Pub Date : 2022-12-30DOI: 10.15587/2519-8025.2022.271049
O. Naboka, L. Vyshnevska, Inna Pasynchuk, O. Filiptsova, O. Tkachenko, Olha Vislous
Aim: the aim of the research is a pharmacological study of the original extracts of corn silk. Materials and methods. The object of the study were 4 original extracts of corn silk, obtained by extraction with water and ethanol of different concentrations, namely: aqueous extract of corn silk (ACSE), extract of corn silk, extracted with ethanol 30 % (ECSE 30 %) , extract of corn silk, extracted with ethanol 50 % (ЕCSЕ 50 %), extract of corn silk, extracted with ethanol 70 % (ЕCSЕ 70 %). A screening study of the antioxidant properties of corn silk extracts was carried out in vitro using models of spontaneous and ascorbate-induced lipid peroxidation (LPO) in rat liver homogenate. Results. As can be seen from the given data, ECSE 50 % at a dose of 20 mg/kg showed a pronounced protective effect on the hepatotoxicity of TСM. Its use led to a probable restoration of the bile-forming function of the liver against the background of tetrachloromethane hepatitis: in response to an increase in the cholesterol content (by 43 %, p<0.05), the content of bile acids increased 2 times (p<0.05), as a result of which cholate -cholesterol ratio approached the level of indicators of intact animals, the rate of bile secretion normalized. Based on the analysis of the results of the study, it can be concluded, that in terms of bile-forming and choleretic activity on the tetrachloromethane hepatitis model, ECSE 50 % was not inferior to silibor and superior to quercetin (р<0.05). Conclusions. Biologically active substances of corn silk affect not only the diffusion and filtration processes of the liver parenchyma, but also the biosynthesis and transport of its organic components, that is, they affect the bile-forming function
{"title":"Pharmacological study of original extracts of corn silk","authors":"O. Naboka, L. Vyshnevska, Inna Pasynchuk, O. Filiptsova, O. Tkachenko, Olha Vislous","doi":"10.15587/2519-8025.2022.271049","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.271049","url":null,"abstract":"Aim: the aim of the research is a pharmacological study of the original extracts of corn silk.\u0000Materials and methods. The object of the study were 4 original extracts of corn silk, obtained by extraction with water and ethanol of different concentrations, namely: aqueous extract of corn silk (ACSE), extract of corn silk, extracted with ethanol 30 % (ECSE 30 %) , extract of corn silk, extracted with ethanol 50 % (ЕCSЕ 50 %), extract of corn silk, extracted with ethanol 70 % (ЕCSЕ 70 %). A screening study of the antioxidant properties of corn silk extracts was carried out in vitro using models of spontaneous and ascorbate-induced lipid peroxidation (LPO) in rat liver homogenate.\u0000Results. As can be seen from the given data, ECSE 50 % at a dose of 20 mg/kg showed a pronounced protective effect on the hepatotoxicity of TСM. Its use led to a probable restoration of the bile-forming function of the liver against the background of tetrachloromethane hepatitis: in response to an increase in the cholesterol content (by 43 %, p<0.05), the content of bile acids increased 2 times (p<0.05), as a result of which cholate -cholesterol ratio approached the level of indicators of intact animals, the rate of bile secretion normalized. Based on the analysis of the results of the study, it can be concluded, that in terms of bile-forming and choleretic activity on the tetrachloromethane hepatitis model, ECSE 50 % was not inferior to silibor and superior to quercetin (р<0.05).\u0000Conclusions. Biologically active substances of corn silk affect not only the diffusion and filtration processes of the liver parenchyma, but also the biosynthesis and transport of its organic components, that is, they affect the bile-forming function","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75144518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.15587/2519-8025.2022.271043
O. Filiptsova, O. Bashura, O. Naboka, Oleksandra Kran, M. Almakaiev, H. Kukhtenko, T. Martyniuk, Yuliia Osypenko
The aim. The study encompassed some aspects related to perfumes use, which can potentially influence the individual perfume choice, in particular, among different age groups of the Ukrainian population. Factors related to perfumes choice, the state of awareness of respondents regarding quality, safety, and existing stereotypes regarding the characteristics of perfumes and potential harmful effects on the human body were investigated. Materials and methods. The analysis was performed on a sample of 124 residents of Ukraine. Respondents, who were randomly selected, were offered a questionnaire, developed by us, which included 15 items on the demographic characteristics of the subjects and questions related to the perfume use of and stereotypes about them. The Pearson chi-squared (χ2) test was used to analyze the relationships between qualitative characteristics. Results and discussion. About 98 % of surveyed persons in Ukraine use perfumery. Associations were found between age and the probability of purchase by the study participants of non-original perfume products and awareness of the safety of various perfume products. It has been established, that the determining factors when choosing a perfume is a certain scent, while the brand is given secondary importance. An increase in the effect of the brand in older age groups of the population has been demonstrated. Behavioral features of perfume use have been established: in younger women, it is associated with increased self-esteem and the influence of social networks, with age, the focus shifts to the desire for a positive effect on the mood from the use of a certain fragrance. However, in all age groups, the main purpose of perfume using was to improve the emotional state. Middle-aged women are more democratic in choosing perfumery with a specific gender orientation. Conclusions. For the first time in Ukraine, a pilot study was conducted to find possible associations between some biological, behavioral and demographic characteristics and the perfume use. Such factors as age and education level are promising for further analysis of the selected issues
{"title":"Some biological, behavioral and social aspects of the perfumery use in the Ukrainian population sample (part 1. age associations)","authors":"O. Filiptsova, O. Bashura, O. Naboka, Oleksandra Kran, M. Almakaiev, H. Kukhtenko, T. Martyniuk, Yuliia Osypenko","doi":"10.15587/2519-8025.2022.271043","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.271043","url":null,"abstract":"The aim. The study encompassed some aspects related to perfumes use, which can potentially influence the individual perfume choice, in particular, among different age groups of the Ukrainian population. Factors related to perfumes choice, the state of awareness of respondents regarding quality, safety, and existing stereotypes regarding the characteristics of perfumes and potential harmful effects on the human body were investigated. \u0000Materials and methods. The analysis was performed on a sample of 124 residents of Ukraine. Respondents, who were randomly selected, were offered a questionnaire, developed by us, which included 15 items on the demographic characteristics of the subjects and questions related to the perfume use of and stereotypes about them. The Pearson chi-squared (χ2) test was used to analyze the relationships between qualitative characteristics. \u0000Results and discussion. About 98 % of surveyed persons in Ukraine use perfumery. Associations were found between age and the probability of purchase by the study participants of non-original perfume products and awareness of the safety of various perfume products. It has been established, that the determining factors when choosing a perfume is a certain scent, while the brand is given secondary importance. An increase in the effect of the brand in older age groups of the population has been demonstrated. Behavioral features of perfume use have been established: in younger women, it is associated with increased self-esteem and the influence of social networks, with age, the focus shifts to the desire for a positive effect on the mood from the use of a certain fragrance. However, in all age groups, the main purpose of perfume using was to improve the emotional state. Middle-aged women are more democratic in choosing perfumery with a specific gender orientation. \u0000Conclusions. For the first time in Ukraine, a pilot study was conducted to find possible associations between some biological, behavioral and demographic characteristics and the perfume use. Such factors as age and education level are promising for further analysis of the selected issues","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84333808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.15587/2519-8025.2022.271011
A. Petrushko, N. Grushanska
The aim: To investigate the prevalence of cardiomyopathy (CMP) phenotypes and the weight of cardiogenic arterial thromboembolism (ATE) as a complication in cats. To determine the influence of age, breed and sex on the frequency of detection of CMP and ATE. Animals: One hundred and seventeen cats with various phenotypes of cardiomyopathies. Materials and methods: the database of the veterinary center was analyzed. From 1066 studied cats, 117 stories of animal diseases were selected, in which the diagnosis of cardiomyopathy was established. Results: In the experimental group, the prevalence of CMP was 11 % (HCMP - 94 %; DCMP - 3.4 %; RCMP - 2.6 %). Males were affected more often and had a more severe course. As the age of the animals increased, the relative frequency of heart failure increased slightly, but CMP was diagnosed more often in young animals. The Scottish Straight, Scottish Fold, European Shorthaired, European Longhaired, British Longhaired, and Canadian Sphynx can be considered susceptible breeds in Vinnytsia. There have been no cases of cardiomyopathy in Maine Coons. 57 % of cats with HCMP phenotype were asymptomatic. Arterial thromboembolism occurred in 14.5 % of animals with various CMP phenotypes. The lethality of ATE before discharge was 17.6 %, euthanized (different time period after the incident) 29 %. Conclusion: HCMP phenotype is the most common. Probably, genetic factors related to sex and breed have a significant influence on the development of cardiomyopathy. More often, CMP phenotypes were diagnosed in younger animals. Heart failure develops more often in older animals. The number of animals with ATE and HCMP phenotype is high, but relative to the number of animals with heart failure, cats with DCMP were more prone. ATE occurred more often in females. Thromboembolism is more common in the European Longhair breed.
{"title":"Prevalence of feline cardiomyopathy phenotypes and arterial thromboembolism","authors":"A. Petrushko, N. Grushanska","doi":"10.15587/2519-8025.2022.271011","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.271011","url":null,"abstract":"The aim: To investigate the prevalence of cardiomyopathy (CMP) phenotypes and the weight of cardiogenic arterial thromboembolism (ATE) as a complication in cats. To determine the influence of age, breed and sex on the frequency of detection of CMP and ATE. Animals: One hundred and seventeen cats with various phenotypes of cardiomyopathies. \u0000Materials and methods: the database of the veterinary center was analyzed. From 1066 studied cats, 117 stories of animal diseases were selected, in which the diagnosis of cardiomyopathy was established. \u0000Results: In the experimental group, the prevalence of CMP was 11 % (HCMP - 94 %; DCMP - 3.4 %; RCMP - 2.6 %). Males were affected more often and had a more severe course. As the age of the animals increased, the relative frequency of heart failure increased slightly, but CMP was diagnosed more often in young animals. The Scottish Straight, Scottish Fold, European Shorthaired, European Longhaired, British Longhaired, and Canadian Sphynx can be considered susceptible breeds in Vinnytsia. There have been no cases of cardiomyopathy in Maine Coons. 57 % of cats with HCMP phenotype were asymptomatic. Arterial thromboembolism occurred in 14.5 % of animals with various CMP phenotypes. The lethality of ATE before discharge was 17.6 %, euthanized (different time period after the incident) 29 %. \u0000Conclusion: HCMP phenotype is the most common. Probably, genetic factors related to sex and breed have a significant influence on the development of cardiomyopathy. More often, CMP phenotypes were diagnosed in younger animals. Heart failure develops more often in older animals. The number of animals with ATE and HCMP phenotype is high, but relative to the number of animals with heart failure, cats with DCMP were more prone. ATE occurred more often in females. Thromboembolism is more common in the European Longhair breed.","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"222 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88381631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15587/2519-8025.2022.266239
Andriy Berezovskiy, T. Fotina, Yevheniia Vashchyk, Dmytro Berezhnyi, D. Morozenko
The aim: study of the effectiveness of environmentally safe disinfectants against P. aeruginosa and pathogens of the main bacteriosis of poultry at test facilities. Materials and methods. To study the antimicrobial action of the investigated disinfectants against a mixture of epizootic cultures of E. coli, P. aeruginosa, S. aureus, S. typhimurium, isolated from poultry, bacteriological studies were carried out on test objects: galvanized iron, wooden bars (painted and unpainted), red brick, cutouts from plaster, size 10 × 10 cm. Results. The working solutions of the new disinfectant "Dezsan" were studied in comparison with the control agents: "Virocid" and "Bi-dez" at a concentration of 0.01; 0.1; 0.25; 0.5; 1; 1.5 % in relation to suspension cultures of E. coli, P. aeruginosa, S. aureus, S. typhimurium. In this case, it was established, that the "Dezsan" agent showed an antimicrobial effect on rough test objects after exposure for 3 hours at a concentration of 0.1 %, and at a concentration of 0.25 % - for 1 hour. On smooth surfaces, the agent neutralized bacterial cultures at a concentration of 0.1 % after exposure for 1 hour. The preparation "Bi-dez" at a concentration of 0.25 % was effective on smooth surfaces after exposure for 1 hour, on rough surfaces (brick, plaster) - at a concentration of 0.5 % after exposure for 3 hours or more. The working solution of 1 % concentration neutralized bacterial cultures on all types of surfaces after exposure for 1 hour or more. "Virocid" agent after exposure for 1 hour neutralized bacterial cultures on smooth surfaces in concentrations of 0.25 % and higher; on rough surfaces, the growth of cultures was not detected when using a 0.5 % solution. Conclusions. Environmentally safe disinfectants "Dezsan" and "Shumerske sryblo" compared to the control ones ("Bi Dez" and "Virotsid") show an active antimicrobial effect at a concentration of 0.25 % and 3 %, respectively, against the suspension of epizootic cultures of E. coli, P. aeruginosa, S. aureus and S. typhimurium on different types of production surfaces, which justifies the feasibility of their use based on the principle of rotation of disinfectants for the prevention of bacterial pseudomonosis of poultry
{"title":"Effectiveness of ecologically safe disinfectants against Pseudomonas aeruginosa and the poultry main bacteriosis pathogens","authors":"Andriy Berezovskiy, T. Fotina, Yevheniia Vashchyk, Dmytro Berezhnyi, D. Morozenko","doi":"10.15587/2519-8025.2022.266239","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.266239","url":null,"abstract":"The aim: study of the effectiveness of environmentally safe disinfectants against P. aeruginosa and pathogens of the main bacteriosis of poultry at test facilities. \u0000Materials and methods. To study the antimicrobial action of the investigated disinfectants against a mixture of epizootic cultures of E. coli, P. aeruginosa, S. aureus, S. typhimurium, isolated from poultry, bacteriological studies were carried out on test objects: galvanized iron, wooden bars (painted and unpainted), red brick, cutouts from plaster, size 10 × 10 cm. \u0000Results. The working solutions of the new disinfectant \"Dezsan\" were studied in comparison with the control agents: \"Virocid\" and \"Bi-dez\" at a concentration of 0.01; 0.1; 0.25; 0.5; 1; 1.5 % in relation to suspension cultures of E. coli, P. aeruginosa, S. aureus, S. typhimurium. In this case, it was established, that the \"Dezsan\" agent showed an antimicrobial effect on rough test objects after exposure for 3 hours at a concentration of 0.1 %, and at a concentration of 0.25 % - for 1 hour. On smooth surfaces, the agent neutralized bacterial cultures at a concentration of 0.1 % after exposure for 1 hour. The preparation \"Bi-dez\" at a concentration of 0.25 % was effective on smooth surfaces after exposure for 1 hour, on rough surfaces (brick, plaster) - at a concentration of 0.5 % after exposure for 3 hours or more. The working solution of 1 % concentration neutralized bacterial cultures on all types of surfaces after exposure for 1 hour or more. \"Virocid\" agent after exposure for 1 hour neutralized bacterial cultures on smooth surfaces in concentrations of 0.25 % and higher; on rough surfaces, the growth of cultures was not detected when using a 0.5 % solution. \u0000Conclusions. Environmentally safe disinfectants \"Dezsan\" and \"Shumerske sryblo\" compared to the control ones (\"Bi Dez\" and \"Virotsid\") show an active antimicrobial effect at a concentration of 0.25 % and 3 %, respectively, against the suspension of epizootic cultures of E. coli, P. aeruginosa, S. aureus and S. typhimurium on different types of production surfaces, which justifies the feasibility of their use based on the principle of rotation of disinfectants for the prevention of bacterial pseudomonosis of poultry","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86767021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15587/2519-8025.2022.266451
D. Morozenko, Yevheniia Vashchyk, A. Zakhariev, N. Seliukova, K. Gliebova, Dmytro Berezhnyi
The aim: to analyze the diagnostic informativeness of biochemical markers of connective tissue in the blood of dogs with babesiosis. Materials and methods. German Shepherd (n=7), Labrador Retriever (n=3) and Rottweiler (n=2) dogs aged 1 to 5 years and diagnosed with babesiosis were studied. As a control group, clinically healthy dogs were used, which came to the veterinary clinic for a preventive examination, the age of the animals was from 1 to 5 years (n=10). The animals were examined according to the following scheme: collection of anamnestic data, clinical examination according to generally accepted methods, general and biochemical blood analysis, urine examination. Glycoproteins in blood serum were determined according to Shteinberg – Dotsenko, sialic acids – according to the Hess method, chondroitin sulfates – Nemeth–Csoka in the modification of L.I. Slutsky. Results. The biochemical examination of the blood revealed the presence of acute cytolytic syndrome and cholestasis in the animal's body. Cholestasis in sick animals was characterized by an increase in the content of bilirubin and the activity of alkaline phosphatase, as well as an increase in the content of β-lipoproteins. The increase in the content of total bilirubin in the blood was obviously due mainly to its unconjugated fraction, since intravascular hemolysis took place. In the blood serum of patients with babesiosis in dogs, there was an increase in the content of markers of connective tissue metabolism – glycoproteins by 1.63, sialic acids – by 1.36, and chondroitin sulfates by 1.8 times, respectively. Conclusions. Glycoproteins have "acute phase proteins" in their composition, which are indicators for assessing the degree of the inflammatory process in the body of dogs with babesiosis. Sialic acids are components of sialoproteins, which are also markers of the inflammatory process and destructive changes in the body. An increase in the content of chondroitin sulfates in the blood during babesiosis indicates the development of a compensatory reaction, associated with the action of toxic hemolysis products on the endothelium of blood vessels. Thus, the increased content of biochemical markers of connective tissue in the blood of dogs with babesiosis indicates the presence of a systemic inflammatory-protective reaction in animals, which makes it possible to recommend them for assessing the acute phase of the inflammatory process in the body and protecting blood vessels from damage due to toxic hemolysis
{"title":"Informativeness of biochemical markers of connective tissue metabolism in canine babesiosis","authors":"D. Morozenko, Yevheniia Vashchyk, A. Zakhariev, N. Seliukova, K. Gliebova, Dmytro Berezhnyi","doi":"10.15587/2519-8025.2022.266451","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.266451","url":null,"abstract":"The aim: to analyze the diagnostic informativeness of biochemical markers of connective tissue in the blood of dogs with babesiosis. \u0000Materials and methods. German Shepherd (n=7), Labrador Retriever (n=3) and Rottweiler (n=2) dogs aged 1 to 5 years and diagnosed with babesiosis were studied. As a control group, clinically healthy dogs were used, which came to the veterinary clinic for a preventive examination, the age of the animals was from 1 to 5 years (n=10). The animals were examined according to the following scheme: collection of anamnestic data, clinical examination according to generally accepted methods, general and biochemical blood analysis, urine examination. Glycoproteins in blood serum were determined according to Shteinberg – Dotsenko, sialic acids – according to the Hess method, chondroitin sulfates – Nemeth–Csoka in the modification of L.I. Slutsky. \u0000Results. The biochemical examination of the blood revealed the presence of acute cytolytic syndrome and cholestasis in the animal's body. Cholestasis in sick animals was characterized by an increase in the content of bilirubin and the activity of alkaline phosphatase, as well as an increase in the content of β-lipoproteins. The increase in the content of total bilirubin in the blood was obviously due mainly to its unconjugated fraction, since intravascular hemolysis took place. In the blood serum of patients with babesiosis in dogs, there was an increase in the content of markers of connective tissue metabolism – glycoproteins by 1.63, sialic acids – by 1.36, and chondroitin sulfates by 1.8 times, respectively. \u0000Conclusions. Glycoproteins have \"acute phase proteins\" in their composition, which are indicators for assessing the degree of the inflammatory process in the body of dogs with babesiosis. Sialic acids are components of sialoproteins, which are also markers of the inflammatory process and destructive changes in the body. An increase in the content of chondroitin sulfates in the blood during babesiosis indicates the development of a compensatory reaction, associated with the action of toxic hemolysis products on the endothelium of blood vessels. Thus, the increased content of biochemical markers of connective tissue in the blood of dogs with babesiosis indicates the presence of a systemic inflammatory-protective reaction in animals, which makes it possible to recommend them for assessing the acute phase of the inflammatory process in the body and protecting blood vessels from damage due to toxic hemolysis","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89629880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.15587/2519-8025.2022.266240
D. Morozenko, Yevheniia Vashchykv, N. Kononenko, A. Zakhariev, N. Seliukova, Dmytro Berezhnyi, Gliebova Gliebova, V. Chikitkina
The aim: to analyze and establish the pathogenetic role of biochemical markers of the state of connective tissue in diseases of the stomach and intestines in dogs and cats. Materials and methods. The research was carried out by the method of analysis of sources of scientific literature (PubMed, Elsevier, electronic resources of the V. I. Vernadskyi National Library), because of which a scheme of the pathogenesis of gastroenteritis in dogs and cats with the participation of connective tissue biopolymers was created. Results. In dogs and cats, the issue of the use of biochemical markers for the diagnosis of diseases of the stomach and intestines has not yet been fully clarified. It is known, that in dogs and cats, lymphocytic-plasmacytic enteritis is histologically determined by fibrosis of the intestinal wall, but biochemical tests for the diagnosis of this condition are not given. Among the biochemical markers of inflammatory bowel disease, tumor necrosis factor, C-reactive protein and microalbumin were identified. Although C-reactive protein was elevated in a greater number of diseased animals, this increase was insignificant. Other tests also did not show high diagnostic informativeness. Several stages can be identified in the pathogenesis of alimentary gastroenteritis in dogs and cats. First, the irritating components of food act on the mucous membrane of the stomach and disrupt its secretory and motor functions, which causes gastritis. Thus, the use of indicators of the state of connective tissue in the diagnosis of intestinal diseases in dogs can be used to assess the degree of the inflammatory process. Conclusions. According to the results of the analysis, it was established, that the development of the inflammatory process in the stomach and intestines causes an increase in the content of glycoproteins in the blood serum of cats and dogs, and a decrease in synthetic processes in the liver is accompanied by a decrease in the concentration of glycosaminoglycans (GAG) in the blood serum of sick animals. It should be noted, that this decrease has peculiarities: in dogs, the content of total chondroitin sulfates remained at the level of clinically healthy animals, while the concentration of total GAG decreased. In cats, on the contrary, the content of total chondroitin sulfates decreased, and the fractional composition of GAG remained unchanged. The level of excretion of oxyproline and uronic acids in the urine of animals with gastroenteritis did not change, which indicates the absence of catabolism of collagen and proteoglycans with gastroenteritis
目的:分析和确立结缔组织状态生化标志物在犬、猫胃肠道疾病中的致病作用。材料和方法。研究通过对科学文献来源(PubMed, Elsevier, V. I. Vernadskyi国家图书馆电子资源)进行分析的方法进行，因此创建了结缔组织生物聚合物参与的狗和猫胃肠炎发病机制的方案。结果。在狗和猫中，使用生化标记物诊断肠胃疾病的问题尚未得到充分澄清。众所周知，在狗和猫中，淋巴细胞浆细胞性肠炎在组织学上是由肠壁纤维化决定的，但没有给出诊断这种情况的生化试验。炎症性肠病的生化指标包括肿瘤坏死因子、c反应蛋白和微量白蛋白。尽管c反应蛋白在更多患病动物中升高，但这种增加并不显著。其他测试也没有显示出很高的诊断信息。在狗和猫的消化道胃肠炎的发病机制中可以确定几个阶段。首先，食物中的刺激性成分作用于胃粘膜，破坏其分泌和运动功能，从而引起胃炎。因此，使用结缔组织状态指标诊断犬肠道疾病可用于评估炎症过程的程度。结论。根据分析结果，可以确定，胃和肠的炎症过程的发展导致猫和狗血清中糖蛋白含量的增加，而肝脏合成过程的减少伴随着患病动物血清中糖胺聚糖(GAG)浓度的降低。值得注意的是，这种下降具有特殊性:在狗中，总硫酸软骨素的含量保持在临床健康动物的水平，而总GAG的浓度下降。相反，在猫中，总硫酸软骨素的含量下降，GAG的分数组成保持不变。胃肠炎动物尿液中氧脯氨酸和糖醛酸的排泄水平没有变化，这表明胃肠炎动物的胶原蛋白和蛋白多糖的分解代谢缺失
{"title":"Biochemical markers of connective tissue in the pathogenesis of gastroenteritis in dogs and cats","authors":"D. Morozenko, Yevheniia Vashchykv, N. Kononenko, A. Zakhariev, N. Seliukova, Dmytro Berezhnyi, Gliebova Gliebova, V. Chikitkina","doi":"10.15587/2519-8025.2022.266240","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.266240","url":null,"abstract":"The aim: to analyze and establish the pathogenetic role of biochemical markers of the state of connective tissue in diseases of the stomach and intestines in dogs and cats. \u0000Materials and methods. The research was carried out by the method of analysis of sources of scientific literature (PubMed, Elsevier, electronic resources of the V. I. Vernadskyi National Library), because of which a scheme of the pathogenesis of gastroenteritis in dogs and cats with the participation of connective tissue biopolymers was created. \u0000Results. In dogs and cats, the issue of the use of biochemical markers for the diagnosis of diseases of the stomach and intestines has not yet been fully clarified. It is known, that in dogs and cats, lymphocytic-plasmacytic enteritis is histologically determined by fibrosis of the intestinal wall, but biochemical tests for the diagnosis of this condition are not given. Among the biochemical markers of inflammatory bowel disease, tumor necrosis factor, C-reactive protein and microalbumin were identified. Although C-reactive protein was elevated in a greater number of diseased animals, this increase was insignificant. Other tests also did not show high diagnostic informativeness. Several stages can be identified in the pathogenesis of alimentary gastroenteritis in dogs and cats. First, the irritating components of food act on the mucous membrane of the stomach and disrupt its secretory and motor functions, which causes gastritis. Thus, the use of indicators of the state of connective tissue in the diagnosis of intestinal diseases in dogs can be used to assess the degree of the inflammatory process. \u0000Conclusions. According to the results of the analysis, it was established, that the development of the inflammatory process in the stomach and intestines causes an increase in the content of glycoproteins in the blood serum of cats and dogs, and a decrease in synthetic processes in the liver is accompanied by a decrease in the concentration of glycosaminoglycans (GAG) in the blood serum of sick animals. It should be noted, that this decrease has peculiarities: in dogs, the content of total chondroitin sulfates remained at the level of clinically healthy animals, while the concentration of total GAG decreased. In cats, on the contrary, the content of total chondroitin sulfates decreased, and the fractional composition of GAG remained unchanged. The level of excretion of oxyproline and uronic acids in the urine of animals with gastroenteritis did not change, which indicates the absence of catabolism of collagen and proteoglycans with gastroenteritis","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87342873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-07DOI: 10.15587/2519-8025.2022.256113
V. Vdovin, S. Yarmoluk
The cytotoxic effect of several synthetic nitrogen-containing heterocyclic compounds on cultures of tumour and normal cells and the calculation of their ADME, QSAR, and DFT pharmacological properties The aim. The purpose of our work was to investigate the cytotoxic influence of some synthetic nitrogen-containing heterocyclic compounds, namely imidazole, aurones, and triazole on the culture of tumour cells of melanoma mouse B16, human glioma U251 and normal HEK293 and their ADME, QSAR, and DFT pharmacological properties calculation. Materials and methods. The estimation of cell viability in the conditions of influence of the investigated drugs was carried out by MTT. ADME data screening was performed by the SWISSADME server. QSAR calculations were performed on Way2Drug servers (cancerogenicity was predicted with ROSC-Pred, metabolism – with RA, side effects of drugs were investigated using AdverPred server, LD50 were predicted with Gusar software). The calculation of the functional density (DFT) was carried out using B3LYP and the functional of the exchange-correlation with the base set of 6-31 G (D, P) in the MMFF94 force field in the Avogadro program. The results. It was found that compounds 1 and 2 are toxic for normal cells HEK293, compounds 3, 4, 6 and 7 are low-toxic, and 5 does not inhibit cell growth at all. Our study has demonstrated that in the case of tumour cell line U251 compounds 2, 3 and 7 are non-toxic in general, and substances 1, 4, 5, 6 and 7 have significant toxicity. In a case of cancer cell line B16, compounds 1, 2, 4, 5, and 6 are toxic, and compound 7 is cytotoxic at any concentration. The test compounds (1–7) possess drug-like properties. All compounds meet Lipinski’s “rule of five” criteria. The BOILED-Egg model demonstrates that compound 3 may penetrate blood-brain barrier, all compounds except 1 can be absorbed in the intestine, 2 and 5 can be cleaved in the gastrointestinal tract and 3, 4, 6, and 7 have resistance to digestive enzymes. The analysis of metabolism showed that these compounds can mainly be metabolized by mechanisms of N- and O-glucuronidation and C-oxidation. The obtained data indicate that the smallest toxic effect is achieved with intravenously introduced compounds, and the largest toxicity is achieved with oral administration for compounds 3, 4, 5 and 6. The compounds 1 and 3 are completely noncarcinogenic, the other compounds can affect thyroid glands and hematopoietic system. This result requires further research when introduced into practical application. DFT calculations have shown that all investigated compounds are stable and reactive. Conclusions. Differences in the sensitivity of cell lines and dose-dependent effects of compounds detected during the study should be considered when calculating the optimal working concentrations of drugs. The results of the study are necessary to understand toxic effects on the cell lines B16, HEK293, and U251 and their further use for preclinical studies
几种合成含氮杂环化合物对肿瘤细胞和正常细胞的细胞毒作用及其ADME、QSAR和DFT药理性质的计算。我们的工作目的是研究合成含氮杂环化合物咪唑、aurones和三唑对黑色素瘤小鼠B16、人胶质瘤U251和正常HEK293肿瘤细胞培养的细胞毒性影响，并计算其ADME、QSAR和DFT药理学性质。材料和方法。用MTT法测定所研究药物影响条件下的细胞活力。ADME数据筛选由SWISSADME服务器执行。在Way2Drug服务器上进行QSAR计算(使用ROSC-Pred预测致癌性，使用代谢- RA，使用AdverPred服务器调查药物的副作用，使用Gusar软件预测LD50)。利用B3LYP和Avogadro程序中MMFF94力场中与6-31 G (D, P)基集交换相关的泛函进行了泛函密度(DFT)的计算。结果。发现化合物1、2对正常细胞HEK293有毒性，化合物3、4、6、7为低毒，化合物5对细胞生长无抑制作用。我们的研究表明，在肿瘤细胞系U251的情况下，化合物2、3和7一般是无毒的，物质1、4、5、6和7有明显的毒性。以癌细胞系B16为例，化合物1、2、4、5和6是有毒的，化合物7在任何浓度下都具有细胞毒性。测试化合物(1-7)具有类似药物的性质。所有化合物都符合利平斯基的“五法则”标准。煮蛋模型表明，化合物3可穿透血脑屏障，化合物1可在肠道内被吸收，化合物2和5可在胃肠道内被裂解，化合物3、4、6和7对消化酶具有抗性。代谢分析表明，这些化合物主要通过N-和o -葡萄糖醛酸化和c -氧化机制代谢。所获得的数据表明，静脉注射化合物的毒性作用最小，而口服化合物3、4、5和6的毒性作用最大。其中化合物1和3完全无致癌性，其他化合物可影响甲状腺和造血系统。这一结果在实际应用中有待进一步研究。DFT计算表明，所有被研究的化合物都是稳定的和活泼的。结论。在计算药物的最佳工作浓度时，应考虑细胞系的敏感性差异和研究中检测到的化合物的剂量依赖效应。该研究结果对于了解对细胞系B16、HEK293和U251的毒性作用及其在临床前研究中的进一步应用是必要的
{"title":"The cytotoxic effect of some synthetic nitrogen-containing heterocyclic compounds on cultures of tumour and normal cells and the calculation of their ADME, QSAR, and DFT pharmacological properties","authors":"V. Vdovin, S. Yarmoluk","doi":"10.15587/2519-8025.2022.256113","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.256113","url":null,"abstract":"The cytotoxic effect of several synthetic nitrogen-containing heterocyclic compounds on cultures of tumour and normal cells and the calculation of their ADME, QSAR, and DFT pharmacological properties \u0000The aim. The purpose of our work was to investigate the cytotoxic influence of some synthetic nitrogen-containing heterocyclic compounds, namely imidazole, aurones, and triazole on the culture of tumour cells of melanoma mouse B16, human glioma U251 and normal HEK293 and their ADME, QSAR, and DFT pharmacological properties calculation. \u0000Materials and methods. The estimation of cell viability in the conditions of influence of the investigated drugs was carried out by MTT. ADME data screening was performed by the SWISSADME server. QSAR calculations were performed on Way2Drug servers (cancerogenicity was predicted with ROSC-Pred, metabolism – with RA, side effects of drugs were investigated using AdverPred server, LD50 were predicted with Gusar software). The calculation of the functional density (DFT) was carried out using B3LYP and the functional of the exchange-correlation with the base set of 6-31 G (D, P) in the MMFF94 force field in the Avogadro program. \u0000The results. It was found that compounds 1 and 2 are toxic for normal cells HEK293, compounds 3, 4, 6 and 7 are low-toxic, and 5 does not inhibit cell growth at all. Our study has demonstrated that in the case of tumour cell line U251 compounds 2, 3 and 7 are non-toxic in general, and substances 1, 4, 5, 6 and 7 have significant toxicity. In a case of cancer cell line B16, compounds 1, 2, 4, 5, and 6 are toxic, and compound 7 is cytotoxic at any concentration. The test compounds (1–7) possess drug-like properties. All compounds meet Lipinski’s “rule of five” criteria. The BOILED-Egg model demonstrates that compound 3 may penetrate blood-brain barrier, all compounds except 1 can be absorbed in the intestine, 2 and 5 can be cleaved in the gastrointestinal tract and 3, 4, 6, and 7 have resistance to digestive enzymes. The analysis of metabolism showed that these compounds can mainly be metabolized by mechanisms of N- and O-glucuronidation and C-oxidation. The obtained data indicate that the smallest toxic effect is achieved with intravenously introduced compounds, and the largest toxicity is achieved with oral administration for compounds 3, 4, 5 and 6. The compounds 1 and 3 are completely noncarcinogenic, the other compounds can affect thyroid glands and hematopoietic system. This result requires further research when introduced into practical application. DFT calculations have shown that all investigated compounds are stable and reactive. \u0000Conclusions. Differences in the sensitivity of cell lines and dose-dependent effects of compounds detected during the study should be considered when calculating the optimal working concentrations of drugs. The results of the study are necessary to understand toxic effects on the cell lines B16, HEK293, and U251 and their further use for preclinical studies","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85965921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-07DOI: 10.15587/2519-8025.2022.254881
E. Domina
The aim. To conduct an analytical literature review on the possible impact of SARS-CoV-2 on the radiosensitivity of the human body and justify the relevance of radiobiological research in this area. Materials and methods. Analysis of data from biological dosimetry / indication of radiation lesions of human peripheral blood T-lymphocyte chromosomes under medical irradiation for comparison with radiosensitivity in the patients recovered from COVID-19 (Scopus International Scientific Metric Database, IAEA guidelines, 2011). Results. With the ongoing COVID-19 pandemic, forecasting and clarifying of the mechanisms of distant effects resulting from interactions between ionizing radiation and the SARS-CoV-2 virus play an important role. The difficulty in solving this problem is caused by the fact that the global science has no exhaustive information on the possible influence of this virus on radiation-induced effects. The attention of the professional community is drawn to the possible impact of SARS-CoV-2 on the radiosensitivity of the body of patients recovered from COVID-19 and a hypothesis is first proposed regarding the mechanism on how to increase it based on the development of systemic long-term inflammation. Therefore, clinical trials of low-dose radiotherapy for the treatment of COVID-19-related pneumonia involve preliminary radiobiological studies to answer the following question: does the SARS-CoV-2 virus affect the radiosensitivity of the human body? Long-term experience of the author of this paper in biodosimetric (cytogenetic) studies allows her to recommend the peripheral blood lymphocyte test system with chromosome aberration’s analysis as the most radiosensitive cell model. Conclusions. Clinical trials of low-dose radiotherapy for the treatment of COVID-19 pneumonia involve a preliminary radiobiological study to answer the following question: does the SARS-CoV-2 virus affect the radiosensitivity of the human body? The most optimal approach for the solution of this problem is the use of test-system of human peripheral blood lymphocytes’ culture with the subsequent cytogenetic analysis. It will allow investigating changes in the “dose-effect” “cell cycle stage-effect” dependencies, as well as changes in individual radiosensitivity under the influence of SARS-CoV-2 virus
{"title":"Possible effects of the exposure to ionizing radiation on the patients recovered from COVID-19","authors":"E. Domina","doi":"10.15587/2519-8025.2022.254881","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.254881","url":null,"abstract":"The aim. To conduct an analytical literature review on the possible impact of SARS-CoV-2 on the radiosensitivity of the human body and justify the relevance of radiobiological research in this area. \u0000Materials and methods. Analysis of data from biological dosimetry / indication of radiation lesions of human peripheral blood T-lymphocyte chromosomes under medical irradiation for comparison with radiosensitivity in the patients recovered from COVID-19 (Scopus International Scientific Metric Database, IAEA guidelines, 2011). \u0000Results. With the ongoing COVID-19 pandemic, forecasting and clarifying of the mechanisms of distant effects resulting from interactions between ionizing radiation and the SARS-CoV-2 virus play an important role. The difficulty in solving this problem is caused by the fact that the global science has no exhaustive information on the possible influence of this virus on radiation-induced effects. The attention of the professional community is drawn to the possible impact of SARS-CoV-2 on the radiosensitivity of the body of patients recovered from COVID-19 and a hypothesis is first proposed regarding the mechanism on how to increase it based on the development of systemic long-term inflammation. Therefore, clinical trials of low-dose radiotherapy for the treatment of COVID-19-related pneumonia involve preliminary radiobiological studies to answer the following question: does the SARS-CoV-2 virus affect the radiosensitivity of the human body? Long-term experience of the author of this paper in biodosimetric (cytogenetic) studies allows her to recommend the peripheral blood lymphocyte test system with chromosome aberration’s analysis as the most radiosensitive cell model. \u0000Conclusions. Clinical trials of low-dose radiotherapy for the treatment of COVID-19 pneumonia involve a preliminary radiobiological study to answer the following question: does the SARS-CoV-2 virus affect the radiosensitivity of the human body? The most optimal approach for the solution of this problem is the use of test-system of human peripheral blood lymphocytes’ culture with the subsequent cytogenetic analysis. It will allow investigating changes in the “dose-effect” “cell cycle stage-effect” dependencies, as well as changes in individual radiosensitivity under the influence of SARS-CoV-2 virus","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87564934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-07DOI: 10.15587/2519-8025.2022.255743
O. Starishko, T. Turytska, Anastasia Ovcharenko
The aim: to study changes in the microbiota of the urogenital tract of women of reproductive age in the Dnipro region, which is caused by conditionally pathogenic microorganisms on the background of smoking. Material and methods: biomaterial of patients (scraping from the urogenital tract) who applied to the Center for Laboratory Medicine PE “VIS-MEDIC” in Dnipro region. We analyzed the components of the microbiota of the vagina of women of the surveyed groups (sign of age and smoking) in the period from 2018 to 2021. For the use in an analysis, microbiota data were obtained using the test system Femoflor Screen. Results. Analysis of the results of the study revealed the dependence of the composition of the microbiota of the reproductive tract of women on the use of tobacco products. An increase in indicators for conditionally pathogenic microorganisms was shown. The rate of detection of elevated levels of M. hominis in samples of biological material varied between 6-8 % but was not recorded in all study groups of women. There was also an increase in the frequency of detection of elevated levels of U. urealyticum, U. parvum in samples of biological material, the values of which ranged from 9 to 50 %. Conclusions. The obtained data allowed to assess the effect of tobacco on the composition of the microbiota of the urogenital tract of women and made it possible to use them in measures of social and preventive work, as an indisputable fact to quit smoking. Therefore, there is a need for further research to establish the role of microorganisms involved in restoring the composition of the microbiota after inflammatory processes in women who use and do not use tobacco products. The results may be relevant for the diagnosis of inflammatory diseases, processes caused by opportunistic pathogens of the urogenital tract of women of reproductive age, potentially dangerous occurrence and development of infertility and the basis for social and preventive work among women on the background of smoking
{"title":"Urogenital infections of women of reproductive age caused by conditionally pathogenic microorganisms on the background of tobacco smoking","authors":"O. Starishko, T. Turytska, Anastasia Ovcharenko","doi":"10.15587/2519-8025.2022.255743","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.255743","url":null,"abstract":"The aim: to study changes in the microbiota of the urogenital tract of women of reproductive age in the Dnipro region, which is caused by conditionally pathogenic microorganisms on the background of smoking. \u0000Material and methods: biomaterial of patients (scraping from the urogenital tract) who applied to the Center for Laboratory Medicine PE “VIS-MEDIC” in Dnipro region. We analyzed the components of the microbiota of the vagina of women of the surveyed groups (sign of age and smoking) in the period from 2018 to 2021. For the use in an analysis, microbiota data were obtained using the test system Femoflor Screen. \u0000Results. Analysis of the results of the study revealed the dependence of the composition of the microbiota of the reproductive tract of women on the use of tobacco products. An increase in indicators for conditionally pathogenic microorganisms was shown. The rate of detection of elevated levels of M. hominis in samples of biological material varied between 6-8 % but was not recorded in all study groups of women. There was also an increase in the frequency of detection of elevated levels of U. urealyticum, U. parvum in samples of biological material, the values of which ranged from 9 to 50 %. \u0000Conclusions. The obtained data allowed to assess the effect of tobacco on the composition of the microbiota of the urogenital tract of women and made it possible to use them in measures of social and preventive work, as an indisputable fact to quit smoking. Therefore, there is a need for further research to establish the role of microorganisms involved in restoring the composition of the microbiota after inflammatory processes in women who use and do not use tobacco products. \u0000The results may be relevant for the diagnosis of inflammatory diseases, processes caused by opportunistic pathogens of the urogenital tract of women of reproductive age, potentially dangerous occurrence and development of infertility and the basis for social and preventive work among women on the background of smoking","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86008206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-07DOI: 10.15587/2519-8025.2022.255237
P. Liulin, M. Bogach, O. Getmanets
The aim. Identify the species of oocysts of turkeys by morphometric parameters. Material and methods. Eimeria oocysts obtained from faeces of suspects and patients with spontaneous eimeriosis of turkeys of poultry farms of Kharkiv region served as material for the research. Methods used: parasitological, coprological, light microscopy, morphometry, mathematical and statistical, correlation and regression analysis, ANOVA variation statistics. Results. Morphometrically (n=255) samples of turkey eimeria oocysts were studied and identified to the species: E. gallopavonis (n=50), E. meleagrimitis (n=50), E. adenoids (n=51), E. meleagridis n=53), E. innocua (n=51) according to identification indicators (X1 - length of the oocyst in μm; X2 - width of the oocyst in μm; X3 - area of the oocyst in μm; X4 - eccentricity of the model ellipse; X5 - ratio of the width of the oocyst to its length; X6 - largest curvature and X7 - smallest curvature in its model ellipse poles on the major and minor axes, respectively, in μm, X8 - presence - 1 or absence - 0 polar granules) which are mathematical expressions of morphometric dependences of the structure of eocystic oocysts which are confirmed by the results of regression and correlation analysis. The dependence of the Y oocyte species on seven characteristics has been proved. Conclusions. The morphological features that are mathematical expressions of morphometric dependences of the structure and identification of the species of turkey oocysts are determined with high accuracy. The relative error in determining the type of turkey eimerias does not exceed 2 %
{"title":"Identification of species of emeria of turkeys using regression analysis of morphometric indicators of ocycists","authors":"P. Liulin, M. Bogach, O. Getmanets","doi":"10.15587/2519-8025.2022.255237","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.255237","url":null,"abstract":"The aim. Identify the species of oocysts of turkeys by morphometric parameters. \u0000Material and methods. Eimeria oocysts obtained from faeces of suspects and patients with spontaneous eimeriosis of turkeys of poultry farms of Kharkiv region served as material for the research. Methods used: parasitological, coprological, light microscopy, morphometry, mathematical and statistical, correlation and regression analysis, ANOVA variation statistics. \u0000Results. Morphometrically (n=255) samples of turkey eimeria oocysts were studied and identified to the species: E. gallopavonis (n=50), E. meleagrimitis (n=50), E. adenoids (n=51), E. meleagridis n=53), E. innocua (n=51) according to identification indicators (X1 - length of the oocyst in μm; X2 - width of the oocyst in μm; X3 - area of the oocyst in μm; X4 - eccentricity of the model ellipse; X5 - ratio of the width of the oocyst to its length; X6 - largest curvature and X7 - smallest curvature in its model ellipse poles on the major and minor axes, respectively, in μm, X8 - presence - 1 or absence - 0 polar granules) which are mathematical expressions of morphometric dependences of the structure of eocystic oocysts which are confirmed by the results of regression and correlation analysis. The dependence of the Y oocyte species on seven characteristics has been proved. \u0000Conclusions. The morphological features that are mathematical expressions of morphometric dependences of the structure and identification of the species of turkey oocysts are determined with high accuracy. The relative error in determining the type of turkey eimerias does not exceed 2 %","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82745428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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