S. Mykhailiutenko, O. Kruchynenko, J. Serdioucov, O. Klymenko, J. D. Popovytch
Pathomorphological changes, developing in case of pasalurosis chronic course in rabbits are described in the article. The research was held on ten rabbits of Large Grey breed, which were held on household farms of Poltava region. The diagnosing was complex: based on anamnesis data, clinical symptoms’ analysis, and the results of pathomorphological examination. The fragments of the liver, spleen, and kidneys were taken for histological analysis. While making histological analysis of the spleen it was revealed that lymphoid nodules were enlarged, some of them were of uniform structure, and others were in the state of hyperplasia. Non-uniformity of structure in addition to changing the size was registered in those nodules: there was less concentration of lymphoid cells in their central part and more concentration on the periphery. The situation was contrary in separate lymphoid nodules. The red pulp consisted of a large number of lymphoid cells and erythrocytes, the largest number of which were registered in the zones adjoining the places of lymphoid nodules’ location. Sometimes there were megakaryocytes in the field of vision. It was established, that blood filling of arteries was less than normal. In case of using some preparations, bilirubin inclusions were found at having big enlargements in red pulp. Grainy and fatty hepatocytes’ dystrophies, and also lymphohistiocytic interstitial hepatitis were also registered. At the same time, renal corpuscles were enlarged. Rather big spaces, filled with transparent substance containing vessel and cellular fragments (detritus), were revealed in the majority of them between glomeruli and the capsule.Capsule epithelial cells were destroyed in some places. Petechial hemorrhages were registered inside glomeruli. The destruction of separate podocytes and deep cells was noticed. The tubular epithelial cells were enlarged in size, and in some cases they were of cylinder, but not cubic shape; the spaces of such tubular were becoming correspondingly less. The epithelial cell cytoplasm of the tubular in some cases was not uniformand it was of cloudy-grey color: in other cases it had rather large colorless areas and, probably, looked like lipid inclusions (which were washed out from the cells as a result solvent impact during making histological preparations). Concentrations of eosinophilic masses (protein cylinders) sometimes containing certain amount of erythrocytes were often found in canaliculi spaces. Sometimes the destruction of epithelial cells was registered in the tubulеs, and their fragments were found in spaces. As a result of histological examination, the diffuse lymphohistiocytic infiltration in the renal interstitial tissue was found.
{"title":"Pathomorphological changes in parenchymatose organs of rabbits in case of chronic passalurosis","authors":"S. Mykhailiutenko, O. Kruchynenko, J. Serdioucov, O. Klymenko, J. D. Popovytch","doi":"10.32718/NVLVET9306","DOIUrl":"https://doi.org/10.32718/NVLVET9306","url":null,"abstract":"Pathomorphological changes, developing in case of pasalurosis chronic course in rabbits are described in the article. The research was held on ten rabbits of Large Grey breed, which were held on household farms of Poltava region. The diagnosing was complex: based on anamnesis data, clinical symptoms’ analysis, and the results of pathomorphological examination. The fragments of the liver, spleen, and kidneys were taken for histological analysis. While making histological analysis of the spleen it was revealed that lymphoid nodules were enlarged, some of them were of uniform structure, and others were in the state of hyperplasia. Non-uniformity of structure in addition to changing the size was registered in those nodules: there was less concentration of lymphoid cells in their central part and more concentration on the periphery. The situation was contrary in separate lymphoid nodules. The red pulp consisted of a large number of lymphoid cells and erythrocytes, the largest number of which were registered in the zones adjoining the places of lymphoid nodules’ location. Sometimes there were megakaryocytes in the field of vision. It was established, that blood filling of arteries was less than normal. In case of using some preparations, bilirubin inclusions were found at having big enlargements in red pulp. Grainy and fatty hepatocytes’ dystrophies, and also lymphohistiocytic interstitial hepatitis were also registered. At the same time, renal corpuscles were enlarged. Rather big spaces, filled with transparent substance containing vessel and cellular fragments (detritus), were revealed in the majority of them between glomeruli and the capsule.Capsule epithelial cells were destroyed in some places. Petechial hemorrhages were registered inside glomeruli. The destruction of separate podocytes and deep cells was noticed. The tubular epithelial cells were enlarged in size, and in some cases they were of cylinder, but not cubic shape; the spaces of such tubular were becoming correspondingly less. The epithelial cell cytoplasm of the tubular in some cases was not uniformand it was of cloudy-grey color: in other cases it had rather large colorless areas and, probably, looked like lipid inclusions (which were washed out from the cells as a result solvent impact during making histological preparations). Concentrations of eosinophilic masses (protein cylinders) sometimes containing certain amount of erythrocytes were often found in canaliculi spaces. Sometimes the destruction of epithelial cells was registered in the tubulеs, and their fragments were found in spaces. As a result of histological examination, the diffuse lymphohistiocytic infiltration in the renal interstitial tissue was found.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86213797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Today, the actual environmental problem of the agro-industrial complex is the damage to the fodder of vegetable origin, as well as livestock production by mycotoxins – secondary metabolites of mold fungi. Owing to the widespread spread and severe toxic effects on animals and humans, the study of ochratoxins, the main producers of Aspergillus ochraceus and Pennicilium viridicatum, is intensively studied. The pigs are very sensitive to ochratoxins, the effects of which the most pronounced changes develop in the organs of the urinary system, as well as in the gastrointestinal tract, the liver, the immune and nervous systems. Diagnosis of ochratoxicosis should be comprehensive, based on anamnestic data, clinical, pathologoanatomical and chemico-toxicological studies. In the course of the study, the pathoanatomical changes that are developing in the body of sows for ochratoxicosis are studied in detail. Also, a mycological and mycotoxicological study was conducted in which the producer of ochertoxins Aspergillus ochraceus was detected in feeds. The content of ochratoxin A in feed fed to sows varied from 8.32 mg/kg to 85.72 mg/kg, and in the kidneys its concentration was 4.34–48.33 mg/kg. In a pathologoanatomical study in the kidneys, there was a discovery of gialinosis and sclerosis of renal glomeruli, as well as hyaline droplets degeneration, necrotic changes in epithelial proximal tubule, infiltration of interstitium by lymphocytes, macrophages, fibroblasts, focal growth of connective tissue, which was accompanied by the appearance of cystic cavities in 21.4% of animals. In the gastrointestinal tract, acute catarrhal or catarrhal-hemorrhagic inflammation was detected, and in 28.57% of sows the ulcers of the fundus zone of the mucous membrane of the stomach, the development of which was accompanied by massive hemorrhage in the gastrointestinal tract and posthemorrhagic anemia. Alterative (necrotic and degenerative changes) of hepatocytes, which were most pronounced in the periportal zone of the liver lobes, as well as acute congestive hyperemia, stasis in vessels of the microcirculatory bed, focal infiltration of the stroma by lymphocytes and histiocytes, were recorded in the liver. In the peripheral organs of the immune system, necrotic changes in lymphoid cells were recorded, indicating an immunosuppressive effect of ochratoxins.
{"title":"Pathomorphologic changes of pigs by the spontaneous ochratoxicosis","authors":"R. Dankovych","doi":"10.32718/NVLVET9323","DOIUrl":"https://doi.org/10.32718/NVLVET9323","url":null,"abstract":"Today, the actual environmental problem of the agro-industrial complex is the damage to the fodder of vegetable origin, as well as livestock production by mycotoxins – secondary metabolites of mold fungi. Owing to the widespread spread and severe toxic effects on animals and humans, the study of ochratoxins, the main producers of Aspergillus ochraceus and Pennicilium viridicatum, is intensively studied. The pigs are very sensitive to ochratoxins, the effects of which the most pronounced changes develop in the organs of the urinary system, as well as in the gastrointestinal tract, the liver, the immune and nervous systems. Diagnosis of ochratoxicosis should be comprehensive, based on anamnestic data, clinical, pathologoanatomical and chemico-toxicological studies. In the course of the study, the pathoanatomical changes that are developing in the body of sows for ochratoxicosis are studied in detail. Also, a mycological and mycotoxicological study was conducted in which the producer of ochertoxins Aspergillus ochraceus was detected in feeds. The content of ochratoxin A in feed fed to sows varied from 8.32 mg/kg to 85.72 mg/kg, and in the kidneys its concentration was 4.34–48.33 mg/kg. In a pathologoanatomical study in the kidneys, there was a discovery of gialinosis and sclerosis of renal glomeruli, as well as hyaline droplets degeneration, necrotic changes in epithelial proximal tubule, infiltration of interstitium by lymphocytes, macrophages, fibroblasts, focal growth of connective tissue, which was accompanied by the appearance of cystic cavities in 21.4% of animals. In the gastrointestinal tract, acute catarrhal or catarrhal-hemorrhagic inflammation was detected, and in 28.57% of sows the ulcers of the fundus zone of the mucous membrane of the stomach, the development of which was accompanied by massive hemorrhage in the gastrointestinal tract and posthemorrhagic anemia. Alterative (necrotic and degenerative changes) of hepatocytes, which were most pronounced in the periportal zone of the liver lobes, as well as acute congestive hyperemia, stasis in vessels of the microcirculatory bed, focal infiltration of the stroma by lymphocytes and histiocytes, were recorded in the liver. In the peripheral organs of the immune system, necrotic changes in lymphoid cells were recorded, indicating an immunosuppressive effect of ochratoxins.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85455308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Nechyporenko, A. V. Berezovskyy, T. Fotina, R. Petrov
The article presents data on the study of corrosion activity and foaming properties of biocide “DezSan”. One of the main characteristics of a biocide is its corrosive activity. The use of a biocide in a poultry house involves getting it on metal, rubber, plastic surfaces, and the like. The biocide should provide minimal aggressive effect on the surface data. In the industrial poultry industry, the most frequently used equipment with surfaces made of stainless steel, aluminum and galvanized iron. Also, when choosing a biocide, pay attention to its foaming properties. They should not exceed 40%, as this ensures a quality disinfection. One of the key points is the index of foam stability, which significantly affects the time of contact with the biocide and provides effective decontamination of the surfaces. The goal of the work. To determine the corrosion activity and foaming properties of the new domestic biocide “DezSan”. Materials and methods of research. In their experiments, a disinfection tool for the production of the scientific and production company “Brovаfarma” (Ukraine) “DesSan” was used. Relative corrosion activity of various concentrations of biocide “DezSan” was determined in comparison with the standard – 1.5% solution of sodium hydroxide. To determine the foaming properties of biocide “DezSan” used the method of Ross-Miles (1983). Results of research and discussion. The biocide “DezSan” in all experimented concentrations (0.25–1.5%) has a minor corrosion effect on aluminum, stainless and galvanized steel, compared with the standard (1.5% NaOH solution). The biocide “DezSan” does not cause deformation when exposed to metal samples. “DezSan” in various concentrations has a foaming property of 13.1 to 37.3%, which increased directly in proportion to increasing the concentration of biocides. The foaming capacity of “DezSan” did not exceed 40%, and the foam stability at these concentrations varied within 0,08–0,19. Conclusions and prospects for further research. Thus, the biocide “DezSan” has low corrosion properties in relation to aluminum, stainless steel and galvanized steel in comparison with the standard (1.5% solution of NaOH). The biocide “DezSan” does not cause deformation when exposed to metal samples. The foaming capacity was not more than 40%, and the foam resistance was up to 0.19. These indicators allow the wide use of biocide “DezSan” in the poultry industry. In the future, it is planned to conduct a study of the biocid “DezSan”, identified it irritating, skin-resorptive, cumulative and other properties.
{"title":"Investigation of corrosion activity and foaming properties of biocide “DezSan”","authors":"O. Nechyporenko, A. V. Berezovskyy, T. Fotina, R. Petrov","doi":"10.32718/NVLVET9315","DOIUrl":"https://doi.org/10.32718/NVLVET9315","url":null,"abstract":"The article presents data on the study of corrosion activity and foaming properties of biocide “DezSan”. One of the main characteristics of a biocide is its corrosive activity. The use of a biocide in a poultry house involves getting it on metal, rubber, plastic surfaces, and the like. The biocide should provide minimal aggressive effect on the surface data. In the industrial poultry industry, the most frequently used equipment with surfaces made of stainless steel, aluminum and galvanized iron. Also, when choosing a biocide, pay attention to its foaming properties. They should not exceed 40%, as this ensures a quality disinfection. One of the key points is the index of foam stability, which significantly affects the time of contact with the biocide and provides effective decontamination of the surfaces. The goal of the work. To determine the corrosion activity and foaming properties of the new domestic biocide “DezSan”. Materials and methods of research. In their experiments, a disinfection tool for the production of the scientific and production company “Brovаfarma” (Ukraine) “DesSan” was used. Relative corrosion activity of various concentrations of biocide “DezSan” was determined in comparison with the standard – 1.5% solution of sodium hydroxide. To determine the foaming properties of biocide “DezSan” used the method of Ross-Miles (1983). Results of research and discussion. The biocide “DezSan” in all experimented concentrations (0.25–1.5%) has a minor corrosion effect on aluminum, stainless and galvanized steel, compared with the standard (1.5% NaOH solution). The biocide “DezSan” does not cause deformation when exposed to metal samples. “DezSan” in various concentrations has a foaming property of 13.1 to 37.3%, which increased directly in proportion to increasing the concentration of biocides. The foaming capacity of “DezSan” did not exceed 40%, and the foam stability at these concentrations varied within 0,08–0,19. Conclusions and prospects for further research. Thus, the biocide “DezSan” has low corrosion properties in relation to aluminum, stainless steel and galvanized steel in comparison with the standard (1.5% solution of NaOH). The biocide “DezSan” does not cause deformation when exposed to metal samples. The foaming capacity was not more than 40%, and the foam resistance was up to 0.19. These indicators allow the wide use of biocide “DezSan” in the poultry industry. In the future, it is planned to conduct a study of the biocid “DezSan”, identified it irritating, skin-resorptive, cumulative and other properties.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"528 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79636346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Predators is medium-sized and large mammal leading a terrestrial way of life. They are mostly carnivorous animals, the nature of nutrition of which indicate certain devices are reflected in the structure of the scull. The aim of the work is to conduct a comparative anatomical study of the structure of the scull and to determine the craniometric indicators in animals of families Feline (Domestic cat), Canine (Domestic dog, Wolf, Fox ordinary) and Mustelidae (stone marten, forest polecat, badger ordinary) a squad of Carnivore. Comparative anatomical, craniological and statistical methods of research were used. It was found that the scull of test animals is of a General anatomical structure (wide temporal fossa, a deep masseteric fossa, well-defined nuchal and external sagittal crests, the presence of angular process on mandible, relatively large tympanic bullas, underdeveloped lacrimal bone) and species differences (topography of split of the external sagittal crest, shape of mandibular angular process, the presence of intratympanical part of the temporal bone). Absolute craniometrical indices of badger more of indicators of marten, since they directly depend on the weight and body size of these animals. There are most vary the overall length of the scull (1.69 times; P < 0.001), length of bony palate (1.82 times; P < 0.001), length of the external sagittal crest (3.67 times; P < 0.001), width of bony palate at the level of the first premolar and the last molar (2.33 times; P < 0.001), width of choans on the level of hook-shaped processes of pterygoid bone (1.75 times; P < 0.01), width of choans at the level of the caudal edge of the Palatine bone (2 times; P < 0.05), the total height of the scull (2.36 times; P < 0.001). Most of the relative craniometric indicators of the badger scull, compared with such in martens, are almost the same, due to the close specificity of nutrition and environmental characteristics in natural biocenosis. Due to the well-developed external sagittal crest, the height of the brain relative to the total height of the scull in badger (61.02 ± 4.73%) is 1.51 times less (P < 0.001) than in marten (92 ± 6.12%). The relative length of the external sagittal crest to the total length of the badger scull is 2.17 times longer (P < 0.001) (53.1 ± 3.09 versus 24.42 ± 1.41%), compared with marten, due to the more powerful development of masticatory muscles, temporal in particular.
{"title":"The structural features of the sculls of animals of a series of Carnivore","authors":"T. Kot, I. Lutsiuk, O. V. Synytskyi","doi":"10.32718/NVLVET9312","DOIUrl":"https://doi.org/10.32718/NVLVET9312","url":null,"abstract":"Predators is medium-sized and large mammal leading a terrestrial way of life. They are mostly carnivorous animals, the nature of nutrition of which indicate certain devices are reflected in the structure of the scull. The aim of the work is to conduct a comparative anatomical study of the structure of the scull and to determine the craniometric indicators in animals of families Feline (Domestic cat), Canine (Domestic dog, Wolf, Fox ordinary) and Mustelidae (stone marten, forest polecat, badger ordinary) a squad of Carnivore. Comparative anatomical, craniological and statistical methods of research were used. It was found that the scull of test animals is of a General anatomical structure (wide temporal fossa, a deep masseteric fossa, well-defined nuchal and external sagittal crests, the presence of angular process on mandible, relatively large tympanic bullas, underdeveloped lacrimal bone) and species differences (topography of split of the external sagittal crest, shape of mandibular angular process, the presence of intratympanical part of the temporal bone). Absolute craniometrical indices of badger more of indicators of marten, since they directly depend on the weight and body size of these animals. There are most vary the overall length of the scull (1.69 times; P < 0.001), length of bony palate (1.82 times; P < 0.001), length of the external sagittal crest (3.67 times; P < 0.001), width of bony palate at the level of the first premolar and the last molar (2.33 times; P < 0.001), width of choans on the level of hook-shaped processes of pterygoid bone (1.75 times; P < 0.01), width of choans at the level of the caudal edge of the Palatine bone (2 times; P < 0.05), the total height of the scull (2.36 times; P < 0.001). Most of the relative craniometric indicators of the badger scull, compared with such in martens, are almost the same, due to the close specificity of nutrition and environmental characteristics in natural biocenosis. Due to the well-developed external sagittal crest, the height of the brain relative to the total height of the scull in badger (61.02 ± 4.73%) is 1.51 times less (P < 0.001) than in marten (92 ± 6.12%). The relative length of the external sagittal crest to the total length of the badger scull is 2.17 times longer (P < 0.001) (53.1 ± 3.09 versus 24.42 ± 1.41%), compared with marten, due to the more powerful development of masticatory muscles, temporal in particular.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79859781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Increasing food production and improving their quality is one of the main problems facing the agro-industrial complex of Ukraine in modern conditions. The solution of this problem is impossible only by increasing the production of livestock products, although this is the main source of their receipt. It is necessary to use additional, non-traditional types of raw materials containing full proteins, fats, vitamins, minerals and other biologically active substances necessary for the human body. One of these sources may be meat of food types of snails. It maintains a complex of replaceable and essential amino acids, vitamins, inorganic elements, carbohydrates and fats. The purpose of our work was to determine the content of fatty acids in cooked meat of the food species Helix pomatia, Helix aspersa maxima and Helix aspersa muller. This article presents the results of the content of fatty acids in meat of food snails using the method of gas chromatography. The presence and quantity of fatty acids is determined in three types of snails. In general, the meat of snails contains both saturated and unsaturated fatty acids. The composition of saturated fatty acids includes – kapron, palmithin, stearin, myristic, arachin, capryl, laurin, begen, kaprin, heptadecanova. From unsaturated fatty acids, snail meat contains omega-3, omega-6, oleinic, palmitoleic, linoleic, arachidonic, linolenic, cis-5,8,11,14,17-eicosapentaenoic, cis-11,14-eicosadenic, cis-13,16-doceta-adenoic, docosapentaenoic. As a result of our research on the content of fatty acids in cooked meat of snails, we found that the mass fraction of 11 unsaturated fatty acids – oleinic, palmitoleic, linoleic, omega-3, omega-6, arachidonic, linolenic, cis-5,8,11, 14,17-eicosapentaenoic, cis-11,14-eicosadenic, cis-13,16-doceta-adenoic, docosapentaenoic to a mass fraction of 10 saturated fatty acids is significantly different in each type of snail. Thus, the mass fraction of saturated to unsaturated fatty acids is in the meat of Helix pomatia, Helix aspersa maxima, Helix aspersa muller 1:5.73; 1:5.05 and 1:4.81 respectively. Most of all, unsaturated fatty acids are found in boiled meat of Helix pomatia snails and amount to 126.77% to the sum of fatty acids, and saturated – in the boiled meat of Helix aspersa muller snails – 24.86% to the sum of fatty acids. Useful and well-known all fatty acids ω-3 and ω-6 enriched boiled meat of Helix aspersa maxima snails – 49.06% to the sum of fatty acids, while Helix pomatia and Helix aspersa muller are 48.81 and 44.35% to the amount of fatty acids, respectively. It should be noted that the total content of fatty acids in the meat of snails varies in the following limits: Helix pomatia snails 148.89, Helix aspersa maxima 149.11, and Helix aspersa muller 144.4. Thus it can be argued that in the boiled meat of the three types of snails we studied, which is Helix pomatia, Helix aspersa maxima and Helix aspersa muller can be used as a valuable source of saturated and uns
增加粮食产量和提高粮食质量是乌克兰农工综合体在现代条件下面临的主要问题之一。要解决这一问题，仅靠增加畜产品的生产是不可能的，尽管这是畜产品收入的主要来源。有必要使用额外的、非传统类型的原料，这些原料含有人体所需的全部蛋白质、脂肪、维生素、矿物质和其他生物活性物质。其中一种食物来源可能是蜗牛的肉。它维持着可替代的和必需的氨基酸、维生素、无机元素、碳水化合物和脂肪的复合物。本研究的目的是测定熟肉中脂肪酸的含量，其中熟肉中脂肪酸的含量主要有三种，一种是梨形螺旋，另一种是大梨形螺旋。本文介绍了用气相色谱法测定食用蜗牛肉中脂肪酸含量的结果。测定了三种蜗牛体内脂肪酸的含量和含量。一般来说，蜗牛肉中含有饱和脂肪酸和不饱和脂肪酸。饱和脂肪酸的组成包括- kapon、棕榈素、硬脂素、肉豆蔻、花生素、capyl、laurin、begen、kaprin、heptadanova。从不饱和脂肪酸中，蜗牛肉含有omega-3、omega-6、油酸、棕榈油酸、亚油酸、花生四烯酸、亚麻酸、顺式-5、8、11、14、17-二十碳五烯酸、顺式-11、14-二十碳五烯酸、顺式-13、16-二十碳五烯酸、二十碳五烯酸。通过对蜗牛熟肉中脂肪酸含量的研究，我们发现11种不饱和脂肪酸——油酸、棕榈油酸、亚油酸、omega-3、omega-6、花生四烯酸、亚麻酸、顺式5、8、11、14、17-二十碳五烯酸、顺式11、14-二十碳五烯酸、顺式13、16-doceta-腺酸、二十碳五烯酸与10种饱和脂肪酸的质量分数在不同种类蜗牛中存在显著差异。因此，饱和脂肪酸与不饱和脂肪酸的质量分数为:1:5.73;分别是1:5.05和1:4.81。其中，水煮螺肉中不饱和脂肪酸占脂肪酸总量的126.77%，水煮螺肉中饱和脂肪酸占脂肪酸总量的24.86%。螺蛳煮肉中有益脂肪酸ω-3和ω-6的含量为49.06%，螺蛳煮肉中脂肪酸含量为48.81%，螺蛳煮肉中脂肪酸含量为44.35%。需要注意的是，蜗牛肉中脂肪酸的总含量在以下范围内变化:螺旋pomatia蜗牛148.89，螺旋aspersa maxima 149.11，螺旋aspersa muller 144.4。因此，我们可以认为，在我们所研究的三种蜗牛的煮肉中，螺旋pomatia，螺旋aspersa maxima和螺旋aspersa muller可以作为饱和和不饱和脂肪酸的宝贵来源。
{"title":"The content of fatty acids in the meat of snails by the action of heat processing","authors":"I. Danilova","doi":"10.32718/NVLVET9305","DOIUrl":"https://doi.org/10.32718/NVLVET9305","url":null,"abstract":"Increasing food production and improving their quality is one of the main problems facing the agro-industrial complex of Ukraine in modern conditions. The solution of this problem is impossible only by increasing the production of livestock products, although this is the main source of their receipt. It is necessary to use additional, non-traditional types of raw materials containing full proteins, fats, vitamins, minerals and other biologically active substances necessary for the human body. One of these sources may be meat of food types of snails. It maintains a complex of replaceable and essential amino acids, vitamins, inorganic elements, carbohydrates and fats. The purpose of our work was to determine the content of fatty acids in cooked meat of the food species Helix pomatia, Helix aspersa maxima and Helix aspersa muller. This article presents the results of the content of fatty acids in meat of food snails using the method of gas chromatography. The presence and quantity of fatty acids is determined in three types of snails. In general, the meat of snails contains both saturated and unsaturated fatty acids. The composition of saturated fatty acids includes – kapron, palmithin, stearin, myristic, arachin, capryl, laurin, begen, kaprin, heptadecanova. From unsaturated fatty acids, snail meat contains omega-3, omega-6, oleinic, palmitoleic, linoleic, arachidonic, linolenic, cis-5,8,11,14,17-eicosapentaenoic, cis-11,14-eicosadenic, cis-13,16-doceta-adenoic, docosapentaenoic. As a result of our research on the content of fatty acids in cooked meat of snails, we found that the mass fraction of 11 unsaturated fatty acids – oleinic, palmitoleic, linoleic, omega-3, omega-6, arachidonic, linolenic, cis-5,8,11, 14,17-eicosapentaenoic, cis-11,14-eicosadenic, cis-13,16-doceta-adenoic, docosapentaenoic to a mass fraction of 10 saturated fatty acids is significantly different in each type of snail. Thus, the mass fraction of saturated to unsaturated fatty acids is in the meat of Helix pomatia, Helix aspersa maxima, Helix aspersa muller 1:5.73; 1:5.05 and 1:4.81 respectively. Most of all, unsaturated fatty acids are found in boiled meat of Helix pomatia snails and amount to 126.77% to the sum of fatty acids, and saturated – in the boiled meat of Helix aspersa muller snails – 24.86% to the sum of fatty acids. Useful and well-known all fatty acids ω-3 and ω-6 enriched boiled meat of Helix aspersa maxima snails – 49.06% to the sum of fatty acids, while Helix pomatia and Helix aspersa muller are 48.81 and 44.35% to the amount of fatty acids, respectively. It should be noted that the total content of fatty acids in the meat of snails varies in the following limits: Helix pomatia snails 148.89, Helix aspersa maxima 149.11, and Helix aspersa muller 144.4. Thus it can be argued that in the boiled meat of the three types of snails we studied, which is Helix pomatia, Helix aspersa maxima and Helix aspersa muller can be used as a valuable source of saturated and uns","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78460107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article presents the results of histological studies of the frontal area of the cerebral cortexб taken from 10 corpses of cats aged from 3 months to 5.5 years, in which infectious peritonitis (based on anamnesis, clinical signs, morphological and biochemical blood analysis , ultrasound diagnostics, Rivalt test and express diagnosis) was diagnosed during their life. A pathoanatomical study was conducted; samples of the frontal area of the cerebral cortex of cats, which were fixed in a 12% aqueous solution of neutral formalin, a solution of Carnoua and 96° ethyl alcohol, were selected. The histocuts were made, which were stained with hematoxylin and eosin, thionine for Nisslem. In order to detect the astrocytic glory, a fraction of the cerebral cortex was fixed by the Golgi-Clatzo method with a fresh mixture of chloral hydrate-formalin-bichromate and produced histocuts on a freezing microtome. For histological examination of the cerebral cortex of cats, changes in the non-inflammatory character were observed, which were characterized by disorders of hemomycrocycle circulation, edema and degenerative processes of the neuroglial complex and neurons. Violations of the structural organization of endothelial cells, basal membranes of capillaries and venules should be considered as an important indicator of functional changes in tone, permeability of the microcirculatory channel, which contributed to the development of perivascular and pericellular edema, which in its turn led to regressive changes of glial elements; acute swelling of neurons with the formation of microcavities in nuclei; a sharp decrease in the content of the chromatophilic substance in pyramidal and stellate cells; the development of gidropic dystrophy in star cells and creatures; rise of shadow cells. Circulatory disorders caused the violation of the trophic transport systems, which caused hypoxia, accumulation of acidic metabolism products, and formed the basis for the pathogenesis of the development of dyscirculatory dystrophy of neuroglial cells and neurons, i.e. neurotrophic disorders of the cerebral cortex of cats for FIR.
{"title":"Pathomorphology of the cerebral cortex of cats for infectious peritonitis","authors":"G. Kotsyumbas, M. Khalaniia","doi":"10.32718/NVLVET9301","DOIUrl":"https://doi.org/10.32718/NVLVET9301","url":null,"abstract":"The article presents the results of histological studies of the frontal area of the cerebral cortexб taken from 10 corpses of cats aged from 3 months to 5.5 years, in which infectious peritonitis (based on anamnesis, clinical signs, morphological and biochemical blood analysis , ultrasound diagnostics, Rivalt test and express diagnosis) was diagnosed during their life. A pathoanatomical study was conducted; samples of the frontal area of the cerebral cortex of cats, which were fixed in a 12% aqueous solution of neutral formalin, a solution of Carnoua and 96° ethyl alcohol, were selected. The histocuts were made, which were stained with hematoxylin and eosin, thionine for Nisslem. In order to detect the astrocytic glory, a fraction of the cerebral cortex was fixed by the Golgi-Clatzo method with a fresh mixture of chloral hydrate-formalin-bichromate and produced histocuts on a freezing microtome. For histological examination of the cerebral cortex of cats, changes in the non-inflammatory character were observed, which were characterized by disorders of hemomycrocycle circulation, edema and degenerative processes of the neuroglial complex and neurons. Violations of the structural organization of endothelial cells, basal membranes of capillaries and venules should be considered as an important indicator of functional changes in tone, permeability of the microcirculatory channel, which contributed to the development of perivascular and pericellular edema, which in its turn led to regressive changes of glial elements; acute swelling of neurons with the formation of microcavities in nuclei; a sharp decrease in the content of the chromatophilic substance in pyramidal and stellate cells; the development of gidropic dystrophy in star cells and creatures; rise of shadow cells. Circulatory disorders caused the violation of the trophic transport systems, which caused hypoxia, accumulation of acidic metabolism products, and formed the basis for the pathogenesis of the development of dyscirculatory dystrophy of neuroglial cells and neurons, i.e. neurotrophic disorders of the cerebral cortex of cats for FIR.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79362090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The main task of the dairy industry is the production of raw milk, the quality of which depends on the degree of bacterial background of milk and is achieved only if the regular cleaning and disinfection of milking equipment, dairy equipment. Milk is a good nutrient for microorganisms. The safety of milk that is obtained in dairy complexes provides for acceptable standards in its composition of any chemical, physical or biological substances that pose a potential threat to human health. The largest share of the listed factors belongs to microbiological contamination. The purpose of the work was to investigate the minimum bactericidal concentration of the acid detergent “Milkodez” on the test culture of microorganisms: S. aureus ATCC 25923, E. coli 055K59 No. 3912/41, P. aeruginosa 27/99. The study found that the acid “Milkodez” has bactericidal activity on the S. aureus culture test at dilution of a disinfectant 1: 268.8 and less and an active substance concentration of 0.371% or more at exposure of 2 minutes, for breeding 1:376, 5 and less (concentration of the active substance 0.265% and more) at exposure of 5 minutes and at dilution 1:1466.3 and less and a concentration of 0.0691% or more for 15 minutes. Minimal bactericidal concentration of the acid “Milkodez” on the test of E. coli culture at an exposure of 2 minutes amounted to 0.134%, at an exposure of 5 minutes. – 0.0493% and 15 minutes – 0.0352%. When the concentration of the agent is 0.012856% or more and its dilution is 1:7778.4 and less, throughout all investigated time ranges, there is no growth of the test culture P. aeruginosa. The use of the product at a temperature of 70 ± 5 °C allowed to inhibit the golden staphylococci at the concentration of the solution of the drug 0.371% for 2 minutes of action and 0.265% and 0.0691% – respectively within 5 and 15 minutes. At the same time, at the temperature of the solution of the Milkodez solution 70 ± 5 °C, the death of the E. coli was observed at the concentration of the acidic substance 0.0352%, 0.0493% and 0.134%, respectively, for 2, 5 and 15 minutes. The increase in the temperature of the solution from 20 ± 5 °C to 70 ± 5 °C resulted in the death of P. aeruginosa bacteria at a concentration of 0.01285% in all time ranges.
{"title":"Investigation of the minimum bactericidal concentration of acid detergent “Milkodez” on the test culture of microorganisms","authors":"M. Verkholiuk","doi":"10.32718/NVLVET9316","DOIUrl":"https://doi.org/10.32718/NVLVET9316","url":null,"abstract":"The main task of the dairy industry is the production of raw milk, the quality of which depends on the degree of bacterial background of milk and is achieved only if the regular cleaning and disinfection of milking equipment, dairy equipment. Milk is a good nutrient for microorganisms. The safety of milk that is obtained in dairy complexes provides for acceptable standards in its composition of any chemical, physical or biological substances that pose a potential threat to human health. The largest share of the listed factors belongs to microbiological contamination. The purpose of the work was to investigate the minimum bactericidal concentration of the acid detergent “Milkodez” on the test culture of microorganisms: S. aureus ATCC 25923, E. coli 055K59 No. 3912/41, P. aeruginosa 27/99. The study found that the acid “Milkodez” has bactericidal activity on the S. aureus culture test at dilution of a disinfectant 1: 268.8 and less and an active substance concentration of 0.371% or more at exposure of 2 minutes, for breeding 1:376, 5 and less (concentration of the active substance 0.265% and more) at exposure of 5 minutes and at dilution 1:1466.3 and less and a concentration of 0.0691% or more for 15 minutes. Minimal bactericidal concentration of the acid “Milkodez” on the test of E. coli culture at an exposure of 2 minutes amounted to 0.134%, at an exposure of 5 minutes. – 0.0493% and 15 minutes – 0.0352%. When the concentration of the agent is 0.012856% or more and its dilution is 1:7778.4 and less, throughout all investigated time ranges, there is no growth of the test culture P. aeruginosa. The use of the product at a temperature of 70 ± 5 °C allowed to inhibit the golden staphylococci at the concentration of the solution of the drug 0.371% for 2 minutes of action and 0.265% and 0.0691% – respectively within 5 and 15 minutes. At the same time, at the temperature of the solution of the Milkodez solution 70 ± 5 °C, the death of the E. coli was observed at the concentration of the acidic substance 0.0352%, 0.0493% and 0.134%, respectively, for 2, 5 and 15 minutes. The increase in the temperature of the solution from 20 ± 5 °C to 70 ± 5 °C resulted in the death of P. aeruginosa bacteria at a concentration of 0.01285% in all time ranges.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80964798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. O. Zhukova, A. A. Molchanov, I. O. Kostiuk, O. Bobrytska, N. I. Lonhus, I. Ionov
Pork production plays a significant role in meat balance of Ukraine. High productivity of pigs is accompanied with increased intensity of metabolic processes and intense functional activity of all organs and systems, which requires additional support. To grow a healthy livestock, it is necessary to use preparations and biologically active forage plant additives that help a body to realize productive qualities, fight against various pathogens, but do not harm the animal body and are safe in the production of meat products for human nutrition. The influence of phytobiotic of the cardiac macleaya (Macleáya cordáta R. Br.) has been investigated separately and in combination with vegetable additives (buckwheat, ground onion and buckwheat husks) as a source of flavonoids on the physiological state and carbohydrate metabolism of pigs. It has been determined that application of only macleaya supplements to the 30 and 60 days of studies leads to an increase in the number of erythrocytes by 16.7% and 23.2% (P < 0.05) and hemoglobin concentration by 30.7% and 37.9% (P < 0.01), as well as activation of anaerobic glycolysis, as evidenced by a significant increase in glucose concentration by 96.4–48.0% (P < 0.01), lactate by 71.4–55.2%, (P < 0.01) and activity of lactate dehydrogenase at 18.6–29.4% (P < 0.05) and reduction of pyruvate content by 34.0–13.4% (P < 0.01) and activity of alkaline phosphatase – by 66.2% and 76.8% (P < 0.01) in the corresponding terms of research. While using macleaya with green buckwheat, buckwheat and onion husks, the hematological parameters and parameters of carbohydrate metabolism were at the level of the control group, confirming the significant decrease in the concentration of glucose and lactate compared with the control and shift ratio lactate / pyruvate towards pyruvate. This fact suggests that bioflavonoids, which are part of plant additives, have not only antioxidant properties, but also serve as a correction and stabilizer of carbohydrate metabolism and mitigate the side hypoxic effects of alkaloids of phytobiotic of the macleaya cordata.
{"title":"The investigation of the influence which is done as a result of macleaya cordata and biologically active additives on the carbohydrate metabolism in pigs","authors":"I. O. Zhukova, A. A. Molchanov, I. O. Kostiuk, O. Bobrytska, N. I. Lonhus, I. Ionov","doi":"10.32718/NVLVET9324","DOIUrl":"https://doi.org/10.32718/NVLVET9324","url":null,"abstract":"Pork production plays a significant role in meat balance of Ukraine. High productivity of pigs is accompanied with increased intensity of metabolic processes and intense functional activity of all organs and systems, which requires additional support. To grow a healthy livestock, it is necessary to use preparations and biologically active forage plant additives that help a body to realize productive qualities, fight against various pathogens, but do not harm the animal body and are safe in the production of meat products for human nutrition. The influence of phytobiotic of the cardiac macleaya (Macleáya cordáta R. Br.) has been investigated separately and in combination with vegetable additives (buckwheat, ground onion and buckwheat husks) as a source of flavonoids on the physiological state and carbohydrate metabolism of pigs. It has been determined that application of only macleaya supplements to the 30 and 60 days of studies leads to an increase in the number of erythrocytes by 16.7% and 23.2% (P < 0.05) and hemoglobin concentration by 30.7% and 37.9% (P < 0.01), as well as activation of anaerobic glycolysis, as evidenced by a significant increase in glucose concentration by 96.4–48.0% (P < 0.01), lactate by 71.4–55.2%, (P < 0.01) and activity of lactate dehydrogenase at 18.6–29.4% (P < 0.05) and reduction of pyruvate content by 34.0–13.4% (P < 0.01) and activity of alkaline phosphatase – by 66.2% and 76.8% (P < 0.01) in the corresponding terms of research. While using macleaya with green buckwheat, buckwheat and onion husks, the hematological parameters and parameters of carbohydrate metabolism were at the level of the control group, confirming the significant decrease in the concentration of glucose and lactate compared with the control and shift ratio lactate / pyruvate towards pyruvate. This fact suggests that bioflavonoids, which are part of plant additives, have not only antioxidant properties, but also serve as a correction and stabilizer of carbohydrate metabolism and mitigate the side hypoxic effects of alkaloids of phytobiotic of the macleaya cordata.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"136 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82993336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The inadequacy of mineral nutrition leads to inhibition of physiological and metabolic reactions in the body of honeybees. It is known that Cobalt chloride is used to activate oviposition of the queen bee. It was established that Cobalt and Nickel citrate, obtained by the method of nanotechnology, corrects the mineral metabolism and affects the metabolism of bees. It is known that Cobalt plays an important role in the work of enzymes; synthesis of vitamin B12, promotes assimilation of vitamins A, E, C; increases protein metabolism, participates in hematopoiesis. Nickel also has a pronounced effect on hemopoiesis, namely on the morphological composition of blood. But its effect on the cell and subcellular level is not well understood. The results of studies of the effect of soybean flour with the addition of Cobalt and Nickel citrates on the content of mineral elements in the tissues of the body of bees and honeycombs are given. The research was carried out in the farms in the Lviv region, in April-May at the bees of the Carpathian breed. It was established that the content of Ferrum, Cuprum and Germanium in the tissues of bees increases with the addition of soybean flour with Сobalt citrate at a dose of 2 mg per 500 g of soy flour. When Nickel citrate was added to the feed at a dose of 1 mg per 500 g of soy flour, the content of Ferrum and Cuprum increased in honeycombs. The complex combination of Citrits Co and Ni, soy flour with sugar syrup was characterized by a decrease in the level of Zn in the tissues of the bees compared to its contents in the control group samples. In samples of biological material, the content of Сobalt, Nickel and the essential elements Cuprum, Zink, Ferrum, Selenium, Germanium was determined by atomic emission spectrometry with inductively coupled plasma. Citrates of the microelements were produced by the method of M. Kosinov and V. Kaplunenkо.
矿物质营养不足导致蜜蜂体内的生理和代谢反应受到抑制。众所周知，氯化钴被用来激活蜂王的产卵。通过纳米技术制备的柠檬酸钴和柠檬酸镍对蜜蜂的矿物质代谢有纠正作用，对蜜蜂的代谢有影响。众所周知，钴在酶的工作中起着重要作用;合成维生素B12，促进维生素A, E, C的吸收;增加蛋白质代谢，参与造血。镍对造血也有明显的影响，即对血液的形态组成有明显的影响。但其对细胞和亚细胞水平的影响尚不清楚。本文介绍了在大豆粉中添加柠檬酸钴和柠檬酸镍对蜜蜂和蜂巢机体组织中矿质元素含量的影响。这项研究于4月至5月在利沃夫地区的农场进行，研究对象是喀尔巴阡山脉的蜜蜂品种。结果表明，每500克豆粉中添加Сobalt柠檬酸盐2毫克，蜜蜂组织中铁、铜和锗的含量会增加。在饲料中添加柠檬酸镍，每500 g豆粉添加1 mg，可提高蜂窝中铁和铜的含量。与对照组样品相比，柑橘Co和Ni、大豆粉和糖浆的复杂组合的特点是蜜蜂组织中Zn的含量降低。采用电感耦合等离子体原子发射光谱法测定了生物材料样品中Сobalt、镍和必需元素铜、锌、铁、硒、锗的含量。微量元素柠檬酸盐采用M. Kosinov法和V. kaplunenk法制备。
{"title":"Mineral composition of the organism tissues and honeycombs of melliferous bees under the conditions of feeding them soybean flour and citrates of Cobalt and Nickel","authors":"A. Pashchenko, I. Kovalchuk, R. Fedoruk","doi":"10.32718/NVLVET9311","DOIUrl":"https://doi.org/10.32718/NVLVET9311","url":null,"abstract":"The inadequacy of mineral nutrition leads to inhibition of physiological and metabolic reactions in the body of honeybees. It is known that Cobalt chloride is used to activate oviposition of the queen bee. It was established that Cobalt and Nickel citrate, obtained by the method of nanotechnology, corrects the mineral metabolism and affects the metabolism of bees. It is known that Cobalt plays an important role in the work of enzymes; synthesis of vitamin B12, promotes assimilation of vitamins A, E, C; increases protein metabolism, participates in hematopoiesis. Nickel also has a pronounced effect on hemopoiesis, namely on the morphological composition of blood. But its effect on the cell and subcellular level is not well understood. The results of studies of the effect of soybean flour with the addition of Cobalt and Nickel citrates on the content of mineral elements in the tissues of the body of bees and honeycombs are given. The research was carried out in the farms in the Lviv region, in April-May at the bees of the Carpathian breed. It was established that the content of Ferrum, Cuprum and Germanium in the tissues of bees increases with the addition of soybean flour with Сobalt citrate at a dose of 2 mg per 500 g of soy flour. When Nickel citrate was added to the feed at a dose of 1 mg per 500 g of soy flour, the content of Ferrum and Cuprum increased in honeycombs. The complex combination of Citrits Co and Ni, soy flour with sugar syrup was characterized by a decrease in the level of Zn in the tissues of the bees compared to its contents in the control group samples. In samples of biological material, the content of Сobalt, Nickel and the essential elements Cuprum, Zink, Ferrum, Selenium, Germanium was determined by atomic emission spectrometry with inductively coupled plasma. Citrates of the microelements were produced by the method of M. Kosinov and V. Kaplunenkо.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89416375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PCV2-infection of pigs plays an important role in the infectious pathology of pigs, having a significant impact on the economy of pig farms in different countries. Pathomorphological changes in the body of pigs with PCV2-infection occur moderately and in stages, as a result of the simultaneous launch of a set of mechanisms to counter the causative agent of the disease, resulting in the formation of a whole spectrum of reactive and pathological processes. The main “target” for PCV-2 is the immune system, and the multiplication of PCV-2 in the cells of the immune system leads to their death and development of immunodeficiency state. Thymus is the central organ of lymphocytopoiesis and imunopoiesis, in it occurs differentiation of all cellular forms of mature T-lymphocytes in the pre-T-lymphocyte stage, which migrate from the bone marrow to the bloodstream. The growth and development of the thymus, as well as the bone organs, is in direct interaction with the growth and development of the spleen, lymph nodes and is reflected in the structure of the interstitial connective tissue and lymphoid parenchyma. Purpose: determination of pathomorphological changes in the thymus of pigs at different stages of the development of clinically expressed PCV2-infection. Methods. The work was carried out in pig farms of Ukraine, with intensive technology of pig rearing, at the department of normal and pathological anatomy of agricultural animals and the Scientific and Research Center for Biosafety and Environmental Control of the Agroindustrial Complex Resources of Dnipro State Agrarian and Economic University. For pathomorphological studies, animals were selected with clinical signs of active PCV2‑infection (in 1 ml of whole blood of which contained more than 107 copies of the PCV-2 virus equivalent gene) and also piglets with positive optical density of specific antibodies (Ig G and Ig M) in serum blood. Pathomorphological changes were characterized by gradual exhaustion of the lymphoid parenchyma with a sharp decrease in the area of the cortical substance of the particles. Against the background of the degradation of the lymphoid parenchyma of the organ, there is a tendency to increase the relative area of the interstitial connective tissue, as well as the absolute size and number of thymic bodies, has been established.
{"title":"Pathomorphological changes of the thymus of pigs at different stages of development of clinically expressed circovirus infection type ІІ","authors":"V. Evert","doi":"10.32718/NVLVET9320","DOIUrl":"https://doi.org/10.32718/NVLVET9320","url":null,"abstract":"PCV2-infection of pigs plays an important role in the infectious pathology of pigs, having a significant impact on the economy of pig farms in different countries. Pathomorphological changes in the body of pigs with PCV2-infection occur moderately and in stages, as a result of the simultaneous launch of a set of mechanisms to counter the causative agent of the disease, resulting in the formation of a whole spectrum of reactive and pathological processes. The main “target” for PCV-2 is the immune system, and the multiplication of PCV-2 in the cells of the immune system leads to their death and development of immunodeficiency state. Thymus is the central organ of lymphocytopoiesis and imunopoiesis, in it occurs differentiation of all cellular forms of mature T-lymphocytes in the pre-T-lymphocyte stage, which migrate from the bone marrow to the bloodstream. The growth and development of the thymus, as well as the bone organs, is in direct interaction with the growth and development of the spleen, lymph nodes and is reflected in the structure of the interstitial connective tissue and lymphoid parenchyma. Purpose: determination of pathomorphological changes in the thymus of pigs at different stages of the development of clinically expressed PCV2-infection. Methods. The work was carried out in pig farms of Ukraine, with intensive technology of pig rearing, at the department of normal and pathological anatomy of agricultural animals and the Scientific and Research Center for Biosafety and Environmental Control of the Agroindustrial Complex Resources of Dnipro State Agrarian and Economic University. For pathomorphological studies, animals were selected with clinical signs of active PCV2‑infection (in 1 ml of whole blood of which contained more than 107 copies of the PCV-2 virus equivalent gene) and also piglets with positive optical density of specific antibodies (Ig G and Ig M) in serum blood. Pathomorphological changes were characterized by gradual exhaustion of the lymphoid parenchyma with a sharp decrease in the area of the cortical substance of the particles. Against the background of the degradation of the lymphoid parenchyma of the organ, there is a tendency to increase the relative area of the interstitial connective tissue, as well as the absolute size and number of thymic bodies, has been established.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83345003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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