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How early can nonpregnant ewes be detected based on the blood flow of the corpus luteum? 根据黄体的血流量,可以在多早检测到未孕母羊?
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.smallrumres.2024.107211
Leandro Becalete Rizzoni , Miller Pereira Palhão , João Henrique Moreira Viana , Vinícius Oliveira Souza , José da Pascoa Nascimento Neto , Jairo Pereira Neves

The study aimed to characterize morpho-functional changes in corpus luteum (CL) in pregnant and non-pregnant sheep, propose diagnostic criteria for non-pregnancy, and assess diagnostic accuracy around the expected luteolysis period. Crossbred ewes (n = 34) underwent estrous synchronization, divided into G1 (unmated, n = 20) and G2 (mated, n = 14). The day of ovulation was considered as day 0 (D0). Luteal dynamic was monitored from D0 to D17, with blood samples collected every 72 h for progesterone (P4). Pregnancy was definitively diagnosed at day 30. Analyses included CL area (CLA), CL blood flow (CLBF), CLBF:CLA ratio, and serum P4. Statistical models considered group, day, and interactions. Accuracy for early pregnancy diagnosis (days 12–17) was assessed. CLBF:CLA ratio (14.3 ± 8.1 vs. 20.3 ± 10.7; 10.2 ± 10.4 vs. 17.8 ± 9.4; 1.3 ± 4.1 vs. 16.0 ± 8.2%), CLBF (0.41 ± 0.3 vs. 0.49 ± 0.3; 0.26 ± 0.3 vs. 0.45 ± 0.3; 0.06 ± 0.2 vs. 0.34 ± 0.2 cm2), and CLA (2.5 ± 1.1 vs. 2.3 ± 0.9; 1.9 ± 1.1 vs. 2.3 ± 1.1; 0.9 ± 1.2 vs. 2.0 ± 1.2 cm2) differed (P < 0.05) between pregnant and nonpregnant ewes at days 13, 14, and 16 after ovulation, respectively. The early diagnosis of nonpregnancy achieved accuracy values > 90% from day 15 on for CLBF and CLBF:CLA ratio, but not for CLA. At this day, CLBF and CLBF:CLA ratio were circa 50-fold greater in pregnant than in nonpregnant ewes (p < 0.0001). No ewe classified as in the 1st or 2nd quartiles for CL endpoints or P4 concentration was confirmed as pregnant at day 30. In summary, the diagnosis of nonpregnancy based on the objective evaluation of CLBF could be performed in sheep as early as at day 15 after ovulation.

该研究旨在描述妊娠羊和非妊娠羊黄体(CL)的形态功能变化,提出非妊娠的诊断标准,并评估预期黄体溶解期前后的诊断准确性。杂交母羊(34 只)进行了发情同步化,分为 G1(未交配,20 只)和 G2(已交配,14 只)。排卵日被视为第 0 天(D0)。从第 0 天到第 17 天监测黄体动态,每 72 小时采集血液样本检测孕酮(P4)。第 30 天确诊妊娠。分析包括CL面积(CLA)、CL血流(CLBF)、CLBF:CLA比率和血清P4。统计模型考虑了组别、天数和交互作用。评估了早孕诊断(第 12-17 天)的准确性。CLBF:CLA 比值(14.3 ± 8.1 vs. 20.3 ± 10.7;10.2 ± 10.4 vs. 17.8 ± 9.4;1.3 ± 4.1 vs. 16.0 ± 8.2%)、CLBF(0.41 ± 0.3 vs. 0.49 ± 0.3;0.26 ± 0.3 vs. 0.45 ± 0.3;0.06 ± 0.2 vs. 0.34 ± 0.2 cm2)和 CLA(2.5 ± 1.1 vs. 2.3 ± 0.9; 1.9 ± 1.1 vs. 2.3 ± 1.1; 0.9 ± 1.2 vs. 2.0 ± 1.2 cm2)的差异(P < 0.05)。从排卵后第 15 天开始,未孕早期诊断对 CLBF 和 CLBF:CLA 比值的准确率达到 90%,但对 CLA 的准确率不高。在这一天,怀孕母羊的 CLBF 和 CLBF:CLA 比率比未怀孕母羊高出约 50 倍(p < 0.0001)。在第 30 天时,没有一头 CL 终点或 P4 浓度处于第 1 或第 2 四分位数的母羊被确认为怀孕。总之,基于CLBF的客观评估,最早可在排卵后第15天对绵羊进行未孕诊断。
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引用次数: 0
Corrigendum to “Postprocedural clinical and laboratory evaluation of sheep submitted to different intrabdominal pressures during laparoscopy” [SSRN Electron. J. 228 (2023) 107092] 更正:"对腹腔镜手术中承受不同腹压的绵羊进行手术后临床和实验室评估" [SSRN Electron. J. 228 (2023) 107092]
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.smallrumres.2024.107212
L. V. de Gouvêa, T. R. S. Leite, Paulo R L do Nascimento, André Luis de Souza Teixeira, Paulo S. C. da Silva, José Aurelino Damasceno Ferreira Filho, Maria Fernanda Oliveira Ferreira Nunes, Juliana Jeunon Senna, Raphael Delecrodi Leonardo Pereira, Michel Abdalla Helayel, N. X. de Alencar, Camila Marinelli Martins, P. L. Silva, Andreza Amaral da Silva, D. Lessa
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引用次数: 0
Population genomic and haplotype block structure in Attappady Black and Malabari goats of South India 南印度 Attappady 黑山羊和马拉巴里山羊的种群基因组和单倍型区块结构
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.smallrumres.2024.107225
Marykutty Thomas, Radhika Gopalakrishnan, Thirupathy Venkattachalapathy, Aravindakshan Thazhathuveetil
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引用次数: 0
Autosomal and sex-linked genetic parameters for body weight gain and its relationship with efficiency-related traits in sheep 绵羊增重的常染色体和性连锁遗传参数及其与效率相关性状的关系
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.smallrumres.2024.107228
F. Ghafouri-Kesbi, Moradpasha Eskandarinasab
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引用次数: 0
Diplotype-Based Assessment of GNRHR Gene Polymorphisms and Their Role in Determining Litter Size at Birth in the Malabari Goat Population of South India 基于 Diplotype 的南印度马拉巴里山羊群体 GNRHR 基因多态性评估及其在决定产仔数方面的作用
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-01 DOI: 10.1016/j.smallrumres.2024.107229
S.K. Saranya, Marykutty Thomas, T. Aravindakshan, R. T. Venkatachalapathy, Jinty Sukumaran, Raji Kanakkaparambil
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引用次数: 0
A protocol to assess the welfare of dairy ewes: From science to the field 奶牛母羊福利评估协议:从科学到实践
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-01-29 DOI: 10.1016/j.smallrumres.2024.107209
Pierre-Alexandre Jolly , Elise Rouyer , Laetitia Bru , Lorie Marin , Elise Meillac , Gabrielle Foubert , Mathilde Roman , Andrew Arthur Ponter , Olivier Patout , Alline de Paula Reis

There is growing societal demand for improved animal welfare. The stakeholders in the industry are also increasingly interested in information and expertise in this area. There is a scientific consensus that an objective assessment should be the foundation for discussions and actions in favour of better animal welfare. However, only a few protocols exist and most of them have been developed for meat-producing sheep and/or are more adapted for an extensive farming context and/or do not include all the five freedoms of animal welfare. Therefore, references are still scarce especially in the field of dairy ewe welfare. This makes the transition from science to practice difficult. The present study was carried out bearing two objectives in mind: (1) to develop a feasible protocol (EBBEL – Evaluation du Bien-être des Brebis en Elevage Laitier / Assessment of sheep welfare in dairy farms) to assess the welfare of dairy ewes and (2) to implement the protocol on a large scale (n = 81 dairy ewe farms under semi-intensive system) in the Roquefort region of France. The animals and the farms were examined in terms of satisfaction of 26 welfare indicators. The results were very informative about the points of excellence but also about those that need to be improved. The database that we developed can serve the scientific community when comparing different production contexts. Beyond the technical results, stakeholder participation in the design process was clearly positive for acceptance of the protocol in the field.

社会对改善动物福利的需求日益增长。行业内的利益相关者也越来越关注这方面的信息和专业知识。科学界已达成共识,即客观评估应作为讨论和行动的基础,以改善动物福利。然而,目前仅有少数几个协议,其中大部分是针对肉羊制定的,并且/或者更适合于大规模养殖,并且/或者并不包括动物福利的所有五项自由。因此,特别是在奶牛母羊福利领域,参考资料仍然很少。这使得从科学到实践的过渡变得困难。本研究有两个目标:(1) 制定一个可行的方案(EBBEL - Evaluation du Bien-être des Brebis en Elevage Laitier / 奶牛场绵羊福利评估)来评估奶牛场母羊的福利;(2) 在法国罗克福地区大规模实施该方案(n = 81 个半集约化系统下的奶牛场母羊)。根据 26 项福利指标的满意度对动物和牧场进行了考察。调查结果表明,我们的牧场既有优秀之处,也有需要改进之处。我们开发的数据库可为科学界比较不同的生产环境提供服务。除技术成果外,利益相关者参与设计过程显然对该方案在实地的接受度具有积极意义。
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引用次数: 0
Effect of vitamin D on oxidative stress indices and ram semen parameters 维生素 D 对氧化应激指数和公羊精液参数的影响
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-01-28 DOI: 10.1016/j.smallrumres.2024.107207
Y. Teymoori , M. Mohri , P. Mirshokraei

High vitamin D3 receptor and vitamin D3 metabolizing enzyme expression during spermatogenesis reflects the importance of vitamin D in fertility. This project aimed to study the effects of vitamin D3 supplementation on semen parameters and the oxidative system of rams. Twenty sexually active, mature rams were randomly selected. Rams were evenly (based on their age and live weight) categorized into four groups: control group (CG), low-dose vitamin D3 treatment group (LDG), high-dose vitamin D3 treatment group (HDG), and normal group (NG). The CG, LDG, and HDG were kept in a dark place for three months, and the vitamin D3 supplement was removed from the diet to cause vitamin D3 deficiency. The NG was placed in free sunlight, and a vitamin D3 supplement was added to their diet; thus, the vitamin D3 serum levels were normal in this group. After vitamin D3 deficiency (< 75 nmol/L vitamin D in the serum), rams received 2000IU/kg vitamin D3 in the LDG. Rams in the HDG received 20000IU/kg of vitamin D3. The rams in the CG group did not receive vitamin D3 supplementation. Sperm collection was performed three times in the morning for three consecutive months. The results showed a relationship between vitamin D3 serum level, semen parameters, and oxidative stress indices. After the treatment, vitamin D3 serum levels in LDG (128.4 ± 17.3 nmol/L) and HDG (119.3 ± 10.6 nmol/L) were statistically (P < 0.05) different from CG (42.9 ± 3.6 nmol/L). Treatment with both doses of vitamin D3 significantly improved sperm parameters (P < 0.05). Sperm viability (95 ± 6% in LDG, 95 ± 4% in HDG, and 61 ± 1.1% in CG), sperm PMI (85 ± 5% in LDG and 84 ± 5% in HDG, whereas 51 ± 8% in CG), sperm morphology (9 ± 3% in LDG and 10 ± 2% in HDG in contrast with 28 ± 6% in CG), and DNA fragmentation index (10 ± 3% in LDG, 11 ± 1% in HDG, and 31 ± 7% in CG) were statistically different (P < 0.05). Treatment with both doses of vitamin D3 substantially (P < 0.05) declined malondialdehyde concentration (1.2 ± 0.4 µmol/L in LDG and 1 ± 0.3 µmol/L in HDG in contrast with 9.7 ± 0.9 µmol/L in CG) and total oxidant status level (9.9 ± 3.8 µmol H2O2 equiv/L in LDG and 10 ± 3.6 µmol H2O2 equiv/L in HDG whereas 77 ± 10.6 µmol H2O2 equiv/L in CG). However, total antioxidant capacity concentration (4.17 ± 0.61 µmol fe2+/L in LDG and 4.86 ± 0.62 µmol fe2+/L in HDG while 1.00.29 µmol fe2+/L in CG) and total thiol concentration (231.9 ± 23.8 µmol/mg protein in LDG and 250.6 ± 25.9 µmol/mg protein in HDG in contrast with 31.5 ± 11.2 µmol/mg protein in CG) statistically (P < 0.05) increase in LDG and HDG. In conclusion, vitamin D3 supplementation improved semen quality in rams by its

精子发生过程中维生素D3受体和维生素D3代谢酶的高表达反映了维生素D在生育中的重要性。本项目旨在研究补充维生素 D3 对公羊精液参数和氧化系统的影响。随机挑选了 20 只性欲旺盛的成熟公羊。根据年龄和活重将公羊平均分为四组:对照组(CG)、低剂量维生素 D3 治疗组(LDG)、高剂量维生素 D3 治疗组(HDG)和正常组(NG)。将 CG、LDG 和 HDG 放在黑暗处三个月,并从饮食中去除维生素 D3 补充剂,以造成维生素 D3 缺乏。将 NG 放在自由阳光下,并在其饮食中添加维生素 D3 补充剂;因此,该组的维生素 D3 血清水平正常。维生素 D3 缺乏后(血清中维生素 D 含量为 75 nmol/L),低密度组的公羊每公斤摄入 2000IU 的维生素 D3。HDG 组的公羊每公斤摄入 20000IU 的维生素 D3。CG组的公羊不补充维生素D3。连续三个月在早晨进行三次精子采集。结果显示,维生素 D3 血清水平、精液参数和氧化应激指数之间存在关系。治疗后,LDG(128.4 ± 17.3 nmol/L)和 HDG(119.3 ± 10.6 nmol/L)的维生素 D3 血清水平与 CG(42.9 ± 3.6 nmol/L)相比有统计学差异(P < 0.05)。两种剂量的维生素 D3 都能显著改善精子参数(P < 0.05)。精子活力(LDG 为 95 ± 6%,HDG 为 95 ± 4%,CG 为 61 ± 1.1%)、精子 PMI(LDG 为 85 ± 5%,HDG 为 84 ± 5%,CG 为 51 ± 8%)、精子形态(LDG 为 9 ± 3%,HDG 为 10 ± 2%,CG 为 28 ± 6%)和 DNA 碎片指数(LDG 为 10 ± 3%,HDG 为 11 ± 1%,CG 为 31 ± 7%)均有统计学差异(P < 0.05)。两种剂量的维生素 D3 都能显著降低丙二醛浓度(LDG 为 1.2 ± 0.4 µmol/L,HDG 为 1 ± 0.3 µmol/L,而 CG 为 9.7 ± 0.9 µmol/L)和总氧化状态水平(LDG 为 9.9 ± 3.8 µmol H2O2 当量/L,HDG 为 10 ± 3.6 µmol H2O2 当量/L,而 CG 为 77 ± 10.6 µmol H2O2 当量/L)(P < 0.05)。然而,总抗氧化能力浓度(LDG 为 4.17 ± 0.61 µmol fe2+/L,HDG 为 4.86 ± 0.62 µmol fe2+/L,CG 为 1.02±0.29 µmol fe2+/L)和总硫醇浓度(231.9±23.8µmol/mg蛋白,HDG为250.6±25.9µmol/mg蛋白,而CG为31.5±11.2µmol/mg蛋白)在LDG和HDG中均有统计学意义(P <0.05)的增加。总之,补充维生素 D3 可通过维生素 D3 受体作用和减少氧化应激改善公羊的精液质量。
{"title":"Effect of vitamin D on oxidative stress indices and ram semen parameters","authors":"Y. Teymoori ,&nbsp;M. Mohri ,&nbsp;P. Mirshokraei","doi":"10.1016/j.smallrumres.2024.107207","DOIUrl":"10.1016/j.smallrumres.2024.107207","url":null,"abstract":"<div><p>High vitamin D<sub>3</sub> receptor and vitamin D<sub>3</sub> metabolizing enzyme expression during spermatogenesis reflects the importance of vitamin D in fertility. This project aimed to study the effects of vitamin D3 supplementation on semen parameters and the oxidative system of rams. Twenty sexually active, mature rams were randomly selected. Rams were evenly (based on their age and live weight) categorized into four groups: control group (CG), low-dose vitamin D<sub>3</sub> treatment group (LDG), high-dose vitamin D<sub>3</sub> treatment group (HDG), and normal group (NG). The CG, LDG, and HDG were kept in a dark place for three months, and the vitamin D<sub>3</sub> supplement was removed from the diet to cause vitamin D<sub>3</sub> deficiency. The NG was placed in free sunlight, and a vitamin D<sub>3</sub> supplement was added to their diet; thus, the vitamin D<sub>3</sub> serum levels were normal in this group. After vitamin D<sub>3</sub> deficiency (&lt; 75 nmol/L vitamin D in the serum), rams received 2000IU/kg vitamin D<sub>3</sub> in the LDG. Rams in the HDG received 20000IU/kg of vitamin D<sub>3</sub>. The rams in the CG group did not receive vitamin D<sub>3</sub> supplementation. Sperm collection was performed three times in the morning for three consecutive months. The results showed a relationship between vitamin D<sub>3</sub> serum level, semen parameters, and oxidative stress indices. After the treatment, vitamin D<sub>3</sub> serum levels in LDG (128.4 ± 17.3 nmol/L) and HDG (119.3 ± 10.6 nmol/L) were statistically (P &lt; 0.05) different from CG (42.9 ± 3.6 nmol/L). Treatment with both doses of vitamin D<sub>3</sub> significantly improved sperm parameters (P &lt; 0.05). Sperm viability (95 ± 6% in LDG, 95 ± 4% in HDG, and 61 ± 1.1% in CG), sperm PMI (85 ± 5% in LDG and 84 ± 5% in HDG, whereas 51 ± 8% in CG), sperm morphology (9 ± 3% in LDG and 10 ± 2% in HDG in contrast with 28 ± 6% in CG), and DNA fragmentation index (10 ± 3% in LDG, 11 ± 1% in HDG, and 31 ± 7% in CG) were statistically different (P &lt; 0.05). Treatment with both doses of vitamin D<sub>3</sub> substantially (P &lt; 0.05) declined malondialdehyde concentration (1.2 ± 0.4 µmol/L in LDG and 1 ± 0.3 µmol/L in HDG in contrast with 9.7 ± 0.9 µmol/L in CG) and total oxidant status level (9.9 ± 3.8 µmol H<sub>2</sub>O<sub>2</sub> equiv/L in LDG and 10 ± 3.6 µmol H<sub>2</sub>O<sub>2</sub> equiv/L in HDG whereas 77 ± 10.6 µmol H<sub>2</sub>O<sub>2</sub> equiv/L in CG). However, total antioxidant capacity concentration (4.17 ± 0.61 µmol fe<sup>2+</sup>/L in LDG and 4.86 ± 0.62 µmol fe<sup>2+</sup>/L in HDG while 1.0<sup>2±</sup>0.29 µmol fe<sup>2+</sup>/L in CG) and total thiol concentration (231.9 ± 23.8 µmol/mg protein in LDG and 250.6 ± 25.9 µmol/mg protein in HDG in contrast with 31.5 ± 11.2 µmol/mg protein in CG) statistically (P &lt; 0.05) increase in LDG and HDG. In conclusion, vitamin D<sub>3</sub> supplementation improved semen quality in rams by its","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139632087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Survival analysis of genetic and non-genetic factors influencing lamb survival of different sheep breeds 影响不同绵羊品种羔羊存活率的遗传和非遗传因素的生存分析
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-01-24 DOI: 10.1016/j.smallrumres.2024.107206
Shanbel Besufkad , Aschalew Abebe , Tesfaye Getachew , Shenkute Goshme , Asfaw Bisrat , Ayele Abebe , Tesfaye Zewdie , Leulseged Alemayehu , Ashenafi Kebede , Solomon Gizaw

Data records of 3800 Dorper, Dorper × Menz and Menz lambs born from 2011 to 2023 were used to determine non-genetic factors and to estimate variance components of lamb survival at Debre Birhan Agricultural Research Center, Ethiopia. The traits investigated were lamb survival from birth to 90, 180, 365 and 90 to 180 days of age. The data was subjected to analysis using the Weibull proportional hazard model. The mortality rates of lambs at different age intervals (90, 180, 365, and 90 to 180 days) were determined for three breeds: Dorper, Dorper × Menz, and Menz. The respective mortality rates for each breed were as follows: 23.96%, 36.85%, 48.72%, and 15.47% for Dorper; 23.64%, 37.33%, 56.32%, and 17.00% for Dorper × Menz; and 12.98%, 23.44%, 39.31%, and 11.44% for Menz respectively. Breed of lamb, year of birth and lamb weight at birth significantly (P < 0.05) affected risk of lamb mortality during all the periods studied. On the other hand, except from 90 to 180 days of age, lamb inbreeding coefficient, birth type, parity of dam and season of birth significantly (P < 0.05) affected lamb risk of death at all studied periods. However, sex of lamb had a significant (P < 0.05) effect during birth to yearling and weaning to six months of age. Dorper and Dorper × Menz lambs had higher (P < 0.05) risk of death compared with the local breed (Menz lamb). The likelihood of mortality significantly decreased as the weight of the lamb at birth increased, transitioning from very low values (less than 2 kg) to higher values (4.00 kg and above). Lambs with a higher inbreeding coefficient (above 6.25%) exhibited the highest risk of death (2.13) compared to lambs with a zero inbreeding coefficient. Lambs born from younger (nulliparous) and older dams (5 parity and above) faced a higher risk of death. The heritability estimates for lamb survival during the birth to yearling age period ranged from 0.004 for Menz to 0.25 for Dorper, indicating variability in the genetic influence on this trait between the two breeds. While direct selection for lamb survival may be challenging due to low heritability specifically for Menz sheep, implementing measures such as improved lamb care, accounting for seasonal variations, and indirect selection based on birth weight can lead to notable improvements in lamb survival rates within the research center. The significant and alarming lamb mortality rates observed in the research center necessitate the immediate implementation of measures and strategies.

埃塞俄比亚 Debre Birhan 农业研究中心利用 2011 年至 2023 年间出生的 3800 只 Dorper、Dorper × Menz 和 Menz 羔羊的数据记录来确定非遗传因子并估计羔羊存活率的方差成分。调查的性状为羔羊从出生到 90、180、365 和 90 至 180 日龄的存活率。数据采用 Weibull 比例危险模型进行分析。测定了三个品种不同日龄间隔(90 天、180 天、365 天和 90 天至 180 天)的羔羊死亡率:Dorper、Dorper × Menz 和 Menz。各品种的死亡率分别为Dorper的死亡率分别为23.96%、36.85%、48.72%和15.47%;Dorper × Menz的死亡率分别为23.64%、37.33%、56.32%和17.00%;Menz的死亡率分别为12.98%、23.44%、39.31%和11.44%。在所有研究期间,羔羊品种、出生年份和出生时羔羊体重对羔羊死亡风险有显著影响(P < 0.05)。另一方面,除 90 至 180 日龄外,羔羊近交系数、出生类型、母羊奇数和出生季节对所有研究期间的羔羊死亡风险均有显著影响(P < 0.05)。然而,羔羊性别对出生至一岁龄和断奶至 6 个月龄期间的影响明显(P < 0.05)。与本地品种(Menz羔羊)相比,Dorper和Dorper × Menz羔羊的死亡风险更高(P < 0.05)。随着羔羊出生时体重的增加,死亡的可能性明显降低,从极低值(低于 2 千克)过渡到较高值(4.00 千克及以上)。与近交系数为零的羔羊相比,近交系数较高(高于 6.25%)的羔羊死亡风险最高(2.13)。较年轻(无产仔)和较年长(5 胎及以上)的母羊所产羔羊的死亡风险较高。从出生到一岁期间羔羊存活率的遗传率估计值从 Menz 的 0.004 到 Dorper 的 0.25 不等,表明这两个品种对这一性状的遗传影响存在差异。由于孟氏绵羊的遗传率较低,直接选择羔羊存活率可能具有挑战性,但采取改善羔羊护理、考虑季节性变化以及基于出生体重的间接选择等措施,可以显著提高研究中心的羔羊存活率。研究中心观察到的羔羊死亡率非常高,令人担忧,因此有必要立即采取措施和策略。
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引用次数: 0
The effects of age of castration on carcass characteristics, meat quality and fatty acid profile of finishing South African Mutton Merino lambs 阉割年龄对南非美利奴羊育成羔羊胴体特征、肉质和脂肪酸组成的影响
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-01-19 DOI: 10.1016/j.smallrumres.2024.107205
Ndyebo Skele , Willem Cornelius Augustyn , Hester Adri O’Neill , Arnold Hugo , Ockert Bernard Einkamerer

This study compared the effect of either keeping South African Mutton Merino (SAMM) ram lambs intact, or castrated with age applicable methods on their carcass characteristics, meat quality and fatty acid composition of subcutaneous fat (SCF) and intramuscular fat (IMF). Ten lambs per treatment were castrated early at one week of age (EAR) with an elastrator band (4.4 kg ± 0.78 kg: mean ± SD), another 10 lambs at eight weeks of age (late castration - LAT) with a burdizzo (23.6 kg ± 2.26 kg: mean ± SD), whereas another ten lambs were left intact (CON). The two castration methods used were based on cost, ethical prescriptions and to mimic commercial operations. The lambs were housed individually in 30 pens (1.404 m2) for the duration of the study. Animals were housed on an elevated slatted wooden floor in the same metabolic building with an extractor fan to promote ventilation. At the start of the feeding trial, the age of the lambs was on average two and a half months with a live weight of 24.3 kg ± 4.10 kg (mean ± SD). The lambs were fed a standard commercial finishing diet for 67 days and slaughtered when they reached approximately 44.2 kg ± 6.35 kg (mean ± SD) live weight. After slaughter, carcass characteristics (cold carcass) and meat quality were evaluated. The left 3-rib cut was dissected (9th through 11th rib) and the muscle percentage for CON lambs was higher compared to both EAR and LAT lambs. Therefore, CON lambs produced leaner carcasses compared to EAR and LAT lambs. Castration status had no effect on meat Warner-Bratzler shear force (WBSF), consumer sensory analysis nor colour. For proximate composition, CON lambs had higher moisture compared to LAT lambs while LAT had higher dry matter compared to CON lambs. Both, EAR and LAT lamb’s meat had higher organic matter compared to CON lamb’s meat, but these differences were negligible. For IMF in the longissimus thoracic et lumborum muscle, CON lambs’ meat had higher total PUFA, α-linolenic acid, total PUFA n-6, linoleic acid, PUFA:SFA ratio and PUFA:MUFA ratio than meat from both EAR and LAT lambs. Eicosapentaenoic acid (EPA) and total PUFA n-3 were both higher for meat from CON lambs than LAT lamb’s meat, while palmitic acid and conjugated linoleic acid (CLA) were higher for EAR lamb’s meat than CON lamb’s meat and EAR lambs than LAT lamb’s meat, respectively. For SCF, CON lambs had higher heptadecenoic acid and linolelaidic acid than LAT lambs. The calculated atherogenicity index (AI) of SCF was higher for LAT lambs compared to CON lambs. Limited effects of castration age on the possible health benefits of SAMM lamb meat were proven in the current study. It is however suggested that for a higher production response and carcass muscle content, to either keep SAMM rams intact or at least postpone castration for as long as possible.

本研究比较了保持南非美利奴羊肉(SAMM)公羔完整或采用适用年龄的阉割方法对其胴体特征、肉质以及皮下脂肪(SCF)和肌内脂肪(IMF)的脂肪酸组成的影响。每次处理有 10 只羔羊在一周龄时使用阉割带进行早期阉割(EAR)(4.4 千克 ± 0.78 千克:平均值 ± SD),另有 10 只羔羊在八周龄时使用阉割带进行晚期阉割(LAT)(23.6 千克 ± 2.26 千克:平均值 ± SD),而另外 10 只羔羊则保持完好无损(CON)。采用这两种阉割方法是基于成本、道德规定和模仿商业操作的考虑。在研究期间,羔羊被单独饲养在 30 个栏(1.404 平方米)中。动物被饲养在同一栋新陈代谢大楼的高架板条木地板上,并配有抽风机以促进通风。饲喂试验开始时,羔羊的平均年龄为两个半月,活重为 24.3 kg ± 4.10 kg(平均值 ± SD)。羔羊在标准商业育成饲料中饲喂 67 天,活重达到约 44.2 千克 ± 6.35 千克(平均值 ± 标准差)时屠宰。屠宰后,对胴体特征(冷胴体)和肉质进行了评估。剖开左侧 3 肋(第 9 至 11 肋),与 EAR 和 LAT 羔羊相比,CON 羔羊的肌肉百分比更高。因此,与 EAR 和 LAT 羔羊相比,CON 羔羊的胴体更瘦。阉割状态对肉的华氏剪切力(WBSF)、消费者感官分析和颜色没有影响。就近似成分而言,CON羔羊的水分高于LAT羔羊,而LAT羔羊的干物质高于CON羔羊。与CON羔羊肉相比,EAR和LAT羔羊肉的有机物含量更高,但这些差异可以忽略不计。在胸长肌和腰长肌的IMF中,CON羔羊肉的总PUFA、α-亚麻酸、总PUFA n-6、亚油酸、PUFA:SFA比率和PUFA:MUFA比率均高于EAR和LAT羔羊肉。CON羔羊肉的二十碳五烯酸(EPA)和PUFA n-3总量均高于LAT羔羊肉,而EAR羔羊肉的棕榈酸和共轭亚油酸(CLA)分别高于CON羔羊肉和EAR羔羊肉。在SCF方面,CON羔羊的十七碳烯酸和亚油酸高于LAT羔羊。经计算,LAT羔羊的SCF致动脉粥样硬化指数(AI)高于CON羔羊。目前的研究证明,阉割年龄对 SAMM 羊肉可能带来的健康益处影响有限。不过,为了获得更高的生产响应和胴体肌肉含量,建议保持 SAMM 公羊完整,或至少尽可能推迟阉割时间。
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引用次数: 0
Study of the relationship between the presence of milky cysts, udder imbalances, udder morphological traits and somatic cell counts 乳状囊肿的存在、乳房失衡、乳房形态特征和体细胞数之间关系的研究
IF 1.8 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-01-15 DOI: 10.1016/j.smallrumres.2024.107203
Renée de Cremoux , Maxime Legris , Virginie Clément , Apolline Bailly-Salins , Marine Minier

In France, among the goat breeders on genetic improvement programs, udder scores are recorded annually on primiparous does. New phenotypes include imbalances of the udder (166,929 records since 2016) and milky cysts (100,079 records since 2018). The objectives of this study were to (1) describe the frequency and intensity of these new phenotypes, (2) evaluate their associations with udder conformation traits, (3) determine their impact on milk somatic cell counts (SCC) and dairy production performance. Udder imbalances were defined using a 4-level grid according to the height difference between udder halves. Milky cysts were described as either isolated or numerous. The data were compared with information on mammary conformation (9 traits, each being evaluated on a scale of 1 to 9 with the exception of the length of the teat), SCC (geometric mean calculated over 250 days of lactation) and milk production performance obtained over a reference period of 250 days. In 90.8% (of the cases, udder imbalances were absent or very small, with the most pronounced imbalances (≥10 cm difference between glands) accounting present in only 1.1% of goats. The greatest deficiencies were associated with low udders and poorly attached udders (fore and rear attachments). SCC increased with the degree of imbalance, from 589,000 to 1349,000 cel/ml between no or slight imbalance and an imbalance of ≥10 cm or more. Milky cysts were recorded in 1.9% of the scored udders. Udders with cysts were characterized by poor fore attachments. Likely of traumatic origin, milky cysts were nevertheless associated with increased SCC: 646,000 vs. 912,000 cells/ml for no cysts and numerous cysts, respectively. The hypothesis of a deterioration in milking conditions for the most severe cases remains to be assessed. Finally, impacts on milk production or protein and fat contents reached - 14.1%, - 9.7% and −12.9% respectively for unbalanced udders compared to balanced ones. They were not noticeable for the presence of cysts.

在法国,山羊育种者在遗传改良计划中,每年都会记录初产母羊的乳房评分。新的表型包括乳房不平衡(自 2016 年以来有 166929 条记录)和乳汁囊肿(自 2018 年以来有 10079 条记录)。本研究的目的是:(1)描述这些新表型的频率和强度;(2)评估它们与乳房构象性状的关联;(3)确定它们对牛奶体细胞数(SCC)和奶牛生产性能的影响。根据乳房两半之间的高度差,使用 4 级网格来定义乳房不平衡。乳腺囊肿分为孤立囊肿和大量囊肿。将这些数据与乳房构型(9 个性状,每个性状的评估等级为 1 到 9,乳头长度除外)、SCC(250 天泌乳期计算的几何平均数)和 250 天参考期的产奶性能等信息进行比较。90.8%的山羊没有乳房失衡或失衡很小,只有1.1%的山羊存在最明显的失衡(腺体间相差≥10厘米)。最大的不足与乳房过低和乳房附着不良(前后附着)有关。SCC随失衡程度的增加而增加,在无失衡或轻微失衡与失衡≥10厘米或以上之间,SCC从589,000 cel/ml增加到1349,000 cel/ml。在 1.9% 的评分乳房中记录到乳汁囊肿。有囊肿的乳房前部附着力差。乳汁囊肿可能是外伤造成的,但与 SCC 的增加有关:无囊肿和多囊肿的 SCC 分别为 646,000 cells/ml 和 912,000 cells/ml。最严重病例的挤奶条件恶化这一假设仍有待评估。最后,与平衡乳房相比,不平衡乳房对牛奶产量或蛋白质和脂肪含量的影响分别为-14.1%、-9.7%和-12.9%。对囊肿的影响并不明显。
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Small Ruminant Research
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