Nowadays, there are many different methods for diagnosing cow’ mammary gland diseases. This is a clinical examination of the general condition of the animal, organoleptic, physico-chemical, bacteriological research of the secretion of the mammary gland, radiological, ultrasound, laser, histological, cytological diagnostics, etc. Since all these methods in most cases are aimed at evaluating functional changes in the mammary gland and do not reflect the structural changes of the organ, so in the development of therapeutic and preventive measures to prevent the occurrence of pathological processes in the mammary gland in cows do not display information on pathomorphological changes in the tissues of mammary glands for mastitis of various forms. Histological diagnostics becomes an integral part of the clinical examination of animals in the activity of the doctor of veterinary medicine, because it allows taking into account the changes occurring in the tissues of the mammary gland, not only on the macroscopic, but also on the microscopic levels, which is especially important in differential diagnosis. Microscopic studies of biopsy revealed that normally the wall of the lacrimal alveolus consists of a single-layer cubic epithelium, a layer of myoepithelial (basintine) cells, a basement membrane and perialveolar connective tissue that contains numerous blood and lymphatic capillaries and nerve fibers. On the internal walls of the alveoli, there are secretory cells (lactoxytes) which, at different stages of their accumulation of secretion, or its separation into the lumen of the alveoli. The entire lumen of the alveoli is completely filled with a secret, which is rather intensely colored with eosin. In damaged alveoli, we detected the destruction of in situ lacticates, their excretion into the lumen with subsequent destruction there. In the destruction and removal of a small number of lacto-cysts in the lumen of the alveoli was a formed secret, in which an increased number of lactocytes and single neutrophils and lymphocytes were recorded. The given results indicate the pathohistological changes in cows’ mammary gland tissues under the chronic form of the mastitis. The method can be used as one of the important tests in the differential diagnosis of mastitis, which makes it possible to carry out in-depth study of the structure of the mammary gland of cows for various types of pathology using the method of biopsy of tissues from the affected area and the histological examination of the biopsy. Histology, as the most important scientific instrument of biology and medicine, is the only way to detect pathological changes in the tissues of the mammary gland. That is why histology of breast pathology serves as the most accurate diagnostic method in veterinary medicine and helps to choose the most effective way of treatment.
{"title":"Morphological changes in cows’ mammary gland tissue under chronic form of mastitis","authors":"A. Mazurkevych, A. Hryshchuk, I. Hryshchuk","doi":"10.32718/NVLVET9319","DOIUrl":"https://doi.org/10.32718/NVLVET9319","url":null,"abstract":"Nowadays, there are many different methods for diagnosing cow’ mammary gland diseases. This is a clinical examination of the general condition of the animal, organoleptic, physico-chemical, bacteriological research of the secretion of the mammary gland, radiological, ultrasound, laser, histological, cytological diagnostics, etc. Since all these methods in most cases are aimed at evaluating functional changes in the mammary gland and do not reflect the structural changes of the organ, so in the development of therapeutic and preventive measures to prevent the occurrence of pathological processes in the mammary gland in cows do not display information on pathomorphological changes in the tissues of mammary glands for mastitis of various forms. Histological diagnostics becomes an integral part of the clinical examination of animals in the activity of the doctor of veterinary medicine, because it allows taking into account the changes occurring in the tissues of the mammary gland, not only on the macroscopic, but also on the microscopic levels, which is especially important in differential diagnosis. Microscopic studies of biopsy revealed that normally the wall of the lacrimal alveolus consists of a single-layer cubic epithelium, a layer of myoepithelial (basintine) cells, a basement membrane and perialveolar connective tissue that contains numerous blood and lymphatic capillaries and nerve fibers. On the internal walls of the alveoli, there are secretory cells (lactoxytes) which, at different stages of their accumulation of secretion, or its separation into the lumen of the alveoli. The entire lumen of the alveoli is completely filled with a secret, which is rather intensely colored with eosin. In damaged alveoli, we detected the destruction of in situ lacticates, their excretion into the lumen with subsequent destruction there. In the destruction and removal of a small number of lacto-cysts in the lumen of the alveoli was a formed secret, in which an increased number of lactocytes and single neutrophils and lymphocytes were recorded. The given results indicate the pathohistological changes in cows’ mammary gland tissues under the chronic form of the mastitis. The method can be used as one of the important tests in the differential diagnosis of mastitis, which makes it possible to carry out in-depth study of the structure of the mammary gland of cows for various types of pathology using the method of biopsy of tissues from the affected area and the histological examination of the biopsy. Histology, as the most important scientific instrument of biology and medicine, is the only way to detect pathological changes in the tissues of the mammary gland. That is why histology of breast pathology serves as the most accurate diagnostic method in veterinary medicine and helps to choose the most effective way of treatment.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87383816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kalynovskyi, L. Yevtukh, V. Shnaider, V. Zakharin, V. Karpiuk, M. Omelianenko
The paper explores placental barrier permeability to Cadmium (Cd) and Plumbum (Pb) in cows in the dynamics of their pregnancy in the direction ‘maternal blood (MB) → maternal placental compartment (MPC) → fetal placental compartment (FPC) → the umbilical cord (UC) → fetal liver (FL) → amniotic (AMF) and allantoic (ALF) fluid’ and during parturition in relatively clean and contaminated with radioactive nuclides environments, with the injection of tissue medication fetoplacentat in the dry off time, and feeding with a mixture of saponite and sulphur supplementation in rations; in mares at the foaling time in the direction ‘maternal blood (MBm) → the fetal placental compartment of a mare (FPCm) → the umbilical cord of a foal (UCf ) → the foal’s liver (FLf) → amniotic (AMFf) and allantoic (ALFf) fluid → the allantoic membrane of a foal (ALMf)’. The age of the fetus during pregnancy was established by anatomical parameters, whereas Pb and Cd levels in blood and substrates were measured by the method of atomic absorption spectrophotometry (GOST 30170896). The MPC (caruncle) and FPC (cotyledon) were analyzed separately. Their barrier function in relation to Pb and Cd was performed at its full extent in 4–5 months of pregnancy and coincided with the highest concentration in the fetal liver. Wharton’s jelly absorbed Cd in 3–4 months of gestation. With the approach of calving, Pb and Cd levels decreased in the amniotic fluid and increased in the allantoic fluid. Under the influence of fetoplacentat in MPC and FPC Cd accumulated better in AMF and ALF, whereas its levels were lower in the cortical mucus of the cervix. Pb deposited in equal concentrations in MPC of all the cows (0.46 ± 0.18 μg/kg in the contaminated environment and 0.47 ± 0.17 μg/kg in the relatively clean environment). In the relatively clean environment its levels in ALF were lower in 8 times (0.62 ± 0.16 μg/kg and 0.08 ± 0.04 μg/kg) and 26 times lower in AMF (0.52 ± 0.07 μg/kg and 0.02 ± 0.002 μg/kg), whereas Pb concentration in the cortical mucus of the cervix was 17 times lower (0.06 ± 0.03μg/kg) than that in the contaminated environment, (1.01 ± 0.28 μg/kg). Pb transited from MPC (0.46 ± 0.18 μg/kg) to FPC (0.51 ± 0.19 μg/kg), deposited in the internal environment of the uterus wherefrom it was absorbed by the cortical mucus of the cervix (1.01 ± 0.28 μg/kg). While transiting through the fetal body Pb accumulated in amniotic (0.52 ± 0.07 μg/kg) and allantoic fluid (0.62 ± 0.16 μg/kg). Mineral supplement in rations led to a weakened barrier function of MPC (0.23 ± 0.14 μg/kg), a two-fold increase of Pb in FPC (0.47 ± 0.11 μg/kg), whereas in the allantoic (0.16 ± 0.05 μg/kg) and amniotic (0.38 ± 0.10 μg/kg) fluid Pb concentration was lower when compared to the control group (0.62 ± 0.16 μg/kg; Р < 0.05 і 0.52 ± 0.07 μg/kg; Р < 0.05, respectively). Pb deposited in the cortical mucus of the cervix (2.47 ± 0.26 μg/kg; Р < 0.001). With the injection of fetoplacentat the barrier function of MP
{"title":"Placental barrier permeability to Cadmium and Plumbum during cow pregnancy and at the foaling time of mares","authors":"H. Kalynovskyi, L. Yevtukh, V. Shnaider, V. Zakharin, V. Karpiuk, M. Omelianenko","doi":"10.32718/NVLVET9314","DOIUrl":"https://doi.org/10.32718/NVLVET9314","url":null,"abstract":"The paper explores placental barrier permeability to Cadmium (Cd) and Plumbum (Pb) in cows in the dynamics of their pregnancy in the direction ‘maternal blood (MB) → maternal placental compartment (MPC) → fetal placental compartment (FPC) → the umbilical cord (UC) → fetal liver (FL) → amniotic (AMF) and allantoic (ALF) fluid’ and during parturition in relatively clean and contaminated with radioactive nuclides environments, with the injection of tissue medication fetoplacentat in the dry off time, and feeding with a mixture of saponite and sulphur supplementation in rations; in mares at the foaling time in the direction ‘maternal blood (MBm) → the fetal placental compartment of a mare (FPCm) → the umbilical cord of a foal (UCf ) → the foal’s liver (FLf) → amniotic (AMFf) and allantoic (ALFf) fluid → the allantoic membrane of a foal (ALMf)’. The age of the fetus during pregnancy was established by anatomical parameters, whereas Pb and Cd levels in blood and substrates were measured by the method of atomic absorption spectrophotometry (GOST 30170896). The MPC (caruncle) and FPC (cotyledon) were analyzed separately. Their barrier function in relation to Pb and Cd was performed at its full extent in 4–5 months of pregnancy and coincided with the highest concentration in the fetal liver. Wharton’s jelly absorbed Cd in 3–4 months of gestation. With the approach of calving, Pb and Cd levels decreased in the amniotic fluid and increased in the allantoic fluid. Under the influence of fetoplacentat in MPC and FPC Cd accumulated better in AMF and ALF, whereas its levels were lower in the cortical mucus of the cervix. Pb deposited in equal concentrations in MPC of all the cows (0.46 ± 0.18 μg/kg in the contaminated environment and 0.47 ± 0.17 μg/kg in the relatively clean environment). In the relatively clean environment its levels in ALF were lower in 8 times (0.62 ± 0.16 μg/kg and 0.08 ± 0.04 μg/kg) and 26 times lower in AMF (0.52 ± 0.07 μg/kg and 0.02 ± 0.002 μg/kg), whereas Pb concentration in the cortical mucus of the cervix was 17 times lower (0.06 ± 0.03μg/kg) than that in the contaminated environment, (1.01 ± 0.28 μg/kg). Pb transited from MPC (0.46 ± 0.18 μg/kg) to FPC (0.51 ± 0.19 μg/kg), deposited in the internal environment of the uterus wherefrom it was absorbed by the cortical mucus of the cervix (1.01 ± 0.28 μg/kg). While transiting through the fetal body Pb accumulated in amniotic (0.52 ± 0.07 μg/kg) and allantoic fluid (0.62 ± 0.16 μg/kg). Mineral supplement in rations led to a weakened barrier function of MPC (0.23 ± 0.14 μg/kg), a two-fold increase of Pb in FPC (0.47 ± 0.11 μg/kg), whereas in the allantoic (0.16 ± 0.05 μg/kg) and amniotic (0.38 ± 0.10 μg/kg) fluid Pb concentration was lower when compared to the control group (0.62 ± 0.16 μg/kg; Р < 0.05 і 0.52 ± 0.07 μg/kg; Р < 0.05, respectively). Pb deposited in the cortical mucus of the cervix (2.47 ± 0.26 μg/kg; Р < 0.001). With the injection of fetoplacentat the barrier function of MP","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89937213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-02DOI: 10.32718/10.32718/NVLVET9308
V. Yevstafieva, V. Yeresko
Many years of experience in fighting and preventing of helminthiasis in waterfowl shows that the successful elimination of parasitic diseases is possible under the conditions of a complex of organizational and economic, veterinary and sanitary and special anti-parasitic measures, which must necessarily include deworming of poultry. The purpose of the work was to study the effectiveness of modern antihelminthic drugs for the spontaneous capillariasis geese. Experimental testing of antihelminthic preparations of domestic production was performed: piperazine 45% (DR – piperazine adipinat), 22% phenzole (DR – fenbendazole) and brovadazole plus (DR – piperazine adipinat, phenbendazole). The efficacy of the drugs was determined by the results of helminthic autopsy and coproscopicof the geese of the experimental and control groups. The main indicators of the action of drugs were extensiveness and intensive. It was established that according to the results of helminthocoproscopic investigations, the most effective drugs for capillariasis geese were phenzole 22% and brovadazole plus, their effectiveness at day 15 of the experiment was 100.0%. Efficiency (EE and IE) of piperazine 45% was slightly lower and at day 5 of treatment, according to the results of the coproscopy, was 40.0% and 65.0% respectively, for 10 days – 70.0% and 76.0%, for 15 days – 70% , 0 and 82.0%. At the same time, according to the results of helminthic autopsy geese in the process of their treatment, the effectiveness of the preparations of phenzole 22% and piperazine 45% were lower. EI of experimental geese for 15 days with the use of phenzole 22% was 40.0%, and II decreased from 21.20 ± 1.16 to 2.00 ± 1.00 ex./head. In the application of piperazine, 45% of the experimental poultry EI for 15 days reached 60.0%, II decreased from 22.40 ± 1.12 to 3.67 ± 0.58 ex./head. Extens- and intensefficiency of piperazine 45% was 40.0 and 86.0%, phenzole 22% – 60.0 and 92.0%, respectively. It was determined that the most effective preparation for geese capillariasis is brovadazole plus (EE, IE – 100.0%), which is confirmed by the results of coproscopic studies and helminthic autopsy. Based on the data obtained, confirmed by experimental studies, we recommend the use of the drug brovadazol plus in the control and prevention of capillariasis geese.
{"title":"Therapeutic effectiveness of modern antihelminthic drugs for geese capillariasis","authors":"V. Yevstafieva, V. Yeresko","doi":"10.32718/10.32718/NVLVET9308","DOIUrl":"https://doi.org/10.32718/10.32718/NVLVET9308","url":null,"abstract":"Many years of experience in fighting and preventing of helminthiasis in waterfowl shows that the successful elimination of parasitic diseases is possible under the conditions of a complex of organizational and economic, veterinary and sanitary and special anti-parasitic measures, which must necessarily include deworming of poultry. The purpose of the work was to study the effectiveness of modern antihelminthic drugs for the spontaneous capillariasis geese. Experimental testing of antihelminthic preparations of domestic production was performed: piperazine 45% (DR – piperazine adipinat), 22% phenzole (DR – fenbendazole) and brovadazole plus (DR – piperazine adipinat, phenbendazole). The efficacy of the drugs was determined by the results of helminthic autopsy and coproscopicof the geese of the experimental and control groups. The main indicators of the action of drugs were extensiveness and intensive. It was established that according to the results of helminthocoproscopic investigations, the most effective drugs for capillariasis geese were phenzole 22% and brovadazole plus, their effectiveness at day 15 of the experiment was 100.0%. Efficiency (EE and IE) of piperazine 45% was slightly lower and at day 5 of treatment, according to the results of the coproscopy, was 40.0% and 65.0% respectively, for 10 days – 70.0% and 76.0%, for 15 days – 70% , 0 and 82.0%. At the same time, according to the results of helminthic autopsy geese in the process of their treatment, the effectiveness of the preparations of phenzole 22% and piperazine 45% were lower. EI of experimental geese for 15 days with the use of phenzole 22% was 40.0%, and II decreased from 21.20 ± 1.16 to 2.00 ± 1.00 ex./head. In the application of piperazine, 45% of the experimental poultry EI for 15 days reached 60.0%, II decreased from 22.40 ± 1.12 to 3.67 ± 0.58 ex./head. Extens- and intensefficiency of piperazine 45% was 40.0 and 86.0%, phenzole 22% – 60.0 and 92.0%, respectively. It was determined that the most effective preparation for geese capillariasis is brovadazole plus (EE, IE – 100.0%), which is confirmed by the results of coproscopic studies and helminthic autopsy. Based on the data obtained, confirmed by experimental studies, we recommend the use of the drug brovadazol plus in the control and prevention of capillariasis geese.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"163 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86432156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recently, a significant amount of data has been reported indicating on the link between consumption of dairy products and development of oncological diseases. This could be explained by the presence in milk of various hormones, particularly insulin-like growth factor (IGF). This hormone is a mediator between somatotropin and tissues. In response to the increase of the blood level of growth hormone, hepatocytes begin actively synthesize the IGF, which accelerates the rate of the metabolism, including those of onco-cells. The majority of studies relate to humane medicine, and data on the dependence of IGF milk levels on breed, period of lactation, diet, physiological, and clinical status of an animal are lacking. Taking this into consideration, the purpose of this study was to analyze the concentration of insulin-like factor in colostrum, milk of cows and blood plasma at various stages of lactation. Study was performed on cows of Ukrainian black-and-white dairy breed in their from second to fifth lactation with milk yield 5100–5700 kg per previous lactation. Concentration of IGF was detected in colostrum, milk and plasma of cows, using enzyme immunoassay. Obtained material gives evidence that the highest IGF level is present in colostrum. After that hormone concentration decreases up to 10–14 day of lactation. High level of IGF in colostrum might be associated with necessity to stimulate metabolism of calves in early postnatal period of ontogenesis. Immediately after calving plasma level of IGF decreases. The highest level was observed on 10–14th days of lactation. Taking into consideration physiological function of the given hormone, there is no univocal explanation of the decrease of its plasma level in cows at the beginning of lactation. This could be associated with energy deficit, physiological condition of animals or with developing of lactation dominant and levels of other hormones. Potential of further research lies in investigation of the dependence of the IGF level in the secretion of the mammary gland on productivity, breed, age and diet of animals.
{"title":"The concentration of insuline-like growth factor in colostrum, milk and plasma of cows","authors":"D. V. Hrudetska, M. Simonov, O. Dashkovskyy","doi":"10.32718/NVLVET9321","DOIUrl":"https://doi.org/10.32718/NVLVET9321","url":null,"abstract":"Recently, a significant amount of data has been reported indicating on the link between consumption of dairy products and development of oncological diseases. This could be explained by the presence in milk of various hormones, particularly insulin-like growth factor (IGF). This hormone is a mediator between somatotropin and tissues. In response to the increase of the blood level of growth hormone, hepatocytes begin actively synthesize the IGF, which accelerates the rate of the metabolism, including those of onco-cells. The majority of studies relate to humane medicine, and data on the dependence of IGF milk levels on breed, period of lactation, diet, physiological, and clinical status of an animal are lacking. Taking this into consideration, the purpose of this study was to analyze the concentration of insulin-like factor in colostrum, milk of cows and blood plasma at various stages of lactation. Study was performed on cows of Ukrainian black-and-white dairy breed in their from second to fifth lactation with milk yield 5100–5700 kg per previous lactation. Concentration of IGF was detected in colostrum, milk and plasma of cows, using enzyme immunoassay. Obtained material gives evidence that the highest IGF level is present in colostrum. After that hormone concentration decreases up to 10–14 day of lactation. High level of IGF in colostrum might be associated with necessity to stimulate metabolism of calves in early postnatal period of ontogenesis. Immediately after calving plasma level of IGF decreases. The highest level was observed on 10–14th days of lactation. Taking into consideration physiological function of the given hormone, there is no univocal explanation of the decrease of its plasma level in cows at the beginning of lactation. This could be associated with energy deficit, physiological condition of animals or with developing of lactation dominant and levels of other hormones. Potential of further research lies in investigation of the dependence of the IGF level in the secretion of the mammary gland on productivity, breed, age and diet of animals.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72735698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Protein metabolism in animals is perhaps the most informative indicator of anthropogenic environmental pollution, because it provides an opportunity to characterize metabolic processes in the body. They are of the utmost importance for the structure and function of all cells, tissues and organs. Proteins reflect the state of the organism, as well as those changes that occur in it under the influence of internal and external factors. We studied the dynamics of changes in indicators in protein metabolism in cows depending on the season of the year and the location of a farm. The research was carried out in two farms of the Lviv region: the Danylo Halytskyj LLC, near the State Mining and Chemical Enterprise “Sirka”, and FE “Lelyk” of Zhovkva district, in spring, summer, autumn and winter periods of keeping. The object of research was the blood of 20 cows of Ukrainian Black-Spotted breed. According to the results of the research, a reduction in total protein, relative to the physiological norm of 0.45 g/l. in spring, and by 0.64 g/l in the summer, dysproteinemia, a high level of gamma globulin fraction (50.30%) in cows from the Danylo Halytskyj farm, located in the zone of influence of the State Chemical Medicine Plant “Sirka”. In animals FE Lelyk, located in the conventionally clean zone, this indicator was significantly higher, and was 76.14 g/l in spring, and 78.17 g/l in summer, and also in animals of this farm dysproteinemia was not observed. In the autumn, at the end of the summer grazing period of the keeping, the index of total protein tended to decrease in the animals of the Danylo Halytsky's Ltd., and in winter it was normalized and invested in the limits of the physiological norm. Albumin fraction of protein in this group of animals, throughout the search period, had a tendency to increase. In the cows of the FE “Lelyk”, the indices of protein metabolism were within the limits of the physiological norm in all periods of research, with the inherent fluctuations in the season of the year. The obtained results due to the content of total protein and protein fractions in the plasma of blood of cows kept in territories with different ecological status indicate that the effect of the activity of the State Chemical Medicine Plant “Sirka” is sufficiently strong on the organism of animals.
{"title":"Dynamics of changes in protein metabolism rates in cows depending on the season of the year and the location of the farm","authors":"U. Vus, O. Kozenko","doi":"10.32718/NVLVET9329","DOIUrl":"https://doi.org/10.32718/NVLVET9329","url":null,"abstract":"Protein metabolism in animals is perhaps the most informative indicator of anthropogenic environmental pollution, because it provides an opportunity to characterize metabolic processes in the body. They are of the utmost importance for the structure and function of all cells, tissues and organs. Proteins reflect the state of the organism, as well as those changes that occur in it under the influence of internal and external factors. We studied the dynamics of changes in indicators in protein metabolism in cows depending on the season of the year and the location of a farm. The research was carried out in two farms of the Lviv region: the Danylo Halytskyj LLC, near the State Mining and Chemical Enterprise “Sirka”, and FE “Lelyk” of Zhovkva district, in spring, summer, autumn and winter periods of keeping. The object of research was the blood of 20 cows of Ukrainian Black-Spotted breed. According to the results of the research, a reduction in total protein, relative to the physiological norm of 0.45 g/l. in spring, and by 0.64 g/l in the summer, dysproteinemia, a high level of gamma globulin fraction (50.30%) in cows from the Danylo Halytskyj farm, located in the zone of influence of the State Chemical Medicine Plant “Sirka”. In animals FE Lelyk, located in the conventionally clean zone, this indicator was significantly higher, and was 76.14 g/l in spring, and 78.17 g/l in summer, and also in animals of this farm dysproteinemia was not observed. In the autumn, at the end of the summer grazing period of the keeping, the index of total protein tended to decrease in the animals of the Danylo Halytsky's Ltd., and in winter it was normalized and invested in the limits of the physiological norm. Albumin fraction of protein in this group of animals, throughout the search period, had a tendency to increase. In the cows of the FE “Lelyk”, the indices of protein metabolism were within the limits of the physiological norm in all periods of research, with the inherent fluctuations in the season of the year. The obtained results due to the content of total protein and protein fractions in the plasma of blood of cows kept in territories with different ecological status indicate that the effect of the activity of the State Chemical Medicine Plant “Sirka” is sufficiently strong on the organism of animals.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73699274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Khimich, O. Piven, O. M. Gorobey, V. Salata, D. V. Freiuk, O. V. Naidich
The main direction of state policy regarding the guaranty safety and quality of animal products is the creation of conditions that are safe for human health during the production and sale of food raw materials. According to current legislation of Ukraine responsibility for the safety of food products placed on the manufacturer but government control plays an important role. This is especially true today, when almost 69.5% of meat is produced in private home farms. It is mainly sold in the agro-industrial markets. The State Service of Ukraine for Food Safety should perform the conditions of modern European regulation and base its activities on the basis of risk assessment. One of the risks are parasitosis, as their negative effect affects the health of millions of people in the world. In this cjnnection the purpose of the study was to analyze the dynamics of the identification of invasive diseases at the stages of slaughter of animals and the sale of slaughter products. Research material was reporting on veterinary medicine (Form № 5-vet and Form № 6-vet) of a number of districts of Odessa region for 2014‒2017. It was founded that 75943 animals were slaughtered in 2014‒2017: 91.18% pigs, 8.14% cattle and 0.68% sheep. The analysis of the dynamics of slaughter, depending on its location, showed that from 41.85% to 99.81% of animals were slaughtered at courtyard. According to the results of veterinary-sanitary control and supervision during the slaughter for the studied years there were no cases of diseases. It was founded that 22617 animal’s carcasses came to the agrofood markets of the regions for the years 2014‒2017, among which 89.56% were pig carcasses, 10,11% cattle carcasses and only 0.33% sheep. According to results of veterinary-sanitary examination in the conditions of SLVSE in the markets, specialists established 351 cases of diseases. In the study of pig carcasses 246 cases of diseases were detected: among them of non-communicable (45.93%) and invasive (54.07%) etiology (echinococcosis). Luring the expertise of cattle carcases was founded 96 cases of diseases: – dicroceliosis (98.96%) and exinococcosis (1.04%); during the expertise of sheep’s carcases – 9 cases of diseases – dicroceliosis (66.67%) and exinococcosis (33.33%). Considering that during the reporting period, according to the results of veterinary-sanitary control and supervision during the slaughter of animals, no cases of diseases were detected, but at the same time the number of cases of detection of invasive diseases during veterinary-sanitary examination in SLVSE on markets constantly increases risk management at the stage of slaughter can be considered not sufficiently effective. Also, the results of our research indicate that the current system for managing the safety of slaughter products is still based primarily on the study of the final product. It does not take into account all possible risks at the previous stages of production. This system can’t fully guarantee to
{"title":"The analysis of the dynamics of detection animal’s invasive diseases during veterinary expertise","authors":"M. Khimich, O. Piven, O. M. Gorobey, V. Salata, D. V. Freiuk, O. V. Naidich","doi":"10.32718/NVLVET9326","DOIUrl":"https://doi.org/10.32718/NVLVET9326","url":null,"abstract":"The main direction of state policy regarding the guaranty safety and quality of animal products is the creation of conditions that are safe for human health during the production and sale of food raw materials. According to current legislation of Ukraine responsibility for the safety of food products placed on the manufacturer but government control plays an important role. This is especially true today, when almost 69.5% of meat is produced in private home farms. It is mainly sold in the agro-industrial markets. The State Service of Ukraine for Food Safety should perform the conditions of modern European regulation and base its activities on the basis of risk assessment. One of the risks are parasitosis, as their negative effect affects the health of millions of people in the world. In this cjnnection the purpose of the study was to analyze the dynamics of the identification of invasive diseases at the stages of slaughter of animals and the sale of slaughter products. Research material was reporting on veterinary medicine (Form № 5-vet and Form № 6-vet) of a number of districts of Odessa region for 2014‒2017. It was founded that 75943 animals were slaughtered in 2014‒2017: 91.18% pigs, 8.14% cattle and 0.68% sheep. The analysis of the dynamics of slaughter, depending on its location, showed that from 41.85% to 99.81% of animals were slaughtered at courtyard. According to the results of veterinary-sanitary control and supervision during the slaughter for the studied years there were no cases of diseases. It was founded that 22617 animal’s carcasses came to the agrofood markets of the regions for the years 2014‒2017, among which 89.56% were pig carcasses, 10,11% cattle carcasses and only 0.33% sheep. According to results of veterinary-sanitary examination in the conditions of SLVSE in the markets, specialists established 351 cases of diseases. In the study of pig carcasses 246 cases of diseases were detected: among them of non-communicable (45.93%) and invasive (54.07%) etiology (echinococcosis). Luring the expertise of cattle carcases was founded 96 cases of diseases: – dicroceliosis (98.96%) and exinococcosis (1.04%); during the expertise of sheep’s carcases – 9 cases of diseases – dicroceliosis (66.67%) and exinococcosis (33.33%). Considering that during the reporting period, according to the results of veterinary-sanitary control and supervision during the slaughter of animals, no cases of diseases were detected, but at the same time the number of cases of detection of invasive diseases during veterinary-sanitary examination in SLVSE on markets constantly increases risk management at the stage of slaughter can be considered not sufficiently effective. Also, the results of our research indicate that the current system for managing the safety of slaughter products is still based primarily on the study of the final product. It does not take into account all possible risks at the previous stages of production. This system can’t fully guarantee to ","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75849535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article presents the results of the research concerning the morphological changes in the blood of the predatory fish species (perch, pike and zander) сaused by nematodical parasitic disease – eustrongylidosis. The research has been conducted in 2014–2018. It has been found that in the blood of perch with eustrongylidosis the hemoglobin and the red-blood-cell count decreased by 6.87 and 42.11 respectively, the white-blood-cell count increased by 8.95%; the basophils and pseudobasophils count – by 129.17%; the eosinophils and pseudoeosinophils count – by 131%; the number of band and segmented neutrophils increased by 27.92 and 37.5% respectively; the young neutrophils were not registered; the number of monocytes – by 16.34%; the lymphocyte count reduced by 3.27% in contrast with a fish that were not sick with the disease. The significant morphological changes in the blood of pikes with eustrongylidosis were also observed. Thus, the hemoglobin concentration, the number of red blood cells and lymphocytes decreased by 11.02, 26.32 and 3.16% respectively. The total leukocytes count increased by 22.02%; the basophils and pseudobasophils count – by 114%; the eosinophils and pseudoiesinophils count – by 95.83%; the number of band and segmented neutrophils increased by 1.71 and 46.72% respectively; a reduction of the young neutrophil granulocytes amount to 26% was noted; the number of monocytes of the blood increased by 25.83% in contrast with pikes that were not affected by the eustrongylidosis. A reduction of the red-blood-cell and lymphocytes amount, as well as hemoglobin concentration – to 30.05, 4.4 та 6.3% respectively was observed in hematological screening of the blood of zanders. The amount of leukocytes in the blood of infested fish increased by 10.6%; the basophils and pseudobasophils count – by 69.23%; the eosinophils and pseudoeosinophils count – by 116.67%; the number of band and segmented neutrophils increased by 15.58 and 23.27% respectively; the elevation of the number of young neutrophils to 5.75% was registered; the number of monocytes increased by 17.46% in contrast with zanders that were not infested.
本文介绍了捕食鱼类(鲈鱼、梭鱼和梭鱼)因线虫性寄生虫病——全圆线虫病引起的血液形态变化的研究结果。这项研究是在2014-2018年进行的。结果表明，常圆线虫病鲈鱼血液中血红蛋白和红细胞计数分别下降6.87和42.11，白细胞计数上升8.95%;嗜碱性粒细胞和假嗜碱性粒细胞减少129.17%;嗜酸性粒细胞和假嗜酸性粒细胞减少131%;带状和节段中性粒细胞数量分别增加27.92%和37.5%;年轻的中性粒细胞未被登记;单核细胞数量-减少16.34%;与未患病的鱼相比，淋巴细胞计数减少了3.27%。常圆圆线虫病的猪血中也观察到明显的形态学变化。血红蛋白浓度、红细胞和淋巴细胞数量分别下降11.2%、26.32%和3.16%。白细胞总数增加22.02%;嗜碱性粒细胞和假嗜碱性粒细胞减少114%;嗜酸性粒细胞和假嗜酸性粒细胞占95.83%;带状和分节中性粒细胞数量分别增加1.71%和46.72%;年轻中性粒细胞减少26%;与未受正圆线虫病影响的猪相比，血中单核细胞数量增加了25.83%。zanders血液血液学筛查中观察到红细胞和淋巴细胞数量以及血红蛋白浓度-分别降低至30.05,4.4 та 6.3%。侵染鱼血液中白细胞数量增加10.6%;嗜碱性粒细胞和假嗜碱性粒细胞占69.23%;嗜酸性粒细胞和假嗜酸性粒细胞减少116.67%;带状和节段中性粒细胞数量分别增加15.58%和23.27%;年轻中性粒细胞数量上升至5.75%;单核细胞数量较未侵染者增加17.46%。
{"title":"The morphological changes in the blood of the predatory fish species with eustrongylidosis","authors":"S. Goncharov","doi":"10.32718/NVLVET9303","DOIUrl":"https://doi.org/10.32718/NVLVET9303","url":null,"abstract":"The article presents the results of the research concerning the morphological changes in the blood of the predatory fish species (perch, pike and zander) сaused by nematodical parasitic disease – eustrongylidosis. The research has been conducted in 2014–2018. It has been found that in the blood of perch with eustrongylidosis the hemoglobin and the red-blood-cell count decreased by 6.87 and 42.11 respectively, the white-blood-cell count increased by 8.95%; the basophils and pseudobasophils count – by 129.17%; the eosinophils and pseudoeosinophils count – by 131%; the number of band and segmented neutrophils increased by 27.92 and 37.5% respectively; the young neutrophils were not registered; the number of monocytes – by 16.34%; the lymphocyte count reduced by 3.27% in contrast with a fish that were not sick with the disease. The significant morphological changes in the blood of pikes with eustrongylidosis were also observed. Thus, the hemoglobin concentration, the number of red blood cells and lymphocytes decreased by 11.02, 26.32 and 3.16% respectively. The total leukocytes count increased by 22.02%; the basophils and pseudobasophils count – by 114%; the eosinophils and pseudoiesinophils count – by 95.83%; the number of band and segmented neutrophils increased by 1.71 and 46.72% respectively; a reduction of the young neutrophil granulocytes amount to 26% was noted; the number of monocytes of the blood increased by 25.83% in contrast with pikes that were not affected by the eustrongylidosis. A reduction of the red-blood-cell and lymphocytes amount, as well as hemoglobin concentration – to 30.05, 4.4 та 6.3% respectively was observed in hematological screening of the blood of zanders. The amount of leukocytes in the blood of infested fish increased by 10.6%; the basophils and pseudobasophils count – by 69.23%; the eosinophils and pseudoeosinophils count – by 116.67%; the number of band and segmented neutrophils increased by 15.58 and 23.27% respectively; the elevation of the number of young neutrophils to 5.75% was registered; the number of monocytes increased by 17.46% in contrast with zanders that were not infested.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73875208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The results of the veterinary and sanitary examination of food products are based on data from Zhytomyr Regional State Laboratory of the State Service for Safety of Food and Consumer Protection and State Laboratories of Veterinary and Sanitary Expertise of Economic Markets in Zhytomyr and Zhytomyr Oblast for 2018. The main cause of detoxification of offal was invasive disease. Total sales: 80.26325 tons of products and 10397 positive results were obtained, out of which 92 carcasses, 4074 cases of invasive and non-communicable diseases – 10.262 tons, 6231 cases of other food products with a total weight of 70.00125. The analysis of the reporting documentation of the ZHDLDPPS and the state laboratories of all economic markets in Zhytomyr and Zhytomyr region showed that the main causes of milk and dairy products were: mechanical and bacterial contamination, falsification, subclinical mastics, inconsistency of organoleptic parameters with regulatory requirements, fat content and acidity, violation of terms of implementation. Screening test systems (Charm test Chloramphenicol, 4 sensors (antibiotics of Beta-lactam groups, Tetracyclines, Streptomycins, Chloramphenicol) 247 samples of raw milk were checked, of which 2.4% were positive. Security guarantor dairy products in Ukraine remains a system for monitoring sanitary-dangerous pathogens and residual amounts of toxic substances. The pollution of forest fungi and game in the victims of the Chernobyl accident in the Zhytomyr region remains at a rather high level and they are the main source of danger to consumers. The most contaminated samples of fresh mushrooms, whose specific activity exceeded the regulatory requirements of DR-2006 for 137Cs content in 2018, were from Ovruch (5 samples 509.1 – 3375 Bq/kg), Native (1 sample – 1956 Bq/kg) and Zhitnaya Market of Zhytomyr (3 samples 2328–2345 bq/kg at a rate of 500 Bq/kg). Accordingly, samples of dry mushrooms at a norm of 2500 Bq/kg did not meet the normative requirements of Ovruch 1 (6329 Bq/kg), of the Nurses 2 samples (4330 Bq/kg and 4987 Bq/kg) and Olevsk 1 sample (2814Bk/kg. DLVSE the study of 28 samples of dry fungi showed that the specific activity of 50% of samples from the Ovruch region was 2520–5000 Bq/kg, 5.8% of samples from the Emilchinsky district – 2544–2923 Bq/kg, 7.2% of the samples from the Native -397– 29022 Bq/kg and 4.3% of samples from the Olevsk rayon – 2739–2892 Bq/kg. Of the LRDLDPPS examined, 3 samples of game – 1 sample from Ovruch exceeded the permissible levels by 2.3 times (464 Bq/kg), with Lugin in 3.3 times (634 Bq/kg), from the Native – 7.6 times (1531 Bq/kg) .And according to the DLVSE, the level of contamination of the game with 6 samples in the Germans – 4 samples (2000-12124 Bq/kg) In Samara, 1 sample in Korosten amounted to 1,500 Bq kg, of which 48 samples of milk tested by the DLVSE in the Germans all showed an excess of 137Cs (101-172 Bq/kg ), fish-2 samples – 203 and 590 Bq/kg.
{"title":"Actual problems of quality and safety of food products in the context of providing food security in the Zhytomyr region","authors":"V. A. Kotelevych","doi":"10.32718/NVLVET9327","DOIUrl":"https://doi.org/10.32718/NVLVET9327","url":null,"abstract":"The results of the veterinary and sanitary examination of food products are based on data from Zhytomyr Regional State Laboratory of the State Service for Safety of Food and Consumer Protection and State Laboratories of Veterinary and Sanitary Expertise of Economic Markets in Zhytomyr and Zhytomyr Oblast for 2018. The main cause of detoxification of offal was invasive disease. Total sales: 80.26325 tons of products and 10397 positive results were obtained, out of which 92 carcasses, 4074 cases of invasive and non-communicable diseases – 10.262 tons, 6231 cases of other food products with a total weight of 70.00125. The analysis of the reporting documentation of the ZHDLDPPS and the state laboratories of all economic markets in Zhytomyr and Zhytomyr region showed that the main causes of milk and dairy products were: mechanical and bacterial contamination, falsification, subclinical mastics, inconsistency of organoleptic parameters with regulatory requirements, fat content and acidity, violation of terms of implementation. Screening test systems (Charm test Chloramphenicol, 4 sensors (antibiotics of Beta-lactam groups, Tetracyclines, Streptomycins, Chloramphenicol) 247 samples of raw milk were checked, of which 2.4% were positive. Security guarantor dairy products in Ukraine remains a system for monitoring sanitary-dangerous pathogens and residual amounts of toxic substances. The pollution of forest fungi and game in the victims of the Chernobyl accident in the Zhytomyr region remains at a rather high level and they are the main source of danger to consumers. The most contaminated samples of fresh mushrooms, whose specific activity exceeded the regulatory requirements of DR-2006 for 137Cs content in 2018, were from Ovruch (5 samples 509.1 – 3375 Bq/kg), Native (1 sample – 1956 Bq/kg) and Zhitnaya Market of Zhytomyr (3 samples 2328–2345 bq/kg at a rate of 500 Bq/kg). Accordingly, samples of dry mushrooms at a norm of 2500 Bq/kg did not meet the normative requirements of Ovruch 1 (6329 Bq/kg), of the Nurses 2 samples (4330 Bq/kg and 4987 Bq/kg) and Olevsk 1 sample (2814Bk/kg. DLVSE the study of 28 samples of dry fungi showed that the specific activity of 50% of samples from the Ovruch region was 2520–5000 Bq/kg, 5.8% of samples from the Emilchinsky district – 2544–2923 Bq/kg, 7.2% of the samples from the Native -397– 29022 Bq/kg and 4.3% of samples from the Olevsk rayon – 2739–2892 Bq/kg. Of the LRDLDPPS examined, 3 samples of game – 1 sample from Ovruch exceeded the permissible levels by 2.3 times (464 Bq/kg), with Lugin in 3.3 times (634 Bq/kg), from the Native – 7.6 times (1531 Bq/kg) .And according to the DLVSE, the level of contamination of the game with 6 samples in the Germans – 4 samples (2000-12124 Bq/kg) In Samara, 1 sample in Korosten amounted to 1,500 Bq kg, of which 48 samples of milk tested by the DLVSE in the Germans all showed an excess of 137Cs (101-172 Bq/kg ), fish-2 samples – 203 and 590 Bq/kg.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85561763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It is necessary to carry out not only the evaluation of sexual activity of breeder boars and the quality of their sperm, but carefully investigate the metabolic status by laboratory methods during the medical examination. It helps to determine the optimal parameters for the implementation of the reproductive potential of the breeder boars. Biochemical methods of research can establish imbalance in metabolism, the nature and localization of cytolytic processes by the degree of enzymes activity in serum. However, in order to detect inflammatory or destructive changes in the connective tissue structures, in particular the musculoskeletal system, the most informative indexes will be biopolymers of the connective tissue – glycoproteins and proteoglycans. The results of a complex examination of clinically healthy breeder boars with different regime of their production use, which includes an extended set of biochemical tests with the determination of glycoprotein, chondroitin sulfate, total glycosaminoglycans and their fractions, are presented in this paper. It was found that the level of such parameters as the total protein and its fractions, urea, aspartate and alanine aminotransferase activity, and the MB fraction of creatine phosphokinase did not have any significant differences. For breeder boars that were used in reproductive events irregularly (group I), there was an indicative higher level of creatinine and glycosaminoglycans. In animals with regular use as the herd (group II), the level of creatinine and fractions of glycosaminoglycans (GAG) was lower, but there was an increasing in cholesterol, β-lipoproteins, and activity of total creatine phosphokinase. The probable difference in these indicators can be explained by the difference in the intensity of the energy metabolism processes in muscle tissue. Indeed, during long-term hard work of skeletal muscles, not only glycogen stores are used, but also the use of lipids is of great importance. In addition, the raising of cholesterol and β-lipoproteins may be due to their active involvement in the synthesis of steroid hormones. Increasing the activity of total creatine phosphokinase in blood serum of animals with the intensive use in technology probably is the evidence of severe physical activity and the development of destructive processes in muscle tissue (hyperenzymemia is detected in early stages of myopathy). The absence of the most inflammatory processes was evidenced by the unchanged level of serum glycoproteins, which are known as the acute phase tests. Differences in the parameters of the state of connective tissue were determined at the levels of general GAG, their I and III fractions (chondroitin-6-sulfate and heparan sulfate, respectively). In the first group of boars, there was a higher level of I fraction of GAG, that indicated a significant load on the joints' cartilages and the gradual development of destructive processes in them. About the III fraction of GAG, an increasing of the
{"title":"Metabolic profile and content of the connective tissues’ biopolymers in the blood serum of service boars at the different regime of reproductive loading","authors":"O. Timoshenko, G. Vikulina, D. Kibkalo, I. Moraru","doi":"10.32718/NVLVET9304","DOIUrl":"https://doi.org/10.32718/NVLVET9304","url":null,"abstract":"It is necessary to carry out not only the evaluation of sexual activity of breeder boars and the quality of their sperm, but carefully investigate the metabolic status by laboratory methods during the medical examination. It helps to determine the optimal parameters for the implementation of the reproductive potential of the breeder boars. Biochemical methods of research can establish imbalance in metabolism, the nature and localization of cytolytic processes by the degree of enzymes activity in serum. However, in order to detect inflammatory or destructive changes in the connective tissue structures, in particular the musculoskeletal system, the most informative indexes will be biopolymers of the connective tissue – glycoproteins and proteoglycans. The results of a complex examination of clinically healthy breeder boars with different regime of their production use, which includes an extended set of biochemical tests with the determination of glycoprotein, chondroitin sulfate, total glycosaminoglycans and their fractions, are presented in this paper. It was found that the level of such parameters as the total protein and its fractions, urea, aspartate and alanine aminotransferase activity, and the MB fraction of creatine phosphokinase did not have any significant differences. For breeder boars that were used in reproductive events irregularly (group I), there was an indicative higher level of creatinine and glycosaminoglycans. In animals with regular use as the herd (group II), the level of creatinine and fractions of glycosaminoglycans (GAG) was lower, but there was an increasing in cholesterol, β-lipoproteins, and activity of total creatine phosphokinase. The probable difference in these indicators can be explained by the difference in the intensity of the energy metabolism processes in muscle tissue. Indeed, during long-term hard work of skeletal muscles, not only glycogen stores are used, but also the use of lipids is of great importance. In addition, the raising of cholesterol and β-lipoproteins may be due to their active involvement in the synthesis of steroid hormones. Increasing the activity of total creatine phosphokinase in blood serum of animals with the intensive use in technology probably is the evidence of severe physical activity and the development of destructive processes in muscle tissue (hyperenzymemia is detected in early stages of myopathy). The absence of the most inflammatory processes was evidenced by the unchanged level of serum glycoproteins, which are known as the acute phase tests. Differences in the parameters of the state of connective tissue were determined at the levels of general GAG, their I and III fractions (chondroitin-6-sulfate and heparan sulfate, respectively). In the first group of boars, there was a higher level of I fraction of GAG, that indicated a significant load on the joints' cartilages and the gradual development of destructive processes in them. About the III fraction of GAG, an increasing of the","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80352403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vector-Borne Diseases are a variety of infectious and invasive diseases of animals and humans, the agents of which extend from one to another susceptible subject with the participation of hemopoiesis (ticks, insects, etc.). For the purpose of treatment of blood parasitic transmissive diseases, domestic and foreign researchers tested a significant number of drugs of different chemical composition. In connection with the insufficient on the domestic market of drugs on the basis of imidokarb dipropionate for the treatment of blood-parasitic diseases in animals, the Scientific-Production-Technical Enterprise “Brovapharma” established and conducted the state registration of the drug “Imcar-120”, which blocks the synthesis of polyamine; it also has significantly less toxicity than dimeters of aceturates. It provides a broad spectrum of antiprotozoal effects on pathogens of pyroplasmiosis of the genus Babesia (Babesia bovis, B. ovis, B. bigemina, B. colchica, B. equi, B. divergens, B. canis, B. caballi, B. gibsonii i Francaiella colchica); Teilerian species (Theileria annulata, T. sergenti, T. mutans, T. orientalis, T. ovis, T. recondita, T. tarandirangiferis); Nuttallia equi and the genus of Anaplasma (Anaplasma marginale, A. ovis, Ehrlichia canis) with their mono- or mixed infestation. Pre-clinical research “Determination of toxicological properties of the drug Imcar-120” was carried out on the basis of vivarium of the Faculty of Veterinary Medicine of Sumy NAU. Study of parameters of acute toxicity of the study drug was performed on 50 clinically healthy white mice in males and females. Before the experiment, the individual weight of the body of animals selected for the experiment was 18–22 g, the age was 8–9 weeks. In the first stage, preliminary experimental studies were conducted to determine the variation of dose limits before the main stage of the studies. At the same time the drug was administered intragastrically in doses: 2500, 3500, 4500, 5500, 6500, 7500 mg/kg. Each dose was given to three animals. After the introduction of the drug for monitoring animals, the experiment was carried out 14 days, the first day – every hour. For the expanded stage of the experiment, four experimental groups (n = 8) of animal analogues were formed, in which the study drug was injected under the same conditions as in the previous stage of the experiment at a rate of 3800, 4300, 4800 and 5300 mg/kg of body weight. In the course of studies to determine the parameters of acute toxicity of the drug Imkar-120 it was determined half-lettable dose of the drug. According to R. Kerber's method, DL50 was 4456.25 mg/kg, therefore according to the classification of GOST 12.1.007-76 the preparation Imkar-120 should be classified as hazard class III by injection into the stomach – substances are moderately dangerous.
{"title":"The determination of acute toxicity parameters of “Imkar-120”","authors":"A. A. Fotina, V. Levytska","doi":"10.32718/NVLVET9302","DOIUrl":"https://doi.org/10.32718/NVLVET9302","url":null,"abstract":"Vector-Borne Diseases are a variety of infectious and invasive diseases of animals and humans, the agents of which extend from one to another susceptible subject with the participation of hemopoiesis (ticks, insects, etc.). For the purpose of treatment of blood parasitic transmissive diseases, domestic and foreign researchers tested a significant number of drugs of different chemical composition. In connection with the insufficient on the domestic market of drugs on the basis of imidokarb dipropionate for the treatment of blood-parasitic diseases in animals, the Scientific-Production-Technical Enterprise “Brovapharma” established and conducted the state registration of the drug “Imcar-120”, which blocks the synthesis of polyamine; it also has significantly less toxicity than dimeters of aceturates. It provides a broad spectrum of antiprotozoal effects on pathogens of pyroplasmiosis of the genus Babesia (Babesia bovis, B. ovis, B. bigemina, B. colchica, B. equi, B. divergens, B. canis, B. caballi, B. gibsonii i Francaiella colchica); Teilerian species (Theileria annulata, T. sergenti, T. mutans, T. orientalis, T. ovis, T. recondita, T. tarandirangiferis); Nuttallia equi and the genus of Anaplasma (Anaplasma marginale, A. ovis, Ehrlichia canis) with their mono- or mixed infestation. Pre-clinical research “Determination of toxicological properties of the drug Imcar-120” was carried out on the basis of vivarium of the Faculty of Veterinary Medicine of Sumy NAU. Study of parameters of acute toxicity of the study drug was performed on 50 clinically healthy white mice in males and females. Before the experiment, the individual weight of the body of animals selected for the experiment was 18–22 g, the age was 8–9 weeks. In the first stage, preliminary experimental studies were conducted to determine the variation of dose limits before the main stage of the studies. At the same time the drug was administered intragastrically in doses: 2500, 3500, 4500, 5500, 6500, 7500 mg/kg. Each dose was given to three animals. After the introduction of the drug for monitoring animals, the experiment was carried out 14 days, the first day – every hour. For the expanded stage of the experiment, four experimental groups (n = 8) of animal analogues were formed, in which the study drug was injected under the same conditions as in the previous stage of the experiment at a rate of 3800, 4300, 4800 and 5300 mg/kg of body weight. In the course of studies to determine the parameters of acute toxicity of the drug Imkar-120 it was determined half-lettable dose of the drug. According to R. Kerber's method, DL50 was 4456.25 mg/kg, therefore according to the classification of GOST 12.1.007-76 the preparation Imkar-120 should be classified as hazard class III by injection into the stomach – substances are moderately dangerous.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86883330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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