Pub Date : 2021-12-01DOI: 10.21608/sjdfs.2021.240753
eman Khalifaa, N. Omar
Forty adult male rats weighting (300±50) g were used and divided into 4 equal groups (10 rats/each); Group I: normal control rat group which received no treatment. Group ΙΙ: control rat treated orally with thyme leave extract only at a dose (500mg/kg b.w/d) for a month, Group ΙΙΙ: rat treated (i.p) with methotrexate (MTX) only at a dose (1mg/kg b.w/d) one per week for four weeks and dissolved in (2ml) of saline solution, Group ΙV: rats co-treated with methotrexate at a dose (1mg/kg b.w) and thyme extract at a dose (500mg/kg b.w) daily for a month at the same time. Thyme leave extract enhanced reproductive hormones (testosterone and estradiol E2) after inhibition of MTX. MTX induced increment in MDA level but thyme improved it. Antioxidants enzymes (GR, SOD and CAT) inhibited by MTX and thyme enhanced their depression. Moreover, HB, Platelets and WBCs recorded significant decrease in group of MTX but improved in MTX and thyme treatment group. In conclusion, thyme extract is more effective in the improvement of physiological abnormalities of testicular of rat after injection with MTX.
{"title":"Role of Methotrexate and Thyme in Physiological Changes of Male Rat","authors":"eman Khalifaa, N. Omar","doi":"10.21608/sjdfs.2021.240753","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.240753","url":null,"abstract":"Forty adult male rats weighting (300±50) g were used and divided into 4 equal groups (10 rats/each); Group I: normal control rat group which received no treatment. Group ΙΙ: control rat treated orally with thyme leave extract only at a dose (500mg/kg b.w/d) for a month, Group ΙΙΙ: rat treated (i.p) with methotrexate (MTX) only at a dose (1mg/kg b.w/d) one per week for four weeks and dissolved in (2ml) of saline solution, Group ΙV: rats co-treated with methotrexate at a dose (1mg/kg b.w) and thyme extract at a dose (500mg/kg b.w) daily for a month at the same time. Thyme leave extract enhanced reproductive hormones (testosterone and estradiol E2) after inhibition of MTX. MTX induced increment in MDA level but thyme improved it. Antioxidants enzymes (GR, SOD and CAT) inhibited by MTX and thyme enhanced their depression. Moreover, HB, Platelets and WBCs recorded significant decrease in group of MTX but improved in MTX and thyme treatment group. In conclusion, thyme extract is more effective in the improvement of physiological abnormalities of testicular of rat after injection with MTX.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90277486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/sjdfs.2021.239486
May Elzanatey, Amoura Abou ElNaga, H. El-Gammal, M. El-Zayat
Many sperm preparation procedures are used in intracytoplasmic sperm injection (ICSI), including migration, density gradient centrifugation, and filtering. Magnetic-activated cell sorting (MACS) is a type of magnetic separation that has been used in a variety of biomedical and biological studies. To examine the fertilisation rate, cleavage, and development of Blastocystes in two sperm preparation procedures for ICSI, MACS and density gradient centrifugation (DGC). The research was carried out in the Royal Center in Mansoura, Dakahlia Governorate, Egypt. Fifty patients receiving ICSI had their sperm samples taken. The sperm were separated into two groups using traditional DGC. One group served as DGC, while the other served as MACS. The number of oocytes harvested, the number of metaphase II oocytes injected, fertilisation rates, cleavage rates, and embryo quality were all compared. between MACS and groups. quality, statistically significant difference between the two groups in terms of the of quality and of MII a statistically significant negative connection. in the DGC group, a statistically significant negative connection between sperm DNA fragmentation (SDF) and fertilisation rate. MACS sperm selection of human spermatozoa is a safe, straightforward, and effective procedure for sperm preparation in a clinical environment for ICSI..
{"title":"EFFECTS OF MAGNETIC-ACTIVATED SPERM CELL SORTING TECHNIQUE IN BLASTOCYST FORMATION AFTER INTRACYTOPLASMIC SPERM INJECTION","authors":"May Elzanatey, Amoura Abou ElNaga, H. El-Gammal, M. El-Zayat","doi":"10.21608/sjdfs.2021.239486","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.239486","url":null,"abstract":"Many sperm preparation procedures are used in intracytoplasmic sperm injection (ICSI), including migration, density gradient centrifugation, and filtering. Magnetic-activated cell sorting (MACS) is a type of magnetic separation that has been used in a variety of biomedical and biological studies. To examine the fertilisation rate, cleavage, and development of Blastocystes in two sperm preparation procedures for ICSI, MACS and density gradient centrifugation (DGC). The research was carried out in the Royal Center in Mansoura, Dakahlia Governorate, Egypt. Fifty patients receiving ICSI had their sperm samples taken. The sperm were separated into two groups using traditional DGC. One group served as DGC, while the other served as MACS. The number of oocytes harvested, the number of metaphase II oocytes injected, fertilisation rates, cleavage rates, and embryo quality were all compared. between MACS and groups. quality, statistically significant difference between the two groups in terms of the of quality and of MII a statistically significant negative connection. in the DGC group, a statistically significant negative connection between sperm DNA fragmentation (SDF) and fertilisation rate. MACS sperm selection of human spermatozoa is a safe, straightforward, and effective procedure for sperm preparation in a clinical environment for ICSI..","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"2014 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86600513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/sjdfs.2021.209357
R. Zahran, L. El-Sayed, S. Ayyad
{"title":"Impact of Ottelione on Gamma-glutamyl transferase and Hematological markers in mice with Liver Fibrosis","authors":"R. Zahran, L. El-Sayed, S. Ayyad","doi":"10.21608/sjdfs.2021.209357","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.209357","url":null,"abstract":"","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76772885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.21608/sjdfs.2021.195589
N. Hassan, H. Ebeed, H. S. Ahmed
Senescence is a genetically regulated process that involves decomposition of cellular structures and the mobilization of the products to other plant parts. The progression of developmental senescence in wheat plant under the effect of foliar application with 100 µM of the polyamine spermine (Spm) was studied. Biochemical changes were recorded in the fourth leaf starting from the onset of senescence at 30 day after sowing (DAS) until the end of leaf senescence at 50 DAS. Leaf senescence was delayed in Spm-treated plants through maintaining water content, preventing chlorophyll degradation and enhancement of total soluble sugars, starch accumulation and total protein compared to controls. In addition, reactive oxygen species and lipid peroxidation were lower in Spm-treated plants than control values. Catalase (CAT) activity was obviously lowered due to Spm-treatment; however, guaiacol peroxidase activity was slightly decreased below the control
{"title":"Exogenous Application of the Polyamine Spermine Delays Natural Leaf Senescence in Wheat Through Protecting Chlorophyll from Degradation and Preventing Oxidative Stress","authors":"N. Hassan, H. Ebeed, H. S. Ahmed","doi":"10.21608/sjdfs.2021.195589","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.195589","url":null,"abstract":"Senescence is a genetically regulated process that involves decomposition of cellular structures and the mobilization of the products to other plant parts. The progression of developmental senescence in wheat plant under the effect of foliar application with 100 µM of the polyamine spermine (Spm) was studied. Biochemical changes were recorded in the fourth leaf starting from the onset of senescence at 30 day after sowing (DAS) until the end of leaf senescence at 50 DAS. Leaf senescence was delayed in Spm-treated plants through maintaining water content, preventing chlorophyll degradation and enhancement of total soluble sugars, starch accumulation and total protein compared to controls. In addition, reactive oxygen species and lipid peroxidation were lower in Spm-treated plants than control values. Catalase (CAT) activity was obviously lowered due to Spm-treatment; however, guaiacol peroxidase activity was slightly decreased below the control","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77662746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.21608/sjdfs.2021.195582
S. Ahmed, M. Deyab, Radwa S. El-Shanawany, M. El-Adl
This study aims to compare between the marine alga Chaetomorpha vieillardi and the freshwater alga Cladophora glomerata. Water samples and massive growth of two species were collected from Port Said marine water pond and Ras El-Bar freshwater pond, respectively in 2019. The highly massive growth of Ch. vieillardi and C. glomerata, due to the trophic status and optimal physico-chemical properties of their habitats, causes ecological and economic problems. The very high massive growth of two species leads to relatively complete elimination of dissolved oxygen (DO) at the middle and bottom level of water during the second half of the night. Loss of DO values led to great loss of aquaculture. Although both algal species were belonging to the same family, yet they prefer different habitats where, Ch. vieillardi formed bloom in marine water but C. glomerata in freshwater. The significant variation in the biochemical, phytochemical compositions and heavy metal bioaccumulation capacity of Ch. vieillardi and C. glomerata could be attributed to the variation in biological properties of two species and the physicochemical characteristics of their habitats. Ch. vieillardi has higher carbohydrate and lipid content compared to C. glomerata. Most heavy metals accumulated in C. glomerata was more than that in Ch. vieillardi, but Cu and Fe were highly accumulated in Ch. vieillardi.
{"title":"Comparative Studies on Cladophora Glomerata and Chaetomorpha Vieillardi (Cladophoraceae) at North Delta-Egypt","authors":"S. Ahmed, M. Deyab, Radwa S. El-Shanawany, M. El-Adl","doi":"10.21608/sjdfs.2021.195582","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.195582","url":null,"abstract":"This study aims to compare between the marine alga Chaetomorpha vieillardi and the freshwater alga Cladophora glomerata. Water samples and massive growth of two species were collected from Port Said marine water pond and Ras El-Bar freshwater pond, respectively in 2019. The highly massive growth of Ch. vieillardi and C. glomerata, due to the trophic status and optimal physico-chemical properties of their habitats, causes ecological and economic problems. The very high massive growth of two species leads to relatively complete elimination of dissolved oxygen (DO) at the middle and bottom level of water during the second half of the night. Loss of DO values led to great loss of aquaculture. Although both algal species were belonging to the same family, yet they prefer different habitats where, Ch. vieillardi formed bloom in marine water but C. glomerata in freshwater. The significant variation in the biochemical, phytochemical compositions and heavy metal bioaccumulation capacity of Ch. vieillardi and C. glomerata could be attributed to the variation in biological properties of two species and the physicochemical characteristics of their habitats. Ch. vieillardi has higher carbohydrate and lipid content compared to C. glomerata. Most heavy metals accumulated in C. glomerata was more than that in Ch. vieillardi, but Cu and Fe were highly accumulated in Ch. vieillardi.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85601951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.21608/sjdfs.2021.195585
S. Ahmed, M. Deyab, Fatma S. El-Ashry, M. El-Adl
Macroalgae produce a wide variety of chemically active secondary metabolites. Brown seaweed; Sargassum vulgare and its associated seaweeds were collected from Hurghada Red sea coast of Egypt. Optimal physicochemical properties; slightly alkaline; low turbidity, moderate temperature and available nutrient content of saline water produced massive growth of S. vulgare (53% covering percentage) during autumn (2018). Heavy metals accumulation inside the investigated seaweed S. vulgare was within the usually range. The associated macroalgal species with S. vulgare are belonging to 9 genuses, 12 species. Chromatography, mass spectrophotometry (GC/MS) analysis revealed the presence of Twenty five bioactive compounds. The major phytochemical constituents in the chloroform extract of S. vulgare are n-Hexadecanoic acid 28.29 %, Heptacosane 8.04 %, trans-13-Octadecenoic acid 5.50 %, Oleic Acid 4.24 %, Palmitoleic acid 3.56 % and Hexadecanoic acid, ethyl ester 3.30 %.
{"title":"Qualitative and Quantitative Phytochemical Composition of Sargassum Vulgare at Hurghada Red Sea Coast - Egypt","authors":"S. Ahmed, M. Deyab, Fatma S. El-Ashry, M. El-Adl","doi":"10.21608/sjdfs.2021.195585","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.195585","url":null,"abstract":"Macroalgae produce a wide variety of chemically active secondary metabolites. Brown seaweed; Sargassum vulgare and its associated seaweeds were collected from Hurghada Red sea coast of Egypt. Optimal physicochemical properties; slightly alkaline; low turbidity, moderate temperature and available nutrient content of saline water produced massive growth of S. vulgare (53% covering percentage) during autumn (2018). Heavy metals accumulation inside the investigated seaweed S. vulgare was within the usually range. The associated macroalgal species with S. vulgare are belonging to 9 genuses, 12 species. Chromatography, mass spectrophotometry (GC/MS) analysis revealed the presence of Twenty five bioactive compounds. The major phytochemical constituents in the chloroform extract of S. vulgare are n-Hexadecanoic acid 28.29 %, Heptacosane 8.04 %, trans-13-Octadecenoic acid 5.50 %, Oleic Acid 4.24 %, Palmitoleic acid 3.56 % and Hexadecanoic acid, ethyl ester 3.30 %.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80093387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.21608/sjdfs.2021.195590
S. Ahmed, M. Deyab, Amira A.M. Al Afefy, M. El-Adl
The high trophic status of brackish water and sediments at the Deltaic coast, especially the high nitrogen, phosphorus contents, along with relatively low pH and water temperature resulted in overgrowth of Ulva pertusa at El Garabaa and Ulva lactuca at Damietta river Nile Estuary. This represents an environmental problem of aquatic environment and water quality. On the other hand, this massive growth of Ulva species can be used as economic source of different natural products. The biochemical analysis of Ulva species indicated that Ulva pertusa have higher contents of proteins, lipids, minerals, soluble and insoluble carbohydrates than those of Ulva lactuca. The qualitative studies of natural products indicated that Ulva pertusa contains alkaloids, saponins, flavonoids, phenols and quinones while Ulva lactuca contains alkaloids, steroids, flavonoids, cumarins and quinones. Further study to determine the quantitative contents of natural products and its bioactivity is needed.
{"title":"Ulva species Blooms and its Biochemical Composition in Relation to Aquatic Environmental Properties at North Damietta- Egypt","authors":"S. Ahmed, M. Deyab, Amira A.M. Al Afefy, M. El-Adl","doi":"10.21608/sjdfs.2021.195590","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.195590","url":null,"abstract":"The high trophic status of brackish water and sediments at the Deltaic coast, especially the high nitrogen, phosphorus contents, along with relatively low pH and water temperature resulted in overgrowth of Ulva pertusa at El Garabaa and Ulva lactuca at Damietta river Nile Estuary. This represents an environmental problem of aquatic environment and water quality. On the other hand, this massive growth of Ulva species can be used as economic source of different natural products. The biochemical analysis of Ulva species indicated that Ulva pertusa have higher contents of proteins, lipids, minerals, soluble and insoluble carbohydrates than those of Ulva lactuca. The qualitative studies of natural products indicated that Ulva pertusa contains alkaloids, saponins, flavonoids, phenols and quinones while Ulva lactuca contains alkaloids, steroids, flavonoids, cumarins and quinones. Further study to determine the quantitative contents of natural products and its bioactivity is needed.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89223202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.21608/sjdfs.2021.195593
M. Deyab, M. El-sheekh, R. Hasan, A. Elsadany, S. Ahmed
Anabaena variabilis was collected by isolation from saline soil at Elhamol city, Kafr Elsheikh governerate, Egypt. Spirulina platensis was obtained from cyanobacteria Lab., Microbiology Dept., Sakha Agricultural Research Station, Egypt. GC/MS analysis showed that 37 and 7 bioactive compounds from A. variabilis and S. platensis were identified, respectively. Moreover, the area percentage of the same bioactive compound varied according to the type of cyanobacteria. The major phytochemical constituents in the methanol extract of A. variabilis are Pentadecanoic acid 29%, 12,15-Octadecadienoic acid methyl ester 10.26%, Hexadecenoic acid methyl ester (Z) 8.3%, Thieno [3,4-C] pyridine,1,3,4,7-tetraphenyl 5.86%. While, S. platensis methanol extract contain Hexadecanoic acid 29%, 9,12,15-Octadecadienoic acid methyl ester 24% and 9,12 Octadecatrienoic acid zz methyl ester, 24.36%.
{"title":"Phytochemical Components of Two Cyanobacterial Local Strains","authors":"M. Deyab, M. El-sheekh, R. Hasan, A. Elsadany, S. Ahmed","doi":"10.21608/sjdfs.2021.195593","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.195593","url":null,"abstract":"Anabaena variabilis was collected by isolation from saline soil at Elhamol city, Kafr Elsheikh governerate, Egypt. Spirulina platensis was obtained from cyanobacteria Lab., Microbiology Dept., Sakha Agricultural Research Station, Egypt. GC/MS analysis showed that 37 and 7 bioactive compounds from A. variabilis and S. platensis were identified, respectively. Moreover, the area percentage of the same bioactive compound varied according to the type of cyanobacteria. The major phytochemical constituents in the methanol extract of A. variabilis are Pentadecanoic acid 29%, 12,15-Octadecadienoic acid methyl ester 10.26%, Hexadecenoic acid methyl ester (Z) 8.3%, Thieno [3,4-C] pyridine,1,3,4,7-tetraphenyl 5.86%. While, S. platensis methanol extract contain Hexadecanoic acid 29%, 9,12,15-Octadecadienoic acid methyl ester 24% and 9,12 Octadecatrienoic acid zz methyl ester, 24.36%.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79038289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.21608/sjdfs.2021.195591
N. Elarabany, N. Omar, Mostafa M. Elshaer, A. AbdelKhalek, Maitha Rashed
The combination therapy has been the standard treatment for chronic hepatitis C virus (HCV) infection, but many patients have side effects due to this therapy. Recently, new direct-acting antivirals (DAAs) have enhanced the response rate, especially in difficult-to-treat patients infected with HCV genotypes (GT) 1 or 4, and have made adverse effects less common. RBV plays continually an important role in HCV therapy even with the introduction of DAAs. Hemolytic anemia is a common side effect in HCV-infected patients on combination therapy, affecting up to 30 % of patients, which needs to followup hemoglobin and dose modification in up to 15 % of patients. RBV-induced anemia primarily results in the reduction of adenosine triphosphate (ATP) levels in erythrocytes, affecting ATP-dependent oxidative metabolism. Genetic variations in the inosine triphosphatase (ITPA ) gene, which encodes an inosine triphosphate pyrophosphohydrolase (ITPase), protect from hemolytic anemia during dual therapy. These ITPA variants affect ITPase functionality, causing a drop in its activity, resulting in an accumulation of inosine triphosphate (ITP) in erythrocytes and the prevention of oxidative stress. The functional variants in ITPA gene leads to ITPase deficiency and a strong accumulation of ITP in erythrocytes, which is associated with lower RBV-toxicity. These genetic variants have been associated with protection from anemia.
{"title":"Association of ITPA Gene Expression and Hematological Abnormalities Induced by Dual therapy in HCV-infected Patients","authors":"N. Elarabany, N. Omar, Mostafa M. Elshaer, A. AbdelKhalek, Maitha Rashed","doi":"10.21608/sjdfs.2021.195591","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.195591","url":null,"abstract":"The combination therapy has been the standard treatment for chronic hepatitis C virus (HCV) infection, but many patients have side effects due to this therapy. Recently, new direct-acting antivirals (DAAs) have enhanced the response rate, especially in difficult-to-treat patients infected with HCV genotypes (GT) 1 or 4, and have made adverse effects less common. RBV plays continually an important role in HCV therapy even with the introduction of DAAs. Hemolytic anemia is a common side effect in HCV-infected patients on combination therapy, affecting up to 30 % of patients, which needs to followup hemoglobin and dose modification in up to 15 % of patients. RBV-induced anemia primarily results in the reduction of adenosine triphosphate (ATP) levels in erythrocytes, affecting ATP-dependent oxidative metabolism. Genetic variations in the inosine triphosphatase (ITPA ) gene, which encodes an inosine triphosphate pyrophosphohydrolase (ITPase), protect from hemolytic anemia during dual therapy. These ITPA variants affect ITPase functionality, causing a drop in its activity, resulting in an accumulation of inosine triphosphate (ITP) in erythrocytes and the prevention of oxidative stress. The functional variants in ITPA gene leads to ITPase deficiency and a strong accumulation of ITP in erythrocytes, which is associated with lower RBV-toxicity. These genetic variants have been associated with protection from anemia.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90497579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.21608/sjdfs.2021.195596
F. El-Taweel, Sara El-helf, M. Helal, Hatem El-mezayn
Hepatocellular carcinoma (HCC) is one of the most common types of cancer and its high mortality and short survival time causes a serious worldwide health burden. Chemotherapy as 5-Flourouracil (5-FU) is used as the first-choice treatment for HCC, however it is associated with resistance, low efficacy and has side effects. Consequently, the administration of mesenchymal stem cells (MSCs) as a novel therapy for HCC holds great promise. MSCs can differentiate into hepatocytes, reduce liver inflammation, promote hepatic regeneration and secrete protective cytokines. This is the first study that sheds new light on the role of MSCs alone or together with 5-FU in HCC. In this study, Bone marrow derived-MSCs were prepared and HCC model was induced via treating rats with N-diethylnitrosamine (DEN). The effect of treatments was evaluated by biochemical, tumor markers, macroscopical, and histopathological analysis. Also, cell cycle, apoptosis assay, KI67 and CD95 were performed by Flowcytometry. The administration of MSCs together with 5-FU yields a decrease in the liver enzymes with down-regulation of tumor markers as well as improvement of histopathological picture. In addition, such treatment decreased cell proliferation that associated with the down-regulation of KI67 expression. Furthermore, MSCs did not mediate the apoptosis pathway together with reduction of CD95 expression and cell cycle arrest at G0/G1 phase. In conclusion, MSCs exerted antitumor effects by inhibiting proliferation, modulating the cell cycle in G0/G1 phase and improved the toxic effect of 5FU via improvement of liver enzymes and decreased tumor markers.
{"title":"Possible Therapeutic Role of Bone Marrow-Derived Mesenchymal Stem Cells in a Rat Model of Hepatocellular Carcinoma","authors":"F. El-Taweel, Sara El-helf, M. Helal, Hatem El-mezayn","doi":"10.21608/sjdfs.2021.195596","DOIUrl":"https://doi.org/10.21608/sjdfs.2021.195596","url":null,"abstract":"Hepatocellular carcinoma (HCC) is one of the most common types of cancer and its high mortality and short survival time causes a serious worldwide health burden. Chemotherapy as 5-Flourouracil (5-FU) is used as the first-choice treatment for HCC, however it is associated with resistance, low efficacy and has side effects. Consequently, the administration of mesenchymal stem cells (MSCs) as a novel therapy for HCC holds great promise. MSCs can differentiate into hepatocytes, reduce liver inflammation, promote hepatic regeneration and secrete protective cytokines. This is the first study that sheds new light on the role of MSCs alone or together with 5-FU in HCC. In this study, Bone marrow derived-MSCs were prepared and HCC model was induced via treating rats with N-diethylnitrosamine (DEN). The effect of treatments was evaluated by biochemical, tumor markers, macroscopical, and histopathological analysis. Also, cell cycle, apoptosis assay, KI67 and CD95 were performed by Flowcytometry. The administration of MSCs together with 5-FU yields a decrease in the liver enzymes with down-regulation of tumor markers as well as improvement of histopathological picture. In addition, such treatment decreased cell proliferation that associated with the down-regulation of KI67 expression. Furthermore, MSCs did not mediate the apoptosis pathway together with reduction of CD95 expression and cell cycle arrest at G0/G1 phase. In conclusion, MSCs exerted antitumor effects by inhibiting proliferation, modulating the cell cycle in G0/G1 phase and improved the toxic effect of 5FU via improvement of liver enzymes and decreased tumor markers.","PeriodicalId":21655,"journal":{"name":"Scientific Journal for Damietta Faculty of Science","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80105372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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