V. Kozhyn, V. Salata, M. Kukhtyn, Y. Horiuk, T. S. Matviishyn
The main task of carrying out disinfection measures in veterinary medicine is to break the mechanisms of pathogen transmission through objects and objects of the environment from one animal to another. Therefore, disinfection measures are the basis of safe keeping of animals, carrying out treatment and preventive measures in clinics of veterinary medicine. The purpose of this study was to determine the effectiveness of the created disinfectant “Enzidez” in the production conditions of veterinary medicine clinics. The surfaces of walls, floors and tables were disinfected by wiping. Surgical, dental instruments and veterinary products by immersion in a solution or wiping. Before and after treatment, washings were taken for microbiological examination by generally accepted methods. It was established that the effectiveness of disinfection with “Enzidez” in a concentration of 0.25–1.00 % of walls, floors, tables in veterinary clinics, for their various microbial and organic contamination, was 100 %. Only in one case, after disinfection with a 0.25 % solution, microorganisms were released from the floor surfaces, amounting to 3.8 ± 0.1×101 CFU/cm3 of wash. However, even under this regime, the disinfection efficiency was 99.99 %. The effectiveness of disinfection with “Enzidez” during the presterilization cleaning of surgical instruments and veterinary products revealed that the main microflora before treatment was represented by staphylococci, micrococci, corynebacteria, streptococci, yeast, coliform bacteria in the amount of 103 to 105 CFU/cm3 of washing. After treatment with a concentration of 0.25 to 1.00 %, the disinfectant provided a bactericidal effect, as a result of which microorganisms were not isolated from the surfaces in any case. Therefore, we believe that the developed means for disinfection, pre-sterilization cleaning and sterilization in veterinary medicine clinics “Enzidez” is a highly effective disinfectant for use in a concentration of 0.25–1.0 % and exposure of solutions for 15–30 minutes.
{"title":"Production studies of the disinfectant “Enzidez”","authors":"V. Kozhyn, V. Salata, M. Kukhtyn, Y. Horiuk, T. S. Matviishyn","doi":"10.32718/nvlvet11112","DOIUrl":"https://doi.org/10.32718/nvlvet11112","url":null,"abstract":"The main task of carrying out disinfection measures in veterinary medicine is to break the mechanisms of pathogen transmission through objects and objects of the environment from one animal to another. Therefore, disinfection measures are the basis of safe keeping of animals, carrying out treatment and preventive measures in clinics of veterinary medicine. The purpose of this study was to determine the effectiveness of the created disinfectant “Enzidez” in the production conditions of veterinary medicine clinics. The surfaces of walls, floors and tables were disinfected by wiping. Surgical, dental instruments and veterinary products by immersion in a solution or wiping. Before and after treatment, washings were taken for microbiological examination by generally accepted methods. It was established that the effectiveness of disinfection with “Enzidez” in a concentration of 0.25–1.00 % of walls, floors, tables in veterinary clinics, for their various microbial and organic contamination, was 100 %. Only in one case, after disinfection with a 0.25 % solution, microorganisms were released from the floor surfaces, amounting to 3.8 ± 0.1×101 CFU/cm3 of wash. However, even under this regime, the disinfection efficiency was 99.99 %. The effectiveness of disinfection with “Enzidez” during the presterilization cleaning of surgical instruments and veterinary products revealed that the main microflora before treatment was represented by staphylococci, micrococci, corynebacteria, streptococci, yeast, coliform bacteria in the amount of 103 to 105 CFU/cm3 of washing. After treatment with a concentration of 0.25 to 1.00 %, the disinfectant provided a bactericidal effect, as a result of which microorganisms were not isolated from the surfaces in any case. Therefore, we believe that the developed means for disinfection, pre-sterilization cleaning and sterilization in veterinary medicine clinics “Enzidez” is a highly effective disinfectant for use in a concentration of 0.25–1.0 % and exposure of solutions for 15–30 minutes.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"2013 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. V. Kladnytska, V. S. Velychko, V. A. Tomchuk, V. Z. Salata, S. V. Velychko, N. V. Dyshlyuk, T. A. Mazurkevich, S. V. Midyk, R. R. Bokotko, T. L. Savchuk
The content of fatty acids in the lipids of mesenchymal stem cells of dog adipose tissue culture was studied. Mesenchymal stem cells of dog adipose tissue culture were obtained by culturing the primary material in a CO2 incubator with a content of 5 % CO2, at a temperature of 37 °C in DMEM medium with the addition of 10–15 % fetal bovine serum and 1 % antibiotic-antimycotic. When the confluency of the monolayer reached 70–80 %, the cells were transferred to a suspension and subcultivated in order to reduce the heterogeneity of the culture and obtain a sufficient amount of biological material. The lipids of the obtained stem cells were analyzed for the content of fatty acids by the method of thin-layer gas-liquid chromatography. Determination of the content of lipids of fatty acids in FSK of a cat was carried out by the Folch method. A mixture of fatty acid methyl esters was analyzed on a Trace GC Ultra gas chromatograph with a flame ionization detector on a capillary column SPTM –2560, 100 m x 0.25 mm ID, 0.20 μm film (Supelco). Identification of fatty acids was carried out using a standard sample of Supelco 37 Сomponent FAME Mix. Quantitative assessment of the LC spectrum was carried out by the method of normalization of the peak planes of methylated LC derivatives and their content was determined as a percentage of the total content of all LC. The conducted study of the content of fatty acids in lipids made it possible to reveal certain features of the lipid metabolism of mesenchymal stem cells cultured in dog adipose tissue. A high content of oleic acid, characteristic of cells resistant to apoptosis and with high proliferative potential, was determined; a high ratio of unsaturated linoleic to saturated stearic acid (С18:1/С18.0), which reflects the high activity of the stearoyl-coenzyme-desaturase enzyme and, indirectly, the active state of the Wnt/β-catenin signaling pathway; inability to lengthen the chain of saturated fatty acids; lack or low activity of de novo synthesis of omega-6 polyunsaturated fatty acids. 18 fatty acids were found in the composition of lipids of fetal stem cells of a cat, of the saturated ones - the most palmitic acid (33.70 ± 0.02 %), of the monounsaturated ones – oleic acid (21.63 ± 0.03 %), of the polyunsaturated ones – linoleic acid (6.45 ± 0.07 %). The least amount of cis-,11,14-eicosadienoic acid (0.04 ± 0.01 %) was found in the composition of cell lipids. The total amount of saturated fatty acids in dog mesenchymal stem cell lipids was 65.65 ± 0.02 %), unsaturated fatty acids – 34.35 ± 0.02 %. Monoene fatty acids were determined in the amount of 24.46 ± 0.02 %, and polyene – 9.89 ± 0.02 %. The ratio index of polyunsaturated fatty acids ω 3 to ω 6 is 0.40. Lipids of mesenchymal stem cells of adipose tissue culture were characterized by a lower content of monoene unsaturated fatty acids 24.46 ± 0.02; (P < 0.05), with a higher content of ω3 fatty acids 3.04 ± 0.02 %; (P < 0.05), with a lower content of ω6 fatty a
{"title":"Content of fatty acids in lipids of adipose derived mesenchymal stem cells","authors":"L. V. Kladnytska, V. S. Velychko, V. A. Tomchuk, V. Z. Salata, S. V. Velychko, N. V. Dyshlyuk, T. A. Mazurkevich, S. V. Midyk, R. R. Bokotko, T. L. Savchuk","doi":"10.32718/nvlvet11120","DOIUrl":"https://doi.org/10.32718/nvlvet11120","url":null,"abstract":"The content of fatty acids in the lipids of mesenchymal stem cells of dog adipose tissue culture was studied. Mesenchymal stem cells of dog adipose tissue culture were obtained by culturing the primary material in a CO2 incubator with a content of 5 % CO2, at a temperature of 37 °C in DMEM medium with the addition of 10–15 % fetal bovine serum and 1 % antibiotic-antimycotic. When the confluency of the monolayer reached 70–80 %, the cells were transferred to a suspension and subcultivated in order to reduce the heterogeneity of the culture and obtain a sufficient amount of biological material. The lipids of the obtained stem cells were analyzed for the content of fatty acids by the method of thin-layer gas-liquid chromatography. Determination of the content of lipids of fatty acids in FSK of a cat was carried out by the Folch method. A mixture of fatty acid methyl esters was analyzed on a Trace GC Ultra gas chromatograph with a flame ionization detector on a capillary column SPTM –2560, 100 m x 0.25 mm ID, 0.20 μm film (Supelco). Identification of fatty acids was carried out using a standard sample of Supelco 37 Сomponent FAME Mix. Quantitative assessment of the LC spectrum was carried out by the method of normalization of the peak planes of methylated LC derivatives and their content was determined as a percentage of the total content of all LC. The conducted study of the content of fatty acids in lipids made it possible to reveal certain features of the lipid metabolism of mesenchymal stem cells cultured in dog adipose tissue. A high content of oleic acid, characteristic of cells resistant to apoptosis and with high proliferative potential, was determined; a high ratio of unsaturated linoleic to saturated stearic acid (С18:1/С18.0), which reflects the high activity of the stearoyl-coenzyme-desaturase enzyme and, indirectly, the active state of the Wnt/β-catenin signaling pathway; inability to lengthen the chain of saturated fatty acids; lack or low activity of de novo synthesis of omega-6 polyunsaturated fatty acids. 18 fatty acids were found in the composition of lipids of fetal stem cells of a cat, of the saturated ones - the most palmitic acid (33.70 ± 0.02 %), of the monounsaturated ones – oleic acid (21.63 ± 0.03 %), of the polyunsaturated ones – linoleic acid (6.45 ± 0.07 %). The least amount of cis-,11,14-eicosadienoic acid (0.04 ± 0.01 %) was found in the composition of cell lipids. The total amount of saturated fatty acids in dog mesenchymal stem cell lipids was 65.65 ± 0.02 %), unsaturated fatty acids – 34.35 ± 0.02 %. Monoene fatty acids were determined in the amount of 24.46 ± 0.02 %, and polyene – 9.89 ± 0.02 %. The ratio index of polyunsaturated fatty acids ω 3 to ω 6 is 0.40. Lipids of mesenchymal stem cells of adipose tissue culture were characterized by a lower content of monoene unsaturated fatty acids 24.46 ± 0.02; (P < 0.05), with a higher content of ω3 fatty acids 3.04 ± 0.02 %; (P < 0.05), with a lower content of ω6 fatty a","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To increase the sexual activity and sperm quality of rams during the period of sexual rest, it is necessary to increase the consumption rates of vitamins and microelements to the level of the mating season. The aim of the work was to find out the effect of feeding a liposomal vitamin-mineral supplement on the quality parameters of ram sperm during the period of sexual rest. The experiment was conducted on 12 clinically healthy Texel rams, aged 2–4 years, during the period of sexual rest (March–May). Animals were divided into two groups: control and experimental, 6 heads each. The control rams received the basic diet, the animals of the experimental group were individually added to the combined feed for 45 days with a feed additive in the form of a liposomal emulsion, which included: vitamins A, D3, E, C and zinc gluconate. After the end of supplement feeding, ejaculates were collected from the rams for research. The motility, kinetic indicators of sperm, the activity of succinate dehydrogenase (SDH) and cytochrome oxidase (CO) in sperm, as well as the activity of antioxidant protection enzymes superoxide dismutase (SOD), glutathione peroxidase (GPO) and catalase (CAT) were determined. It was established that feeding liposomal vitamin-mineral supplement to rams during the period of sexual rest significantly (P < 0.05) increases sperm activity, their kinetic indicators: curvilinear (VCL), straight-line (VSL) and average speed (VAP) of sperm movement (P < 0.05–0.001), the activity of enzymes – markers of the fertilizing capacity of SDH and CO sperm (P < 0.05–0.001). At the same time, there was a significant (P < 0.05) decrease in the activity of SOD with a simultaneous significant (by 23.3–25.0 %) increase in the activity of GPO and CAT (P < 0.01). Higher indicators of the quality of ejaculates of rams under the influence of vitamins A, D3, E, C and zinc gluconate indicate the possibility of obtaining high-quality sperm from breeding rams during the period of sexual rest.
{"title":"Semen quality of rams fed a liposomal vitamin-mineral supplement during the period of sexual rest","authors":"O. Sharan","doi":"10.32718/nvlvet11113","DOIUrl":"https://doi.org/10.32718/nvlvet11113","url":null,"abstract":"To increase the sexual activity and sperm quality of rams during the period of sexual rest, it is necessary to increase the consumption rates of vitamins and microelements to the level of the mating season. The aim of the work was to find out the effect of feeding a liposomal vitamin-mineral supplement on the quality parameters of ram sperm during the period of sexual rest. The experiment was conducted on 12 clinically healthy Texel rams, aged 2–4 years, during the period of sexual rest (March–May). Animals were divided into two groups: control and experimental, 6 heads each. The control rams received the basic diet, the animals of the experimental group were individually added to the combined feed for 45 days with a feed additive in the form of a liposomal emulsion, which included: vitamins A, D3, E, C and zinc gluconate. After the end of supplement feeding, ejaculates were collected from the rams for research. The motility, kinetic indicators of sperm, the activity of succinate dehydrogenase (SDH) and cytochrome oxidase (CO) in sperm, as well as the activity of antioxidant protection enzymes superoxide dismutase (SOD), glutathione peroxidase (GPO) and catalase (CAT) were determined. It was established that feeding liposomal vitamin-mineral supplement to rams during the period of sexual rest significantly (P < 0.05) increases sperm activity, their kinetic indicators: curvilinear (VCL), straight-line (VSL) and average speed (VAP) of sperm movement (P < 0.05–0.001), the activity of enzymes – markers of the fertilizing capacity of SDH and CO sperm (P < 0.05–0.001). At the same time, there was a significant (P < 0.05) decrease in the activity of SOD with a simultaneous significant (by 23.3–25.0 %) increase in the activity of GPO and CAT (P < 0.01). Higher indicators of the quality of ejaculates of rams under the influence of vitamins A, D3, E, C and zinc gluconate indicate the possibility of obtaining high-quality sperm from breeding rams during the period of sexual rest.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nowadays, the importance of medicinal plants has increased significantly as a source of biologically active substances, including antibacterial action. There is an intensive search for effective and safe drugs that could perform the function of preventive disinfection, reduce the negative impact on the animal's body and the environment as a whole, and be economically beneficial. Therefore, we chose 17 promising types of plant raw materials for the study due to the sufficient prevalence and diversity of chemical composition. The scientific work presents the results of the effectiveness of ethanolic plant extracts against aureus, Echerihia coli and Staphylococcus. The antibacterial effect of plant tinctures of Kalgan, Comfrey, Bearberry, Geranium, Nut membranes and Dandelion on St. Aureus strain 209 P and E. coli strain 1257 was revealed. According to the results of the studies, high and moderate antibacterial activity of plant materials was found, which is an effective means of preventive measures in veterinary medicine. It has also been found that the “wells” method is more effective than the paper disc method and gives a more accurate result, so it is relevant in further research. Herbal medicines account for more than 50 % of all pharmaceuticals. A significant place among them is occupied by prophylactic disinfectants used to prevent diseases. The main active substances of such products are secondary metabolites of medicinal plants, which include phenols and flavonoids, that in turn have antimicrobial effects. The content of these substances is controlled by analyzing extracts made from plant material. Calgan is a perennial medicinal plant of the Pink family that has a number of powerful properties. The rhizome of this plant contains flavonoids, wax, iron, starch, malic acid, essential oils, catechin, glycosides, vitamins PP, A, Z. Comfrey is a perennial herbaceous plant that belongs to the family of broadleaves. The highest concentration of nutrients is found in the rhizome of the three-year-old plant: tannins, amino acids, alkaloids, flavonoids, etc. Geranium is a perennial herb of the geranium family, rich in tannins – tannins are found in the rhizome and ground part of the plant. It also contains flavonoids, triterpene saponins, carotene, vitamin C, and starch. Dandelion is a perennial plant from the aster family. The roots contain inulin, fructose, organic acids, steroids, flavonoids, tannins, waxes, and higher fatty acids. The walnut membranes are rich in iodine, zinc, magnesium, ascorbic and nicotinic acids, PP and B vitamins, tannins, and essential oils. Bearberry is a genus of evergreen hardwood shrubs of the heather family. The main active ingredients are phenolic compounds.
{"title":"Antimicrobial properties of ethanolic plant extracts on microorganisms of the genus Staphylococcus aureus, Echerihia coli","authors":"A. M. Khyl, S. B. Peredera","doi":"10.32718/nvlvet11116","DOIUrl":"https://doi.org/10.32718/nvlvet11116","url":null,"abstract":"Nowadays, the importance of medicinal plants has increased significantly as a source of biologically active substances, including antibacterial action. There is an intensive search for effective and safe drugs that could perform the function of preventive disinfection, reduce the negative impact on the animal's body and the environment as a whole, and be economically beneficial. Therefore, we chose 17 promising types of plant raw materials for the study due to the sufficient prevalence and diversity of chemical composition. The scientific work presents the results of the effectiveness of ethanolic plant extracts against aureus, Echerihia coli and Staphylococcus. The antibacterial effect of plant tinctures of Kalgan, Comfrey, Bearberry, Geranium, Nut membranes and Dandelion on St. Aureus strain 209 P and E. coli strain 1257 was revealed. According to the results of the studies, high and moderate antibacterial activity of plant materials was found, which is an effective means of preventive measures in veterinary medicine. It has also been found that the “wells” method is more effective than the paper disc method and gives a more accurate result, so it is relevant in further research. Herbal medicines account for more than 50 % of all pharmaceuticals. A significant place among them is occupied by prophylactic disinfectants used to prevent diseases. The main active substances of such products are secondary metabolites of medicinal plants, which include phenols and flavonoids, that in turn have antimicrobial effects. The content of these substances is controlled by analyzing extracts made from plant material. Calgan is a perennial medicinal plant of the Pink family that has a number of powerful properties. The rhizome of this plant contains flavonoids, wax, iron, starch, malic acid, essential oils, catechin, glycosides, vitamins PP, A, Z. Comfrey is a perennial herbaceous plant that belongs to the family of broadleaves. The highest concentration of nutrients is found in the rhizome of the three-year-old plant: tannins, amino acids, alkaloids, flavonoids, etc. Geranium is a perennial herb of the geranium family, rich in tannins – tannins are found in the rhizome and ground part of the plant. It also contains flavonoids, triterpene saponins, carotene, vitamin C, and starch. Dandelion is a perennial plant from the aster family. The roots contain inulin, fructose, organic acids, steroids, flavonoids, tannins, waxes, and higher fatty acids. The walnut membranes are rich in iodine, zinc, magnesium, ascorbic and nicotinic acids, PP and B vitamins, tannins, and essential oils. Bearberry is a genus of evergreen hardwood shrubs of the heather family. The main active ingredients are phenolic compounds.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Against the background of reduced immunity of the body, the role of pathogenic and opportunistic microorganisms, which usually circulate in poultry farms in various associations, among the causative agents of poultry bacteriosis, is sharply increasing. Salmonella is one of the main bacteria affecting poultry and the most essential zoonotic pathogen causing foodborne illness. The work aimed to search and isolate lytic phages active in museum strains of Salmonella from the environment of poultry farms. The presence of lytic phages in museum strains of salmonella (Salmonella typhimurium 144 and Salmonella adobraco 1) was determined in the poultry houses samples according to Oliver and Grazia's standard method. It was established that the frequency of isolation of bacteriophages to the strain Salmonella typhimurium 144 from poultry farms was from 17.6 to 30.8 %, and the strain Salmonella adobraco 1 was 1.5 to 3.0 times less. In contrast, most often, lytic bacteriophages were isolated from sewage poultry houses. The size of negative colonies was larger in bacteriophages against Salmonella typhimurium 144 strain and was 2.8 ± 0.3 mm, which is 1.4 times larger, compared with colonies of bacteriophages lytic against Salmonella adobraco 1 strain. According to the degree of transparency, bacteriophages of Salmonella typhimurium 144 strain were primarily transparent. At the same time, the colonies of Salmonella adobraco 1 phages were translucent in most cases. Therefore, isolated bacteriophages for the Salmonella typhimurium 144 strain are better suited for developing a biological preparation for industrial poultry farming.
{"title":"Isolation and characterization of bacteriophages Salmonella spp.","authors":"O. Vasylkiv, M. Kukhtyn","doi":"10.32718/nvlvet11108","DOIUrl":"https://doi.org/10.32718/nvlvet11108","url":null,"abstract":"Against the background of reduced immunity of the body, the role of pathogenic and opportunistic microorganisms, which usually circulate in poultry farms in various associations, among the causative agents of poultry bacteriosis, is sharply increasing. Salmonella is one of the main bacteria affecting poultry and the most essential zoonotic pathogen causing foodborne illness. The work aimed to search and isolate lytic phages active in museum strains of Salmonella from the environment of poultry farms. The presence of lytic phages in museum strains of salmonella (Salmonella typhimurium 144 and Salmonella adobraco 1) was determined in the poultry houses samples according to Oliver and Grazia's standard method. It was established that the frequency of isolation of bacteriophages to the strain Salmonella typhimurium 144 from poultry farms was from 17.6 to 30.8 %, and the strain Salmonella adobraco 1 was 1.5 to 3.0 times less. In contrast, most often, lytic bacteriophages were isolated from sewage poultry houses. The size of negative colonies was larger in bacteriophages against Salmonella typhimurium 144 strain and was 2.8 ± 0.3 mm, which is 1.4 times larger, compared with colonies of bacteriophages lytic against Salmonella adobraco 1 strain. According to the degree of transparency, bacteriophages of Salmonella typhimurium 144 strain were primarily transparent. At the same time, the colonies of Salmonella adobraco 1 phages were translucent in most cases. Therefore, isolated bacteriophages for the Salmonella typhimurium 144 strain are better suited for developing a biological preparation for industrial poultry farming.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cystoisosporosis refers to a protozoan parasitic disease caused by coccidia of the genus Cystoisospora canis in dogs, especially young ones. Pathogens are localized in the small intestine of animals and lead to an imbalance of microflora, indigestion, enteritis, and diarrhea. Knowledge of the epizootological features of this invasion makes it possible to carry out complex preventive measures more effectively and maintain well-being in certain territories. The research aimed to establish the dependence of the infection of dogs with the causative agent of cystoisosporosis on their age and breed. The conducted studies established that the age-related dynamics of cystoisosoporosis in the conditions of the city of Kharkiv were characterized by a decrease in the extent of infestation with the age of the dogs. The most affected were young animals up to 6 months of age, where the extent of infestation was 32.0 %. In dogs aged 6–12 months and 1–3 years, the extent of cystoisosporous infestation decreases to 18.9 and 4.7 %, respectively. Dogs aged 3 to 6 years and older than six were the least affected by cystoisospor oocysts, where the extent of infestation is 4.7 and 2.7 %, respectively. Breed susceptibility to cystoisosprosis is characterized by the most excellent infestation of mixed-breed dogs and purebred animals, where their average infestation was at the level of 22.0 %. Cystoisosporosis was less often diagnosed in dogs of hunting, service, and decorative breeds; the extent of infestation ranged from 10.3 to 14.3 %. In terms of breeds, the highest infestation by protozoan organisms was found in labrador retrievers (extensive infestation 6.0 %), German shepherds (3.9 %), dachshunds (3.5 %), Yorkshire terriers (3.2 %) and cocker spaniels (2.8 %). The obtained data allow us to take into account the obtained results regarding the age and breed susceptibility of dogs to coccidia of the genus Cystoisospora for timely diagnosis and prevention of cystoisosporosis.
{"title":"Age and breed susceptibility of dogs to the causative agent of cystoisosporosis","authors":"R. Suvorov, V. Melnychuk","doi":"10.32718/nvlvet11111","DOIUrl":"https://doi.org/10.32718/nvlvet11111","url":null,"abstract":"Cystoisosporosis refers to a protozoan parasitic disease caused by coccidia of the genus Cystoisospora canis in dogs, especially young ones. Pathogens are localized in the small intestine of animals and lead to an imbalance of microflora, indigestion, enteritis, and diarrhea. Knowledge of the epizootological features of this invasion makes it possible to carry out complex preventive measures more effectively and maintain well-being in certain territories. The research aimed to establish the dependence of the infection of dogs with the causative agent of cystoisosporosis on their age and breed. The conducted studies established that the age-related dynamics of cystoisosoporosis in the conditions of the city of Kharkiv were characterized by a decrease in the extent of infestation with the age of the dogs. The most affected were young animals up to 6 months of age, where the extent of infestation was 32.0 %. In dogs aged 6–12 months and 1–3 years, the extent of cystoisosporous infestation decreases to 18.9 and 4.7 %, respectively. Dogs aged 3 to 6 years and older than six were the least affected by cystoisospor oocysts, where the extent of infestation is 4.7 and 2.7 %, respectively. Breed susceptibility to cystoisosprosis is characterized by the most excellent infestation of mixed-breed dogs and purebred animals, where their average infestation was at the level of 22.0 %. Cystoisosporosis was less often diagnosed in dogs of hunting, service, and decorative breeds; the extent of infestation ranged from 10.3 to 14.3 %. In terms of breeds, the highest infestation by protozoan organisms was found in labrador retrievers (extensive infestation 6.0 %), German shepherds (3.9 %), dachshunds (3.5 %), Yorkshire terriers (3.2 %) and cocker spaniels (2.8 %). The obtained data allow us to take into account the obtained results regarding the age and breed susceptibility of dogs to coccidia of the genus Cystoisospora for timely diagnosis and prevention of cystoisosporosis.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This review presents data on the most common causative agents of protozooses, helminthiases, and ectoparasitic diseases of rabbits (Oryctolagus cuniculus, Linnaeus, 1758), which are of interest to veterinarians. Modern methods of diagnosing parasitic diseases in rabbits have been determined. Chemotherapeutic drugs available on the world pharmaceutical market for therapeutic and preventive treatments in rabbits are listed. The main causative agents of protozoan, helminthic, and ectoparasitic diseases of rabbits are considered, considering the modern systematic position of parasites. The features of the distribution of parasitic diseases in Ukraine and the world, according to the data of well-known scientists in the field of veterinary parasitology, are briefly given. Information on the specifics of the use of antiparasitic drugs in the case of animals affected by protozooses, helminths, and ticks is summarized. The work briefly provides information on the most common medicines and chemical combinations that are the active ingredients of these drugs (their chemical names existing in different countries, synonyms, and main pharmacological properties). Information on the dosage and features of the use of antiparasitic agents in the case of rabbit diseases is given following the data of modern scientific literature and the relevant recommendations for their industrial use. During the inspection, it was established that rabbits are most often affected by eimeriosis (coccidiosis). Currently, 15 species of Eimeria spp. have been identified. Young animals (2–6 months) are most susceptible to eimeriosis. The peak of infestation in the territory of Ukraine falls in the spring-summer period. It was found that P. ambiguus is most often detected among helminthiases in rabbits in our country. Rabbits aged 1 to 2 years are most susceptible to these helminths, and the peak of infestation occurs in January. In rabbits, psoroptosis is one of the most common acaroses, the causative agent of which is ticks of the P. cuniculi species. The purpose of this work was to show the current state of rabbits' most common parasitic diseases on the territory of Ukraine and in the world in general, highlight the existing antiparasitic drugs, and summarize information on their use. It has been established that animals with encephalitozoonosis are the most difficult to diagnose and treat. At the same time, there is a wide range of coccidiostats and coccidiolytics for treating rabbits affected by eimeries. For the infestation of animals with helminths, it is appropriate to use benzimidazoles and ivermectins. In the case of acaroses, ivermectin and fungicidal-acaricidal ointments remain effective drugs. The analysis of literary sources will allow for the expansion of the already available data on the spread, prevention, and treatment of parasitic diseases in rabbits. The given information will help ensure the rabbits' veterinary well-being on Ukraine's territory.
{"title":"Parasitic diseases of rabbits (distribution, diagnosis and treatment)","authors":"O. V. Kruchynenko","doi":"10.32718/nvlvet11109","DOIUrl":"https://doi.org/10.32718/nvlvet11109","url":null,"abstract":"This review presents data on the most common causative agents of protozooses, helminthiases, and ectoparasitic diseases of rabbits (Oryctolagus cuniculus, Linnaeus, 1758), which are of interest to veterinarians. Modern methods of diagnosing parasitic diseases in rabbits have been determined. Chemotherapeutic drugs available on the world pharmaceutical market for therapeutic and preventive treatments in rabbits are listed. The main causative agents of protozoan, helminthic, and ectoparasitic diseases of rabbits are considered, considering the modern systematic position of parasites. The features of the distribution of parasitic diseases in Ukraine and the world, according to the data of well-known scientists in the field of veterinary parasitology, are briefly given. Information on the specifics of the use of antiparasitic drugs in the case of animals affected by protozooses, helminths, and ticks is summarized. The work briefly provides information on the most common medicines and chemical combinations that are the active ingredients of these drugs (their chemical names existing in different countries, synonyms, and main pharmacological properties). Information on the dosage and features of the use of antiparasitic agents in the case of rabbit diseases is given following the data of modern scientific literature and the relevant recommendations for their industrial use. During the inspection, it was established that rabbits are most often affected by eimeriosis (coccidiosis). Currently, 15 species of Eimeria spp. have been identified. Young animals (2–6 months) are most susceptible to eimeriosis. The peak of infestation in the territory of Ukraine falls in the spring-summer period. It was found that P. ambiguus is most often detected among helminthiases in rabbits in our country. Rabbits aged 1 to 2 years are most susceptible to these helminths, and the peak of infestation occurs in January. In rabbits, psoroptosis is one of the most common acaroses, the causative agent of which is ticks of the P. cuniculi species. The purpose of this work was to show the current state of rabbits' most common parasitic diseases on the territory of Ukraine and in the world in general, highlight the existing antiparasitic drugs, and summarize information on their use. It has been established that animals with encephalitozoonosis are the most difficult to diagnose and treat. At the same time, there is a wide range of coccidiostats and coccidiolytics for treating rabbits affected by eimeries. For the infestation of animals with helminths, it is appropriate to use benzimidazoles and ivermectins. In the case of acaroses, ivermectin and fungicidal-acaricidal ointments remain effective drugs. The analysis of literary sources will allow for the expansion of the already available data on the spread, prevention, and treatment of parasitic diseases in rabbits. The given information will help ensure the rabbits' veterinary well-being on Ukraine's territory.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. I. Kovalchuk, R. L. Androshulik, A. Z. Pylypets, M. M. Tsap
Mineral elements increase the capacity of organisms for their adaptation to abiotic factors and improve the biological value of animal products. The aim of the experiment was to study the effect of magnesium citrate on the content of total protein in the tissues of the whole organism, the content of soluble protein fractions in hemolymph, and catalase activity. The research was conducted on Carpathian honey bees. They were selected in the apiary at the Institute of Animal Biology of the National Academy of Sciences. The research was conducted in two stages. The first stage of the work was carried out on 5 groups of bees under the conditions of a laboratory thermostat for 20 days. Bees of the control group were fed daily with 1 ml of 50 % sugar syrup (SS) and 1 ml of H2O; group II – 1 ml of SS + 1 ml of 0.4 mg Mg/l nanocitrate; group III – 1 ml of CS + 1 ml of 2 mg Mg/l citrate; group IV – 1 ml of SS + 1 ml of 3 mg Mg/l citrate; group V – 1 ml of SS + 1 ml of 4 mg Mg/l citrate. The second stage of the study was conducted on four groups of bees and lasted 30 days. Bees of the control (I) group were fed daily with 1 ml of 50 % SS and 1 ml of H2O; group II – 1 ml of SS + 1 ml of 0.04 mg Mg/l citrate; group III – 1 ml of SS + 1 ml of 0.02 mg Mg/l citrate; group IV – 1 ml of SS + 1 ml of 0.01 mg Mg/l citrate. At the first stage, a decrease in α1-globulins in the hemolymph of bees of the III – V groups was established. The content of β-globulins increased in the II (P < 0.001), III (P < 0.01), IV (P < 0.001) and V (P < 0.001) experimental groups. The content of γ-globulins decreased in hemolymph of II (P < 0.001) and III (P < 0.01) groups. At the second stage, a decrease in α1–globulins was observed in the hemolymph of bees of the II, III and IV experimental groups compared to the control. The content of α2-globulins was significantly lower in the hemolymph of bees from IV group, and the content of β-globulins was higher in the hemolymph of the bees. An increase in the content of γ-globulins was established in II (P < 0.05), III and IV (P < 0.01) experimental groups. High catalase activity was observed in the hemolymph of bees of all experimental groups (P < 0.001). The highest catalase activity was registered in bees of IV group. The use of 0.01 mg of Mg citrate in addition to sugar syrup feeding changed the ratio of individual hemolymph protein fractions. The relative content of albumin and β-globulin decreased and α2 and γ globulin content increased. An increase in the relative content of α1 and a decrease in β- and γ-globulins in the hemolymph of honey bees of the research groups were caused by Mg citrate in a dose of 0.04 mg.
矿物元素增加了生物体对非生物因素的适应能力，提高了动物产品的生物学价值。本试验旨在研究柠檬酸镁对全生物组织中总蛋白含量、血淋巴中可溶性蛋白组分含量及过氧化氢酶活性的影响。这项研究是在喀尔巴阡蜜蜂身上进行的。它们是在美国国家科学院动物生物学研究所的养蜂场中挑选的。研究分两个阶段进行。第一阶段的工作是在实验室恒温器条件下对5组蜜蜂进行20天的研究。对照组蜜蜂每天喂食1 ml 50%糖浆(SS)和1 ml H2O;II组- SS 1ml +纳米柠檬酸盐0.4 mg /l 1ml;III组:CS 1ml +枸橼酸2mg /l 1ml;IV组- SS 1ml + 3mg mg /l柠檬酸盐1ml;V组- SS 1ml +柠檬酸盐4mg /l 1ml。研究的第二阶段在四组蜜蜂身上进行，持续了30天。对照(I)组每日饲喂50% SS 1 ml和H2O 1 ml;II组- SS 1ml +柠檬酸盐0.04 mg /l 1ml;III组- SS 1ml +柠檬酸盐0.02 mg /l 1ml;IV组:SS 1ml +柠檬酸盐0.01 mg /l 1ml。第一阶段，III ~ V组蜜蜂血淋巴α1-球蛋白含量降低。β-球蛋白含量在II (P <)中升高;0.001)， III (P <0.01)， IV (P <0.001)和V (P <0.001)实验组。ⅱ型大鼠血淋巴中γ-球蛋白含量降低;0.001)和III (P <0.01)组。在第二阶段，与对照组相比，II、III和IV实验组的蜜蜂血淋巴中α1 -球蛋白含量降低。IV组蜜蜂血淋巴中α2-球蛋白含量显著降低，β-球蛋白含量显著升高。II (P <)中γ-球蛋白含量增加;0.05)， III和IV (P <0.01)试验组。各实验组蜜蜂血淋巴过氧化氢酶活性均较高(P <0.001)。过氧化氢酶活性以IV组最高。在糖浆饲喂的基础上添加0.01 mg柠檬酸mg，改变了个体血淋巴蛋白组分的比例。白蛋白和β-球蛋白的相对含量降低，α2和γ球蛋白的相对含量升高。0.04 Mg柠檬酸盐可引起各组蜜蜂血淋巴α1相对含量升高，β-和γ-球蛋白相对含量降低。
{"title":"The content of total protein and its fractions in the hemolymph and body tis-sues of bees fed with Mg citrate","authors":"I. I. Kovalchuk, R. L. Androshulik, A. Z. Pylypets, M. M. Tsap","doi":"10.32718/nvlvet11114","DOIUrl":"https://doi.org/10.32718/nvlvet11114","url":null,"abstract":"Mineral elements increase the capacity of organisms for their adaptation to abiotic factors and improve the biological value of animal products. The aim of the experiment was to study the effect of magnesium citrate on the content of total protein in the tissues of the whole organism, the content of soluble protein fractions in hemolymph, and catalase activity. The research was conducted on Carpathian honey bees. They were selected in the apiary at the Institute of Animal Biology of the National Academy of Sciences. The research was conducted in two stages. The first stage of the work was carried out on 5 groups of bees under the conditions of a laboratory thermostat for 20 days. Bees of the control group were fed daily with 1 ml of 50 % sugar syrup (SS) and 1 ml of H2O; group II – 1 ml of SS + 1 ml of 0.4 mg Mg/l nanocitrate; group III – 1 ml of CS + 1 ml of 2 mg Mg/l citrate; group IV – 1 ml of SS + 1 ml of 3 mg Mg/l citrate; group V – 1 ml of SS + 1 ml of 4 mg Mg/l citrate. The second stage of the study was conducted on four groups of bees and lasted 30 days. Bees of the control (I) group were fed daily with 1 ml of 50 % SS and 1 ml of H2O; group II – 1 ml of SS + 1 ml of 0.04 mg Mg/l citrate; group III – 1 ml of SS + 1 ml of 0.02 mg Mg/l citrate; group IV – 1 ml of SS + 1 ml of 0.01 mg Mg/l citrate. At the first stage, a decrease in α1-globulins in the hemolymph of bees of the III – V groups was established. The content of β-globulins increased in the II (P < 0.001), III (P < 0.01), IV (P < 0.001) and V (P < 0.001) experimental groups. The content of γ-globulins decreased in hemolymph of II (P < 0.001) and III (P < 0.01) groups. At the second stage, a decrease in α1–globulins was observed in the hemolymph of bees of the II, III and IV experimental groups compared to the control. The content of α2-globulins was significantly lower in the hemolymph of bees from IV group, and the content of β-globulins was higher in the hemolymph of the bees. An increase in the content of γ-globulins was established in II (P < 0.05), III and IV (P < 0.01) experimental groups. High catalase activity was observed in the hemolymph of bees of all experimental groups (P < 0.001). The highest catalase activity was registered in bees of IV group. The use of 0.01 mg of Mg citrate in addition to sugar syrup feeding changed the ratio of individual hemolymph protein fractions. The relative content of albumin and β-globulin decreased and α2 and γ globulin content increased. An increase in the relative content of α1 and a decrease in β- and γ-globulins in the hemolymph of honey bees of the research groups were caused by Mg citrate in a dose of 0.04 mg.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. M. Sachuk, B. V. Gutyj, Ya. S. Stravskyy, O. A. Katsaraba, O. V. Dyshkant, L. V. Kalynovska
Laboratory studies were conducted to determine the subacute toxicity and tolerance of the veterinary drug “BTF plus” on white rats, dogs, and guinea pigs. The “BTF plus” preparation is a complex vitamin and mineral preparation based on butophosphane, L-carnitine, and cyanocobalamin, which normalizes and corrects metabolic processes in animals and poultry. The drug is used for various types of animals and poultry as a stimulating, tonic and general strengthening agent for obstetric pathologies (difficult births, postpartum complications, paresis, eclampsia, sexual cycle disorders); metabolic disorders caused by irrational feeding, malnutrition, asthenic syndrome, etc.; anemia with helminthiasis; secondary anemias, as an additional means in the treatment of magnesium and calcium deficiency; to increase muscle activity, with significant loads, overstrain and exhaustion in animals; to increase the body's resistance to various pathogens; to stimulate growth, development and live weight gain in young animals and poultry; as an additional means in the treatment of diseases caused by various factors (infectious and non-infectious origin). When administered subcutaneously to rats, the drug “BTF plus” under the conditions of a subacute toxicological experiment in doses (by absolute weight of the drug) of 200.0, 1000.0, and 2000.0 mg/kg of body weight does not cause hemo-, hepato- and nephrotoxic effects on the body of laboratory animals, but on the contrary, stimulates hematopoietic processes and has a positive effect on metabolic processes in their body. Subcutaneous administration of the drug “BTF plus” to dogs in doses (by absolute weight of the drug) of 200.0, 1000.0, and 2000 mg/kg of body weight for ten days does not cause hepatotoxic and nephrotoxic effects on the body under the conditions of a subacute toxicological experiment, but on the contrary, stimulates hematopoietic processes and has a positive effect on metabolic processes in the body of target animals. The tolerance of guinea pigs to the drug “BTF plus” was studied. It was established that the drug does not hurt the body and behavior of ants when administered subcutaneously in doses (based on the absolute weight of the drug) (5000,0–15000,0) mg/kg of body weight. Further studies will be the next stage of pre-registration tests aimed at studying the embryotoxic and carcinogenic effect of “BTF plus”, which is mandatory material of the “Safety and residue studies” section of the dossier for this medicinal product.
{"title":"Research on the specific toxicity of the drug “BTF plus” – a means for nor-malizing metabolic processes in animals and poultry","authors":"R. M. Sachuk, B. V. Gutyj, Ya. S. Stravskyy, O. A. Katsaraba, O. V. Dyshkant, L. V. Kalynovska","doi":"10.32718/nvlvet11106","DOIUrl":"https://doi.org/10.32718/nvlvet11106","url":null,"abstract":"Laboratory studies were conducted to determine the subacute toxicity and tolerance of the veterinary drug “BTF plus” on white rats, dogs, and guinea pigs. The “BTF plus” preparation is a complex vitamin and mineral preparation based on butophosphane, L-carnitine, and cyanocobalamin, which normalizes and corrects metabolic processes in animals and poultry. The drug is used for various types of animals and poultry as a stimulating, tonic and general strengthening agent for obstetric pathologies (difficult births, postpartum complications, paresis, eclampsia, sexual cycle disorders); metabolic disorders caused by irrational feeding, malnutrition, asthenic syndrome, etc.; anemia with helminthiasis; secondary anemias, as an additional means in the treatment of magnesium and calcium deficiency; to increase muscle activity, with significant loads, overstrain and exhaustion in animals; to increase the body's resistance to various pathogens; to stimulate growth, development and live weight gain in young animals and poultry; as an additional means in the treatment of diseases caused by various factors (infectious and non-infectious origin). When administered subcutaneously to rats, the drug “BTF plus” under the conditions of a subacute toxicological experiment in doses (by absolute weight of the drug) of 200.0, 1000.0, and 2000.0 mg/kg of body weight does not cause hemo-, hepato- and nephrotoxic effects on the body of laboratory animals, but on the contrary, stimulates hematopoietic processes and has a positive effect on metabolic processes in their body. Subcutaneous administration of the drug “BTF plus” to dogs in doses (by absolute weight of the drug) of 200.0, 1000.0, and 2000 mg/kg of body weight for ten days does not cause hepatotoxic and nephrotoxic effects on the body under the conditions of a subacute toxicological experiment, but on the contrary, stimulates hematopoietic processes and has a positive effect on metabolic processes in the body of target animals. The tolerance of guinea pigs to the drug “BTF plus” was studied. It was established that the drug does not hurt the body and behavior of ants when administered subcutaneously in doses (based on the absolute weight of the drug) (5000,0–15000,0) mg/kg of body weight. Further studies will be the next stage of pre-registration tests aimed at studying the embryotoxic and carcinogenic effect of “BTF plus”, which is mandatory material of the “Safety and residue studies” section of the dossier for this medicinal product.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"207 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The use of probiotics in animal husbandry ensures the maintenance of homeostasis of the digestive tract and prevents the development of factor infections in young animals – colibacteriosis, dyspepsia, and others, promotes the growth of animals with high survival rates. The main advantages of probiotics over chemotherapeutic drugs and antibiotics are that they are harmless to animal organism and are ecologically clean. In connection with the great attention paid to probiotics as ecologically safe drugs, the study of biological properties and the selection of bacterial strains, the most promising in the probiotic sense, are currently being intensified. This is the direction of the selection of species-specific strains for the intestinal biocenosis of a specific animal species, which have high colonization and antagonistic properties. Therefore, the development of the scientific basis for the creation of new probiotic preparations gave an impetus to their improvement and continued research in this direction. The aim of the work was to study the effect of the probiotic “Bioseven” on the intestinal biocenosis of white rats depending on the dose. Objectives of the study: to establish the toxicity of the drug “Bioseven” on laboratory animals with a single dose (“acute toxicity”) according to microbiological indicators; to investigate the toxicity of “Bioseven” on laboratory animals with long-term administration (“subacute toxicity”) according to microbiological indicators. Research work was carried out during 2022 at the department of veterinary and sanitary examination, hygiene of animal husbandry products and pathanatomy named after J. S. Zagaevskii. The toxicological characterization of the probiotic drug “Bioseven” (study of subacute and acute toxicity) was carried out under the conditions of the State Research Control Institute of Veterinary Medicines and Feed Additives (Laboratory of Pharmacology and Toxicology), (Lviv), the manufacturer of the drug was PP “BTU-CENTER” Ladyzhyn, Vinnytsia region. On the 31st day of use of the drug “Bioseven” (a feed additive with probiotic action) in a therapeutic dose, microorganisms of the genus Lactobacillus were sown 0.3 lg CFU/g more from the large intestine of experimental rats, microorganisms of the genus Bifidobacterium were sown at 0.5 lg CFU/g more than in the control group. The number of Candida fungi decreased by 0.6 lg CFU/g, and the total number of Escherichia coli – 0.2 lg CFU/g. When using the “Bioseven” probiotic in a 5-fold therapeutic dose, 0.5 lg CFU/g more microorganisms of the Lactobacillus genus and 0.6 lg CFU/g more Bifidobacterium microorganisms were sown from the large intestine of experimental rats than in the control group. At the same time, the number of Candida fungi decreased by 0.7 lg CFU/g, and the total number of Escherichia coli to 0.4 lg CFU/g. Under the conditions of use of the drug “Bioseven” in a 10-fold therapeutic dose, microorganisms of the genus Lactobacillus were sown fr
在畜牧业中使用益生菌确保了消化道稳态的维持，并防止了幼龄动物中大肠杆菌病、消化不良等因素感染的发展，促进了动物的生长，提高了动物的存活率。与化疗药物和抗生素相比，益生菌的主要优点是对动物机体无害，生态清洁。随着益生菌作为一种生态安全的药物受到越来越多的关注，益生菌生物学特性的研究和最有前途的菌株的选择正在加强。这是选择具有高度定植和拮抗特性的特定动物物种肠道生物病的物种特异性菌株的方向。因此，创造新的益生菌制剂的科学基础的发展，推动了它们的改进和继续朝这个方向研究。本研究旨在研究益生菌“百乐七”在不同剂量下对大鼠肠道微生物病的影响。研究目的:根据微生物指标，确定单次给药“Bioseven”对实验动物的毒性(“急性毒性”);根据微生物指标调查“百乐七”对实验动物长期给药的毒性(“亚急性毒性”)。研究工作于2022年在以J. S. Zagaevskii命名的兽医和卫生检查、畜牧产品卫生和病理学部门进行。益生菌药物“Bioseven”的毒理学表征(亚急性和急性毒性研究)在国家兽药和饲料添加剂研究控制研究所(药理学和毒理学实验室)(利沃夫)的条件下进行，该药物的制造商是PP“BTU-CENTER”Ladyzhyn, Vinnytsia地区。在使用治疗剂量的“百乐七”(一种具有益生菌作用的饲料添加剂)的第31天，实验大鼠大肠中乳杆菌属微生物的播种量比对照组多0.3 lg CFU/g，双歧杆菌属微生物的播种量比对照组多0.5 lg CFU/g。念珠菌总数减少0.6 lg CFU/g，大肠杆菌总数减少0.2 lg CFU/g。当以5倍治疗剂量使用“Bioseven”益生菌时，实验大鼠大肠内的乳酸杆菌属微生物比对照组多0.5 lg CFU/g，双歧杆菌微生物多0.6 lg CFU/g。同时，念珠菌数量减少了0.7 lg CFU/g，大肠杆菌总数减少了0.4 lg CFU/g。在以10倍治疗剂量使用“百乐七”药物的条件下，实验大鼠大肠内播种的乳杆菌属微生物比对照组多0.9 lg CFU/g，双歧杆菌属微生物比对照组多1.2 lg CFU/g，同时念珠菌数量减少1.1 lg CFU/g，大肠杆菌总数减少0.8 lg CFU/g。进一步研究的前景在于研究生物药物“Bioseven”对农场动物代谢过程和生产品质指标的影响。
{"title":"Influence of the probiotic “Bioseven” on the intestinal biocenose of white rats","authors":"A. V. Dyuba, V. P. Lyasota","doi":"10.32718/nvlvet11117","DOIUrl":"https://doi.org/10.32718/nvlvet11117","url":null,"abstract":"The use of probiotics in animal husbandry ensures the maintenance of homeostasis of the digestive tract and prevents the development of factor infections in young animals – colibacteriosis, dyspepsia, and others, promotes the growth of animals with high survival rates. The main advantages of probiotics over chemotherapeutic drugs and antibiotics are that they are harmless to animal organism and are ecologically clean. In connection with the great attention paid to probiotics as ecologically safe drugs, the study of biological properties and the selection of bacterial strains, the most promising in the probiotic sense, are currently being intensified. This is the direction of the selection of species-specific strains for the intestinal biocenosis of a specific animal species, which have high colonization and antagonistic properties. Therefore, the development of the scientific basis for the creation of new probiotic preparations gave an impetus to their improvement and continued research in this direction. The aim of the work was to study the effect of the probiotic “Bioseven” on the intestinal biocenosis of white rats depending on the dose. Objectives of the study: to establish the toxicity of the drug “Bioseven” on laboratory animals with a single dose (“acute toxicity”) according to microbiological indicators; to investigate the toxicity of “Bioseven” on laboratory animals with long-term administration (“subacute toxicity”) according to microbiological indicators. Research work was carried out during 2022 at the department of veterinary and sanitary examination, hygiene of animal husbandry products and pathanatomy named after J. S. Zagaevskii. The toxicological characterization of the probiotic drug “Bioseven” (study of subacute and acute toxicity) was carried out under the conditions of the State Research Control Institute of Veterinary Medicines and Feed Additives (Laboratory of Pharmacology and Toxicology), (Lviv), the manufacturer of the drug was PP “BTU-CENTER” Ladyzhyn, Vinnytsia region. On the 31st day of use of the drug “Bioseven” (a feed additive with probiotic action) in a therapeutic dose, microorganisms of the genus Lactobacillus were sown 0.3 lg CFU/g more from the large intestine of experimental rats, microorganisms of the genus Bifidobacterium were sown at 0.5 lg CFU/g more than in the control group. The number of Candida fungi decreased by 0.6 lg CFU/g, and the total number of Escherichia coli – 0.2 lg CFU/g. When using the “Bioseven” probiotic in a 5-fold therapeutic dose, 0.5 lg CFU/g more microorganisms of the Lactobacillus genus and 0.6 lg CFU/g more Bifidobacterium microorganisms were sown from the large intestine of experimental rats than in the control group. At the same time, the number of Candida fungi decreased by 0.7 lg CFU/g, and the total number of Escherichia coli to 0.4 lg CFU/g. Under the conditions of use of the drug “Bioseven” in a 10-fold therapeutic dose, microorganisms of the genus Lactobacillus were sown fr","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135369307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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