Pub Date : 2023-06-30DOI: 10.15587/2519-8025.2023.285116
Denys Demianenko
The aim: study the bacterial risks and to determine the control critical points at the industrial production of chicken edible egg.
Materials and methods. The first stage of our research was to study the degree of risk of contamination by opportunistic and pathogenic microflora at all stages of production of chicken edible eggs according to ISO 22000:2007. In order to achieve the goal, bacteriological research was conducted on pathological material from day-old chickens and adult birds, the content of marketable eggs from birds of different age groups; as well as washed from the equipment of poultry farms, the repair young poultry department, the industrial herd department, egg sorting and certification workshops, from the surface of the egg, from the working surfaces of special vehicles. Bacterial contamination of air, droppings, bedding, complete feed was studied. Bacteriological studies were carried out according to generally accepted schemes, using accumulative, selective and differential diagnostic media (heptadecyl sulfate agar, endo, xylose-lysine agar, differentiated agar with diamond green, Muller-Hinton). The sampling was carried out using universal sterile applicators "Voles".
The next stage was to analyze the results of bacteriological studies and determine the basic list of criteria for creating critical control points (CCPs) for the further development of a risk management scheme for the bacterial biosafety of edible eggs according to HACCP principles.
Results. Isolation of S. aureus from the heart and lungs, E. coli, P. aeruginosa, Enterobacter spp, Enterococcus spp - from the intestines of day-old chickens and litter - indicate violations of veterinary and sanitary standards in the hatchery and the low quality of disinfection before the placement of day-old young birds, as well as non-observance of veterinary and sanitary norms for the transportation regime of day-old young birds. Isolation of E. coli, Enterobacter spp, Klebsiella spp.Str. zymogenes from compound feed, and E. coli, Enterobacter spp, Shigella spp. in washings from the equipment of the feed mill is evidence of insufficient veterinary and sanitary control of incoming raw materials, compound feed, as well as poorly carried out disinfection of equipment and specialized vehicles.When examining objects from the production line, E. coli, Enterobacter spp., P. aeruginosa, and S. epiderrmidis were most often isolated. From the pathological material during the bacteriological examination, microflora of the genus E. coli, Streptococcus spp., S. aureus (most often pathogenic serotypes) prevailed. As a result of the analysis of the conducted studies, we determined a basic list of criteria for creating a CCP and developing a risk management scheme for the bacterial biosafety of edible eggs according to the principles of HACCP.
Conclusions. As a result of the bacteriological monitoring of objects of the technological cycle of chicken edible egg production,
{"title":"Bacterial risks and determination of critical control points at the industrial production of chicken edible egg","authors":"Denys Demianenko","doi":"10.15587/2519-8025.2023.285116","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.285116","url":null,"abstract":"The aim: study the bacterial risks and to determine the control critical points at the industrial production of chicken edible egg.
 Materials and methods. The first stage of our research was to study the degree of risk of contamination by opportunistic and pathogenic microflora at all stages of production of chicken edible eggs according to ISO 22000:2007. In order to achieve the goal, bacteriological research was conducted on pathological material from day-old chickens and adult birds, the content of marketable eggs from birds of different age groups; as well as washed from the equipment of poultry farms, the repair young poultry department, the industrial herd department, egg sorting and certification workshops, from the surface of the egg, from the working surfaces of special vehicles. Bacterial contamination of air, droppings, bedding, complete feed was studied. Bacteriological studies were carried out according to generally accepted schemes, using accumulative, selective and differential diagnostic media (heptadecyl sulfate agar, endo, xylose-lysine agar, differentiated agar with diamond green, Muller-Hinton). The sampling was carried out using universal sterile applicators \"Voles\".
 The next stage was to analyze the results of bacteriological studies and determine the basic list of criteria for creating critical control points (CCPs) for the further development of a risk management scheme for the bacterial biosafety of edible eggs according to HACCP principles.
 Results. Isolation of S. aureus from the heart and lungs, E. coli, P. aeruginosa, Enterobacter spp, Enterococcus spp - from the intestines of day-old chickens and litter - indicate violations of veterinary and sanitary standards in the hatchery and the low quality of disinfection before the placement of day-old young birds, as well as non-observance of veterinary and sanitary norms for the transportation regime of day-old young birds. Isolation of E. coli, Enterobacter spp, Klebsiella spp.Str. zymogenes from compound feed, and E. coli, Enterobacter spp, Shigella spp. in washings from the equipment of the feed mill is evidence of insufficient veterinary and sanitary control of incoming raw materials, compound feed, as well as poorly carried out disinfection of equipment and specialized vehicles.When examining objects from the production line, E. coli, Enterobacter spp., P. aeruginosa, and S. epiderrmidis were most often isolated. From the pathological material during the bacteriological examination, microflora of the genus E. coli, Streptococcus spp., S. aureus (most often pathogenic serotypes) prevailed. As a result of the analysis of the conducted studies, we determined a basic list of criteria for creating a CCP and developing a risk management scheme for the bacterial biosafety of edible eggs according to the principles of HACCP.
 Conclusions. As a result of the bacteriological monitoring of objects of the technological cycle of chicken edible egg production,","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136365310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.15587/2519-8025.2023.276319
Hanna Stepanenko, O. Siehodin
The aim: Our particular interest in this study is not only the ability to extrapolate the experience of orthodontics of humane medicine for effective orthodontic correction in representatives of the animal world, but also the possibility of using teleroentgenometry and craniometry to study the skull of rodents and hare-like animals for the early preclinical diagnosis of dental disease. Materials and methods. The data of teleroentgenography (TRG), cranio- and gnatometry, biochemistry of connective tissue (GAG, GP, HST), fluoroscopy, densitometric parameters for early subclinical detection of dental disease in chinchillas (Chinchilla lanigera (n=20)), guinea pigs (Cavia porcellus (n=48)) and rabbits (Oryctolagus cuniculus(n=52)) are presented. All stages of the effective correction of mesial occlusion of incisors in rabbits (N=5) and dystropia of premolars in guinea pigs (N=5) are described. The camputation of efforts and points of their application that are necessary to move the tooth of the ellodont type is carried out. There are given the sequential stages of creating a dental imprint or 3D models, as well as the manufacture of fixed orthodontic structures, including an elastophore, orthodontic buttons with an Enlight Ormco fixation for incisors; and individual extraoral devices with expanding screws for premolars are presented. Results. Namely, among animals with dental disease, the following anatomical characteristics reliably took place. The basal angle of inclination of the base of the jaws to each other characterizing the vertical position of the jaws increased by 11 %; the body of the lower jaw shortened by 18 %; the height of the branches of the jaw increased by 17.5, and the mandibular angle, which is measured between the tangents to the lower edge of the lower jaw and the back surface of its branches, increased by 6 %. These data must be considered together with a reliable densitometric decrease in bone density and changes of biochemical components of the connective tissue in the blood serum. An analysis of bone strength of rabbits and guinea pigs is given in Tab. 2, which shows that the bone marrow of animals with dental history is statistically significantly different from the strength of animal bones without such among patients of rabbits and guinea pigs (p = 0.012 and p = 0.024, respectively). Thus, the method of program densitometry can be used to quantify the severity of metabolic disorders in the bone tissue to predict the further course of the reparative process, to appoint adequate pharmacological correction and to control the evaluation of therapeutic measures. Conclusions. The study of dental pathology of rodents and hare-like animals using densitometric, craniometric and biochemical methods allows detection of disorders in the early preclinical stage. And the extrapolation of the experience of humane orthodontics solves the issue of correcting the occlusion of these types of animals to restore the possibility of self-feed
{"title":"Orthodontic correction in rodents and hare-like animals: principles and methods of treatment","authors":"Hanna Stepanenko, O. Siehodin","doi":"10.15587/2519-8025.2023.276319","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.276319","url":null,"abstract":"The aim: Our particular interest in this study is not only the ability to extrapolate the experience of orthodontics of humane medicine for effective orthodontic correction in representatives of the animal world, but also the possibility of using teleroentgenometry and craniometry to study the skull of rodents and hare-like animals for the early preclinical diagnosis of dental disease. \u0000Materials and methods. The data of teleroentgenography (TRG), cranio- and gnatometry, biochemistry of connective tissue (GAG, GP, HST), fluoroscopy, densitometric parameters for early subclinical detection of dental disease in chinchillas (Chinchilla lanigera (n=20)), guinea pigs (Cavia porcellus (n=48)) and rabbits (Oryctolagus cuniculus(n=52)) are presented. All stages of the effective correction of mesial occlusion of incisors in rabbits (N=5) and dystropia of premolars in guinea pigs (N=5) are described. The camputation of efforts and points of their application that are necessary to move the tooth of the ellodont type is carried out. There are given the sequential stages of creating a dental imprint or 3D models, as well as the manufacture of fixed orthodontic structures, including an elastophore, orthodontic buttons with an Enlight Ormco fixation for incisors; and individual extraoral devices with expanding screws for premolars are presented. \u0000Results. Namely, among animals with dental disease, the following anatomical characteristics reliably took place. The basal angle of inclination of the base of the jaws to each other characterizing the vertical position of the jaws increased by 11 %; the body of the lower jaw shortened by 18 %; the height of the branches of the jaw increased by 17.5, and the mandibular angle, which is measured between the tangents to the lower edge of the lower jaw and the back surface of its branches, increased by 6 %. These data must be considered together with a reliable densitometric decrease in bone density and changes of biochemical components of the connective tissue in the blood serum. An analysis of bone strength of rabbits and guinea pigs is given in Tab. 2, which shows that the bone marrow of animals with dental history is statistically significantly different from the strength of animal bones without such among patients of rabbits and guinea pigs (p = 0.012 and p = 0.024, respectively). Thus, the method of program densitometry can be used to quantify the severity of metabolic disorders in the bone tissue to predict the further course of the reparative process, to appoint adequate pharmacological correction and to control the evaluation of therapeutic measures. \u0000Conclusions. The study of dental pathology of rodents and hare-like animals using densitometric, craniometric and biochemical methods allows detection of disorders in the early preclinical stage. And the extrapolation of the experience of humane orthodontics solves the issue of correcting the occlusion of these types of animals to restore the possibility of self-feed","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73919234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.15587/2519-8025.2023.275068
N. Seliukova, Yevgenia Korenieva, D. Morozenko, Yevheniia Vashchyk, Rymma Yeromenko, O. Matviichuk, Anatolii Matviichuk, Oleg Gladchenko
Exposure to phytoestrogens (PE) during prepuberty and puberty can modulate the functioning of the reproductive axis, causing irreversible damage to reproductive programming. The aim of the study was to investigate the state of the reproductive system of male offspring of adult rats that were exposed to phytoestrogens in the pubertal period of ontogenesis. Materials and methods. The work was performed on adult male and female Wistar rats and their male offspring. In the experiment, the biological effect of PE was studied when applying a dose of 20 mg/kg of body weight for 30 days to the father and/or offspring of puberty age starting from the 45th day of postnatal life. Upon reaching the age of six months, male offspring of all studied groups were examined for reproductive function. Results. The effect of estrogen-like substances on male reproductive function is manifested not only under the conditions of their intake in the critical periods of the embryonic and postnatal periods, but also, even when acting on the germ cells of parents. In male offspring, androgen secretion is disturbed, the hormonal status changes in the direction of hyperestrogenization, fertility decreases due to the reduced quality of germ cells against the background of a normal spermogram. Conclusion. The reproductive function of sexually mature male offspring of a phytoestrogenized father who received a mixture of phytoestrogens during puberty is characterized by differences in sexual behavior, a decrease in the reproductive potential of males, which occurs due to a decrease in the share of effective fertilization, which indicates negative changes in spermatozoa, the development of which took place in conditions of absolute and relative hyperestrogeny. This indicates that phytoestrogens, as an environmental factor, have adverse consequences not only for individuals who directly use them, but also for their male offspring
{"title":"The consequences of phytoestrogenization of the father and the effects of phytoestrogens during puberty for male offspring","authors":"N. Seliukova, Yevgenia Korenieva, D. Morozenko, Yevheniia Vashchyk, Rymma Yeromenko, O. Matviichuk, Anatolii Matviichuk, Oleg Gladchenko","doi":"10.15587/2519-8025.2023.275068","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.275068","url":null,"abstract":"Exposure to phytoestrogens (PE) during prepuberty and puberty can modulate the functioning of the reproductive axis, causing irreversible damage to reproductive programming. \u0000The aim of the study was to investigate the state of the reproductive system of male offspring of adult rats that were exposed to phytoestrogens in the pubertal period of ontogenesis. \u0000Materials and methods. The work was performed on adult male and female Wistar rats and their male offspring. In the experiment, the biological effect of PE was studied when applying a dose of 20 mg/kg of body weight for 30 days to the father and/or offspring of puberty age starting from the 45th day of postnatal life. Upon reaching the age of six months, male offspring of all studied groups were examined for reproductive function. \u0000Results. The effect of estrogen-like substances on male reproductive function is manifested not only under the conditions of their intake in the critical periods of the embryonic and postnatal periods, but also, even when acting on the germ cells of parents. In male offspring, androgen secretion is disturbed, the hormonal status changes in the direction of hyperestrogenization, fertility decreases due to the reduced quality of germ cells against the background of a normal spermogram. \u0000Conclusion. The reproductive function of sexually mature male offspring of a phytoestrogenized father who received a mixture of phytoestrogens during puberty is characterized by differences in sexual behavior, a decrease in the reproductive potential of males, which occurs due to a decrease in the share of effective fertilization, which indicates negative changes in spermatozoa, the development of which took place in conditions of absolute and relative hyperestrogeny. This indicates that phytoestrogens, as an environmental factor, have adverse consequences not only for individuals who directly use them, but also for their male offspring","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81861370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.15587/2519-8025.2023.276374
N. Sulashvili, N. Abuladze, Margarita Beglaryan, Jilda Cheishvili, Ada (Adel) Tadevosyan, Marika Sulashvil
Aim of the research was to study and analyze the features of opioids and narcotics using by drug addicted people and the role of replacement drugs in treatment and recovery of the patient. Materials and methods: We conducted a systematic review and meta-analysis of studies. Were used Systematic literature reviews and meta-analyses. The material of the article was data from the scientific literature, processed and analyzed by generalization and systematization. The scientific research ensues the fundamentals of assessment development of significant reviews. The ensuing databases were used: (for searching considerable literature to study and analyze the features of opioids and narcotics using by drug addiction people and the role of replacement drugs in treatment and recovery of the patient) Pub Med, Web of Science, Clinical key, Tomson Routers, Google Scholar, Cochrane Library, and Elsevier bases. Additionally studied national and internationals policy and guidelines and also grey literature. Results: Addiction is well known to be associated with its high level of physical and mental disorders. Most chronic chemical dependencies of drug addiction are also associated with its very high mortality rates, estimated to be several times those of the non-clinical population. Since addictive substances are known to disrupt cell growth and cell division, it can be assumed, that they particularly affect dividing cells, such as stem cell pools and progenitor cells. It is also known, that they either individually or in combination potentiate apoptosis, i.e., contribute to this effect. The medicine of aging in recent times has become an independent scientific discipline. The cellular aging hypothesis suggests the aging phenotype. The organism is associated with cellular correlates of age associated changes including cell loss, reduced cell velocity, renewal and more aging, negligible functional and non-replicating cells in tissues. So, the anti-growth effects of drug addiction can reasonably occur throughout the body. Expect signs of accelerated aging to be evident. One would expect such a putative progeroid effect to occur subject to increased morbidity and mortality rates, clinically observed almost identically in drug addicts as is the case in the geriatric population. In this connection there are various changes, consideration of all clinical aspects’ expression of this general toxicology hypothesis of opioids is needed. Conclusion: Pharmacological management of drug use should be only one component of treatment for drug needs, tailored to a comprehensive needs assessment of the child or young person, carried out in conjunction with appropriate psychological therapy and mental health interventions, and in the context of a clear and applied approach to the clinical management system. Caution is required when leaving these establishments due to the risk of overdose and in the transition to adult services. Physicians should carefully consider the degree of
研究的目的是研究和分析吸毒成瘾者使用阿片类药物和麻醉品的特点，以及替代药物在患者治疗和康复中的作用。材料和方法:我们对研究进行了系统回顾和荟萃分析。采用系统文献综述和荟萃分析。本文的资料来源于科学文献，经过归纳和系统化的处理和分析。科学研究遵循重大综述评价发展的基本原则。随后使用的数据库:(检索大量文献，研究和分析吸毒成瘾人群使用阿片类药物和麻醉品的特点以及替代药物在患者治疗和康复中的作用)Pub Med、Web of Science、Clinical key、Tomson Routers、Google Scholar、Cochrane Library和Elsevier数据库。此外，还研究了国家和国际政策和指导方针以及灰色文献。结果:众所周知，成瘾与高水平的身体和精神障碍有关。大多数药物成瘾的慢性化学依赖性也与极高的死亡率有关，据估计其死亡率是非临床人群的数倍。由于已知成瘾物质会破坏细胞生长和细胞分裂，可以假设，它们特别影响分裂细胞，如干细胞池和祖细胞。我们也知道，它们单独或联合促进细胞凋亡，即促成这种效应。衰老医学近年来已成为一门独立的科学学科。细胞老化假说提出了衰老表型。有机体与年龄相关的细胞变化相关，包括细胞损失，细胞速度降低，更新和更老化，组织中可忽略的功能和非复制细胞。因此，药物成瘾的抗生长作用可以合理地发生在全身。预计加速老化的迹象会很明显。人们可以预期，这种假定的类早衰效应会伴随着发病率和死亡率的增加而发生，在药物成瘾者和老年人群中临床观察到的情况几乎相同。在这方面有各种变化，需要考虑阿片类药物一般毒理学假设的所有临床方面的表达。结论:药物使用的药理学管理应只是药物需求治疗的一个组成部分，应根据儿童或青少年的综合需求评估进行调整，与适当的心理治疗和心理健康干预措施相结合，并在临床管理系统中采用明确和实用的方法。在离开这些机构时需要谨慎，因为有过量的风险，并在过渡到成人服务。医生应仔细考虑对任何物质的依赖程度，特别是当酒精和其他物质(如鸦片剂)同时使用时。充分实施治疗、康复和减少伤害服务将减少吸毒对个人、社区和整个社会的不良健康、社会和经济后果。参与咨询、治疗、康复和减少危害项目的吸毒者人数将会增加
{"title":"The manifestation of features of opioids and narcotics using by drug addicted people and the role of replacement pharmacotherapy in treatment and recovery of the patient","authors":"N. Sulashvili, N. Abuladze, Margarita Beglaryan, Jilda Cheishvili, Ada (Adel) Tadevosyan, Marika Sulashvil","doi":"10.15587/2519-8025.2023.276374","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.276374","url":null,"abstract":"Aim of the research was to study and analyze the features of opioids and narcotics using by drug addicted people and the role of replacement drugs in treatment and recovery of the patient. \u0000Materials and methods: We conducted a systematic review and meta-analysis of studies. Were used Systematic literature reviews and meta-analyses. The material of the article was data from the scientific literature, processed and analyzed by generalization and systematization. The scientific research ensues the fundamentals of assessment development of significant reviews. The ensuing databases were used: (for searching considerable literature to study and analyze the features of opioids and narcotics using by drug addiction people and the role of replacement drugs in treatment and recovery of the patient) Pub Med, Web of Science, Clinical key, Tomson Routers, Google Scholar, Cochrane Library, and Elsevier bases. Additionally studied national and internationals policy and guidelines and also grey literature. \u0000Results: Addiction is well known to be associated with its high level of physical and mental disorders. Most chronic chemical dependencies of drug addiction are also associated with its very high mortality rates, estimated to be several times those of the non-clinical population. Since addictive substances are known to disrupt cell growth and cell division, it can be assumed, that they particularly affect dividing cells, such as stem cell pools and progenitor cells. It is also known, that they either individually or in combination potentiate apoptosis, i.e., contribute to this effect. The medicine of aging in recent times has become an independent scientific discipline. The cellular aging hypothesis suggests the aging phenotype. The organism is associated with cellular correlates of age associated changes including cell loss, reduced cell velocity, renewal and more aging, negligible functional and non-replicating cells in tissues. So, the anti-growth effects of drug addiction can reasonably occur throughout the body. Expect signs of accelerated aging to be evident. One would expect such a putative progeroid effect to occur subject to increased morbidity and mortality rates, clinically observed almost identically in drug addicts as is the case in the geriatric population. In this connection there are various changes, consideration of all clinical aspects’ expression of this general toxicology hypothesis of opioids is needed. \u0000Conclusion: Pharmacological management of drug use should be only one component of treatment for drug needs, tailored to a comprehensive needs assessment of the child or young person, carried out in conjunction with appropriate psychological therapy and mental health interventions, and in the context of a clear and applied approach to the clinical management system. Caution is required when leaving these establishments due to the risk of overdose and in the transition to adult services. Physicians should carefully consider the degree of","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77681212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.15587/2519-8025.2023.277216
O. Filiptsova, O. Naboka, S. Bobro, O. Bashura, Yuliia Osypenko
The aim. The study represents a continuation of the previous one, and provide various aspects related to the use of perfumery, which can be characterized by certain associations with the education level of Ukrainian consumers. Factors that have direct or indirect biological and medical significance and, accordingly, may have a potential impact on the safety of the perfume products use by humans, were selected for the analysis. Materials and methods. The analysis included information about 124 individuals living in Ukraine. The respondents answered the questions of the questionnaire developed regarding some aspects of the perfumery use. The Pearson chi-squared (χ2) test was used to analyze the relationships between qualitative characteristics. Results and discussion. Most of the studied aspects related to the perfumes use demonstrate associations, caused by the dependence of the education level on age. The analysis revealed that individuals with any education most often (about half of the cases or more) choose a perfume based on its scent. The study showed that the majority of respondents believe that the price of a perfume depends on a combination of factors, namely the composition of the products, the brand and the cost of the packaging. More than 80 % of people, regardless of the education level, believe that the persistence of a perfume is definitely a sign of quality. The most common opinion among people of all education levels was that non-original perfumes can have a more negative effect on people's health than original products. The "lipstick effect" in relation to perfume products (demand for items of "affordable luxury" in times of economic crises and wars) was observed. Conclusions. The study supplemented the previously found associations of age and some biological and behavioral aspects of perfume use among the population of Ukraine with data on the presence or absence of such associations in relation to the education level
{"title":"Some biological, behavioral and social aspects of the perfumery use in the Ukrainian population sample (Part 2. Education level associations)","authors":"O. Filiptsova, O. Naboka, S. Bobro, O. Bashura, Yuliia Osypenko","doi":"10.15587/2519-8025.2023.277216","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.277216","url":null,"abstract":"The aim. The study represents a continuation of the previous one, and provide various aspects related to the use of perfumery, which can be characterized by certain associations with the education level of Ukrainian consumers. Factors that have direct or indirect biological and medical significance and, accordingly, may have a potential impact on the safety of the perfume products use by humans, were selected for the analysis. \u0000Materials and methods. The analysis included information about 124 individuals living in Ukraine. The respondents answered the questions of the questionnaire developed regarding some aspects of the perfumery use. The Pearson chi-squared (χ2) test was used to analyze the relationships between qualitative characteristics. \u0000Results and discussion. Most of the studied aspects related to the perfumes use demonstrate associations, caused by the dependence of the education level on age. The analysis revealed that individuals with any education most often (about half of the cases or more) choose a perfume based on its scent. The study showed that the majority of respondents believe that the price of a perfume depends on a combination of factors, namely the composition of the products, the brand and the cost of the packaging. More than 80 % of people, regardless of the education level, believe that the persistence of a perfume is definitely a sign of quality. The most common opinion among people of all education levels was that non-original perfumes can have a more negative effect on people's health than original products. The \"lipstick effect\" in relation to perfume products (demand for items of \"affordable luxury\" in times of economic crises and wars) was observed. \u0000Conclusions. The study supplemented the previously found associations of age and some biological and behavioral aspects of perfume use among the population of Ukraine with data on the presence or absence of such associations in relation to the education level","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"208 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74591279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.15587/2519-8025.2023.275588
O. Galatyuk, V. Yarovets, V. Babenko, V. Cherevatov, B. Gutyj, Andrii Grigorenko, M. Strilchuk, Ihor Stolyar
The uncontrolled spread of the subspecies A. m. carnica, A. m. ligustica, and A. m. caucasica has led to a reduction in the areas of pure "dark forest bees" populations belonging to the subspecies A. m. mellifera in their natural habitats within Ukraine. Due to the need to use dark forest bees in breeding, it became necessary to identify the locations of individual populations of bees belonging to the A. m. mellifera subspecies. The purpose of the work is to create an accessible and at the same time complete methodology for classifying bee wing phenotypes, which would make it possible to determine the probable breed of worker bees, the type and degree of hybridization of the main breed with impurities, and to identify "purebred" bee families by the wing phenotype suitable for further breeding. Material and methods of research: Using discriminant analysis of data, at the first stage of the study, 1500 wings of bee families were classified using 8 features: Ci, Dbi, Disc.sh, Pci, Ri, Сі.3, Сі.2, Сi.2.1, for which there was preliminary information about the possible belonging of the wing phenotype to the subspecies A. m. mellifera or its hybrids. At the second stage, additional 1212 wings of bee colonies were studied, about which there were doubts about their breed. Results of research and discussion: The wings are reliably divided into four clusters, indicating the presence of four sufficiently distinct groups among the studied wings in terms of phenotype. Conclusions and prospects for further research: A classification model has been created that allows for effective discrimination of the wings of working bees of bee colonies in Ukraine, the subspecies A. m. mellifera. Phenotypic values of indices of four Polissia micro-populations of bees, used as reference standards for possible hybridization detection, have been established, which can serve as standards in future research. Four colonies have been found, whose queens produce bees of the A. m. mellifera type of the Polissia population, and three colonies whose queens produce bees of the A. m. macedonica hybrid and can be used for further selection work
亚种a . m.b arnica、a . m.b liustica和a . m.b caucasica的不受控制的传播导致了属于a . m.b mellifera亚种的纯“黑森林蜜蜂”种群在乌克兰境内自然栖息地的面积减少。由于需要在繁殖中使用黑森林蜜蜂，因此有必要确定属于A. m. mellifera亚种的蜜蜂个体种群的位置。本工作的目的是建立一种易于理解且同时完整的蜜蜂翅膀表型分类方法，从而可以确定工蜂的可能品种，主要品种的杂交类型和程度，并通过翅膀表型确定适合进一步繁殖的“纯种”蜜蜂家族。研究材料和方法:采用数据的鉴别分析，在研究的第一阶段，利用Ci、Dbi、Disc.sh、Pci、Ri、Сі 8个特征对1500个蜜蜂科的翅膀进行分类。3,Сі。2， Сi.2.1，关于翅膀表型可能属于A. m. mellifera亚种或其杂交种的初步信息。第二阶段，对1212个蜂群的翅膀进行了额外的研究，对其品种存在疑问。研究和讨论的结果:翅膀被可靠地分为四个簇，表明在所研究的翅膀中存在四个充分不同的表型组。结论和进一步研究的前景:建立了一个分类模型，可以有效地区分乌克兰蜂群的工蜂翅膀，亚种A. m. mellifera。建立了4个蜜蜂Polissia微居群各指标的表型值，作为可能杂交检测的参考标准，可作为后续研究的标准。已经发现了4个蜂群，其蜂王生产的蜜蜂是波兰种群的A. m. mellifera类型，还有3个蜂群的蜂王生产的蜜蜂是A. m. macedonica杂交品种，可以用于进一步的选择工作
{"title":"II. Morphometry of wings of worker bees of the subspecies Apis mellifera mellifera L. (Polissya population of Zhytomyr region)","authors":"O. Galatyuk, V. Yarovets, V. Babenko, V. Cherevatov, B. Gutyj, Andrii Grigorenko, M. Strilchuk, Ihor Stolyar","doi":"10.15587/2519-8025.2023.275588","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.275588","url":null,"abstract":"The uncontrolled spread of the subspecies A. m. carnica, A. m. ligustica, and A. m. caucasica has led to a reduction in the areas of pure \"dark forest bees\" populations belonging to the subspecies A. m. mellifera in their natural habitats within Ukraine. Due to the need to use dark forest bees in breeding, it became necessary to identify the locations of individual populations of bees belonging to the A. m. mellifera subspecies. \u0000The purpose of the work is to create an accessible and at the same time complete methodology for classifying bee wing phenotypes, which would make it possible to determine the probable breed of worker bees, the type and degree of hybridization of the main breed with impurities, and to identify \"purebred\" bee families by the wing phenotype suitable for further breeding. \u0000Material and methods of research: Using discriminant analysis of data, at the first stage of the study, 1500 wings of bee families were classified using 8 features: Ci, Dbi, Disc.sh, Pci, Ri, Сі.3, Сі.2, Сi.2.1, for which there was preliminary information about the possible belonging of the wing phenotype to the subspecies A. m. mellifera or its hybrids. At the second stage, additional 1212 wings of bee colonies were studied, about which there were doubts about their breed. \u0000Results of research and discussion: The wings are reliably divided into four clusters, indicating the presence of four sufficiently distinct groups among the studied wings in terms of phenotype. \u0000Conclusions and prospects for further research: A classification model has been created that allows for effective discrimination of the wings of working bees of bee colonies in Ukraine, the subspecies A. m. mellifera. Phenotypic values of indices of four Polissia micro-populations of bees, used as reference standards for possible hybridization detection, have been established, which can serve as standards in future research. Four colonies have been found, whose queens produce bees of the A. m. mellifera type of the Polissia population, and three colonies whose queens produce bees of the A. m. macedonica hybrid and can be used for further selection work","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83739800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.15587/2519-8025.2023.275942
O. Naboka, A. Kotvitska, N. Filimonova, A. Glushchenko, O. Filiptsova, A. Volkova
Scientific data on the pharmacodynamics of dry extracts of Bupleurum aureum and Salsola collina L. based on the results of studying the antimicrobial effect and the similar effect of co-trimoxazole when they are used together have been supplemented. The investigated phytoextracts do not show antimicrobial properties, but they do not change the antimicrobial effect of co-trimoxazole when they are used in combination. The aim of the study was to experimentally investigate the antimicrobial effect of extracts of Bupleurum aureum and Salsola collina L. and establish the possible antagonistic effect of these extracts on the antimicrobial drug co-trimoxazole when used together. Materials and methods. The research was conducted in May 2016. Screening of the antimicrobial effect of extracts of Bupleurum aureum and Salsola collina L. and establishing of the possible antagonistic effect of these extracts on the antimicrobial drug co-trimoxazole when they are used together was carried out in the laboratory of the Department of Microbiology of the National Pharmaceutical University, which has a certificate of attestation 045/14 dated 28.10.2014. For determination of antimicrobial activity, the agar diffusion method ("well" method), which is based on the ability of medicinal substances to penetrate the agar layer, was used. A set of reference strains of microorganisms was used: S. aureus ATCC 6538, E. coli ATCC 8739, P. aeruginosa ATCC 9027, B. subtilis ATCC 6633, C. albicans ATCC 10231. Petri dishes were filled with two layers of solid nutrient medium. The lower layer - 10 ml of melted "cold" AGV agar (medium No. 3), the upper layer - nutrient medium for the corresponding test strain. After cooling the lower layer of agar, three thin-walled steel cylinders (inner diameter - 6.0±0.1 mm, height - 10.0±0.1 mm) were placed on it at an equal distance from each other and from the edge of the cup. The top layer was poured around the cylinders - 13.5 ml of agar, melted and cooled to 45-48°С, mixed with the seed dose of the test microorganism (1.5 ml of microbial suspension, the concentration corresponding to the type of microorganism). After cooling the upper layer of agar, the cylinders were removed with sterile tweezers and 0.25-0.3 ml of the studied drug was added to the resulting wells. The results were recorded after 24 h by measuring the zone of growth inhibition, including the diameter of the wells. Measurements were made with an accuracy of 1 mm, while focusing on the complete absence of visible growth. The obtained data were analyzed using the methods of variational statistics. The significance level is p<0.05. The studied plant extracts of Bupleurum aureum (aqueous and alcoholic) and Salsola collina L. (aqueous and alcoholic) were used in doses of 0.005 mg/ml and 0.01 mg/ml, which corresponded to doses of 5 mg/kg and 10 mg/kg. Experimental data were also processed by parametric (Newman-Keuls) and non-parametric (Mann-Whitney) methods of variational stat
{"title":"Investigation of the influence of dry extracts of bupleurum aureum and Salsola collina L. on the antimicrobial effect of co-trimoxazole","authors":"O. Naboka, A. Kotvitska, N. Filimonova, A. Glushchenko, O. Filiptsova, A. Volkova","doi":"10.15587/2519-8025.2023.275942","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.275942","url":null,"abstract":"Scientific data on the pharmacodynamics of dry extracts of Bupleurum aureum and Salsola collina L. based on the results of studying the antimicrobial effect and the similar effect of co-trimoxazole when they are used together have been supplemented. The investigated phytoextracts do not show antimicrobial properties, but they do not change the antimicrobial effect of co-trimoxazole when they are used in combination. \u0000The aim of the study was to experimentally investigate the antimicrobial effect of extracts of Bupleurum aureum and Salsola collina L. and establish the possible antagonistic effect of these extracts on the antimicrobial drug co-trimoxazole when used together. \u0000Materials and methods. The research was conducted in May 2016. Screening of the antimicrobial effect of extracts of Bupleurum aureum and Salsola collina L. and establishing of the possible antagonistic effect of these extracts on the antimicrobial drug co-trimoxazole when they are used together was carried out in the laboratory of the Department of Microbiology of the National Pharmaceutical University, which has a certificate of attestation 045/14 dated 28.10.2014. For determination of antimicrobial activity, the agar diffusion method (\"well\" method), which is based on the ability of medicinal substances to penetrate the agar layer, was used. A set of reference strains of microorganisms was used: S. aureus ATCC 6538, E. coli ATCC 8739, P. aeruginosa ATCC 9027, B. subtilis ATCC 6633, C. albicans ATCC 10231. Petri dishes were filled with two layers of solid nutrient medium. The lower layer - 10 ml of melted \"cold\" AGV agar (medium No. 3), the upper layer - nutrient medium for the corresponding test strain. After cooling the lower layer of agar, three thin-walled steel cylinders (inner diameter - 6.0±0.1 mm, height - 10.0±0.1 mm) were placed on it at an equal distance from each other and from the edge of the cup. The top layer was poured around the cylinders - 13.5 ml of agar, melted and cooled to 45-48°С, mixed with the seed dose of the test microorganism (1.5 ml of microbial suspension, the concentration corresponding to the type of microorganism). After cooling the upper layer of agar, the cylinders were removed with sterile tweezers and 0.25-0.3 ml of the studied drug was added to the resulting wells. The results were recorded after 24 h by measuring the zone of growth inhibition, including the diameter of the wells. Measurements were made with an accuracy of 1 mm, while focusing on the complete absence of visible growth. \u0000The obtained data were analyzed using the methods of variational statistics. The significance level is p<0.05. The studied plant extracts of Bupleurum aureum (aqueous and alcoholic) and Salsola collina L. (aqueous and alcoholic) were used in doses of 0.005 mg/ml and 0.01 mg/ml, which corresponded to doses of 5 mg/kg and 10 mg/kg. Experimental data were also processed by parametric (Newman-Keuls) and non-parametric (Mann-Whitney) methods of variational stat","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91316558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.15587/2519-8025.2022.266237
D. Morozenko, Yevheniia Vashchyk, A. Zakhariev, Dmytro Berezhnyi, N. Seliukova, K. Gliebova
The aim: to determine the main pathogenetic links of glomerulonephritis in dogs with the participation of connective tissue biopolymers. Materials and methods The research was conducted by analyzing the sources of scientific literature (PubMed, Elsevier, electronic resources of the V. I. Vernadskyi National Library) and the clinical experience of the authors, thanks to which a scheme of the pathogenesis of glomerulonephritis in dogs with the participation of connective tissue biopolymers was developed. Results. According to the results of our research, it was established, that chronic glomerulonephritis in dogs is accompanied by neutrophilia, lymphocytosis, as well as urinary and nephrotic syndromes, the progression of which causes a violation of the functional state of the kidneys and liver. Sick dogs develop a nephrotic symptom complex – persistent proteinuria, hypoalbuminemia, dysproteinemia, and hyperlipidemia. Clinically, edema was not observed, because it is known, that in dogs they are rarely detected with nephrotic syndrome. Inflammation in the kidneys is accompanied by an increase in the concentration of glycoproteins and sialic acids in the blood serum. After treatment, there was a decrease in the inflammatory process in the kidneys, which was manifested by a decrease in neutrophilia and lymphocytosis, as well as the content of glycoproteins and sialic acids in the blood serum. The content of total chondroitin sulfates and the fractional composition of glycosaminoglycans did not change, and the level of excretion of oxyproline and uronic acids decreased compared to the indicators before the start of treatment. This, in our opinion, is due to the slowing down of fibrotic processes in the kidneys. Thus, biochemical indicators of the state of connective tissue in the blood serum and urine of dogs with glomerulonephritis allowed us to evaluate the functional state of the kidneys for their inflammation (oxyproline and uronic acids), as well as to determine the violation of proteoglycan synthesis in nephrotic syndrome. Conclusions. For glomerulonephritis in dogs on the background of depression, decreased appetite, polydipsia, pain during palpation in the lumbar region, periodic vomiting, neutrophilic leukocytosis, lymphocytosis, nephrotic syndrome (hypoalbuminemia, increase in α2- and β-globulins, cholesterol, β-lipoproteins), growth activity of ALT, AST, and alkaline phosphatase, a decrease in Veltman's test, hyperazotemia, hyperphosphatemia, proteinuria, microhematuria, and cylindruria, there is an increase in the content of glycoproteins, sialic acids, and chondroitin sulfates, as well as heparan sulfate in the blood serum of dogs. The increase in the blood serum of patients with canine glomerulonephritis, as a marker of connective tissue – glycoproteins, sialic acids, chondroitinsulfates, and urinary excretion of oxyproline and uronic acids is due to inflammatory and fibrotic changes in the basal membranes of the kidney glomeruli
目的:确定结缔组织生物聚合物参与犬肾小球肾炎的主要致病环节。材料与方法通过对科学文献(PubMed, Elsevier, V. I. Vernadskyi国家图书馆电子资源)的来源和作者的临床经验进行分析，制定了结缔组织生物聚合物参与犬肾小球肾炎发病机制的方案。结果。根据我们的研究结果，确定犬慢性肾小球肾炎伴中性粒细胞增多、淋巴细胞增多以及泌尿和肾病综合征，其进展会导致肾脏和肝脏功能状态的破坏。患病的狗会出现复杂的肾病症状——持续性蛋白尿、低白蛋白血症、蛋白异常血症和高脂血症。在临床上，没有观察到水肿，因为众所周知，在狗中很少发现有肾病综合征。肾脏的炎症伴随着血清中糖蛋白和唾液酸浓度的增加。治疗后，肾脏炎症过程减轻，表现为嗜中性粒细胞和淋巴细胞增多，血清中糖蛋白和唾液酸含量降低。与治疗前相比，总硫酸软骨素含量和糖胺聚糖分数组成没有变化，羟脯氨酸和糖醛酸排泄水平下降。在我们看来，这是由于肾脏纤维化过程的减慢。因此，肾小球肾炎犬血清和尿液中结缔组织状态的生化指标使我们能够评估肾脏的炎症功能状态(氧脯氨酸和尿酸)，并确定肾病综合征中蛋白多糖合成的违反。结论。犬的肾小球肾炎，伴有抑郁、食欲减退、多饮、腰痛、周期性呕吐、嗜中性粒细胞增多、淋巴细胞增多、肾病综合征(低白蛋白血症、α2-和β-球蛋白、胆固醇、β-脂蛋白升高)、ALT、AST和碱性磷酸酶生长活性、Veltman试验降低、高氮血症、高磷血症、蛋白尿、微量血尿和柱状尿，狗的血清中糖蛋白、唾液酸、硫酸软骨素和硫酸肝素含量增加。犬肾小球肾炎患者血清中作为结缔组织糖蛋白、唾液酸、软骨素、尿中氧脯氨酸和尿醛酸排泄标志的升高是由于肾小球基底膜的炎症和纤维化改变
{"title":"The pathogenetic role of glycoproteins and proteoglycans in dog glomerulonephritis","authors":"D. Morozenko, Yevheniia Vashchyk, A. Zakhariev, Dmytro Berezhnyi, N. Seliukova, K. Gliebova","doi":"10.15587/2519-8025.2022.266237","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.266237","url":null,"abstract":"The aim: to determine the main pathogenetic links of glomerulonephritis in dogs with the participation of connective tissue biopolymers. \u0000Materials and methods The research was conducted by analyzing the sources of scientific literature (PubMed, Elsevier, electronic resources of the V. I. Vernadskyi National Library) and the clinical experience of the authors, thanks to which a scheme of the pathogenesis of glomerulonephritis in dogs with the participation of connective tissue biopolymers was developed. \u0000Results. According to the results of our research, it was established, that chronic glomerulonephritis in dogs is accompanied by neutrophilia, lymphocytosis, as well as urinary and nephrotic syndromes, the progression of which causes a violation of the functional state of the kidneys and liver. Sick dogs develop a nephrotic symptom complex – persistent proteinuria, hypoalbuminemia, dysproteinemia, and hyperlipidemia. Clinically, edema was not observed, because it is known, that in dogs they are rarely detected with nephrotic syndrome. Inflammation in the kidneys is accompanied by an increase in the concentration of glycoproteins and sialic acids in the blood serum. After treatment, there was a decrease in the inflammatory process in the kidneys, which was manifested by a decrease in neutrophilia and lymphocytosis, as well as the content of glycoproteins and sialic acids in the blood serum. The content of total chondroitin sulfates and the fractional composition of glycosaminoglycans did not change, and the level of excretion of oxyproline and uronic acids decreased compared to the indicators before the start of treatment. This, in our opinion, is due to the slowing down of fibrotic processes in the kidneys. Thus, biochemical indicators of the state of connective tissue in the blood serum and urine of dogs with glomerulonephritis allowed us to evaluate the functional state of the kidneys for their inflammation (oxyproline and uronic acids), as well as to determine the violation of proteoglycan synthesis in nephrotic syndrome. \u0000Conclusions. For glomerulonephritis in dogs on the background of depression, decreased appetite, polydipsia, pain during palpation in the lumbar region, periodic vomiting, neutrophilic leukocytosis, lymphocytosis, nephrotic syndrome (hypoalbuminemia, increase in α2- and β-globulins, cholesterol, β-lipoproteins), growth activity of ALT, AST, and alkaline phosphatase, a decrease in Veltman's test, hyperazotemia, hyperphosphatemia, proteinuria, microhematuria, and cylindruria, there is an increase in the content of glycoproteins, sialic acids, and chondroitin sulfates, as well as heparan sulfate in the blood serum of dogs. The increase in the blood serum of patients with canine glomerulonephritis, as a marker of connective tissue – glycoproteins, sialic acids, chondroitinsulfates, and urinary excretion of oxyproline and uronic acids is due to inflammatory and fibrotic changes in the basal membranes of the kidney glomeruli","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82300468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.15587/2519-8025.2022.266536
D. Morozenko, Yevheniia Vashchyk, A. Zakhariev, N. Seliukova, Dmytro Berezhnyi, K. Gliebova
The aim: to analyze clinical cases of diabetes mellitus in cats and establish the effectiveness of clinical and laboratory research and treatment of animals with the help of insulin therapy. Materials and methods. The material for the study were domestic cats admitted to the veterinary medicine clinic "Doctor Vet" (Lviv). Results. Clinical case 1. Cat Bonya, age 10 years, body weight 4 kg. symptoms: polyuria/polydipsia and weight loss. Examination: cachexia, weakness, pale mucous membranes, unsteady gait. Blood glucose - 20.4 mmol/l, urine analysis - pH=6.0, glucose ++++, no ketones detected. Diagnosis: diabetes. For glycemic control - caninsulin at 0.25 IU/kg every 12 hours. After the start of insulin therapy, the animal's condition improved, after 2 months - an attack of hypoglycemia, blood glucose 3.0 mmol/l. The introduction of insulin was stopped for 10 days, after 10 days the cat's appetite is normal, polyuria and polydipsia are not observed, body weight gain is 300 g, blood glucose without insulin therapy is 8.0 mmol/l. Clinical case 2. Cat Eva, age 12 years, body weight 6.7 kg, symptoms: polyuria/polydipsia during the last two weeks, increased appetite, diet - raw meat, meatballs, buckwheat porridge, boiled carrots, beets. Blood glucose 22.9 mmol/l, urine analysis – pH = 6.0, glucose ++++, ketones +. Diagnosis: diabetes. Treatment: Lantus 2 IU subcutaneously every 12 hours. After the start of insulin therapy, the condition improved, after 2 months, blood glucose was 9.00 - 13.0 mmol/l; 15.00 – 8.0 mmol/l, 21.00 – 12.0 mmol/l, glucose and ketones are absent in the urine. Conclusions. In the first clinical case, the cat there was a decrease in demand in insulin “Caninsulin” on the background of insulin therapy. In the second clinical case, insulin therapy with the help of "Lantus" insulin allows you to reach a level of glycemia at which diabetes stops progressing, the clinical condition of the animal normalizes, which indicates the effectiveness of the prescribed treatment. Thus, we can say that diabetes in small domestic animals is a rather severe endocrine pathology, which has typical clinical and laboratory symptoms and can be successfully treated with the help of a complex medical and diagnostic approach, based on insulin therapy
{"title":"Diabetes mellitus in domestic cats: clinical cases from veterinary practice","authors":"D. Morozenko, Yevheniia Vashchyk, A. Zakhariev, N. Seliukova, Dmytro Berezhnyi, K. Gliebova","doi":"10.15587/2519-8025.2022.266536","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.266536","url":null,"abstract":"The aim: to analyze clinical cases of diabetes mellitus in cats and establish the effectiveness of clinical and laboratory research and treatment of animals with the help of insulin therapy. \u0000Materials and methods. The material for the study were domestic cats admitted to the veterinary medicine clinic \"Doctor Vet\" (Lviv). \u0000Results. Clinical case 1. Cat Bonya, age 10 years, body weight 4 kg. symptoms: polyuria/polydipsia and weight loss. Examination: cachexia, weakness, pale mucous membranes, unsteady gait. Blood glucose - 20.4 mmol/l, urine analysis - pH=6.0, glucose ++++, no ketones detected. Diagnosis: diabetes. For glycemic control - caninsulin at 0.25 IU/kg every 12 hours. After the start of insulin therapy, the animal's condition improved, after 2 months - an attack of hypoglycemia, blood glucose 3.0 mmol/l. The introduction of insulin was stopped for 10 days, after 10 days the cat's appetite is normal, polyuria and polydipsia are not observed, body weight gain is 300 g, blood glucose without insulin therapy is 8.0 mmol/l. \u0000Clinical case 2. Cat Eva, age 12 years, body weight 6.7 kg, symptoms: polyuria/polydipsia during the last two weeks, increased appetite, diet - raw meat, meatballs, buckwheat porridge, boiled carrots, beets. Blood glucose 22.9 mmol/l, urine analysis – pH = 6.0, glucose ++++, ketones +. Diagnosis: diabetes. Treatment: Lantus 2 IU subcutaneously every 12 hours. After the start of insulin therapy, the condition improved, after 2 months, blood glucose was 9.00 - 13.0 mmol/l; 15.00 – 8.0 mmol/l, 21.00 – 12.0 mmol/l, glucose and ketones are absent in the urine. \u0000Conclusions. In the first clinical case, the cat there was a decrease in demand in insulin “Caninsulin” on the background of insulin therapy. In the second clinical case, insulin therapy with the help of \"Lantus\" insulin allows you to reach a level of glycemia at which diabetes stops progressing, the clinical condition of the animal normalizes, which indicates the effectiveness of the prescribed treatment. Thus, we can say that diabetes in small domestic animals is a rather severe endocrine pathology, which has typical clinical and laboratory symptoms and can be successfully treated with the help of a complex medical and diagnostic approach, based on insulin therapy","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78352343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.15587/2519-8025.2022.271039
O. Dolzhykova, R. Yeromenko
The aim. to study the therapeutic influence of new pessaries Melanizol based on metronidazole and tea tree oil on the background of model of “chemical” vaginitis in female rats. Materials and methods. The therapeutic effect of pessaries Melanizol was studied in female rats on the model of "chemical" vaginitis with using argentum nitrate, which provokes vaginitis not burdened by infection. Against the background of vaginitis, the general condition of animals, the dynamics of body weight, the morphological composition of peripheral blood and the erythrocyte sedimentation rate (ESR), pH, biochemical parameters (total protein, ALT and glucose), temperature in the vagina of rats, macroscopically assessed changes in the vaginal mucosa (VM) were studied. Results and discussion. Against the background of pathology in the animals, a shift in the studied indicators was observed. Pessaries Melanizol at the dose of 21 mg/kg make a therapeutic effect on the model of experimental "chemical" vaginitis, caused by argentum nitrate, as indicated by the restoration of the VM state, reduced the number of leukocytes, restored the percentage composition of the leukocyte formula, reduced the amount of total protein and the cytolytic enzyme ALT in blood serum and vaginal contents, which indicates the anti-inflammatory and cytoprotective effect of the studied agent. The recovery of vaginal temperature, pH and the amount of glucose in the vaginal contents was also observed, which indicates the normalization of homeostasis in the vagina of experimental animals. According to these effects, the studied pessaries Melanizol were superior to the reference drug "Gravagin" at the dose of 30 mg/kg and were not inferior to the reference drug Hippophaes oleum pessaries (HOP) at the dose of 17.64 mg/kg. Conclusions. The therapeutic effect of new pessaries Melanizol based on metronidazole and tea tree oil on the model of "chemical" vaginitis in female rats has been proven. The investigated pessaries Melanizol are a promising drug for the treatment of non-specific vaginitis and require further research in this direction
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