pw","doi":"10.1111/J.1600-0609.1969.TB01820.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1969.TB01820.X","url":null,"abstract":"","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"25 1","pages":"160-165"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82214397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1969.TB02411.X
M. Claesson, P. Høyer, C. Ropke
Procedures are presented which will make it possible to fix lymphoid cells exposed to the Dye Exclusion Test without influencing the percentage of dye-absorbing (dead or dying) cells.
{"title":"Permanent fixation of lymphoid cells exposed to dye exclusion test.","authors":"M. Claesson, P. Høyer, C. Ropke","doi":"10.1111/J.1600-0609.1969.TB02411.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1969.TB02411.X","url":null,"abstract":"Procedures are presented which will make it possible to fix lymphoid cells exposed to the Dye Exclusion Test without influencing the percentage of dye-absorbing (dead or dying) cells.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"54 6","pages":"291-2"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72630921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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