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Antioxidants-based scores to predict Hepatocellular Carcinoma Patients among Chronic Hepatitis C individuals: correlation with Non-invasive Markers of Hepatic Fibrosis 基于抗氧化剂的评分预测慢性丙型肝炎患者的肝细胞癌:与肝纤维化非侵入性标志物的相关性
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.269829
E. Toson, G. Shiha, Enja Elfar
Background High prevalence of Hepatitis C virus (HCV) in Egypt considers it as the major cause of hepatocellular carcinoma (HCC). So, several attempts have been used to predict HCC but still need more. In this regard, the predicting abilities of superoxide dismutase (SOD) and catalase (CAT) activities as well as total antioxidant capacity (TAC) were tested for estimating chronic hepatitis C (CHC)-mediated HCC. Patients and methods Blood samples from 79 patients (23 CHC and 56 HCC) were withdrawn to estimate these antioxidants and the routine analysis markers. Beside 15 healthy volunteers were included Results The medians of SOD activity and TAC values were significantly elevated in sera of HCC patients with positive correlation (P<0.0001, r=0.334) when compared with those of either CHC or healthy controls (P<0.0001). On contrary, the median of CAT activity was significantly lowered (P<0.0001) with negative correlation with SOD activity (r=-0.146, P<0.04). Stepwise logistic regression analysis of the last three markers produced a score; named "Antioxidants-diagnostic" which increased the AUCs values and their diagnostic activities (P<0.0001). By combining the three marker values with that of Age-platelet index (API) produced a score named "Antioxidants-API". Also, by combining them with that of the routine analysis values produced a score named “AAI-antioxidants”. In each case the AUCs values were enhanced when compared with those of its native antioxidants (P<0.0001). Conclusion The assessment of these antioxidant activities and their combination in the last three scores can enhance their activities to more precisely predict HCC patients among CHC ones and control individuals.
背景:在埃及,丙型肝炎病毒(HCV)的高患病率被认为是导致肝细胞癌(HCC)的主要原因。因此,已经进行了一些预测HCC的尝试,但仍需要更多的尝试。在这方面,我们测试了超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性以及总抗氧化能力(TAC)的预测能力,以估计慢性丙型肝炎(CHC)介导的HCC。患者与方法79例(CHC 23例,HCC 56例)患者抽取血液,测定抗氧化剂及常规分析指标。结果HCC患者血清SOD活性和TAC值中位数与CHC及健康对照组相比均显著升高(P<0.0001, r=0.334),两者呈正相关(P<0.0001)。CAT活性中位数显著降低(P<0.0001),与SOD活性呈负相关(r=-0.146, P<0.04)。逐步logistic回归分析后三个标记产生得分;命名为“抗氧化剂-诊断”,提高了auc值和诊断活性(P<0.0001)。将3个标记值与年龄-血小板指数(age -血小板指数,API)相结合,得出“抗氧化剂-API”评分。同时,通过将它们与常规分析值相结合,产生了一个名为“aai -抗氧化剂”的分数。在每种情况下,与天然抗氧化剂相比,aus值都有所提高(P<0.0001)。结论评价这些抗氧化活性及其后3个评分的组合可提高其活性,更准确地预测CHC患者和对照个体的HCC患者。
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引用次数: 0
Synthesis of some new bis(pyrazol-5-ols), pyridones and benzo-[f]-thiochromene-2-carbonitrile derivatives bearing N-(4-chlorophenyl)-2-(4-formylphenoxy)acetamide moiety 一些新的双(吡唑-5-醇)、吡啶酮和含N-(4-氯苯基)-2-(4-甲酰苯氧基)乙酰胺的苯并[f]-硫代铬-2-碳腈衍生物的合成
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.269870
Mahmoud Arafat, Ehab Khalifa, F. El‐Taweel, S. Ayyad, K. Mohamed
Novel 4,4′-(arylmethylene)-bis(3-methyl-1-phenyl-1 H -pyrazol-5-ol) derivative 2 was synthesized via interaction of N -(4-chlorophenyl)-2-(4-formylphenoxy)acetamide ( 1 ) with diverse available reagent (two mole from 3-methyl-1H-pyrazol-5(4 H )-one). Moreover, One-pot pseudo three-component reaction of hydrazine hydrate, ethyl acetoacetate and aldehydes in ethanole using pipridine at 70°C afforded the corresponding aminopyrazole derivative 3 . on the other hand, cyanoacetamide scaffolds 4a,b was reacted with aromatic aldehyde particularly N -(4-chlorophenyl)-2-(4-formylphenoxy)acetamide ( 1 ), to afford arylidenes 5a,b that undergoes cyclization by heating in ethanol containing drops of piperidine as catalyst, and malononitrile afforded the corresponding pyridinone derivatives 6a,b . All freshly synthesized scaffolds were elucidated by considering the data of both elemental and spectral analyses.
通过N -(4-氯苯基)-2-(4-甲酰基苯氧基)乙酰胺(1)与多种可用试剂(3-甲基- 1h -吡唑-5(4 H)- 1两摩尔)的相互作用,合成了新的4,4′-(芳基亚甲基)-双(3-甲基-1-苯基-1H-吡唑-5-醇)衍生物2。在70℃条件下,用哌啶在乙醇中对水合肼、乙酰乙酸乙酯和醛类进行一锅伪三组分反应,得到相应的氨基吡唑衍生物3。另一方面,氰乙酰胺支架4a,b与芳香醛特别是N -(4-氯苯基)-2-(4-甲酰基苯氧基)乙酰胺(1)反应生成芳基烯5a,b,在含哌啶滴剂的乙醇中加热生成环化,丙二腈生成相应的吡啶酮衍生物6a,b。通过元素分析和光谱分析对新合成的支架进行了鉴定。
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引用次数: 0
Evaluating the effect of Moringa peregrina (Forssk.) Fiori seeds on some biochemical and oxidative stress markers of obese experimental rats. 辣木(Moringa peregrina)的药效评价鸢尾种子对肥胖实验大鼠一些生化和氧化应激标志物的影响。
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.269862
N. Elarabany, A. Hamad, Sanad Elsobaeiy
Obesity is a primary reason for morbidity and mortality. The current study aimed to evaluate the anti-obesity and antioxidant effect of Moringa peregrina (MP) seeds oil in obesity-induced rats. Forty male Sprague Dawley rats were divided into four equal groups of 10 rats each. Group C (control); group OB rats were fed a high-fat diet (HFD). Group MPI: rats treated with HFD+ MP seeds oil (250 mg/kg b.wt./day, orally) and group MPII. rats treated with HFD + MP seeds oil (500 mg/kg b.wt./day, orally). Blood samples were collected by the end of the experiment for determination of serum total cholesterol (TC), Triacylglycerols (TAG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL), and liver functions (ALT, AST, ALP). Moreover, malondialdehyde level (MDA), superoxide dismutase and catalase activity were measured in both plasma and liver. The results showed that TC, TAG, LDL, ALP, AST, ALT and MDA were significantly higher in obese rats than in the control group. On the other hand, administration of MP seeds oil to HFD-fed rats improved oxidative stress and lipid metabolism abnormalities by ameliorating TC, TAG and liver enzyme activities. These results suggest that MP seeds oil effectively enhances obesity-associated parameters and decreases its related complications.
肥胖是发病率和死亡率的主要原因。本研究旨在探讨辣木籽油对肥胖大鼠的抗肥胖和抗氧化作用。40只雄性斯普拉格·道利大鼠被分成4组,每组10只。C组(对照组);OB组大鼠饲喂高脂饲料(HFD)。MPI组:HFD+ MP籽油(250 mg/kg b.wt)处理大鼠;/天,口服)和MPII组。用HFD + MP籽油(500 mg/kg b.wt)处理大鼠。/天,口头)。实验结束时采集血液,测定血清总胆固醇(TC)、甘油三酯(TAG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和肝功能(ALT、AST、ALP)。测定血浆和肝脏丙二醛(MDA)水平、超氧化物歧化酶和过氧化氢酶活性。结果显示,肥胖大鼠TC、TAG、LDL、ALP、AST、ALT、MDA均显著高于对照组。另一方面,MP籽油通过改善TC、TAG和肝酶活性来改善hfd喂养大鼠的氧化应激和脂质代谢异常。这些结果表明,MP籽油可以有效地改善肥胖相关参数,减少肥胖相关并发症。
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引用次数: 0
Quantitative analysis of urban sprawl in Al-Baydah city - Libya, using GIS and remote sensing techniques. 利用GIS和遥感技术对利比亚Al-Baydah市城市扩张进行定量分析。
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.269848
Saleh Al saleh, Mohamed Abd El Galil, T. Hegazy, R. A. Abou Samra, MacTar Mohamed
Determining the spatio-temporal manners of urban sprawl is considered one of the most influential challenges in evaluating the present and future directions of the urban expansion issue. Random growth is considered as a kind of meeting the needs of the human being and expressing the achievement of its basic requirements when the state is unable to solve them. This paper aims to assess the sprawl and growth dynamics between 2010 and 2020 in Al-Baydah City, Libya using remote sensing and GIS techniques. Three Landsat TM, ETM+, and OLI images dated 2010, 2015, and 2020 were used to generate urban maps of the research area. The chi-square test and urban expansion intensity index (UEI) were used to assess urban growth patterns in the study zone. The results of the study confirmed that urban growth in the city has increased from 17.7 km2 in 2010 to 35.9 km2 in 2020, indicating that the city's expanding intensity index has risen as well. The results displayed that Al-Baydah city has unbalanced urban growth and its urban development had a high freedom degree during the period from 2010 to 2020.
确定城市扩张的时空方式被认为是评估城市扩张问题当前和未来方向的最具影响力的挑战之一。随机增长被认为是在国家无法解决人的基本需求的情况下,人的需求得到满足和满足的一种表现。本文旨在利用遥感和GIS技术评估2010年至2020年利比亚Al-Baydah市的扩张和增长动态。使用2010年、2015年和2020年的三幅Landsat TM、ETM+和OLI图像生成研究区的城市地图。采用卡方检验和城市扩张强度指数(UEI)对研究区城市增长模式进行评价。研究结果表明,上海市城市面积从2010年的17.7 km2增加到2020年的35.9 km2,城市扩张强度指数也有所上升。结果表明:2010 - 2020年,Al-Baydah市城市增长不平衡,城市发展自由度较高;
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引用次数: 0
Uropathogenic Bacterial Resistance isolated from Oncology Center in Mansoura University 曼苏拉大学肿瘤中心尿路病原菌耐药性分离株
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.269868
yahya bdreldien, M. Abou-Dobara, A. El-Sayed, Mohamed Zaghloul
Infections of Urinary tract are one of the significant to recommending antibiotic utilization. To involve the most perfect antibiotic for treatment patients, solid and ongoing information about the study of disease transmission and antibiotic resistance of pathogenic bacteria should be accessible for clinicians. So, the normal checking in every nation is required. The points of our review to explore the uropathogenic bacterial variety and antibiotics resistance rates of pathogenic bacteria at the Mansoura Oncology Center (Egypt, Mansoura city) from first October, 2019 to 30th September, 2020 and furthermore to add to checking and topographical variation of treatment by antibiotic. 150 urine samples by aretrospective analysis at Mansoura Oncology Center was processed according to the perfect protocol of urine analysis. Current results were prepared to obtain the prevalence of Urinary tract infections, antibiotics bacterial resistance, time over evolution, and multidrug resistance rate. Bacteria collected from 100 samples were the Escherichia coli and Klebsiella pneumoniae were to be the most identified with 42.0%, and 35.0% respectively. Other main detected species were Enterococci (9%), Pseudomonas (5%), Proteus mirabilis (4%), Citrobacter (2%), Breundimona (1%), Raoultella (1%), and Kluyvera cryocre-scens (1%). Resistance tests to antibiotics indicated K. pneumoniae was more resistance to Cefotriaxon 39.3%, while E. coli was resistant to Ciprofloxacin and Levofloxacin by 41.8%. The variety of some uropathogenic bacteria acquired had all the reserves of being resistant to various antibiotics. Ready faciing by arestorative impasse and the coming of bacteria with multi-resistant, recognizing explicit causes also is significant to adjust antibiotic treatment.
尿路感染是推荐使用抗生素的重要因素之一。为了让治疗患者使用最完美的抗生素,临床医生应该能够获得有关疾病传播和致病菌抗生素耐药性研究的可靠和持续的信息。因此,每个国家都需要进行正常的检查。目的探讨2019年10月1日至2020年9月30日埃及曼苏拉肿瘤中心尿路病原菌种类及病原菌耐药率,并对抗生素治疗的地形变化进行检查。曼苏拉肿瘤中心回顾性分析尿液样本150份,按照完善的尿液分析方案进行处理。目前的结果是准备获得尿路感染的患病率,抗生素细菌耐药性,进化时间和多药耐药率。其中大肠杆菌和肺炎克雷伯菌的检出率最高,分别为42.0%和35.0%。其他主要检出菌种为肠球菌(9%)、假单胞菌(5%)、奇异变形杆菌(4%)、柠檬酸杆菌(2%)、布隆地莫纳菌(1%)、拉乌尔氏菌(1%)和低温克卢韦拉菌(1%)。抗生素耐药试验结果显示,肺炎克雷伯菌对头孢曲松的耐药率为39.3%,大肠杆菌对环丙沙星和左氧氟沙星的耐药率为41.8%。所获得的部分尿路病原菌品种具有对各种抗生素耐药的全部储备。面对恢复性的僵局和多重耐药细菌的到来,认识明确的原因对调整抗生素治疗也具有重要意义。
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引用次数: 0
Magneto-Electric Influence on a Functionally Graded Porous Hollow Structure in Hygrothermal Environment 湿热环境下功能梯度多孔空心结构的磁电影响
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.170380.1062
Rania El-Tantawy
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引用次数: 0
Removal of Indigo dye from aqueous solution using amidoximated acrylonitrile /Na-Y- Zeolite composite 氨基肟化丙烯腈/Na-Y-沸石复合材料去除水溶液中的靛蓝染料
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.269857
M. Salem, Adel Elsonbaty, M. Diab, T. Al-Said
Adsorption of indigo dye onto amidoximated acrylonitrile /Na-Y-Zeolite composite had been evaluated in aqueous-solutions studying different parameters namely, period of the experiment, pH, adsorbent dosage and thermal effect on the experiment. Different properties of amidoximated acrylonitrile/sodium-Y-Zeolite composite as forier-transformation IR spectroscopy, X-rays deffraction had been investigated. Langmier and Frendlich sorption imitations had been utilized for describing equilibrial isotherm. In addition, evaluation of the activating force of sorption has been carried out regarding the sorption of indigo dye on the surface of amidoximated acrylonitrile/sodium-Y-Zeolite composite. Kinetics information of the reaction were also studied. These experimentally obtained information was nicely compatible and consistent with the pseudo-second-order kinetics for adsorption of indigo dye. The activating force (Ea), difference in liberated energies (∆G), entalpy (∆H) and enthropy (∆S) of sorption methodology had been determined regarding the sorption of the indigo dye via amidoximated acrylonitrile/sodium-Y-Zeolite composite. The thermodynamics of the sorption proved that the process had been both spontaneously and endothermically happening. The results indicated that amidoximated acrylonitrile/sodium-Y-Zeolite composite would be successfully applied as efficient, reproducible, and inexpensive adsorbent regarding the sorption of indigo dye from industrial wastewaters.
研究了偕胺肟化丙烯腈/ na - y -沸石复合材料对靛蓝染料的吸附性能,考察了实验时间、pH、吸附剂用量和热效应等参数对实验的影响。对偕胺肟化丙烯腈/ y型沸石钠复合材料的前变换红外光谱、x射线衍射等性能进行了研究。Langmier和frendich吸附模拟被用来描述平衡等温线。此外,还对靛蓝染料在偕胺肟化丙烯腈/ y型沸石钠复合材料表面的吸附活化剂进行了评价。研究了反应的动力学信息。这些实验所得的信息与靛蓝染料吸附的准二级动力学很好地相容和一致。测定了偕胺肟化丙烯腈/ y型沸石钠复合物对靛蓝染料的吸附方法的活化力(Ea)、释放能差(∆G)、焓(∆H)和熵(∆S)。吸附热力学证明了吸附过程是自发的,也是吸热的。结果表明,偕胺肟化丙烯腈/ y型沸石钠复合材料可作为高效、可再生、廉价的吸附剂用于工业废水中靛蓝染料的吸附。
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引用次数: 0
Pollen diversity of some woody plants in new Damietta city. 新达米埃塔市部分木本植物花粉多样性研究。
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.137961.1045
Ahmed Alnagger, S. Rabei, Aminah Abo Elnaga
The pollen morphology of 36 woody species from the New Damietta City, Egypt. Belonging to 20 families of angiosperms, distributed in eighteen dicots and two monocots were investigated. The pollen grains were acetolysed, measured, described and illustrated under light microscopy (LM). Examination of these families revealed great pollen diversity in their qualitative and quantitative characters. The results revealed 58.3% of species with prolate-spheroidal pollen, 16.7% with oblate-spheroidal pollen, 16.7% with subprolate pollen, 5.6% with prolate pollen and 2.7% with suboblate pollen. Tricolporate apertures accounted for almost half of apertures types; triporate, monocolpate, tricolpate, tetra- to penta-porate, tetraporate, pantoporate, inaperturate, tetracolporate and spiral aperture representing the remainder. Exine ornamentations ranged from psilate (33.3%), reticulate (27.7%) to micro-reticulate, scabrate, granulate, echinate each represents (2%) and clavate, verrucate, rugulate, striate, psilate-perforate, clavate-reticulate made up (1%) for each type. On the basis of pollen units, number and type of apertures, exine patterns were great diagnostic significance among the examined taxa. Six major pollen types and nine subtypes included in two groups (polyads and monads) were recognized. Description of pollen types, LM micrographs and a key for identification of the investigated taxa are provided.
埃及新达米埃塔市36种木本植物的花粉形态。属被子植物20科,分布于18个双子叶和2个单子叶。在光镜(LM)下对花粉粒进行了乙酰化、测量、描述和图解。结果表明,这些科的花粉在质和量性状上具有很大的多样性。结果表明:58.3%的植物花粉为长形球形,16.7%为扁球形,16.7%为近长形,5.6%为长形,2.7%为近短形。三孔孔径几乎占所有孔径类型的一半;三孔、单孔、三孔、四孔到五孔、四孔、四孔、无孔、四孔和螺旋孔代表其余部分。每一种类型的外壁纹饰由柱状(33.3%)、网状(27.7%)到微网状、锯齿状、颗粒状、棘刺状各占(2%),以及棒状、疣状、规则状、条纹状、柱状-穿孔、棒状-网状各占(1%)。根据花粉单位、花粉孔数和花粉孔类型,外壁模式在各分类群中具有重要的诊断意义。鉴定出6种主要的花粉类型和9个亚型,包括两组(多倍体和单倍体)。提供了花粉类型的描述,LM显微照片和鉴定分类群的关键。
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引用次数: 0
Vegetation and Biochemical contents of Caulerpa racemosa from Red Sea Coast at Hurghada-Egypt 埃及赫尔格达红海沿岸总状蕨的植被和生化成分
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.269831
S. A. Abu Ahmed, M. Deyab, Randa Ahmed Ali, A. Hyder
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引用次数: 0
Production and Optimization of Gelatinase Producing Lentzea sp. Strain Isolated from the Soil Rhizosphere 产明胶酶Lentzea sp.土壤根际分离菌株的生产与优化
Pub Date : 2022-12-01 DOI: 10.21608/sjdfs.2022.269835
Yara Batroukha, M. M. Nour el Dein, M. Abou-Dobara, A. El‐Sayed
Proteases are the major enzymes that have been used widely in health, food, and nutritional supplement fields. Rare actinobacteria are considered a new resource for proteolytic enzyme activity. Rare actinobacteria are used to be isolated from marine and have been studied under restricted limits. The strain used in this study was identified as Lentzea sp. which was successfully isolated from the soil at the Faculty of Science, Damietta, Egypt. It was subjected to gelatin liquefaction as primary screening for gelatinolytic activity. Based on this study, the best gelatinolytic (gelatinase) activity was noticed within 8 days at 37oC (17.59 U/ml ±0.06). The maximum activity was obtained at pH 9 (28.33 U/ml ±0.04) and agitation speed of 150 rpm (29.10 U/ml ±0.04). Upon carbon and nitrogen source optimization, the basal medium of combination between gelatin and peptone produced maximal gelatinase activity (29.12 U/ml ±0.06) and it was found that 10% sucrose was the best carbon source that produced maximum gelatinase activity at (30.17 U/ml ±0.05).
蛋白酶是广泛应用于保健、食品和营养补充领域的主要酶。稀有放线菌被认为是蛋白质水解酶活性的新资源。稀有放线菌是从海洋中分离出来的,并在有限的条件下进行了研究。本研究中使用的菌株被鉴定为Lentzea sp.,它成功地从埃及达米埃塔科学学院的土壤中分离出来。对其进行明胶液化作为明胶水解活性的初步筛选。在37℃(17.59 U/ml±0.06)条件下,8 d内明胶酶活性最高。在pH为9 (28.33 U/ml±0.04)、搅拌转速为150 rpm (29.10 U/ml±0.04)时活性最高。通过碳氮源优化,明胶与蛋白胨组合的基础培养基明胶酶活性最高(29.12 U/ml±0.06),10%蔗糖为最佳碳源,明胶酶活性最高(30.17 U/ml±0.05)。
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引用次数: 0
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Scientific Journal for Damietta Faculty of Science
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