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Fluorescent ZnO Quantum Dot Probe to Study Glucose–Glucose Oxidase Interaction via Fluorescence Resonance Energy Transfer 荧光氧化锌量子点探针通过荧光共振能量转移研究葡萄糖-葡萄糖氧化酶相互作用
Pub Date : 2020-05-01 DOI: 10.1166/sl.2020.4232
A. K. Verma, Z. Ansari
In this work, emphasis is to utilize FRET method to analyze GOx and glucose interaction using fluorescent ZnO QDs as the probes. Fluorescent ZnO and ZnOext QDs were synthesized using sol– gel technique and green synthesis method as donor and acceptor nanoprobes. Structural, optical and morphological characterization of QDS were carried out using UV-visible absorption, fluorescence, FTIR, XRD and field emission scanning electron microscopy. Band gap estimated from Tauc plot is 3.49 and 3.35 eV for ZnO and ZnOext QDs and XRD reveal Wurtzite structure of grown crystals. Systematic absorption study for ZnO–GOx and ZnOext–GOx reveal association constant of –8.3361 M–1 and –2.57646 M –1 for ZnO and ZnOext using Benesi-Hildebrand plot. The binding constant obtained from Stern-Volmer equation is 1.0466 μM –1 and 1.97 μM –1 for ZnO/ZnOext–GOx conjugate and suggest static quenching in the system. CD spectroscopy reveal native state of protein in conjugate sytem. Constant Förster radius (Ro) in ZnOext–GOx system suggest the average 〈κ2〉 as 2/3 and is independent of donor–acceptor distance as normally assumed in FRET system varies from ∼4.5% in ZnO–GOx and ∼5% in ZnOext–GOx system. It indicate better energy transferrin earlier system than formal. The physiological range of glucose from 60 mg–440 mg/dl using this technique in which ZnOext–GOx as donor probe exhibit better response towards glucose than ZnO–GOx system and correlated to the presence of phyotochemicals on the QD surface.
在这项工作中,重点是利用荧光氧化锌量子点作为探针,利用FRET方法分析GOx和葡萄糖的相互作用。采用溶胶-凝胶技术和绿色合成法分别制备了ZnO和ZnOext荧光量子点作为供体和受体纳米探针。利用紫外可见吸收、荧光、红外光谱、x射线衍射和场发射扫描电镜对QDS进行了结构、光学和形态表征。通过tac图估计ZnO和ZnOext量子点的带隙分别为3.49和3.35 eV, XRD显示生长晶体的纤锌矿结构。利用Benesi-Hildebrand图对ZnO - gox和ZnOext - gox进行系统吸收研究,发现ZnO和ZnOext的结合常数分别为-8.3361 M -1和-2.57646 M -1。由Stern-Volmer方程得到ZnO/ ZnOext-GOx共轭物的结合常数分别为1.0466 μM -1和1.97 μM -1,表明ZnO/ ZnOext-GOx在体系中处于静态猝灭状态。CD光谱揭示了共轭体系中蛋白质的天然状态。在ZnOext-GOx系统中,恒定Förster半径(Ro)表明平均< κ2 >为2/3,与FRET系统中通常假设的供体-受体距离无关,在ZnOext-GOx系统中为~ 4.5%,在ZnOext-GOx系统中为~ 5%。表明早期系统的能量传递优于正式系统。以ZnOext-GOx作为供体探针,该技术对葡萄糖的生理反应范围为60 mg - 440 mg/dl,比ZnO-GOx系统对葡萄糖的反应更好,并与量子点表面存在的植物化学物质相关。
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引用次数: 5
Development of a New Nontoxic Thiazole Derivative Dye for Cell Labeling by Using Nanoparticles 新型无毒噻唑衍生物纳米细胞标记染料的研制
Pub Date : 2020-04-01 DOI: 10.1166/sl.2020.4215
Havva Nur Gurbuz, P. U. Arpaci, A. B. Sariguney, Erdal Kocabaş, A. Coşkun, E. Cagil
Fluorescent labeling of L-929 fibroblast cells was studied by using newly synthesized ligand which was named as 2- N -methylamino-5-(pyrene-1-yl)-thiazole (ABS 40). The maximum fluorescence intensity of ABS 40 was detected at an excitation and emission wavelengths of 350 nm and 400 nm. Fluorescence intensity of the ligand was changed by the addition of DNA from calf thymus (CT-DNA) and human serum albumin (HSA). Both biomolecules and ABS 40 formed complexes that changed the fluorescence intensity of the ligand at the given wavelengths. Cytotoxicity tests were done with XTT analysis. L-929 fibroblast cells were cultured in the presence of ABS 40 to show cell labeling. Results showed that ABS 40 at 318 μM was labeled the cells at an excitation wavelength of 403.7 nm by using confocal microscopy. in vivo cell labeling was also confirmed by using Ct-DNA immobilized iron nanoparticles.
采用新合成的2- N -甲氨基-5-(芘-1-基)-噻唑(ABS 40)配体对L-929成纤维细胞进行荧光标记研究。ABS 40在激发和发射波长分别为350 nm和400 nm时检测到最大荧光强度。通过加入小牛胸腺DNA (CT-DNA)和人血清白蛋白(HSA)改变配体的荧光强度。生物分子和ABS 40形成的复合物改变了配体在给定波长下的荧光强度。细胞毒性试验采用XTT分析。L-929成纤维细胞在ABS - 40的作用下培养,显示细胞标记。结果表明,在共聚焦显微镜下,318 μM的ABS 40在403.7 nm的激发波长下被标记。用Ct-DNA固定铁纳米颗粒也证实了体内细胞标记。
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引用次数: 0
Improved Sensitivity of Dielectric Modulated Junctionless Transistor for Nanoscale Biosensor Design 纳米尺度生物传感器设计中介电调制无结晶体管灵敏度的提高
Pub Date : 2020-04-01 DOI: 10.1166/sl.2020.4224
Shradhya Singh, S. Bala, B. Raj, B. Raj
This work has proposed a device i.e., Dielectric Modulated (DM) Junctionless Transistor which is utilizes as Label-Free (LF) electrical characteristic detection of bio-molecules. The electrical characteristics used for the detection of biomolecules are electric field, surface potential, drain current and threshold voltage (Vth). Due to immobilization of biomolecules in the cavity region, the threshold voltage change in comparison to the absence of biomolecule, which is utilizes as the sensitivity metric. The sensitivity of biomolecule detection can be enhanced by using asymmetric gate operation of the device. In asymmetric mode the degree of sensitivity is almost five times higher than that of the symmetric mode of operation.
本工作提出了一种用于生物分子无标签(Label-Free, LF)电特性检测的器件,即介电调制(DM)无结晶体管。用于检测生物分子的电特性是电场、表面电位、漏极电流和阈值电压(Vth)。由于生物分子固定在腔区,阈值电压相对于没有生物分子时发生变化,并以此作为灵敏度指标。采用非对称栅操作可以提高生物分子检测的灵敏度。在非对称模式下,灵敏度几乎是对称模式下的5倍。
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引用次数: 9
A Rotation-Speed Measurement System Based on Magneto-Inductive Effect 基于磁感应效应的转速测量系统
Pub Date : 2020-04-01 DOI: 10.1166/sl.2020.4219
Tao Wang, Bicong Wang, Yuyi Chen, Y. Luo
There are few reports on high rotational speed measurement of brushless direct current (DC) motors based on giant magneto-inductive effect. In this study, a rotational speed measurement system based on giant magneto-inductive effect is established for measuring the rotational speeds of DC motors. Successive sawtooth waves are found when rotating shaft is close to the GMI sensor. Through using the magneto-inductive sensing system, different high rotational speeds (1500∼24000 r/min) of the motor are accurately measured. Therefore, this giant magneto-inductive sensor may be used for high rotational speed measurement of motors.
基于巨磁感应效应的无刷直流电动机高转速测量目前报道较少。本文建立了一种基于巨磁感应效应的直流电机转速测量系统。当转轴靠近GMI传感器时,发现连续的锯齿波。通过使用磁感应传感系统,精确测量电机的不同高转速(1500 ~ 24000 r/min)。因此,该传感器可用于电机的高转速测量。
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引用次数: 0
Porcelain Insulator Inspection Using Infrared Thermography 用红外热成像技术检测瓷绝缘体
Pub Date : 2020-04-01 DOI: 10.1166/sl.2020.4229
P. I. Lerantzis, P. Tsarabaris, N. Theodorou
Inspection with thermography is a non-invasive, non-destructive process used in Power Systems facilities. Most temperature signatures that are not in the visible light range can be measured using thermal cameras, thus enabling the search of thermal behavior of porcelain insulators. Overheating can help to detect problems, concerning the proper operation of insulators. The precision of temperature measurement using an infrared camera is influenced by the value of emissivity parameter that is set on camera. In the present study the influence of distance and angle between the camera and the insulator on emissivity parameter value selection in camera is examined experimentally.
热成像检查是一种非侵入性、非破坏性的过程,用于电力系统设施。大多数不在可见光范围内的温度特征可以用热成像仪测量,从而可以搜索瓷绝缘体的热行为。过热可以帮助发现问题,涉及绝缘体的正确操作。红外热像仪测温的精度受热像仪上设置的发射率参数的影响。实验研究了相机与绝缘体之间的距离和角度对相机发射率参数选择的影响。
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引用次数: 0
A Comprehensive Analysis of Various Structural Parameters of the Untreated Calcium Phosphate from Two Moroccan Mining Zones 摩洛哥两矿区原生磷酸钙各结构参数综合分析
Pub Date : 2020-04-01 DOI: 10.1166/sl.2020.4216
Ikram Labtaini, K. El-hami
X-ray diffraction (XRD) and Raman spectroscopy analyses were performed on untreated calcium phosphate samples of the two mining zones of Morocco: Benguerir and Khouribga. XRD has been examined for the determination of the structure parameters and their textures. However, a comparative study was conducted between the two types of phosphate to implement the observed structural differences. XRD Results showed that the comparison between the two samples reveals significant differences at the level of the refinement parameters, crystallite size and microstrain but no changes in the crystal system. Raman analysis assignments are given for powdered samples of the organically important compound, calcium phosphate, in the 0–3600 cm–1 range. The wavenumber positions of Raman bands were different and changed between the two different samples. The results may be of significance in understanding the relationship between XRD analysis and the parameters of material structure.
对摩洛哥Benguerir和Khouribga两个矿区未经处理的磷酸钙样品进行了x射线衍射(XRD)和拉曼光谱分析。用XRD测定了材料的结构参数和织构。然而,我们对两种类型的磷酸盐进行了比较研究,以实现所观察到的结构差异。XRD结果表明,两种样品在细化参数、晶粒尺寸和微应变水平上存在显著差异,但晶体体系没有变化。拉曼分析作业给出了粉末样品的有机重要化合物,磷酸钙,在0-3600厘米- 1范围内。两种不同样品的拉曼带波数位置不同且有所变化。研究结果对理解XRD分析与材料结构参数之间的关系具有重要意义。
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引用次数: 0
Power Management in a Hybrid Grid Involving Photo Voltaic and Proton Exchange Membrane Fuel Cell 光伏与质子交换膜燃料电池混合电网的电源管理
Pub Date : 2020-04-01 DOI: 10.1166/sl.2020.4220
N. Shetty, R. L. Chakrasali
The ever growing energy demand explored the generation of power by distributed resources. The distributed resources like photovoltaic, wind, fuel cell etc. are available in nature at free of cost. Photovoltaic cell is used to produce electrical energy by tracking energy from solar irradiation. Perturbation and Observing method is one of the option to track the concentrated power from solar irradiation. It is a clean source, but intermittent in nature. This is overcome by integrating a fuel cell (FC), the new energy source. Proton Exchanging Membrane (PEM) fuel cell is integrated with PV, and this hybrid DC source is integrated to micro grid using power electronic interfacing devices. The design of proper controller operation makes it to work in grid connected mode. The simulation is performed using Matlab-Simulink environment and results validates the performance of hybrid system.
不断增长的能源需求探索了分布式资源发电。光伏、风能、燃料电池等分布式资源在自然界中是免费的。光伏电池是通过跟踪太阳辐照的能量来产生电能的。摄动观测法是跟踪太阳辐照聚光的一种方法。它是一种清洁能源,但本质上是断断续续的。这是通过集成新能源燃料电池(FC)来克服的。质子交换膜(PEM)燃料电池与光伏集成,混合直流电源通过电力电子接口装置与微电网集成。合理的控制器操作设计使其能够在并网模式下工作。在Matlab-Simulink环境下进行了仿真,结果验证了混合系统的性能。
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引用次数: 1
Quality Monitoring of Saccharum officinarum (Sugarcane) Using Image Analysis 利用图像分析技术监测甘蔗糖精的质量
Pub Date : 2020-04-01 DOI: 10.1166/sl.2020.4221
Jayavanta Shakthi Poorna Sekar, R. Jayaparvathy
The yield of the sugarcane depends on the quality of the seed used for plantation. The traditional methods of cultivation of sugarcane by cutting random sections of a stalk called sets and sowing them for propagation purposes result in quality degradation. When a defective sugarcane part is used for propagation purposes poor quality sugarcanes are obtained. This results in wastage of a large number of resources used for cultivation purposes. In the paper, we propose an efficient method using image processing for determining the nodes of the sugarcane crop that are found suitable for the propagation of good quality sugarcane. We present the design of a hardware prototype that automatically cuts and gives the cultivator a suitable node that has to be sowed for fresh cultivation. We validate the proposed method by determining the quality of fifty samples of sugarcane stalks and observe that our method works with 93.62 percent efficiency.
甘蔗的产量取决于用于种植的种子的质量。传统的甘蔗种植方法是随意剪下一段甘蔗茎,然后播种以繁殖,结果导致质量下降。当有缺陷的甘蔗部分用于繁殖时,得到的是质量差的甘蔗。这就造成了用于种植的大量资源的浪费。在本文中,我们提出了一种有效的方法,利用图像处理来确定甘蔗作物的节点,这些节点被发现适合优质甘蔗的繁殖。我们提出了一个硬件原型的设计,它可以自动切割并给耕种者一个合适的节点,该节点必须播种以进行新鲜栽培。通过对50个甘蔗秸秆样品的质量测定,验证了该方法的有效性,效率为93.62%。
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引用次数: 0
Novel Mechatronic Sensors for Irregular Movements of Autonomous Land Vehicles 用于自主陆地车辆不规则运动的新型机电传感器
Pub Date : 2020-04-01 DOI: 10.1166/sl.2020.4218
R. Boucetta, Radwen Bahri, Saloua Bel Hadj Ali
In this paper, novel sensors using physical phenomena deduced from ultrasound waves are proposed to overcome problems of cost, availability and adaptability in the measure of position, velocity and tilt of Autonomous Land Vehicles. The true challenge for the Autonomous Land Vehicles concerns essentially the accurate perception of hazardous environments to receive faithful information about reality. Current sensors used nowadays are viewed very expensive, complicated and unable to give accurate measures without time delay. For that, our contribution is located in this context. At first, fundamental principle of ultrasonic waves and their specific physical properties are described to introduce their use in the proposed sensors. Mechatronic concepts of sensors are then detailed with the corresponding mathematical models. Indeed, online implementation and real time experiences are conducted to verify the effectiveness of measurements.
针对自动驾驶车辆位置、速度和倾斜度测量中存在的成本、可用性和适应性等问题,提出了一种基于超声波物理现象的新型传感器。自动驾驶汽车面临的真正挑战主要是对危险环境的准确感知,从而获得真实的信息。目前使用的电流传感器被认为非常昂贵,复杂且无法在没有时间延迟的情况下给出准确的测量。为此,我们的贡献就在这方面。首先,介绍了超声波的基本原理及其具体的物理性质,介绍了超声波在传感器中的应用。然后用相应的数学模型详细介绍了传感器的机电一体化概念。事实上,在线实施和实时体验被用来验证测量的有效性。
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引用次数: 0
Electrochemical Behavior and Voltammetric Determination of Losartan Using Carbon Ceramic Electrode Modified with Room Temperature Ionic Liquid 用室温离子液体修饰碳陶瓷电极测定氯沙坦的电化学行为和伏安法
Pub Date : 2020-04-01 DOI: 10.1166/sl.2020.4223
Mahzad Firouzi, M. Giahi, M. Najafi, S. Homami, Seyed Husain Hashemi Mousavi
Herein, an electrochemical sensor has been proposed for the determination of losartan (LOS) in pharmaceutical formulations. A room temperature ionic liquid, 1-butyl-3-methylimidazolium hexafluorophosphate (BMIM.PF6) was applied to fabricate a modified carbon ceramic electrode (IL-CCE). The electrochemical studies were performed by the cyclic and linear sweep voltammetry (CV and LSV), chronoamperometry and electrochemical impedance spectroscopy (EIS) techniques. The anodic peak currents were increased with the LOS concentration and indicated a linear dynamic range from 20 to 200 μM and a detection limit of 11.7 μM (S/N = 3) under the optimized conditions. Satisfactory results were obtained by analysis of LOS in pharmaceutical tablets.
本文提出了一种电化学传感器,用于测定药物制剂中的氯沙坦(LOS)。采用室温离子液体1-丁基-3-甲基咪唑六氟磷酸(bmm . pf6)制备了改性碳陶瓷电极(IL-CCE)。采用循环和线性扫描伏安法(CV和LSV)、计时电流法和电化学阻抗谱(EIS)技术进行了电化学研究。在优化条件下,阳极峰值电流随LOS浓度的增加而增大,线性动态范围为20 ~ 200 μM,检出限为11.7 μM (S/N = 3)。对片剂中的LOS进行了分析,得到了满意的结果。
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引用次数: 0
期刊
Sensor Letters
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