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Photoperiodic-dependent morpho-biometric changes in the excurrent duct system of sexually-matured helmeted guinea fowl (Numida meleagris) 性成熟珍珠鸡排泄系统的光周期依赖性形态生物特征变化
Pub Date : 2022-09-30 DOI: 10.54058/saheljvs.v19i3.320
A. Baso, M. B. Umar, M. H. Sulaiman, I. J. Gosomji, J. Omirinde, T. Muazu, M. Zubairu, S. Atabo
Photoperiod is considered to be one of the most effective environmental factor in controlling the reproductive cycle and gonadal maturation in some mammalian species, but very limited information are available on its effects on  reproductive biology of the guinea fowl.  Therein, we  investigated the effects of varying photoperiodic treatments on morpho-biometry of the excurrent duct system of helmeted guinea fowl (Numida meleagris).  A total of twenty-one (21) apparently healthy sexually-matured helmeted guinea fowls were randomly assigned into three (3) photoperiodic regimes; mainly [Group I: Short daylight (SD; 8 HL (Hour of light, Group II: Moderate daylight (MD; 12 HL) and Group III: Long daylight (LD; 16 HL)] of seven birds per group (n=7). After 8 weeks of experimentation, the excurrent ducts were excised and freed of all adhering connective tissues for gross-morphological and morphometric evaluations. The bio-morphometric data (weight and diameter) were significantly increased (p< 0.05) in the epididymis and ductus deferens of guinea fowl exposed to 16 HL, as compared to other treatments. However, no significant (p> 0.05) effect was observed in body weight and length of the excurrent ducts regardless of photoperiod.    The observed changes in weight and diameter of the epididymis and ductus deferens of helmeted guinea fowl are thus a consequence of the stimulatory effects of long photoperiod exposure to reproductive activity. Thus, these findings would therefore be useful to guinea fowl breeders in designing appropriate intervention programs necessary to improve their  reproduction efficacy and to preserve this species.  
光周期被认为是控制某些哺乳动物生殖周期和性腺成熟的最有效的环境因素之一,但关于光周期对珍珠鸡生殖生物学影响的研究非常有限。在此基础上,我们研究了不同光周期处理对头盔鸡排泄管系统形态生物统计学的影响。21只性成熟的健康头套珍珠鸡被随机分为3个光周期组;主要是[第一组:短日照(SD;8 HL(光照小时,第二组:中度日光(MD;12 HL)及第三组:长日光(LD);16 HL)]每组7只(n=7)。实验8周后,切除出管,去除所有粘连结缔组织,进行大体形态学和形态计量学评估。与其他处理相比,16 HL处理的雏鸡附睾和输精管的生物形态学数据(重量和直径)显著增加(p< 0.05)。但不同光周期对大鼠体质量和出流管长度均无显著影响(p> 0.05)。因此,所观察到的戴头盔的珍珠鸡附睾和管的重量和直径的变化是长时间暴露于生殖活动的光周期刺激作用的结果。因此,这些发现将有助于珍珠鸡养殖者设计适当的干预方案,以提高其繁殖效率并保护该物种。
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引用次数: 1
Reproductive Tract Morphometry and Evaluation of Fresh Wild African ‎Catfish (Clarias gariepinus) Milt from Lake Alau, Maiduguri, Nigeria‎ 尼日利亚迈杜古里Alau湖新鲜野生非洲鲇鱼(Clarias gariepinus) Milt生殖道形态测定及评价
Pub Date : 2022-09-29 DOI: 10.54058/saheljvs.v19i3.312
R. Ali, S. Asuku, U. Bamanga, A. Ali, M. Waziri, M. M. Bukar
Reproductive morphometry of Clarias gariepinus (C. gariepinus) is essential in distinguishing different species that are closely related to it and adequate information is required regarding the seasonal variation of milt characteristic of wild species. This study was designed to document the reproductive tract morphometric dimensions and milt characteristics of wild C. gariepinus during spawning and non-spawning seasons in Lake Alau, Maiduguri, Nigeria. Sixty male brood stocks were used comprising thirty each for spawning and non-spawning seasons. The reproductive tracts were removed and their dimensions measured. The milt samples were collected, pooled and evaluated. Results of the study showed that the body weight, sperm concentration and testicular indices differed significantly (p˂0.05) between the two seasons of evaluation. In addition, a positive correlation (p˂ 0.05) between body weight of fish and milt volume (r = 0.656 and r = 0.646) was also observed in both seasons. It was concluded that the left testis of C. gariepinus is heavier, longer and thicker than the right. Nonetheless, the C. gariepinus had larger body weights and higher spermatozoa concentration in spawning season than in non-spawning season; those with larger body weights had higher volume of milt than those with lower body weights.
加里平瓢虫(Clarias gariepinus, C. gariepinus)的生殖形态测定是区分其近缘种的重要依据,对野生种的生殖形态特征的季节变化需要有充分的了解。本研究旨在记录尼日利亚迈杜古里阿劳湖产卵季节和非产卵季节野生加里滨棘鱼(C. gariepinus)生殖道的形态尺寸和形态特征。共使用60个雄性育雏群,其中产卵季节和非产卵季节各30个。切除生殖道并测量其尺寸。这些样本被收集、汇总和评估。研究结果表明,在两个评估季节之间,体重、精子浓度和睾丸指数差异显著(p小于0.05)。此外,在两个季节中,鱼的体重与鱼体体积之间也观察到正相关(p小于0.05)(r = 0.656和r = 0.646)。结果表明:左睾丸比右睾丸重、长、粗。产卵季节的鱼体质量和精子浓度均高于非产卵季节;体重大的人比体重小的人有更高的肌肉体积。
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引用次数: 0
Influence of Age on Haematology, Serum Biochemistry and Lipid Profile of ‎Stallions in Ilorin, Nigeria 年龄对尼日利亚伊洛林公马血液学、血清生化和血脂的影响
Pub Date : 2022-06-30 DOI: 10.54058/saheljvs.v19i2.302
Basiru, Sanusi A. O., F. Olaifa, Short Communication
This study determined the influence of age on haematological and serum biochemical parameters and lipid profiles of stallions in Ilorin, Nigeria. Blood samples of 50 (3-16 years of age) apparently healthy stallions were collected from different stables within Ilorin metropolis, and haematological and serum biochemical parameters were determined according to standard procedures. The stallions were grouped into three: <5 years (n=12), 6-11 years (n=33) and >11 years (n=5). The values obtained were subjected to a one-way ANOVA (p<0.05) using Tukey’s multiple comparison test as post test to determine the significant differences in the haematological and serum biochemical parameters in relation to age differences. Stallions with less than 5 years of age showed significant (p< 0.001) increase in PCV, red blood cell and haemoglobin counts when compared with stallions aged 6-11 years and above 11 years. Serum albumin and triglycerides for the <5 years of age stallion significantly reduced and increased respectively when compared to the stallions aged 6-11 years and above 11 years. There was no significant difference in cholesterol, white blood cell, neutrophils and lymphocytes counts. Age influences haematological and serum biochemical parameters in stallions. This is more noticeable in stallions aged five years and below. The findings from this study will guide clinicians in interpretations of haematological and serum biochemical values in different age group of stallions.
本研究确定了年龄对尼日利亚伊洛林种马血液学和血清生化参数以及脂质谱的影响。从伊洛林市区不同马厩采集50匹(3 ~ 16岁)明显健康的种马血液样本,按标准程序测定血清学和血清生化指标。这些种马被分为3组:11岁(n=5)。采用Tukey多重比较检验作为后验检验,对所得值进行单因素方差分析(p<0.05),以确定血液学和血清生化参数与年龄差异相关的显著性差异。与6-11岁及11岁以上的种马相比,5岁以下种马的PCV、红细胞和血红蛋白计数显著增加(p< 0.001)。与6 ~ 11岁和11岁以上的种马相比,<5岁的种马血清白蛋白和甘油三酯分别显著降低和升高。两组的胆固醇、白细胞、中性粒细胞和淋巴细胞计数无显著差异。年龄影响种马血液学和血清生化参数。这在5岁及以下的种马中更为明显。本研究结果将指导临床医生解释不同年龄组种马的血液学和血清生化值。
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引用次数: 0
In vitro Trypanocidal and Therapeutic activity of Aqueous leaves extract of Allium cepa linn in Experimentally infected Wistar rats 葱叶水提物对实验性感染Wistar大鼠的体外杀锥虫作用及治疗作用
Pub Date : 2022-06-30 DOI: 10.54058/saheljvs.v19i2.295
A. A. Biu, M. S. Auwal, U. A. Maina, S. Salihu, I. B. Kashimbe, L. Buratai, F. A. Lawan, P. A. Ruth
The aqueous leaf extract of Allium cepaL. was evaluated for its phytochemistry, acute toxicity, efficacy and in vitro trypanocidal activity using standard procedures. A total of 12 wistar rats were grouped into 4 groups of 3 rats each and were intraperitioneally administered with graded doses of 100, 200, 400 and 800 mg/kg bw of the extract. All groups of wistar rats were observed for 24 hours for sign of toxicity and mortality. Qualitative phytochemical analysis of the aqueous leaf extract revealed the presence of alkaloids,flavonoids, anthraquinones, steroids, saponins, reducing sugars, monosacharides, glycosides, tannins and ketones, while phlabotannins was absent. The median lethal dose (LD50) of the plant extract was determined as 600 mg/kg. Clinical signs exhibited in the treated wistar rats were dose dependent. The in vitro trypanocidal efficacy of the extract of A. cepa on Trypanosoma brucei showed lethality at 800 mg/ml.
葱叶的水提取物。采用标准程序对其植物化学、急性毒性、药效和体外锥虫活性进行评价。将12只wistar大鼠分为4组,每组3只,分别按100、200、400、800 mg/kg bw的剂量给药。各组wistar大鼠观察24小时毒性和死亡迹象。定性植物化学分析表明,水提物中含有生物碱、黄酮类、蒽醌类、类固醇、皂苷、还原糖、单糖、糖苷、单宁和酮类,而不含苯丹宁。测定该植物提取物的致死中位剂量(LD50)为600 mg/kg。治疗后wistar大鼠的临床症状呈剂量依赖性。cepa提取物对布鲁氏锥虫的体外杀虫效果在800 mg/ml时显示出致死性。
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引用次数: 0
Comparative Evaluation of Thiopental Sodium and Ketamine Hydrochloride ‎in the Maintenance of General Anaesthesia during Exploratory Laparotomy ‎in Nigerian Indigenous Dogs 硫喷妥钠与盐酸氯胺酮在尼日利亚土着犬剖腹探查术中维持全身麻醉的比较评价
Pub Date : 2022-06-30 DOI: 10.54058/saheljvs.v19i2.305
M. A. Sani, D. Sani, F. Khan, E. G. Emmanuel, S. Muhammad, A. A. Bada
This study was conducted to evaluate the effects of thiopental sodium and ketamine hydrochloride on rectal temperature, pulse and respiratory rates, anaesthetic duration/recovery time indogs during the maintenance of general anaesthesia post-induction with thiopental sodium. Ten apparently healthy male and female dogs, aged 12-30 months, weighing 7-14 kg were divided into two groups of 5dogs each. Atropine (0.02 mg/kg) and chlorpromazine (4 mg/kg) were used for premedication. Thiopental sodium (20 mg/kg) was used for induction and maintenance in group A while dogs in group B were given thiopental sodium (20 mg/kg) for induction and ketamine hydrochloride (20 mg/kg) for maintenance. Duration of anaesthesia to recovery time, and vital parameters were monitored and recorded accordingly. Results revealed group A had significantly (p<0.001) prolonged duration of anaesthesia to recovery time (400 minutes) when compared to group B (200 minutes). Significant decrease was however observed in rectal temperature of group A at 50 minutes (p< 0.05), 60-90 minutes (p<0.01) and100-110 minutes (p<0.001) when compared to group B. Similarly, the respiratory rates of dogs in group A showed significant decrease at 50 minutes (p< 0.01), 60-110 minutes (p< 0.001) when compared to group B. There was no significant (p> 0.05) effect in the pulse rates of either group. It was concluded that a relative shorter recovery time, stability in rectal temperature, pulse and respiratory rates in the group maintained with ketamine hydrochloride were observed when compared to the group induced and maintained with thiopental sodium.
本研究旨在评价硫喷妥钠和盐酸氯胺酮对犬在硫喷妥钠诱导后维持全身麻醉期间直肠温度、脉搏和呼吸频率、麻醉时间/恢复时间的影响。10只12-30个月大、体重7-14公斤、表面健康的公、母犬分为两组,每组5只。前用药为阿托品(0.02 mg/kg)和氯丙嗪(4 mg/kg)。A组采用硫喷妥钠(20 mg/kg)诱导维持,B组采用硫喷妥钠(20 mg/kg)诱导维持,盐酸氯胺酮(20 mg/kg)维持。监测麻醉时间至恢复时间及生命参数。结果显示,A组对两组患者脉搏率均有显著影响(p < 0.05)。结论:与硫喷妥钠诱导和维持组相比,盐酸氯胺酮维持组恢复时间较短,直肠温度、脉搏和呼吸频率稳定。
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引用次数: 1
Gross and Histological Studies of the Nasal Turbinates in Yankasa Sheep ‎‎(Ovis aries) in Maiduguri, Nigeria 尼日利亚迈杜古里Yankasa羊鼻鼻甲的大体和组织学研究
Pub Date : 2022-06-30 DOI: 10.54058/saheljvs.v19i2.270
I. Girgiri, M. K. Malah, M. H. Jiji, Y. Gazali
The present study was aimed at describing the anatomy of the nasal turbinates in adult Yankasa sheep using sectional anatomical planes and light microscopy. A total of 5 heads were used. The gross observations of the nasal turbinates were presented in midsagittal and serialized transverse sections. The nasal cavity comprised of dorsal, middle and ventral nasal turbinates. These turbinates were delineated by dorsal, middle, common and ventral nasal meatuses and presented distinct morphological details at different levels of the sectional planes. Microscopically, the mucosal lining of the nasal turbinates was divided into stratified squamous keratinized epithelium in the vestibular portion, pseudostratified columnar epithelium in the respiratory portion and olfactory pseudostratified columnar epithelium in the olfactory portion of the nasal cavity. The propria submucosa consisted of loose irregular connective tissue, having connective tissue cells, fibers, cavernous veins and cartilages. whereas the deeper part presented mixed glandular tissue of simple acinar and coiled tubular glands. The study provided basic information on gross and microscopic anatomy of the nasal turbinates in Yankasa sheep, which could serve as reference for clinical interpretation of diagnostic images of the head region of the Yankasa sheep, and for comparative anatomical studies.
本研究旨在利用断层解剖平面和光学显微镜描述成年羊鼻鼻甲的解剖结构。共使用5个正面。鼻甲骨的大体观察呈现在中矢状面和连续横切面上。鼻腔由背鼻甲、中鼻甲和腹鼻甲组成。鼻甲由鼻背、鼻中、鼻共、鼻腹组成,在不同的切面上呈现出不同的形态细节。镜下鼻甲粘膜内壁分为前庭部分层状鳞状角质化上皮、呼吸部分假层状柱状上皮和鼻腔嗅觉部分假层状柱状上皮。固有层粘膜下层由松散的不规则结缔组织组成,有结缔组织细胞、纤维、海绵状静脉和软骨。深层为单纯腺泡和卷曲管状腺的混合腺组织。本研究为雁卡萨羊鼻甲骨的大体和显微解剖提供了基础资料,可为雁卡萨羊头部区域诊断影像的临床解释和比较解剖学研究提供参考。
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引用次数: 0
Retrospective Study of Cattle Fetal Wastage at Hadeja Abattoir, Jigawa ‎State: Economic Implication and Seasonal Variation 吉加瓦州Hadeja屠宰场牛胎浪费的回顾性研究:经济意义和季节变化
Pub Date : 2022-03-31 DOI: 10.54058/saheljvs.v19i1.303
A. Zubairu, U. Haruna, D. Iliyasu, A. R. Mustapha, F. Lawan, M. Mustapha, L. Adamu, S. Muhammad
Fetal wastage usually occurs due to indiscriminate slaughter of pregnant cows without proper ante mortem assessment of the pregnancy status prior to slaughter in most of the abattoir in Jigawa state. Therefore, the current retrospective study was aimed to determine the extent of cattle fetal wastage and its economic implications for a period of 6 years (2016-2021) in Hadeja abattoir Jigawa state. The total number of cows slaughtered in Hadeja abattoir and the fetal wastage were evaluated. Variation in the number of fetal wastages during dry season and rainy season were determined. Economic implications of fetal wastage were evaluated. The results revealed the number of fetuses wasted were 3,763 within a six-year period. The highest number of fetal wastages 2777 (73.8 %/) were recorded in the dry season with (P < 0.05), compared to the number of fetal wastages 986 (26.2 %) recorded in the rainy season. The financial losses due to fetal wastages was estimated at ₦2,162,160,000 with an annual loss of ₦ 360,360,000. The yearly average fetal wastage across the study period was 627 (16.7 %). Large number of fetal wastages were recorded during the dry season with significant economic losses. Hence, effective ante mortem inspection and pregnancy diagnosis in cows is necessary in order to avert this problem at the abattoir. Similarly, reinforcement of legislation law against indiscriminate slaughter of pregnant cows should be enforced at the abattoir.
在吉加瓦州的大多数屠宰场,由于在屠宰前没有对怀孕状况进行适当的死前评估而随意屠宰怀孕奶牛,通常会发生胎儿流失。因此,目前的回顾性研究旨在确定吉加瓦州Hadeja屠宰场6年(2016-2021年)牛胎浪费的程度及其经济影响。对Hadeja屠宰场屠宰的奶牛总数和胎损进行了评价。测定了枯水期和雨季胎损数的变化。对胎儿浪费的经济意义进行了评估。结果显示,在6年的时间里,被浪费的胎儿数量为3763个。枯水期胎儿丢失2777例(73.8% /例),雨季胎儿丢失986例(26.2% /例),枯水期胎儿丢失最多(P < 0.05);胎儿浪费造成的经济损失估计为2,162,16万奈拉,每年损失为360,36万奈拉。在整个研究期间,年平均胎损为627例(16.7%)。旱季胎儿大量流失,造成重大经济损失。因此,为了在屠宰场避免这一问题,对奶牛进行有效的宰前检查和妊娠诊断是必要的。同样,应加强立法,在屠宰场执行禁止滥杀怀孕奶牛的法律。
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引用次数: 1
Hypoglycemic Response of Alloxan-Induced Diabetic Rats Fed with Sweet ‎Potato (Ipomoea Batatas) and Irish (Solanum Tuberosum) Potato Flour ‎Supplemented with Vitamins 甘薯(Ipomoea Batatas)和爱尔兰(Solanum Tuberosum)马铃薯粉(补充维生素)对四氧胺诱导的糖尿病大鼠的降糖反应
Pub Date : 2022-03-31 DOI: 10.54058/saheljvs.v19i1.284
B. B. Petrol, A. Abubakar, F. Sanda, M. Babakura, C. Goñi, M. Sherrif
The hypoglycemic effect of feeding normal and alloxan-induced diabetic rats with Sweet Potato (Ipomoea batatas)and Irish Potato (Solanum tuberosum)flour fortified with vitamin for 21 days was studied. Forty-five white albino rats were divided into groups of five rats each. The groups are Normal control, Normal control + diet, positive control, negative control, test control 1 and test control 2. Fasting blood glucose and 2 hours post-prandial were measured at 7 days, 14 days and 21 days. It was found that sweet potato and irish potato flour diet significantly (P<0.05) reduced the fasting blood glucose and 2 hours post-prandial blood glucose compared with those of diabetic and normal rats fed with control diet.  Maximum reduction in fasting and 2 hours post-prandial blood glucose was observed at day 21 with 119.6 ± 5.59 mmol/L in the sweet potato fed group compared to the diabetic control (223.6 ± 9.31mmol/L and normal control (106.6 ± 7.83) mmol/L. While the Irish potato group had (133 ± 2.45) mmol/L, compared to the diabetic control (233.6 ±9.31) mmol/L and normal control (106.6 ±7.83) mmol/L.  However Sweet potato showed a better hypoglycemic effect than irish potato. This shows that the hypoglycemic effect of sweet and irish potato is time dependent. 
研究了添加维生素的甘薯(Ipomoea batatas)和爱尔兰马铃薯(Solanum tuberosum)粉分别饲喂正常和四氧氧酮诱导的糖尿病大鼠21 d的降糖效果。45只白化大鼠被分成每组5只大鼠。各组分为正常对照组、正常对照组+饮食组、阳性对照组、阴性对照组、试验对照组1和试验对照组2。分别于第7天、第14天和第21天测定空腹血糖和餐后2小时血糖。结果发现,与对照组和糖尿病大鼠相比,红薯和爱尔兰土豆粉饲粮显著降低了糖尿病大鼠的空腹血糖和餐后2 h血糖(P<0.05)。与糖尿病对照组(223.6±9.31mmol/L)和正常对照组(106.6±7.83)mmol/L相比,红薯喂养组在第21天空腹和餐后2 h血糖下降幅度最大,为119.6±5.59 mmol/L。爱尔兰马铃薯组为(133±2.45)mmol/L,糖尿病对照组为(233.6±9.31)mmol/L,正常对照组为(106.6±7.83)mmol/L。甘薯的降糖效果优于爱尔兰马铃薯。这说明甘薯和爱尔兰薯的降糖作用是有时间依赖性的。
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引用次数: 0
Gross Morphological and Histological Features of Larynx in Yankasa Sheep ‎‎(Ovis aries)‎ Yankasa羊(Ovis aries)喉咙的大体形态和组织学特征
Pub Date : 2022-03-31 DOI: 10.54058/saheljvs.v19i1.294
I. Girgiri, M. K. Malah, A. Abdulrahman
The present study, conducted on the larynx of five adult Yankasa sheep of local breed, aimed at exploring its basic anatomy and histological features. The larynx consists of four cartilages; the unpaired epiglottis rostroventral, unpaired thyroid ventrally, dorsal paired arytenoid, and caudal unpaired cricoid. These cartilages presented distinct morphological features and were connected to each other by ligaments. The intrinsic laryngeal muscles include; the dorsal cricoarytenoid and transverse arytenoid muscles. The lateral cricoarytenoid was concealed by the thyroid laminae. The ventrally located thyroarytenoid and cricothyroid muscles. The laryngeal cavity comprised of rostral vestibule, a narrow middle glottic cleft and a wide infraglottic cavity caudally. Histologically, the epiglottis was lined by stratified squamous keratinized epithelium, the thyroid cartilage was lined by pseudostratified columnar epithelium, whereas the arytenoid cartilage was lined by pseudostratified columnar ciliated epithelium with goblet cells. The loose irregular loose connective tissue of the propria submucosa comprised of connective tissue cells mainly fibroblast, and fine blood capillaries, whereas the deeper part presented glandular tissues, ducts, fat cells and cartilages. It is envisaged that the study has provided basic information on gross and histological feature of the larynx in the Yankasa sheep.
本研究以5只当地品种的成年羊羊喉部为研究对象,探讨喉部的基本解剖和组织学特征。喉由四个软骨组成;未配对的会厌位于前腹侧,未配对的甲状腺位于腹侧,背侧为成对的杓状,尾侧为成对的环状。这些软骨具有明显的形态特征,并通过韧带相互连接。喉内肌包括;环杓背肌和杓横肌。侧环杓骨被甲状腺层所掩盖。位于腹侧的甲状关节和环甲肌。喉腔由吻侧前庭、狭窄的声门中裂和尾部宽的声门下腔组成。组织学上,会厌为层状鳞状角质化上皮,甲状软骨为假层状柱状上皮,而杓状软骨为带杯状细胞的假层状柱状纤毛上皮。固有层粘膜下层疏松不规则疏松结缔组织主要由成纤维细胞和细毛细血管组成,深层为腺组织、导管、脂肪细胞和软骨。预计该研究为羊喉的大体和组织学特征提供了基本信息。
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引用次数: 1
Environmental Assessment of Petrophilic Bacteria Associated with ‎Bioremediation and Biodegradation of Engine Oil Contaminated Soil in ‎Maiduguri 迈杜古里市发动机机油污染土壤生物修复与降解相关亲油细菌环境评价
Pub Date : 2021-12-31 DOI: 10.54058/saheljvs.v18i4.264
I. Hambali, I. Allamin, A. Oba, I. Salihu, F. U. Yarima, A. M. Hassan, F. A. Ali, N. Adamu, A. O. Tijjani, F. Jesse
Soil contamination by petroleum hydrocarbons is a major problem resulting from activities related to petroleum industry, automobile service stations and accidental release of oil in the soil. Bioremediation/biodegradation can be considered as an effective aid to clean up oil spills, aside from conventional methods that are uneconomical and produce residues that harm the surrounding biota. There are an estimated over 250 mechanic shops with more than 5000 technicians in Maiduguri. Mechanics working therein frequently spill oil which is a potent source of immunotoxicants and carcinogenic to humans and animals. The aim of this study was to screen for petrophilic bacterial isolates from engine oil contaminated soil, that can utilize crude oil as sole source of carbon for growth in Maiduguri Metropolis. Ten isolates from engine oil contaminated soils in Bola and Tashan Kano areas of Borno State were obtained. The bacteria were individually cultured in Bushnell Haas Mineral Salt Medium devoid of carbon source except for crude oil for 21 days at 370C. Bacterial growth from visible increased turbidity was enumerated by CFU/g on nutrient agar.  Pseudomonas sp. isolate exhibited relatively higher ability to grow on crude oil with TNTC followed by Bacillus sp., 6.7×104, Arthrobacter sp.,6.5×104, Flavobacterium sp.,6.2×104, Pseudomonas sp., 6×104, Nocardia sp.,5.9×104, Acinetobacter sp., 5×104, Bacillus sp., 5×104. Proteus sp., 4.7×104 and    Flavobacterium sp. 4×104.  It is therefore concluded that all the bacterial genera present in this study are effective hydrocarbon oil degraders.
石油烃对土壤的污染是石油工业、汽车加油站和石油在土壤中意外释放所造成的主要问题。生物修复/生物降解可以被认为是清理石油泄漏的有效辅助手段,除了传统的方法是不经济的和产生的残留物,损害周围的生物群。迈杜古里估计有250多家机修厂和5000多名技术人员。在那里工作的机械师经常泄漏石油,这是免疫毒物和对人类和动物致癌的有力来源。本研究的目的是从迈杜古里大都市区受机油污染的土壤中筛选可以原油为唯一碳源生长的亲石油细菌分离株。从博尔诺州博拉和塔山卡诺地区的发动机机油污染土壤中分离得到10株分离菌。在除原油外无碳源的Bushnell Haas无矿盐培养基中单独培养21 d,温度为370℃。在营养琼脂上以CFU/g计算可见浊度增加的细菌生长。假单胞菌在原油上的生长能力相对较高,其次是芽孢杆菌,6.7×104,节杆菌,6.5×104,黄杆菌,6.2×104,假单胞菌,6×104,诺卡菌,5.9×104,不动杆菌,5×104,芽孢杆菌,5×104。变形杆菌,4.7×104和黄杆菌4×104。因此,本研究中所有的细菌属都是有效的烃类油降解剂。
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引用次数: 0
期刊
Sahel Journal of Veterinary Sciences
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