Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1975.TB02709.X
Z. Ben-ishay
Ultrastructural observations of erythroid colonies in diffusion chambers implanted in lethally irradiated rats are reported. The donor cells were obtained from rats treated with cytosine arabinoside (250 mg/kg body weight) 6 h prior to seeding. The marrow of donor rats contained a high proportion of stem cells committed to the erythroid line. 9 days after implantation, vast numbers of erythroid colonies developed in the diffusion chambers. The erythroid cells were observed arranged around a central macrophage resembling erythroid islands in bone marrow. Erythroid colonies most probably differentiated from committed stem cells in the inoculum. It is postulated that development of erythroid colonies in diffusion chambers is conditioned by, among other factors, the presence of a special type of macrophages. These macrophages have the same function as those of central reticular cells (CRC) in bone marrow erythroid islands.
{"title":"Ultrastructural analysis of erythroid colonies in diffusion chambers in irradiated rats. Effect of cytosine arabinoside administration on donor rats.","authors":"Z. Ben-ishay","doi":"10.1111/J.1600-0609.1975.TB02709.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1975.TB02709.X","url":null,"abstract":"Ultrastructural observations of erythroid colonies in diffusion chambers implanted in lethally irradiated rats are reported. The donor cells were obtained from rats treated with cytosine arabinoside (250 mg/kg body weight) 6 h prior to seeding. The marrow of donor rats contained a high proportion of stem cells committed to the erythroid line. 9 days after implantation, vast numbers of erythroid colonies developed in the diffusion chambers. The erythroid cells were observed arranged around a central macrophage resembling erythroid islands in bone marrow. Erythroid colonies most probably differentiated from committed stem cells in the inoculum. It is postulated that development of erythroid colonies in diffusion chambers is conditioned by, among other factors, the presence of a special type of macrophages. These macrophages have the same function as those of central reticular cells (CRC) in bone marrow erythroid islands.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"10 1","pages":"369-76"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79891187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1967.TB01649.X
C. Wasastjerna, H. Kalliola, J. Räsänen, O. Wager
{"title":"IgG cryoglobulinaemia Case report and immunological studies of a patient with recurrent ulcerative stomatitis and high content of cold precipitable immunoglobulin in serum.","authors":"C. Wasastjerna, H. Kalliola, J. Räsänen, O. Wager","doi":"10.1111/J.1600-0609.1967.TB01649.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1967.TB01649.X","url":null,"abstract":"","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"48 1","pages":"473-84"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80251826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1970.TB01862.X
M. Fagerhol, U. Abildgaard
Isolation of what is probably the main thrombin inhibitor antithrombin III (At-III) made the preparation of specific antisera for immunochemical analysis possible. When immunoelectrophoresis of At III against anti At III and anti whole human serum was performed a single precipitation arc was formed in the alpha 2-globulin region. When thrombin was added the apparent concentration of At III decreased. Serum concentration of At III is probably influenced by sex hormones since women of childbearing age have lower serum concentrations of At III. They are further decreased by oral contraceptive implying a relationship between the low At III levels and the tendency to thrombosis in women using oral contraceptives.
{"title":"Immunological studies on human antithrombin 3. Influence of age, sex and use of oral contraceptives on serum concentration.","authors":"M. Fagerhol, U. Abildgaard","doi":"10.1111/J.1600-0609.1970.TB01862.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1970.TB01862.X","url":null,"abstract":"Isolation of what is probably the main thrombin inhibitor antithrombin III (At-III) made the preparation of specific antisera for immunochemical analysis possible. When immunoelectrophoresis of At III against anti At III and anti whole human serum was performed a single precipitation arc was formed in the alpha 2-globulin region. When thrombin was added the apparent concentration of At III decreased. Serum concentration of At III is probably influenced by sex hormones since women of childbearing age have lower serum concentrations of At III. They are further decreased by oral contraceptive implying a relationship between the low At III levels and the tendency to thrombosis in women using oral contraceptives.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"49 1","pages":"10-7"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75141961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1979.TB02863.X
B. Lau, G. Jäger, E. Thiel, H. Rodt, D. Huhn, K. Pachmann, B. Netzel, L. Böning, S. Thierfelder, P. Dörmer
Cells of the Reh line, originally derived from an ALL of the 'Non-T, Non-B' type were cultured in diffusion chambers implanted intraperitoneally into perirradiated CBA mice. At different intervals over a period of 20 d changes in surface characteristics were examined by labelling the cells with AcALLG, ATCG as well as with polyvalent AIg. The evaluation was performed by using direct immunofluorescence. In addition, the ability to form rosetts with SRBC, AET-treated sheep erythrocytes, mouse red blood cells and EoxAC was tested. On day 0 of the diffusion chamber culture the cells only carried cALLA, and no rosette formation was observed. In the course of the diffusion chamber culture the cells unequivocally developed T-cell antigen, and in 1 of 2 experiments they further acquired a receptor for forming AET- and E-rosettes. Conversely, in the other experiment a receptor for mouse red blood cells was detected in a considerable portion of the cells. Our data show that the rather undifferentiated Reh line cells in vitro are able to develop features of mature T-cells and attributes of early B-cells during the diffusion chamber culture. In vitro they apparently retain a bivalent potentiality of lymphatic maturation. The diffusion chamber system proves to be a suitable tool for promoting differentiation in these cells.
{"title":"Growth of the Reh cell line in diffusion chambers. Evidence for differentiation along the T- and B-cell pathway.","authors":"B. Lau, G. Jäger, E. Thiel, H. Rodt, D. Huhn, K. Pachmann, B. Netzel, L. Böning, S. Thierfelder, P. Dörmer","doi":"10.1111/J.1600-0609.1979.TB02863.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1979.TB02863.X","url":null,"abstract":"Cells of the Reh line, originally derived from an ALL of the 'Non-T, Non-B' type were cultured in diffusion chambers implanted intraperitoneally into perirradiated CBA mice. At different intervals over a period of 20 d changes in surface characteristics were examined by labelling the cells with AcALLG, ATCG as well as with polyvalent AIg. The evaluation was performed by using direct immunofluorescence. In addition, the ability to form rosetts with SRBC, AET-treated sheep erythrocytes, mouse red blood cells and EoxAC was tested. On day 0 of the diffusion chamber culture the cells only carried cALLA, and no rosette formation was observed. In the course of the diffusion chamber culture the cells unequivocally developed T-cell antigen, and in 1 of 2 experiments they further acquired a receptor for forming AET- and E-rosettes. Conversely, in the other experiment a receptor for mouse red blood cells was detected in a considerable portion of the cells. Our data show that the rather undifferentiated Reh line cells in vitro are able to develop features of mature T-cells and attributes of early B-cells during the diffusion chamber culture. In vitro they apparently retain a bivalent potentiality of lymphatic maturation. The diffusion chamber system proves to be a suitable tool for promoting differentiation in these cells.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"72 1","pages":"385-92"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75102772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1976.TB01123.X
R. Hast, K. Skårberg, L. Engstedt, S. Jameson, A. Killander, B. Lundh, P. Reizenstein, A. Udén, B. Wadman
This is a prospective multi-center study in which patients with aregenerative anaemia were treated with a standardized high dosage regime of an anabolic steroid (oxymetholone, Anasteron). 53 patients were included and divided into two groups according to bone marrow cellularity. Furthermore the hypocellular group was subdivided in order to make comparison with earlier studies possible. In the hypocellular group, the frequency of remission was 56% and the 2-year-survival from the onset of symptoms was 75%. This is longer than in some earlier studies, perhaps because of possible differences in etiology and/or because of the effect of systematic high dosage, long term androgen therapy. Patient selection was minimized and was not considered to be of major importance. Patients with hypercellular marrows, on the other hand, responded poorly to androgens. In this group 63% died of acute leukaemia, which confirms earlier suggestions that this form of aregenerative anaemia, frequently is of a preleukaemic nature.
{"title":"Oxymetholone treatment in aregenerative anaemia. II. Remission and survival--a prospective study.","authors":"R. Hast, K. Skårberg, L. Engstedt, S. Jameson, A. Killander, B. Lundh, P. Reizenstein, A. Udén, B. Wadman","doi":"10.1111/J.1600-0609.1976.TB01123.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1976.TB01123.X","url":null,"abstract":"This is a prospective multi-center study in which patients with aregenerative anaemia were treated with a standardized high dosage regime of an anabolic steroid (oxymetholone, Anasteron). 53 patients were included and divided into two groups according to bone marrow cellularity. Furthermore the hypocellular group was subdivided in order to make comparison with earlier studies possible. In the hypocellular group, the frequency of remission was 56% and the 2-year-survival from the onset of symptoms was 75%. This is longer than in some earlier studies, perhaps because of possible differences in etiology and/or because of the effect of systematic high dosage, long term androgen therapy. Patient selection was minimized and was not considered to be of major importance. Patients with hypercellular marrows, on the other hand, responded poorly to androgens. In this group 63% died of acute leukaemia, which confirms earlier suggestions that this form of aregenerative anaemia, frequently is of a preleukaemic nature.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"53 44 1","pages":"90-100"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80484680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1967.TB01646.X
G. Myllylä, R. Pelkonen, E. Ikkala, J. Apajalahti
{"title":"Hereditary thrombocytopenia. Report of three families.","authors":"G. Myllylä, R. Pelkonen, E. Ikkala, J. Apajalahti","doi":"10.1111/J.1600-0609.1967.TB01646.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1967.TB01646.X","url":null,"abstract":"","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"9 1","pages":"441-452"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82415468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1983.TB01518.X
T. W. Hansen, M. Seip, C. D. de Verdier, Å. Ericson
We present 2 Norwegian siblings who we believe to be the first cases of pyrimidine 5'-nucleotidase deficiency diagnosed in the Scandinavian countries. In addition to the features presented by previously published cases, our patients exhibited signs of intravascular haemolysis with haemoglobinuria and increased loss of iron in the urine. The loss of iron has made iron medication necessary from time to time. A kidney biopsy from the oldest patient revealed accumulation of iron in the epithelium of the proximal tubuli. The kidneys were considerably enlarged in both patients, but with normal function tests.
{"title":"Erythrocyte pyrimidine 5'-nucleotidase deficiency. Report of 2 new cases, with a review of the literature.","authors":"T. W. Hansen, M. Seip, C. D. de Verdier, Å. Ericson","doi":"10.1111/J.1600-0609.1983.TB01518.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1983.TB01518.X","url":null,"abstract":"We present 2 Norwegian siblings who we believe to be the first cases of pyrimidine 5'-nucleotidase deficiency diagnosed in the Scandinavian countries. In addition to the features presented by previously published cases, our patients exhibited signs of intravascular haemolysis with haemoglobinuria and increased loss of iron in the urine. The loss of iron has made iron medication necessary from time to time. A kidney biopsy from the oldest patient revealed accumulation of iron in the epithelium of the proximal tubuli. The kidneys were considerably enlarged in both patients, but with normal function tests.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"29 1","pages":"122-8"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86200193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1981.TB01367.X
K. Kragballe, J. Ellegaard, T. Herlin
Monocyte mediated lysis of human type B-erythrocytes coated with heat-inactivated anti-B serum was measured as 51Cr-release. Preincubation of monocytes with heat-inactivated normal AB serum inhibited cytolysis while a strong stimulation of cytolysis was seen with fresh AB serum. These effects of serum were reversible upon cell washing. Preincubation of monocytes with hyperimmune human serum against type B erythrocytes did not alter the cytolysis. After incubation at 37 degrees C of monocytes with immune complexes formed of human albumin and rabbit anti-human albumin a dose-related inhibition of cytolysis was observed. This inhibition was equally expressed at different albumin to anti-albumin ratios. Pretreatment with immune complexes at 4 degrees C had the same effect on cytolysis as pretreatment at 37 degrees C. The effect of immune complexes on monocyte-mediated cytolysis was resistant to repeated cell washing. After incubation of pretreated monocytes at 37 degrees C for up to 18 h some of the inhibition disappeared, but without normalization. Platelet depletion of monocyte suspensions resulted in a significant increase of cytolysis, and addition of washed platelets reinduced a decreased of cytolysis, which correlated with the number of platelets added.
{"title":"Antibody-dependent monocyte-mediated cytotoxicity. The interference by platelets, immune complexes, and normal serum.","authors":"K. Kragballe, J. Ellegaard, T. Herlin","doi":"10.1111/J.1600-0609.1981.TB01367.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1981.TB01367.X","url":null,"abstract":"Monocyte mediated lysis of human type B-erythrocytes coated with heat-inactivated anti-B serum was measured as 51Cr-release. Preincubation of monocytes with heat-inactivated normal AB serum inhibited cytolysis while a strong stimulation of cytolysis was seen with fresh AB serum. These effects of serum were reversible upon cell washing. Preincubation of monocytes with hyperimmune human serum against type B erythrocytes did not alter the cytolysis. After incubation at 37 degrees C of monocytes with immune complexes formed of human albumin and rabbit anti-human albumin a dose-related inhibition of cytolysis was observed. This inhibition was equally expressed at different albumin to anti-albumin ratios. Pretreatment with immune complexes at 4 degrees C had the same effect on cytolysis as pretreatment at 37 degrees C. The effect of immune complexes on monocyte-mediated cytolysis was resistant to repeated cell washing. After incubation of pretreated monocytes at 37 degrees C for up to 18 h some of the inhibition disappeared, but without normalization. Platelet depletion of monocyte suspensions resulted in a significant increase of cytolysis, and addition of washed platelets reinduced a decreased of cytolysis, which correlated with the number of platelets added.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"1 1","pages":"67-75"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83125010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-04-24DOI: 10.1111/J.1600-0609.1983.TB01462.X
A. Polliack, R. Leizerowitz, A. Berrebi, D. Gurfel, H. Gamliel
Megakaryoblasts and maturing megakaryocytic precursor cells from 5 patients with megakaryoblastic leukaemia were studied by scanning electron microscopy (SEM). The diagnosis in all cases had been established by ultrastructural cytochemistry on the basis of a positive platelet peroxidase reaction with negative staining for myeloperoxidase. 1 case presented as acute myelofibrosis and 4 as acute megakaryoblastic transformation of chronic granulocytic leukaemia. Under the SEM, megakaryoblasts and maturing megakaryocytic precursors showed typical surface features including the presence of rounded and irregular blebs, broad folds and pseudopodia. The nature of these surface blebs is still unclear but they probably represent surface membrane alterations relating to imminent platelet shedding at least in the more mature precursors. These surface microprojections are distinctly different from those encountered on leukaemic lymphoblasts, myeloblasts and monoblasts. It is suggested that SEM may be used in conjunction with the PPO reaction as in aid in the diagnosis of megakaryocytic leukaemias.
{"title":"Surface features of leukaemic megakaryocytic precursors. A study of 5 cases of megakaryoblastic leukaemia with scanning electron microscopy.","authors":"A. Polliack, R. Leizerowitz, A. Berrebi, D. Gurfel, H. Gamliel","doi":"10.1111/J.1600-0609.1983.TB01462.X","DOIUrl":"https://doi.org/10.1111/J.1600-0609.1983.TB01462.X","url":null,"abstract":"Megakaryoblasts and maturing megakaryocytic precursor cells from 5 patients with megakaryoblastic leukaemia were studied by scanning electron microscopy (SEM). The diagnosis in all cases had been established by ultrastructural cytochemistry on the basis of a positive platelet peroxidase reaction with negative staining for myeloperoxidase. 1 case presented as acute myelofibrosis and 4 as acute megakaryoblastic transformation of chronic granulocytic leukaemia. Under the SEM, megakaryoblasts and maturing megakaryocytic precursors showed typical surface features including the presence of rounded and irregular blebs, broad folds and pseudopodia. The nature of these surface blebs is still unclear but they probably represent surface membrane alterations relating to imminent platelet shedding at least in the more mature precursors. These surface microprojections are distinctly different from those encountered on leukaemic lymphoblasts, myeloblasts and monoblasts. It is suggested that SEM may be used in conjunction with the PPO reaction as in aid in the diagnosis of megakaryocytic leukaemias.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"57 1","pages":"145-50"},"PeriodicalIF":0.0,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86792250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Elevation of PAIgG on intact and lysed platelets in the case unresponsive to intravenous gammaglobulin.","authors":"S Ozsoylu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"37 5","pages":"456"},"PeriodicalIF":0.0,"publicationDate":"1986-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13581245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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