Due to high mortality, listeriosis is one of the most common causes of death from illnesses associated with food, taking the second place after salmonellosis. Listeriosis, as a rule, arises as a result of consumption of contaminated products, including meat products, cheese, ready-to-eat foods. L. monocytogenes belongs to the third group of pathogenicity. Contamination by L. monocytogenes in processing of products is a constant problem in food plants. Food contamination Listeria leads to a withdrawal of products that produces economic losses. Analysis of the dynamic detection and of the differential identification of Listeria spp. in the meat products of poultry processing enterprises in Dnipropetrovsk region was conducted. The research was carried out by Dnipropetrovsk regional state laboratory of the state service of Ukraine for food safety and consumer protection. The results of bacteriological researches of meat samples which poultry plants gave for microbiological analysis during period 2008–2018 were used for monitoring. Microbiological research was carried out in accordance with valid international normative documents. The fluorescence analyzer Mini Vidas, France, the CAMP test were used for analysis. The biochemical properties of isolated microorganisms were established using BioMerieux API tests, France. Analyzing the number of researches and identification of microorganisms in the Dnipropetrovsk region for the period of 11 years, 3001 positive results out of 8172 analyzed samples were found (36.7%). Herewith, part of positive samples goes up from 8.5% in 2008 to 77.9% in 2018. L. ivanovii was isolated in 1523 samples (18.6%), L. inocua – 833 (10.2%), L. monocytogenes – 493 (6%), L. seeliger – 97 (1.2%), L. grayi – 36 (0.4%), L. welshimeri in 19 samples of meat products (0.2%) out of the 8172 microbiological studies conducted over 11 years. Of the six types of identified Listeria, more than half were L. ivanovii, which is twice as high as cases with L. incocua and thrice compared to L. monocytogenes.
{"title":"Monitoring of the Listeria spp. identification from the poultry products in the Dnipropetrovsk region","authors":"N. Zazharska, I. Borovuk","doi":"10.32718/NVLVET9318","DOIUrl":"https://doi.org/10.32718/NVLVET9318","url":null,"abstract":"Due to high mortality, listeriosis is one of the most common causes of death from illnesses associated with food, taking the second place after salmonellosis. Listeriosis, as a rule, arises as a result of consumption of contaminated products, including meat products, cheese, ready-to-eat foods. L. monocytogenes belongs to the third group of pathogenicity. Contamination by L. monocytogenes in processing of products is a constant problem in food plants. Food contamination Listeria leads to a withdrawal of products that produces economic losses. Analysis of the dynamic detection and of the differential identification of Listeria spp. in the meat products of poultry processing enterprises in Dnipropetrovsk region was conducted. The research was carried out by Dnipropetrovsk regional state laboratory of the state service of Ukraine for food safety and consumer protection. The results of bacteriological researches of meat samples which poultry plants gave for microbiological analysis during period 2008–2018 were used for monitoring. Microbiological research was carried out in accordance with valid international normative documents. The fluorescence analyzer Mini Vidas, France, the CAMP test were used for analysis. The biochemical properties of isolated microorganisms were established using BioMerieux API tests, France. Analyzing the number of researches and identification of microorganisms in the Dnipropetrovsk region for the period of 11 years, 3001 positive results out of 8172 analyzed samples were found (36.7%). Herewith, part of positive samples goes up from 8.5% in 2008 to 77.9% in 2018. L. ivanovii was isolated in 1523 samples (18.6%), L. inocua – 833 (10.2%), L. monocytogenes – 493 (6%), L. seeliger – 97 (1.2%), L. grayi – 36 (0.4%), L. welshimeri in 19 samples of meat products (0.2%) out of the 8172 microbiological studies conducted over 11 years. Of the six types of identified Listeria, more than half were L. ivanovii, which is twice as high as cases with L. incocua and thrice compared to L. monocytogenes.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80195616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article deals with the analytical methods that have allowed to provide detailed information on the material content of bones and structural organizations of both inorganic and organic bone components. The information provided in the present search may increase the knowledge of the influence of fumonisins on the structure and bone in general. On the basis of osteometric measurements, the geometry of the transverse section of the middle diaphysis is determined. They included a definition both the external and internal diameters of the transverse section of the middle of the diaphysis (both in the medial-lateral, and in the anterior-posterior area). The geometric properties are calculated: cortical cross-sectional area, average relative thickness of walls and cortical index. In addition, since during the force analysis, the bone was loaded in the anterior-posterior area, the vertical cortical index, the moment of inertia of the cross-section and the radius of rotation around the medial-lateral axis were calculated. According to the results of comprehensive studies, we found that the intoxication of animals with fumonisin did not affect the body weight and the weight of the femoral bones, although in the fumonisin-induced doses of rats there was a decrease in the mechanical and geometric properties of the bones. The values of mineral density of bone tissue did not differ between groups, but in the experimental group of animals that were fumonisin intoxicated, the mineral content of bone and the percentage of bone ash were lower than in the control group. A detailed mineral analysis of bones showed that the content of Ca, Cu, Fe, Mn, Sr and Zn in bones was significantly decreased in fumonisin intoxicated rats compared to the control group of animals. In addition, changes in the structure of the bone and mineral phase (reduction in the size of bone hydroxyapatite crystals) were noted. Seventy percent of the bones consist of inorganic mineral hydroxyapatite, which includes calcium phosphate, calcium carbonate, calcium fluoride, calcium hydroxide and citrate. This inorganic component is preferably crystalline, although it may be in amorphous forms. Replacement mechanisms occurring in bone hydroxyapatite include intercrystalline exchange and recrystallization due to the dissolution and reforming of crystals with the addition of new ions to the crystalline structure, which replace the Ca 2+ or adsorbed on the surface of the crystals. The mechanisms of replacement that was marked by changes in the group of animals from intoxicated with fumonisin, changes were noted in the structure of the bone and mineral phase, there was a decrease in the size of bone hydroxyapatite crystals.
{"title":"The determination of mineral density indices of the thigh bone by densitometry data in rats, for experimental fumonizinotoxycosis","authors":"H. Rudyk","doi":"10.32718/NVLVET9330","DOIUrl":"https://doi.org/10.32718/NVLVET9330","url":null,"abstract":"The article deals with the analytical methods that have allowed to provide detailed information on the material content of bones and structural organizations of both inorganic and organic bone components. The information provided in the present search may increase the knowledge of the influence of fumonisins on the structure and bone in general. On the basis of osteometric measurements, the geometry of the transverse section of the middle diaphysis is determined. They included a definition both the external and internal diameters of the transverse section of the middle of the diaphysis (both in the medial-lateral, and in the anterior-posterior area). The geometric properties are calculated: cortical cross-sectional area, average relative thickness of walls and cortical index. In addition, since during the force analysis, the bone was loaded in the anterior-posterior area, the vertical cortical index, the moment of inertia of the cross-section and the radius of rotation around the medial-lateral axis were calculated. According to the results of comprehensive studies, we found that the intoxication of animals with fumonisin did not affect the body weight and the weight of the femoral bones, although in the fumonisin-induced doses of rats there was a decrease in the mechanical and geometric properties of the bones. The values of mineral density of bone tissue did not differ between groups, but in the experimental group of animals that were fumonisin intoxicated, the mineral content of bone and the percentage of bone ash were lower than in the control group. A detailed mineral analysis of bones showed that the content of Ca, Cu, Fe, Mn, Sr and Zn in bones was significantly decreased in fumonisin intoxicated rats compared to the control group of animals. In addition, changes in the structure of the bone and mineral phase (reduction in the size of bone hydroxyapatite crystals) were noted. Seventy percent of the bones consist of inorganic mineral hydroxyapatite, which includes calcium phosphate, calcium carbonate, calcium fluoride, calcium hydroxide and citrate. This inorganic component is preferably crystalline, although it may be in amorphous forms. Replacement mechanisms occurring in bone hydroxyapatite include intercrystalline exchange and recrystallization due to the dissolution and reforming of crystals with the addition of new ions to the crystalline structure, which replace the Ca 2+ or adsorbed on the surface of the crystals. The mechanisms of replacement that was marked by changes in the group of animals from intoxicated with fumonisin, changes were noted in the structure of the bone and mineral phase, there was a decrease in the size of bone hydroxyapatite crystals.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88913337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of the real ways of increasing the production of livestock products is to increase the conservation of young animals, the mortality rate of which remains at this time still very high. Many calf treatment regimens have been developed, but they are not always effective, which prompts scientists and practitioners to improve already known methods of treatment, to find and justify new medical preparation. Material for research were dry-cows, healthy and sick calves. After the clinical examination of cows, the experiment was conducted in two phases: the first phase – in 3 groups of cows, the second – in clinical healthy and sick calves. Mineral supplement of Sapocorm were added to the first experimental group of cows; cows of the second experimental group – the preparation Betamint; animals of control group get basic diet. At the second phase, a group of healthy calves, as well as control and two experimental groups of patients were formed. The first group was treated with Intecol in combination with the probiotic Vetom. Calves of the second group – the preparation Intecoll, but in a complex with electrolyte solution Electrosol oral. The calves of the control group were treated with 4% solution of gentamicin sulfate and 30% solution of natrium thiosulfate. It was found out that during the dry period, cows were often subjected to metabolic disorders, and the causes of digestive disorders in calves were associated with a metabolic disorders in cows as a result of malnutrition. In the first stage of the research, the data obtained show the stimulatory effect of Betamint on the processes of hemopoiesis of the cows. In other side, the probable magnification increase hemoglobin in the blood of cows can be explained by the influence of the components of the preparation on the metabolism of proteins. In cows of the first experimental group, which received the mineral supplement of Sapocorm, there were no probable changes in these indices. The next stage of the work was aimed at studying the effectiveness of preparation for the treatment of sick calves. In the analysis of biochemical parameters, was obtained low effectiveness of control group treatment, which was manifested by a slight increase in the biochemical parameters of the blood of diseased calves, which indicates an ineffective action of preparation. In the treatment of calves in the first group, the general condition was normal within 4–5 days of treatment. This indicates the complex effect of compounds and other elements that are components of the preparation Intecol and probiotic Vetom, on restoring the structure and functions of digestion. An analysis of the biochemical parameters of the blood of the second group when treated with the preparation Intecol and Electrosol oral plagued the normalization of the general state occurred within 2–3 days of treatment. The revealed changes indicate the complex influence of nutrients, which are components of the preparation Electrosol oral. On the basis o
{"title":"State of metabolism of cows at dry period as the basis for reasoning of preven-tion and treatment of calves with gastrointestinal diseases","authors":"V. Honcharenko, G. Gryshchuk, S. Sheremet","doi":"10.32718/NVLVET9310","DOIUrl":"https://doi.org/10.32718/NVLVET9310","url":null,"abstract":"One of the real ways of increasing the production of livestock products is to increase the conservation of young animals, the mortality rate of which remains at this time still very high. Many calf treatment regimens have been developed, but they are not always effective, which prompts scientists and practitioners to improve already known methods of treatment, to find and justify new medical preparation. Material for research were dry-cows, healthy and sick calves. After the clinical examination of cows, the experiment was conducted in two phases: the first phase – in 3 groups of cows, the second – in clinical healthy and sick calves. Mineral supplement of Sapocorm were added to the first experimental group of cows; cows of the second experimental group – the preparation Betamint; animals of control group get basic diet. At the second phase, a group of healthy calves, as well as control and two experimental groups of patients were formed. The first group was treated with Intecol in combination with the probiotic Vetom. Calves of the second group – the preparation Intecoll, but in a complex with electrolyte solution Electrosol oral. The calves of the control group were treated with 4% solution of gentamicin sulfate and 30% solution of natrium thiosulfate. It was found out that during the dry period, cows were often subjected to metabolic disorders, and the causes of digestive disorders in calves were associated with a metabolic disorders in cows as a result of malnutrition. In the first stage of the research, the data obtained show the stimulatory effect of Betamint on the processes of hemopoiesis of the cows. In other side, the probable magnification increase hemoglobin in the blood of cows can be explained by the influence of the components of the preparation on the metabolism of proteins. In cows of the first experimental group, which received the mineral supplement of Sapocorm, there were no probable changes in these indices. The next stage of the work was aimed at studying the effectiveness of preparation for the treatment of sick calves. In the analysis of biochemical parameters, was obtained low effectiveness of control group treatment, which was manifested by a slight increase in the biochemical parameters of the blood of diseased calves, which indicates an ineffective action of preparation. In the treatment of calves in the first group, the general condition was normal within 4–5 days of treatment. This indicates the complex effect of compounds and other elements that are components of the preparation Intecol and probiotic Vetom, on restoring the structure and functions of digestion. An analysis of the biochemical parameters of the blood of the second group when treated with the preparation Intecol and Electrosol oral plagued the normalization of the general state occurred within 2–3 days of treatment. The revealed changes indicate the complex influence of nutrients, which are components of the preparation Electrosol oral. On the basis o","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80867417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An analysis of literary sources on issues of a property of the blood and hemostasis suggests that this problem is a significant consideration given to human medicine. There is ongoing research on the properties of blood and systems of hemostasis in female animals at the time of feeding the fetus, their impact on the indices of reproductive function and viability of the offspring remains beyond the attention of researchers. The goal of the work was to study the dynamics of haematological parameters in cows of Holstein Black-and-White breed during the dry period. The research was carried out on the cows in the dynamics of the physiological course of the dry period (from the 8th to the 9th months of calving). Hematologic studies of blood samples taken from the cows in the course of the dry period were carried out according to generally accepted methods. The results of our research indicated that deep- calving cows show a decrease in the number of red blood cells, an increase in the number of leukocytes, an increase in the oxygen capacity of the blood and an increase in the colour index. The haemoglobin content and energy potential increased, the number of lymphocytes and neutrophils with segmental nucleus increased, and the number of neutrophils with a stick-shaped nucleus decreased as well. Consequently, the characteristics features of homeostasis of deep-calving cows are: reducing the number of erythrocytes and increasing the number of leukocytes, increasing the oxygen capacity of the blood and increasing the color index, hemoglobin content and energy potential; an increasing the number of lymphocytes, segmental neutrophils, as well as reducing neutrophils with a stick-shaped nucleus. Further work will have a focus on determining the haematological parameters of high-yielding cows in the post-partum period for studying the overall resistance of the body, as well as the prediction and correction of obstetric pathology.
{"title":"Haematological indicators in high-produced cows in dynamics of dry period","authors":"L. Koreyba","doi":"10.32718/NVLVET9307","DOIUrl":"https://doi.org/10.32718/NVLVET9307","url":null,"abstract":"An analysis of literary sources on issues of a property of the blood and hemostasis suggests that this problem is a significant consideration given to human medicine. There is ongoing research on the properties of blood and systems of hemostasis in female animals at the time of feeding the fetus, their impact on the indices of reproductive function and viability of the offspring remains beyond the attention of researchers. The goal of the work was to study the dynamics of haematological parameters in cows of Holstein Black-and-White breed during the dry period. The research was carried out on the cows in the dynamics of the physiological course of the dry period (from the 8th to the 9th months of calving). Hematologic studies of blood samples taken from the cows in the course of the dry period were carried out according to generally accepted methods. The results of our research indicated that deep- calving cows show a decrease in the number of red blood cells, an increase in the number of leukocytes, an increase in the oxygen capacity of the blood and an increase in the colour index. The haemoglobin content and energy potential increased, the number of lymphocytes and neutrophils with segmental nucleus increased, and the number of neutrophils with a stick-shaped nucleus decreased as well. Consequently, the characteristics features of homeostasis of deep-calving cows are: reducing the number of erythrocytes and increasing the number of leukocytes, increasing the oxygen capacity of the blood and increasing the color index, hemoglobin content and energy potential; an increasing the number of lymphocytes, segmental neutrophils, as well as reducing neutrophils with a stick-shaped nucleus. Further work will have a focus on determining the haematological parameters of high-yielding cows in the post-partum period for studying the overall resistance of the body, as well as the prediction and correction of obstetric pathology.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"96 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75456396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There has been a massive tendency for cats to be kept as pets in Ukraine in recent years. The frequency of their diseases has also increased at the same time. Cats most often come into the homes of people from the street, from volunteers, rarely from nurseries. Due to this, Doctors often receive cats ill for microsporia, which is caused by fungi of the genus Microsporum and is one of the most common anthropozoonous diseases. The treatment process is carried out by a complex method. Analysis of the recommendations of various authors on the treatment of microsporia indicates the lack of immunostimulants in the conduct of a complex of therapeutic and preventive measures. Hematological studies were performed in order to find out the immune reactivity of the cats' organism during microsporia. The research was conducted on clinically healthy and cats ill for microspores. The obtained results of research showed that in cats with microsporia changes in morphological composition of blood were characterized by signs of anemia, leukopenia and lymphocytopenia. Changes in the structure of neutrophils were found in the type of vacuum and toxigenic grains in cats ill for microsporia. The toxic grains of neutrophils occur inside the cell as a result of physicochemical changes in the protein structure of the cytoplasm. Such cells can not provide phagocytosis of foreign agents and thus reduce the immune activity of the organism in cats ill for microsporium. A marked change in the shape of erythrocytes, which is characteristic of anemia, that is, erythrocytes-octantocytes with corneas by Jolly inclusions. Jolly's bodies are the remnants of the nucleus that have survived in erythrocytes because of the broken destruction of the normoblast nucleus. The obtained results indicate that the course and manifestation of microsporia in cats affects the immune status of the organism.
{"title":"Changes of hematological parameters of in blood in cats ill with microsporium","authors":"Y. Martyniv, Ia. V. Kisera","doi":"10.32718/NVLVET9313","DOIUrl":"https://doi.org/10.32718/NVLVET9313","url":null,"abstract":"There has been a massive tendency for cats to be kept as pets in Ukraine in recent years. The frequency of their diseases has also increased at the same time. Cats most often come into the homes of people from the street, from volunteers, rarely from nurseries. Due to this, Doctors often receive cats ill for microsporia, which is caused by fungi of the genus Microsporum and is one of the most common anthropozoonous diseases. The treatment process is carried out by a complex method. Analysis of the recommendations of various authors on the treatment of microsporia indicates the lack of immunostimulants in the conduct of a complex of therapeutic and preventive measures. Hematological studies were performed in order to find out the immune reactivity of the cats' organism during microsporia. The research was conducted on clinically healthy and cats ill for microspores. The obtained results of research showed that in cats with microsporia changes in morphological composition of blood were characterized by signs of anemia, leukopenia and lymphocytopenia. Changes in the structure of neutrophils were found in the type of vacuum and toxigenic grains in cats ill for microsporia. The toxic grains of neutrophils occur inside the cell as a result of physicochemical changes in the protein structure of the cytoplasm. Such cells can not provide phagocytosis of foreign agents and thus reduce the immune activity of the organism in cats ill for microsporium. A marked change in the shape of erythrocytes, which is characteristic of anemia, that is, erythrocytes-octantocytes with corneas by Jolly inclusions. Jolly's bodies are the remnants of the nucleus that have survived in erythrocytes because of the broken destruction of the normoblast nucleus. The obtained results indicate that the course and manifestation of microsporia in cats affects the immune status of the organism.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80882920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Skin diseases in dogs and cats occupy one of the leading places among the diseases in these species of animals. The purpose of this work was to monitor the most common contagious skin diseases in dogs and cats (otodicosis, demodicosis, dermatomycosis) during 2018 in Odessa. To solve the goal several tasks were set: to find out the species and breed structure of dogs and cats otodicosis diseases; to find out the breed structure of dogs demodicosis diseases; to find out the species and breed structure of dogs and cats dermatomycosis diseases; to determine the seasonality of otodicosis, demodicosis and dermatomycosis of dogs and cats in Odessa city during 2018. The material was provided by the data concerning sick animals registered in logs on the basis of two veterinary clinics (Gabi and Aibolit) in Odessa. These logs data were entered in the tables and analyzed statistically. For the experimental period, 134 animals were infected with contagious skin diseases, among them 51 animals were infected with otodicosis, 34 animals – with demodicosis, and 49 animals-with dermatodicosis (trichophytosis and microsporia). More cats than dogs suffered from otodicosis and demodicosis. The largest number of animals infected with otodicosis is represented by cats of Metis breed (54.2%), cats of Scottish Fold breed amounted 8.3%, cats of Persian, Maine Coon and British Shorthairs breeds – by 6.3%. Dogs of Métis breed suffered from demodicosis largely, it accounted (29.4%), dogs of Pug breed accounted 14.7%, dogs of French Bulldog breed – 11.8%. Non pedigree cats – 86.2% and non pedigree dogs – 35% suffered with dermatomycosis. Otodicosis was registered every month, except January. The increase of disease was noted in June, August, October and December. Demodicosis was registered every month, except January. The increase of disease was noted in March, September, October and December. Dermatomycosis was registered every month. The increase of disease was noted in March-April and August-December. In order to control the epizootic situation regarding contagious skin diseases of carnivorous animals in Odessa city further systematic monitoring must be carried out.
{"title":"Monitoring of contagious skin diseases of dogs and cats in Odessa","authors":"A. Iovenko, G. Koval","doi":"10.32718/NVLVET9328","DOIUrl":"https://doi.org/10.32718/NVLVET9328","url":null,"abstract":"Skin diseases in dogs and cats occupy one of the leading places among the diseases in these species of animals. The purpose of this work was to monitor the most common contagious skin diseases in dogs and cats (otodicosis, demodicosis, dermatomycosis) during 2018 in Odessa. To solve the goal several tasks were set: to find out the species and breed structure of dogs and cats otodicosis diseases; to find out the breed structure of dogs demodicosis diseases; to find out the species and breed structure of dogs and cats dermatomycosis diseases; to determine the seasonality of otodicosis, demodicosis and dermatomycosis of dogs and cats in Odessa city during 2018. The material was provided by the data concerning sick animals registered in logs on the basis of two veterinary clinics (Gabi and Aibolit) in Odessa. These logs data were entered in the tables and analyzed statistically. For the experimental period, 134 animals were infected with contagious skin diseases, among them 51 animals were infected with otodicosis, 34 animals – with demodicosis, and 49 animals-with dermatodicosis (trichophytosis and microsporia). More cats than dogs suffered from otodicosis and demodicosis. The largest number of animals infected with otodicosis is represented by cats of Metis breed (54.2%), cats of Scottish Fold breed amounted 8.3%, cats of Persian, Maine Coon and British Shorthairs breeds – by 6.3%. Dogs of Métis breed suffered from demodicosis largely, it accounted (29.4%), dogs of Pug breed accounted 14.7%, dogs of French Bulldog breed – 11.8%. Non pedigree cats – 86.2% and non pedigree dogs – 35% suffered with dermatomycosis. Otodicosis was registered every month, except January. The increase of disease was noted in June, August, October and December. Demodicosis was registered every month, except January. The increase of disease was noted in March, September, October and December. Dermatomycosis was registered every month. The increase of disease was noted in March-April and August-December. In order to control the epizootic situation regarding contagious skin diseases of carnivorous animals in Odessa city further systematic monitoring must be carried out.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82668857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The immunological aspects of lactation of cows are investigated in this work. The authors present modern scientific data on the local immune protection of the mammary gland of cows. The purpose of the research was to improve the laboratory methods of diagnosis of mastitis and to study the cytological composition of the secretion of the breast of the cows for subclinical and purulent catarrhal inflammation of the udder. The cytological differentiation of milk cells was carried out according to its own developed, tested and patented method. Our experimental data convincingly confirm that in subclinical and purulent-catarrhal mastitis in cows there are significant changes in changes in the cytological composition and the system of local immune defense of the mammary gland. An important role in inflammation of the mammary gland is played by neutrophilic granulocytes that migrate to the parenchyma at the beginning of the clinical pathology (with subclinical inflammation) and release a range of inflammatory mediators in the inflammation zone. Subclinical mastitis of cows is accompanied by a sharp increase in the total somatic cells count in the secretion of the mammary gland. This is due to the activity of neutrophilic granulocytes, monocytes of histiocytes. Corresponding changes were found in the cytological balance of immunocompetent cells of the secretion of cows at from subclinical mastitis. The most significant shift was noted in the index of migratory activity of neutrophils (P < 0.01), which is a specific “indicator” indicator of inflammatory response in the body of diseased animals. Subclinical inflammation was also manifested by a decrease in the migration activity of lymphocytes. At purulent-catarrhal mastitis of cows there is a significant increase in the number of somatic cells. Also, the migration activity of neutrophils and histiocytes in the pathologic process zone is increasing and changes in the balance of immune cells are established. Thus, our experimental data convincingly confirm that in subclinical and purulent-catarrhal mastitis in cows there are significant changes in the system of local immune defense of the mammary gland. It has an important diagnostic value and also needs immunocorrection in the treatment process.
{"title":"Changes in cellular factors of local immunity udder of cows with mastitis","authors":"M. Zhelavskyi, O. Dmytriv, V. Mizyk","doi":"10.32718/NVLVET9309","DOIUrl":"https://doi.org/10.32718/NVLVET9309","url":null,"abstract":"The immunological aspects of lactation of cows are investigated in this work. The authors present modern scientific data on the local immune protection of the mammary gland of cows. The purpose of the research was to improve the laboratory methods of diagnosis of mastitis and to study the cytological composition of the secretion of the breast of the cows for subclinical and purulent catarrhal inflammation of the udder. The cytological differentiation of milk cells was carried out according to its own developed, tested and patented method. Our experimental data convincingly confirm that in subclinical and purulent-catarrhal mastitis in cows there are significant changes in changes in the cytological composition and the system of local immune defense of the mammary gland. An important role in inflammation of the mammary gland is played by neutrophilic granulocytes that migrate to the parenchyma at the beginning of the clinical pathology (with subclinical inflammation) and release a range of inflammatory mediators in the inflammation zone. Subclinical mastitis of cows is accompanied by a sharp increase in the total somatic cells count in the secretion of the mammary gland. This is due to the activity of neutrophilic granulocytes, monocytes of histiocytes. Corresponding changes were found in the cytological balance of immunocompetent cells of the secretion of cows at from subclinical mastitis. The most significant shift was noted in the index of migratory activity of neutrophils (P < 0.01), which is a specific “indicator” indicator of inflammatory response in the body of diseased animals. Subclinical inflammation was also manifested by a decrease in the migration activity of lymphocytes. At purulent-catarrhal mastitis of cows there is a significant increase in the number of somatic cells. Also, the migration activity of neutrophils and histiocytes in the pathologic process zone is increasing and changes in the balance of immune cells are established. Thus, our experimental data convincingly confirm that in subclinical and purulent-catarrhal mastitis in cows there are significant changes in the system of local immune defense of the mammary gland. It has an important diagnostic value and also needs immunocorrection in the treatment process.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87205048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. K. Zezekalo, S. B. Pedera, T. Buslik, K. Pochernyaev
In recent times, the number of species of chlamydia associated with a number of inflammatory diseases in animals has expanded considerably. Moreover, in addition to chlamydia, attention was attracted by Сhlamydia-like organisms, which are not only dangerous for animals, but also carry a zoonotic threat. Currently, the most studied Chlamydia-like organisms are Waddlia chondrophila and Parachlamydia acanthamoebae. They are associated with diseases of the reproductive and respiratory systems in cattle and humans. Considering the danger to animals and the zoonotic threat of chlamydia-like organisms along with the absence of tools to discover them in Ukraine, the aim of our work was to develop a PCR test for the identification and species differentiation of Waddlia chondrophila. Conservative 16S rRNA genes were chosen as target genes for developing a PCR test system for identifying Waddlia chondrophila. Primers were selected specifically to be able to create multiplex combinations with the previously developed PCR test for Parachlamydia acanthamoebae. Both primers were designed with the same physical characteristics to provide simultaneous amplification under the same conditions in single or multiplex PCR. For the specificity evaluation of the primers, a panel of following DNA samples was used: Waddlia chondrophila, Parachlamydia acanthamoebae, Chlamydia avium, Chlamydia pecorum, Chlamydia abortus, Chlamydia psittaci, Chlamydia suis, Chlamydia caviae, Clavochlamydia salmonicola, Piscichlamydia salmonis. PCR product of 88 base pairs (b.p.) was formed during amplification only when the Waddlia chondrophila control DNA was present in the sample, as was expected. The small size of the PCR product theoretically allows the use of this pair of oligonucleotide primers for real-time PCR tests. After testing on clinical samples, developed PCR test system for identifying and species differentiation of Waddlia chondrophila can be used by scientists for extensive monitoring, by veterinary medicine doctors to clarify the diagnosis, and might be introduced into the practice of laboratories of veterinary and humane medicine.
{"title":"PCR-test for identification and species differentiation of Waddlia chondrophila","authors":"V. K. Zezekalo, S. B. Pedera, T. Buslik, K. Pochernyaev","doi":"10.32718/NVLVET9325","DOIUrl":"https://doi.org/10.32718/NVLVET9325","url":null,"abstract":"In recent times, the number of species of chlamydia associated with a number of inflammatory diseases in animals has expanded considerably. Moreover, in addition to chlamydia, attention was attracted by Сhlamydia-like organisms, which are not only dangerous for animals, but also carry a zoonotic threat. Currently, the most studied Chlamydia-like organisms are Waddlia chondrophila and Parachlamydia acanthamoebae. They are associated with diseases of the reproductive and respiratory systems in cattle and humans. Considering the danger to animals and the zoonotic threat of chlamydia-like organisms along with the absence of tools to discover them in Ukraine, the aim of our work was to develop a PCR test for the identification and species differentiation of Waddlia chondrophila. Conservative 16S rRNA genes were chosen as target genes for developing a PCR test system for identifying Waddlia chondrophila. Primers were selected specifically to be able to create multiplex combinations with the previously developed PCR test for Parachlamydia acanthamoebae. Both primers were designed with the same physical characteristics to provide simultaneous amplification under the same conditions in single or multiplex PCR. For the specificity evaluation of the primers, a panel of following DNA samples was used: Waddlia chondrophila, Parachlamydia acanthamoebae, Chlamydia avium, Chlamydia pecorum, Chlamydia abortus, Chlamydia psittaci, Chlamydia suis, Chlamydia caviae, Clavochlamydia salmonicola, Piscichlamydia salmonis. PCR product of 88 base pairs (b.p.) was formed during amplification only when the Waddlia chondrophila control DNA was present in the sample, as was expected. The small size of the PCR product theoretically allows the use of this pair of oligonucleotide primers for real-time PCR tests. After testing on clinical samples, developed PCR test system for identifying and species differentiation of Waddlia chondrophila can be used by scientists for extensive monitoring, by veterinary medicine doctors to clarify the diagnosis, and might be introduced into the practice of laboratories of veterinary and humane medicine.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87534751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
For the purposes of the adrenal gland’s study, 27 four months old male rabbits (Oryctolagus cuniculus) of Termond White breed were selected. Based on electrocardiography and variation pulsometry results, three types of autonomous tonus were determined in animals: sympathicotonia (ST), normotonia (NT) and parasympathicotonia (PS), which formed the basis for the division of animals into three groups. All rabbits were subjected to euthanasia and histological preparations were made from their adrenal glands. Apart from the main adrenal gland, accessory adrenal gland was detected in five purposes animals, including three ST rabbits, one NT rabbit and one PS rabbit. Also, four accessory adrenal glands were revealed in one ST rabbit simultaneously. In fact, these accessory adrenal glands became the main object of the further morphological research. The obtained historical indicators accessory adrenal glands were compared to the ones from various animal groups (CT with NT and CT with PS), as well as to the indicators of the main adrenal gland in each group. Statistical analysis of the received data was performed only in the group of ST animals. Accessory adrenal gland of ST rabbits is represented by two zones: zona glomerulosa and zona fasciculata, the area of which is 52% and 48% respectively. In PS and NT rabbits, the glands are formed by zona glomerulosa only. Investigating the accessory adrenal gland’s cellular component, it was found that NT rabbits have the largest cell area in zona glomerulosa, while PS rabbits have the smallest one. At the same time, the area of nucleus has the largest values in ST animals and the smallest ones in PS animals likewise. Сomparing zona glomerulosa and zona fasciculata in ST rabbits, it was found that cell size differs significantly, while the area of nucleus is almost identical. The nuclear-cytoplasmic ratio in zona glomerulosa cells is the largest for PS rabbits. The smallest ratio values were detected among the NT rabbits. Obviously, ST rabbits occupy an intermediate position. Unlike the main adrenal gland, the cells of accessory adrenal gland are of a smaller size and а denser location of cells in all groups of animals. The conducted studies allow concluding that the typological features of the autonomous tonus affect the morphology of accessory adrenal gland.
{"title":"Histological characteristics of accessory adrenal glands of rabbits with different types of autonomous tonus","authors":"M. Zakrevska, A. Tybinka","doi":"10.32718/NVLVET9322","DOIUrl":"https://doi.org/10.32718/NVLVET9322","url":null,"abstract":"For the purposes of the adrenal gland’s study, 27 four months old male rabbits (Oryctolagus cuniculus) of Termond White breed were selected. Based on electrocardiography and variation pulsometry results, three types of autonomous tonus were determined in animals: sympathicotonia (ST), normotonia (NT) and parasympathicotonia (PS), which formed the basis for the division of animals into three groups. All rabbits were subjected to euthanasia and histological preparations were made from their adrenal glands. Apart from the main adrenal gland, accessory adrenal gland was detected in five purposes animals, including three ST rabbits, one NT rabbit and one PS rabbit. Also, four accessory adrenal glands were revealed in one ST rabbit simultaneously. In fact, these accessory adrenal glands became the main object of the further morphological research. The obtained historical indicators accessory adrenal glands were compared to the ones from various animal groups (CT with NT and CT with PS), as well as to the indicators of the main adrenal gland in each group. Statistical analysis of the received data was performed only in the group of ST animals. Accessory adrenal gland of ST rabbits is represented by two zones: zona glomerulosa and zona fasciculata, the area of which is 52% and 48% respectively. In PS and NT rabbits, the glands are formed by zona glomerulosa only. Investigating the accessory adrenal gland’s cellular component, it was found that NT rabbits have the largest cell area in zona glomerulosa, while PS rabbits have the smallest one. At the same time, the area of nucleus has the largest values in ST animals and the smallest ones in PS animals likewise. Сomparing zona glomerulosa and zona fasciculata in ST rabbits, it was found that cell size differs significantly, while the area of nucleus is almost identical. The nuclear-cytoplasmic ratio in zona glomerulosa cells is the largest for PS rabbits. The smallest ratio values were detected among the NT rabbits. Obviously, ST rabbits occupy an intermediate position. Unlike the main adrenal gland, the cells of accessory adrenal gland are of a smaller size and а denser location of cells in all groups of animals. The conducted studies allow concluding that the typological features of the autonomous tonus affect the morphology of accessory adrenal gland.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76800034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Immune system of reproductive organs of animals is represented by a number of cellular and humoral protection factors. Recently, the attention of scientists was attracted by the role of immunocompetent cells, which integrally provide immune homeostasis at all stages of the reproductive function. The purpose of the work was to study the cellular factors of immune defense of mucous membranes of genital organs of dogs and cats. The cytological composition and determination of immunological parameters of vaginal microscope slide were determined by patented methods. Local immunity was determined by the parameters of extracellular protection mechanisms, which consisted in the study of the Oxygen-dependent potential of neutrophil granulocytes (NBT-test) and their ability to form extracellular traps (NETs). Our studies have found that in different stages of the sexual cycle in beach and cats, along with cytological changes in epithelial cells, changes occur in phagocytic protection. In particular, in the post-infusion period there was an increase in the number of intermediate and basal epithelial cells on the surface of which were adhered microorganisms. Along with epithelial cells, activated phagocytes were grouped. Neutrophils showed cytochemical reactivity in the NBT-test, and also formed NETs. At the same time, activated phagocytes absorbed microorganisms, and some epithelial cells formed specific cytoplasmic inclusions, which obviously also has an important role in the antimicrobial immunity of the mucosa. Some neutrophilic granulocytes were in a state of apoptosis. During the sexual cycle in the organism of dogs and cats there are morphology and functional changes in the genital organs, which was accompanied by a change in the cytological picture of vaginal microscope slides. It was established that the antimicrobial protection system plays an important role in the neutrophilic granulocytes, the functional activity of which also has a change. The main mechanisms of extracellular antimicrobial protection of microphages are realized with the involvement of Oxygen species in the excretion system of active forms and the formation of neutrophilic extracellular traps. Epithelial cells are also involved in the formation of immune homeostasis of genital mucous membranes.
{"title":"Study of innate factors in the local immune defense of the genital organs of dogs and cats","authors":"M. Zhelavskyi","doi":"10.32718/NVLVET9317","DOIUrl":"https://doi.org/10.32718/NVLVET9317","url":null,"abstract":"Immune system of reproductive organs of animals is represented by a number of cellular and humoral protection factors. Recently, the attention of scientists was attracted by the role of immunocompetent cells, which integrally provide immune homeostasis at all stages of the reproductive function. The purpose of the work was to study the cellular factors of immune defense of mucous membranes of genital organs of dogs and cats. The cytological composition and determination of immunological parameters of vaginal microscope slide were determined by patented methods. Local immunity was determined by the parameters of extracellular protection mechanisms, which consisted in the study of the Oxygen-dependent potential of neutrophil granulocytes (NBT-test) and their ability to form extracellular traps (NETs). Our studies have found that in different stages of the sexual cycle in beach and cats, along with cytological changes in epithelial cells, changes occur in phagocytic protection. In particular, in the post-infusion period there was an increase in the number of intermediate and basal epithelial cells on the surface of which were adhered microorganisms. Along with epithelial cells, activated phagocytes were grouped. Neutrophils showed cytochemical reactivity in the NBT-test, and also formed NETs. At the same time, activated phagocytes absorbed microorganisms, and some epithelial cells formed specific cytoplasmic inclusions, which obviously also has an important role in the antimicrobial immunity of the mucosa. Some neutrophilic granulocytes were in a state of apoptosis. During the sexual cycle in the organism of dogs and cats there are morphology and functional changes in the genital organs, which was accompanied by a change in the cytological picture of vaginal microscope slides. It was established that the antimicrobial protection system plays an important role in the neutrophilic granulocytes, the functional activity of which also has a change. The main mechanisms of extracellular antimicrobial protection of microphages are realized with the involvement of Oxygen species in the excretion system of active forms and the formation of neutrophilic extracellular traps. Epithelial cells are also involved in the formation of immune homeostasis of genital mucous membranes.","PeriodicalId":21677,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnology","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87034328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：